abstract book - cipharma.ufop.br · 13- débora faria silva ... 23- kelly christyne miranda pereira...
TRANSCRIPT
II INTERNACIONAL SYMPOSIUM
OF PHARMACEUTICAL SCIENCE
Pharmacy School -UFOP
ABSTRACT BOOK
October 28 and 29, 2015. Ouro Preto-MG, Brazil
http://www.cipharma.ufop.br/
2
PRESENTATION
Dear Participants,
It is an honor to invite all to the II Symposium to be held by Pharmaceutical
Science Program of Pharmacy Scholl. Taking advantage of the Symposium
success in 2013 and the importance of this action continuity, which strengthens
the program, through the integration with graduate students, with the extension
programs and amplifying the internationalization, this second edition is intended
to enlarge the audience. Graduate students linked to research and extension
projects are also called to participate and share their experiences with
CiPharma master and PhD students. The event is the result of professors’ work
in order to improve the program actions and to improve integration, inclusion
and diffusion of new knowledge and technologies, as well as to contribute to the
excellence of masters and PhD in Pharmaceutical Science. The event is
supported by a project CAPES/COFECUB, coordinated by Prof. Vanessa Carla
F. Mosqueira, by CiPharma and Pharmacy School - UFOP. So, we would like to
welcome the participants who will contribute to the achievement and success of
this event.
Thank You.
4
Symposium Organization Committee
Neila Márcia Silva Barcellos, PhD - DEFAR/UFOP
Andrea Grabe Guimarães, PhD - DEFAR/UFOP
Vanessa Carla Furtado Mosqueira, PhD - DEFAR/UFOP
Mrs. Mirela Pena Fagundes - CiPharma Secretary
Scientific Committee
Carlos Henryque Souza e Silva – Post doc CiPharma
Elton Luiz Silva – Post doc CiPharma
Giane Martins Garcia – Post doc CiPharma
Gisele Vieira Rodovalho – Post doc CiPharma
Gwenaelle Elza Nathalie Pound-Lana – Post doc CiPharma
Hygor Mezadri - CiPharma Pharmacist
Nádia das Dores Moreira – Post doc CiPharma
Renata Tupinambá Branquinho - CiPharma Pharmacist
Sidney de Almeida Ferreira - Post doc NUPEB
5
REGISTERED PARTICIPANTS
1- Adriana Cotta Cardoso Reis
2- Alessandra Hermógenes Gomes Tobias
3- Amanda Maria Silva Gonçalves
4- Ana Barbosa Zanoti
5- Ana Carolina da Silva Santos
6- Ana Carolina Moreira Souza
7- Ana Paula Amariz Silveira
8- Bruno de Rezende Vieira
9- Catarina Stivali Teixeira
10- Daiane Teixeira de Oliveira
11- Danielle Cristiane Corrêa de Paula
12- Danielle Murta Silva
13- Débora Faria Silva
14- Débora Priscila de Campos
15- Douglas Gualberto Sales Pereir
16- Fernanda Barçante Perasoli
17- Fernanda Cristina Ferrari
18- Isabela Matoso Lopes
19- Izabel Cristina Trindade
20- Jéssica Campolina de Souza
21- Josemar da Conceição Mendes
22- Juliane Morais da Fonseca
6
23- Kelly Christyne Miranda Pereira de Castro
24- Laryssa Freitas Guimarães
25- Leidiane Vieira Simões
26- Letícia Paiva de Matos
27- Lidiane Rodrigues Antunes
28- Lorena Barbosa Arrunátegui
29- Lorena de Souza Rosa
30- Lorena Ulhôa Araújo
31- Luan Silvestro Bianchini Silva
32- Luana Beatriz Araújo Vaz
33- Lucas Felipe Fernandes Bittencourt
34- Luciene Gonçalves da Paixão
35- Ludmila Correia Barbosa
36- Mariana Mafia Penido Ferreira Ribeiro
37- Mariana Reis Di Mambro
38- Mariana Trevisan Rezende
39- Michelle Karoline Conceição Miranda
40- Nayara Nascimento Toledo Silva
41- Quênia Janaína Tomaz de Castro
42- Regislainy Gomes da Silva
43- Rodrigo Chaves
44- Samara Stéfani de Castro Silva
45- Samira Fagundes de Andrade
7
46- Tamara Cristina Moreira Lopes
47- Tamires Guedes Caldeira
48- Tatiane Martins Barros
49- Tatiane Roquete Amparo
50- Thayná Vichiato Silva
51- Thaís dos Santos Paulino Soares
52- Thaís Faco Maganhoto
53- Thaís Santos Rodrigues
54- Thales de Andrade Martins
55- Thiago Magalhães Gouvea
56- Vanessa Santos
57- Vittorio Amadeus Kasten Avery
58- Zilma Schimith Ferraz Filha
8
Program
Wednesday, 28 october
Time Event
8:30 am Registration, Welcome
9 am Opening Section:
Neila Márcia Silva Barcellos – CiPharma Coordinator
Celia Maria Nunes – UFOP Vice-President
Fábio Faversani – UFOP research Head
Vanessa Carla F. Mosqueira - CAPES/COFECUB Project Coordinator
Carlos Magno de Souza Paiva – CAINT - UFOP
10:30 am Coffee break
10:45 am I Roundtable: New Knowledge to the future of cardiovascular therapeutics –
Andrea Grabe Guimaraes, PhD (DEFAR/UFOP)
1. Evaluation of cardiac function: from in vivo to cells and molecules.
Applications to cardiac diseases and drugs cardiotoxicity - Sylvain Richard,
PhD (INSERM 1046, UM1, França).
2. Heart as a pharmacological target for essential oils - Jader dos Santos
Cruz, PhD (ICB/UFMG).
3. - Study of Ang(1-7) and its analogues actions as future pharmacological
strategies for treatment of cardiovascular diseases - Andréia Carvalho
Alzamora, PhD (DECBI/UFOP).
13 pm Lunch
14 pm II Roundtable: Parasitic diseases chemotherapy - Vanessa Carla Furtado
Mosqueira, PhD (DEFAR/UFOP)
1. Andréa Teixeira de Carvalho (Rene Rachou)
2. Maria Terezinha Bahia (DECBI/UFOP)
3. Marta de Lana (DEACL/UFOP)
16 to 18 pm Painel Section I
Thursday, 28 october
Time Event
9 am Students Oral Presentation
10:30 am Coffee break
10:30 am to
12:30 pm
Painel Section II
12:30 pm Lunch
14:30 pm III Roundtable : Research on women's health
1. Vanja Maria Veloso, PhD (DEFAR/UFOP)
2. Antônio Carlos Pinto Guimarães, PhD (UFSJ)
3. Romulo Leite, PhD (DEFAR/UFOP)
16:30 pm Finish section
ABSTRACTS
1
QUALITY CONTROL IN CERVICAL
CYTOPATHOLOGY: PERFORMANCE OF RAPID
PRESCREENING, 100% RAPID REVIEW AND THE
REVIEW BASED ON CLINICAL RISK CRITERIA
Alessandra Hermógenes Gomes Tobias1,2
, Aline Costa
Vitalino2, Renata Rocha Rezende e Oliveira
2, Mariana
Trevisan Rezende1,2
, Jennefer Aparecida do
Nascimento Gonçalves2, Cláudia Martins Carneiro
1,2
1CIPHARMA/Pharmacy School/Federal University of
Ouro Preto/Ouro Preto/MG 2Cytology Sector/Pilot Laboratory of Clinical
Analysis/Pharmacy School/Federal University of Ouro
Preto/Ouro Preto/MG
Background: The Pap smear or cervical cytology
exam is a screening strategy widely used in the
prevention of cervical cancer. This examination consists
of several steps, and conducting internal quality
monitoring methods (MIQ) is indispensable to confer
quality to the final result, mainly because these
methods help to reduce false-negative. Objective: To
evaluate the performance of Rapid Prescreening, 100%
Rapid Review and the Review based on Clinical Risk
Criteria as MIQ methods. Methodology: This study
was conducted in Cytology Sector of LAPAC, between
October 2012 and October 2014. It was first performed
the Rapid Prescreening and noted in a spreadsheet if
the exam was suspicious, negative or unsatisfactory.
Subsequently was realized the routine screening of the
exam. Then all exams considered negative by the
routine screening were reviewed by the 100% Rapid
Review and the result was classified as suspicious,
negative or unsatisfactory. Exams classified as
negative by the routine screening that showed clinical
risk criteria were reviewed and classified as positive or
not after detailed review and final diagnosis. All smears
considered positive and unsatisfactory for routine
screening or suspects and unsatisfactory by MIQ
methods were referred for detailed review to confirm or
not the presence of positivity for then determinate the
final diagnosis, which was the gold standard for
calculate the MIQ method performance. It was done a
database with the results of the methods of MIQ and
final diagnosis and finally we calculated the sensitivity
and specificity for each one of the methods using the
OpenEpi program, version 3. Results: During the
period established for this work the Cytology Sector of
LAPAC received a total of 9.751 cervical cytology
exams from all Basic Health Units in the city of Ouro
Preto, Minas Gerais. Of these tests, 357 were rejected
and returned to Basic Health Units, getting a total of
9.394 to be analyzed. The Rapid Prescreening, the
100% Rapid Review and the Review based on Clinical
Risk Criteria was conducted in a total of 9.394, 8.244
and 1.381 exams, respectively. The sensitivity of the
Rapid Prescreening, the 100% Rapid Review and the
Review based on Clinical Risk Criteria was 48.2%,
42.6% and 22.6% and the specificity was 90%, 95%
and 84% respectively. Conclusion: The MIQ method
which demonstrated better performance in laboratory
routine was 100% Rapid Review.
Acknowledgment: FAPEMIG, CNPQ, CAPES E
UFOP.
2
FACTORS ASSOCIATED WITH QUALITY OF LIFE
AND HEALTH IN WOMEN IN CLIMACTERIC.
ZANOTI AB*1,MIRANDA MKC
1, VELOSO VM
1,SOUSA
JC1,RIBEIRO MMPF
1, FERREIRA MCBC
1, CARRILLO
MRG1, LIMA AA SERPA MA
1,SEBASTIAO ECO
1,
CARRILLO MRG1, LIMA AA
1 , NUNES DDC
1,COURA-
VITAR W1.
1Escola de Farmácia/UFOP, Ouro Preto – MG
*e-mail: [email protected]
With increasing of life expectancy of the population,
especially women, it is important to exchange
knowledge about the climacteric. The objective was to
disseminate knowledge about the risk factors
associated with perimenopause and menopause that
affect quality of life. This project was undertaken in the
city of Ouro Preto, in partnership with SUS, which has
as priority actions to disease prevention and health
promotion. The beneficiaries were women aged 45-60
years in different stages of menopause, which were
invited to attend lectures given by students and project
contributors. The themes covered in the lectures were
related to health-disease process, focusing on risk
factors, which occur more often during this period of
life. Among the topics covered, hypertension, diabetes,
dyslipidemia and osteoporosis were those that had
more prominence, since they arouse greater interest
from the beneficiaries. Added to this idea, other
activities have been offered to contribute to the
empowerment of the women, such as cosmetics and
10
food workshops in order to promote the redefinition of
this phase of life, awakening autonomy and interlace
the links between population, SUS and university. It is
believed that the actions taken have contributed to
improving the quality of life of these women and a more
critical and human formation of the students involved.
Thanks: The basic health Units - UBS in the city of
Ouro Preto Ouro, which has always collaborated with
our project assisting in contact with women benefited.
Financial support: FAPEMIG e Pró-reitoria de
extensão- PROEX.
3
ANTIMALARIAL EFFICACY OF ARTEMETHER
ADMINISTERED ORALLY IN NANOCAPSULES
SOUZA ACM1,VIDAL-DINIZ AT
1, ANDRADE RP
1,
SILVEIRA APA1, CASTRO Q.J.T
1, GUIMARÃES H.N
2,
RICHARD S3, MOSQUEIRA V.C.F
1, GRABE-
GUIMARÃES A1
1CiPharma, Escola de Farmácia, UFOP, Ouro Preto,
MG;
2DEE, Escola de Engenharia, UFMG, Belo Horizonte,
MG, Brazil;
3Universitè de Montpellier 1, INSERM 1046;
Introduction: Malaria is a public health disease caused
by Plasmodium sp and it is an important cause of
illness and death in children and adults in 97 malaria
endemic countries (WORLD MALARIA REPORT, 2014;
WHO, 2015). The artemisinin derivatives, as
Artemether (ATM), are an alternative to malaria
Plasmodium falciparum treatment (WHO, 2015). The
nanosystems development offers the necessary tools to
deliver active molecules to specific sites. New
nanostructures can protect the molecule from
degradation by the oral route, modify the biodistribution
and pharmacokinetic profile and bioavailability.
Objective: Evaluate the antimalarial efficacy of ATM in
nanocapsules (NC) administered orally. Methods: the
production of ATM NC was previously described
(VIDAL-DINIZ, 2014). The animals were infected with
Plasmodium berguei and three days after, treatments
were initiated. ATM formulations were administered
during 4 days, every 12 hours. The animals groups
treatment were: a) ATM NC (40, 80 and 120 mg/kg); b)
free ATM (40, 80 and 120 mg/kg); c) empty NC; d)
vehicle. The animals survival index was evaluated and
also the percentage of parasitemia after different
treatments. The animals were evaluated over 30 days.
Results: The animals treated with ATM NC showed a
significant reduction of parasitemia compared to groups
treated with the same dose of free ATM. The animals
treated with ATM NC were able to reduce the
parasitemia by 75%; 67% and 64% at 40, 80 and 120
mg/kg, respectively, compared to animals treated with
free ATM. Moreover the survival index of animals
treated with ATM NC was significantly higher than
animals treated with either control as with ATM free.
The animals treated with vehicle and empty NC
survived until the 9th and 19th day, respectively, after
the infection. The higher dose administered, 120 mg/kg,
showed 100% survival for animals treated with ATM
NC, whereas for animals treated with free ATM it was
only 67% until the 30th day. Conclusion: The ATM NC
administered orally has greater effectiveness in
reducing the parasitemia, since it was observed the
increase in animal survival index.
Funding agencies: FAPEMIG (Rede Nanobiomg-
CDS_PPM0481-13) and UFOP
Acknowledgments: CNPq; UFOP; CAPES and Project
APQ 02346-11 (FAPEMIG)
4
ASSESSMENT OF KNOWLEDGE AND PERCEPTION
OF ADOLESCENTS AND MOTHERS ABOUT HPV
AND CERVICAL CANCER IN OURO PRETO: A
PILOT STUDY
Ana Carolina da Silva Santos1, Camila de Paula Dias
2,
Cláudia Martins Carneiro1,3
, Angélica Alves Lima1,4
1Laboratório de Bioquímica Clínica. Programa de Pós-
graduação em Ciências Farmacêuticas UFOP – Ouro
Preto/MG 2 Laboratório de Bioquímica Clínica UFOP – Ouro
Preto/MG 3Laboratório de Imunopatologia/NUPEB. Departamento
de Análises Clínicas da Escola de Farmácia UFOP –
Ouro Preto/MG 4Laboratório de Bioquímica Clínica. Departamento de
Análises Clínicas da Escola de Farmácia UFOP – Ouro
Preto/MG
In Brazil, cervical cancer is the third most prevalent
neoplasia and the fourth leading cause of death in
women. The primary risk factor for cervical cancer is
the persistent infection with high-risk Human
Papillomavirus (HPV). In 2014, the HPV vaccine was
introduced in Brazil's National Vaccination Calendar for
female adolescents. After the implementation of the
vaccine in public health programs, several countries
have developed studies in order to identify local
particularities and improve service to the population.
The objective of this study was to analyze knowledge
and perception of adolescents and their mothers about
HPV and cervical cancer in Ouro Preto, MG. The study
was conducted in two Basic Health Units in Ouro Preto.
11
The interviews were applied to 86 adolescents aged 11
to 13 years old and 62 mothers. For the asessment of
knowledge and perception about HPV, cervical cancer
and the vaccine it was established a score (Very Good-
VG, Good-G, Fair-F and Poor-P) based on the number
of correct answers. In addition, for the survey of
difficulties and lack of knowledge about the subject, an
individualized analysis and a comparative analysis of
the interview questions were accomplished. In the
knowledge and perception analisys it was observed that
14% of adolescents (VG/G) and 56.4% of mothers
(VG/G) declared they were aware of the issue, but only
one adolescents (G) and 35.5 % of mothers (VG/G)
demonstrated a real knowledge about the subject. The
main difficulties observed were regarding to:
applicability of the HPV vaccine, number and frequency
of doses, prevention, signs and symptoms of HPV
infection and cervical cancer causes. The results of this
work has shown the low knowledge of the interviewers
about HPV, vaccine and cervical cancer. In addition,
most of adolescents and mothers believe they are more
updated on the issue addressed (perception) than they
actually are. This reinforces the need of carrying out
educational activities aimed to this population.
Acknowledge and financial support: UFOP,
Cipharma, Capes.
5
CARDIOTOXICITY ASSESSMENT OF FREE
ARTEMETHER CARRIED IN NANOCAPSULES.
Vidal-Diniz AT1, Silveira APA
1, Andrade RP
1, Souza
ACM1, Castro QJT
1, Guimarães HN
2, Richard S
3,
Mosqueira V.C.F1, Grabe-Guimarães A
1
1CiPharma, Escola de Farmácia, Ufop, Ouro Preto, MG;
2DEE, Escola de Engenharia, Ufmg, Belo Horizonte,
MG, Brazil;
3Universitè De Montpellier 1.
Malaria is caused by parasites Plasmodium. Tropical
and subtropical regions are the most affected and the
age group most targeted are children because of their
high susceptibility. The artemether (ATM) is the most
utilized treatment for Malaria, but it presents low oral
bioavailability, low elimination, half-life and toxicity,
mostly, in the central nervous system and cardiac.
Evaluated the cardiotoxicity in vivo of ATM, as well as
in its free form (ATM FREE) and encapsulated (ATM
NANO) form, in chronic treatment. The protocols used
were approved by CEUA UFOP under N° 2011/03.
Male Wistar rats were used, weighing between 220-
250g. To evaluate the cardiovascular parameters of
ECG: QT, RR, PR, QTc intervals and QRS complex,
and arterial pressure parameters (AP). The animals
were distributed in different experimental groups (n=8).
It was received the drug intravenously, at doses of 40,
80 and 120 mg/kg of ATM FREE and ATM NANO, and
as the control group the animals received vehicle and
empty nanocapsules (EN). after vehicle administration
and EN, there were no significant changes in baseline
parameters (BP) and ECG of the animals, the
administration of 120 mg/kg of ATM FREE caused
severe hypotension leading to discontinuation of
experiments with this dose. The administration of ATM
FREE (40 and 80 mg/kg) led to variations of 19% and
33% QT interval, significant reduction in heart rate (HR)
15% and 20% compared to baseline, while the ATM
NANO did not significantly alter the QT interval and HR.
The increase in QTc was reduced by 12%,
approximately, compared to the treatment with ATM
Free and ATM NANO. ATM FREE induced pronounced
decrease in AP and ATM NANO reduced hypotension.
There were no relevant changes in PR interval and
QRS complex. The results demonstrated that ATM
NANO reduced cardiotoxicity. ATM NANO maintained
its antimalarial activity and showed reduced
cardiotoxicity when compared to its free form,
suggesting a change in the drug distribution.
6
THE USE OF CINEMA IN BIOETHICS AND
HUMANISTIC EDUCATION IN PHARMACEUTICAL
SCIENCES; PROVOKING REFLECTIONS
Bruno de Resende Vieira1, Thayná Vichiato Silva
1,
Lidiane Rodrigues Antunes1, Marcela Torisu Lemos
1,
Catarina Stivali Teixeira1, Thiago Gouveia
1; Elza
Conceição De Oliveira Sebastião1.
1Escola de Farmácia, UFOP, Ouro Preto, Minas Gerais,
Brasil.
Audiovisual resources, especially the cinema, have
been widely used as facilitators in the teaching-learning
process. They play complementary role in training
healthcare professionals and provoke reflection on
bioethical and anthropological issues in order to
enhance the structure of the character of the individual.
The Brazilian National Curriculum Guidelines for
Graduation Schools highlight the important role of the
humanities in healthcare professionals training.
Pharmacy Courses, the students need the opportunity
to learn bioethical concepts throughout their
undergraduate training, in order to establish
professional-patient relations based on care and
respect, promoting autonomy and the preservation of
human dignity. To carry out practical alternative and
extracurricular activity, offered to Pharmacy academics,
to the promotion of creative and critical discussions of
relevant anthropological and clinical issues. The
12
development consisted in film screening followed by
discussion/debates. The movie collection was obtained
by donations of original copies of the personal
collection of the tutor of the project. The target
participants were all ongoing Pharmacy undergraduate
students of the Pharmacy School of Ouro Preto
University, in 2015. The promotion of film events was
made by printed posters and social networks like
Facebook, Twitter and the university website. The
participants of each event were invited to evaluate each
event by filling out anonymous and confidential
questionnaires. Seventeen movies were shown from
September/2014 to August/2015 to an audience of 226
participants; The subliminal themes of the movies were
classified as ethics in science, organ donation,
overcoming, psychiatric disorders,
spirituality/faith/belief, sexual orientation, drug use,
alcoholism, human relations, feminism, violence against
women and general discrimination. There was an
average of 13.3 viewers/film and the average time of
debate of 40 minutes; Event was positively reviewed by
96% of viewers. Selected film screenings followed by
respective debates allowed the participants to discuss
taboo themes of social, cultural, psychological and
medical-scientific aspects. It has emerged as an
effective alternative because it recreates situations
close to reality, sparking participant’s feelings capable
of producing reflections on moral and bioethical
dilemmas that can be used in problem-solving of real
life. This project permitted the expansion of the
participants horizons about the topics discussed and
integrated approach between professional practice and
ethics, stimulating their critical evaluation and effective
understanding of patients' unique narratives and needs.
It must be used as a significant teaching tool for
achieving humanistic educational objectives in the
undergraduate Pharmacy curriculum.
Financial support and thanks to: PROGRAD-UFOP e
GEPhar.
7
PHARMACOLOGICAL TREATMENT (NON)
ADHERENCE AMONG WOMEN
DIAGNOSED WITH BREAST CANCER LIVING IN
OURO PRETO
Catarina Stivali Teixeira1, Elza Conceição de Oliveira
Sebastião2, Vanja Maria Veloso
2
1CIPHARMA, Escola de Farmácia, UFOP;
2DEFAR - Escola de Farmácia – UFOP;
Introduction: Although incidence and mortality rates
for women living with breast cancer (WLBC) have
increased in recent years, this type of cancer has a very
good prognosis if diagnosed and treated properly. It is
known that pharmacological treatment (PT)
(non)adherence isinterrelated to factors associated with
the patient, with the treatment itself and with the health
services. Adhering to the PT reduces morbidity and
mortality of cancers as well as the costs of healthcare.
The psycho-socio-economic aspects should not be
neglected, since PT non-compliance possible causes
failure of chemotherapy of WLBC. Objective and
Methodology: This study aims to give insight into the
process of (non-)adherence by researching influencing
factors in breast women cancer taking PT by means of
a qualitative study with semi-structured interviews. The
sample will consist of 30 participants treated with PT.
The consent by the coordinator of the group was
obtained and the research was approved by the
UFOP´s Ethics Committee under the registration
49488415.0.0000.5150. Potential results: At this
moment, there are no results because the data
collection is programmed to be conducted at 2016.
However, there are expectations regarding the impact
of PT, that social support from family and friends, and
recognition from healthcare professionals are expected
to influence the process of (non-)adherence . The
results of this study can help healthcare professionals
understand why WLBC under PT do not always adhere
to taking the therapy and may facilitate patient-tailored
interventions at the specific support group at Ouro
Preto.
Keywords: adherence; Pharmacological therapy;
influencing factors; qualitative study, Pharmaceutical
care.
Acknowledgements: PROPP/UFOP and CIPHARMA
for financial support. To AMBAR and GEPHAR groups
by scientific support.
Financial support: PROPP/ UFOP
8
TOXICOGENETIC EFFECTS OF SILIBININ IN
BLADDER CANCER CELL LINES
Daiane Teixeira de Oliveira¹, André Luiz Ventura
Sávio², João Paulo de Castro Marcondes², Glenda
Nicioli da Silva¹.
1Laboratório de Pesquisas Clínicas /CIPHARMA /
Universidade Federal de Ouro Preto (UFOP) - Ouro
Preto/MG
²Laboratório de Toxicogenômica e Nutrigenômica
/Universidade Estadual Paulista (UNESP)-Botucatu/SP
e-mail: [email protected]
Silibinin is a natural phenol derived from the
Flavonolignan family and founded in seeds of wild
13
artichoke plant or thistle. Recent data have shown its
effectiveness in preventing/treating bladder tumors in
vivo, making it a promising antineoplastic agent. TP53
mutations are the most common alterations in bladder
cancer cells, and are related to treatment response.
Thereby, in this study we investigated the mechanism
of action of silibinin in bladder cancer cells with different
TP53 gene status. Two bladder urothelial carcinoma
cell (UCC) lines were used: RT4, with wild-type TP53
gene; and T24, with mutated TP53 gene. Silibinin was
tested at concentrations of 50, 75, 100, 115, 130, 135,
150, 200 and 250 µM in cell proliferation and
clonogenic survival assays, cell cycle kinetics,
apoptosis rates and comet assay. The data showed
increased primary DNA damage levels in both cell lines.
Furthermore, the results demonstrated decreased cell
proliferation rates in the TP53 mutated cells, but not in
TP53 wild type cells. However, the clonogenic survival
assay revealed significant decreases in RT4 and T24
cell colonies. Increased early apoptosis rates were
observed in both cell lines, while late apoptosis rates
were only detected in T24 cells. No changes in cell
cycle kinetics were observed. In conclusion, silibinin
promoted decreased proliferative capacity in cell lines
of bladder urothelial carcinoma possibly by inducing
DNA damage and activation of cell death independent
TP53 status. These results provide data that can help in
the elucidation faction mechanism of action of silibinin
in the treatment of bladder cancer.
Keywords: Silibinin, Bladder Cancer, Gene TP53,
Comet assay, Cell cycle, Apoptosis, Cytotoxicity.
Acknowledgment: Dra. Daisy Maria Fávero Salvadori,
Me. André Luiz Ventura Sávio, Dr. Alexandre Reis
Barbosa, Dra. Cláudia Martins Carneiro, Dra. Marta de
Lana, UFOP, CiPharma, UNESP-Botucatu, Laboratório
de Imunopatologia-Nupeb, Laboratório de doença de
Chagas-Nubep.
Funding: FAPEMIG, CNPQ, CAPES, UFOP.
9
EVALUATION OF CARDIOTOXICITY OF
CASPOFUNGIN IN VIVO
Paula, DCC1; Garcia, IC
2; Guimarães, HN
3; Grabe-
Guimarães, A2.
1Programa de Pós graduação em Ciências
Farmacêuticas – CiPharma; 2Departamento de
Farmácia da UFOP; 3Departamento de Engenharia
Elétrica da UFMG.
The incidence of fungal infections has increased
considerably. The resistance to conventional antifungal
agents and their toxicity are some factors that suggest
new drug delivery systems development to treat
systemic fungal infections. Objective: To evaluate the
cardiac toxicity of intravenous (IV) caspofungin by the
electrocardiogram signal (ECG) Methods: The ECG
was obtained in vivo in healthy female Swiss mice
treated with free caspofungin 5 mg/kg/IV, once daily
during 7 days (n = 8). The animals were anesthetized
with ketamine/xylazine and ECG signals were obtained:
before treatment, 15 and 21 days after the end of
treatment. At 30 days after the end of treatment,
animals were challenged with IV noradrenaline (NA) 1,
3 and 10 µg/kg. A control group without treatment
received only NA (n = 8). All procedures were
previously approved by the Ethics Committee on
Animal Use UFOP under no. 2014/37. Results: The
multiple doses administration of caspofungin induced a
significant increase of ECG QT interval (10% ± 4.1) at
21 days after last administration. NA induced extra-
ventricular arrhythmias, indicating an arrhythmogenic
potential of free caspofungin. Conclusion: Preliminary
results confirm caspofungin cardiotoxicity already
reported in heart and isolated rat cardiomyocytes. We
expect that the encapsulation of caspofungin in solid
lipid nanoparticles (SLN), the cardiotoxic effects will be
eliminated, and it could be safier to treat fungal
infections.
Financial supports: FAPEMIG (Rede Nanobiomg,
CDS-PPM - 2013), UFOP
10
METHOD DEVELOPMENT BY HPLC FOR
QUANTIFICATION OF CANDESARTAN CILEXETIL
IN BIOPHARMACEUTICAL STUDIES
Campos, D.P. 1, Barcellos, N.M.S.
1, Souza, J
1.
1Universidade Federal de Ouro Preto, Laboratório De
Controle de Qualidade - Ouro Preto, MG, Brasil
Biopharmaceutical studies consist in the determination
of solubility and permeability of a drug and dissolution
from the dosage form. These studies will allow the
allocation of candesartan cilexetil, an antihypertensive
classified as antagonist of angiotensin II receptors
(AT1), in the Biopharmaceutical Classification System,
contributing to a safe decision about the possibility of
bioiwaiver for new products and increasing the supply
of available drugs by the program “Farmácia Popular do
Brasil”. Before performing biopharmaceutical studies it
is necessary to develop an analytical method suitable
for quantification of the drug without interference from
the matrix or the possible degradation products. This
preliminary study aims to demonstrate how the method
for quantification of candesartan cilexetil is being
14
developed. Starting with the identification of the
analytical conditions described in the literature: 1)
Sodium phosphate monobasic buffer, acetonitrile
(50:50), pH 3; flow 1 mL/min; detection photodiode
arrays (DAD)-210nm; CN column (250 x 4.6 mm, 5μm);
Temperature (T) 27°C. 2) 0.05 mol/L Potassium
phosphate monobasic buffer pH 3.0, acetonitrile,
methanol (30:50:20); flow not indicated; detector UV-
visible-215nm; C18 column (250 x 4 mm, 5μm);T not
indicated. 3) Methanol, acetonitrile, 10 mmol potassium
phosphate monobasic pH 4.0 (90:8:2); flow 0.8 mL/min;
detection at 254nm; column not indicated; T not
indicated. 4) Acetonitrile, water, acetic acid 90: 10: 1
and 57: 43: 1 (gradient elution) pH not indicated; flow
0.8 mL/min; detector UV-visible-254nm, C18 column
(150 x 4 mm, 4μm);T 25°C. 5) Acetonitrile, 5 mM
ammonium acetate pH 2.5 (47:53); flow 1.5 mL/min;
mass detector; C18 column (50 x 4.6 mm, 3.5μm); T
20°C. The conditions were tested on C18 column (150
x 4mm, 3,5μm); DAD-254nm; T 25°C. 1) It was not
tested because it showed instability with the media in
which the drug will be quantified; 2) Presented retention
time (rt) 38 minutes; 3) presented rt: 4 minutes; 4) The
two conditions were tested alone in isocratic elution, the
first rt was 3 minutes, very close to the void time and
the second around 18 minutes; 2) and 4) were not
adequate for the required time of analysis; 3) Have a
suitable rt but there was no separation of degradation
products and is therefore not selective, so 5) will be
tested. The method should be developed in accordance
with the required analytical application and it is a
process that aims a smaller rt, higher sensitivity and
symmetry of the chromatographic signal without
interference constituents of matrix or degradation
products.
Acknowledgements: UFOP/CiPharma/Ministério da
Saúde/Anvisa/FAPEMIG/Rede Toxifar/CNPq.
Keywords: Method development, Candesartan
cilexetil, Biopharmaceutics.
11
TOXICOLOGICAL EVALUATION OF THE
ACCELERATED CLEARANCE BLOOD
PHENOMENON FOR THE LIPOSSOMAL
TREATMENT IN LEISHMANIA INFANTUM INFECTED
MICE
Débora Faria Silva¹; Tamara Cristina Moreira Lopes¹;
Walyson Coelho Costa¹;
Adriel Araújo Fernandes
Ferreira2; Neila Márcia Silva Barcellos¹; Rodrigo Dian
De Oliveira Aguiar Soares3; José Mario Barichello¹;
1Simone Aparecida Rezende
1
¹Programa de Pós-graduação em Ciências
Farmacêuticas (CiPharma) - Escola de Farmácia –
Universidade Federal de Ouro Preto (UFOP) 2Departamento de Fisiologia e Biofísica – Instituto de
Ciências Biológicas (ICB) – Universidade Federal de
Minas Gerais (UFMG) 3Núcleo de Pesquisa em Ciências Biológicas (NUPEB)
– Universidade Federal de Ouro Preto (UFOP)
Introduction: The World Health Organization
encourages the study of new drugs or alternative forms
of administration for the treatment of visceral
leishmaniasis, among them the liposomal formulations.
These formulations could lead to reuse of tartar emetic
(TE), a potent anti Leishmania drug with limited use due
to serious toxic effects. Objectives: This study
evaluated the effect of the accelerated blood clearance
(ABC) phenomenon in Leishmania infantum infected
BALB/c mice treated with pegylated liposomes
containing TE. This effect is responsible by the rapid
clearance of a second dose of pegylated liposomes
from the circulation within a certain interval from
injection of the first dose due to the removal by
macrophages. Methods: conventional and pegylated
liposomal formulations were prepared by the hydration
of a thin film of lipids with a solution of the drug. The
effect of TE liposomal formulations on nitric oxide
production by LPS/IFN-gama stimulated J774-A1 cells
was evaluated by Griess method. In order to evaluate
the effect of ABC phenomenon on BALB/c treatment
with TE pegylated liposomes, mice were initially treated
with empty pegylated liposomes. One week later, they
were treated with pegylated liposomes containing 12
mg SbIII/kg. Parasite load was evaluated by limiting
dilution analysis. The effect of infection and treatment
was evaluated by analysis of hematological and
biochemical parameters. Results: TE liposomal
formulations had no effect on nitric oxide production for
J774-A1 cells. Pegylated liposomal formulation of TE
significantly reduced the parasite load in liver, spleen
and bone marrow of L. infantum treated mice. However,
ABC phenomenon had no effect on treatment with
pegylated formulation of TE. Free drug and
conventional formulation was able to reduce parasite
load only in liver. No impairment in the hepatic and
renal functions and hematological parameters was
observed due to the infection or treatment.
Conclusion: Pegylated liposomes containing TE were
more effective in reducing parasite load than
conventional liposomes. ABC phenomenon had no
effect on treatment with pegylated liposomes.
Financial support: CNPq, UFOP.
15
12
ASSESSMENT OF ANTI-HYPERURICEMIC ACTIVITY
AND INHIBITION OF HEPATIC XANTHINE OXIDASE
OF PIMENTA PSEUDOCARYOPHYLLUS AQUEOUS
EXTRACTS
Fernanda Cristina Ferrari1, Zilma Schimith Ferraz
Filha1, Camila Helena Barros
1, Marcus Antônio Ferreira
Santos1, Dênia Antunes Saúde-Guimarães
1.
1Laboratório de Plantas Medicinais, Escola de
Farmácia da Universidade Federal de Ouro Preto,
Brazil.
Gout is an acute inflammatory disease caused by the
deposition of urate crystals in the joints. Pimenta
pseudocaryophyllus (Gomes) Landrum (Myrtaceae) is
known as craveiro or cataia, being used by some
people as sedative, aphrodisiac, diuretic and against
arthritism. This study evaluated the anti-hyperuricemic
activity and inhibition of hepatic xanthine oxidase of
aqueous extracts of the species. A model of
hyperuricemia induced by potassium oxonate in Swiss
mice was used. The extracts were prepared using 70 g
of powdered leaves and branches, extracted with 1 L of
water each. After lyophilization 9.61 g of dried aqueous
extract of the leaves (EAF) and 4.86 g of dry aqueous
extract of the branches (EAG) were obtained. The
hyperuricemic animals that received EAF (125 mg/kg)
and EAG (500 mg/kg) showed statistically lower serum
uric acid compared with hyperuricemic group. The
extracts EAF (125 mg/kg) and EAG (500 mg/kg) were
capable to inhibit the hepatic xanthine oxidase enzyme
in 22.77% and 11.17%, respectively. Allopurinol (10
mg/kg) was able to reduce this enzyme in 82.06%.
Studies showed that the aqueous extracts were able to
reduce serum levels of uric acid. The mechanism of
hipouricemic action of EAF was via inhibition of hepatic
xanthine oxidase. Further studies are needed to
elucidate the anti-hiperuricemic mechanism of action of
EAG. The aqueous extracts of leaves and branches P.
pseudocaryophyllus have potential for use in the
treatment of hyperuricemia and gout.
Keywords: Pimenta pseudocaryophyllus;
Hyperuricemia; Xanthine oxidase; Inflammation, Gout.
Financial support: FAPEMIG CDS APQ-00956-13,
REDE TOXIFAR/FAPEMIG, PROBIC/FAPEMIG,
CAPES, UFOP.
13
ROLE OF IPRIFLAVONE INCORPORATION INTO
SELF-EMULSIFYING DRUG DELIVERY SYSTEMS
ON THE SEXUAL AROUSAL RESPONSE IN
FEMALE WISTAR RATS
LOPES IM1, MENDES JC
1, RODOVALHO GV
1, LEITE
R1.
1CiPharma, Pharmacy School, Federal University of
Ouro Preto, Brazil.
Introduction: The menopausal hypoestrogenism is
related to symptoms of sexual dysfunction such as
decreased sexual arousal and difficulty in achieving
orgasm. Estrogen replacement therapy (ERT) is an
effective treatment, but it is associated with
predisposition to breast and endometrium cancer.
Objective: In this study, we evaluated the effect of the
fitoestrogen ipriflavone incorporation into self-
emulsifying drug delivery systems (SEDDS) as an
alternative to ERT on the female sexual function.
Methodology: We evaluated the sexual arousal
response of adult female Wistar rats with regular
ovulatory cycle (OVR), ovariectomized (OVX),
ovariectomized treated with estradiol cypionate (OVE,
38 µg/kg, subcutaneous) or iprifavone (OVI, 10 and
30mg/kg,) for 7 days. Sexual arousal response was
stimulated by apomorphine (80 µg/kg, subcutaneous), a
dopamine agonist, which causes genital grooming
response associated with temporary clitoris
engorgement. After apomorphine or ascorbic acid
(control) administration, animals were placed in a box
with front and bottom transparent sides and videotaped
for 30 minutes. The number of grooming, during this
period, was considered an indicator of arousal
response. Results: OVR rats had an increased arousal
response induced by apomorphine when compared to
the control (ascorbic acid administration in OVR).
Estrogen replacement was able to restore reduced
arousal response caused by ovariectomy. There was
no apomorphine-induced arousal response in OVI
group, this response was lower than control OVI group
that receive only administration ascorbic acid. The lack
of response stimulated by apomorphine in OVI group
may be related to the preparation or degradation of
apomorphine. Conclusion: It is necessary additional
experiments to conclude the effects of ipriflavone in
facilitating the sexual arousal in female rats.
Key words: hypoestrogenism, fitoestrogen, sexual
arousal, female rats.
Acknowledgements: This work was supported by
Conselho Nacional de Desenvolvimento Científico e
Tecnológico (CNPq) and Fundação de Amparo à
Pesquisa do Estado de Minas Gerais (FAPEMIG).
14
PREVENTIVE EVALUATIONS AND LAB TEST ON
WOMEN AT THE CLIMACTERIC
16
Sousa, J.C.1; Ribeiro, M.M.P.F
1; Faria, A.P.C.
1;
Gouvea, T.M.1; Gaede-Carrillo, M.R.G.
1; Lima, A.A.
1;
Veloso, V.M.1
1Escola de Farmácia, UFOP,
, Ouro Preto, MG
Although the climacteric is a natual and physiological
process, part of women's aging, is too complex,
generating events on a female body, causing
physical,psychic,metabolic and behavioral changes.
Among the metabolic changes in this stage, there are
many factors that increase the risk for osteoporosis and
for cardiovascular diseases, which is the main cause for
post menopausal women's death. The goal of this
project was to measure the knowledge and to check out
if there was any risk factors for osteoporosis and
cardiovascular diseases in the women at the
climacteric. There were 53 evaluated women with the
age between 45 and 60 years old in four health centers.
There was a interview using questionnaire that was
divided into three parts. The first one addressed the
demographic and health profile, the second one had
questions according to the the knowledge of risk factors
for cardiovascular diseases (CVD) and the third one
checked out if there was any risk factors for
osteoporosis diseases. Body measurements and
laboratory evaluations were made with the participants.
The average age for women was 53 years old (± 4,2).
The changed lipid profile was found in 73,6% of the
women. The most risk factors were: 94,1%
changed waist circumference (WC), 81,1% obesity
or overweight. The most part of the women knew that
aspects like: age, obesity, smoking, sedentary
lifestyle, diabetes mellitus, alcoholism and stress, are
the important risk factors for cardiovascular diseases.
Even if they know it 69,8% keep sedentary and 22,6%
keep smoking. All the women did show at least one risk
factor for osteoporosis. We can conclude that even the
knowledge and changed risk factors for CVD it is not
enough to motivate changes in the lifestyle. The results
show the importance and necessity of information
programs, specially proposing changes that motivate
changing behavior.
Financial support: PROEX - Pró-reitoria de Extensão
Universidade Federal de Ouro Preto, FAPEMIG -
Fundação de Amparo a Pesquisa de Minas Gerais
15
ROLE OF THE NO/cGMP PATHWAY IN TISSUES
INCUBATED WITH DAF-FM OF YOUNG AND
SENESCENT FEMALE SHR RATS.
MENDES J.C1, MARTINS T. A.
1, RODOVALHO G. V.
1,
LEITE R.1
1CiPharma, Escola de Farmácia, UFOP, Ouro Preto,
MG;
The hypoestrogenism common in menopause can lead
to potentially pathological and universal manifestation
consequences, such as sexual dysfunction, directly
affecting the life quality of women. The involvement of
estrogens in the female genitalia function appears to be
mediated by nitric oxide, the expression of nitric oxide
synthase (nNOS) regulated by estrogen levels. The
cellular distribution of NOS in the vagina of female rats
suggests that nitric oxide (NO) can modulate both the
blood supply to the mucosa as for vaginal smooth
muscle. This study aimed to qualitatively analyze the
presence of NO in cells of vaginal tissues by
fluorescence technique, using reagent diamine
diacetate (DAF-FM) which interacts with the NO
molecule present in tissues, forming a fluorescent
compound. The vaginal tissue were incubated with
DAF-FM (2,5 µM, 1 µM and 0,5 µM) associated with 5
µM of acetylcholine for 10 minutes at room
temperature. Then the tissues were washed in PBS
(three times for 5 min) and incorporated into "tissue tek”
and frozen in -80°C overnight. Adjacent sections (10
µM) were then prepared with the aid of a cryostat.
Tissues were mounted in special blades (chrome-
alum), air dried, and covered with glycerol/tris mounting
medium. Subsequently, the slides were analyzed by
fluorescence microscopy (488 nm).
Our results confirm that incubation of vaginal tissue with
DAF-FM allow detection of NO produced locally at
basal conditions and after stimulantion with
acetylcholine.
Financial support: FAPEMIG, CAPES and UFOP.
16
LC/MS PROFILING OF XANTHONES AND
FLAVONOIDS OF THE ANTI-DENGUE EXTRACTS
AND CONSTITUENTS OF ARRABIDAEA
SAMYDOIDES (BIGNONIACEAE)
Fonseca, J.M.1; Silva, B.M.
2; Kroon, E.G.
3; Oliveira,
A.B.4; Brandão, G.C.
1,
1Universidade Federal de Ouro Preto - Departamento
de Farmácia;
2Universidade Federal de Ouro Preto - Departamento
de Ciências Biológicas;
3Universidade Federal de Minas Gerais - Microbiologia;
4Universidade Federal de Minas Gerais - Departamento
de Produtos Farmacêuticos;
Introduction: Arrabidaea samydoides (Cham.)
Sandwith belongs to the Bignoniaceae family that is
17
represented by more than 100 genera and about 800
species. Chemically, bignoniaceous plants are
characterized by the presence of flavonoids,
terpenoids, quinones, mainly naphthoquinones, and
aromatic compounds such as lignans, cinnamoyl,
benzoyl and acetophenone derivatives. Several species
of this family are reported as used for treatment of
diseases possibly related to viral infections which
motivated the evaluation of A. samydoides [1].
Methods: The in vitro antiviral activity against Dengue
virus 2 (DENV-2) was carried out by the MTT
colorimetric method (n =4); the cytotoxicity was
determined in vitro in LLCMK2 cell lines (n=4);
interferon was used as positive controls (2.5×103 UI/ml)
[2,3]. Ethanol extracts from stems, and leaves were
prepared and submitted to analyzsis by TLC and RP-
UPLC-DAD-MS using previously validated methodology
[1]. The isolation of constituents was done using silica
gel chromatography column, Sephadex LH20 and High-
performance liquid chromatography for preparative
scale. Results: Results are presented as cytotoxic
concentration to 50% (CC50), antiviral effective
concentration to 50% (EC50) and Selectivity Index (SI)
values. The extracts presented low cytotoxicity (CC50 >
90 g/ml). All ethanol extracts were active against
DENV-2, EC50 < 25.0 g/ml and SI > 3.0. UPLC-MS
analyses of extracts, allowed the identification of the
majority of flavonoids and xanthones present in those
extracts. The flavonoids have been identified: as
vitexin, isovitexin, orientin, isoorientin, chrysin, O-
methylchrysin, and xanthones as mangiferin,
isomangiferin, 2’-trans-O-caffeoylmangiferin, 2’-trans-O-
coumaroylmangiferin, 2’-trans-O-cinnamoylmangiferin,
muraxanthone, and 2’-O-benzoylmangiferin. The
chromatographic fractionation leads to the isolation of
three flavonoids called AS1, AS2 and AS3. AS1, AS2
and AS3 were identified as vitexin, isovitexin and
orientin, respectively. AS2 and AS3 showed antiviral
activity with EC50 of 79.3 ± 3.2 and 85.6 ± 4.8,
respectively. AS1 showed no antiviral activity.
Conclusions: The results of the phytochemical studies
here described suggest that flavonoids and xanthones
are the substances that contribute to the antiviral
activity of the ethanol extract of this species.
Financial support: FAPEMIG and CNPq.
17
IN VITRO EVALUATION OF CYTOTOXICITY
NANOSTRUCTURED SYSTEMS CONTAINING
DRUGS ANTI-TRYPANOSOMA CRUZI
de Castro, Kelly Christyne Miranda Pereira1; Mazzeti,
Ana Lia1; Trindade, Izabel Cristina
1; Mosqueira,
Vanessa Carla1; Bahia, Maria Terezinha
1.
1Laboratório de Doenças Parasitárias - Escola de
Medicina – CiPharma - Universidade Federal de Ouro
Preto
Current specific anti-T. cruzi chemotherapies are
unsatisfactory. These therapies are based on
nitroimidazoles or nitrofurans agents such as
benznidazole (Rochagan®, Rodanil®, Roche) and
nifurtimox (Lampit®, Bayer) that were developed
empirically three decades ago. The major limitation of
these compounds is their lower anti-parasitic activity in
the chronic phase of the disease, as 80% to 100% of
treated patients are not cured. Recently newer
strategies based on reformulation have been used to
improve the efficacy of old drugs lacking adequate
pharmacokinetic and biodistribution profile. There has
been a resurgence of interest in nanoemulsions for
various pharmaceutical applications since low-energy
emulsification methods, such as spontaneous or self-
nanoemulsification, have been described. Self-
nanoemulsifying drug delivery systems (SNEDDS) are
anhydrous homogenous liquid mixtures consisting of
oil, surfactant, drug and coemulsifier or solubilizer,
which spontaneously form oil-in-water nanoemulsion
improving the drug delivery. In vitro and in vivo models
are fundamental tools for the evaluation of the
therapeutic efficacy of antimicrobial agents. There are
well established intracellular and extracellular screens
for in vitro hit identification and rodent models for in vivo
screening and evaluation of anti-T.cruzi drug activities.
But studies using these models have not addressed
their predictive value. More specifically, there has been
no analysis linking data in vitro and in vivo studies in
order to gain an understanding of the predictability of in
vitro models used in drug development. The aim of the
present study is to analyze the toxicity and anti-T. cruzi
activity of nifurtimox- and ravuconazole-SNEDDS in
vitro providing a basis for refined drug lead
optimization. The toxicity and anti-T.cruzi activity of the
nifurtimox- and ravuconazole-SNEDDS, of each of its
components alone and of the free compound were
evaluated using the H9c2 rat cardiomyoblast and
HepG2 cell lines as experimental model. Our
preliminary results indicate that both, nifurtimox- and
ravuconazole-SNEDDS, have an increased activity
against the amastigote forms of T. cruzi, compared to
free drugs. However, this formulation had no effect in
the toxicity reduction for mammal host cells.
Supported by: CNPq, Fapemig e UFOP.
18
SOLUBILITY IN EQUILIBRIUM AMILORIDE
HYDROCHLORIDE BY ORBITAL SHAKE FLASK
METHOD: A PILOT STUDY.
18
Laryssa Freitas Guimarães1, Jacqueline de Souza
1,
Bruna Pegorelli1 e Neila Márcia Silva-Barcellos
1.
1Curso de Pós-graduação em Ciências Farmacêuticas -
CiPharma - Universidade Federal de Ouro Preto, Ouro
Preto, Minas Gerais, Brasil.
The drug solubility, permeability and dissolution are
determinant factors in their absorption process. The
drug solubility can be studied and determined by
various methods, among them the orbital shake- flask.
This work has as one of its objectives the solubility
equilibrium study by the shake-flask method of the
amiloride hydrochloride, a potassium-sparing diuretic
used for treating hypertension. To this end, is being
developed an analytical HPLC method aiming at the
amiloride hydrochloride quantification in buffered media
pH = 1.2; 4.5 and 6.8. The chromatographic conditions
were initially evaluated: photo diode detector, C18
column (3.5µm x 4,6 x 150 mm) at 25 ° C, mobile phase
composed of 10% acetonitrile and 90% acidified water
acetic acid (pH adjusted to 4), flow 1.0 ml/min. The
chromatograms were shown to be adequate for
quantification of amiloride hydrochloride and have
allowed their identification in 4 min. A pilot study of the
solubility has evaluated as amount of initial raw material
of excess drug 7.09 mg, 42.47 mg and 2.12 mg in
buffer pH 1.2 ; 4.5 and 6.8, respectively. The samples
were continuously agitated in apparatus orbital shaker-
flask (Incubator Shaker IKA) for 24 hours. The results
showed that the amiloride hydrochloride solubility in
medium of pH 1.2 was 706.05 µg/mL related and
established after 2 hours and 30 minutes from the start
of the experiment. For the medium of pH 4.5 the
solubility was 3949.65 µg/mL after 4 hours of
experiment. Already in the medium of pH 6.8, the
solubility was 226.01 µg/mL after 4 hours of the onset
of the experiment. It can be concluded that the solubility
shake-flask method is able to estimate the solubility of
the drug as well as the analytical method development
is shown to be suitable for their quantification.
Acknowledgments: UFOP; FAPEMIG; CAPES for
their support.
19
POSSIBLE INTERACTION BETWEEN
CANDESARTAN AND THE PHYTOESTROGEN
"IPRIFLAVONE" ON FEMALE SEXUAL FUNCTION
OF HYPERTENSIVE (SHR) AND NORMOTENSIVE
(WKY) RATS
Leidiane V. SIMÕES, Thales A. MARTINS, Vanessa C.
F. MOSQUEIRA, Andrea G. GUIMARÃES, Romulo
LEITE.
Sexual dysfunction is characterized by disturbances
and psychophysiological changes in sexual response
cycle (desire, arousal, orgasm and resolution). Factors
such as age, hypertension, smoking,
hypercholesterolemia and pelvic surgeries interfere in
men's and women's sexual function. It is known that
drugs used for the treatment of hypertension also
interfere on the sexual response, however, studies
have shown that the angiotensin II receptor antagonists
(ARAs), effective in controlling blood pressure,
apparently do not cause, or even mitigate, sexual
dysfunctions. The beneficial effect of ARAs was
observed in studies performed on experimental models
of erectile dysfunction in male rats. In this context, it is
relevant to assess the therapeutic potential of the
combination of an antihypertensive agent with a
substance capable of improving the sexual response in
hypertensive female rats. A strong candidate to be
studied is a phytoestrogen called ipriflavone, synthetic
derivative of daidzein (isoflavones), whose chemical
structure is similar to estradiol and can bind to estrogen
receptors simulating a similar effect of the hormone.
Ipriflavone is already used in the prevention and
treatment of osteoporosis and studies have
demonstrated its high potential to minimize the
symptoms of menopause. Therefore, our goal is to
study the therapeutic potential of candesartan, of
ipriflavone, and the combination of both in the
regulation of sexual function in hypertensive young rats
(SHR) and normotensive (WKY). Young hypertensive
rats (SHR) and normotensive rats (WKY) (with 4 and 5
months) will receive treatment for 30 days, periodically
monitoring the estrous cycle, blood pressure and
vasocongestive response induced by apomorphine. At
the end of treatment changes in temperature,
lubrication and intravaginal pressure induced by pelvic
nerve stimulation will be evaluated. It will be determined
the expression of isoforms of nitric oxide neuronal
enzyme (nNOS) and endothelial (eNOS) by Western
Blotting technique and the reactivity of vaginal tissue
strips will be evaluated in a system for isolated organs.
Nitric oxide and superoxide anion detection in isolated
vaginal tissue will be performed by means of
fluorescence techniques. It is expected that both
candesartan as ipriflavone, as well as the combination
of those substances, improve sexual function in young
female hypertensive rats (SHR) with responses similar
to those observed for female normotensive rats (WKY).
Acknowledgements: CiPharma. Financial Suport:
CNPq, FAPEMIG, CAPES e UFOP.
19
20
EXTENSION PROJECT OF HEALTHCARE
COMMUNITY AGENTS EDUCATION ON
PREGNANCY AND MOTHERHOOD AT TWO BASIC
HEALTH UNITIES FROM OURO PRETO IN 2015.
Lidiane Rodrigues Antunes1; Thayná Vichiato Silva
1,
Bruno de Rezende Vieira1, Marcela Torisu Lemos
1,
Catarina Stivali Teixeira2, Thiago Gouveia
2; Elza
Conceição de Oliveira Sebastião1
1Escola de Farmácia/UFOP, Ouro Preto, Minas Gerais,
Brasil, 2CIPHARMA, Escola de Farmácia, UFOP, Ouro Preto,
Minas Gerais, Brasil;
Pregnancy brings many changes and expectations for
women and their families, since it demands new
emotional, social, and legal responsibilities that go
along with motherhood. The health of pregnant women
and prenatal care are important and current topics that
aim to reduce morbidity and mortality of both mothers
and newborns and improve their quality of life. In public
healthcare services in Brazil, the Healthcare
Community Agents (HCA) are responsible for educating
families and their job must be based on the reality of
each one, considering individuals in their particularity,
complexity and completeness, as well as socio-cultural
integration. To carry out this role, it is essential that
these agents have knowledge and proper information.
This extension project intended to prepare HCA by an
educational approach to pregnant women, both
prenatally and at the postpartum period. Teaching
strategies of lectures, group dynamics, display
instructional videos and practical classes were used at
the classes for 11 HCA of two Basic Health Units, in
Ouro Preto at 2015. It was prepared an auxiliary digital
material after compiling scientific available information
containing training information and its references and
sources. Highlights: General care during pregnancy,
childbirth, complicated conditions, breastfeeding, care
of the newborn, drugs in pregnancy and breastfeeding.
The project was developed through the participative
methodology techniques. It has provided 5 day-events
of 20 hours total of learning and experience
opportunities to the CHA in the field of health education
with pregnant women; providing the elaboration of
teaching technologies that were used to guide the
Agents in the theme; undertake educational activities
among students; develop research projects among the
students that will revert to new teaching, intervention,
and knowledge generation practices. The participants
responded to a questionnaire which showed high
suitability of topics, perceived importance and
satisfaction with the implemented project. The didactical
techniques involved the experience of different social
and psychological realities, represented a space for
reflection about pregnancy and motherhood, enhancing
the HCA knowledge’s about the basic care including
prevention, health promotion and treatment of problems
that can occur during that particular period and after
delivery. The training course was a rich source of
exchange of information, experience and gaining
knowledge about the subject matter. The workshops
were effective tools in the education on prevention and
on the promotion of pregnant/babies healthcare, and
may be carried out in the health services by
interdisciplinary teams.
Financial support: PROEX/UFOP. Thanks:
PROEX/UFOP.
21
DEVELOPMENT OF THE ANALYTICAL METHOD
AND PROSPECTS OF CHEMOMETRICS AIMING
BIOPHARMACEUTICAL STUDIES
Lorena Barbosa Arrunátegui*1; Dâmaris Laignier
Rodrigues de Lacerda2 ; Lara Maria Lopes de Castro
1;
Bruna de Carvalho Mapa1; Neila Márcia Silva
Barcellos1,2
; Jacqueline de Souza1,2
.
1Programa de Pós-graduação em Ciências
Farmacêuticas (CiPharma) - Universidade Federal de
Ouro Preto; 2Escola de Farmácia - Universidade Federal de Ouro
Preto;
Introduction: To infer the biopharmaceutical
classification of drugs is necessary to promote
dissolution and solubility experiments that should be
executed with the use of properly developed and
validated analytical methods. The chemometrics is
intended for analysis of chemical data of multivariate
nature and collaborates to the development. Thus, the
first step of a biopharmaceutical evaluation is to
develop a reliable quantification method. Objective:
Identify favorable chromatographic conditions for
quantification of desloratadine and loratadine for
subsequent application of chemometrics and validation,
so obtaining a reliable method to start the
biopharmaceutical studies. Methods: The method was
developed in chromatograph Waters and DAD detector.
Samples were prepared containing one or both drugs.
Different conditions for the quantification of the drugs
were performed. The components of mobile phase
tested were: ultra purified water, acetonitrile, methanol,
phosphate buffer, 0.1% acetic acid in water. Column
temperature: 25°C and 30°C. Wavelengths: 247 nm
20
and 254 nm. Flow: 0.8 to 1.2 ml / min. The injection
volume: 20 to 60 µl. Columns: (1) C18 Waters
Simmetry® (150 x 4,6 mm, 5 µm); (2) C18 Varian® (50
x 4,6 mm, 5 µm); (3) C18 Waters SunFire® (150 x 4,6,
3,5 µm); (4) C18 Macherey-Nagel® (150 x 4,6 mm, 5
µm). When the condition that offered more satisfactory
selectivity, efficiency, symmetry and retention time was
found, it was evaluated purity of peak in the Empower
software. Results: It was noted that the peaks that
seemed satisfactory initially began to exhibit unusual
aspect under other exploratory conditions. After careful
critical analysis it has been interpreted as evidence of
coelution even when there should be one drug in the
sample. It was confirmed by the analysis of peak purity.
By comparing the chromatograms, it was concluded
that the sample loratadine, also contained
desloratadine, due to contamination or degradation. It
was possible to find a satisfactory condition. It was
found a condition capable of identifying and separating
loratadine and desloratadine: column temperature 25
°C, volume injection 20ul, flow 1.2 ml / min., DAD
detection with extraction at 247 nm, Varian column (50
x 4.6 mm, 5 um), mobile phase A-water / B-acetonitrile
as gradient (55% to 100% B - 0 to 8 minutes, 100% to
55% B - 8 to 9 minutes). Conclusion: Once one has
succeeded in the separation and purity peak, it is
suggested that this condition should be optimized under
chemometric techniques and validated to advance to
the biopharmaceutical studies.
Acknowledgment: UFOP/CiPharma/Ministério da
Saúde/Anvisa/FAPEMIG/Rede Toxifar/CNPq.
22
DISSOLUTION STUDIES OF ORAL SUSPENSIONS
AND BIOPHARMACEUTICAL CLASSIFICATION
USING NYSTATIN AS MODEL.
Rosa, L.S.1; Marques-Marinho, F.D
1; Souza, J
1;
1Universidade Federal de Ouro Preto, Escola de
Farmácia. Ouro Preto, Minas Gerais, Brasil.
INTRODUCTION: Dissolution studies are needed to
prove quality of manufactured medicines. Such
procedures are well established for solid oral dosage
forms, however, there is a lack of data for oral
suspensions. To evaluate the interferences in
dissolution studies of oral suspensions, the model will
be nystatin, a polyene antibiotic effective against
candidiasis in oral mucosa, skin and gastrointestinal
tract. This drug is commercially available as oral
suspension, which innovator medicine is Canditrat®.
OBJECTIVES: Considering the divergences related to
the biopharmaceutical classification of nystatin and
absence of established experimental conditions for
dissolution profiles of oral suspension, this work aims to
evaluate and establish conditions to execute dissolution
tests, perform solubility studies and summarize data
about permeability to then classify this drug in the
biopharmaceutical system. METHODS: A bibliographic
review will be made about the theme, development and
validation of a quantification method for nystatin in the
biorelevant media simulating gastrointestinal liquid used
in dissolution and solubility tests. A dissolution test for
comparison of profiles of commercialized nystatin will
be executed; the equilibrium solubility using the active
principle ingredient will be determined, permeability in
silico will be performed following data obtained from
scientific literature and databases. RESULTS: Early
tests showed that the viscosity of the oral suspension
when using a rotative viscometer in 100 rpm and L2
apparatus was 247 mPa.s, which indicates a low-
medium viscosity comparing with water, a low viscosity
liquid (0.890 mPa.S) or glycerin, a high viscosity liquid
(934 mPa.s); a scan in UV spectrophotometer (range
from 200 to 400 nm) showed that the maximum
wavelength absorbed was near to those preconized in
the Brazilian Pharmacopeia 5th edition: 291, 305 e 319
nm. However, the ratio between the absorbances were
out of the limit established in the Brazilian
Pharmacopea 5th ed , since the 296/318 nm (0.61 a
0.73), was 0.59, and the ratio 304/318 nm (0.83 a 0.96),
was 1.12. Permeability data obtained through free
access programs Marvin® and Mollinspiration®, and
were logP -1.04 and logP 0.21, respectively. These
results indicate low permeability for nystatin against
metoprolol, a high permeability control, whose logP
1.72.
ACKNOWLEDGMENTS: UFOP/CiPharma/ Ministério
da Saúde/Anvisa/Rede Toxifar. FUNDING:
FAPEMIG/CNPq/CAPES.
23
HEALTH CARE AND BIOPHARMACEUTICAL
ASPECTS OF DRUGS TO TREAT HIGH BLOOD
PRESSURE, DIABETES AND COMORBIDITIES:
SIMVASTATIN IN THE BIOWAIVER CONTEXT
ARAÚJO, L.U1,2
, FERREIRA, A.C.S2, SOUZA, J
2,
SILVA-BARCELLOS, N.M2
1Universidade Federal dos Vales do Jequitinhonha e
Mucuri – UFVJM 2Universidade Federal de Ouro Preto – UFOP
The health care with chronic non-communicable
diseases (NCDs) generates high costs to patients and
the Unified Health System. Thus, the study of NCDs,
the health care aspects in two towns and
biopharmaceutical aspects of applicable drugs
21
comorbidities of these diseases consist the general aim
of this work. Data from simvastatin study in the
biowaiver context will be display here. The
biopharmaceutical parameters such as solubility and
permeability, as well as drugs dissolution from the
dosage form are used to classify the drug into four
different classes according to the Biopharmaceutical
Classification System (BCS). In this context BCS has
been used as a regulatory tool aiming at the
replacement of in vivo bioequivalence studies by in vitro
test, what we call biowaiver. This study review and
discuss in detail existing information in the scientific
literature related to biopharmaceutical aspects of
solubility, permeability and dissolution of the
simvastatin and compile sufficient information to
support a safe discussion about the possibility of
biowaiver. Databases such as Capes, Medline,
ScienceDirect, Scopus and Web of Science were the
sources used for research. Simvastatin’s solubility and
permeability, its therapeutic use and therapeutic index,
pharmacokinetic properties, data related to the
possibility of excipient interactions, and reported
bioequivalence, bioavailability and dissolution data
were taken into consideration. The available data
suggest that according to the BCS and all current
guidances, simvastatin would be assigned to BCS class
II (low solubility and high permeability), with rapid
dissolution from dosage forms, action and metabolism
in liver. Also, in view of its therapeutic use, its wide
therapeutic index and action and metabolism in liver, a
biowaiver for immediate release simvastatin solid oral
drug products could be justified according to the World
Health Organization, if strictly follow the requirements
for immediate release products containing drugs of this
class: product contains only those excipients reported
in this paper in their usual amounts, the dosage form is
rapidly dissolving (85% in 30 minutes or less) in buffer
pH 6.8 and the test product also exhibits similar
dissolution profiles to the reference product in buffer pH
1.2, 4.5, and 6.8.
Acknowledgements: UFOP, FAPEMIG and CAPES.
24
SYNTHESIS OF POLY(LACTIC ACID) (PLA) WITH
ALLYL PENDANT GROUP AND DEVELOPMENT OF
NANOSPHERES
Luan Silvestro Bianchini Silva1, Gwenaelle Pound-
Lana1, Vanessa Carla Furtado Mosqueira
1,
1Laboratório de Desenvolvimento Galênico e
Nanotecnologia (LDGNANO), CiPharma, UFOP.
A major challenge addressed in the pharmaceutical
field of nanotechnology is the development of polymeric
nanocarriers with functional ligands on the surface,
allowing such systems to bind to specific cell sites. PLA
is a histocompatible polymer, biodegradable and which
degradation products are non-toxic; copolymerization of
lactide with allyl glycidyl ether (AGE) provides a
versatile platform for the insertion of ligands along the
polymer chain. In this context, the synthesis of PLA-co-
AGE polymer is interesting. The aim of this study was
to synthesize the copolymer PLA-co-AGE and develop
nanospheres with a chemically modifiable group. The
copolymer PLA-co-AGE was synthesized by ring
opening polymerization (ROP) from comonomers D,L-
lactide and AGE; Sn(Oct)2 as a catalyst and benzyl
alcohol as a co-initiator. The parameters Mn, Mw and
dispersity (Đ) of the copolymers were determined by gel
permeation chromatography (GPC). Nanospheres were
prepared via the nanoprecipitation method using the
synthesized copolymer and were characterized in terms
of size and Đ via the technique of photon correlation
spectroscopy (PCS) and of their zeta potential via the
technique of microelectrophoresis coupled to
anemometry laser Doppler (LDA). The characterization
by GPC showed a polymer with Mn = 25,600 g/mol and
Đ = 1.28; which characterizes the copolymer as uniform
and within a suitable range for nanocarrier preparation.
Stable Nanospheres were obtained with this copolymer,
having a reduced size (86.21 nm ± 1.05) and low Đ
(0.12 ± 0.02), the zeta potential was (-13.43 mV ±
0.64). The negative value obtained is expected for PLA
nanocarriers. PLA-co-AGE copolymer was synthesized
and stable nanospheres were obtained. The insertion of
a chain coupling agent, such as AGE, in the chains of
PLA is a strategy that can allow the coupling of
biological ligands along the polymer chain and therefore
the preparation of vectorized nanocarriers.
Acknowledgement: LDGNANO, Laboratório
Multiusuário do Cipharma. Financial support: CAPES.
25
CLEANING METHODS AND AIR QUALITY
CONTROL IN THE PROCESS OF COSMETIC
PRODUCTS MANUFACTURING AND PERSONAL
HYGIENE
Luana Beatriz Araujo Vaz1, Vanja Maria Veloso
2, Flávia
Dias Marques Marinho2, Jacqueline Souza
2, Iara
Gizelle Carvalho Silva3, José Mauro Nahim Trópia
2 e
Maria do Pilar Pinto Coelho Santos2
1Escola de Farmácia, UFOP, Ouro Preto, Minas Gerais,
Brasil. 2Fábrica de Cosméticos de Ouro Preto – Pérola.
Introduction: The factory Pérola Ouro Preto was
created by Fundação Sorria to generate income
through the production and marketing of cosmetics and
personal hygiene products in order to ensure their
22
sustainability and the foundation. In the quest for
competitiveness and meet the quality requirements of
the consumer market, it is necessary implementation of
cleaning and sanitization procedures, as they are
factors that can interfere with the physical, chemical
and microbiological properties of the products. In this
context, it is important to note that the indoor air quality
of the manufacturing environment can affect the quality
of the final product. Thus, it is important to emphasize
the need for air monitoring and the establishment of
parameters for analysis, to contribute to improving the
quality of products. Little has been documented in the
literature on these parameters, bering necessary to
establish methods and acceptance criteria for
evaluation. Objective: Evaluate cleaning methods used
in the manufacturing of cosmetics and toiletries
process, and analyze the influence of air quality control
in the production ambient. Methodology: If wish to
monitor the quality control of internal air through
sedimentation plates to evaluate the microbial load, and
also to evaluate the control unit and room temperature.
Validation of cleaning will be tested two methods of
sanitizing the equipment and then evaluating the
efficacy and fungal microbial removal, besides the
reduction of cross-contamination. Expected results: It
is hoped that this work establishing cleaning methods
and air quality control in production ambient of the
cosmetics and toiletries. The aim is to optimize the
cleaning process for the production of cosmetics
partner factory, minimizing reprocesses, cost savings,
reduced waste generation, can serve as a model for
other companies.
Supports: This project has the support of the
Universidade Federal de Ouro Preto, of the Escola de
Farmácia and of the Coordenação de Aperfeiçoamento
de Pessoal de Nível Superior.
26
NOVEL SYNTHETIC CHALCONES INDUCES
APOPTOSIS IN HUMAN GLIOBLASTOMA CELLS
Lucas Felipe Fernandes Bittencourt1, Karen Andrinéia
de Oliveira1,2
, Carine Bropp Cardoso1, Flávia Garcia
Lopes1, Tharine Dal-Cim
2, Louise Domeneghini
Chiaradia-Delatorre3, Alessandra Mascarello
3, Rosendo
Augusto Yunes3, Ricardo Castilho Garcez
1, Carla Inês
Tasca2 and Cláudia Beatriz Nedel
1*
1Laboratório de Biologia de Gliomas, Centro de
Ciências Biológicas, Universidade Federal de Santa
Catarina, Campus Trindade, CEP: 88040-900,
Florianópolis-SC, Brasil. 2Laboratório de Neuroquímica-4, Centro de Ciências
Biológicas, Universidade Federal de Santa Catarina,
Campus Trindade, CEP: 88040-900, Florianópolis-SC,
Brasil. 3Laboratório Estrutura e Atividade, Departamento de
Química, Universidade Federal de Santa Catarina,
Campus Trindade, CEP: 88040-900, Florianópolis-SC,
Brasil.
The glioblastoma multiforme is the main tumor of the
central nervous system and the most frequent in adults.
With a survival average of 5% two years after
diagnosis, this type of cancer is a main health issue.
Substances like the chalcones have been tested in
order to develop new treatments. In this work, we
studied the effects of three novel synthetic chalcones
(A23, C31 and J11) on A172 commercial cell lineage
and in surgery obtained-glioma cells, hereby referred as
GBM1. All chalcones showed a decrease in cell viability
by MTT cell viability assay, in both cell lines with 24 and
48 hours, especially C31. No effects on normal cells (rat
astrocytes) were observed. Also, C31 was the only
chalcone that caused an increase in apoptosis levels
with no further increase of necrosis; detected by
Annexin V assay kit in flow cytometry. Using the DCF-
DA and DAF-2 fluorimetric detection kits we suggest
that this augmentation may be linked to the high
oxidative effect found, caused by increased presence of
reactive oxygen species and nitric oxide levels. Flow
cytometry using propidium iodide revealed that C31
affected the cell cycle distribution, causing an arrest at
G0/G1 and S phases, suggesting that C31 interfere in
the cell cycle control, preventing neoplastic cells to
complete the mitosis cycle. Our results show that C31 is
a putative safe new antineoplasic substance that
induces apoptosis in a ROS-dependent manner,
causing cycle disruption and an increase in NO levels.
This study shall aid and direct future research regarding
this and similar substances and their antiproliferative
effects.
Acknowledgements: We wish to thank the Brazilian
funding agencies CAPES/CNPq for the financial
support.
27
PET PHARMACY UFOP RETROSPECTIVE AND
IMPORTANCE IN THE PROFESSIONAL AND
SCIENTIFIC PREPARATION
Amariz, A. P1; Silva, D. S
1; Alves, J.V.S
1 ;Rosa, J. S
1;
Silva, J.E.S1 ; Paixão, L. G
1; Oliveira, M. A
1; Matos, M.
B1; Cordeiro, N. N
1; Watai, P
1; Mendes, P. F
1;
Conceição, R.R1; Lima, R. R
1; Silva, S. S. C
1; Santos
T.M1; Ostolin, T. L. V. D. P
1; Souza, J
1;
1Programa de Educação Tutorial - Escola de Farmácia
-Universidade Federal de Ouro Preto.
23
Tutorial education program starts in 1979, in the
Coordenação de Aperfeicoamento de Pessoal de Nível
Superior-CAPES, being transferred in 1999 to the
Ministry of Education. PET is an academic investment
on the graduation courses that has as goal preparing
recognizably qualified professionals for creating, with
potential, activities that demonstrate a unique
preparation, qualified and recognized by the society.
PET Pharmacy started in april of 1992, and during
these 23 years of existence, the program counted with
6 tutors and about 70 students. The PET Pharmacy
group of Ouro Preto University is currently composed
by 16 students from all stages of the Pharmacy
graduation course and coordinated by a tutor professor,
Dr. Jacqueline de Souza. The main goal of the group is
the encouragement to collective learning, through
experiences, reflections and discussions in an
environment of cooperation, approaching to activities
oriented by the principle of non-dissociation between
teaching, research and continuing education and
tutorial education. Among the activities developed, the
Congress of Pharmaceutic Sciences of Ouro Preto,
which will have its fifth edition in 2016, is remarkable,
symposiums, improvement courses and continuing
education projects and research aiming the community
of Ouro Preto. All the activities have as goal
complement the academic preparation of the students
and meet the needs of the graduation degree. A survey
has been taken on the professional status of the
students who participated in the program, seven of
those are currently post-graduation students in UFOP
and five of them are part of CiPharma. Post-graduated
in Pharmacy or are post-graduation students in other
institutions, not mentioning those who followed a
professional career and excel in the work field. The
professional improvement provided by PET is
unanimous, being remarked specially: leadership,
speech, group work, learning how to organize events,
interaction with the community, mainly from continuing
education projects. Since it is also necessary run
research projects in PET, it is believed that it might
have encouraged many students to follow the research
field, considering that many of them did a strictu sensu
post-graduation or are currently doing it. According to
the collected data, one can affirm that PET-Pharmacy
contributed, not only during graduation, but also in the
future of those who made part of it, providing learning
and abilities that are essential in professional life.
Thanks to UFOP and Ministry of Education.
28
Effects of silibinin on AKT2 gene and miR-203
expression in bladder tumor cells
Ludmila Correia Barbosa1, Glenda Nicioli da Silva
1.
1UFOP – Universidade Federal de Ouro Preto, Escola
de Farmácia, Departamento de Análises Clínicas, Ouro
Preto, MG, Brazil.
Cancer is the second cause of death in Brazil and in the
world, leading to 8.2 million deaths, 14.9 million
incident cases and 196.3 million disability-adjusted life-
years in 2013. Among these tumors, the urothelial cell
carcinoma (UCC) is the most common genitourinary
malignancy, presenting a high prevalence, especially in
industrialized countries.Disturbances in pathways of
PI3K/AKT2 are associated with a majority of sporadic
human cancers, as breast carcinoma, ovarian and
bladder cancer. Treatment protocols for UCC include
the MVAC, a combination of methotrexate, vinblastine,
doxurrubicin and cisplatin; and the association of
gemcitabine and cisplatin, but they presents evere side
effects such as high toxicity. In this sense,the searching
for alternative treatments, especially the identification of
potential anticancer compounds isolated from natural
products, such as silibinin(wild artichoke),has deserve
special attention. Phytochemical products derived from
medicinal plants have some advantages such as better
compatibility with the human body, few side effects, and
are less costly and relatively non-toxic. However, the
molecular mechanisms of silibinin involved in the
observed changes on cell cycle kinectics and apoptosis
rates in tumoral cells were not fully elucidated,
especially when cells with different status of TP53 gene
(a main genetic alteration observed in bladder tumors)
were studied. Therefore, the aim of this study was to
characterize molecular events such as AKT2 gene and
microRNAs expression changes in bladder tumor cells
with wild (RT4) and mutated (T24) TP53 gene treated
with silibinin(135 µM, 150µM and 200 µM).The results
showed that silibinin decreased AKT gene expression
only in RT4 cells after different treatments with silibinin.
However, miR-203 expression modulation was not
observed in both tested cell lines. These data show
action mechanism of silibinin might depend on the
TP53 status.
Financial support: FAPEMIG, CNPq and UFOP
29
Evaluation of Type I Laboratory (Pharmacy School-
UFOP) cervical cytological diagnostics of submitted
to external quality control
Mariana Trevisan Rezende1; Alessandra Hermógenes
Gomes Tobias1; Claudia Martins Carneiro
1,2.
1CIPHARMA/Pharmacy School/Federal University of
Ouro Preto/Ouro Preto/MG. 2Cytology Sector/Pilot Laboratory of Clinical
Analysis/Pharmacy School/Federal University of Ouro
Preto/Ouro Preto/MG.
24
The external quality control has been proposed as a
method able to improve performance of laboratories
that performs cytological exams for a public health
service, through minimizing interobserver variation,
which is a major cause of the high rates of false-
negative results. The objective is to evaluate the
diagnoses of cytological exams issued by the Type I
Laboratory of Pharmacy School of UFOP comparing
with the results released by Type II Laboratory
responsible for conducting external quality control in
Minas Gerais state. The frequency of concordant and
discordant cases, of false-negatives and false-positives
by Type I Laboratory will be calculated according the
agreement with the Type II Laboratory results.
Moreover it will be calculated the Type I Laboratory
quality indicators before and after evaluation by Type II
Laboratory. The frequency of discordant cases that had
change clinical management will be determined. The
methods of internal quality control will be analyzed
against the results Type II Laboratory.The quality of
slidescoloring and assembling of Type I Laboratory will
be examined in accordance with the required quality
criteria by Qualicito/2013. Thus, we expect to identify
the major causes of errors and discrepancies
diagnostics. With training and continuing education,
professionals involved in Pap smear testing can
standardize criteria cytomorphological, improving
performance in the diagnosis of precursor lesions of
cervical cancer.
Acknowledgements: CAPES, UFOP.
30
EVALUATION OF THREE PRIMERS FOR HPV
DETECTION BY CONVENTIONAL PCR IN FFPE
CERVICAL SAMPLES
Silva, NNT1, Tafuri, A
2, Lima, AA
1
1Escola de Farmácia, Universidade Federal de Ouro
Preto;
2Laboratório Tafuri;
Introduction: The detection of human papillomavirus
(HPV) is an important tool to complement the diagnosis,
treatment and cervical cancer screening. The main form
HPV detection is through the viral DNA amplification by
polymerase chain reaction (PCR). However, is difficult
to perform this technique in formalin fixed paraffin
embedded (FFPE) samples due to the DNA
degradation that occurs during the fixing process and
paraffinization. Objective: To compare the HPV
detection results in cervical biopsy samples using PCR
with three different primers: MY09/MY11 [PCR
(MY09/MY11)], GP5+/GP6+ [PCR (GP5+/GP6+)] and
SPF [PCR (SPF)], to choose the best methodology for
HPV detection in FFPE samples. Material and
Methods: 150 cervical biopsy blocks were selected ang
divided into 5 groups of 30 samples [Cervicitis (control),
cervical intraepithelial neoplasia (CIN) grades I, II and
III, and invasive cervical carcinoma (ICC)]. DNA
extraction was performed with DNA FFPE Tissue
QIAamp® Kit (QIAGEN). The HPV detection was
performed by PCR (MY09/MY11), PCR (GP5+/GP6+)
and PCR (SPF) that generate fragments of 450bp,
160pb and 95pb, respectively. Results: HPV was not
detected in any sample using PCR (MY09/ MY11), so
this technique was excluded in other analyzes. The
concordance index (Kappa) between PCR
(GP5+/GP6+) and PCR (SPF) was very light (κ=0.022).
The HPV positivity analyzed by PCR (SPF) was high
(96.7%, n=145) and higher than that found by PCR
(GP5+/GP6+) (24.7%, n = 37). Higher positive
correlation was noted between the results obtained with
PCR (SPF) and PCR (GP5+/GP6+) in samples
presenting lesions compared to the control group.
Considering the different types of cellular alterations,
increased positive correlation between the two methods
was observed with the evolution of the lesion degree.
Among the samples that the infection was detected by
both methods, the most showed ICC (43.3%, n=13).
According to the age, most positive agreement has
been found in samples of women aged >30 years old.
PCR (SPF) showed higher sensitivity compared to PCR
(GP5+/GP6+). Conclusion: This study showed that the
PCR using the SPF primer set showed better results for
HPV detection in FFPE samples.
Financial Support: Conselho Nacional de
Desenvolvimento Científico e Tecnológico (CNPq) e
Coordenação de Aperfeiçoamento de Pessoal de Nível
Superior (CAPES).
31
CARDIOPROTECTIVE ACTIVITY OF IPRIFLAVONE
IN SPONTANEOUSLY HYPERTENSIVE RATS
SUBMITTED TO THE LEFT CORONARY LIGATURE
CASTRO Q.J.T.1, MOSQUEIRA V.C.F.
1, PEREIRA
S.C.1, SOUZA A.C.M.
1, AMANCIO, G.C.S.
1,
GUIMARÃES H.N.2, LEITE R.
1, GRABE-GUIMARÃES
A.1.
1CiPharma, Escola de Farmácia, UFOP, Ouro Preto,
MG; 2DEE, Escola de Engenharia, UFMG, Belo Horizonte,
MG, Brazil.
Introduction: Ipriflavone (7-isopropoxy-3-phenyl-4H-1-
benzopyran- 4-one) is a semi-synthetic soy derivative,
used in several countries for prevention and treatment
of osteoporosis. Its cardioprotective effect administered
orally was demonstrated in isolated heart of rabbits
25
(Feuer et al, 1981). Self-emulsifying drug delivery
systems (SEDDS) have gained great importance as a
promising technology to improve the bioavailability of
poorly water-soluble drugs like ipriflavone. Objective:
The objective was to evaluate the cardioprotective
effect of ipriflavone in self-emulsifying drug delivery
system (SEDDS) administered in female spontaneously
hypertensive rats (SHR) submitted to the left coronary
ligature. Methods: All the procedures were approved
by CEUA/UFOP (2013/02). The rats were submitted
first to the heart coronary ligature and treated during 21
days with ipriflavone-SEDDS (30 mg/kg) or with blank
SEDDS, in single daily dose. Sham animals treated
with ipriflavone-SEEDS or blank SEDDS were used as
control group. At the 22th day, under anesthesia
(ketamine 100 mg/kg/xylazine 14 mg/kg), the lead II
ECG signal was obtained. The cardioprotective activity
of ipriflavone was evaluated for its ability to prevent
cardiovascular disorders, demonstrated in signs of
ECG induced by IV administration of norepinephrine
(NE), doses of 3 and 10 µg/kg. Results: For the PR
and QRS intervals there were not significantly
differences in animals treated with vehicle or
ipriflavone. The QT and QTc increases after the ligature
were significantly reduced in ipriflavone treated animals
compared to the vehicle treated animals. The reduction
of the QT prolongation induced by 3 and 10 µg/kg of NE
respectively was 78% and 73%. And the reduction of
the QTc was 58 % and 53 %, after 3 and 10 µg/kg,
respectively. Conclusion: The QT and QTc intervals
prolongation are predictors of cardiac arrhythmias, thus
the reduced alterations induced by NE in SHR treated
female rats reveals the ipriflavone-SEDDS
cardioprotective activity and its potential for further
clinical investigation.
Funding agencies: FAPEMIG (Rede Nanobiomg) and
UFOP
Acknowledgments: CNPq; UFOP; CAPES and Project
APQ 02346-11 (FAPEMIG)
32
Morpho-anatomic and Phytochemistry Studies in
Leaves of Abatia americana(Gardner) Eichler
(Salicaceae)
Regislainy Gomes da SILVA1*
, Hildeberto Caldas de
SOUSA2, Gustavo Henrique Bianco de SOUZA
1
1Universidade Federal de Ouro Preto-MG,
Departamento de Farmácia - Escola de Farmácia;
Laboratório de Fitotecnologia, Campus Morro do
Cruzeiro, s/n CEP 35400-000 Ouro Preto – MG, Brasil.
2Universidade Federal de Ouro Preto-MG,
Departamento de Biodiversidade, Evolução e Meio
Ambiente - Instituto de Ciências Exatas e Biológicas;
Laboratório de Anatomia Vegetal, Campus Morro do
Cruzeiro, s/n CEP 35400-000 Ouro Preto - MG, Brasil.
*autora para correspondência: [email protected].
Biologically active metabolites emerged in nature by
ecological needs and biosynthetic possibilities.
Secondary metabolites often present interesting
biological activities, in spite of they have been
considered as plant waste products. Phenolic
compounds, a class of secondary metabolites are
formed in plants under stress, and flavonoids, a
subclass of phenolic compounds, have various
pharmacological functions described in the literature,
such as antiviral, antioxidant and anti-inflammatory. The
Salicaceae family often has phenolic glycosides usually
with tannins, but, in general, they lack cyanogenic
glycosides. Casearia sylvestris Sw., a specie of this
family, has biological activities already mentioned by
the reasearches, such as antitumor, antiviral,
antibacterial, antifungal, molluscicide,
hypocollesterolemic and analgesyc. Salix alba L.,
another specie from this family was the source of
salicilic acid, molecule that origined the acetilsalicilic
acid, commercialized until today, for several
pharmacologial activities. Abatia americana (Gardner)
Eichler, from Salicaceae family, is a native specie,
endemic to Brazil, and doesn’t have a popular use
described yet; therefore it lacks research on its possible
pharmacological applications. In this project, a botanical
and phytochemicals study were conducted to determine
the morphological and anatomical characteristics of its
leaves. To this end, fully expanded leaves were
collected in Ouro Preto; part of this material was fixed in
FAA70, and after 48 hours stored in 70% ethanol.
Transversal cuts and epidermis dissociations were
made in order to perform morphoanatomic study. The
leaf lamina is dorsiventral and hypostomatic. There are
multiseriate adaxial epidermis and uniseriate abaxial
epidermis with paracytic stomata and tectores
ornamented trichomes on both sides. It has collateral
vascular bundle in an open arc. In order to carry out
phytochemical studies, another part of the collected
material was stored in plastic bags, dried and
pulverized, and then subjected to sequential extractions
by means of percolation technique, with the intention of
isolating major secondary metabolites. Identification
was made using chromatographic techniques such as
Thin Layer Chromatography Comparative and
Preparative, Classical Liquid Chromatography and Ultra
Performance Liquid Chromatography acopled to Mass
Spectrometry. In conclusion, the anatomical features
are important to characterize the future herbal medicine
and has been identified the flavonoid lucenin-2.
Key-words: Abatia americana (Gardner) Eichler,
lucenin, flavon.
26
Acknowledgements: This work was supported by
CAPES (Coordenadoria de Aperfeiçoamento Pessoal
de Nível Superior), by CIPHARMA (Programa de Pós-
Graduação em Ciências Farmacêuticas), by
Laboratório de Fitotecnologia and by Laboratório de
Anatomia Vegetal from Universidade Federal de Ouro
Preto, Minas Gerais, Brazil.
33
A KNOWLEDGE EXCHANGE BETWEEN UNIVERSITY AND COMMUNITY AS AN INSTRUMENT TO MITIGATE SOCIAL PROBLEMS
Rodrigo Chaves Ângela L. Andrade (PQ), Carolina
M.S.A. Maranhão (PQ), Diego L.N. Ventrella (PQ),
Fábio V. Moura (PQ), Kerley S. Alves (PQ), Tays T.R.
Chagas (PQ), Vera L.M. Guarda (PQ)
Professores do Núcleo da Cátedra UNESCO: Água,
Mulheres e Desenvolvimento da Universidade Federal
de Ouro Preto, Ouro Preto, Minas Gerais, Brasil
Introduction: The occupation of Antônio Pereira district
of Ouro Preto, dating from the eighteenth century. The
height of the growth of this district occurred in the 70s,
with the installation of large mining companies in the
region, including mining company Vale do Rio Doce
and Samarco. After this growth phase, Antonio Pereira
went into decline, which was only eased in the 1980s,
when the district received new mining companies that
still operate in the region. Currently, mining companies
are coming back into recession, causing large numbers
of unemployment. Adding to this scenario, we have a
population dominated by women, many single mothers
and unemployed. In an attempt to modify part of this
situation, UFOP teachers came together to look for
ways to improve the quality of life of a group of women,
through new ways of generating income from the
creation of associations. Objectives: Increase the self-
esteem of women participants; empower associates in
new role and business activities; legalize associations;
develop new knowledge production and management
of a Community collective. Methods: During the project
were offered training courses in business management,
marketing, sales and cooperatives, where, taking
advantage of the expertise of teachers in charge of
related fields, was seeking a sustainable productive
arrangement in the financial and operational level.
These associations were structured for the production
of cleaning products, crafts and making clothes and
uniforms. Results: The achievements include working
knowledge of production equipment in the associations,
hierarchy establishment through internal voting, working
knowledge of routines employed, gradual increase in
profit, improved self-esteem, and coexistence. From the
completion of the courses, and analyzes problems
occurred during the project, it may be noted that
member is being able to run the business without the
help of the University, despite the constant support.
Conclusion: After completion of training courses and
early operational management process of associations,
there was a personal growth, self-esteem and desire for
finding new forms of income of women generation, will
this perceived from the commitment of women to hold a
profitable venture for all.
Thanks and Financial Support: The project thanks
mainly to the Federal University of Ouro Preto,
FAPEMIG, Fundação Gorceix, NuCat and mining
company Samarco, at both financial and operationally
unrestricted support of the project.
34
EVALUATION OF ACCELERATED BLOOD
CLEARANCE PHENOMENON MICE INFECTED WITH
LEISHMANIA INFANTUM TREATED WITH
PEGYLATED LIPOSOMES FORMULATIONS
CONTAINING TARTAR EMETIC
Tamara Cristina Moreira Lopes¹; Débora Faria Silva¹;
Walyson Coelho Costa¹; Wanderson Geraldo de Lima2;
Adriel Araújo Fernandes Ferreira3; Neila Márcia Silva
Barcellos1; Rodrigo Dian de Oliveira Aguiar Soares
4;
José Mario Barichello¹; Simone Aparecida Rezende¹.
1CiPharma - Escola de Farmácia – Universidade
Federal de Ouro Preto (UFOP) 2Departamento de Ciências Biológicas – UFOP
3Departamento de Fisiologia e Biofísica – Universidade
Federal de Minas Gerais (UFMG) 4Núcleo de Pesquisa em Ciências Biológicas – UFOP
Accelerated blood clearance (ABC) phenomenon has
been observed during the repeated administration of
PEG-conjugated nanosystems, including PEGylated
liposomes (LPEG). ABC phenomenon promotes the
capture of LPEG macrophages during therapy. We
evaluated if ABC phenomenon modifies the toxicity and
targeting of LPEG of tartar emetic (TE) to liver and
spleen in a murine model of leishmaniasis. We also
evaluated if the ABC phenomenon improves the
efficacy of leishmaniasis therapy. The liposome
formulations were characterized by size, polydispersity
index and drug encapsulation. The hemolytic activity of
formulations and blood count were correlated. Non-
infected and Leishmania infantum infected mice were
divided in two groups, one in which ABC phenomenon
was not induced: 1) PBS; 2) free TE; 3) empty
conventional liposomes (LC); 4) LC+TE; 5) LPEG+TE;
and another in which ABC phenomenon was induced
27
by the endovenous administration of empty LPEG: 6)
LC+TE; 7) empty LPEG and 8) LPEG+TE. After two
weeks the animals were euthanized and biometric
parameters were evaluated. The level of antimony (Sb)
in the tissues was accessed in non-infected animals.
Histological analyses were performed to evaluate the
therapy toxicity. All formulations showed size of
200nm, polydispersity index 0.3 and drug
encapsulation efficiency of 14%. TE formulations and
free TE induced low in vitro hemolytic activity (15%).
Non-infected mice showed no blood count alteration
due to treatment. No alteration was observed in body,
liver or spleen mass. Treatment with free TE induced
higher level of Sb in liver. Among the liposomal
formulations, treatment with LPEG+TE with ABC
phenomenon was able to increase Sb level when
compared to LPEG + TE without ABC phenomenon. In
the spleen, higher levels of Sb were observed in mice
treated with LPEG+TE without ABC phenomenon in
relation to treatment with conventional liposomes of TE.
In relation to histopathological analysis, non-infected
animals showed no alteration in liver and spleen due to
treatment. Treatment with free TE and liposomal
formulations of TE was able to reduce parasite load in
liver. Otherwise, in spleen and bone marrow, only
PEGylated liposomes of TE were able to suppress
parasite load. No alteration in parasite load was
observed in ABC phenomenon groups. Although ABC
phenomenon favors the accumulation of TE in the liver,
it no changes the parasitism in the organs. Only LPEG
of TE reduced parasite load in the organs.
Financial support: CAPES, CNPQ and UFOP.
35
BIOPHARMACEUTICAL POTENTIAL OF A
BIOLOGICALLY ACTIVE SUBSTANCE ISOLATED
FROM TYPICAL BRAZILIAN CERRADO PLANT
Caldeira, T. G.1; Saúde-Guimarães, D. A.
1; Souza, J.
1
1Curso de Pós-Graduação em Ciências Farmacêuticas
- CiPharma - Universidade Federal de Ouro Preto,
Ouro Preto, Minas Gerais, Brasil.
Biopharmaceutical parameters consisting of solubility
and permeability characteristics and are directly related
to the degree of a drug or biologically active substance
absorption along the gastrointestinal tract. Therefore, in
1995 was proposed the Biopharmaceutics
Classification System (BCS), which classifies drugs into
four classes according to solubility and permeability
characteristics, and subsidizes the replacement of in
vivo studies, such as bioavailability and/or
bioequivalence, for in vitro assays, which allow
conclusions about the solubility in biological fluids and
permeability of drugs. Besides, this system is also used
in research the new molecules with potential
pharmacological. In this context, a biologically active
substance isolated from typical specie of the Brazilian
Cerrado has been studied due to promising
trypanocidal, antitumor, anti-inflammatory, anti-
hyperuricemic and anti-gouty arthritis activities and low
toxicity. Regarding the biopharmaceutical studies, this
substance has a low solubility in silico and in
equilibrium, exhibiting LogS between -3.83 and -4.33,
values comparable to other poorly soluble drugs on the
market today. And, high permeability, to present LogP
(2.00) comparable to drug metoprolol (LogP = 1.80) and
effective permeability (Peff = 34.40 x 10-6) higher than
the high permeability standard drug, propranolol
hydrochloride (Peff = 9.23 x 10-6). Considering the
initial biopharmaceutical characteristics of the
substance, pharmacological activity and low toxicity is
necessary to evaluate the variables that can affect the
process of absorption of this along the gastrointestinal
tract. Therefore, this study aims to evaluate the
biopharmaceutical potential for biologically active
substance isolated from typical specie of the Brazilian
Cerrado, by determining the intrinsic dissolution rate
and the permeability of Caco-2 cells. The intrinsic
dissolution rate will be standardized, validated and
determined in biorelevant media: acetate buffer (pH
4.5), intestinal fluid simulated without enzymes (pH 6.8)
and phosphate buffer (pH 7.4). Similarly, the
permeability technique evaluation using Caco-2 cells
undergo validation and standardization. Expected that
the experimental procedures established to clarify more
effectively the behavior this substance along the
gastrointestinal tract, since that polymorphism and
absorption by carriers will be evaluated. Thus
contributing for the development of a suitable
pharmaceutical formulation to promote maximum oral
bioavailability, coming this be a new therapeutic option
for the treatment of various diseases by the public
health service.
Acknowledgements: UFOP/CiPharma/Ministério da
Saúde/Anvisa/FAPEMIG/Rede Toxifar/CNPq/CAPES.
Key-words: Solubility, Permeability, Intrinsic
dissolution, Caco-2 cells, Biologically active substance.
36
EFFECTS OF THE SILIBININ (SILYBUM MARIANUM)
ON FRAP/MTOR GENE AND MICRORNA 100
EXPRESSION IN BLADDER TUMOR CELLS
Tatiane Martins Barros1, Glenda Nicioli da Silva
1
28
1Escola de Farmácia, Departamento de Análises
Clínicas, Universidade Federal de Ouro Preto- UFOP-
Ouro Preto, MG, Brazil.
Bladder cancer is characterized by being a highly
morbid disease due to high recurrence rates. Genetic
alterations in TP53 gene are related to cellular
transformation, malignancy and tumor progression.
Moreover, disturbances in pathways of PI3K-
FRAP/mTOR are associated with a majority of
sporadic human cancers, as breast carcinoma, ovarian
and bladder cancer. The drugs used to treat bladder
cancer present high toxicity and adverse side effects.
Thus, the searching for alternative compounds to
improve the efficacy of standard treatments and to
reduce the undesirable side effects has become
imperative. Among the compounds studied with
potential for anticancer activity in the bladder, the
silibinin, a naturally occurring flavonoid isolated from
the fruits and seeds of milk thistle (Silybum marianum),
is one of the less evaluated. The aim of this study was
to evaluate the effects of silibinin (135 µM, 150 µM and
200 µM) on miR-100 and its target FRAP/mTOR gene
expression in tumor cells of the bladder with wild (RT4)
and mutated (T24) TP53 gene. The results showed that
silibinin decreased FRAP/mTOR gene expression only
in RT4 cells after different treatments with silibinin.
However, miR-100 expression modulation was not
observed in both tested cell lines. In conclusion,
changes in mTOR mechanism may be associated with
antiproliferative effects of silibinin in the wild TP53 cells.
Financial support: FAPEMIG, CNPq and UFOP.
37
COMPOUNDS IDENTIFIED ON THE LEAVES OF
PROTIUM SPRUCEANUM (BENTH.) ENGLER BY
GC-MS
Tatiane Roquete Amparo1, Adriana Akemi Okuma
2,
Sidney Augusto Vieira Filho1, Vivette Appolinário
Rodrigues Cabral3, Gustavo Henrique Bianco de
Souza1
1Universidade Federal de Ouro Preto, Departamento de
farmácia;
2Centro Federal de Educação Tecnológica de Minas
Gerais, Departamento de química;
3Universidade Federal de Lavras, Departamento de
Ciências Florestais
Due the chemical diversity of the medicinal plants the
phytochemical studies are important to the discovery of
new drugs. Protium spruceanum (Benth.) Engler,
known as almécega-de casca-lisa or breu, is used by
the population like anti-inflammatory and expectorant,
however, it is necessary to expand studies about this
specie. The aim of this work was the identification of the
volatiles components of leaves of P. spruceanum. The
crude extract (37,54 g) was obtained by maceration of
the fragmented leaves (221,66 g) with ethanol (yield:
16,93 %). The extract (20,0 g) was resuspended in
Methanol:Water (1:1) and subjected to liquid-liquid
partition with hexane and ethyl acetate to obtain the
fractions: hexane (3,6 g), ethyl acetate (6,0 g) and
hydromethanolic (9,3 g). The extract and the fractions
were analysed by gas chromatography-mass
spectrometry (GC-MS). Sixteen compounds were
identified in the crude ethanolic extract and in the
hexane fraction. The majority in the crude extract was
methyl hexadecanoate and α- and β-amirin were the
majority in the hexane fraction. Four compounds were
identified in the ethyl acetate fraction and in the
hydromethanolic fraction, with the majorities:
hexanedioic acid, bis(2-ethylhexyl) ester and methyl
benzoate, respectively. These compounds were found
in others medicinal plants and can be associated to the
biological activities of P. spruceanum.
Apoio financeiro: FAPEMIG, CNPQ
38
INFLUENCE OF HYDROCHLOROTHIAZIDE RAW
MATERIALS IN SUSPENSION CHARACTERISTICS
SOARES, T.S.P.1; SOUZA, J.
1; MARQUES-MARINHO,
F.D.1;
1Laboratório de Controle de Qualidade, Departamento
de Farmácia, Escola de Farmácia, Universidade
Federal de Ouro Preto.
The thiazide diuretic hydrochlorothiazide (HCTZ) is a
water insoluble drug, only commercially available in
solid oral dosage form (SODF). Due to its low solubility,
HCTZ liquid oral dosage forms need to be prepared as
suspension. Suspension is a preparation of finely
divided drug in a suitable aqueous fluid vehicle. Drug
particles in the vehicle tend to sediment and the formed
sediment must be easily redispersed by agitation
(flocculated system), otherwise compact sediment is
formed (deflocculated system). After oral suspension
administration, the drug is available to immediate
dissolution in the biological fluids that represents
advantage to the SODF administration. Aiming to
propose dissolution test for HCTZ suspension, in this
part of the work, HCTZ raw materials (n=4) and their
respective suspensions had the physicochemical
properties evaluated. The suspensions´ storage at
different temperatures was also studied. HTCZ raw
29
materials were analyzed according to Brazilian
Pharmacopoeia tests: physical properties, solubility,
melting point, infrared and ultraviolet identification.
Suspensions of 2.5mg/ml and 5mg/ml were
compounded from each HCTZ sample and the batches
(1,2,3,4) were characterized regarding viscosity, pH,
sedimentation volume, redispersibility, zeta potential
and particles size. The freshly prepared suspensions
were evaluated and 15, 30 and 120 days after storage
at room temperature and at 8ºC. The HCTZ samples
met the pharmacopeia tests except the ultraviolet
identification. The viscosity of the suspensions at
2.5mg/ml and 5 mg/ml ranged from 150-125 mPa.s and
140-118 mPa.s, respectively. After 120 days, the pH of
all suspensions increased (max. 3.86) compared to the
initial pH (3.3). Sedimentation volume of freshly
suspensions (0.93-0.42) was greater than those after
120 days (0.70-0.39). Only batch 4 had a compact
sediment formation. Time required to sediment re-
dispersion ranged from 17 to 28s, with greater time
being observed for batch 4 (21-28s). After 120 days,
there was a reduction in the absolute zeta potential of
all the batches, except batch 4 that presented little
change. The suspensions showed different particle
sizes according to the batch: intermediate, batches 1-2;
bigger, batch 3 and much smaller, batch 4,
corroborating with the results of sedimentation volume
and redispersibility. The batches showed differences in
tests related to the particles characteristics as
redispersibility and zeta potential. Similar
characteristics were observed between suspensions
stored at room temperature and at 8ºC, so there is no
need to store in the refrigerator. Except for batch 4, all
other were classified as flocculated systems. Among
the batches, batch 2 was considered the most suitable
for the dissolution test.
Key-words: Hydrochlorothiazide. Dissolution. Raw
material. Suspension.
Acknowledgemets:CIPHARMA /EF /UFOP /FAPEMIG
/Anvisa/CAPES.
39
THERAPEUTIC POTENTIAL OF IPRIFLAVONE
CONVEYED IN A SELF-EMULSIFYING DRUG
DELIVERY SYSTEM (SEDDS) IN SEXUAL
DYSFUNCTION IN FEMALE SHR RATS DURING
THE CLIMACTERIC PERIOD
MARTINS T. A.1, MENDES J.C
1,RODOVALHO G. V.
1,
LEITE R.1
1CiPharma, Escola de Farmácia, UFOP, Ouro Preto,
MG;
Female sexual dysfunction (FSD) is a condition in
which there are alterations in the processes involved
in the female sexual response cycle. A decline in
serum estrogen, which occurs in menopause,
results in a significant decrease in the clitoral
intracavernosal, vaginal, and urethral blood flow,
thinning of vaginal mucosal epithelium and atrophy
of vaginal wall smooth muscle. The conventional
hormone therapy has proven effective in the
treatment of FSD, however, this therapy is associated
with an increased risk of the incidence of ischemic
cardiovascular events, as well as venous embolism.
Therefore, there is great interest in the development
of therapeutic alternatives that minimize the
deleterious effects of hypoestrogenism on sexual
function without causing side effects and avoiding
contraindications. The fitoestrogen ipriflavone
(7‐isopropoxy‐3‐phenyl‐chromen‐4‐one), a derivative
of daidzein (a naturally occurring isoflavone), is used
in the prevention and treatment of osteoporosis
however, the potential role of ipriflavone in the
treatment of FSD is unknown. The aim of this study
was to investigate the therapeutic potential of
ipriflavone in the treatment of FSD. For that, we used
young (4-5 months) and senescent (18-19 months)
female spontaneously hypertensive rats (SHR)
treated for 30 days with ipriflavona 10 or 30mg/Kg
conveyed in a self-emulsifying drug delivery systems
(SEDDS). Control group received only SEDDS
vehicle. Physiological parameters were evaluated
during pelvic nerve stimulation. Intravaginal
temperature significantly increased at the dose of 10
mg/Kg in the senescent SHR group. The vaginal
lubrication was improved after treatment with
ipriflavone 30 mg/Kg in the young and in the
senescent rats. Intravaginal pressure was
significantly higher in both young and senescent
groups treated with either 10 or 30 mg/Kg of
ipriflavone. Chronic treatment with ipriflavone with
doses of 10 and 30 mg/kg improved the
vasocongestive responses observed during the
subsequent 30 minutes interval after subcutaneous
injection of apomorphine (80μg/kg) in conscious young
and senescent female SHR rats. Furthermore, using
the same model to study sexual function in conscious
rats, the improvement in the vasocongestive reponses
observed in ipriflavone treated rats was similar to that
observed for the estradiol treated group. Histological
evaluation of liver and kidney, along with the
characterization of the lipid profile and some enzyme
levels (kidney and liver enzymes). Also, the amount of
intact glomeruli was significantly greater than the
degraded glomeruli, suggesting absence of hepatic
and renal toxicity. Therefore, the chronic treatment for
30 days with the ipriflavone phytoestrogen conveyed
in SEDDS, seemed to be safe and significantly
30
improved female rat sexual function, suggesting a
therapeutic potential for the treatment of the sexual
dysfunction induced by hypoestrogenism observed in
menopause/climacteric woman. Statistical analyses:
p<0.05 for all groups except for glomeruli quantitation
(p <0.001); ANOVA followed by Bonferroni post-
test.
Financial support: FAPEMIG, CAPES and UFOP.
40
EVALUATION OF METABOLIC SYNDROME IN
CLIMACTERIC WOMEN
Gouvea TM*1, Lopes ACF
1, Veloso VM
1, Carrillo MRG
1,
Lima AA1
1Escola de Farmácia/UFOP, Ouro Preto – MG
INTRODUCTION: The menopause corresponds to a
physiological transition in women's lives, where
changes are observed in physical, psychological and
social level. It has been associated with menopause, an
increased risk of obesity and metabolic syndrome, a
relationship which, although controversial, could be
explained by the decrease in basal metabolic rate,
physical inactivity and hormonal changes resulting from
this stage. The importance of studies and interventions
aimed at seeking healthy aging then it should be
recognized, as well as prevention and treatment of
cardiovascular risk factors.
OBJECTIVE: To determine the prevalence of metabolic
syndrome (MS) and its individual components in
menopausal women.
METHODS: Cross-sectional study with 75 women aged
45 to 60, selected in Basic Health Units
of Ouro Preto, Minas Gerais. The presence of MS was
determined according to the classification criteria of the
National Cholesterol Education Program's (NCEP-
ATPIII) and the International Diabetes Federation (IDF).
For this, glucose, total cholesterol and fractions, as well
as measuring blood pressure and waist circumference
were taken in the sample. Structured interviews with
participants enabled the raising of socio-demographic
and behavioral factors.
RESULTS AND CONCLUSION: The prevalence of MS
by the NCEP-ATPIII criteria was 48,0%, while at the
IDF, was slightly higher (53,3%). The agreement
between these two classifications was excellent (k =
0,894). There was no statistically significant association
between age, education, marital status, smoking and
alcohol with the development of MS. When the
components of MS were analyzed separately, the most
obvious change was abdominal obesity (waist
circumference: 96,1±14,2cm). The profile of the
selected women showed that the mean/standard
deviation age of the study population was 52,5±3,9
years; fasting glucose 112,3±47,4 mg/dL; HDLc
56,6±13,3 mg/dL; triglycerides 127,9±70,2 mg/dL and
mean arterial pressure 128x81 mmHg. Regardless of
classification method, this study showed a considerable
prevalence of menopausal women diagnosed with MS,
and waist circumference as amended factor.
ACKNOWLEDGMENTS AND FINANCIAL SUPPORT:
PROPP/UFOP, CAPES e FAPEMIG.
41
TRAINING FOR FOOD HANDLERS AND
CHAMBERMAID
Amanda Maria Silva Gonçalves; Fagner Parreiras Egg;
Thais Santos Rodrigues; Crislaine Custódia Rosa;
Kerley dos Santos Alves; Vera Lúcia de Miranda
Guarda.
Nucleus of the UNESCO Chair: water, women and
development.
Introduction: From 2011-14, 102 people, among which
82.35% women were trained in food handling by the
UNESCO Chair. In the same period, at the request of
VISA Mariana-MG we carried out a survey of the
bacteriological quality of snacks sold by bakeries, snack
bar and private salting. Of the 23 samples analyzed,
82.61% presented some type of contamination and
13% were rejected because they presented
contamination by Staphylococcus aureus and
Escherichia coli. Given this reality, the training in Food
handler showed up necessary because the study there
was noncompliance with the rules of Good Practices of
Food Handling.
The hosting industry, directly linked to tourism is facing
a market increasingly competitive to meet your needs in
the areas of hospitality and tourism in Ouro Preto and
Mariana. The numerous hotel establishments
continually suffer from a lack of skilled labor to work
mainly in camaria sector, which is occupied almost
exclusively by women. Objectives: To provide for the
market people trained to work as food handlers and
maids, training courses were given in Soares District, in
Reference Centers for Social Assistance São
Cristóvão, Engenheiro Corrêa and in Soares District
(Food Handlers) and CRAS Mariana and Antonio
Pereira (Chambermaids). Methodology: Lectures and
technical visits to lodging facilities were used in the
training of maids. For the course of food handlers, as
well as lessons and dynamic on food hygiene (ANVISA)
and homemade pasta production workshops, applying
the best practices, a practical lesson demonstrating the
issue of bacterial contamination, justifying the use of
31
caps and hand hygiene was also performed. Results:
In 2015 were trained 16 maids and 20 food handlers.
The technical part added with environmental education
content and entrepreneurship leads to women trained
full workouts, which allow them to enter the labor
market or set up associations and cooperatives.
Conclusions: The project contributes to the
improvement of food handling practices, often already
carried out by the students in their own homes; to
decrease the number of disease case foodborne and to
hand training of skilled labor in chambermaid service,
the which makes a difference because we are in a
tourist pole and the need for skilled labor in the means
of local accommodation is always checked.
Acknowledgements:
Pró-Reitoria de Extensão
Reference Centers for Social Assistance of Ouro Preto
and Mariana/MG
Financial Resources: Universidade Federal de Ouro
Preto
42
WORKSHOP OF ENVIRONMENTAL EDUCATION
AND WATER QUALITY MONITORING
Danielle Murta da Silva; Ícaro Mateus Silva; Samira
Fagundes de Andrade; Eduardo Antunes Almeida;
Aline Fonseca Bornachi; Beatriz Tonhela Rocha Araújo;
Vera Lúcia de Miranda Guarda;
Nucleus of the UNESCO Chair: water, women and
development.
Introduction: Environmental education aims at training
people to exercise responsible and conscious
citizenship, transforming and improving the relationship
between human beings and of these with the
environment in which they live. The study of water is
one of the most important subjects of study of
environmental education, because of its economic and
social value and the water crisis that overwhelms the
planet. Water for home and hospital use must have
constant monitoring in order to prevent waterborne
diseases that can lead to death children and the elderly.
The lack of drinkability is considered a serious public
health problem. Objectives: The Environmental
education workshops are intended to complement the
training of the training courses of the UNESCO Chair:
Water, women and development. In order to provide the
course participants the importance of responsible use
of natural resources, avoiding waste, generating
income and highlighting the problems resulting from
compulsive and predatory actions. The second
objective of the project is the monitoring of water
quality, which helps to reduce borne diseases by
consumption of contaminated water and inform the
public of the risks of contamination, also seeking to
raise awareness for the preservation of this good.
Methodology: environmental education workshops
were held in the UNESCO Chair of courses. The
workshops were composed of lectures and practices,
addressing we have: Environment (perception and
impacts); Water (consumption awareness and
waterborne diseases); Solid Waste (recycling and
composting); Gender (women's role in water
governance); Protection of springs; Care wells and food
security. To carry out quality monitoring, bacteriological
parameters were analyzed in several samples using the
method defined Chromogenic substrate ONPG-MUG
trademark Colilert. Results: With regard to
environmental education, the 36 students from various
training of the Chair were very receptive and attentive,
especially in practical classes, when debates on the
use of waste as tetrapark box and cooking oil come into
play, or in building a box composting. Monitoring,
samples were from the rural district of Santa Rita (water
springs); Of Saramenha at the request of the
Prosecutor and the Hospital. Most of these samples are
found contaminated by fecal coliforms demonstrated by
fluorescence under ultraviolet light. Conclusion:
Environmental Education complies with of the UNESCO
Chair of the prerogative of awareness and care for the
environment and especially water. It notes the
existence of non-potable water consumption.
Acknowledgements:
Pró-Reitoria de Extensão.
Reference Centers for Social Assistance of Ouro Preto
and Mariana/MG.
Financial Resources: Universidade Federal de Ouro
Preto.
43
UNESCO CHAIR ON WATER, WOMEN AND
DEVELOPMENT
Thais Santos Rodrigues; Samira Fagundes de
Andrade; Ícaro Mateus Silva; Danielle Murta Silva;
Beatriz Tonhela Araújo; Aline Fonseca Bornachi;
Amanda Maria Silva Gonçalves;
Fagner Parreiras Egg; Isadora Iannini Cota Dutra;
Thaís Pinto da Rocha Torres; Luiz Fernando de
Medeiros Teixeira; Ângela Leão Andrade; Fábio Viana
de Moura; Carolina Maranhão Albuquerque; Kerley dos
Santos Alves; Tays Torres Ribeiro das Chagas; Vera
Lúcia de Miranda Guarda.
Nucleus of the UNESCO Chair: water, women and
development.
32
Introduction: The mission of the UNESCO Chair on
Water, Women and Development in Brazil is to
encourage the construction of a sustainable society
through environmental education and empowerment of
women. The chair actively Works towards becoming a
Center of Excellence for preserving water resources,
promoting sustainable development and encouraging
women’s participation in all water-related activities. The
chair undertakes its projects through teamwork with
individuals from multiple fields and disciplines,
volunteer services and networking with diferente
parteners. Aims: The Chair primarily aims to creat
synergies between local actors and projects already
being undertaken in this area of focus, in order to
enhancetheir integration and sustentability. Other major
objectives include the promotion of education of women
in all areas related to water, enhacing women’s
entrepreneurial skills, and encouraging women to invest
in themselves and achieve decision-making power on
equal terms with men. In addition, the Chair also seeks
to train and qualify men and women as conscious
peers, striving to live ina balanced and sustainable
environment. Methods; The project reaches women in
conditions of social vulnerability in the city of Ouro
Preto and Mariana and promotes, through the Program
of Permanent Capacity, qualification courses in the
areas of hospitality, food and health. Not only qualify
them professionally, but also encourage to build their
own business are prospects for future plans in the
program of social entrepreneurship courses and
enviromental education . This women in the training
programme has two 20-hour modules. The first
focuses on gender and water issues while the second
targets entrepreneurial skills, to aid in assembly of
associations or cooperatives. Results: Classes and
dynamic in training courses in the areas of Crafts;
Hygiene, Maids; Food handler; Receptionists and used
oil soap production reached about 200 women and
many of them are employed in Ouro Preto hostels and
restaurants. Conclusion: Among the partners are the
companies Vale and Samarco, City Council of Ouro
Preto, Reference Center for Social Assistance (CRAS),
Gorceix Foundation. And internationals: Hydrological
International Programme of UNESCO for Latin America
and the Caribbean, Hidroex Institute: Foundation
International Center of Education, Capacity and Applied
Research in Waters of the Government of Minas and
Network of Chairs on Gender. Recently, the core of the
Chair has signed a collaboration agreement with the
Dominican Republic, through the National Institute of
Hydraulic Resources. All this partners helps to improve
women's quality of life and their families, where
women's empowerment has immediate impact.
Acknowledgement: International Hydrological
Programme, Pró-Reitoria de Extensão, Federal
University of Ouro Preto.
Financial resources: Federal University of Ouro Preto.
44
ANTIOXIDANT AND ANTI-INFLAMMATORY
ACTIVITIES OF ETHANOLIC AND AQUEOUS
EXTRACTS OF THE LEAVES FROM Tabebuia
roseo-alba.
Zilma S. Ferraz-Filha1, Iális P. A. R. Dutra
1, Fernanda
C. Ferrari1, Marcela C. P. Michel
1, Dênia A. Saúde-
Guimarães1.
1Laboratório de Plantas Medicinais – CiPharma -
Escola de Farmácia – Universidade Federal de Ouro
Preto – Ouro Preto – MG
Tabebuia species have several uses in folk medicine,
including their use in inflammation and rheumatism.
Tabebuia roseo-alba (Ridl.) Sandwith (Bignoniaceae) is
popularly known as “white ipe”. This study evaluated in
vitro antioxidant and in vivo anti-inflammatory activities
of ethanolic and aqueous extracts of leaves from T.
roseo-alba. Leaves powder was extracted by
percolation with ethanol and water, separately. Ethanol
was removed under reduced pressure to afford dried
ethanolic extract. Water was removed by lyophilization
to afford dried aqueous extract. These extracts were
evaluated in an experimental model of gouty arthritis at
doses of 125, 250 and 500 mg/kg, using monosodium
urate crystal-induced paw oedema, in Swiss mice.
Indomethacin was used as positive control. The
measurement of the paws of mice was made with a
caliper at times of 4, 24, 48 and 72 h after
administration of the extracts and controls. The paw
volume of monosodium urate crystal-induced mice
revealed an increase in paw diameter, whereas
indometacin (3 mg/kg) caused significant anti-
inflammatory activity with reduction of edema in 4, 24,
48 and 72 h. Treatments with both extracts, in all
evaluated doses, were capable of promoting a
significant reduction of paw oedema of the animals,
demonstrating the anti-inflammatory activity. Evaluation
of antioxidant activity was performed in vitro using three
methodologies: Capture of free radicals 2,2-diphenyl-2-
picrylhydrazyl (DPPH), azinobis 2,2-(3-
ethylbenzthiazoline-6-sulfonic acid) (ABTS) and by β-
carotene/linoleic acid system. Ethanolic and aqueous
extracts showed antioxidant activity of 58.14; 51.48 %
(DPPH/100 µg/mL); 74.43; 87.77 % (ABTS/40 µg/mL)
and 76.75; 81.28 % (β-carotene/linoleic acid/40 µg/mL),
respectively. Trolox and quercetin, in different
concentrations depending on the methodology, were
used as positive controls. The results showed that the
ethanolic and aqueous extracts of leaves from T. roseo-
alba showed promising antioxidant and anti-
inflammatory properties and may contain bioactive