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The Peptide Synthesis Core Facility is a dedicated peptide synthesis facility for Simpson Querrey Institute members, NU researchers, and other local academic and commercial labs. Our facility provides customers with high quality peptides synthesized at 0.05-‐2 mmol scales and purified by reverse-‐phase HPLC. We can also carry out side-‐chain or terminal modifications such as the addition of fluorescent or biotin labels according to the customers’ research applications. In addition, we utilize the leading-‐edge Agilent 6520 Q-‐TOF LCMS system to develop and deliver a wide range of mass spectrometry services. The Peptide Synthesis Core is located on the 11th floor of the Robert H. Lurie Medical Research Center on Northwestern University’s Chicago Campus.
Simpson Querrey Institute Northwestern University Robert H. Lurie Medical Research Center 303 E. Superior Street, 11th Floor Chicago, Illinois 60611 www.sqi.northwestern.edu
about us
contact us For more information about the Peptide Synthesis Core Facility
Contact: Mark Karver, PhD Director, Peptide Synthesis Core Facility [email protected] (312) 503 6738 Christopher Tarczynski Research Technologist c-‐[email protected] (312) 503 6725 Emily Testa Research Technologist emily-‐[email protected] (312) 503 6725 www.sqi.northwestern.edu/peptidecore.html
PEPTIDE SYNTHESIS
Core
Facility
our services CUSTOM PEPTIDE SYNTHESIS Using microwave technology for enhanced yield and faster synthesis
branched peptides
cyclic peptides
long peptides
hydrophobic peptides
glycopeptides
phosphopeptides
FRET peptides
dye-‐labeled peptides
drug-‐labeled peptides
biotin-‐labeled peptides
peptides with terminal modification
MASS SPECTROMETRY Using Agilent 6520 Quadrupole Time-of-Flight (Q-TOF) LCMS
molecular weight determination of small molecules and biopolymers
peptide sequence identification and verification
characterization of synthesis products and complex mixtures
Northwestern University rates
Peptide Synthesis Automated Peptide Synthesis Setup Fee1,2 $250 per synthesis
Peptide Synthesis Multi-Peptide $295 per setup Amino Acids 0.05 mmol $5 per amino acid
0.1 mmol $8 per amino acid 0.25 mmol $11 per amino acid 0.5 mmol $13 per amino acid
1.0 mmol $16 per amino acid 2.0 mmol $20 per amino acid
Special Reagents (non-standard residues, dyes, resins) call for price Manual Peptide Synthesis Full-Service call for price Self-Service Setup fee1 $150 per synthesis
HPLC Preparatory Full3/Self-Service $85/$55 per injection
Large Scale Preparatory Full Service $215 per injection Analytical Full/Self-Service $40/$30 per injection Lyophilization (if required) $31 per port
Mass Spectrometry (MS) Molecular Weight Determination (MWD) <5 samples $12 per sample
MWD ≥ 5 samples $10 per sample MWD with Sample Preparation $22 per sample Tandem MS (MS/MS) $75 per sample LCMS <5 samples $45 per sample LCMS ≥ 5 samples $25 per sample Proteomics Study $150 per hour
Technical Assistance Consultation, training, data analysis, lyophilizer setup $100 per hour 1 Additional consumables charges will apply depending on the number of amino acids required in the process. 2 Includes synthesis setup, peptide cleavage, and MS analysis of crude peptide. 3 With each injection 40-200 mg of crude product is purified, depending on the solubility of each peptide. Recovery is usually between 5-50 mg and is often >90% pure by reverse-phase HPLC.
The Peptide Synthesis Core Facility welcomes all researchers at Northwestern University whose work involves custom peptide synthesis. For project quotes or more information, contact Mark Karver at [email protected] or 312-503-6738. September, 1, 2016