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Abstract Book
State level Conference on ‘Microbiology in 21st Century’
25th – 26th February, 2011
Dr. Gajanan R. Ekbote Chief Organizer and
Chairman, Business Council, Progressive Education Society, Pune 5.
Dr. Rajendra. S. Zunjarrao Convener and
Principal, Modern College of Arts, Science and Commerce, Pune 5.
Dr. (Mrs.) Shilpa S. Mujumdar Organizing Secretary and Head, Department of Microbiology
Organized By
Department of Microbiology
Modern College of Arts, Science and Commerce Shivajinagar, Pune 5.
Under Quality Improvement Programme of University of Pune
State Level Conference on ‘Microbology in 21st Century’
II
Mission statement
“To Create
Multidisciplinary Best Citizens To Suit
Local, National and International Needs Having
Scientific Temperament, Moral and Ethical Values
and Multifaceted, Proactive Personality
By Providing Excellent Education”
1
State Level Conference on ‘Microbiology in 21st Century’
25th – 26th February, 2011 Organized by
Department of Microbiology
Modern College of Arts, Science & Commerce, Shivajinagar, Pune, 411005, Maharashtra, India.
Conference Schedule - Friday, 25th
February 2011
Inaugural Function
10.00 am-11.00 am Registration and Refreshment
11.00 am-12.00 pm Inauguration
• DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Pune
• DR. RAMESH S. PARANJAPE, Director, NARI, Pune
• PROF. MRS. JYOTSNA. G. EKBOTE, Chairman, LMC (Junior College), MCASC, Pune
• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Pune
• DR. ARVIND K. PANDE, Prof-In-charge, Department of Biotechnology and Microbiology.
12.00 pm-12.45 pm DR. RAMESH S. PARANJAPE, Key Note Speaker
Director,
National AIDS Research Institute, Pune
12.45 pm-01.30 pm Lunch
Session I
Lectures
01.30 pm-02.00 pm DR. PRASHANT K. DHAKEPHALKAR, Chairperson
Scientist ‘E’, Agharkar Research Institute, Pune
02.00 pm-02.45 pm
DR. S. MOHAN KARUPPAYIL, Invited Speaker
Topic:” Prokaryotes and Eukaryotes: Do they talk to each other?”
Professor and Director, School of Life Sciences, S.R.T.M.University, Nanded
02.45 pm-03.30 pm
DR. PRAKASH R. THORAT, Invited Speaker
Topic:”Decolourization and degradation of textile dyes by microorganisms”
Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.
Oral Presentations
03.30 pm-03.40 pm
OP 01
Candida glabrata infection of the nasal septum – an unusual case report.
Dr. Roma A. Chougale
MBBS, MD, Lecturer in Microbiology, D. Y. Patil College, Kolhapur.
03.40 pm-03.50 pm
OP 02
Prevalence of methicillin resistant Staphylococcus aureus in D.Y.Patil hospital and Research centre.
Dr. Reena A.Dighe
MBBS, DCP, MD, Department of Microbiology,D. Y. Patil Medical College, Kolhapur.
03.50 pm-04.00 pm
OP 03
Bacteriological quality of water, sanitation interventions and health with special reference to diarrhoea.
Mr. Anil M. Garode
Department of Microbiology, Shri. Shivaji Science and Arts College, Chikhli, Buldhana.
04.00 pm-04.10 pm
OP 04
Screening of fungal isolates for lignolytic enzyme, manganese peroxides
Madhuri A. Udata, Joshi A. J., Dr. Mahendra K. Ranjekar.
Department of Microbiology, Dayanand Science College, Latur.
04.10 pm-04.20 pm
OP 05
Biotransformation of Textile Azo Dyes by Aerobic Bacteria.
Ravi V. Kale and Prakash R. Thorat.
P.G.Department of Microbiology and Research Center, Shri Shivaji Mahavidyalaya, Barshi, Solapur.
04.20 pm-04.30 pm
OP 06
A cost-effective protocol for cultivation, microbial and biochemical analysis and high yielding nutritious variety of
mushrooms.
Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P. Anandwani, Jayesh A. Patil, Department of Microbiology,
PSGVPM’s, ASC College, Shahada, 425409, Nandurbar.
04.30 pm-04.40 pm
OP 07
Biosurfactant production by a marine strain of Pseudomonas.
Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Prof. Balu A. Chopade.
Department of Microbiology & Institute of Bioinformatics and Biotechnology, University of Pune, Pune.
04.40 pm-05.00 pm Tea
2
Conference Schedule – Saturday, 26
th February 2011
Session II
Lectures
10.00 am-10.30 am Refreshment
10.30 am-11.00 am DR. DIGAMBAR V. GOKHALE, Chairperson
Scientist ‘F’, National Chemical Laboratory, Pune
11.00 am-11.45 am
PROF. ARVIND M. DESHMUKH, Invited Speaker
Topic:”Microbial solubilization of metals from waste printed circuit boards”
Head, Department of Microbiology, BAMU, Osmanabad.
11.45 am-12.30 pm
PROF. SUDHIR B. CHINCHOLKAR, Invited Speaker
Topic:”Recent Trends in Microbial Control of Phytopathogens”
Director, BCUD, North Maharashtra University, Jalgaon
Oral Presentations
12.30 pm-12.40 pm
OP 08
Antiplasmid activity of herbal extracts on Methicillin Resistant Staphylococcus aureus
Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar.
Department of Microbiology, K. T. H. M. College, Nashik
12.40 pm-12.50 pm
OP 09
Study of extended spectrum beta lactamase (ESBL) producing Gram negative bacilli in family Enterobacteriaceae.
Dr.Vishwashanti. S. Vatkar, Dr.P.G.Shadija, Dr.S.J.Ghosh
Department of Microbiology, Dr.D.Y.Patil Medical College, Kasaba Bavada, Kolhapur.
12.50 pm-01.00 pm
OP 10
Microbial Bioaugmentation for treatment of industrial effluents in Common Effluent Treatment Plant (CETP)
Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Dr.Seema S.Sarnaik, G.K.Wagh, Dr.Pradnya P.Kanekar
Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune.
01.00 pm-01.10 pm
OP 11
Isolation of Rhizobium and Optimization of Growth Conditions for Development of Biofertilizer for Trigonella
foenumgraecum (Fenugreek).
Aasawari S. Pawar and Rajendra Choure.
Department of Microbiology, The Institute of Science, Mumbai.
01.10 pm-01.20 pm
OP 12
Synthesis and characterization of ZnO nanoparticles and their application on textiles as antimicrobial agents and UV
absorbers.
Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija.
Department of Biotechnology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.
1.20 pm-01.30 pm
OP 13
Comparative study on the production of Prodigiosin by Serratia marcescens using various crude fatty acid sources and its
applications.
Pankaj .S. Picha, Sheetal .P. Pardeshi
Department of Microbiology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.
01.30 pm-02.00 pm Lunch
Session III
02.00 pm-03.00 pm Poster Presentation
Session IV
03.00 pm-04.00 pm
Panel Discussion
• DR. BALU A. CHOPADE, Director, Institute of Bioinformatics and Biotechnology, Pune
• DR. RAJNEESH B. VAIDYA, HOD, Microbiology, The Institute of Science, Mumbai.
• DR. BALU P. KAPADNIS, HOD, Microbiology, University of Pune, Pune.
• DR. RENU BHARADWAJ, Dean, B.J.Medical College, Pune.
• DR. PRAKASH R. THORAT, Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.
• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar, Pune.
• DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology
04.00 pm-04.10 pm Tea
04.10 pm-04.40 pm
Valedictory function
• DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Shivajinagar, Pune.
• DR. RAMCHANDRA V. GADRE, Scientist, National Chemical Laboratory, Pune
• PROF. MRS. JYOSTNA. G. EKBOTE, Chairman, LMC Junior College, MCASC, Shivajinagar, Pune.
• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar , Pune
• DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology, MCASC, Shivajinagar , Pune
• DR. SHILPA S. MUJUMDAR, Head, Department of Microbiology, MCASC, Shivajinagar, Pune.
04.40 pm-05.10 pm Certificate Distribution
Messag
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State Level Conference on ‘Microbology in 21st Century’
V
Message from Head, Microbiology, University of Pune
Professor B. P. Kapadnis Head, Department of Microbiology, University of Pune, Ganeshkhind, Pune 07
“I am happy to know that the Department of Microbiology, Modern College
of Arts, Science and Commerce, Pune 05, is organizing State Level Conference entitled “Microbiology in 21st Century” on 25th and 26th February 2011.
In today’s Modern world, Microbiology is considered a vibrant and exciting field with many applications and the new and exciting discoveries been made in microbial ecology, molecular genetics, space microbiology etc. With the changing faces of microbes, it is mandatory for us to refine our approaches towards treating the infectious diseases. Genetically tailored medicine is picking up pace and that really gives us a ray of hope in combating the problem of multidrug resistant organisms which is the need of the hour. This conference will present multitude of subjects with a unique blend of modern research in microbiology.
I am certain that this conference will prove to be the perfect platform for the professionals to greatly benefit from the exchange of ideas and also the debates and deliberations related to the explorations of an integrated approach to microbiology.
I wish all the best and success for this conference.” Professor B. P. Kapadnis Department of Microbiology, University of Pune, Ganeshkhind, Pune 07
Chairma
Dr. G. R. EChairmanBusiness CProgressivShivajinag Chief Org‘State leveMicrobiol25th - 26th
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Progressive edshtra. P. E. Soo Kanitkar alowith their unts over a periodrun by the sociall over Mahare-Primary, Primmerce Collegeent, Institute oge etc. he Modern Coed by Progressof Pune. The
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State Level Conference on ‘Microbology in 21st Century’
VII
noted the achievements and reputation of the college. The Govt. of Maharashtra and University of Pune has recently accorded the sanction to increase the intake capacity of the M.Sc. course. The young and highly qualified team of faculty members spares no efforts to keep the students well informed and in updating their knowledge. The students are also reciprocating by their high achievements in academic performance. A large number of students are getting excellent placements in different part of the country & also abroad. Every year several students are also selected in reputed foreign universities for higher studies. Concentrated research and development is required on various micro based technologies that can be utilized to increase crop production and utilize agro waste, manage abiotic stress drug design, for biocontrol of important insect pests and in post harvest technologies
I am certain that the conference will prove to the perfect platform for the professionals to greatly benefit from the exchange of ideas and also, the debates and deliberations related to explorations of integrated approach to Microbiology. I also hope that there will be sufficient knowledge and direction for the young and budding scientists also.
I congratulate the principal of modern college Dr. Rajendra S. Zunjarrao, Dr. Shilpa S. Mujumdar, HOD, Department of Microbiology and organizing team for organizing this conference and wish them a grand success.”
Dr. G. R. Ekbote Chairman, Business Council, Progressive Education Society Shivajinagr, Pune 5.
Principa
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State Level Conference on ‘Microbology in 21st Century’
IX
science. With this notion in mind we had submitted proposal to the University of Pune to organize State level conference on ‘Microbiology in 21st Century’ under its Quality Improvement Programme. We are very glad to communicate you that, in response to our Proposal University of Pune has given approval and consent to organize the International Conference on the said topic.
As it is state level event we have invited and expecting the participation of Resource Persons, Subject Experts, Scientists, Eminent Personalities from the academic field and industry, Faculties and Students from the various districts in Maharashtra. On this occasion I convey my warm greetings and felicitations to all the delegates and wish them a scintillating conference.”
Dr. Rajendra S. Zunjarrao Principal, Progressive Education Society’s Modern College of Arts, Science and Commerce, Shivajinagar, Pune 5.
State Level Conference on ‘Microbology in 21st Century’
X
Committees for State Level Conference “Microbiology in 21st Century” 25th and 26th February 2011
Organizing committee Dr. G. R. Ekbote, Chief Organizer and Chairman, P.E. Society.
Dr. R. S. Zunjarrao, Principal and Convener
Prof. A. G. Gosavi, Visitor, MCASC, Pune 5
Dr. A. K. Pande, Prof. In‐Charge, Microbiology
Dr. (Mrs.) S. S. Mujumdar, Organizing Secretary and HOD, Microbiology
Prof. A. M. Bhalerao, Professor, Dept. of Zoology
Prof. S. S. Thengadi, Professor, Dept. of Physics.
Prof. Y. Thipse, Asst. Prof. Dept. of Electronics
Ms. S. S. Bhuyan, Asst. Prof, Microbiology
Mr. V. N. Bobade, Asst. Prof., Microbiology
Ms. S. P. Pardeshi, Asst. Prof., Microbiology
Ms. S. P. Bashetti, Asst. Prof, Microbiology
Mrs. A. A. Devale, Asst. Prof., Microbiology
Ms. M. J. Amrutkar, Asst. Prof, Microbiology
Mrs. R. S. Sawant, Asst. Prof, Microbiology
Advisory Committee Dr. G. R. Ekbote, Chairman, P.E. Society.
Dr. P.S. Chirputkar, Chairman, LMC (Senior College), MCASC.
Dr. A. K. Pande, Prof.‐In‐Charge, Dept. of Biotechnology and Microbiology, MCASC, Pune 5.
Prof. Mrs. J. G. Ekbote, Chairperson, LMC (Junior College), MCASC.
Dr. R. S. Zunjarrao, Principal, MCASC, Pune 5.
Prof. A. G. Gosavi, Visitor, MCASC, Pune 5.
Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 5.
Prof. S. Choudhari, Vice‐Principal, MCASC, Pune 5
State Level Conference on ‘Microbology in 21st Century’
XI
Scientific Advisory Committee Prof. Dr. B. A. Chopade, Director, IBB, University of Pune
Prof. Dr. B. P. Kapdnis, Head, Dept. of Microbiology, University of Pune.
Prof. Dr. R. L. Deopurkar, Professor, Dept. of Microbiology, University of Pune.
Dr. P. K. Dhakephalkar, Scientist ‘E’, ARI, Pune
Prof. Dr. Sankunny M. Karuppayil, Director, School of Life Sciences, S. R. T. M.
University, Nanded
Dr. D. V. Gokhale, Scientist ‘F’, Scientist In charge, ICIM, NCL, Pune
Prof. A. M. Deshmukh, Head, Department of Microbiology, BAMU, Osmanabad
Prof. S. B. Chincholkar, Director, BCUD, North Maharashtra University, Jalgaon
Dr. P. R. Thorat, Head, Dept. of Microbiology, Shri Shivaji Mahavidyalay, Barshi
Dr. H. V. Ghate, Head, Dept. of Zoology, MCASC.
Editorial Committee Chief Editor: Dr. R. S. Zunjarrao, Principal
Advisor: Dr. A. K. Pande
Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 05.
Members: Dr. (Mrs.) S. S. Mujumdar
Mr. V. N. Bobade
Ms. S. S. Bhuyan
Travel and Accommodation Committee Advisor: Prof. A. G. Gosavi, Visitor
Members: Dr. (Mrs.) S. S. Mujumdar
Mr. V. N. Bobade
Mr. P. S. Warhade
Mr. A. A. katkar
Mr. V. M. Daundkar
Ms M. J. Amrutkar
Mr. P. Sabale
State Level Conference on ‘Microbology in 21st Century’
XII
Technical Assistance Committee Advisor: Prof. S S. Deshmukh
Prof. A. Sathe,
Members: Mr. V. N. Bobade
Ms. S. P. Pardeshi
Ms. Gitanjali Litake
Ms. A. V. Jagdale
Mr. A. A. Katkar
Mr. V. M. Daundkar
Mrs. J. J. Dunde
Mr. R. Shawalkar
Mr. Manik Jadhav
Registration Committee Advisor: Mr. R. P. Kale, Registrar
Members: Mrs. A. A. Devale
Ms. S. P. Bashetti
Mrs. R. S. Sawant
Mr. R. Dabhade
Ms. R. Thombare
Mr. V. Lad
Mrs. S. Patwardhan
Mr. V. M. Daundkar
Ms. A. V. Jagdale
Mr. Sagar Kudale
State Level Conference on ‘Microbology in 21st Century’
XIII
Finance and Sponsorship Committee Advisor: Prof. S. S. Thengdi
Members: Mrs. A. A. Devale
Ms. S. S. Bhuyan
Ms. M. J. Amrutkar
Mrs. S. Puranik
Mr. D. Shah
Mr. A. Kakad
Certificate Preparation Committee Advisor: Prof. R. N. Ingole
Members: Mrs. A. A. Devale
Ms. S. P. Bashetti
Mr. R. Dhabade
Mrs. R. Thombare
Hall Arrangement Committee Advisor: Prof. S. S. Deshmukh
Members: Dr. (Mrs.) S. S. Mujumdar Mrs. Ranjana Dhotre
Ms. S. S. Bhuyan Mr. Tushar Bharekar
Ms. M. J. Amrutkar
Ms. S. P. Pardeshi
Mrs. A. Sardesai
Ms. A. V. Jagdale
Mr. A. A. Katkar
Mr. V. M. Daundkar
Mrs. J. J. Dunde
Mr. R. Shawalkar
Mr. Prasad Vanage
Mr. Rajendra Khapre
Mr. Sameer Dhavade
State Level Conference on ‘Microbology in 21st Century’
XIV
Hospitality and Food Arrangement Committee Advisor: Prof. Y. Thipse
Members: Mrs. A. A. Devale
Ms. M. J. Amrutkar
Mrs. A. Sardesai
Mr. A. Kakad
Mrs. R. S. Sawant
Ms. S. P. Bashetti
Mr. P. Sabale
Publicity Committee Advisor: Dr. A. K. Pande
Members: Dr. (Mrs.) S. S. Mujumdar
Ms. S. P. Pardeshi
Ms. S. P. Bashetti
Session In‐Charge Advisor: Dr. (Mrs.) Shilpa S. Mujumdar
Members: Ms. S. P. Pardeshi
State Level Conference on ‘Microbology in 21st Century’
XV
Volunteers
Aniket Hambir Khude Rasika R.
Anuradha Kulkarni Konde Pallavi
Baviskar Ravindra Dhondu Lande lalit Satyawan
Bedare Monika Kundalik Londhe Madhuri Hanumant
Bhong Yogita D. Malla Shikha
Bhoodatt Mrinmai R. Mandal Mamta Rajendra
Bidkar Monali A Mane Deepali Sahebrao
Chaudhari Saurabh Renukadas Mane Priyanka Vasant
Chhajed Khushbu V. Mehendaley Saili Vishwas
Chothe Nital Syam Nadivkar Pradnya Nandkumar
Churi Ruta K. Naina Gaikwad
Daithankar Bhagyashree D. Mazhar Tahaseen
Dasarwar Shriniwas Vijaykumar Pallavi Konde
Deore Poonam Vasant Patil Datta V.
Deshmukh Sujit M. Patil Pratibha Abhimanyu
Deshpande Manisha M Pawar Gauri Manohar
Dhamal Hrishikesh Dilip Picha Pankaj Sudhirkumar
Gaikwad Sarika Punde Priya S.
Gaikwad Sunny Tanaji Raut Tushar C.
Gavhane Supriya S. Ravindra Baviskar
Ghole Tushar S. Rohani Neha Giridhar
Inamdar Bhagyashree Anant Sakhare Reshma Ramakant
Jade Rupali S. Sanam Vynkatesh Madhukar
Jadhav Amar Dnyaneshwar Sen Divya Deepakkumar
Jadhav Kanchan Janardan Sheetal Joshi
Jadhav Shweta M. Shelar Akshay B.
Jere Saee S. Shetty Rashmi Deepak
Kadam Monika A. Shrinivas Dasarwar
Kale Trupti P. Singh Ajay Pal
Kalel Preeti V. Sujata Shinde
Kanchan Jadhav Telore Snehal Vilas
Kasyap Poonam R. Thorave Varsha J.
Kamelia Behroozi Jagtap Bhagyashree
Tejaswita Pawar Phulphagar Akshata
Yadav Priya Manisha Shelar
Shelar Prachi Madhuri Wadkar
Reshma Yeole
State Level Conference on ‘Microbology in 21st Century’
XVI
Contents – Oral Presentations Abstracts Title and Authors Page
OP1 Candida glabrata Infection of The Nasal Septum – An Unusual Case Report Roma A. Chougale
57
OP2 Prevalence of Methicillin Resistant Staphylococcus aureus in D. Y. Patil Hospital And Research Centre Reena Dighe and Saral Ghosh
54
OP3 Bacteriological Quality of Water, Sanitation Interventions And Health With Special Reference To Diarrhoea. Anil M. Garode
06
OP4 Screening of fungal isolates for lignolytic enzyme, manganese peroxides Madhuri A. Udata, Joshi A. J., Mahendra K. Ranjekar
74
OP5 Biotransformation of Textile Azo Dyes by Aerobic Bacteria. Ravi V. Kale and Prakash R. Thorat
26
OP6 A cost-effective protocol for cultivation, microbial and biochemical analysis and high yielding nutritious variety of mushrooms. Jogi. M. M., Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P. Anandwani, Jayesh A. Patil
25
OP7 Biosurfactant production by a marine strain of Pseudomonas. Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Balu A. Chopade
81
OP8 Antiplasmid activity of herbal extracts on Methicillin Resistant Staphylococcus aureus Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar
11
OP9 Study of extended spectrum beta lactamase (ESBL) producing Gram negative bacilli in family Enterobacteriaceae. Vishwashanti. S. Vatkar, P.G.Shadija, S.J.Ghosh
79
OP10 Microbial Bioaugmentation for treatment of industrial effluents in Common Effluent Treatment Plant (CETP) Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Seema S. Sarnaik, G. K. Wagh, Pradnya P. Kanekar
63
OP11 Isolation of Rhizobium and Optimization of Growth Conditions for Development of Biofertilizer for Trigonella foenumgraecum (Fenugreek) Aasawari S. Pawar and Rajendra Choure
01
OP12 Synthesis and characterization of ZnO nanoparticles and their application on textiles as antimicrobial agents and UV absorbers. Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija
56
OP13 Comparative study on the production of Prodigiosin by Serratia marcescens using various crude fatty acid sources and its applications Pankaj .S. Picha, Sheetal .P. Pardeshi
45
State Level Conference on ‘Microbology in 21st Century’
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Contents – Poster Presentations Abstracts Title and Authors Page PP1 Isolation and Characterization of Listeria species from
environmental and clinical sample Abhay Raorane & Barbuddhe S.B.
02
PP2 Antimicrobial activity of Vinca rosea (Catharanthus roseus L.) and Clematis gouriana against pathogenic bacteria Akshay Borkar, Manoj Bhosale, Shirish Shah, Salokhe S.G., Sonawane H.V
03
PP3 Isolation and Screening of plant growth promoting bacteria from rhizosphere Alka Jagdale,Shradha Bashetti, Smita Bhuyan, Shilpa Mujumdar, Anushka Devale
04
PP4 Isolation of Poly-β-Hydroxyalkanoate Producing Bacteria from waste water and optimization of its production parameters Amar D. Jadhao, Hrishikesh D. Dhamal, Vivek N. Bobade
05
PP5 Characterization of contaminants of cosmetics and their antibacterial sensitivity against synthetic and natural preservatives. Ayushi.A.Chaurasiya And R.B.Vaidya
07
PP6 Study of quorum sensing and quorum quenching in Vibrio fischeri and application in aquaculture Archana J. Marry & Jyoti Mantri
08
PP7 Biodegradation of Triaminotrinitrobenzene (TATB) Abhijit More, Sayali Mahajani, Seema Sarnaik & Pradnya Kanekar
09
PP8 Production of amylase by bacteria isolated from soil sample Bedare Monika & Madhuri Amrutkar
10
PP9 Characterization of thermo-tolerant and acid /alkali tolerant β glucosidase from bacterial isolate Bhalkikar S.B. , Diwan V.A., Ranjekar M.K
12
PP10 Detoxication of Azo Dyes by microbial pure cultures as well as microbial consortia. Bholay A.D., Maid Sneha A., Borse Priti k., Rakte Snehal B
13
PP11 Probiotic properties of Lactobacillus isolates from milk of domestic animals and Commercial available probiotic preparations against enteric bacterial pathogens Bhutada SA & Tambekar DH
14
PP12 Isolation , purification & application of lipase enzyme. Bhadale S. V, Bhalerao M.N, Patil N.N, Ghule P.V & Kulkarni
15
PP13 Antibiotic Production from Bacillus spp. Active against Staphylococcus aureus Chavan K.J, Patil S.D, Ranjekar M.K.
16
PP14 Antimicrobial activity of mangroves against multidrug-resistant (MDR) pathogens. Bholay.A.D, Kurup Sunila .B., Vidhate Pushpa.S., Rahatekar Rohini .R.
17
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PP15 Biosorption of hexavalent chromium from aqueous solutions using dead fungal biomass of P. chrysogenum Deep.V.Barot & Varsha.Vaidya
18
PP16 Study of antibiotic resistance patterns in Campylobacter spp. isolated from Pune, Maharashtra De Souza NA, Kamble A, Parkar SD, Kapadnis BP
19
PP17 Antimicrobial Activity of new Sulphanylamide Analogues against Commonly occurring Multidrug Resistant Urinary Tract Pathogens Gauri Devasthale, Alok Vyas, Divya Nair & Jhilmil Ghoshal
20
PP18 Isolation, identification and characterization of bioluminescent bacteria from fish sample and its use as water pollution indicator Gauri Pawar, Deepali Mane, Vyankatesh M. Sanam, Shilpa Mujumdar & Smita Bhuyan
21
PP19 Assessment of genetic diversity among mango varieties by RAPD markers Hrishikesh Joshi, A. R. Desai
22
PP20 Development of microbial biostimulants to enhance fertility of saline soils for sustainable agriculture Indulkar V.S. Siddiqui A.G., Ranjekar M.K
23
PP21 Assessment of Water Purification Potential of Moringa Oleifera Coagulant Protein (MOCP) Jain Reena R & Kadam Shradhha D
24
PP22 Isolation of Bacteriophage against Campylobacter from Poultry in Pune Kamble A, De Souza N, Parkar S, Kapadnis BP
27
PP23 Isolation and Identification of Pathogens of Seafood Penaeus sp. Ketki Nalawade & Rajendra Choure
28
PP24 To study anti-bacterial activity of various medicinal plants against mixed dental flora Khadpekar A & Thombre R.
29
PP25 Screening & Production of Laccase from Fungi Kawade S.S, Diwan V.A., Ranjekar M.K
30
PP26 Studies on bioemulsifier production from Provdentia stuartii isolated from garden soil. Lalit Lande, Smita Bhuyan & Shilpa Mujumdar
31
PP27 Comparative study of bioinoculants prepared from rhizosphere soil and non-rhizosphere soil. M. S. Vrushali ,N.Rutika
32
PP28 Isolation of siderophore producing organism from rhizosphere soil of Adathoda vasica Madhuri Londhe, Smita Bhuyan,Shilpa Mujumdar
33
PP29 Comparative Analysis of Faecal Microbiota from Normal and Obese Wistar rats. Mahesh R Ghule,Rajesh M Chomal ,Deepak R Patil & Yogesh S Shouche
34
State Level Conference on ‘Microbology in 21st Century’
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PP30 Production of bacterial cellulose by Acetobacter xylinum (NCIM 2526) using fruit waste and checking for its application Manasi Karmarkar, Poorva Dabir & Meghana Kukarni
35
PP31 Aureobasidium Pullulans Grown On Various Feedstocks For Comparative Studies Meenakshi Dhar, Nasim Kherad, Vinay Rale
36
PP32 Antimicrobial effect of bombax ceiba Monika More, & Gauri Jadhav
37
PP33 Production and purification of extracellular RNases from Streptomyces venezualae (NCIM 2215). Mokshada Godbole, Neelima Kulkarni & S. S. Deshmukh
38
PP34 Isolation screening for penicillin- v acylase producing organism optimization of culture conditions, effect of media constituents on production of enzyme from B. cereus Neha Naik, Avinash Sunder, Laxmi Nair, Vaidehi Dande & A.V. Pundle
39
PP35 Characterization of plasmid encoded resistance to β-lactam antibiotics of Acinetobacter baumannii AIIMS7, a clinical isolate Neha S. Vora, Shradha B. Nadhe, Riddhi J. Shah & Karishma R. Pardesi
40
PP36 Optimization of growth parameters for ethanol production by yeasts Neelima Kulkarni, Shreyas Kumbhare, Pandurang Lahare, Hussain Khokhawala
41
PP37 Comparative In-vitro Antimicrobial activity of the extract of Gloriosa superba against laboratory and antibiotic resistant clinical isolates of common pathogens Nikita L. Lad and Rajendra G. Choure
42
PP38 Extracellular RNase production and purification from Streptomyces sp. (NCIM 2081) Niruta Y. Killedar, Anupama Sonawane and Sumedh S. Deshmukh
43
PP39 Isolation and characterization of oxalate degrading organism P.S, Patil , Patil Sham A , Bedse,Tanuja D, Jadhav Sandip R .
44
PP40 Oligotyping of antibiotic resistance genes from clinical isolates of Acinetobacter spp. Poojari Pritika, Ratnakaran Neena, Mehta Khyati, Shirode Abhinav, Pardesi Karishma
46
PP41 Optimization of Amylase, production by fungi isolated from rhizosphere soil. Pradnya N. Nadivkar & Sheetal. P. Pardeshi
47
PP42 Reduction of heavy metals from domestic sewage by using Microorganisms Prajakta Bhagwat, Priya Bendre and Vrushali Patil.
48
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PP43 Isolation and characterization of indole acetic acid (iaa) producing organism from rhizosphere of gram (Cicer arientinum). Priyanka Mane, Smita Bhuyan & Dr. Shilpa Mujumdar
49
PP44 Wound Healing Properties of honey. Purnima Ranawat & Jyoti Mantri
50
PP45 Optimization and characterization of lipase production by a selected Bacillus sp. and its application Rahul Brid and Rajendra Choure
51
PP46 Laboratory Study on Bioremediation of Diesel Oil Contaminated Soil from a Garage Rakhi Tari & Shilpa Sabnis
52
PP47 Production of giberrelic acid by soil fungi Ravindra Baviskar, Sunny Gaikwad, Shilpa Mujumdar & Smita Bhuyan
53
PP48 Production of Xanthan gum by Xanthomonas campestris using Industrial and kitchen waste as a production media Repe A.B., Dhutekar S.S,Ranjekar M.K.
55
PP49 Biological synthesis of silver nanoparticles and evaluation of their antibacterial activity Sabah Shaikh & Shilpa Sabnis
58
PP50 Study of ocular microflora, antimicrobial and antiadhesive activity of MPS solutions on ocular isolates. Sagar Vandra & Shilpa Sabnis
59
PP51 Identification and extraction of tobacco mosaic virus (DNA) using molecular techniques Samriddhi R. Sharma & Aparna Das
60
PP52 Phylogenetic analysis of insulin (protein) using bioinformatisc tools Sandeep Gajbhiye, Mr. Sunakar
61
PP53 Microbial degradation of Diaminodinitroethene, a high energy nitroexplosive Sayali Mahajani,Abhijit More, Seema Sarnaik & Pradnya Kanekar
62
PP54 Determination Of Resistance Of Listeria monocytogens Towards Sanitizers And Antibiotics In Presence Of Tween 80 Shahina Shaikh, Abhay Raorane & Deepali Giremar
64
PP55 Antibacterial activity of crude extracts of spices against food borne pathogenic microorganisms. Shikha Malla; Shilpa Mujumdar; Madhuri Amrutkar
65
PP56 Evaluation of antibacterial activity of pigments produced by Pseudomonas aeruginosa Shilpi.R.Chand & R.B.Vaidya
66
PP57 Biodegradation of Hexavalent Chromium Using Microorganisms Singh Viveka , Srivastava Shreya and Gore Suneeti
67
PP58 Study of Synthesis of Silver nanoparticles. Sourabh Mehta, Rebecca Thombre, Shubhangi Puranik.
68
State Level Conference on ‘Microbology in 21st Century’
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PP59 Decolorization of textile dye direct lemon yellow by Burkholderia cepacia Sunil. S. Tiwari & Sangeeta. K. Jangam
69
PP60 Phytochemical and antibacterial screening of solvent extracts of leaves of Bauhinia racemosa Lam. (Caesalpiniaceae) against enteric bacterial pathogens S.B.Dahikar, S. A. Bhutada, D.H.Tambekar, and S. B. Kasture
70
PP61 Viral Diagnosis by using RT – PCR: A case study on Banana Chlorosis Disease S.O. Gupta & B.A. Aglave
71
PP62 Biopulp - an eco-friendly approach S. M. Kulkarni, Navale S. K.,Sulkshane H. C.,Sharma L. P., Khandelwal S.R., Shirke M. D., Kokani N. F., Bhavsar S. P., &. Joshi S. A.
72
PP63 Plasmid Profiling and Antibacterial Activity Of Garlic Extract And Clove Oil On Clinical Isolates Of Pseudomonas aeruginosa Tejlaxmi Inamdar, Milky Sahukar, Kavita Parekh.
73
PP64 Screening of Lipases as potential virulence factor in Acinetobacter species isolated from different hospitals in India. Urvi Doshi & Karishma Pardesi
75
PP65 In Vitro Studies of Antimicrobial Properties of Extracts from Unani Medicinal Plants Vaishali Prabhune, Namra Ghansar, Supriya Bhopale
76
PP66 Lignin degrading peroxidase in biobleaching of paper-pulp mill and textile dye-based effluents V. A. Tile, Borkhataria B. V, Bhalerao V. B, Aher N. S.
77
PP67 Bacterial synthesis of silver nanoparticles and its applications V.A.Tile., S.P.Pawar., W.R.Sayyed., A.V.Bhosale
78
PP68 Accumulation of polyhydroxyalkanoate(PHA) from styrene by Pseudomonas spp and effect of N2 concentraction on PHA accumulation. Vidya N. Udawant, Smita A. Daule & D. D. Giramkar
80
PP69 Utilization of molasses for biosurfactant production by Streptomyces S2 Vynkatesh. M. Sanam, Deepali S. Mane., Gauri M. Pawar, Shilpa. S. Mujumdar & Smita. S. Bhuyan
82
PP70 Isolation, Characterization and Application of Phenol Degraders Uzma T. Momin and Varsha K. Vaidya
83
PP71 Antibacterial Activity of Medicinal Plants against Gastrointestinal Pathogens Pratibha A Patil , Shilpa S Mujumdar, Madhuri Amrutkar.
84
PP72 Utilization of petroleum hydrocarbons by bacteria isolated from soil & water contaminated with engine oil Dalvi, C. S., Satbhai, M. A., Bhutada, S. A.
85
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PP73 Biodegradation of commercially used insecticides by soil isolates B. A. Karode, N. B. Choudhari, S. S. Patil, P.T. Patil & A. H. Jobanputra
86
PP74 Biodegradation of diesel oil by soil isolates V. R. Kothari & A. H. Jobanputra
87
PP75 Raw wastes as alternative media for Trichoderma: Towards sustainable development Rushikesh Sankpal, Sunil Deshmukh & Vinay Rale
88
PP76 Characterization and biodiversity of Pashan lake water Rahul Nimbalkar & Varsha Kulkarni
89
State Level Conference on ‘Microbology in 21st Century’
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Extended Abstract
Prokaryotes and Eukaryotes: Do They Talk to Each Other?
Prof. S. Mohan Karuppayil
School of Life Sciences, SRTM University, Nanded. 431606
A paradigm shift is happening in our understanding of the world of unicellular eukaryotes and prokaryotes. The microorganisms, like bacteria and yeasts are often considered as non cooperative individualistic organisms. In fact, they are very social organisms and in nature they tend to live as communities called as biofilms and often behave like multicellular organisms. Biofilms are multicellular assemblages of cells encased in extracellular matrixes. They are interactive and use small diffusible molecules for communication, through a process referred as quorum sensing. They release small diffusible molecules which parallel with cell density and bind to sensors when they reach a critical concentration. The bound proteins activate the transcription of various genes resulting in behavioral changes. Hundreds of genes are regulated by the quorum sensing or chemical signaling molecules. The genes regulated include those which govern luminance, pathogencity, virulence factors, symbiosis/predation to cite a few. The chemical language used by microorganisms may be species/ genera specific or general. In short, microorganisms can detect the presence and density of not only their own species, but also members of other genera or even kingdom. Most of the time, the biofilms are not homogenous assemblages but polymicrobial. For example, in cystic fibrosis patients Pseudomonas aeruginosa-Candida albicans biofilms are common. Various studies shown that bacteria and fungi are not silent partners in biofilms but are engaged in meaningful dialogues resulting in changes in gene expression and observable behavioral changes. Eukaryotic hosts may recognize the quorum sensing molecules produced by the pathogens and may adapt strategies to handle it. Quorum sensing molecules of the pathogens are shown to elicit responses in the host often in favor of them or vice versa. The prokaryote - eukaryote dialogue is not only of importance to pathogenecity or virulence but may also influence secondary metabolite production, symbiosis and/or predation. Understanding the prokaryote-eukaryote dialogue may open up new opportunities for drug development and also reveal the marvelous biology behind the interactions.
State Level Conference on ‘Microbology in 21st Century’
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Extended Abstract
Decolorization and Degradation of Textile Azo Dyes by Microorganisms
Dr. P. R. Thorat
[email protected] Department of Microbiology and Research Center, Shri. Shivaji Mahavidyalaya,
Barshi – 413411, Dist. Solapur, Maharashtra
Water is the most precious divine natural resource that exists on our planet. Environment and life are in very close relationship on earth. Humans must protect these natural resources to lead life happily, but rapid industrial development and population explosion has made the problem of environmental pollution more and more acute. In India maximum population inhabits in villages and slum areas, where there is no safe drinking water supply. Dyes and pigments are used in various industrial processes like, textile, pharmaceutical, cosmetics, food, printing, etc. The textile industry is the largest, indispensable and backbone industries of some under-developed and developing countries in the world. The water required for textile industry is very large. Azo dyes that are used in the textile industries are xenobiotic compounds which contain (-N=N-) linkage and which are mutagenic as well as toxic to living organisms and environment also. Textile industry is confronted with the problem of color removal and effluent salt content reduction. The most pressing environmental problem facing the textile industry is the dye containing waste-water. The dye containing effluent represents highly problematic wastewaters not only because of high COD, BOD suspended solids, toxic compounds, but also because of color (dyes) which make them easily recognized and poses esthetic problems. When dyes are released in the environment, they form toxic compounds which harm the living creatures. Also the dyes color the water reservoirs which reduce the penetration of sunlight in turn leading to eutrophication. Presently there is no economical and efficient means or process to achieve the reduction of these two parameters. Now-a-days dye wastewater is treated by physical and chemical procedures which have many shortcomings. These physico-chemical methods available for the treatment of these synthetic dye-stuff, like flocculation, sorption, electrochemical and oxidative degradation. These physico-chemical methods available for the treatment of these synthetic dye-stuff, like flocculation, sorption, electrochemical and oxidative degradation which have limitations like cost effectiveness sludge or residue formation which is difficult to dispose off and also limited in their applications. Many studies have been focused on the microorganisms that are able to degrade the dyes, suggesting the bioremediation as an environment friendly and cost competitive alternative for dyed wastewater treatment. Decolorization of the synthetic
State Level Conference on ‘Microbology in 21st Century’
XXV
dye using different microorganisms appear to be effective, environment friendly, less expensive and with reproducible biotransformation data. Dye-stuffs are degraded by range of microorganisms in nature but studies are concentrated on bacterial and fungal decolorization of the dye. Release of azo dye in the environment is of great concern due to, colour, toxicity, mutagenicity and recalcitrant nature of the dye, considerable attention has been given in determining the ability of microorganism in decolorization and degradation of the azo dyes. In the present study, acclimatized microorganisms isolated from natural sources were used for the study of decolorization and degradation of the dyes viz. Acid Blue - 113, Acid Red - 1, Reactive Orange - 16, Direct Yellow - 50, Yellow - 4G, Orange - 2R, Methyl Red and Disperse Brown - 3REL. In all total 63 microorganisms were isolated, these isolates showed good decolorization pattern with average of 97.95% in nutrient broth condition. In Half Strength Nutrient Broth condition, the isolates showed 86.33% decolorization of the dyes. The cell free extract of the isolates showed 89.07% decolorization of the dyes. The percent COD reduction of the dyes was determined, the isolates showed upto 88.00% reduction in COD of the dyes. The degradation of the dyes was determined by GC-MS analysis technique. The microorganisms were identified by 16s rRNA analysis technique.
Key Words
Decolorization, Degradation, Aerobic, Bacteria, Azo Dyes, COD reduction
State Level Conference on ‘Microbology in 21st Century’
XXVI
Extended Abstract
Microbial Solubilization of Metals from Waste Printed Circuit Boards
A. M. Deshmukh
Department of Microbiology, B. A. M. U. Subcampus, Osmanabad 413501, Maharashtra
With the rapid development of technology, the problem of older electronic
equipments is increasing. The main reason behind such increase is low rate of recycling which, in turn, is due to the complex nature of such waste. Traditional ways for treating such waste are not economical and they contribute to pollution. The promising answer for the problem is bioleaching.
Studies were carried out to solubilize heavy metals from electronic waste. For this purpose, the consortium was obtained using a simple way, similar to that of ‘top down’ approach. Printed circuit boards (PCBs) were used as representative samples of e-waste. Various concentrations of PCB powder were used (1-5%) for bioleaching and their effects on metal solubilization, changes in pH and concentration of ferrous iron were accessed. Maximum level of metal solubilization was found to be 96.93% Cu, 93.33 % zinc for 10 g/litre of PCB powder and 10.26 % Ni for 30 g/litre of PCB powder. In case of lead, only 0.58% solubilization was achieved when 20 g/litre of PCB powder was used. The precipitate formed during bioleaching was analyzed by SEM EDAX which shows the presence of Tin (59.96%), Cu (23.97%), Pb (9.30%) and Fe(5.92%). The causes of copper precipitation were discussed.
Key Words
Microbial consortium, Printed circuit boards, Bioleaching
State Level Conference on ‘Microbology in 21st Century’
XXVII
Extended Abstract
Recent Trends in Microbial Control of Phytopathogens
S. B. Chincholkar, B. L. Chaudhari, P. D. Sarode and M. R. Rane
[email protected] School of Life Sciences, North Maharashtra University, Jalgaon 425001
Progress in molecular biology and analytical methods has helped in understanding of biological control of soil borne phytopathogens. There exists a great diversity among pathogenic fungi and bacteria. Therefore, control of such pathogenic micro-organisms is a great challenge. Plant Growth Promoting Rhizobacteria (PGPR) and Bio-control Agents (BCA) having multiple activities is a proper antidote against such phytopathogens. Earlier practices like – fighting of phytopathogens with soil fumigants and using agricultural practices like – crop rotation, tillage is either harmful or inadequate. The recent progress in technology focuses on knowledge on mechanism of competition of food, space, iron, parasitism, induction of systemic resistance and antibiosis. Recent approaches eg. identification of plant genes which improve plant interaction with disease suppressive bacteria, identification of genetic factors of biocontrol strain which alter/diminish biocontrol feature eg. phase variation, identification of factors produced by phytopathogens which alter biocontrol mechanisms eg. fusaric acid reduce anti-fungal metabolite production and also interfere quorum sensing/cell signaling, manipulation of soil mineral properties to optimize biological control would strengthen the efforts of agriculturists in time and space. Out of available novel biocontrol agents, our laboratory has concentrated on phenazines. Phenazines are heterocyclic, nitrogen containing, brightly colored secondary metabolites produced by a variety of bacteria. They exhibit anti-fungal as well as anti-bacterial activity. We concentrated on Pseudomonas aeruginosa 4365 which secretes phenazine-1-carboxylic acid (PCA) and pyocyanin. Standard protocol for laboratory scale production of PCA and pyocyanin was developed. Both compounds were purified to obtain crystals. This purified material was characterized with the help of UV-Visible Spectroscopy, IR, NMR and HPLC. Anti-fungal activity was studied in vitro. Pyocyanin, though a weak antifungal showed pH dependent inhibition of Scelrotium rolfsii. PCA is comparatively strong antifungal compound and inhibited Fusarium oxysporum, Sclerotium rolfsii and Colletotrichum falcatum at very low concentrations. Scaled up production of PCA at 12 L and 125 L was carried out. An attempt was made to evaluate comparative activity of PCA as a foliar fungicide through field trial on chilli which demonstrated that PCA has, if not better, comparable activity with various commercial chemical fungicides. Process details and other technical information will be discussed in conference.
Extended Abstract
Abstract of the Presentation
Dr. D.V. Gokhale
NCIM Resource Center, National Chemical Laboratory, Pune 411008, India
Microorganisms, their cells or their replicable parts are the tools for
biotechnology. These microbes are diverse and show numerous metabolic activities leading to products of immense industrial importance. The vast number of microorganisms has been isolated, identified conserved and utilized for benefit of mankind. However, 99% of the environmental microbes cannot be cultured under laboratory conditions. Thus the majority of the microbes thus remain hidden which need to be explored, identified and also conserved. These untapped microbial sources can be exploited using metagenomic approach to obtain novel microbial products. All these microbial strains need to be preserved in pure form without losing their genetic as well as phenotypic identity. Microbial Resource center (MRCs) act as custodians of such microbial diversity and play an important role in the storage and supply of authentic reference cultures for research and development. Microbial culture collections are established in many countries having variety of purposes. There are very small culture collections (200 microbial strains) and also large cultures collection possessing more than 50,000 microbial strains.
NCIM Resource Center is a pioneer microbial culture collection facility in India, which offers services to educational / research institutes and industries. NCIM is a unique resource dedicated to the isolation, collection, preservation and distribution of authentic cultures of industrially important microorganisms. NCIM holds about 3500 microbial strains of bacteria (1500), yeast (550), fungi (1300) and algae (15). The main objective of the facility is to supply authentic cultures to research institutes and industries. The catalogue of microbial strains can be accessed through http://www.ncl-india.org/ncim. Ours is small culture collection compared to ATCC and NCIMB. However, I will discuss some of the achievements emerging out of screening our own microbial sources.
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Isolation oDevelo
Depa
Trigomedicinal proin its root nodchemical fertorganoleptic qof a Rhizobicultivation cocharacterized 2% NaCl, antemperature ancomposition approach. Tdifferential efseedlings of cdifferent soilstreated with thpots seeded wcontrols, thoug
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
of Rhizobiumopment of Bi
Aasawa
artment of Micro
onella foenumgroperties which ardules. These proptilizers. Biofertiqualities of the lium strain isolaonditions and by analysis of
nd symbiotic pend pH sensitive,for laboratory
These isolates gffect on germinacultivars S1 and, SL1 and SL2. Ghe Rhizobium c
with Rhizobium,gh there was con
Arts, Science 11005
biology
OP1
m and Optimiiofertilizer fo
(Fenugr
ari Pawar and
obiology, The Ins
raecum (fenugrere supported by perties get compilizers are conseafy vegetable. ated from root application of growth rate, acierformance. Th, (optimum valu
scale growth grown on the oation of seeds a
d S2, in vitro inGermination rate
ultures, than con, seeds showed nsiderable differ
Mi
Published on 25t
11
ization of Gror Trigonella freek)
d Rajendra Ch
stitute of Science
eek) is known fosymbiotic nitrog
promised by imbsidered to mainThe present stunodules of feRhizobium bi
id production,40he Rhizobium ises of 25°C and 8was optimized
optimized mediuand plant growt
n sterile Petri-plawas higher in bontrols which weenhanced plant ence in growth p
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
owth Conditifoenumgraec
houre
e, Mumbai 40003
or its rich dietargen fixation by
balanced nitrogenntain the nutri
udy describes theenugreek, optimiofertilizer. Isol0oC tolerance, tosolates were fo8.0, respectivelyd using Plackeum were testedth promotion of ates as well as oth the seedlingsere untreated. In
growth than unpattern of test pla
Conference on
1st Century February 2011 tract Book
Page 1
ions for cum
32
ry proteins, Rhizobium
n supply as itional and e screening
mization of lates were olerance of und to be
y). Medium ett-Burman d for their f fenugreek
in vivo in s S1 and S2,
n pot assay, ninoculated ants.
Progressiv
Modeand CDepa
Isola
1Depar
Listehumans and apresence of Lclinical sampgenetical studmonocytogeneFurther seroty4b serotype aother ListeriaIsolates wereCiprofloxacinChlorampheni
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ation and ChEnviron
Abhay
rtment of Microb
eria spp. are ubianimals. 14 envListeria spp. Oules, 25 (18.79 dies confirmed es. CAMP and yping study of thand 6 were 1/2b
spp. No enviroe sensitive tow, Vancomycin icol, Penicillin, G
Listeria spp, A
Arts, Science 11005
biology
P
haracterizationmental and
Raorane¹, Ba
abhay.micro@biology New Arts
Ahmednag2ICAR, Ela, Old
iquitous in natu
vironmental and ut of 14 environ
%) were positithat 11(7.48%ALOA tests w
hese 11 pathogen serotype. Othernmental sampleward the antib
and shows Gentamycin.
Key woALOA test, CAM
Mi
Published on 25t
PP01
on of Listeria Clinical Sam
arbuddhe S.B
@gmail.com s Commerce andgar,
d Goa, Goa
ure. Out of 8 sp133 clinical sam
nmetal samples, ive for Listeria) were pathoge
were performed nic strain revealer species detectes contained path
biotics viz. Amintermediate r
ords MP test, Serotyp
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Species frommple
B.²
d Science Colleg
pp. 2 are pathomples were scre2 (14.28%) andspp. Biochem
enic strains of to confirm the ed that 5 isolatesed were L. ivanhogenic spp. of mpicillin, Doxyresistances tow
ping, AST.
Conference on
1st Century February 2011 tract Book
Page 2
m
ge,
ogenic to eened for d of 133
mical and Listeria isolates.
s were of novii and Listeria.
ycycline, ward the
Progressiv
Modeand CDepa
Antimicro
Aksha
1Deparm2Dept. o
historicaL. has endemicdermatopcardiac methanowater exconcentr50mg/mwere cheConcentcompoun
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
bial Activity Clematis Gou
ay Borkar, Ma
ment of Zoology, f Microbiology,
Vinca roseaally been used towound healing
c medicinal planpathy, blood disdisorders. Ethan
ol and water extrxtract of Catharation was studie
ml, 100mg/ml, 1ecked for Minimtration. Then thnds by Thin laye
Arts, Science 11005
biology
PP0of Vinca Ros
uriana agains
anoj Bhosale,Sonawan
AkshayBorkar1Annasaheb MagAnnasaheb Mag
a (Catharanthuso treat a wide as
activity in diant of western gseases, leprosy, nol extract and ract of Clematis aranthus roseused and methanol50mg/ml conce
mum Inhibitory he extracts werer Chromatograp
Mi
Published on 25t
02 sea (Catharanst Pathogenic
, Shirish Shahe H.V.2
@gmail.com gar College, Hadgar College, Had
s roseus L.) ansortment of dise
abetic patients. ghats and India,
wound healing,water extract ogouriana was st
s L. of 50mg/ml and water extraentration were sConcentration are evaluated tophy.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
nthus Roseusc Bacteria
h, Salokhe S.G
dapsar, Pune- 41dapsar, Pune-411
nd Clematis goueases. Catharant
Clematis gouriused in the tre
viral fever, heaf Catharanthus tudied. Ethanol eml, 100mg/ml, act of Clematis gstudied. All plaand Minimum Bo find out phy
Conference on
1st Century February 2011 tract Book
Page 3
s L.) and
G.1,
1028. 1028.
uriana has thus roseus iana is an eatment of adache and roseus L.,
extract and 150mg/ml
gouriana of ant extracts Bactericidal ytochemical
Progressiv
Modeand CDepa
Isolation
Alka Jag
Department o
Plantcan benefit pfertilizers andadvantageous phosphate solisolated from selected for thApplication opromoting actAcetic Acid pr
Phospha
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
and Screenin
gdale, Sharad
of Microbiology,
t growth promotplant growth. Gd there increas
for the devellubilization activfarm soil locatedhe further studiof Staphylococctivity. Staphylocoroduction.
ate solubilization
Arts, Science 11005
biology
PP0ng of Plant G
Rhizosp
ha Basetti, SmAnushka
, Modern college
ting bacteria (PGGiven the negasing cost, the lopment of susvity and 6 straind near Saswad. Tes and identifie
cus hominis cuoccus hominis a
Key Wo
n, Nitrogen fixati
Mi
Published on 25t
03 Growth Promophere
mita Bhuyan,Devale
e of Art, Science
GPB) are soil anative environmeuse of PGPB
stainable agricuns showing nitroThe isolate showed as Staphylocoulture with lignlso showed posi
ords
ion, plant growth
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
oting Bacteri
, Shilpa Muju
e and Commerce,
nd rhizosphere baental impact of
as natural feulture. 20 strainogen fixation actwing both the actoccus hominis bnite showed plaitive Siderophore
h promoting bac
Conference on
1st Century February 2011 tract Book
Page 4
ia from
mdar,
, Pune 05.
acteria that f chemical rtilizers is n showing tivity were tivities was by API kit. ant growth e and Indol
cteria.
Progressiv
Modeand CDepa
Isolationwaste
Ama
Departme
Polyhsynthetic plastdegraded comactivities of numerous baclimited concestudy is to isoβ- hydroxyalkthe growth parwater containicollected. It iDetection of PThe media opone of the vaeither one of tammonium Ph7.0 and 8.0), t24hr, 48hr anddesignated as Among these growth parampromising PHindependent e48hr, temperaof PHA by Wabove concent
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
n of Poly-β-He water and o
ar D. Jadhao,
ent of Microbiol
hydroxyalkanoattics because of t
mpletely to CO2
microorganismscteria as an int
entrations of nutlate the bacteria
kanoates and therameters such asing mostly sewais screened for bPHA production ptimization was arious carbon sothe Nitrogen souhosphate and yetemperature (ambd 72hr) were opt
Wi1 to Wi30 (four cultures W
meters for maximHA accumulatinexperiments 1g %ature 32oC and pWi2, Wi4 and Wtrations except p
Poly–β–hydrox
Arts, Science 11005
biology
PP0Hydroxyalkan
ptimization o
Hrishikesh D
viveknbobade@logy, Modern Co
Shivajinagar
tes are considertheir similar phyand water und
s. Poly-β-hydroxtracellular carbotrients with excfrom waste wat
eir characterizatis pH, temperaturage sample from bacteria produciwas done by Sucarried out usin
ources such as Gurces such as Amast extract. Othebient temperaturtimized for produ(W=Waste wate
Wi2, Wi4, Wi8, mum PHA prodng bacteria. A% glucose, 0.1
pH 7.0 were founWi8. Wi13 alsopH for maximum
Ke
xyalkanoate, PHA
Mi
Published on 25t
04 noate Producof its product
D. Dhamal, Viv
@gmail.com ollege of Arts, Scr, Pune 5.
ed good substituysical and chem
der natural envirxyalkanoates (Pon and energy cess carbon. Theter and that are con. The study are, time, nutrientSiddheshwar G
ing poly – β –udan black staininng modified E2 Glucose, Fructo
mmonium chlorider growth paramre, 28 oC, 30 oC, uction of PHA. r, i=isolate) werand Wi13 were
duction, since thmong the parag % ammoniumnd to be suitable
o shows maximum production of P
y Words
A, Optimization
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
cing Bacteriation paramet
vek N. Bobad
cience and Comm
utes for petroleuical properties aronment by the PHA) are synth
storage compoe objective of t
capable of produlso carried out tt concentrations
Ghat, Mulla riverhydroxyalkanoa
ng and Spectropmedium contain
ose, Maltose, Sude, Ammonium
meters such as pHand 32oC) and tThe bacterial isore found to prodselected for ev
hese are found tameters evaluat
m phosphate, time for maximum um production PHA is 8.0
of growth param
Conference on
1st Century February 2011 tract Book
Page 5
from ters
de
merce,
um derived and can get
enzymatic hesized by ound under the present
ucing poly -to optimize etc. Waste
r, Pune was ate (PHA).
photometry. ning either
ucrose, and sulfate and
H (5.0, 6.0, time (about olates were duce PHA. aluation of to be most ted in the
me of about production of PHA at
meters
Progressiv
Modeand CDepa
Bacterio
Department
The pprimarily chiland poor sanibenefit child quantity of wdisposal of thcontaminated bacteria at doof foods, at wdisposal of hudiarrhoea whithese agents, necessary to btransmission bwhereby childrinking wateimportant rolesupplies espec
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ological QuaHealth with
of Microbiology
primary justificadren, who suffeitation. In develhealth: an impr
water provided he human excret
with the infectises below those
which time the buman excreta shoich are eliminate
do not the tobreak the faecal by water and sadren’s health c
er support the stue in determinincially where the p
Arts, Science 11005
biology
OP0
lity of Waterh Special Ref
A. M. Ga
anilgarode@yy, Shri. Shivaji S
Buldha
ation for the decer because of inaloping countriesovement in the and used and pta. Diarrhoea caious agent of th
e necessary to inbacteria may incould play a role ied via the faces.ilet. Therefore, oral transmissio
anitation improvcan be improveudy. Excreta dis
ng children’s heprevalence of di
Mi
Published on 25t
03
r, Sanitation Iference to Di
arode
ymail.com Science and Arts ana
cade was to impadequate and cos, three basic tyquality drinking
provision of sanan occur followihe diarrhoea. Wanfect humans, mcubate and multin the control of Young childrenthe hygienic d
on of these pathvement is probaed. The bacterisposal appears toealth in developiarrhoea is high.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Interventionsarrhoea.
College, Chikhl
prove the health ontaminated watypes of the servg water, an incrnitation facilitieing the ingestioater containing p
may be used for ptiply in the foodf major infectioun, the primary edisposal of thei
hogens. Interruptably the major miological examio consistently pping areas, than
Conference on
1st Century February 2011 tract Book
Page 6
s and
i 443201,
of people, er supplies
vices could reasing the es for safe n of water pathogenic preparation d. Effective us agents of xcretors of ir faces is tion of this mechanism inations of lay a more
n do water
Progressiv
Modeand CDepa
CharaAntibacter
Dep Micr
production anthe cosmetic permanent, vadomestic envicause spoilagethe consumer the type of coload was founproducts and (2009). The coon the basis ofof isolates (20propyl parabeduring succesmineral salts mvarious concegrowth of foupresence of prestablished thThus, it was cosmetic produse of essentifor the growibecause of incthe antibacterithe contaminavariation in dHowever, limisolates. Concspectrum of ac
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
acterization orial Sensitivit
Ayushi.
apartment Of Mic
obial contaminand filling, and du
unit is openedariable and additironment and the of the productworldwide. The
ontaminants prend to be higher102cfu/g or ml fontaminants werf morphological
0) were studied inen as sole sourcsive enrichmentmedium by obseentrations (0.2-2ur isolates and ireservative as co
hat the propertynoted that the
ducts in respect al oils as functiing interest of ccreasing concernial activity of suants and 6 of tdegree of inhibi
me, eucalyptus anclusively, it wasction.
Arts, Science 11005
biology
Pof Contamin
ty against Syn
A. Chaurasiy
ayushi.chaurasiyacrobiology, Instit
ants in cosmetiuring the use by d until the contive microbial cohe consumer's bts and when patherefore, the studysent in various
r than the permifor eye based prre isolated, mainand biochemica
n presence of theces of carbon ats. The biodegraerving zone of h2%) of propyl it was observedompared to in it
y of utilization opreservatives wof their spectrumonal ingredientsconsumers in inn about potentia
uch 11 essential othem were founition; while 2 ond tea tree oil es established tha
Mi
Published on 25t
PP05 ants of Cosmnthetic and N
ya and R. B. Va
[email protected] tute Of Science, M
ic products havthe customer. F
nsumer finishesontamination of tbody. Contaminahogenic represeny was conductedintact and in usissible limit of roducts as per B
ntained on tryptonal characteristicse preservatives s
added separatelyadation of preserhydrolysis for eiparaben was st
d that the growts absence. The pof the paraben
were not successm of antibacteris in cosmetics isngredients fromlly harmful syntoils was screenend to be antibaof them showedexhibited inhibiat the natural es
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
metics and ThNatural Prese
aidya
Mumbai 400032
ve two origins From the momens the product, the cosmetic cauating microorgannt a serious hea
d to determine thse products. The103cfu/g or ml
Bureau of Indianne soya agar and. The biodegrada
such as methyl py to mineral salrvatives was conight isolates. Thtudied colorimeth was slightly aplasmid curing eis chromosoma
sful in preservatial activity. Nows gaining momen
m natural sourcethetic additives.
ed against the 20acterial in actiod no antibacteriaitory action agaissential oils wer
Conference on
1st Century February 2011 tract Book
Page 7
heir ervatives
2
i.e. during nt in which there is a
used by the nisms may
alth risk for he level and e microbial for topical
n Standards d identified able ability
paraben and ts medium nfirmed on he effect of etrically on affected in experiment
al encoded. tion of the w days, the ntum, both s and also Therefore, isolates of n showing al activity. inst all the re better in
Progressiv
Modeand CDepa
Study of Q
Totaltons and aquaquaculture inprawn cultureculture subsecwhich are bioconventional limited succesdrugs has ledantimicrobial bacteria (probinhibit their prconfirmed as their morpholculture showe.When luminebroth +Vibrioindicating quoshrimp’s intesfree filtrate ofbroth luminescould inhibit assay, by welisolates again14mm, 16mmgrowing VibriVibrio decreasfound to be nenvironment. probiont to ov
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Quorum Sensand A
Archa
l fish productionuaculture accounndustry is beset e industry. Disector. Major aquoluminescent baapproaches likess in the prevend to emergenceresistance to hu
biotics), which roliferation. BioVibrio by platin
logy. They wereed maximum bioescence induced o culture it worum sensing dstine & identifiedf a cocktail of thscence was delathe Vibrio isola
ll-diffusion methnst Vibrio cultur
m. To check the aio and the Bacilsed after 6 hrs &non-hemolytic wThe Bacillus iso
vercome Vibrio in
Arts, Science 11005
biology
P
ing and QuoApplication i
ana J. Marry a
Sophia Colleg
n in the world mnts for 47%. Hby bacterial and
ease has becomeuaculture pathogacteria commone use of disinfetion or cure of ae of more viruuman pathogensare environmen
oluminescent bacng on Thiosulphe identified as Voluminescence in
cell free supernwas observed thdue to autoindud tentatively as b
hese 5 isolates wayed indicating ate when testedhod, using indivre showed zoneantagonistic potellus culture toge
& there was no grwhen grown on b
olates can thus nfection in shrim
Mi
Published on 25t
PP06
rum Quenchin Aquacultu
and Jyoti Man
ge, Mumbai
market is estimateHowever, in mod viral diseases, e a limiting fac
gens are Vibrio nly found in aquectants and antimaquatic diseases
ulent pathogens s. In this study, ntally safe to dicteria were isolahate citrate bile Vibrio fischeri bn 10 hours whennatant broth was hat luminescenc
uction,. Five isobelonging to Ba
was added to the quorum quench
d by cross-streakvidual cell free ees of inhibition ential, co-cultureether & it was forowth at 48 hrs. blood agar thus be used individ
mp aquaculture.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
hing in Vibrioure
ntri
ed to be above 1st of the Asianespecially the s
ctor in prawn aharveyi & Vibruatic environmemicrobial drugs
s. The use of anand eventual twe tried using,
isplace the pathated from squid .
sucrose agar anby biochemical n grown in sea wadded to a fresh
ce occurred witolates were obtaacillus species. WVibrio culture inhing. All these king method. Aextract of the fivof 12mm, 15m method was per
found that viableThe Bacillus isocan be safely u
dually or as a co
Conference on
1st Century February 2011 tract Book
Page 8
fischeri
110 million n countries shrimp and and shrimp rio fischeri ent. So far s have had ntimicrobial transfer of , beneficial hogens and .They were
nd studying tests .This
water broth h sea water thin 6 hrs ained from When a cell n sea water
5 isolates Antagonism ve Bacillus
mm, 13mm, rformed by e counts of olates were used in the ocktail as a
Progressiv
Modeand CDepa
Bi
Abhijit Mo
Micro Envirthe explosivesThe nitroexplshowing symbiodegradation1,3,5-triaminobenzene ring attached, alterby HEMRL, Pstarting matetrichlorotrinitramination stephigh nitrate, aTATB is knownitro group dioxygenase ethe present stufrom TATB pOut of these, TATB as solmedium at therange of 20-3Mingioli’s syn
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
odegradation
ore, Sayali Ma
bial Sciences Di
ronmental contas industry. Howlosive as well amptoms as kidn of recently d
o-2,4,6-trinitrobestructure with t
rnately to the rinPune is a two-serial. In firstriobenzene (TCp. Wastewaters gammoniacal nitrown as a recalciton the aromati
enzymes involveudy, total 18 baproduction site u
only two bactele source of nite concentration o30% and nitratenthetic medium s
Arts, Science 11005
biology
PP0n of Triamino
ahajani, Seem
ivision, Agharka
amination of nitrever, global proas their environdney trouble, developed insenenzene (TATB)three nitro groung. The current tage process usit nitration steTNB) which thgenerated duringogen and chloridtrant compound ic ring, an arr
ed in the microbacterial isolates wusing enrichmenerial isolates antrogen when incof 100 mg/l. Thee in the range supplemented w
Mi
Published on 25t
07 otrinitrobenz
ma Sarnaik an
ar Research Insti
rocompounds is oduction and expnmentally transfjaundice etc.
nsitive high ene). It is an aroups (NO2) and tprocess for the ing 1, 3, 5-trichep, trichlorobehen is further g their productiode content causibecause of the
rangement that ial metabolism owere obtained frnt, adaptation annd one isolate ocorporated in De isolates showeof 20-35% wh
with 0.05% pepto
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
zene (TATB)
nd Pradnya K
itute, Pune 411 0
associated princplosive use is unformed products
Present studieergy nitroexplosomatic explosivthree amino (NHproduction of T
hlorobenzene (TCnzene is conconverted into
on are highly aciing environmentsymmetric localimits attack
of aromatic comrom soil samplend soil baiting tof actinomycete Davis Mingioli’sed removal of TAhen incorporatedne.
Conference on
1st Century February 2011 tract Book
Page 9
Kanekar
004
cipally with navoidable. s are toxic es include sive (IHE) ve, having H2) groups
TATB used CB) as the
nverted to TATB in
idic having tal hazards. ation of the
of classic mpounds. In es collected techniques.
could use s synthetic ATB in the d in Davis
Progressiv
Modeand CDepa
Produ
Departme
Starcfermentation, various samplpresent investAgricultural Cmost promisinfurther study. The optimum observed at 1a8 for optimum
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ction of Am
Bedare
ent of Microbiol
ch degrading batextile and pap
les has manifoldtigation bacteria
College, Pune. Sng amylase produ
Growth patterntemperature for
and 2% starch com growth. Maxim
A
Arts, Science 11005
biology
PP0
mylase by BSamp
Monika and M
logy, Modern CoShivajinagar, P
acteria are mosper. Thus isolatd importance foal strains were i-5 & Si-6 bacteucing bacteria ams as well as optir these strains woncentration respmum enzyme acti
Key Wo
Amylase, soil, sta
Mi
Published on 25t
08
Bacteria Isople
Madhuri Amru
ollege of Arts, ScPune 411 005
t important forting and manipur various biotecisolated from soerial isolate frommong 15 isolatesimum growth cowas 37oC, wherepectively. The pHivity was observ
ords arch degrading
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
olated from
utkar
cience and Comm
r industries suchulating pure cu
chnology industroil sample colle
m soil were founs & these were sonditions were deas maximum gH range was fouved at this condit
Conference on
1st Century February 2011 tract Book
Page 10
Soil
merce,
h as food, ulture from ries. In the ected from
nd to be the selected for determined. growth was und to be 7-tion.
Progressiv
Modeand CDepa
Antiplas
The mproblem and mgenetic determbacteria. The and aqueous clinical isolatewere isolatedAzadirachtaincuring activitygrowth in the derivatives waaqueous and MRSA. Such making them may be a sodevelopment a
Antibacteria
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
mid Activity
A.D Bho
Department of
methicillin resistmajor challenge
minants that conpresent investigextracts of leaves. Active fracti
d from the orgndica, Annonasqy of organic ext presence of antas further confirmorganic extractplasmid loss r
sensitive to low ource to develoand spread of pla
al Activity, Antib
Arts, Science 11005
biology
OP0
of Herbal ExStaphylococc
lay *, Katkad
adbholay@gf Microbiology, K
tance Staphylocoe to the global dnfer resistance togation was underves of different ions demonstratganic and aquequamosa, Ocimtracts wasdetermtibiotics. The phmed by agarose s of leaves of eversed the mu concentrations
op antiplasmid asmid borne mul
Key Wobiotic Resistance
Plasmid C
Mi
Published on 25t
08
xtracts on Mcus aureus
de A.N,Paleka
gmail.com K. T. H. M. Colle
occus aureus (Mdrug discovery po antibiotics arertaken to investiplants to cure
ing antibacterialeous extracts omumsanctum, Pmined by evaluathysical loss of pgel electrophoretested plants cu
ultiple antibioticof conventionalagent of natu
ltiple antibiotic r
ords: e, Antiplasmid ACuring
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Methicillin Res
ar K.S.
ege, Nashik
MRSA) is a seriprogrammers. M
e located on R-pigate the ability R- plasmids fro
l andant plasmidof shade dried Pongamiapinnatating the ability oplasmid DNA inesis.The active fured the R-plas
resistance in cl antibiotics. Theral origin to cresistance.
Activity, Herbal
Conference on
1st Century February 2011 tract Book
Page 11
sistant
ious health Most of the plasmids in
of organic om certain d activities
leaves of a. Plasmid of bacterial n the cured fractions of mids from uredstrains
ese extracts control the
Extracts,
Progressiv
Modeand CDepa
Charact
1D 2Hea
Cellu
compounds in
promote solu
application of
vegetables to
removing bitte
study is to iso
water. These
parameters of
Cel
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
erization of Tβ-Gluc
Bhalkikar S
Department of Mad, Department o
ulose is most abu
n terrestrial bio
ubilization of c
f β glucosidase i
aglycone moie
er compounds fr
olate bacteria fro
isolate would
f these crude filtr
llulose, β-Gluco
Arts, Science 11005
biology
PP0
Thermo-Toleosidase from
S. B., Diwan V
Microbiology, Daof Microbiology
undant on earth c
omass. Endo and
crystalline cellu
include converti
eties, detoxificat
rom citrus fruit j
om raw material
be screened for
rate would be det
Key Wosidase , Acid /A
Mi
Published on 25t
09
erant and Acim Bacterial Iso
V. A.1, Ranjeka
ayanand Science , Dayanand Scie
constituting abou
d exo glucanas
ulose into solu
ing phytoestroge
tion of cassava,
uices or unripe
l that is rice stra
r β glucosidase
tected.
ords lkali Tolerant, T
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
id /Alkali Toolate
ar M. K..2
College, Latur ence College, La
ut half of the car
es act synergist
uble sugars. B
en glucosides in
, aroma enhanc
olives. The purp
aw and unai ther
e production and
Thermo-Tolerant
Conference on
1st Century February 2011 tract Book
Page 12
lerant
atur
rbonaceous
tically and
iotechnical
n fruits and
ement and
pose of this
rmal spring
d different
t
Progressiv
Modeand CDepa
Detoxica
Bhola
Azo as leather, texgroup of dyeshas been estimto the fibers astructural propmicroorganismfound to catamicrobial speselected for fufour species c(Reactive red-(Malachite grphysiological yellow- 145 woptimization decolorizationcultures showpure cultures tand detoxicatiseen within lesproduces varistudies. Detoxconsortia provfurther charac
Detoxic
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ation of Azo D
ay A. D., Maid
Department of
dyes are used wtile, plastics, pap
s used in industrmated that 10% and is therefore perties that are
ms such as aeroalyze dye decoloecies were isolaturther study out can decolorize -195, Reactive breen) and ‘simuand metabolic a
was selected as mof physicochem
n within three dws 80% of decolo
the bacterial andion. Promising rss period of timeious ‘aromatic xication studies ved to be significterized by activa
cation, Azo Dyes
Arts, Science 11005
biology
PP1
Dyes by MicrMicrobial C
d Sneha A., Bo
of Microbiology,
widely in the manper, hair care prory representing mof the dye stuff released in to thnot easily degra
obic and anaeroborization and dyted. After screeof them two ar
and detoxify ‘Ablack-5, Reactiv
ulated effluent’ (aspects of decolomodel azo dye umical paramete
days under statioorization withind actinomycetal results were obtae than that of puramines’ which were carried oucantly efficient tated sludge proce
Key wos, Reactive Yello
Mi
Published on 25t
10
robial Pure CConsortia
orse Priti k., R
K.T.H.M Colleg
nufacture of varoducts and cosmmore than half of during this dyinhe sewage or enadable under nabic bacteria andye detoxication.ening the most re bacteria and tAcid dyes’ (Metve yellow-145), (Mixture of 10
orization and detunder static and
ers bacterial puonary conditions
n 5 days under sconsortia are alsained about 95%re cultures. The b
were confirmeut in vivo by seethan the individuess for heterogen
ords ow, Consortia, F
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Cultures as w
Rakte Snehal
ge, Nashik.
rious consumer gmetics. Azo dyes of the annual prong processes dos
nvironment. Azoatural conditionsd actinomycetes
From textile efefficient four ctwo are actinomthyl red), ‘Reac‘Tryphenyl medyes). In orde
toxication procesd shaking conditure cultures shs and Actinomyshaking conditioso tested for dec
% to 96% of decbiodegradation oed by ‘TLC’ aed germination. ual isolates, whicneous dye efflue
FTIR, Decoloriza
Conference on
1st Century February 2011 tract Book
Page 13
well as
B.
goods such are largest
oduction. It se not bind dyes have
s. Different have been
ffluents 25 ultures are
mycetes. All ctive dyes’ ethane dye’ er to study ss Reactive tions. After hows 90% ycetes pure ns. Instead
colorization colorization of azo dyes
and ‘FTIR’ The mixed ch could be ents.
ation
Progressiv
Modeand CDepa
Probiotic PAnimals a
1Department o2Departm
Probi
resistance aga
120 milk sam
isolates were
recognized as
excellent pro
potential with
plus, P-Biotic
plantarum (M
exhibited exc
and standard p
from milk of
infections and
Probiotics, L
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Properties ofand Commer
En
Bhutad
of Microbiologyment of Microbio
iotic is the group
ainst intestinal p
mples (40 each
identified as Lac
s prominent pr
obiotics. These
h commercial pro
s kid, Gastroline
MTCC 2621) an
ellent probiotic
probiotic bacteri
f domestic anim
d contributes hea
Lactobacillus, A
Arts, Science 11005
biology
PP1
f Lactobacillucial Availabl
nteric Bacteri
da S. A.1 and
sabhutada13@, New Arts, Comlogy, S.G.B. Am
p of microbes th
pathogens and in
from buffalo, c
ctic Acid Bacter
robiotics, among
excellent prob
obiotic preparati
e, Pre-Pro kid an
nd L. rhamnos
characteristics
ial strains. Study
mals can be he
alth benefits to co
Key Wo
Antibacterial Acti
Preparat
Mi
Published on 25t
11
us Isolates frole Probiotic Pial Pathogens
Tambekar D.
@gmail.com mmerce and Sciemravati Universit
hat may be helpf
n the prevention
cow and goat)
ria (LAB). Out o
g them 3 isola
biotics were co
ons such as Spo
nd standard prob
us (MTCC 104
than commercia
y suggested that
lp to prevent o
onsumers.
ords
ivity, Bacterioci
tions
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
om Milk of DPreparations s
. H.2
ence College, Ahty, Amravati, 44
ful directly for e
n of diseases. A
were analyzed
of these 11 isola
ates were ident
ompared their p
orlac powder, La
biotic bacterial s
48). The isolat
al probiotic prep
use of probiotic
or control the i
in, Commercial P
Conference on
1st Century February 2011 tract Book
Page 14
Domestic against
hmednagar, 44602,
enhanced
A total of
and 110
ates were
tified as
probiotic
actoBacil
strains L.
ed LAB
parations
c bacteria
intestinal
Probiotic
Progressiv
Modeand CDepa
Isola
Bhadale
Departmen
Lipas
most widely u
current and p
industrial enz
pharmaceutica
(triacylglycero
or together wi
extracellular,
oil interface.E
In general, the
the use of wa
lipase produce
is an inexpe
tributyrin wer
and Rhodam
presence of u
wavelength o
precipitation m
enzyme was s
fungi and acti
various substr
producer by a
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ation, Purifica
S. V., Bhalera
b
nt of Microbiolog
ses are used in t
used as biocataly
potential applic
zyme. These ar
al preparations,
ol acylhydrolase
ith esterases (car
hydrolytic enzym
Even though Lipa
e use of water so
ater in-solublesub
ers is done by us
ensive substitute
re added to min
mine B agar te
uranyl ions, yie
of 350nm. Enz
method. Molecu
studied. Most co
inomycetes. The
rate selectivity i
actinomycetes f
Arts, Science 11005
biology
PP1
ation and Ap
ao M. N., Pati
bhadalesujeet123
gy, Annasaheb M
the food, dairy, d
yst in the field
ation lipases ar
re currently us
flavour compo
e; EC 3.1.1.3) ar
rboxylic-ester hy
me because they
ases and esterase
oluble substrates
bstrates is consi
sing tributyrin(9)
e for trioleoylg
nimal medium an
est was done fo
elding orange fl
zyme purificatio
ular weight was d
ommercially ava
e search for new
s still going on.
from hot water.
Mi
Published on 25t
12
pplication of L
il N. N., Ghule
Magar College, H
detergent, cosme
of organic chem
re considered t
ed in the prod
ounds and other
re produced by a
ydrolases; EC 3
y act on substrat
es differ in their
is considered di
idered diagnostic
) or Tween 80(1
glycerol. Then
nd activity were
or confirmation.
fluorencent comp
on was done
determined by S
ailable lipases ar
w lipases with g
Hence this wor
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Lipase Enzym
e P. V. and Ku
Hadapsar, Pune
etic, tanning ind
mistry. In the vie
to be promising
duction of fine
r food additativ
actinomycetes e
.1.1.1).Lipases a
tes providing an
r ability to act at
iagnostic for est
c for Lipases Sc
2) as a substrate
various concen
e studied . Tribu
Rhodamine B
mplexes with an
by Ammonium
SDS-PAGE. App
re synthesized b
greater thermost
rk is being done
Conference on
1st Century February 2011 tract Book
Page 15
me
ulkarni
411028
dustries and
ew of their
g class of
chemical,
ves. Lipase
either alone
are unusual
n water and
Interfaces.
erases, and
creening of
e .Olive oil
ntration of
utyrin agar
is used in
excitation
m sulphate
plication of
by bacteria,
tability and
e for lipase
Progressiv
Modeand CDepa
Ant
Post Gradua
The
meaning 'life'.
spp. Produce m
-methyllanth
antibiotics, an
peptide antibi
against Gram
methicillin-res
Staphylococcu
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
tibiotic Produ
Chavan
ate Department o
word antibiotic
. There are man
many type of an
hionine. It is cla
nd is produced b
otic isolate from
m-positive bac
sistant strains.Th
us spp.
Arts, Science 11005
biology
PP1
uction from B
Staphylococc
K. J., Patil S.
of Microbiology,
comes from th
ny Bacteria can
tibiotics. Mersac
assified as a mem
by a species of B
m fermentations
teria, particula
his Meracidin an
Key woMersacidin, Ba
Mi
Published on 25t
13
Bacillus spp. A
cus aureus
D., Ranjekar
, Dayanand Scie
he Greek anti m
produce antibio
cidin is a new pe
mber of the pro
Bacillus. It is pe
of a Bacillus sp
arly Staphyloco
ntibiotic is activ
ords acillus spp.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Active agains
r M. K.
ence College Lat
meaning 'against
otic. Among thes
eptide antibiotic
oposed lantibioti
eptide antibiotic
pecies. It is ma
occus aureus,
ve against many
Conference on
1st Century February 2011 tract Book
Page 16
st
tur, India.
t' and bios
se Bacillus
containing
ic group of
. This new
ainly active
including
y strains of
Progressiv
Modeand CDepa
Antimicr
Bholay A.
Depart
The marine tidal grasses whichthe most proddirectly or indMangroves arerosion and cylike tannin exuses and severunexplored. Tof India are Xylocarpus ggymnorrhiza, and mangrovantifungal comflavonoids, althe presence fungal strainsPseudomonas were resistanAmphicillin +different conc,become sensiare more potenpathogens is cof mangrove wresistance proloss of plasmsynergestic eantibiotics are
Mangroves, M
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
robial Activity
D., Kurup Sun
tment of Microb
term mangroveforests compris
h are associated iductive and diverdirectly dependere highly productyclonic conditioxtraction, paper ral other by-pro
The most domina:- Rhizophora
granatum, X.moB.parviflora, S
ve associates mpounds. They akaloids and tannof new antibac
s. The MDR p , Staphyllococcu
nt to drugs lik+Sulbactam,Spacentration in twoitive to the abovnt as compared t
carried out in Muwas obtained by
operties are assomid were confireffect of mixede under experime
Multidrug-Resist
Arts, Science 11005
biology
PP1
y of Mangrov(MDR) Pa
nila B., Vidha
adbholay@g
iology ,K.T.H.M
e is also used sing of trees, sin strands or grorse in the world ent on mangrovetive and econom
ons. This uniqueand pulp, firew
ducts.The antimant mangrove spmucronata, R.alluscensis, Avic
Salvadora persiccontain biologalso provide richnins. Therefore
cterial compoundpathogens like us aureus , Staphke Amicasin,
arfloxacin were o different solvene mentioned druto that prepared uller –Hington ay Sequential Soxciated with R –rmed by Agard extracts and entation.
Key Wotance, Unexplor
Mi
Published on 25t
14
ves against Mathogens
ate Pushpa S.,
gmail.com
M. College,Nashi
to designate Fshrubs,palms, epoves.These wetlaand more than
e and other coasmical which alsoe tree resource iswood, timber, ch
microbial activitypecies found aloapiculata, Sonnecennia officinalica, Sesuvium po
gically active ah source of steroit is worth to scds to combat th Streptococcushyllococcus xyluNetilmycin , Gtreated with t
nts (EA, MeOHugs.Extracts prepin Methanol (M
agar by Agar Gelxhlet Extraction
–plasmids.The phrose gel electro
the potentiatio
ords ed, Synergestic E
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Multidrug-Re
, Rahatekar R
ik.,Pune Univers
Facultative haloppiphytes,ground and ecosystems 80% of marine c
stal ecosystems w protect the shors usedfor variouharcoal ,fodder,y of mangroves
ong the east and eratia alba, S. is, A. marina, ortulacastrum. Mantiviral,antibacoids , triterpenescreen mangrovehe pathogenic bs , E.coli , Kleulose , XanthomoGentamycin, Cthe mangrove e
H) in Muller-Hinpared in Ethyl ac
MeOH). Sensitivitl Diffusion Methmethod. Most o
hysical partial oophoresis technon activity with
Effect, Potentiat
Conference on
1st Century February 2011 tract Book
Page 17
esistant
Rohini R.
sity.
phytic and ferns and
are among catches are worldwide. reline from
us purposes medicinal are largely west coast caseolafis, Bruguiera
Mangroves cterial and s, saponins, e plants for bacterial & ebshiella , onas which
Cefotaxime, extracts at
ngton Broth cetate (EA) ty of MDR hod. Extact of the drug
or complete nique. The h standard
tion Effect
Progressiv
Modeand CDepa
Biosorptio
Chro
bioaccumulati
microorganism
research empl
effects of pH
contact time w
Chromium w
treatment of b
The optimum
and 150 rpm r
the metal as w
initial contact
and Langmuir
10mM NaOH
carboxyl grou
from potentio
the fungal my
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
on of hexavadead fun
Dee
Departmen
15 Ma
omium presents
ion and bioma
ms for removal
loyed dead biom
, temperature, s
were examined.
with a decrease
biomass using 1
temperature and
respectively. Th
well as biomass.
exhibiting 1st o
r Isotherms. De
H at S/L of 2 f
ups of the fungal
metry and FT-IR
celium.
Arts, Science 11005
biology
PP1
lent chromiugal biomass o
ep.V.Barot & V
barot_deep@y
nt of Microbiolog
adam Cama Roa
s threat to th
agnifications he
of Chromium
mass of P. chry
haking speed, m
pH was the mo
in pH. Bios
1M phosphoric a
d shaking speed
he removal of Ch
The biosorption
order kinetics. S
esorption and re
for five biosorp
l cell wall contrib
R analysis. SEM
Mi
Published on 25t
15
um from aqueof P. chrysog
Varsha.Vaidy
yahoo.co.in
gy, Institute Of S
ad Mumbai-4000
he environmen
ence necessitati
by biosorption
ysogenum in bat
metal concentrat
st critical factor
sorption was en
acid coupled wi
for the biosorpt
hromium was af
n reached equilib
Sorption data we
e-usability studie
ption -desorptio
buted to the sorp
M indicated accu
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
eous solutiongenum
ya
Science,
032
nt due to its
ng its remova
is gaining atten
tch experiments
tion, mass of bi
r increasing the r
nhanced from 1
ith autoclaving t
tion were found
ffected by conce
brium after 165
ere described by
es indicated use
n cycles. The
ption process as
umulation of Ch
Conference on
1st Century February 2011 tract Book
Page 18
ns using
s toxicity,
al. Use of
ntion. This
where the
iomass and
removal of
13.19% by
to 22.93%.
to be 25°C
entration of
min of the
freundlich
efulness of
amine and
confirmed
hromium in
Progressiv
Modeand CDepa
Study o
De S
2Institute o
Campfor both humhuman CampCampylobacteproduction. Ccommonly assthan those causystemic casesas they are sechoice to treatantibiotics inCampylobacteantibiotics belaminoglycosidciprofloxacin,slaughter hou(MIC) as wellthe agar dilutaccording to Institute). Brevalues for azitis also necessplasmid or chaims at stu
Agar D
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
of Antibiotic RIsolat
ouza N. A.1, K
1Departmeof Bioinformatics
pylobacter are tan and animal dpylobacter infecer mainly as a cCampylobacter jsociated with baused by Salmons, most cases of lf-limiting. Mact such infections
ncluding trimether isolates werelonging to the cdes were selecte gentamicin and
uses around Punl as the minimumtion and broth m
breakpoints speakpoints for resthromycin and esary to determinhromosomally mudying the pre
Dilution, Antibiot
Arts, Science 11005
biology
PP1
Resistance Pated from Pun
Kamble A.2, P
bpkap@unipent of Microbiolos and Biotechno
thermophilic Gradisease. Poultryctions. Antimionsequence of tjejuni and Camacterial diarrhoenella and E.colif campylobactericrolides and fluos. Campylobactehoprim, vancom studied for theclasses macrolid
ed for the study. d tetracycline. Thne, Maharashtram bactericidal comicrodilution mepecified by CLsistance to ciproferythromycin arene whether the
mediated by studevalence of an
Ketic Resistance, B
Mi
Published on 25t
16
atterns in Cane, Maharash
arkar S. D.1,
pune.ac.in ogy, University ology, University
am negative enty is one of the mcrobial resistan
the unchecked umpylobacter coliea. C. jejuni acc
combined. Excosis do not requ
oroquinolones arr shows intrinsic
mycin and cefoeir antimicrobialdes, fluoroquino
They included he cultures used a. The minimumoncentration (Methods. The MICLSI (Clinical afloxacin are estae ≥8 µg/ml and resistance to th
dies involving pntibiotic resista
y Words Broth Microdilut
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ampylobacterhtra
Kapadnis B. P
of Pune of Pune, Pune 4
eric pathogens rmost important nce has emerguse of antibioticsi have been focounts for morecept for certain uire antimicrobiare regarded as thc resistance to a operazone. In tl susceptibility polones, cephaloserythromycin, cwere isolated fro
m inhibitory conBC) was determC values were d
and Laboratory ablished at ≥4 µ≥32 µg/ml resp
he antimicrobialplasmid curing. ance in Camp
tion, Campyloba
Conference on
1st Century February 2011 tract Book
Page 19
r spp.
P.1
411 007
responsible sources of ed among
s in poultry ound to be e infections severe and
al treatment he drugs of number of
this study, pattern. 25 porins and
ceftriaxone, om poultry ncentration
mined using determined
Standards g/ml while
pectively. It l agents is This work
pylobacter.
acter
Progressiv
Modeand CDepa
AntimicrCommonly
Gauri D
1Post gr
2Research Ce
Elimibacteria like Sthe field of mantibiotics speacquired infecsafe drugs toeffective antibpharmacologicsulphanylamidbiologically acto increase thactive groups.SDZ. Our chiagainst some pathological sidentified as Edevelop resistmost commonpump may beassayed by wboth clinical iexhibited a gtrimoxazole w
Multidrug R
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
obial Activityy Occurring M
evasthale1, A
raduate departmA
enter in Chemist
ination of the mStaphylococcus, medicine. These ecially sulphonactions. It is thus fight these nobacterial agentscal activities. de and structurctive Gallic acid
heir antimicrobia. The compoundief aim was to c
of the most csamples. . The E.coli, Staphylotance to sulphonn and basic mece present in the
well diffusion meisolates as well good antibacteri
was used as a stan
Resistant Pathoge
Arts, Science 11005
biology
PP1
y of New SulpMultidrug Re
Alok Vyas2, Di
dgauri1972@yent of Microbiol
Azam Campus, Ctry, Abeda Inamd
Pune-
most widespreadE.coli and Pseustrains have ac
amides. They ares the need of theosocomial pathos for many year
Seven new cally characteriz
d moiety were al spectrum. Th
ds were named acheck the activitommonly occurorganisms wer
ococcus and Psenamides rapidly achanisms of mu
resistant cells. ethod for antimas standard cul
ial activity. MIndard.
Keywoens, Antibacteria
Schiff B
Mi
Published on 25t
17
phanylamideesistant Urin
vya Nair1 and
yahoo.co.in logy, Abeda Inam
Camp, Pune- 1 dar Senior Colle- 1
d multidrug resisdomonas has be
cquired resistance also responsible hour to develogens. Sulphonamrs and are wellcompounds werzed. The sulfonincorporated int
he side chains was STZ, IND, THty of these newrring multidrugre isolated fromeudomonas. It seand efflux pump
ultidrug resistancThe newly syn
microbial activityltures. Among thIC and MBC w
rds: al Activity, STZ
Bases
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
e Analogues anary Tract Pa
d Jhilmil Gho
mdar Senior Col
ege, Azam Camp
stant strains of ecome a major chce to most of thle for many of thop new more effmides have beel known for there synthesiamide- Schiff bto these moleculwere substitutedHB, DHB, CHRly synthesized cresistant patho
m urine sample eems that these ps are considerece. One or mor
nthesized compoy against these he tested compowas also perfo
, Sulphanylamid
Conference on
1st Century February 2011 tract Book
Page 20
against athogens
oshal1
llege,
us, Camp,
pathogenic hallenge in he existing he hospital fective and en used as eir diverse ized from bases with les in order d with new , SNL, and compounds ogens from
and were organisms
d to be the e than one
ounds were pathogens
ounds STZ rmed. Co-
de, UTI ,
Progressiv
Modeand CDepa
Isolation
Bacteria fr
Gauri Paw
Departm
Biolu
specific wavel
Some marine
showing symb
bioluminescen
luminescent d
parameters su
Maximum lum
produced by d
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
n, Identificati
rom Fish Sam
war, Deepali M
ment of Microbiol
uminescence is
lengths are emitt
fishes have evo
biotic associatio
nt bacteria both
diplococci were
uch as temperatu
minescence was
diplococci are ca
Bio
Arts, Science 11005
biology
PP1ion and Char
mple and Its U
Mane, Vyanka
and Smita B
logy, Modern co
Shivajinagar Pu
the light emis
ted due to conve
lved the ability,
on. These anima
a safe place to
e isolated from
ure and pH were
s observed at 2
atalase, oxidase a
Key wo
oluminescence,
Mi
Published on 25t
18 racterization
Use as Water
atesh M. Sana
Bhuyan*
ollege of Arts, Sc
une 411 005
ssion phenomen
ersion of chemica
to harness these
als have special
live and a sour
marine squid.
e observed on pr
260 C and at p
and esterase.
ords
Squid, Esterase
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
of Biolumine
r Pollution In
am, Shilpa Mu
cience and Comm
non in which p
al energy into lig
e light-producing
l light organs th
rce of food. Gra
Effects of env
roduction of lum
ph 8. The enzy
Conference on
1st Century February 2011 tract Book
Page 21
escent
ndicator
ujumdar
merce,
photons of
ght energy.
g microbes
hat provide
am Positive
vironmental
minescence.
ymes were
Progressiv
Modeand CDepa
Assessmen
1Department o
Inforgermplasm is The genetic diinvestigated pcultivars usinprimers were similarity betwutilized to con(UPGMA). Tsegregation amgene pool orig
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
nt of Genetic
Hris
of Biochemistry,
rmation about vital for develo
iversity/relatednpreviously. We
ng randomly amsurveyed, out
ween genotypes nstruct a dendog
The genotypes wmong varieties fogin.
RAPD,
Arts, Science 11005
biology
PP1
c Diversity amMark
shikesh Joshi
New Arts Comm2ICAR, Ela, Old
the extent of oping coherent s
ness among manghave assessed
mplified polymorof which 15 wwas in the range
gram using the uwere grouped infound may be du
Key wo
Germplasm, UP
Mi
Published on 25t
19
mong Mango kers
¹, A. R. Desai
merce and Sciencd Goa, Goa.
genetic diversstrategies for fugo cultivars deve
the genetic divrphic DNA (RA
were selected foe of 0.10 to 0.66%unweighted pair nto two main clue to less geograp
ords
PGMA, Dendogr
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Varieties by
i²
ce College, Ahm
sity/relatedness uture gains in preloped in Goa haversity among
APD). 25 randoor further studie%. These coefficgroup of arithmlusters. But no phic distance an
ram.
Conference on
1st Century February 2011 tract Book
Page 22
y RAPD
mednagar
in mango roductivity. as not been 16 mango
om ten-mer es. Genetic cients were
metic means significant
nd common
Progressiv
Modeand CDepa
Develop
1. D2. Head
Biostgrowth. Biostitexture,improveffect of pH fertility whimicroorganismbacteria livingmicroorganismuse as biostim
Soil Fertility
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ment of MicrSaline So
Indulkar V.
Department of Md, Department of
timulants is a claimulants enhancve plant ability and soil colloid
ich are nitroms, siderophoreg around root ms as well as sa
mulant to enhance
y, Saline Soil, Sa
Arts, Science 11005
biology
PP2
robial Biostimoils for Susta
.S.* Siddiqui A
Microbiology, Dayf Microbiology,
ass of product wces shoot and roo
to recover fromdal imbalance. Vgen fixing mproducing micr
system. Presentalt tolerant Azotoe fertility of salin
Key Woalt Tolerant Mic
Biostimu
Mi
Published on 25t
20
mulants to Enainable Agric
A.G.1, Ranjek
ayanand Science Dayanand Scien
which enhances sot development,m disease and inVarious microormicroorganisms,roorganisms, fut work is aimedobacter species ne soil.
ords croorganisms, Saulants
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
nhance Fertiulture
kar M.K.2
College, Latur. nce College, Latu
oil fertility as wimprove soil str
nsect damage, rrganisms contrib phosphate
ungi, nematodesd to isolates safrom saline soi
alt Tolerant, Azot
Conference on
1st Century February 2011 tract Book
Page 23
lity of
ur.
well as plant ructure and reduces the bute to soil solublizing , protozoa, alt tolerant il and their
tobacter,
Progressiv
Modeand CDepa
Assessm
Depa
Moriregions of noAmerica. Its pthe removal oorganic polympolymers and hazards to huma natural,cost water of our materials for dseed was extrMoringa Oleifsludge conditmicroorganism.MOCP was fto verify the cthe potential tcost.
Moringa ole
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ment of WaterCo
Jain Ree
artment of Micro
inga oleifera (Mrth-west India a
pods have been eof organics, andmer. Treatment inorganic chemi
man health. So,theffective,eco fricampus. Moring
drinking water trracted and thenifera coagulant tioning propertiems at room temfound to be stablcoagulation actito be used in th
eifera , Moringa
Arts, Science 11005
biology
PP2
r Purificationoagulant Prot
ena R. and Ka
reenajain512@obiology, Moolji
MO) is a multipuand indigenous temployed as an
d coagulant for plants general
icals which are nhe main objectiviendly adsorbentga oleifera seedreatment. The con purified on thprotein (MOCPes as alum. It amperature. It alle at pH 6.8.A smvity experiment
he wastewater tr
Key Wooleifera Coagula
Mi
Published on 25t
21
n Potential oftein (MOCP)
adam Shradh
@gmail.com i Jaitha College,
urpose, mediumto many parts oinexpensive andwater treatmently uses materianot that much cve of this work wt for the examinds were investigoagulant protein he lab scale by P) possessed conalso showed antso inhibits the mall volume coats. We concludeeatment in an e
ords ant Protein (MO
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
f Moringa Ole)
ha D.
, Jalgaon-42500
m or small-sized of Asia, Africa, d cost effective t. It is a non-toxals like synthetost worthy and a
was to use the Mning the quality ogated as alternatfrom the Moringusing dialysis
nsiderable coagutimicrobial effebacteriophage
agulation method that the MO bi
efficient way and
OCP), Coagulatio
Conference on
1st Century February 2011 tract Book
Page 24
leifera
02
tree, from and South
sorbent for xic natural tic organic are causing
MO seeds as of drinking tive natural ga Oleifera technique.
ulation and cts against replication d was used iomass has d with low
on, Alum.
Progressiv
Modeand CDepa
A Cost-Effe
Analysi
* Jogi
* Department
Mushproduced abovcultivated andpopular for thwas used anthroughout. Mobserved wasreported but thof the flower nwas 77 g and tflora of mushmainly includincludes K,Mfurther optiminflorescence
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
fective Protoc
is and High Y
i M. M., Surya
Anandwa
t of Microbiology
hroom is a flesve the ground ond analysed the
heir nutritional vd temperature
Mushrooms wers regarding the he count obtainanormally attainethe dry weight whroom sample. des proteins, car
Mn,Cu,Na,Zn,Ca.mization of cond
and weight of th
Mushroom, F
Arts, Science 11005
biology
OP0
col for Cultiv
Yielding Nutr
awanshi Supr
ni Nilesh P., a
mopa2005@red
gy, PSGVPM’s, A
shy, spore-bearin soil or on agrospecies of Mu
alue. For the cuof 18-320C anre harvested on
inflorescence. able in the preseed is within 60-1was 11 g which w
We have biochrbohydrates, Fat, ( AAS and Fl
ditions which ishe fruiting body.
Key woFungiculture, AA
Mi
Published on 25t
06
vation, Micro
ritious Variet
riya V., More B
and Patil Jaye
diffmail.com ASC College Sha
ing, fruiting boo industrial wastushrooms Pleur
ultivation purposnd humidity of n 21st days. AAn average of
ent study was up00 g. The wet w
was quite satisfachemically analy, amino acids anlame Photometes likely to give
ords AS and Flame Ph
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
bial and Bioc
ty of Mushro
Bhagyashree
esh A .
ahada, 425409, N
ody of a fungutes. In our projeotus Sajar-Kajue. Well sterilize80-85% was m
Another promisif 10-12 flowerspto 20 flowers. T
weight reached inctory. We have dsed the mushrond minerals wher ) .We intende better yield in
hotometer
Conference on
1st Century February 2011 tract Book
Page 25
chemical
ooms.
e V.,
Nandurbar
s typically ct we have
u which is ed substrate maintained ng feature
s has been The weight n this study done micro oom which hich mainly d to go for n terms of
Progressiv
Modeand CDepa
Biotra
P.G. Departm
Relea
toxicity, muta
given in deter
the azo dyes. A
the study of d
2R. In all 12 p
isolated which
medium and
showed decolo
dye was deter
reduced the C
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ansformation
Ka
ravimicro3@g
ment of Microbio
ase of azo dye
agenicity and rec
rmining the abili
Acclimatized mi
decolorization an
promising isolate
h could decolor
up to 68.00 %
orization up to 6
rmined by spect
OD by more tha
Arts, Science 11005
biology
OP0
n of Textile A
ale R. V. and T
gmail.com , prak
ology and ReseaBarshi – 413 4
in the environm
calcitrant nature
ity of microorga
icroorganisms is
nd degradation o
es of Reactive O
ize 1000 μg/ml
in half strength
67.00 % in less th
trophotometer at
an 85.00 %.
Mi
Published on 25t
05
Azo Dyes by A
Thorat, P. R.
kash_micro1@y
arch Center, Shr411, Solapur
ment is of grea
of the dye, cons
anism in decolor
solated from natu
f the dyes React
Orange 16 and 1
of dye to more
h nutrient medi
han 24 hours. Pe
t (λmax) 492 nm
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Aerobic Bacte
yahoo.co.in
i. Shivaji Mahav
at concern due
iderable attentio
rization and degr
ural sources, we
tive Orange 16 a
isolate of Orang
e than 70.00 %
ium. The cell f
ercent decoloriza
m and 484nm. S
Conference on
1st Century February 2011 tract Book
Page 26
eria
vidyalaya,
to, colour,
on has been
radation of
ere used for
and Orange
ge 2R were
in nutrient
free extract
ation of the
Six isolates
Progressiv
Modeand CDepa
Isolation
Ka
1Instit
Campcause of bacbacterium, copoultry, antibiin live poultrybacterium. PhBacteriophagehas been donepoultry in Punstudy 26 isolBiochemical tconfirm themcombat the prspecific.
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
of Bacteriop
amble A.1, De
tute of Bioinform2Department o
pylobacter jejuncterial enteritis ommonly found iotic resistance iy is difficult. An hage-therapy is es specific for the on it in India yene and their perclates of Campytests were carrie
m. Alongside 6 bresence of Camp
Campylob
Arts, Science 11005
biology
PP2phage against
Pun
Souza N.2, Pa
bpkap@unipmatics and Biotec
of Microbiology
ni is a Gram-negworldwide. It in poultry. Due
is rampant in Caincreasing trenda promising a
his bacterium haet. This study focentage reductioylobacter were ed out along wbacteriophages wpylobacter jejun
Key Wobacter, Bacteriop
Mi
Published on 25t
22
t Campylobacne
arkar S.2, Kap
pune.ac.in chnology, Univery, University of P
gative food-bornis a microaerope to the unrestrampylobacter. Bd in antibiotic realternative for Cave been isolatecuses on isolatio
on of target C. jeisolated from sith a latex agglwere isolated fr
ni which were fo
ords phage, Phage-th
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
cter from Pou
padnis B.P.2*
rsity of Pune. PuPune. Pune
ne pathogen that philic and thericted use of antiocontrol of thissistance is obser
Campylobacter ed previously buon of the phages ejuni strains. In tslaughter houseslutination test anrom Chicken Inound to be Cam
herapy.
Conference on
1st Century February 2011 tract Book
Page 27
ultry in
une
is a major rmotolerant tibiotics in
s bacterium rved in this biocontrol. ut no work from retail the present s in Pune. nd PCR to ntestines to
mpylobacter
Progressiv
Modeand CDepa
Isolation
D
FreshIndian subconmicrobiologic(AB), SalmoStaphylococcusamples colleindividual samfor future tesmicrobial constudies. Knowprogramme anare necessary,isolates were susceptibility to be resistantwere resistantemergence of measures. Theprovide an altPractices to cooperation iconsultations resistant patho
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
n and Identifi
*Ketki N
Department of M
h and frozen seantinent. Prawns cal contaminatioonella and Shus aureus, Vibrected from fish mples varied widsting of seafoodntamination of wledge of contamnd effective hyg, should contamicollected from ttest for 16 antibt to 50% of the t to at least one
f new pathogens,ese 12 drug resternative to antibensure pathoge
is essential, andto address the
ogens and the po
Arts, Science 11005
biology
PP2
fication of Pat
Nalawade and
Microbiology, The
afood products a(Penaeus mono
on. Total bacteriShigella spp., rio spp and Psemarkets across
dely. The method, and as a temseafood for comminations is ver
giene as well sanination by colifothese experimeniotics from varioantibiotics used
e drug. The inte, and the need fistant strains we
biotics. These stuen free sea food FAO, OIE a
issues related otential public he
Mi
Published on 25t
23
thogens of Se
d Rajendra Ch
e Institute of Scie
are in wide use odon) were examial counts of aeEnterobacteria
eudomonas spp Mumbai. The m
od used for analymplate for region
mparative epidery critical in thenitary proceduresorm and faecal conts which were tous classes. 12 od. It was found ensification of fifor sustainable trere treated with udies will help tod supply. Inteand WHO have
to antimicrobiaealth impact.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
eafood Penae
houre
ence, Mumbai.
and typical of mined for the perobic mesophil
aceae, Eschericwas done for
microbiological ysis could servenal testing scheemiological ande Good Hygienics in food procesoliform bacteria then subjected toof these isolates w
that 98.81% of fish culture has reatments and pr several herbal
to establish Goodernational interde organized a nal use, the eme
Conference on
1st Century February 2011 tract Book
Page 28
eus sp.
the diet in presence of lic bacteria chia coli,
10 prawn quality of
e as a basis me on the
d statistical c Practices
ssing plants persist. 51
o antibiotic were found the strains caused the rophylactic extracts to d Hygienic
disciplinary number of ergence of
Progressiv
Modeand CDepa
To Study A
Department of
The mliterature. The13 medicinal pand ethanolicmicrobial actidetect the zoGarcinia indicand many comcan prevent thprepared from
Herbal Extra
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Anti-Bacteria
Kh
of Biotechnology
medicinal propee purpose of thisplants and to pre
c extracts of diivity on Blood one of inhibitioca showed maxmmercial toothphe growth of de
m the most effecti
acts, Anti-Bacter
Arts, Science 11005
biology
PP2
al Activity of
Mixed Den
hadpekar A.*,
y, Modern Colleg
erties of several s study is to evalepare a poly herifferent medicinAgar Medium. on. Terminalia
ximum zone of inastes were testeental bacteria anive samples.
Key worial Activity, Ag
Flora
Mi
Published on 25t
24
Various Med
tal Flora
, Thombre R.
ge of Arts, Scienc
plants been docluate the anti-barbal product in thnal plants wereAgar well diffuchebula, Cary
nhibition. The med against mixednd can act as an
ords gar Well Diffusioa.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
dicinal Plants
ce and Commerc
cumented in ancacterial effect of he form of tablee screened for usion method wyophyllus aroma
mixture of effectid dental flora. Tn astringent. Ta
on Method, Mix
Conference on
1st Century February 2011 tract Book
Page 29
s against
ce, Pune 5.
ient Indian extracts of t. Aqueous their anti-
was used to aticus and ive extracts he mixture
ablets were
ed Dental
Progressiv
Modeand CDepa
S
1 D2Head
Laccfound in whitesource would wheat & maizwoody part ofmedia containthe effect of cfiltrate would
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Screening and
Kawade S
Department of Md, Department of
ase are multi ce rot fungi. Laccbe used in place
ze. A purpose of dead plants. T
ning guaiacol. Scculture filtrate ond be detected.
Lact
Arts, Science 11005
biology
PP2
d Production
S.S.*, Diwan V
Microbiology, Daof Microbiology,
opper oxidases case act on broae of glucose & gof this study is tThese isolate woucreened fungi wn dye decoloriza
Key Wotase, Fungi, Dye
Mi
Published on 25t
25
n of Laccase f
V.A.1, Ranjeka
ayanand ScienceDayanand Scien
having wide suad range of subsguaiacol for laccto isolate fungi uld be screenedould be tested fo
ation. Also chara
ords Decolourization
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
from Fungi
ar M.K.2
College, Latur nce College, Lat
ubstrate specifictrates. The prim
case production tfrom industrial
d for laccase profor production ofacterization of th
n.
Conference on
1st Century February 2011 tract Book
Page 30
tur
city mainly mary carbon
that is rice, effluent &
oduction on f laccase & hese culture
Progressiv
Modeand CDepa
Studies on
Departm
Provd
Shivajinagar, bioemulsifier broth. Provden
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Bioemulsifie
Lalit Lande, S
ment of Microbio
dentia stuartiiPune. It showeof 237 EU/ml antia stuartii also
Arts, Science 11005
biology
PP2r Production
from Gard
Smita Bhuyan
lalitlande5532@ology, Modern co
Shivajinagar
was isolated ed good emulsifat 300C, PH 7.5 wo showed produ
Mi
Published on 25t
26 n from Provdeden Soil
n and Shilpa
@gmail.com ollege of Arts, scr, Pune 05
from garden sfication activitywith (0.5%) sun
uction of urease
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
entia Stuartii
Mujumdar
cience and comm
soil of Moderny and produced nflower oil in Lu
and gelatinase.
Conference on
1st Century February 2011 tract Book
Page 31
i Isolated
merce,
n College, maximum
uria Bertani
Progressiv
Modeand CDepa
Compara
1. D
In thbioinoculants for nitrogen fserial dilutionconfirmation oby subjecting bioinoculants Organisms weof the rhizospThe root lengtwith bioinocuincreasing thein soil and alamino acids, sthe environmebe isolated fAlso, such bichemical fertil
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ative Study OSoil A
M
Department of b
his project a prepared from
fixing organism n using Ashby’sof organism. Plathe organism twere prepared o
ere counted by Nphere was more th and the shooulant preparede nitrogen contenso productivity;sugars used by pent giving highfrom the soil aniofertilizers woulizers.
Arts, Science 11005
biology
PP2Of Bioinoculan
And Non-Rhi
M. S. Vrushali2
saivrush.1989@biotechnology, Sp
2. Dr. D. Y. Pati
comparative rhizosphere soifrom rhizosphe agar. Gram sta
ant growth promto phosphate solon ashby’s brothNeubaur’s chamb
active physioloot length of the pd from rhizosphnt of the soil; ph Plant Growth P
plants. As the rhh efficiency in tnd custom-mad
uld not disturb t
Mi
Published on 25t
27 nts Preparedizosphere So
2, N. Rutika1
@yahoo.com picer Memorial il University
study was cil and non-rhizoere and non-rhizaining and Caps
moting rhizospherlubalition using h using suitable ber. Lab trials wogically than thaplants was foundhere. This projehosphate solubilPromoting Rhiz
hizosphere organterms of yield o
de biofertilizers the biological c
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
d From Rhizooil.
College, Pune.
arried out besphere soil. The
zosphere soil wasule staining ware properties wePikovskayas agstrain and kept
were done. The at of non-rhizosd to be more in sect will be applisation increase
zosphere organisnisms are more of crop such orga
can be given cycles as is don
Conference on
1st Century February 2011 tract Book
Page 32
osphere
etween the e screening as done by as done for re detected gar. Liquid on shaker. microbiota
sphere soil. soil treated plicable in phosphate
sms release adapted to
anisms can to farmers.
ne by other
Progressiv
Modeand CDepa
Isolation of
Mad
Depa
Micrwere isolated Screened for hydroxymate positive .Thissiderophore pbroth. It wasEnvironmentaoptimum for m
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
f Siderophor
dhuri Londhe,
artment of Micro
oorganisms fromon Luria agar
sederophore protype of siderophs organism takeproduction was s found that Kal factors pH 5.maximum sidero
Arts, Science 11005
biology
PP2
re Producing of Adathod
, Smita Bhuya
obiology, Moder
m rhizosphere s. Twelve strain
oduction by Arnhore respectivelye for further stcarried out withing’s B Media .6, Temperature
ophore productio
Mi
Published on 25t
28
Organism frda Vasica
an and Dr.Shi
rn College Shivaj
soil sample of Ans were isolatednow and Caskys y. Gram positivetudy ,Media oph Succinate bro
produces maxe 280C, aerationon.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
rom Rhizosph
ilpa Mujumda
ajinagar, Pune -5
Adathoda vasicad. One Gram po
assay for Catace rod showed Arptimization for
oth, Luria broth,ximum siderophn at 120 rpm w
Conference on
1st Century February 2011 tract Book
Page 33
here Soil
ar
5.
a (Adulsa) ositive rod cholate and rnow assay maximum
, King’s B hore. Other were found
Progressiv
Modeand CDepa
Comparati
Mahesh R
1Depart
Obesrisk. The synddeveloping coof abnormal areason could strong associperturbations hot topic of recommunity in16s rRNA geclones were sanalysis conficontrol rats th
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ive Analysis o
R Ghule1, Raje
tment of Microbi2National
sity is defined adrome is just no
ountries and Indiand visceral obenot explain thi
iation between were reported, a
esearch. To comn a rat model of ene based librarysequenced for e
firmed that the an diet induced
Arts, Science 11005
biology
PP2of Faecal Mic
Wistar R
esh M ChomaShouch
iology, Modern Centre for Cell
as abnormal fat ot limited to thea is no exception
esity was reporteis obesity epide
gut microbiotaa keen hunt for
mplement these efobesity. In this
y for control aneach of the fouratio of Bacteroobese rats.
Mi
Published on 25t
29 crobiota fromRats.
l1, Deepak R he2
College, ShivajinScience, Pune-4
accumulation the western countrn. Recently, an aed from one of temic. Recent disa and obesity.an exclusively
fforts, we initiatstudy, we have
nd obese wistar ur animals in eaoidetes to Firmi
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
m Normal an
Patil2 and Yog
nagar, Pune-411411007.
hat imposes a gries, it has sprawalarming rate of the metro cities.scoveries have
Although phyresponsible bact
ted screening guconstructed and
r rats. Approximach group. Bioiicutes is higher
Conference on
1st Century February 2011 tract Book
Page 34
nd Obese
gesh S.
1005
great health wled many prevelance . A genetic revealed a
ylum level terium is a t microbial d screened,
mately, 200 informatics r in gut of
Progressiv
Modeand CDepa
Producti2526)
Ma
The p
xylinum is oncellulose(BC)in release of hNowadays, bahealth care inwhich limits iBC using fruapplication fstandardized iindicate maxim(0.5%). Fruit medium as caCellulose obtamethod. Mix the banana peon the cotton against the con
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
on of BacteriUsing Fruit
nasi karmark
manasi.mkDepartment
population increne of the widely, Although planharmful byproduacterial cellulose
ndustries. Althouits application ouit waste insteafor medical puin synthetic medmum productionwastes (mashed
arbon and nitrogained was quantfruit waste was el. To prove its gauze and chec
ntrol.
Arts, Science 11005
biology
PP3
ial Cellulose Waste and C
kar, Poorva D
[email protected], of Microbiology
ease necessitatesy studied micront are major proucts such as lige finds wide ap
ugh production oon large scale. Inad of synthetic urpose. Carbondium (Hesrin &n of BC obtainedd banana peel angen source for mtified on dry wefound to be mo
application in thcked with respec
Mi
Published on 25t
30
by AcetobactChecking For
abir and Meg
poorva79@gmy, Fergusson coll
s search for newoorganism for thducers of cellulo
gnin which doespplications in paof BC by A. xyln present study media has bee
n and Nitrogen& schramm) for p
d using Fructosend mix fruit wamaximum produeight basis as weore suitable for the health industryct to water abso
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ter Xylinum (Nr Its Applicat
ghana kukarn
mail.com lege Pune
w biopolymers. Ahe production oose, its degradat
s not occur in caaper making, galinum is known
economical proen studied alonn sources werproduction of Be (0.5%) and Yeaste) was substituuction of biomasell as by specifithe production oy, obtained BC worption & vertic
Conference on
1st Century February 2011 tract Book
Page 35
NCIM tion
i
Acetobacter of bacterial tion results ase of BC. arment and it is costly
oduction of ng with its re initially BC. Results east extract uted in the ss and BC. c chemical of BC than was coated
cal wicking
Progressiv
Modeand CDepa
Aureoba
Dep Aure
explored for itorganism alsoand along wipigments callmausonii, has(Vinay Rale) hto study Aureformation on level to compmedium. The batch- scale parameters watemperature, pfrom shake concomitant rmolasses and spent wash is initial BOD a2100000 mg/interesting. Ouor no pigmenproduction asorganism unde
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
asidium PulluC
Meenakshi D
partment Of Micr
obasidium pulluts ability to prod
o produces differith the pullulanled melanin. Ths been studied inhas exhaustive r
eobasidium pullumolasses and sppare its performamount of biofermenter in c
as optimized acpH, etc. . Also, flask to ferme
reductions in BOspent wash fromobtained which
and COD for belit respectively.ur last objective nts which itselfs well as BOD er UV light for 2
Arts, Science 11005
biology
PP31ulans GrownComparative
Dhar, Nasim K
robiology, Fergu
ulans, a yeast-likduce pullulan, a rent types of enz
n which is extrahis organism ann our laboratoryresearch experienulans with the spent wash at shamance (growth mass as well as
compare with scording to the fthe morphology
enter. The secOD and COD v
m brewery industh is 24000 mg/lieet molasses is o The results ofwas to try obtain
f helps better rand COD redu
20 minutes.
Mi
Published on 25t
on Various FStudies
Kherad, Vinay
usson College, P
ke fungus has bpolysaccharide zymes which haacellular; it alsond its close rely for quite somnce with the orgsole objective oake flask and atand product fo
s pullulan produshake flask, whfavor of this orgy change of the condary objectivvalues of thesestry. The initial Bit and 215000 mobtained which f this study of ning an organismesult in extrace
uction. This is d
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Feedstocks fo
y Rale
Pune 411004
been well-studieof multiple uses
ave different acto produces the ative, Aureobas
me time and one ganism. We undef growth and prbatch-scale ferm
ormation) in stauction is quite hhen the fermenganism viz., agitorganism is obsve was to ma substrates, viz.
BOD and COD fmg/lit respectivel
is 280000 mg/lone so far are
m which produceellular polysacchdone by exposin
Conference on
1st Century February 2011 tract Book
Page 36
or
ed and s. This tivities
black sidium
of us ertook roduct menter andard high in ntation tation, served anifest ., beet for the ly and lit and
quite es less haride ng the
Progressiv
Modeand CDepa
BombIt is a large siuses. All partmedicinal purasthama, foodleucorrhoea, mdental caries, compounds frmethanol andorganisms liktyphi, and Caantimicrobial be studied furproduction of
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Antimic
*Mo
*Sinhgad Col
bax ceiba belongized, tall, decidus of the tree suc
rpose. It can be d poisoning, parmenorrhagia, ac
tooth ache etcrom stem and ld water. Effectske Proteus, Pseandida spp. Resactivity of plant
rther for drug debioactive compo
Arts, Science 11005
biology
PP3crobial Effect
onika More, *
llege of Science,
gs to the family uous tree, whichch as stem, leavused in treatmealysis, haemoptycne, bleeding pc. Present studyleaves using sols of these extraeudomonas, Stapsults will pave t extracts as welesigning and planounds.
Mi
Published on 25t
32 t of Bombax C
*Gauri Jadhav
Ambegaon (Bk)
Bombacaceae. h has been founves, root, flowerent of diabetes, kysis of pulmonapiles, diarrhoea,y is concentratelvents such as acts were furthphylococcus authe way towardll as its purificatnt tissue culture
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Ceiba
v
), Pune 41.
It found in tropind to have manyrs, and thorns arkidney stone, coary tuberculosis,, dysentery, skied on extractingdiethyl ether, c
her studied on ureus, E. coli, Sds better understion. Purified pr
e techniques for
Conference on
1st Century February 2011 tract Book
Page 37
ical region. y medicinal re used for onstipation, , influenza, in disease, g bioactive chloroform, pathogenic Salmonella standing of roducts can large scale
Progressiv
Modeand CDepa
Produ
Moksh
AmoIndustrial Micproduction inStreptomyces and was seledifferent mediwas modifiedEffect of diffestudied and PInorganic nitrRNase activityactivity increaselected for Ractivity was detected at 96to RNase actactivity does Purification opurification siwas the first spurification anenzyme was ethey bound to(Superose-12)increased 2.3 f
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
uction and Pu
Streptom
hada Godbole
1Modern Co2Nati
ng the five Stcro-organisms, Nn MGYP (Malvenezualae (NCcted for furtheria components o
d to PG (Peptonerent organic andPeptone was forogen sources rey increased by 4ased by 6.7 tim
RNase productiondetected at 72 hours. DNase a
tivity. Streptomynot require me
of the enzyme since it resulted intep of purificationd enzyme was leluted unbound ao the column. Th) and the final fold at this step o
Arts, Science 11005
biology
PP3urification of
myces venezua
e1, Neelima Ku
ollege, Ganeshkhional Chemical L
treptomyces culNational Chemiclt extract, Glu
CIM-2215) showr optimization son RNase produne, Glucose) med inorganic nitroound to be theesulted in very 4.8 times (270 U
mes (1986U/mg n. The maximum
hours and maactivity was also yces venezualaeetal ions whereshowed that hean loss of RNase aon. Ion exchangeloaded on DEAEand other impurihe enzyme was trecovery was 1of purification.
Mi
Published on 25t
33 f Extracellula
alae (NCIM 2
ulkarni1 and S
hind Road, PuneLaboratory, Pun
ltures from NCcal Laboratory,
ucose, Yeast exwed maximum Rstudies. In optimction was studiedium which is gen sources on R
e most suitable poor RNase ac
U/ml to 1302 Uto 13285U/mg)
m extracellular Raximum intracel
observed but it e produces non e as DNase actat treatment is nactivity. Ammone chromatographE cellulose (anioities were removthen loaded on F0%. The specif
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ar RNases fro
2215)
S. S. Deshmu
e 411053. ne
CIM (National Pune) screened xtract, PeptoneRNase activity (mization studiesed and the MGYsimple and costRNase productio
organic nitrogctivity. In PG m
U/ml) where as th. Hence this me
RNase activity anllular RNase acwas very less asspecific nuclea
tivity requires Mnot a suitable mnium sulphate prhy was used in non exchanger) coved from the enzFPLC gel filtratific activity of th
Conference on
1st Century February 2011 tract Book
Page 38
om
ukh2
Centre for for RNase
e) medium (270 U/ml) s effect of
YP medium t effective. on was also gen source. medium the
he specific edium was nd specific ctivity was s compared ase. RNase Mn2+ ions. method for recipitation next step of olumn. The zyme since ion column he enzyme
Progressiv
Modeand CDepa
Isolation S
Optimizat
Neha Nai
1
Penicacylases beloacylase proteiconnecting the( 6-APA) corpenicillin’s .PIsolation of 1Hindustan AmicroorganismPenicillin–V serratia marcphysiologicalATUAVP184FAME analysof ATUAVP bacillus spp.,method (CLUproduction ofCultural Conproduction wOptimum inoobserved at 3volume was (2.0%) max. glucose folloPVA producti
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Screening fo
tion of Cultu
on Produc
k1, Avinash S
1Modern college2Nati
cillin-V acylase ong to N-terminins , amidohydroe side chain to thrresponding sideenicillin acylase
10 morphologicAntibiotic pums was carried o
acylase activitcescens, compal characterizati46 was performesis, isolate was 1846 was comp, and a phylogUSTAL X) wif PVA by B.cernditions and Ewere studied inoculum size wa0°C and pH 7.075ml in 250 mPVA producti
owed by sucrosion is achieved
Arts, Science 11005
biology
PP3
or Penicillin-V
ure Condition
ction of Enzy
Sunder1, LaxPund
e of Arts, Scienceional Chemical L
(PVA) is a pharnal nucleophile olase enzymes their B-lactam nue chain. 6- APAe acts as scavengcally different oune, Screeningout by plate assayty. Activity waaratively resistaion of the Ped by a private iidentified as B
pared with all otgenetic tree waith the related reus. FermentatEffect of Medn batch fermes found to be 1
0, after an incubml Erlenmeyer fion is achievede, and Out of in the case of a
Mi
Published on 25t
34
VAcylase Pr
ns, Effect of
yme from B.
mi Nair1, Vaile2
e & Commerce PLaboratory, Pun
rmaceutically im(Ntn)-hydrolase
that cleave peniucleus, releasingA is the buildinger enzyme for porganisms was dg for penicily method. Out oas checked by nt to penicillin
PVA-producing institute, using
B. cereus. The 1ther known 16Sas constructed
taxa. This is tion parametersdia Supplemenentation under 10%. Maximumbation of 24 hrs,flask. Out of ad in the case all used ‘N’ so
ammonium phos
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
roducing Org
Media Cons
Cereus
idehi Dande1
Pune 411 007, ne
mportant enzymee super family,icillin’s at the a
g 6- aminopenicilg block of sem
phenylacylated cdone from soil lin acylase-
of which 1 isolasensitivity of
n. The morpholoGram positiv
16srRNA seque16S rRNA gene
S rRNA gene sequsing Neighbothe first repo
s such as Optimnts for Maxim
shake flask cm enzyme produ, and optimum d
all used ‘C’ souof glycerol folources used (0.sphate.
Conference on
1st Century February 2011 tract Book
Page 39
ganism,
tituents
& A.V.
. Penicillin Penicillin
amide bond llianic acid
mi synthetic ompounds. sample of producing
ate showed 6-APA to ogical and ve isolate encing and e sequence quences of ur joining
ort on the mization of mum PVA conditions. uction was dispensing urces used llowed by .3%) max.
Progressiv
Modeand CDepa
CharacAntibiotic
Neha S.
Acinepathogen. A cAIIMS7 is muconsists of fweight (20 kbwas phenotypresistance to harbouring thePhenotypic chmethods. In biby SDS PAGEdemonstrated Activity staintype isolate Aof the beta molecular chaweight plasmi
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
cterization of cs of Acinetob
Vora, Shradh
Departme
etobacter baumaclinical isolate fultidrug resistantfour plasmids, o) and two low mpic and biocheβ-lactam antibioe high molecula
haracterization oiochemical charE followed by acpresence of caring showed that
AIIMS 7 (19.31klactamase (39.4
aracterisation of id is under study
Acinetobacter,
Arts, Science 11005
biology
PP3
Plasmid Encbacter Bauma
ha B. Nadhe, RParde
karishma@unent of Microbiolo
annii is an oppfrom All India It showing high rone high molec
molecular weightemical characterotics. A transfoar weight plasmif β-lactamases wacterization molctivity staining wrbapenemase andt of the two typ
kD and 39.44kD44kD) which af the beta lactamy.
Key Wo
Multidrug Resis
Mi
Published on 25t
35
coded Resistaannii AIIMS
Riddhi J. Shaesi *
nipune.ac.in ogy, University o
portunistic nosocInstitute of Medresistance to β-lular weight (57t (<10 kb each).rization of the rmant Acinetobaid was obtained was performed blecular weight ofwith nitrocefin. Pd AmpC type ofpes of beta lacta
D), the transformappears to be p
mase gene/s prese
ords
stant, Plasmid, β
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ance To β-LaS7, A Clinical
h and Karishm
of Pune
comial multidrudical Sciences, Nlactam antibiotic7 kb), one mid The objective ofHMW plasmid
acter baylyi ADby natural trans
by using variousf β-lactamase wa
Phenotypic charaf β-lactamase inamases present imant T17 showeplasmid encodeent on this high
β-lactamase.
Conference on
1st Century February 2011 tract Book
Page 40
actam l Isolate
ma R.
ug resistant New Delhi, cs. AIIMS7
molecular f this study d encoded
DP1 (T17), sformation. disc assay as detected acterization n AIIMS7. in the wild d only one
ed. Further h molecular
Progressiv
Modeand CDepa
Optimiz
Neelim
Modern
34 yefruits, fermenextract Peptonand best ethansingle parametemperature aproduced maxnitrogen sourincubation for10.659 g/L reof optimized m10.659 g/L oEthanol was psource, 10 g/fermentation from 6.12 g/Las estimated bPG-2, SCJ-3 acharacterizatioTorulaspora d
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
zation of Gro
a Kulkarni*,
College of Arts,
east cultures wernted foods, sugane (GYP) mediunol producing cetric studies. Efand fermentationximally using 20ce at pH-4, temr BG-1 and 24 hospectively as estmedium for SC
of medium consproduced maxim/L urea as nitrtime. During o
L to 11.2 g/L whby Gas chromatand BG-1 were on and identifdelbreucki respec
Arts, Science 11005
biology
PP3
owth Paramet
Yeas
Shreyas KumHussain Kho
Science and Co
re isolated fromar syrups and um. All the isolacultures BG-1, Sffect of differenn time on ethan0 g/L sucrose asmperature 30 °Cours for SCJ-3 wtimated by Gas J-3 was estimat
sumed. This givmally for culture
ogen source, ptimization stud
hen estimated bytography. The thcharacterized by
fied as Kodamctively.
Mi
Published on 25t
36
ters for Etha
sts
mbhare, Panduokhawala
mmerce, Ganesh
m different sugar agricultural wasates were screenSCJ-3 and PG-2nt carbon sourcnol production s carbon source C, inoculum sizwith ethanol conchromatographyed and the yieldves 92.64 % efPG-2 using 20 gpH-5, temperatdies ethanol cony CAN method ahree best Ethanoy morphology, b
maea ohmeri,
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
anol Producti
urang Lahare
hkhind, Pune 41
containing foodstes using Glucned for ethanol 2 were selected ces, nitrogen sowas studied. Et, 10 g/L cocon
ze 5 % (v/v) inncentration of 7.y. The total carbd of ethanol profficiency of ethag/L fructose as tture 37 °C andncentration wasand 2.242 g/L tool producing yeabiochemical andCandida interm
Conference on
1st Century February 2011 tract Book
Page 41
ion by
e and
1053.
d materials, cose Yeast production for further
ources, pH, thanol was
nut meal as n 58 hours 77 g/L and
bon content oduced was anol yield. the Carbon
d 48 hours s increased o 5.180 g/L ast isolates
d molecular media and
Progressiv
Modeand CDepa
CompaGlorios
Dep
Glorithe alkaloids fof gout and rmicro organisThus, taking irespect, an atpotential of thmethanol and Escherichia cPseudomonas clinical isolatKlebsiella, Psactivity againformulate a nbased on syneMethanolic exand inhibited gThe highest screening of ecurrent investiis the first reporganism by t(Kalhari).
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
arative in-vitrsa Superba ag
Clinical
Nikita L
partment of Mic
iosa superba (fafrom the plant (Crheumatism. Thsms has stimulainto account thettempt was madhis plant. Extrac
their antimicrobcoli, Staphyloco aeruginosa, Prtes of Eschericseudomonas and
nst cephalosporinew, cost effectiergistic activity oxtract was foungrowth of the cesynergism rate extract showed igation leads to port in vitro whthe extracts of m
Arts, Science 11005
biology
PP3
ro Antimicrogainst LaboraIsolates of C
L. Lad and Ra
crobiology, Instit
amily: Liliaceae)Colchicine and Ge increasing preated the explorae medicinal impode in the currect of dried leavebial activity wasoccus aureus, Sroteus vulgaris, chia coli (3 std Acinetobactern resistant orgaive antimicrobiaof cefotaxime wid to act synergi
ephalosporin resiwas attained apresence of alkfresh sources ofhich revealed symedicinally imp
Mi
Published on 25t
37
obial Activityatory and Anommon Path
ajendra G. Ch
tute of Science, M
) is widely usedGloriosine) are bevalence of muation of alternatortance of Glorint study to inv
es and tubers was determined aSalmonella typhBacillus sp .andtrains), Proteusr. The extract aanisms. The objal combination ith methanolic existically with thistant organisms against Proteus kaloids, flavonoif new antimicrobynergistic inhibiportant ornament
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
y of the Extrantibiotic Resishogens
oure
Mumbai 400032
d as a medicinalbeing used in theltidrug resistantte antimicrobialiosa superba Linvestigate the anas extracted in eagainst standard hi, Klebsiella pd 8 cephalosporis mirabilis, Enalso showed anject of this stufor drug-resistanxtract of Glorios
he cephalosporinin presence of cmirabilis. Phy
ids and steroids.bial regimes in fition of antibiotital plant Glorios
Conference on
1st Century February 2011 tract Book
Page 42
act of stant
l plant, and e treatment t strains of regimens. nn., in this
ntimicrobial ethanol and cultures of
pneumonia, in resistant terobacter,
ntimicrobial udy was to nt diseases sa superba. n antibiotic cefotaxime. ytochemical . Thus, the future. This ic resistant sa superba
Progressiv
Modeand CDepa
Ext
Niruta Y. K
1Modern col AmoIndustrial Micactivity (257Uextract 0.3%,optimization sand Yeast extrwas useful in(Beef extract and inorganicRNase activitnitrogen sourcmaximum RNfold increase profile of Strpextracellular Rproduced in 92081) produccould be deteenzyme showsuitable methchromatographpH 7.0. Enzym0.5M salt resuloaded on FPL
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
tracellular RNStrep
Killedar1*, An
llege, Ganeshkhi
ng five Streptocroorganisms) S
U/ml) during scr Peptone 0.5%studies first efferact when omitte
n reducing cost o0.8%, Glucose 1
c nitrogen sourcty than inorganice for RNase pro
Nase activity at pin RNase activ
ptomyces sp.(NCRNase was prod
96 hrs. with 76%ced nonspecific ected in absencewed that heat trhods which rehy the enzyme cme bound to CMulting in 3 fold LC gel filtration
Arts, Science 11005
biology
PP3
Nase producteptomyces sp.
nupama Sonaw
ind, Pune 41105
omyces culture Streptomyces spreening in MGY) medium so sct of various meed from MGYP mof the medium. 1%) medium insces showed that ic nitrogen souroduction. Effect pH 6.0 in BG mity and 6.6 fold
CIM 2081) cultuduced in 72 hrs
% sugar utilized wnuclease produ
e of metal ions, reatment and Asulted in verycame unbound wM-cellulose colupurification andcolumn (Supero
Mi
Published on 25t
38
tion and puri(NCIM 2081
wane1 and Su
53. 2National Ch
from NCIM .(NCIM 2081)
YP(Malt extract selected for optedia componentsmedium higher aThe organism
stead of MGYP organic nitroge
rces. Beef extraof initial pH on
medium. Optimizd increase in spure showed that s. and maximumwithin 72-96 hrsucing DNase ati
but Mn+2 ion fAmmonium sulphy poor recoverywhen loaded on Dumn at pH 5.0 ad 16.6% recoveryose 12) resulting
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ification from1)
umedh S.Des
hemical Laborato
( National Colshowed maximu0.5%, Glucose
timization studies was studied. Mactivity was obtacould grow in smedium. Effect
en sources prodact was the moRNase producti
zation studies repecific activity.
in BG medium m intracellular Rs. Streptomyces ivity also. RNafor DNase. Purihate precipitatioy. During ion DEAE-celluloseand eluted with y. CM eluted enin 8.9% recover
Conference on
1st Century February 2011 tract Book
Page 43
m
hmukh2
ory, Pune
llection of um RNase 1%, Yeast es. During
Malt extract ained. This simple BG of organic
duced more ost suitable ion showed esulted in 2 Production maximum
RNase was sp. (NCIM
ase activity ification of on are not
exchange e column at buffer and
nzyme was ry.
Progressiv
Modeand CDepa
Isolati
P.S, Pa
Kidnworld. Most osalt found in containing elemetabolize diurine, formingby gastrointebacteria were of morphologbelongs to genof different oFTIR analysicoAtransferasTitrimetric anto be around 4
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ion and Ch
atil , Patil Sha
ney/ urinary tracof the stone are c
variety of foodevated amount oetary oxalate. O
g insoluble calciustinal tract micisolated from di
gical and biochemnus- Stomatococoxalate sources. s, which suggee by the organisalysis with KMn
40%.
Arts, Science 11005
biology
PP3aracterizat
Organ
am A , Bedse,
K.T. H. M. Coll
t stone disease composed of oxad. Increased absof oxalate is tak
Oxalate in humanum oxalate and ecroorganism etcfferent sources. mical observatioccus . This organ
Oxaltate degraest the productiosm. The amount nO4. The degrad
Mi
Published on 25t
39 tion of Oxalnism
,Tanuja D, Ja
lege, Nashik
is a major healtalate or calciumsorption of oxalken. Humans lans can be eliminexcretion in faecc. Oxalate degrOrganisms were
ons. Among thenism is further cadation product,on of oxalate dof oxalate degraation rate of oxa
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
late Degrad
adhav Sandip
th problem throm oxalate. Oxalic
late mayoccure ack the enzyme nated through exces and oxalate drading facultative characterized ose organism one
characterized forformate was d
degrading enzymadation was detealate by the isola
Conference on
1st Century February 2011 tract Book
Page 44
ding
p R .
ughout the c acid or its
after food needed to
xcretion in degradation ve aerobic on the basis e organism r utilization detected by me oxalyl-ermined by ate is found
Progressiv
Modeand CDepa
Comparamarces
Departme
Prodi
reported charaanticancer actmedium to simproduction waair and rhizoswas selected fSerratia marcenvironmentaland purificatiosupplemented pigment than medium whenused as colora
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ative Study oscens Using V
Pankaj .
ent of MicrobiolShivaji
igiosin producedacteristics of havtivity. From an imultaneously enas the aim of thiphere and screefor further studiecescens by API l parameters usion protocol was
with fatty aciddefined media
n compared withants in textile and
Prod
Arts, Science 11005
biology
OP1on The ProduVarious Crud
Applica
.S. Picha & Sh
pankaj.picha@logy, Modern Coinagar, Pune-411
d by Serratia maving antifungal, industrial point
nhance the growtis work. Pigmenned for their abies. It was biochekit. Production
ing crude and palso modified an
d containing soa. Crude carbonh pure carbon soud paint formulati
Key Wo
digiosin, Serratia
Mi
Published on 25t
13 uction of Prodde Fatty Acidations
heetal .P. Par
@gmail.com ollege of Arts, Sc1005, Maharash
arcescens is a primmunosuppresof view the nec
th of Serratia mnt producing orgility to produce emically charact
n was optimizedpure carbon sournd developed to
ource were founn sources reducurce and the pigions and also for
ords a, Pigment, Pain
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
digiosin by Sed Sources and
rdeshi
cience and Commhtra.
romising drug ossive, antiprolifecessity to obtainarcescens and th
ganisms were isopigment. One is
terized and identd with respect trces. Extraction
o recover pigmennd to give bettece the cost of gment thus obtair therapeutic app
nt.
Conference on
1st Century February 2011 tract Book
Page 45
erratia d Its
merce,
owing to its erative and
n a suitable he pigment olated from solate, PP1 tified to be to different procedure
nt. Medium er yield of production
ined can be plications.
Progressiv
Modeand CDepa
O
Poojari P
D Acineserious therapof these organof these organdiversity of anfrom various assays and alschromosomal GenElute Bacprimers specifClass D), amishowed positifor Class C ßADC type of ßto be more preThe resistanceare highly usepresent on porganisms. Hview.
A
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Oligotyping oClinical
ritika, Ratnak
Department of Mi
etobacter spp. hpeutic failure. Ranisms have allownisms in the hospntibiotic resistanhospitals in In
so harbored multor plasmid enc
cterial Genomic fic for differentnoglycosides anve amplification
ß-lactamase folloß-lactamase wasevalent among ae of Acinetobacted as a treatmelasmids, it willence this study
Acinetobacter, ß-
Arts, Science 11005
biology
PP4of Antibiotic l Isolates of A
karan Neena,Pardesi Ka
karishma@un
icrobiology, Uni
has emerged as iapid genotypingwed a better apprpital environmennce in twenty-sindia, which shotiple plasmids. Tcoded. GenomicDNA kit. PCR
t classes of ß-lacnd quinolones. On with more thanowed by Class As found to be moaminoglycosides ter to quinolonent now-a-days. l lead to transwill prove to b
Key Wo-lactamases, Am
Mi
Published on 25t
40 Resistance G
Acinetobacter
, Mehta Khyatarishma*
nipune.ac.in iversity of Pune,
important nosocog methods for threciation of the
nt. In the presentix isolates of Ac
owed multidrug The genes for thec DNA was isol
amplification wctamases (Class
Out of twenty-sixn one primer. MaA, Class D andore prevalent. Siand gyrA, parCs is of prime imIf the genes fofer of these gebe important in
ords minoglycosides an
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Genes from r Spp.
ti, Shirode Ab
Pune 411 007
omial pathogense identification epidemiology ant study, we characinetobacter sppresistance by p
e drug resistancelated using Sigm
was done using 3s A, Class B, Cx isolates characaximum isolates
d Class B. The Aimilarly aph-A6
C, QnrA among qmportance since
r quinolone resenes to other
n epidemiologica
nd Quinolones
Conference on
1st Century February 2011 tract Book
Page 46
bhinav,
s causing a and typing nd survival acterize the p. obtained phenotypic e are either ma-Aldrich 30 pairs of lass C and
cterized, all s had genes AmpC and was found
quinolones. quinolones istance are susceptible al point of
Progressiv
Modeand CDepa
Optimi
Depart
Four agar from rhiNagar, Pune 0further study Three differenmedia 2 were these three mparameter likeand F2 showe320C respectivisolates.
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ization of Am
Pradnya N. tment of Microbi
different Amylaizosphere soil o05, after primary(F1 & F2) whicnt media viz. schecked for the
medium sabourae pH and tempered optimum provely. Time cours
Arts, Science 11005
biology
PP4mylase, Produ
Rhizosphe
Nadivkar andiology, Modern C
ase producing oof Botanical gary screening on stch were showinsabouraud, Amyeir ability to suppaud showed marature were optiduction of amylse of amylase pr
Mi
Published on 25t
41 uction by Funere Soil
d Sheetal. P. College, Shivajin
rganisms were irden of Moderntarch agar plate.
ng zone of clearylase specific mport maximum a
aximum productimized using sablase at pH 7 androduction was al
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ngi Isolated f
Pardeshi nagar, Pune 411
isolated on potatn College of AC
Two fungi werrance on starch
media 1, Amylaamylase producttion of amylasebouraud. Fungald 6, temperaturelso determined f
Conference on
1st Century February 2011 tract Book
Page 47
from
1005
to dextrose CS, Shivaji re taken for agar plate. se specific tion, out of e. Physical l strains F1 e 280C and for both the
Progressiv
Modeand CDepa
Redu
P
Rapid
wastewater inconsumers. Swastewater trpollutants, whcharacteristicsalso on the efhealth organizto heavy metareduces the edirectly or inddisorders suchheart disease, depletion of thheavy metals bioremediationsludge to redcontained 4.2obtained fromin 24 hours. Senvironmentalincubation timat ambient teconcentration copper from d
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
uction of heav
Prajakta Bhag
Department o
d population grn agriculture. Waludge is compo
reatment processhich usually conss of sludge depefficiency of its trzation (WHO) esal contaminationefficacy of meddirectly linked toh as arthritis, asarterial sclerosi
he body's immucan be reduce
n of domestic duce heavy met1- 6.43 mg/l co
m sludge, four isotudies on reductl factors such as
me. One of the isemperature (28±
and incubationdomestic sludge.
Arts, Science 11005
biology
PP4vy metals from
Microorg
gwat, Priya B
bendrepriya61@of Microbiology,
rowth, urbanizatastewater poses osed of by-prods. It contains bosist of heavy metend on the origireatment, followstimates that 80%n, as it produce
dical treatments o many complicasthma, chronic is, multiple scle
une system. It haed by using bacsludge to reduc
tals content wasopper and 6.0-8olates could remtion of copper frs temperature, polates, R, showe
±2oc), pH 7, 1%n for 28 hours,
Mi
Published on 25t
42 m domestic s
ganisms
Bendre and Vr
@gmail.com Fergusson Coll
tion has increascertain environ
ducts collected oth compounds tals, organic polinal pollution lo
wed by the treatm% of all chronic es free radicals.
by up to 60%ations such as hefatigue syndrom
erosis, Parkinsonas been reportedcteria and fungce its toxicity. s studied. Sludg.15mg/l zinc. A
move up to 54.76rom sludge were H, carbon sourced optimum redu% glucose conproving to be u
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
sewage by usi
rushali Patil
lege, Pune
sed the need fonmental and heal
at different staof agricultural
llutants and pathoad of the wastement of sludge. disorders can bePresence of hea. Heavy metal eadaches, anger
me, diabetes, fibn's disease, ulced that the conce
gi such as AspeHence, bioremege was neutral
Among the twen6% copper and 2 carried out with
ce and nitrogen uction of copper ncentration, 0.1%useful in the re
Conference on
1st Century February 2011 tract Book
Page 48
ing
or reuse of lth risks to ges of the value and
ogens. The ewater and The World e attributed avy metals toxicity is etc. and to
bromyalgia, ers etc. and entration of ergillus for ediation of
in nature, nty isolates 2.11% zinc h respect to source and up to 71%
% peptone eduction of
Progressiv
Modeand CDepa
IsolatProducing
Priya
Departm
1 g osterile saline. was spread oncolonies wermorphologicastaining and twithout tryptoproduction. AIAA productioThe effect of checked by Ptryptophan (1Absorbance wincubation byby using trypMaximum IApH 8. P2 wastest.
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ion and Charg Organism fr
anka Mane, S
ment of Microbio
of soil sample coSerial dilutions
n sterile Luria –Bre selected anl features ,Gramthen for IAA prophan. Red colo
All showed IAA on than others. Cf time course anP2 and P4 isolat
mg/ml). Uninowas checked at y replacing carboptone as nitrogeA production w
s identified as M
Arts, Science 11005
biology
PP4racterizationrom Rhizosp
Smita Bhuyan
ology, Modern C
ollected from rh of the sample
Bertanni agar pland screened fom character ,motiroduction by Salour formation wproduction. Out
Cultures were prnd carbon and ntes by Salkovasoculated LB bro540 nm. Maxim
on source (yeasten source. Effe
was seen at 37°CM. lacunata and
Mi
Published on 25t
43 n of Indole Acphere of Gram
n and Dr. Shil
College, Shivajina
hizosphere of grawere prepared aates. After incubor preliminary ility, oxidase teslkovaski’s test. M
was considered pt of 8, P2 and Preserved on LB nitrogen sourceski’s test. Mediaoth with tryptopmum IAA was t extract) from Lct of temperatu
C for P2 isolate P4 as unidentifi
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
cetic Acid (IAm (Cicer Arie
lpa Mujumda
agar, Pune – 05.
am was mixed iand 0.1 ml of thbation at 30⁰C fo
characteristics st, catalase test aMedia used waspositive evidencP4 isolates gave agar slants for fs on IAA produa used was LB phan was kept
produced at 72LB broth with sure and pH waand 32°C for P4
fied organism by
Conference on
1st Century February 2011 tract Book
Page 49
AA) entinum)
r*
.
n 10 ml of his dilution or 24 hrs ,8
by their and capsule s LB broth ce for IAA
maximum further use. uction was broth with as control. 2 hours of ucrose and s checked. 4 isolate at y mini API
Progressiv
Modeand CDepa
Heali
doctors; this pbacteria. Henevidence that an alternate fpotency of cohoney againstthe above honwas found thaspores. All the10% v/v concrest. Candida sugar solutionproperties of hfound that all sugar solutioncontent that aagainst the labof the same omethod. It waThe effect of hinfected burnscolorimetric mhoney was slFurthermore tallantoic memfound that hona negative conof the factors t
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Wound
Purnim
Department o
ing of infected problem has recence new approahoney helps in a
form of treatmeommon honeys ft S. aureus, P. aneys was carried at Khadi honey we honeys were thcentration while persisted beyond
n with concentrathoney were solethe four organis
n indicating thaaccount for its loboratory strain oorganism were as observed thathoney and SSD s) on preformed measurement of lightly more efto test the effe
mbrane assay waney accelerated tntrol and a salinethat make honey
Arts, Science 11005
biology
PP4
d Healing Pro
ma Ranawat
of Microbiology,
and chronic wently escalated daches for treatinaccelerating wouent. A study wafound in the marerugenosa, Strepout using agar dwas most potenthe most effectivit took a conce
d 30% v/v concetion of sugars cloely due to the ossms mentioned sat honey has soower MIC’s. Sinf Pseudomonas tested for their
t honey inhibitedointment (a combiofilms of S. abiofilms using c
ffective than SSct of honey ons performed usinthe rate of angioe control. This sy more effective
Mi
Published on 25t
44
operties of ho
and Jyoti Ma
Sophia College
wounds is a comdue to the persistng wounds are und healing and tas conducted to rket namely- Daptococci and Cadilution method t and had the lea
ve against pseudoentration of at leentration of honose to that in honsmotic effect of survived beyondome properties nce honey was faeruginosa, four
r sensitivity to hd these strains a
mmon antibiotic cureus and P. aer
crystal violet. It SD on biofilms n wound healingng fertilized Legogenesis as compsuggests that angin wound healin
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
oney
ntri
e, Mumbai.
mmon challengetence of antibiot
required. Therthus should be loevaluate the an
abur, Pondaghat andida albicans.(range 5%-30% ast amount of coomonas, knockineast 25% to kno
ney. A control Mney was set up t
f the sugars presd 30% concentraother than its hfound to be mosr antibiotic resishoney using agat a concentratiocream used on wruginosa was evwas seen that 5of the above
g, angiogenesis ghorn chicken egpared to a positigiogenesis may ang.
Conference on
1st Century February 2011 tract Book
Page 50
e faced by tic resistant re is some ooked at as ntibacterial and Khadi
. MIC with v/v) and it
ontaminant ng it out at ock out the
MIC using a to see if the sent. It was ation of the high sugar st effective tant strains
gar dilution on of 15%. wounds and valuated by 0% diluted organisms. by chorio
ggs. It was ive control, also be one
Progressiv
Modeand CDepa
Optimi
D The
biotechnologicdiscover and dneed of time from stagnantisolate was isindicated by revealed glucsource and RTshaking condoptimized parrecovery of supernatant oprecipitation acharacterizatioThe enzyme wstimulated enzgood organic to be effectiveexhibited goodthe best subscommercial dImmobilizatioactivity was slipase was foudetergent. Alsdegraded the days.
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ization and CSelected
Rahu
Department of M
growing interecal applications develop new comthe present wo
t fish runoff wasolated on Victzone of hydrolose, ammoniumT (28⁰C ± 2⁰C)ition for favorarameters and feculture medium
obtained by cenand dialysis. Furon of lipase. Optwas found stablzyme activity upsolvent enhancine stimulator for d stability with bstrate, among tdetergent which
on of lipase was studied where 2und efficient in cso the enzyme fat completely.
Arts, Science 11005
biology
PP4Characterizat
Bacillus sp. a
ul Brid and Ra
Microbiology, The
est in lipase that the enzyme
mmercial microbrk deals with liater and its chatoria Blue Olivelysis of olive o
m chloride, olive) as optimum temable lipase prodermentation studm for extractionntrifugation wasrther, cell free sutimum enzyme ale in wide rangep to 493.33%. 1ng the lipase actlipase activity
bleach i.e. Sodiuthose tested. Th was even mcarried in differ% agarose had
complete removawas found to hThe lipase hydr
Mi
Published on 25t
45 tion of Lipaseand Its Appli
ajendra Chou
e Institute Of Sci
production is e present and sobial sources for liipase productionaracterization ane Oil Agar wheoil. Optimizatione oil, potassiummperature with
duction. Productdies revealed thn and purificats further purifieupernatant was uactivity was obsee of pH and tem1% iso- propyl ativity up to 166.6increasing it up
um HypochloriteThe lipase activmore enhancedrent carriers by ethe highest spec
al of olive oil frohave antagonistirolyzed castor o
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
e Production ication
ure
ience, Mumbai
related to theo there is immenipase productionn Bacillus specind application were lipase produn of medium c
m chloride as anoptimum pH of
tion was carriedhat 50 hours wation of lipase. ed by ammoniuused as enzyme erved at pH-7.0 mperature. Potaalcohol was fou66%. Tween-80 to 173.33%. Th
e. Olive oil was fvity was foundd in presence entrapment and cific activity. Thom cotton fabric ic effect on beeoil more than 89
Conference on
1st Century February 2011 tract Book
Page 51
by a
e potential nse need to n. With this ies isolated wherein the uction was omponents n optimum f 7.0 under d out with as best for
Cell free um sulfate source for and RT0C. ssium ions
und to be a was found
he enzyme found to be
d stable in of CaCl2.
its specific he purified along with
ef fat as it 9.28% in 6
Progressiv
Modeand CDepa
Laboratory
Depa
Polluthreatened hepollutants. BeSurfactants ihydrocarbons.show limitedbiodegradableTherefore in tand asses its contaminated isolate was semulsificationcarbon sourceThe selected iwith glucose. using Acetoncontent usingcomparative acarried out. 6comparison wobserved usinabout greater (Tween- 80). surfactant in b
B
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
y Study on Bi
Ra
artment of Micro
ution caused byalth and sustain
eing water insoluincrease the a. However chemd efficiency. Oe, less toxic, canthe present studyrole in the biowith crude oil aselected on then assay. It was ses (sucrose, glucsolate was foundThe biosurfact
ne precipitation g Folin Loweryanalysis of purifi61.53% biodegrawith 55% for g whole biosurfareduction in theThus, this study
bioremediation.
Biosurfactant, Di
Arts, Science 11005
biology
PP4ioremediatio
from a G
khi Tari and S
obiology, The Ins
y the release nability of the euble, diesel oil aaqueous solubil
mical surfactants On the whole n be prepared ey an attempt waoremediation of and it’s by produe basis of its screened for thecose and lactosed to be Pseudomtant was extractmethod and an
y and Phenol ied biosurfactantadtion was founTween- 80 wh
factant producer.e surface tension
y revealed the ef
Key Wo
iesel oil, Chemic
Mi
Published on 25t
46 n of Diesel O
Garage
Shilpa Sabnis
stitute of Science
of petroleum henvironment. Dalso shows low lity and consare relatively tox
biosurfactants easily and showas made to isolat
diesel oil. On ucts, six isolates emulsification
e better emulsifie) using the sammonas which gavted from cell frnalyzed for its Sulphuric acid
t with chemical sd in presence o
hereas only 42. The biosurfactan in comparisonfficacy of biosur
ords cal Surfactant, B
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Oil Contamin
s
e, Mumbai 40003
hydrocarbons hDiesel is one of
and slow biodegequently degraxic, less biodegr
are specific w widespread apte a biosurfactanscreening garagwere obtained aactivity measurcation activity i
me oil emulsificave maximum emuree broth of Pse
protein and camethod respe
surfactant (Tweeof purified biosu.85% biodegradant was also founn with chemicalrfactants over th
Bioremediation
Conference on
1st Century February 2011 tract Book
Page 52
ated Soil
32
has always the major
gradability. adation of radable and in action,
pplicability. nt producer ge samples and the best red by oil in different ation assay. ulsification eudomonas arbohydrate ectively. A en- 80) was urfactant in dation was nd to bring l surfactant
he synthetic
Progressiv
Modeand CDepa
Ravindr
Departm
Gibb various develproduction of prime importaPresent work paddy field saproducing cullike pH and te
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Production
ra Baviskar, S
ment of Microbio
berellin (GA) islopmental procef gibberellin andance and it mafocuses on prod
amples. Spectrostures in Czapek
emperature and id
Gi
Arts, Science 11005
biology
PP4n of Gibberell
Sunny GaikwaBhuya
*smitabhuyan@
logy Modern colShivajinagar Pu
a plant hormo
esses in plants. Cd its enhancemenay also meet theduction of gibberscopic analysis rDox broth at 29dentification of t
Keibberellin, hormo
Mi
Published on 25t
47 lic Acid by So
ad, Shilpa Muan*
@gmail.com llege of Arts, Sciune 411 005
one that regulateConsidering its wnt by sources like ever increasinrellin by four direvealed four pr90C for 7 days. Othe fungal cultur
y Words one, Fungi, Czap
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
oil Fungi
ujumdar and S
ience and Comm
es growth and wide array of apke fungi and bacng demand of gfferent fungi iso
romising fungal Optimization of res is in process.
pek Dox
Conference on
1st Century February 2011 tract Book
Page 53
Smita
merce,
influences pplications, cteria is of gibberellin. olated from gibberellin parameters .
Progressiv
Modeand CDepa
Prevalenc
Dep
Staphpart of humancommon causmortality rategolden periodstrains. Severaa semi synthStaphylococcunosocomial pareplaced by epidemiologicand thereforePrevalence ofand environmsurveillance iMicrobiology months were were also isolresistance. Ousamples 106 29.4%) were 2.8% were MstaphylococciCoNS.
MR
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ce of MethiciPatil Hospit
Reen
partment of Micr
hylococci are ubn flora. The mostse of bacterial e from staphylod was soon interal different classhetic penicillin us aureus was athogen worldw
a more stablcal pattern and ie very difficult f multidrug resi
ment can be a cis important as
laboratory of Dstudied for Me
lated from hospiut of 150 Meth(70.6%) were iMR-CoNS. Am
MR-CoNS. Fromwere isolated, o
RSA, Clinical sam
Arts, Science 11005
biology
OP0illin Resistantal and Resea
na A. Dighe an
drmrsdighe@redrobiology D. Y. P
biquitous in naturt virulent of the infection. Afterococcal infectiorrupted by the apses of antibiotics
was introducereported in 19
wide, popularly de drug Oxacilis well known to
to treat, especstant Staphylococause of outbres a preventive Dr. D.Y.Patil hethicillin resistanital personnel anhicillin resistantdentified as Sta
mongst the hospm the air samout of which, 21
Key Womples, Hospital p
Mi
Published on 25t
02 t Staphylococ
arch Centre, K
nd Saral Ghos
diffmail.com Patil Medical Co
re with about a genus, Staphylo
r the discovery ons dropped drppearance of pes were developeded in 1959. F61 in UK. Sin
designated as MRllin. Staphylocoo develop resistcially those conocci amongst peak of infection
measure. Clinhospital. Kolhapnt staphylococc
nd environment at staphylococciaphylococcus aupital personnel
mpling of the h1.66% were MR
ords personnel, Hospi
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ccus aureus inKolhapur
sh
ollege, Kolhapur
dozen species ocococcus aureus,
of penicillin inramatically. Howenicillin resistantd in response. MFirst Methicillinnce then it becaRSA. Methicillinocci spp. has tance to antibiotntracted in the atients, hospital
n in the hospitaical samples re
pur within a percus isolates. Stapand studied for Misolates from t
ureus i.e. MRSA13.33% were Mhospital environRSA and 6.94%
ital environment
Conference on
1st Century February 2011 tract Book
Page 54
n D. Y.
r.
ccurring as is the most
n 1940 the wever, the t S. aureus
Methicillin , n resistant ame major n was later
a unique tics rapidly
hospitals. l personnel al. Regular eceived in riod of 12
aphylococci Methicillin he clinical
A and 44 ( MRSA and nment 204 were MR-
t.
Progressiv
Modeand CDepa
ProductiInd
1. Lecturer in
2.Hea
Xanththickening ageis its ability toquantity of guXanthomonaswas able to prcontaining banother ingrediepolymer mat milled to give
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
on of Xanthadustrial and K
Repe A. B., 1
n Postgraduate D
ad, Department o
han gum is polyent and stabilizeo produce a largum. Xanthan gucompestris is th
roduce extracellunana peel extracents. After fermbe precipitated a powder that w
Arts, Science 11005
biology
PP4an Gum By XKitchen Was
1Dhutekar S.
Department of M
Latuof Microbiology
ysaccharide used er. One of the mge increase in vium helps to crehe pathogen isoular polysaccharict as sugar sourcmentation that c
from the mediuwill be readily so
Mi
Published on 25t
48 Xanthomonasste as a Produ
S., 2Dr. Ranje
Microbiology, Daur Dayanand Scien
as food additivemost remarkable iscosity of a liqueate pleasant texolated from the iides when inocuce, cabbage extrcan vary in timum by the addi
oluble in water.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Compestris uction Media
ekar M. K.
ayanand Science
nce College, Lat
e, commonaly usproperties of xa
uid by adding a xture in many iinfected leaves o
ulated in fermentract as nitrogen
me one to four tion of alcohol,
Conference on
1st Century February 2011 tract Book
Page 55
Using a
e College,
tur
sed as food anthan gum very small ce creams. of cabbage tation broth source and days. The
, dried and
Progressiv
Modeand CDepa
SynthesiApplicatio
Rutuja
Departme
The ato 100 nanomof nanoparticZnO nanopacharacterizatioray diffractionwell diffusiontransmission textiles. Appnanoparticles future aspect o
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
is and Characon on Textiles
a R. Phatate,
ent of Biotechnol
application of nameters is an eme
les is an area of articles were synon was done usinn. The antimicron technique. Aand personal p
plication of efto textiles for
of this project.
ZnO Nanopar
Arts, Science 11005
biology
OP1cterization ofs as Antimicr
Rebecca S. T
logy, Modern CoShivajinagar, P
anoscale materiarging area of naconstant interestnthesized usingng UV spectroscobial activity of An awareness protection has lffective antibacproducing antim
Key Worticles, Antimicr
Mi
Published on 25t
12 f ZnO Nanoprobial Agents
Thombre and
ollege of Arts, ScPune 411 005
als and structureanoscience and nt for its wide rang simple chemcopy, Scanning eZnO nanoparticof personal sa
led to the deveterial agent an
microbial and U
ords robial agent, UV
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
particles and s and UV Abs
Sonal P. Bath
cience and Comm
es, usually ranginanotechnology
nge of applicatiomical method electron micrococles was studied anitation, contaelopment of annd UV absorb
UV absorbing te
V absorbers
Conference on
1st Century February 2011 tract Book
Page 56
Their sorbers.
hija
merce,
ing from 1 . Synthesis ns. and their
opy, and X-using agar
act disease ntimicrobial bers- ZnO extiles is a
Progressiv
Modeand CDepa
Candida G
Lecturer in De
Candeukaryotic, bublastoconidia.pseudohyphaeof normal florHowever, folspectrum anticaused after cgroups over pathogen, seveknown to ctransplantationconcern for CFluconazole adifficult to trefrom a deep -Physicians woof these drugAmphoterecininfection. Heruncontrolled Dand histopathoGram stain anbudding yeastagar. The absappearance ofrapid and cosCandida specinfections.
Candi
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Glabrata Infec
epartment of Mi
dida glabrata, fudding yeast, usu It is the onl
e. Candida glabrra of healthy indlowing the widfungal therapy, colonization by the past few yeral other speciecause opportunn, meningitis, pCandida glabratand can developeat. For this rea- seated source (ould tend to use gs. Patients witn-B, since this re is presentedDiabetes mellituologically as “sund Lactophenol t cells. Yeast colence of pseudoh
f dark pink to vist- effective diacies should be
ida glabrata, CH
Arts, Science 11005
biology
OP0ction of the N
Repo
Roma A. C
neetiroma@gcrobiology., Dr
Institute, Kformerly called ually round to ovly species of rata was considdividuals and rardespread use of
the frequency oCandida glabrat
years. While Caes like Candida tnistic infectionspneumonia and ta is that it is p resistance to son, it is clinica(i.e fluid or tissuan Azole agent
th Candida glais the only eff
d the case repous, having an inuspected mucormcotton Blue moulonies were seenhyphae and chlaiolet colonies on
agnosis of Candperformed on a
Key WoHROMagar Cand
Mi
Published on 25t
01 Nasal Septumort
Chougale
gmail.com .D. Y. Patil Med
Kolhapur Torulopsis glab
val in shape. It mgenus Candidaered as relativelrely causing ser
f Immunosuppreof mucocutaneouta has significanandida albicans tropicalis, C.kruss in septicemihaematologic mless susceptibleother Azoles. H
ally important thue) be fully specfor treatment dubrata should b
ffective therapy ort of a 58 yeanfected nasal sepmycoses”. Micrount of colonies
n on Blood agar amydospores onn CHROMagar Cdida glabrata. Sall clinical spec
ords dida Medium, Da
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
m – An Unusu
dical College and
brata is small umultiplies by proa that does noly non-pathogenrious infections iessive therapy wus and systemicntly increased in
remains the msei and C.glabraia, urinary tra
malignancies. The to AmphotereHence these infehat a pure growciated. Without ue to the decreasbe immediately
for Azole-resisar old male paptum, diagnosed
oscopy of infecterevealed small 3and Sabouraud’
n Dalmau plate cCandida medium
Screening for nocimen suspectin
almau Plate cultu
Conference on
1st Century February 2011 tract Book
Page 57
ual Case
d Research
unicellular, oduction of ot produce nic and part in humans. with broad c infections n older age major yeast ata are now act, organ he primary ecin B and ections are th of yeast speciation,
sed toxicity placed on
stant yeast atient with d clinically ed tissue by 3-5 µ, oval ’s Dextrose culture and m offered a on-albicans ng mycotic
ure
Progressiv
Modeand CDepa
Biological
D
The ivarious areas effect on varionanoparticles be toxic, flamattempt was mgreen synthesAzadirachta inand their antibEscherichia caureus and SPrince Aly Kcharacterized impregnated ostudied. The eantibacterial psheet coated wbactericidal tomaximum ansynergistic babiologically nosocomial in
Silver napneumonia, P
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Synthesis of
*Sab
Department of M
integration of naof medical scien
ous microorgani(SNPs), when sy
mmable and not amade to formulasis of SNPs, wndica and Caricabacterial activitycoli, Klebsiella
Steptococcus pyoKhan Hospital. S
by uv-vis spectronto cotton clotheffect of consecproperty was alswith SNPs could o the isolates an
ntibacterial activactericidal effect synthesized SN
nfections.
anoparticles, AntPseudomonas ae
Allium cep
Arts, Science 11005
biology
PP4Silver NanopAntibacteria
ah Shaikh an
Microbiology, the
anomaterials witnce. Silver has bsms present in mynthesized by wat all environmete a cost effectiv
which were syna papaya with Ay was studied us pneumonia, Pogenes obtainedSNPs (1:2) showroscopy and scanh and their antibutive washing oo investigated. Tbe retained up t
nd of all differevity. These plan
with AgNO3. TNPs incorporate
Key Wotibacterial, Planteruginosa, Staphpa, Azadirachta
Mi
Published on 25t
49 particles andal Activity
nd Shilpa Sabn
e Institute of Scie
th biology is finbeen long recog
medical and induwet chemical reduent friendly. Theve and environm
nthesized using AgNO3 solutions sing disk diffusioPseudomonas ad from bed sheewing maximum nning electron mbacterial activityof the coated disThe antibacterialto 3 washings. Sient plant extractnt extracts wer
This work demoned in cotton b
ords: t extracts, Escherhlococcus aureusindica, Carica p
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
d Evaluation o
nis
ence, Mumbai.
nding wide applgnized as havingustrial processesuction method arerefore in presenment friendly tec
extracts of Allin varying ratio
on method on isaeruginosa, Stapets of casualty wantibacterial act
microscopy (SEMy on the same issks with distilledl activity of the ilver nitrate on itts, Carica papayre further foundnstrates the possbed sheets to
richia coli, Klebs, Steptococcus ppapaya.
Conference on
1st Century February 2011 tract Book
Page 58
of Their
icability in g inhibitory . But silver re found to
nt study, an chnique for lium cepa, s (1:2, 1:4)
solates viz., phlococcus ward from tivity were
M) and then solates was d water on cotton bed ts own was ya showed d to show sible use of
minimize
bsiella pyogenes,
Progressiv
Modeand CDepa
Study of O
Dep
Contalbumin preseby many ocuvarious MPS across MumbVarious isolatProteus vulgasamples. Variantimicrobial diffusion andstudied.Antiadcoverslip adhadherence of asolutions ReNWhere Flexileaffectivity of tcleaning devic
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
cular Microfof MPS
*Sag
partment Of Mic
act lenses have pent in tears are foular organisms. T
against these isbai and 50 samtes viz. Staphyloaris, Klebsiella ious multipurposactivity was studd Agar well dhesive activity
herence method all isolates wereNu and Compleens and ReNu sthese MPS was ce.
Arts, Science 11005
biology
PP5flora, AntimiS Solutions on
gar Vandra &
sagarvandra@crobiology, Instit
proved to be useound to provideTherefore attemolates. A survey
mples were colleococcus aureus. pneumoniae, P
se solution useddied against thesdiffusion methof these isolateand by scanninfound to be dec
ete were found showed maximafound to increas
Mi
Published on 25t
50 crobial and An Ocular Isol
& Shilpa Sabn
@gmail.com tute Of Science, M
eful for visual coa good environm
mpt was made toy was carried ouected from peopStreptococcus m
Pseudomonas sppd for lens cleaninse isolates using hod. Their pee was studied bng electron miccrease in presencto show maxim
al antiadhesive pse when used in
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Antiadhesivelates
is
Mumbai 400032
orrection but lysment for biofilmo study the micut at 17 differenple between 12mutans, Candidap. were detecteng were assessemodified ISO 1
ercentage inhibby contact lens croscopy. The sce of MPS. Out mum antibacteriaproperty. The anpresence of vib
Conference on
1st Century February 2011 tract Book
Page 59
Activity
2
ozyme and m formation croflora on nt opticians 2-49 years. a albicans,
ed in these ed for their 4729, Disc
bition was adherence,
strength of of all MPS al activity. ntiadhesive
bration type
Progressiv
Modeand CDepa
Identificat
1Dr
Tobaand healthy toPolyacrylamid(Enzyme Linkthe presence otransferred oninterest. LaterGel Electrophand thus creat
ToMV, SDS
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
tion and Extr
Samridd
r. D. Y. Patil Inst
acco mosaic viruobacco leaves ade Gel Electrophked Immunosorbof viral protein. nto Nitrocellulosr the genomic D
horesis. Certain ve a new hybrid p
S-PAGE, ELISA
Arts, Science 11005
biology
PP5raction of TobMolecular T
dhi R. Sharma
titute of Biotechn2INDAM, Ba
us is found in toare analysed by horesis). The totbant Assay), whe
The protein is se Membrane. T
DNA is extractedviruses such as Tplant with health
Key WoA, Genomic DNA
Mi
Published on 25t
51 bacco Mosaic
Techniques
a1 and Aparna
nology and Bioinangalore
obacco plants. ISDS-PAGE (S
tal viral protein ere the color chaelectrophoresed This gives the pd (Genomic DN
ToMV are very ehy factors.
ords A, Nitocellulose
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
c Virus (DNA
a Das2
nformatics, Pune
In this project thSodium Dodecyl
is separated usiange in ELISA p
and the proteinposition of prot
NA Extraction) beasily isolated an
Membrane, Aga
Conference on
1st Century February 2011 tract Book
Page 60
A) Using
e
he infected l Sulphate-ing ELISA
plate shows n bands are tein of our by Agarose nd removed
arose Gel
Progressiv
Modeand CDepa
Phyloge
1Dr
The (insulin) in siNCBI (NationMus musculCaenorhabditalignment waprotein sequenpfam, embossspecies relatiostudies of diff
Phylogenetics
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
enetic Analysi
Sand
r. D. Y. Patil Inst
project focusesix different specnal Centre of Biolus, Gallus gatis are sequencas done and phnce was constucs, cath, target p,on on the basis ferent species.
s - to show evolu- cattle, Gall
Arts, Science 11005
biology
PP5is of Insulin (
Too
eep Gajbhiye
titute of Biotechn2Hydera
s on the phylocies the query sotechnology Infoallus, Drosophced using the hylogenetic analcted. the tools u hyperchem, heof insulin struc
Key Woutionary relationslus gallus - roste
Mi
Published on 25t
52 (Protein) Usils
e1, Mr. Sunaka
nology and Bioinabad
ogenetic and stequence of thes
ormation). The sphila melanogas
tools of bioinlysis was perfo
used for the analx, signal p. are cture. It can be
ords ship, Mus muscur, Caenorhabdit
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ing Bioinform
ar2
nformatics, Pune
tructural study se species was tpecies such as Bster, Rattus nnformatics. Theormed dendrogrlysis are blast, wused and is comused for the ta
ulus - house rat Btis - worm
Conference on
1st Century February 2011 tract Book
Page 61
matics
e
of protein taken from
Bos Taurus, norvegious, sequence
ram of the workbench, mpared the axonomical
Bos Taurus
Progressiv
Modeand CDepa
Microbi
Sayali Ma
Microbial Nitro
highly energeEnvironmentaenvironment developed in(FOX-7). It iinsensitive exacetamidiniumwhich on nitrwith two nitWastewater gFOX-7 in highlike methaemenvironmentalobtained by sproduction sitisolate of Micwhen incorporconcentration from 20% to 4were also ablthus can be us
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
al Degradatio
ahajani, Abhij
l Sciences Divisi
oexplosives cometic materials. Ually transformedand human bei
nsensitive high is one of the imxplosive (IHE). m chloride with ration followed tro and two amgenerated duringh amounts and itmoglobnaemia. lly transformedsoil enrichment e. Out of these f
crococcus sp. corated in synthetiof FOX-7 as 40
40% after supplee to grow in th
sed for bioremed
Arts, Science 11005
biology
MPPon of Diamin
Nitroexp
it More, Seem
ion, MACS-Agha
mprise a major Use of nitroexpld products of ings. Present stu
energy nitroexmportant nitro eIt is synthesizediethylmalonateby hydrolysis gmino groups a
g the productiont appears to be aTherefore bio
d metabolites istechnique using
four bacterial isoould use FOX-7 ic medium at th0 mg/l in the synementation of me wastewater ge
diation purpose.
Mi
Published on 25t
P53 nodinitroetheplosive
ma Sarnaik an
arkar Research I
component of losives is impor
these nitroexpudies include bxplosives 1,1-dexplosive of clad by HEMRL Pe to obtain 2-megives FOX-7. It attached to carbn of FOX-7 is hallergic to humandegradation stus necessary. 13g the soil samplolates comprising
as the sole sourhe concentration nthetic medium,
medium with 0.01enerated during
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ene, a High E
nd Pradnya K
Institute, Pune 4
nitro compoundrtant for nation’plosives are h
biodegradation odiamino-2,2-dinitass called as hiPune, by the treethyl-pyrimidineis an aliphatic
bon-carbon douhighly acidic ann beings causingudy of FOX-73 bacterial isolles collected frog 3 Actinomycetrce of carbon anof 500 mg/l. Atthe removal wa
1% peptone. Theproduction of F
Conference on
1st Century February 2011 tract Book
Page 62
Energy
Kanekar
11 004
ds and are ’s security. harmful to of recently troethylene igh energy eatment of e-4,6-dione compound
uble bond. nd contains g symptoms 7 and its lates were om FOX-7 tes and one nd nitrogen t the initial s enhanced ese isolates FOX-7 and
Progressiv
Modeand CDepa
Microbial
Snehal Bar
Microbial
Chemhowever, theydue to large vchemicals eithEffluent treatindustry. Sincsmall scale unfor cluster of in nature. It imicrobial cultchemical induMagenta, CryTurbuphos, Hhydrochloridewastewater is matter. It conextent of 800was developedbacterial consfrom an initialscale in the lafrom initial 68pilot plant of sSphingomonasFlavobacteriuBacteroides, Rin reducing thpurpose.
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
BioaugmentCommon E
ri, Nilesh Sonu
Sciences Divisio
mical industries y also contributevolume of efflueher in the form tment plant thuce it is difficult nit, existence of such small scales quite difficult ture. The wastewustries producinstal violet, Acid Hexaconazole) e, Cyclosporin),
highly acidic inntains organic lo0 mg/L and amd using the bactortium used at sl 8000 mg/l to 1
aboratory. The ex800 mg/l to 396size 25 m3. The s, Comamonas
um, Aeromonas,Ralstonia and Ache organic load
Arts, Science 11005
biology
OP1ation for Tre
Effluent Trea
une, Amit SinP. P. Kan
on, MACS - Agh
play an importe to environmenent generated duof unused raw
us becomes an to set up an effcommon effluen
e units. The wasto treat such ty
water under studng dyes and pyellow, Fast gar, pharmaceutica, petrochemicaln nature and darkoad in terms of
mmoniacal nitrogteria isolated frosite. The bacteria1500 mg/l at flasxperiments carri7 mg/l at 25 l scbacteria belonges, Cupriavidus, Bacteroides, chromobacter, a
d and thus indic
Mi
Published on 25t
10 eatment of Inatment Plant
nnarkar, S. S.nekar
harkar Research
tant role in devntal pollution p
uring the producmaterial or unreunavoidable c
fluent treatmentnt treatment planstewater in CETPype of mixed effdy is from a CETpigments (e.g. Drnet GBC); pestals (Bitamethazls (spray oil, lk in appearance Chemical Oxyg
gen up to 900 mom the soil in thal consortium desk level, and 550ied out at site recale and from 30ed to genera Ochs, Xanthomona
Ralstonia andall other genera cated their pote
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ndustrial Effl(CETP)
Sarnaik, G. K
Institute, Pune 4
velopment of anarticularly wate
ction and presenecovered finishe
component of aplant independ
nt (CETP) becoP is usually hete
ffluent using a sTP receiving efflDirect blue, Yticides (Temephzone valerate, liquid paraffin)with appreciable
gen Demand (COmg/L. Bacterial che premises of Ceveloped lowere00 mg/l to 1820 esulted in remov000 mg/l to 140hrobactrum, Pseas, Klebsiella, d Achromobacte
were found to bential for bioaug
Conference on
1st Century February 2011 tract Book
Page 63
uents in
K. Wagh,
411004
ny country r pollution ce of toxic ed product. a chemical dently for a mes useful erogeneous ingle, pure luents from
Yellow FG, hos, Ethion,
Fluoxetine ) etc. The e settelable OD) to the consortium CETP, and d the COD mg/l at 5 l
val of COD 0 mg/l at a
eudomonas, Bacillus,
er. Except be efficient gmentation
Progressiv
Modeand CDepa
DetermSa
Sh
1Department
Listertype of sanitizmonolate ). Lwhich helps bGG3V & BHhypochloride tween80 and Ethidium bromfor Mdrl geGentamycin, R
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ination of Reanitizers and
hahina Shaikh
t of microbiology
ria spp are ubiqzers and antibioListeria spp. couacteria to increaH2V were scre, dettole and amremarkable shi
mide agar plate ene. Both the Roxythomycin, A
Tween80
Arts, Science 11005
biology
PP5esistance of Ld Antibiotics
h, Abhay Raor
y New Arts Com
quitous in nature tics in the preseuld secreat extr
ase the unsaturatieened for deter
mpicillin. MIC weft was observedassay. Further cisolates showi
Azithromycin.
Key wo, MIC, Efflux pu
Mi
Published on 25t
54 Listeria monocin Presence o
rane and Dee
mmerce and Scien
& express the rence of tween 8racellular enzymion to saturationrmination of reere determine bed. Efflux pumpchecking of effluing susceptibilit
ords ump, Mdrl gene,
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
cytogens Towof Tween 80
epali Giremar
nce College, Ahm
esistance toward0 (polyoxyethel
me i.e glycosyl n ratio at 370c. Twesistance towardefore and after tr activity was cux was confirmety towards Am
, AST.
Conference on
1st Century February 2011 tract Book
Page 64
wards
mednagar
ds different lin sorbitan transferase wo isolates ds sodium reatment of checked by ed by PCR mpicillin ,
Progressiv
Modeand CDepa
Antibac
Shi
Department
Growantimicrobialscoriander, nucumin, turmeron black pep(Curcuma lonand phytochepepper (seeds)and DMSO. Sbacterial patho
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
cterial ActivitBorne
ikha Malla, Sh
t of Microbiology
wing concern abs. Spices are theutmeg, cinnamonric, ginger exhibpper (Piper nignga.). Crude extrmical screening) and turmeric (
Spice extracts inogens.
Antibacteria
Arts, Science 11005
biology
PP5ty of Crude E
e Pathogenic M
hilpa Mujumd
y Modern colleg
bout safety of fe most commonn, lemongrass,bit antimicrobialgrum L.) cuminracts were used
g. Solvent extra(rhizomes); solven methanol & eth
Key woal activity, Piper
Mi
Published on 25t
55 Extracts of SpMicroorgani
dar and Madh
ge of Arts, Scienc
foods led to thnly used antimic
cardamom, blal activity. Presenn (Cuminum cy
for determinatioction was doneents used were mhanol showed an
ords nigrum, Curcum
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
pices against sms
huri Amrutkar
ce & Commerce,
he development crobials. Spices ack pepper whint investigation yminum L), andon of antibacter for cumin (seemethanol, ethanntibacterial activ
ma longa.
Conference on
1st Century February 2011 tract Book
Page 65
Food
r
Pune 05
of natural like clove, ite pepper, is focused
d turmeric rial activity eds), black ol, acetone
vity against
Progressiv
Modeand CDepa
Evalua
Dep
A hatheir microbiacomes in varithe distinctivelaboratory. Suuseful in foodartificial synthHence the prenatural pigmewere isolated maintained bytests: macroscLeifsons, Casetheir pigment nutrient brothbetter pigmenisolate no-26 biomass produ200rpm at thewas at 120th hpigment produpresence of giron.The pigmactivity of pigcosmetic prodagainst E.coliobserved agaiusing human Rto check the ntest. Since pigbe used to disin beverages, c
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ation of AntibP
Sh
partment Of Mic
allmark feature oal colonies whenety of hues and e colour of theuch pigmentatiod, cloth, paintinghetic pigments esent work mainents from natural
from soil, watey refrigeration atcopic and microein hydrolysis, Iproduction abil
h media by specnt in King’s A m
and isolate nouction of isolate
e interval of 24 hhour followed buction determine
glucose as a C-sment was extractgment extract waducts by well dii, K.pneumonaeinst S.pyogen anRBC’s to check
non-toxicity of thgment showed a scover new drugcakes or to deco
Arts, Science 11005
biology
PP5bacterial ActiPseudomonas
ilpi.R.Chand
shilpichand@crobiology, Instit
of several pathogn propagated in
has A hallmarkeir microbial co
on comes in varg, cosmetics, pha
which create senly focuses on tl resources. Theer and contamint 40c. The isolateoscopic morphondole and H2S plity in King’s Actrometric meth
medium compareo-15). The direce no-26 in Kinghours was studieby decrease afteed for isolate noource, ammonia
ted by Blackwooas tested against iffusion techniqu, P.vulgaris, S.fnd P.merabilis. Tthe suitability of
he pigment whicgood antibacteris with broad spe
orate and display
Mi
Published on 25t
56 ivity of Pigmeaeruginosa
& R.B.Vaidya
@yahoo.in tute Of Science,
genic microbes the clinical labo
k feature of seveolonies when priety of hues anarmaceuticals anerious environmthe production ae pigment produnated egg sampes were identifieology motility, production. The iA, Demoss and od at 520nm. O
ed to Demoss &Fct co -relationshg’s A broth at 3d and the maximr 144 hours. Th
o-26 were pH-7.a as a N- sourceod and Neish methe common patue. The antibacflexnerri, S.aureThe hemolytic af pigment for hu
ch was confirmedial activity and nectrum or it can the food items.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ents Produce
a
Mumbai 400032
is the distinctiveoratory. Such pigeral pathogenic mpropagated in thnd has often prond plastics as anment and safety and evaluation o
ucing Pseudomonles on cetrimide
ed by performingCatalase, Oxidaisolates were comFranks, Minima
Only, two isolatFranks broth mehip between pig
350C on rotary smum pigment anhe optimum con0 and temperatue and Fecl3 as aethod and the anthogens of food,terial activity w
eus; while no acactivity test was uman consumptiod by negative reno blood hemolybe used as a foo
Conference on
1st Century February 2011 tract Book
Page 66
ed by
2.
e colour of gmentation microbes is he clinical oven to be
n option for problems.
of safe and nas species e agar and g following ase, Hugh-mpared for al Salt and tes showed edium (i.e.: gment and
shaker with nd biomass nditions for ure-35oC in a source of ntibacterial , water and
was noticed ctivity was performed
on and also sults of the ysis, it may od colorant
Progressiv
Modeand CDepa
Biodegra
S
Hexais produced aleather tannersources and exceeding theother organs. genome. It microorganismreduce it to tPseudomonas,identify microwater. The samost of the inmicrobes had and analysed ffound that onlthen subjecteMingioli Synteffect of pH checked to opsewage watersto a concentrappm in 2 and was found ouwas 37 ºC andremediating csuch high conorganism is m
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
adation of He
Singh Viveka ,
avalent Chromius a by-product ories etc. Due tocontaminate th EPA standards It can also lea
affects plant ams, a large numthe non toxic T, Arthrobacter
oorganisms that aamples taken wendustrial effluent
the highest degfurther. The conly one isolate waed to increasingthetic medium anand Temperatuptimize the pars. Results showeation of 50 ppm5 days respectiv
ut to be 6 to 8. Td degradation wacontaminated sitncentrations and mesophilic.
Arts, Science 11005
biology
PP5exavalent Ch
, Srivastava S
Fergusson Co
um is a toxic, muof many industr
o its high solubihem. Consumpti
can lead to sevead to the develalso. Although mber of them caTrivalent Chrom
etc. The aim oare able to degraere sewage watets are discharge iradation potenti
nsortium was plaas responsible fog concentrationsnd the reduction
ure on the degrarameters for raped that the isolat
m with complete vely. The optimuThe optimum teas faster at 55 ºCtes due to its abalso its removal
Mi
Published on 25t
57 romium Usin
Shreya and Go
llege, Pune
utagenic and carcries like metal pility, it can reacion of Hexavaere damage to thopment of canc
Hexavalent Can tolerate high
mium. These incof our research ade high levels oer so as to obtain sewage. The al for Hexavalen
ated to obtain theor the degradatios of Hexavalenn was checked eadation potentiapid remediation te could degrade
and rapid degraum pH range of mperature for th
C. This isolate cobility to reduce from sewage w
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ng Microorga
ore Suneeti
cinogenic compolating, dye manch the undergro
alent Chromiumhe kidneys, lungcers and mutati
Chromium is hh concentrationslude genera likis to isolate, anf Hexavalent Ch
ain an indigenousample whose cnt Chromium we individual isolaon process. This nt Chromium ineach day up to dal of the isolate
of contaminatee Hexavalent Chradation of 10 ppthe isolate for d
he growth of theould be extremel
Hexavalent Chater treatment pl
Conference on
1st Century February 2011 tract Book
Page 67
anisms
ound which nufacturing, ound water
m at limits gs, skin and ons in the
harmful to s and even e Bacillus, nalyse and hromium in us strain as consortia of as selected ates. It was isolate was n Davis –
day 10. The e was also ed sites or romium up pm and 20
degradation e organism ly useful in
hromium at lants as the
Progressiv
Modeand CDepa
Soura
D
Silvereduction, andobserved usinanoparticles 420nm form (EDX), ScannThe nanopartbacteria and gwas studied on
Silver nano
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Study of S
bh Mehta*, R
Dept. of Biotechno
er nanoparticlesd chemical reduing UV-Visiblewas studed by the UV-Vis sp
ning Electron mticles of Silver dgram positive ban cancerous cell
oparticles, UV-V
Arts, Science 11005
biology
PP5Synthesis of S
Rebecca Thom
ology, Modern C
s were prepareuction method. Te absorption s
the typical surfpectrum. We haicroscopy (SEMdemonstrated anacteria . The antiline The Human
Key Wo
Vis spectrum, SE
Mi
Published on 25t
58 Silver Nanop
mbre and Shu
College, Shivajin
d by bacterial The formation ospectroscopy. Tface Plasmon abave used energy
M) to characteriznti bacterial activi cancerous activn Monocytic cell
ords EM, EDX, Antib
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
articles
ubhangi Puran
nagar, Pune 05
reduction, Bioof Silver nanopaThe formation bsorption maximy dispersive sp
ze the Silver nanvity against gramvity of Silver nal line (THP1).
acterial, Antican
Conference on
1st Century February 2011 tract Book
Page 68
nik.
o physical articles was
of Silver ma at 418-pectroscopy noparticles. m negative
anoparticles
ncerous.
Progressiv
Modeand CDepa
Decoloriza
Dep
Disch
aquatic ecosyeffluent bio tdegradation odecolorizing bBurkholderia aeration, % icarbon and nconditions reqyellow, whichculture was oDecolorizationshowed high disappearancedecolorizationdecolorizationwell-documenlemon yellow extract. The idyes. Adsorpstudied. Cells(30%) as comon alginate bcomparable. Poriginal dye. PTriticum aestiheight of shoo
Direct l
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ation of Texti
*Sunil. S.
partment of Micr
harge of textile stems and humatreatment proce
of dyes to non tbacterium was i
cepacia by 16inoculum, initianitrogen sourcequired for maxih was used as a observed at pH n was confirmed
peak at the e of peak and an is due to dn took place strinted reports. Bur
in presence of solate had an ab
ption of Direct ls immobilised in
mpared to free celbeads (11%) thPhytotoxicity andPhytotoxic effecivum was studiedot and length of l
lemon yellow, D
Arts, Science 11005
biology
PP5
ile Dye DirecCepac
Tiwari and Sa
robiology, Instit
wastewater contan health. This esses to producoxic metabolitesisolated from dy6S rRNA sequal concentration s was studied imum decolourimodel dye. The7.4 and incuba
d by UV-VIS spwavelength of
appearance of pdye degradationictly under aerobrkholderia cepa1% (w/v) glucosbility to decolorlemon yellow onn 7.5% Na-algills (77%) and cohe efficiency ofd microbial toxicct of Direct lemod by calculating leaf.
KeywoDecolorization, B
Mi
Published on 25t
59
ct Lemon Yelcia
angeeta. K. J
tute of Science, M
taining toxic dyestudy is part of
ce reusable wats by bacteria. Inye contaminated
uencing. The efof dye, initial with an aim t
ization and dege optimum dye-dation temperaturpectrophotometef 410nm. The peak at 298nm, n by Burkholdbic conditions, w
acia showed 99%se and 98% in prize five other stn Na-alginate benate matrix sho
onsidering the dyf the two systecity studies wereon yellow on grofactors, such as
ords: Burkholderia cep
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
llow by Burkh
angam
Mumbai 400 032
es can adverselyf efforts to deveter by decolorizn the present stud sample and idffect of pH, te
concentration to determine th
gradation of Dirdecolorizing actire of 30o C after. The initial dydecolorized dywhich indicate
deria cepacia. which is contrar
% decolorizationpresence of 1% (tructurally differeads and degradowed lower decye adsorption phems was not foe also carried ouowth of Phasealpercentage of ge
pacia, Phytotoxic
Conference on
1st Century February 2011 tract Book
Page 69
holderia
2
y affect the elop textile zation and udy, a dye
dentified as emperature, of various
he optimal rect lemon ivity of the ter 7 days. ye solution ye showed ed that the
Optimum ry to other n of Direct (w/v) yeast rent textile
dation were colorization henomenon ound to be ut using the lus mungo, ermination,
city.
Progressiv
Modeand CDepa
PhytocheLeaves of B
*S.B.Dah
1 Departmen
2 Departm
Phycarbohydrateschloroform exracemosa Linmethod againStaphylococcuaeruginosa, Sand Proteus vagainst enteriacetate extracAntibacterial attempt to deenteric infectio
Antib
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
emical and AnBauhinia Rac
hikar1, 2, S. A.
nt of PharmaceuEduc
ment of Microbio
ytochemical scs, alkaloids, flavxtract, ethyl acenn. were preparenst certain enus aureus, KlebsSalmonella typhvulgaris. The Mic bacterial pathct were slightlyactivity of variovelop a new phon.
bacterial activity
Arts, Science 11005
biology
PP6ntibacterial S
cemosa Lam. Bacterial P
Bhutada2, D.
sbdahikar10@tical Microbiolocation and Resealogy, Sant Gadg
reening of thevonoids, steroid
etate extract andd and antibacter
nteric bacterial siella pneumoni
himurium, SalmoMethanol extractshogens than they higher antibaous extract of leaharmaceutical dr
Key Woy, Bauhinia race
Mi
Published on 25t
60 Screening of (Caesalpiniaathogens
H.Tambekar2
@gmail.com ogy, Sanjivani Coarch, Kopargaon
ge Baba Amravat
e plant leaves ds, and tannins.d methanol extrarial activity were
pathogens sucia, Enterobacteronella typhi, Sts had wide rang petroleum ethe
acterial activity aves of Bauhiniarug from natura
ords emosa, enteric ba
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Solvent Extraceae) against
2, and S. B. Ka
ollege of Pharman ti University, Am
reveals the pr. Petroleum ethacts of leaves oe studied by disch as Escheri
r aerogenes, Psetaphylococcus ege of antibacterer extract, wher
than chlorofora racemosa was
al origin for pre
acterial pathogen
Conference on
1st Century February 2011 tract Book
Page 70
racts of t Enteric
asture1
aceutical
mravati
resence of her extract, f Bauhinia c diffusion ichia coli, eudomonas epidermidis ial activity re as ethyl rm extract. s carried in evention of
ns
Progressiv
Modeand CDepa
Viral D
1 Department2 Departm
Banaplant has an hbanana contailack of viable is vulnerable tproduction. Bin India (Joshplant virus wiused to detectprimer specifiResearch, Batemperature, famplification successfully owhich did notused to screen
RT
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Diagnosis by U
S. O
t of Biotechnologment of Biotechno
ana is an importaherbaceous stem n large, hard seeseeds, propagat
to many diseasesanana Chlorosisi and Joshi, 197th a tripartite get the presence oic to coat proteinangalore. Diffeforward and reve
protocol. The obtained. The ret show any amp
n a number of co
- PCR, Banana
Arts, Science 11005
biology
PP6Using RT – P
Chlorosis
O. Gupta1 and
gy, Modern Collology, H.P.T. Ar
ant fruit crop of tand belongs to teds but the domtion is done veges amongst which
s disease was firs4). The causativ
enome. In the cuof the virus fromn gene were procerent parametererse primer conccDNA was am
esults were complification with rms prior to plan
KeChlorosis Disea
Mi
Published on 25t
61 PCR: A Case
Disease
B. A. Aglave2
lege of Arts, Scierts and R.Y.K. Sc
tropical and sub-the family Musa
mesticated varietietatively, by meh viral diseases ast reported in Au
ve organism, BSurrent investigatim the infected pcured from Indiars such as ancentration etc wemplified and th
mpared with thatthe primers. Th
ntation, thus avo
y Words se, Musaceae, R
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Study on Ba
2*
ence and Commecience College, N
-tropical countriaceae. The wild res are seedless. ans of corms. Bare the major conustralia (Magee,V is a positive son RT-PCR tech
plant samples. Tan Institute of Honnealing time, ere used to stand
he product of 7t of healthy planhe current protooiding heavy loss
RNA virus, CMV
Conference on
1st Century February 2011 tract Book
Page 71
nana
erce, Pune Nashik
ies. Banana relatives of Due to the anana crop nstraints in 1940) and sense RNA hnique was
Two sets of orticultural
annealing dardize the 700bp was nt samples col can be ses.
V
Progressiv
Modeand CDepa
S. M. KulkaS.R., S
Department O
Evenused as a rawtemperature anto extensive microbiology project, an atteBasidiomycetebiopulp from and 7 isolates bagasse and sbiopulp since content produclignin contenfermentation substrates. Thinoculated in After 30 daysActinomyceteproduced was making paper ecofriendly papowder. The strength and oPolysaccharid
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Biopulp
arni, Navale SShirke M. D., K
Of Microbiology
n in the world ofw material in thnd pressure. Thidestruction of techniques mayempt is made to es, Bacteria anlignocellulosic of Bacteria fromoybean industrythey are easily aces pitches in th
nt of the raw was performed
he individual typthese substrates incubation, lign
es showed decreathen used to mahelps in specific
aper; we have aecofriendly pap
odorless. Furtherde and Aspargina
Arts, Science 11005
biology
PP6p - An Eco-Fr
S. K., SulkshanKokani N. F.,
y, H. P. T Arts an
f paperless offiche conventionalis process requir
plants, there y be explored to
prepare the ecod Actinomycetematerial, we ham termite guts. Ay waste were theavailable and coe final product imaterials usedto get maximu
pes of Actinomto carry out sol
nin content was ase in lignin conake eco-friendly c texture and streadded binding aper made using r the isolates of Aase.
Mi
Published on 25t
62 riendly Appr
ne H. C., SharBhavsar S. P
nd R.Y.K Scienc
ce, we are forced pulping procesres many chemias been envirohelp in avoiding-friendly pulp. Bes has been repave screened 14 Agricultural byp
e two substratesst effective. The.e. paper. Thus i
d for preparatioum reduction o
mycetes, Bacterialid state fermentdetermined by ktent upto 0.1% apaper. The additength of the finagents’ viz. Pullupullulan & gela
Actinomycetes c
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
roach
rma L. P., Kha., and Joshi S
e College, Nash
d to use papers.ss by cooking uicals and high enonmental imbalg the deforestratBiodegradation oported. Hence tisolates of Acti
products such asused for the pro
e presence of ligit is necessary toon of paper. Sof lignin contena and also constation at room tekappa number. Tand 0.356%.The tion of binding aal product. Whileulan, gelatin & atin gave paper can be exploited
Conference on
1st Century February 2011 tract Book
Page 72
andelwal S. A.
ik 422005
Plants are under high nergy. Due lance. The tion. In our of lignin by to produce inomycetes s sugarcane oduction of nin in high reduce the Solid state nt in these sortia were emperature. Two of the biopulp so
agent while e preparing Fenugreek with good to produce
Progressiv
Modeand CDepa
Plasmid PClov
Te
Modern C
Pseudnosocomial inPseudomonas factors and Rantibiotics duepumps and prResistance is (plasmid-encoclove oil agaidone. Garlic Gram-positiveantagonist effGarlic Extractby disc diffusextract and 8-isolates was dnot detected in
Pseudomo
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Profiling ande Oil on Clin
ejlaxmi Inam
College of Arts,
domonas aeruginfection. It exhi aeruginosa ma
Resistance Transe to low permearoduction of plaeither innate re
oded). Antibacteinst multidrug reis reported to ee and Gram-nefect against Gramt and Clove Oil wsion method. Inh-33 mm by clovedone by the midn the isolates tes
onas aeruginosa
Arts, Science 11005
biology
PP6
d Antibacterianical Isolates
dar, Milky Sa
Science and Com
inosa is an opporibits an innate raintains antibiotfer Factors. Thebility to outer m
asmid encoded eesistance (chromerial susceptibilitesistant clinical exhibit broad spegative bacteria.m-negative bactewere used to chehibition zones re oil were obserdi-prep and Kadted so far.
Key Wo
, Aqueous Garli
Mi
Published on 25t
63
al Activity ofof Pseudomo
ahukar and Ka
mmerce, Ganesh
rtunistic pathogeresistance to a tic resistance ple bacterium is n
membrane, constiextended-spectru
mosomally-encodty testing of aquisolates of Pseu
pectrum antibact. Clove oil is eria. Different ceck antibacterialranging from 8-rved. In additiono and Liu meth
ords c Extract, Clove
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
f Garlic Extraonas aerugino
avita Parekh
hkhind, Pune- 41
en, and a commowide range of lasmids, both R
naturally resistanitutive expressioum β-lactamasesded) or acquiredueous extract of udomonas aerugterial activity ag
also known toconcentrations ol activity on fifte-25 mm by aquen plasmid profilinhod. However pl
e oil, Plasmid pro
Conference on
1st Century February 2011 tract Book
Page 73
act and osa
11053.
on cause of antibiotics. Resistance-nt to many on of efflux s (ESBLs).
d resistance f garlic and ginosa was gainst both o have an of Aqueous een isolates eous garlic ng of eight lasmid was
ofiling.
Progressiv
Modeand CDepa
Screenin
1D2Head
Lignispecifically wto fragment itlignin is carriperoxidase anforms family fungi for prodoxidize a varie
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ng of Fungal
Udata M. A.*
Department of Mid, Department of
in is an insolubwhite rot fungi det & diffuse to thed out by lignol
nd lignin peroxiof isoenzymes.
duction and assaety of phenols an
Lignolytic enz
Arts, Science 11005
biology
OP0Isolates for L
Peroxi
*, Joshi A. J.1
Microbiology, Dayf Microbiology, D
ble, high molecegrades it extrache hyphae & crolytic enzyme syidase. Mangane The aim of pr
ay of manganesend dyes.
Key Wozymes, Mangane
Mi
Published on 25t
04 Lignolytic Enides
1, and Ranjek
yanand Science Dayanand Scien
cular weight pocellularly. The fuoss the cell mem
ystem which incse peroxidase iresent work is se peroxidase usi
ords ese peroxidase, W
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
nzyme, Mang
kar M. K..2
College, Latur. nce College, Latu
olymer. So, varungi attack the lmbrane. Biodegrludes laccases, ms heme peroxidscreening of theing the property
Wood bark.
Conference on
1st Century February 2011 tract Book
Page 74
ganese
ur.
rious fungi ignin so as radation of manganese
dase and it e lignolytic
that it can
Progressiv
Modeand CDepa
Screenings
D
Lipasmicroorganism3.1.1.1). The these lipases.important nosvirulence factAcinetobacterRhodamine Bisolates were esterase positperformed. OnB method theproduced by taccount of theB was sensitiinclude purifiproperties of A
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
g of Lipases aspecies Isolate
Urvi
Department of Mi
ses (triacylglycems either alone opresent study a The organism
socomial pathogtors it possessesr spp. for prod, Phenol red andshown positive
tive. For confirmn phenol red agae same 8 isolatethese isolates weese methods showve and efficient
fication, molecuAcinetobacter lip
Acinetobacter, L
Arts, Science 11005
biology
PP6as Potential Ved from Diffe
Doshi & Kari
karishma@unicrobiology, Uni
erol acylhydrolaor together with ims at understan
m chosen for thgen which has n. In the present
duction of lipasd Tributyrin Agar
for lipase prodmation, Phenol ar 8 isolates weres were screeneere further quantwed that Rhodamt in screening thlar characterizapases.
Key WoLipases, Virulen
Mi
Published on 25t
64 Virulence Facerent Hospita
ishma Pardes
nipune.ac.in iversity of Pune,
ases; EC 3.1.1.3)esterases (carbo
nding the possibhe study is Acinot been studiedstudy, we scree
ses by three dr Plate assay. By
duction some of red assay and
re shown to proded positive for ltified by titrimetrmine B plate asshe lipase producation & studyin
ords
nce, Rhodamine B
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ctor in Acinetals in India
si*
Pune 411 007
) are produced oxylic ester hydrble virulence prinetobacter, knod much for the ened 42 clinical different plate ay Tributyrin Agawhich also couRhodamine B
duce lipase. By Rlipase productioric method. A co
say at 0.01% of Rcers. Future studng the potential
B, Titrimetry
Conference on
1st Century February 2011 tract Book
Page 75
tobacter
by various rolases; EC roperties of own as an
variety of isolates of
assays viz. ar assay, 15 uld be only
assay was Rhodamine on. Lipases omparative Rhodamine dies would l virulence
Progressiv
Modeand CDepa
In Vitro St
Vais
Natur
being used sidistinct from oinexpensive, Umedicines. Thliterature survprovide a scieresistant pathopathogens likeEscherichia cdiscover newmechanism opossessing anestimating theeither crude eethanol, and mwell diffusionPsoralea coryjatamansi, SmGymnema sylmethanol extractivity againwas observedformulating thinfections and
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
tudies of Ant
shali Prabhun
Abeda I
re has blessed Iince times immother fields of mUnani medicineshe prime reasonvey is either in Pentific base and ogens like MRSe Staphylococcusoli are responsib
w antimicrobial f action. Unanintimicrobial proe antimicrobial aextraction or Somethanol are emn assay. Screeninylifolia, Swertia milax glabra, Wrilvestre against Gracts of Psoralst Candida albid with Pseudohe extracts into
d prove to be a bo
Unani System
Arts, Science 11005
biology
PP6timicrobial Pr
Medicinal
ne, Namra Gh
Inamdar Senior C
India with immememorial to curemedicine as the ds provide an eff
n for these mediPersian, Urdu or documentation f
SA, skin infectios aureus, Candidble for severe skcompounds witi plants producioperties can be ctivity of these poxhlet apparatus
mployed for this png was carried o
chirata, Embelightia tinctoria,
Gram positive, Glea corylifolia acans and Staphyomonas aerugimedicines Una
oon for the socie
Key Wom, Drug resistan
Mi
Published on 25t
65 roperties of El Plants
ansar and Su
College, Camp, P
ense variety of me diseases. Unadrugs it uses are fective and safe icines not being Arabic language
for this system. ons are becominda albicans, Psekin infections. Tth diverse cheming wide range
harnessed to tplants, active pris. Different solvpurpose. The extut using methanlia ribes, RubiaPicrorhiza kurr
Gram negative aand of Embeliaylococcus aureunosa and Esc
ani system can pety.
ords nce, Antimicrobia
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Extracts from
upriya Bhopal
Pune
medicinal plantsani system of m
natural. Being nalternative to thwidely known
e. Hence there iDue to emergen
ng very commoneudomonas aerugThere is a growimical structures
of secondary mtreat skin pathoinciples are extravents such as ctracts are then su
nol extracts from a cordifolia, Naroa, Cyperus rotand fungal pathoa ribes showed us while minimucherichia coli. provide a cure t
al activity
Conference on
1st Century February 2011 tract Book
Page 76
m Unani
le
s which are medicine is natural and he existing is that the s a need to
nce of drug n. The skin ginosa and ing need to and novel
metabolites ogens. For acted using chloroform, ubjected to
m plants like ardostachys tundus, and ogens. The maximum
um activity Thus, by
to the skin
Progressiv
Modeand CDepa
Lignin Deg
V. A
Depar Ligni
due to its inproperties, tencolored effluehazardous. Milignin and a wniche eight baout of which tof lignin. Crudof methylene mill effluent g50% to 58% peroxidases frpaper-pulp mibased effluentefficiently usindustries.
Lignin perox
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
grading PeroxTe
A. Tile *: Bor
rtment of Microb
in, the nature’s pntense unaesthends to be a recents due to presicrobial extracelwide range of c
acterial and two athree bacterial ande extracts of ligblue dye during
gave 60% to 75%decolourization
from mixed consill effluent and t which is signi
sed in biobleac
xidase, Biobleach
Arts, Science 11005
biology
PP6xidase in Bioxtile Dye-Bas
khataria B. V
vaishali_tile@y
biology, K.T.H.M
plastic is the majetic brown coloalcitrant composence of large allular lignin perocomplex aromatactinomycetes isnd one actinomygnin peroxidase enzyme assay.
% color reductionwas observed.
sortia gave 80%70% to 75% deificantly high. T
ching and biod
Key wohing, Paper-Pulp
Mi
Published on 25t
66 bleaching of sed Effluents
V, Bhalerao V.
yahoo.co.in M. College, Nash
ajor pollutant froor, hydrophobicound. Textile dyamount of mixtuoxidase enzyme ic dyestuffs. Fro
solates were screycetes were ableenzyme gave 60The studies on bn and in case ofThe heterogene
% to 85% color ecolourization inThis system of
degradation of
ords: p mill effluent, T
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Paper-Pulp s
B, Aher N. S.
ik, Pune Univers
om paper-pulp mcity and poor mye-based industrure of dyes whhas a potential
om various enveened for lignoly to decolorize 440% to 76% decobiobleaching of f textile dye- baseous combination
reduction in trn treatment of tlignin peroxidaeffluents from
Textile dye-based
Conference on
1st Century February 2011 tract Book
Page 77
Mill and
.
sity
mill effluent mechanical ries release hich is also
to degrade vironmental ytic activity 4% to 49%
olourization paper-pulp
sed effluent n of lignin eatment of textile dye-ase may be
respective
d effluent
Progressiv
Modeand CDepa
Bacteria
V. A
Depar
The dis an importannanoparticles to obtain becacontrolled reasynthesis but great concern stabilized diresuch as bactemetals througinteresting as bacteria is invsilver resistantextracellular, t24 hours. AgNvessels that conanoparticles and UV-visibEscherichia crange of 12–2effect is dose Gram-positivepathogens of clinical isolatAspergillus fuanatomy and considerable applications.Inefficient remoand removal o
Silver-Nan
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
al Synthesis o
. Tile*., S. P.
tment of microbi
development of nt aspect of nanare available, buause it depends action environmthese methods ufor environmen
ectly in the proceria, yeast, and gh reduction of tnanofactories. In
vestigated by bot organisms werthe test strains wNO3 at concentrontained the supethus produced le spectroscopy
coli and Bacillu25 nm with incr
dependent and e organisms.
the skin weretes and ATCC umigatous The rspore germinatiantifungal ac
n the area of watoval of pollutantof chemical and b
notechnology, Ba
Arts, Science 11005
biology
PP6of Silver Nan
Pawar., W. R
vaishalitile@yiology, K. T. H. M
reliable processe
notechnology todut a narrow and on the adjusted
ment. Colloidal muse toxic chemicntal reasons. Theess by proteins.Afungi play an imthe metal ions, n this study, the th intracellularly
re grown in convwere cultivated iration of 10mMernatants of Eschwere characteri. The silver nan
us subtilis.The preased stability ais more pronouAntifungal eff
investigated. Nstrains of Tric
results showed non. Thus, the st
ctivity, deserviter purification, ns and germs. Thbiological substa
Key Woacteria-Nanopart
Antimicr
Mi
Published on 25t
67 oparticles an
R. Sayyed., an
yahoo.co.in M. College, Nas
es for the syntheday. Many synthcontrolled size p
d concentration metal particles cacals in the synth nanoparticles syAlthough it is knmportant role inonly recently thproduction of siy and extracelluventional media cin their special c
M is separately aherichia coli, , Bized by transmisnoparticles wereprepared silver and enhanced an
unced against Grfects of silver Nano-Ag showechophyton rubrunano-Ag exertedtudy indicates thng further innanotechnology
he nanoparticles ances.
ords ticles, Extracellurobial
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
nd Its Applica
nd A.V. Bhosa
sik, Pune Univer
esis of silver nanhetic procedurespreparation seemof reacting chem
an be obtained bhesis protocol, wynthesized biolonown that micron the remediatiohis approach is lver nanoparticle
ularly. For intraccontaining AgNconventional conadded to the eacBacillus subtilis,.ssion electron m
e effectively pronanoparticles wnti-bacterial potram-negative bananoparticles
ed potent activium , Candida d activity on thhat the nano-Agnvestigation fo
offers the possibmay be used fo
ular, Intracellular
Conference on
1st Century February 2011 tract Book
Page 78
ations
le
rsity
nomaterials s for silver ms difficult micals and y chemical
which raises ogically are oorganisms on of toxic considered es by some
cellular, the O3, and for
nditions for ch reaction . The silver microscopy oduced, by, were in the tency. This acteria than on certain ity against albicans ,
he mycelial g may have or clinical bility of an
or detection
r, TEM,
Progressiv
Modeand CDepa
Study of Gr
Departmen
ESBLstudies have rproducing bacthe study wasCenter, Kadammultiple drug May 2009. A isolated from disc diffusionconfirmed by (42.13%) wersensitivity to Jarlier DDST were DDST psensitivity tesPiperacillin-ta(39.75%) & KMajority of thfollowed by su
Extended Sp
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
f Extended Spram Negative
V.S.Vat
nt of Microbiolog
L producing Grareported on the pcteria may have es carried out at Dmwadi, Kolhapuresistance in famtotal number ofvarious clinical
n test as per CLusing DDST (Dre potential ESthird generationfor these 83 iso
positive. All thst (AST). All thazobactum (87.5%Klebsiella pneumhe ESBL produurgical ward 16
pectrum beta-La
Arts, Science 11005
biology
OP0pectrum Betae Bacilli in Fa
tkar, P. G. Sh
vsatish999@red
gy, Dr. D. Y. Pa
am Negative Bacprevalence of Eevolved in severDept of Microbi
ur, to examine themily Enterobactf 197 GNB belosamples & stud
LSI (Clinical LabDouble Disc SynSBL producers n Cephalosporinolates. Seventy
he ESBL produchese 70 isolates%) and Amikaci
moniae 16 (37.20ucers were isola(22.85%) and fr
Key Woctamases (ESBL
Mi
Published on 25t
09 a-Lactamase amily Enterob
adija, S. J. Gh
diffmail.com til Medical Colle
cilli is a worldwiESBL producers ral hospitals all oiology, D. Y. Pae incidence of Eteriaceae duringonging to familydied for ESBL prboratory Standaergy Test). Out on the basis os (3GC). ESBL (35.53%) isolatecers were testes were susceptibin (81.2%). Amo0%) were most ated from the mom ICU 12 (17.
ords L), 3GC, Resistan
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
(ESBL) Prodbacteriaceae.
hosh
ege, Kolhapur, 4
ide problem. Altin Indian hospit
over the countryatil Hospital and
ESBL producing g the period of Dy Enterobacteriaroduction by Ki
ards Institute) stof 197 isolates,
of resistance or production is d
es out of these d for MIC andble to Imipenemong these isolatecommon ESBL
medical ward 2014%).
nce, Enterobacte
Conference on
1st Century February 2011 tract Book
Page 79
ducing
416006.
though few tals, ESBL . Therefore d Research strains and
Dec 2007 to aceae were irby- Bauer tandards & 83 isolates decreased
detected by 83 isolates
d antibiotic m (100%), es E.coli 33
producers. 0 (28.57%)
eriaceae.
Progressiv
Modeand CDepa
AccumuPseud
Vid
PHA
organisms. Buhydrocarbon SStyrene is releprocessing indrespiratory tracytoplasm of nutrient enviroPseudomonas and aliphatic thermoplastic disposable iteantibiotic. Ourcause environand can be usand also envproducts. It is derived from pof the industri
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
ulation of Polydomonas spp
dya N. UdawaNew Arts
A are polyester ofut PseudomonasStyrene, which iseased in milliondustries. Styrenact,and nervousmicroorganism
onment such as spp. Provides aPHA accumulatpolyester. It i
eams,food packar routinely used
nmental hazards.ed as alternativevironmentaly haimportant for thpetroleum oil suies and society.
Arts, Science 11005
biology
PP6yhydroxyalkand Effect of
Accumu
ant, Smita A. Comm. and Sci.
f various hydroxs can produce s also much resis
ns of kilograms ine is a suspectes system. PHA
m and it acts as nitrogen deficiena new link betwtion. PHA posses used in the aging, biodegradpetroleum based
. But the PHA ae to conventionaazardous styren
he global commuuch as plastic. PH
Mi
Published on 25t
68 kanoate (PHAf N2 Concent
ulation
Daule and D. College, Ahmed
xyalkanoate whicPHA efficientlystant to microbiain a year from pd carcinogen,teraccumulated asstorage source
ncy. The converween an aromatiess properties siproduction of pdable rubber,in d plastics are noare the bioplastil plastic for the
ne is convertedunity to have an HAs at least will
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
A) from Styreraction on PH
. D. Giramkardnagar
ch are synthesizey by using toxial degradation. petrochemical anratogen ,toxic ts discrete granu
of carbon in ursion of styrene ic environmentaimilar to variouplastic utensils cosmetic,medicn biodegradableics which is biobetterment of en
d into ecofrienalternative for thl be a solution fo
Conference on
1st Century February 2011 tract Book
Page 80
ene by HA
r
ed by many c aromatic
nd polymer to kidneys, ules in the unbalanced to PHA by
al pollutant us synthetic
and other ine and as
e and hence degradable nvironment dly useful he products or the most
Progressiv
Modeand CDepa
Biosurf
Vijendra
1D2Institute o
A main PG mediumand was partpreparative thpowder made lowered the smicelle concekerosene-wateagainst Staphyalso able to bioremediationkilling of HeLaeruginosa 0biosurfactant anumber of environmental
Biosurfac
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
factant Produ
A. Kavatalka
Department of Mof Bioinformatics
arine isolate of Pm at pH 7.2, 30oCtially purified
hin layer chromup of lipid and
surface tension entration of 25er interface. Partylococcus aureu
chelate chromn. The crude biLa cells. The pa1 and 74% Acalso had an intebeneficial traitl and biomedical
ctant, Pseudomo
Arts, Science 11005
biology
OP0uction by a M
r1, Smita S. BBalu A. Cho
directoribb@un
Microbiology, Uns and Biotechno
Pseudomonas aeC at 120 hours. Tby extraction w
matography. Thed polysaccharide(SFT) of distill5µg/L and redtially purified bious, Bacillus subtmate and ferrouiosurfactant at cartially purified bcinetobacter baunse antioxidant ts make this l applications.
Key Wonas aeruginosa,Antioxidant, Me
Mi
Published on 25t
07 Marine Strain
Bhuyan1, Peehopade1,2*
nipune.ac.in iversity of Pune,logy, University
eruginosa produThe biosurfactanwith chloroform biosurfactant ie with a yield oed water to 49.
duced interfaciaosurfactant exhibtilis, Bacillus puus ions indicat
concentration 50biosurfactant dis
umannii biofilmactivity of 83.55
biosurfactant
ords SFT, IFT, Antimetal chelation
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
n of Pseudom
hu S. Pardesh
, Pune 411 007 of Pune, Pune 4
uced a potent biont is a secondary m: methanol fois a brown, watof 1g/lit. The bio.377 mN/m, had
al tension (IFTbited antimicrobumilus and E. cting its potenti
00 µg/ml exhibitsrupted 97% Pses. Additionally, 5 % per 20 minuexcellent cand
microbial, Antic
Conference on
1st Century February 2011 tract Book
Page 81
monas
hi1 and
411 007
osurfactant metabolite
ollowed by ter soluble osurfactant d a critical
T) between bial activity coli. It was ial use in ted 83.9 % eudomonas the crude utes. Large didate for
cancer,
Progressiv
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ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
tilization of M
katesh. M. SaShilpa. S.
ment of Microbio
nomycetes were. Total nineteen
production. Bolasses as the soreptomyces S2. Ece on biosurfactaNaNO3 (1g%)urification of bio
Biosurfactant, Bi
Arts, Science 11005
biology
PP6Molasses for B
by Streptom
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smitabhuyan@
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Key wo
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Mi
Published on 25t
69 Biosurfactantmyces S2
li S. Mane., Gand Smita. S. B
@gmail.com ollege of Arts, Scune 411 005
m soil and scrobtained, out
roduction was e. Maximum bio
onmental paramewere checked. pHon resulted in der process.
ords Actinomycetes,
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
t Production
auri M. PawaBhuyan*
cience & Comm
reened for prodof which, fivecarried out in
osurfactant prodeters such as pHH of 7.5 , Pb (0
maximum bio
Streptomyces
Conference on
1st Century February 2011 tract Book
Page 82
ar,
merce,
duction of e exhibited n medium
duction was H, salt and 0.1g%), Mg osurfactant
Progressiv
Modeand CDepa
Isolation
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Indus
are dischargedTwo isolates wsupplemented guilliermondiiof phenol andmetals such aspotential in biheavy metals Plasmid was electrophoresilaboratory as to obtain highdegrade phenophenol concennitrogen sourcconsortia in im
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
n, Characteriz
Uzma T
epartment of Mic
strial wastewated into the naturawere obtained bwith phenol (1
i were selected od degradation es Ni+2 Cu+2, Pb+2
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isolated by is. Horizontal gwell as simulate
h degradation efol at different pHntration. Efficience (20 mg of N2/mmobilized form
Arts, Science 11005
biology
PP7zation and A
T. Momin and
crobiology, The
rs containing hial ecosystem repby selective enri00- 1000 µg/mlon the basis of grefficiency. Both2 and Cd+2 as welasmidial locatiowas detected in oalkaline lysis
gene transfer bed conditions. Thfficiency. The coH, temperature, nt biodegradation/mg of phenol) u
m also degraded p
Mi
Published on 25t
70 Application of
Varsha K. Va
institute of Scien
igh concentrationpresenting seriouichment techniqu). Both the isolarowth response t
h the strains shell as to various on of genes confeonly one isolate method and dy transformatiohe two isolates wonsortia were evorganic and inorn was observed a
urea, phenol concphenol.
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
f Phenol Degr
aidya
nce, Mumbai 32
ns of phenolic cus environmentaue using minimates, identified ato increasing conowed resistanceantibiotics indic
ferring resistanceby acridine oran
detected by agon was carried were used as a c
valuated for theirganic nitrogen at pH 7, temperacentration 1000 µ
Conference on
1st Century February 2011 tract Book
Page 83
raders
compounds al problem.
mal medium as Candida ncentration e to heavy cating their e to phenol, nge curing. garose gel out under
consortium ir ability to source and ature 30°C, µg/ml. The
Progressiv
Modeand CDepa
Antibacte
Prati
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Media new anti-isanctum), gsativum), gantibacterial ain vitro antibacetone werehigh antibacte
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
erial Activity
ibha A Patil ,
artment of Micro
icinal plants areinfectious agentginger(Zingiber guava(Psidium activity .Out of tbacterial suscee used for extrerial activity aga
Solvent extrac
Arts, Science 11005
biology
PP7
of MedicinaPathog
, Shilpa S Muj
obiology, Moder
e important for ts. Plants like
officinale), lemguajava), le
these plants neeeptibility tests. raction .The ethainst Bacillus, S.
Key wocts, Medicinal pl
Mi
Published on 25t
71
l Plants againgens
jumdar, Madh
rn College, Shiva
the study of t Neem (Azard
mon(Citrus auremon grass(Cymem ,tulsi , ginge Solvents like
hanolic and ac aureus and Kle
ords lants, Antibacter
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
nst Gastroint
huri Amrutka
ajinagar, Pune 0
traditional uses dica indica ), turantifolia), gambopogon citraer ,lemon weree methanol, ethcetone extracts ebsiella.
rial activity
Conference on
1st Century February 2011 tract Book
Page 84
testinal
ar.
05
and act as ulsi(Ocium
arlic(Allium ate) show taken for hanol and exhibited
Progressiv
Modeand CDepa
Utilizatio
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recognized aswhich affects soil by oil caupermeability acounter measueffective and mechanic worwere enrichedbacterial strain
Thesand biochemiOut of these 3Pseudomonasactivity had bsteel industry analyzed for Alkalinity, Chcomparison ofwhich are inibiodegrader.
Oil
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
on of PetroleuSoil and
Dalvi C. S
DNew Arts
ronmental pollus one of the momany species o
uses it to lose itsand binding capure to remediateefficient strategy
rkshops were seld using Bushnens capable of usie isolates were ical characteristi30 isolates, 7 sh, Serratia, Bacieen observed oneffluent from Aphysico-chemic
hloride and Phosf oil degrading citially present i
degradation, Lip
Arts, Science 11005
biology
PP7
um HydrocarContaminate
S., Satbhai M
Department of Ms, Commerce a
Ahmedn
ution with petrost serious problef plants and anim
s useful propertiepacity. Hence, the soil contaminay to speed up thlected where theell-Hass enrichming used engine identified on theics using Bergyhowed prominenillus, Micrococcn Egg yolk agar,Ahrnednagar MIcal characteristicsphate. Along wicapability of the in the effluent
Keywo
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Mi
Published on 25t
72
rbons by Baced With Engi
M. A., Bhutada
Microbiology, and Science Conagar
oleum and petrems. Oil is relemals as well as es such as fertilihe attempt of stuated with oil. Bhe cleanup procee soil had been cment medium fooil as a sole carbe basis of standa’s manual of Det oil degrading a
cus, Proteus and Phenol red agarIDC. (Indian Secs which includith these the furtisolates obtainedsample and pre
ords hvsico-Chemical
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
cteria Isolatedine Oil
a S. A.,
ollege,
oleum productsased into the enhumans. Contam
ity, water holdinudy was to inve
Bioremediation pess. For that purcontaminated. Soor a week. A tbon source were ard morphologiceterminative Baactivity. These id Alcaligens. Tr and oil powdereamless Metal Tde BOD, COD,ther study also ind from soil and teparation of car
characterization
Conference on
1st Century February 2011 tract Book
Page 85
d from
s has been nvironment mination of ng capacity, estigate the provides an rpose three oil samples total of 30 isolated.
cal, cultural acteriology. isolates are
Their lipase r agar. The
Tubes) was , DO, pH, ncludes the the bacteria rrier based
n
Progressiv
Modeand CDepa
Biodegrad
B. A. K
Insec
compounds anderived. The microorganismdegraded are persistent. Usethe entry of thsuch pesticidefrom such instheir eliminatconstructive abiodegradationpest controllerleaf-feeding pused for screesubjected to aliquid media awas investigaisolates. A comfurther intendelimination ofdegradation st
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
dation of Com
Karode, N. B.
DPSGVPM
cticide degradatind, in some cas
most commonms, especially t
called non-perse of persistent inhese insecticide
es is a major envsecticides so thetion or minimi
approach focusedn of a pesticide trs commonly us
pests on cotton, sning of organisma range of concand the colony fated to determinmparative accou
d to determine thf the supplied qtudies in the field
Arts, Science 11005
biology
PP7
mmercially U
Chaudhari, SJobanp
arpana_jl2@redDepartment of MM’s, ASC Colleg
ion is the breakises, down to then type of degrthe fungi and sistent while thnsecticides like s in the ecosyst
vironmental concre is a serious nization in the d towards envirothiamethoxam aned nowadays un
soybean etc. Serims which could dcentrations of peforming units anne the concentr
unt of the isolateshe number of daquantity of the d.
Mi
Published on 25t
73
Used Insectic
. S. Patil, P. Tputra
diffmai1.com Microbiology, ge, Shahada, 425
ing down of toxe original elemeradation is carbacteria. Insect
hose which resisMalathion, Carbtems. Contaminacern. There is a need to develop
environment. Tonmental managnd diafenthiruon
nder various tradial dilution and pdegrade these peesticides from 1
nd absorbance inrations which cs has been depicays which wouldpesticides and
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
ides by Soil I
T. Patil, and A
5409
xic pesticides intnts from which rried out in thticides which ast degradation abofuran etc, has ation of soil andpotential carcinoremediation pro
The present argement. It highlign. They are broade names for despour plate technesticides. The iso10-100 mgL’ inn liquid media recould be toleratted in the presend be required fouse the data fo
Conference on
1st Century February 2011 tract Book
Page 86
Isolates
A. H.
to nontoxic they were
he soil by are rapidly are termed resulted in d water by ogenic risk ocesses for rticle is a ghts on the d spectrum struction of niques were olates were n solid and espectively ted by the ntation. We or complete or trails on
Progressiv
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Due t
situ biodegrainevitably hasmeasured in microbial popdirect measurdegradation pamounts of thsuch methods means have fdiesel oil was isolates of soipolluted with with the unpodegradation bythus determinefrom the thirddegradation w
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
Biodegrada
V. R. Ko
of Microbiology,
to wide spread udation of pollus hazardous effe
laboratory testpulations, gravimre of bioremediaprocess aids the he oil can be clea
find limited usefound considerabmonitored over il identified as Pthe oil samples
olluted control say these isolates ed. There was ad day continuin
was investigated t
Diese
Arts, Science 11005
biology
PP7
ation of Diese
othari’ and A.
arpana_j12@red, PSGVPM’s, AS
Maharashtr
use of diesel oil,utants, has receects on human ts of bioremed
metrically measuation efficacy is
elimination of ared by various e under natural eble significance a twenty-seven Pseudomonas aeat a loading rateamples were seeat the interval o
a consistent decrng till the twento be approxima
Key Woel oil, Gravimetri
Mi
Published on 25t
74
el Oil by Soil
. H. Jobanput
diffmail.com SC College, Shahra, India
, the natural atteneived a large alife and safety.
diation efficacy uring the quantitys the monitorinspilled oil from
physical and chenvironmental cin the past few
day period usingeruginosa and P
e of 2% (v/w). Theded with the twof three days, upease in the quan
nty-seventh day. ately 54 % under
ords ic, Bioremediati
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
l Isolates
tra2*
hada, 425409, N
nuation, which ramount of atten The parameter
include enumy of residual oil
ng of diesel oil. m the environmemical methods
conditions. Microw decades. Degrg gravimetric mePseudomonas sphese soil sample
wo isolates. The p to twenty sevenntity of residual
The approximar un-optimized co
on
Conference on
1st Century February 2011 tract Book
Page 87
Nandurbar,
relies on in ntion. This rs typically
meration of . The most Microbial
ment. Large . However, obiological radation of ethod .Two
pecies were es, together
rate of oil n days was oil starting ate rate of onditions.
Progressiv
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Raw w
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Wastcountries. Esbecoming anfilling, land directly into bread edgeshospitality aproper and scsource of migrowth medbiofungicide like biosolidTreatment Pdays. Waste were collecteon sterilized hrs, and obssporulation sTrichodermabiobeneficiatmethods for is most prom
Tri
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
wastes as altes
ushikesh Sank
partment of Mic
te disposal isspecially bioson environmenta
spreading, anwaste dumps f, and bread c
and hotel induscientific manneicrobial nutrienia for Trichod and promotesds were colle
Plant, Erandwapotatoes were
ed from restau raw wastes aserved for grosubstantiating a viride. Usintion of wastesmass productio
mising for susta
ichoderma viri
Arts, Science 11005
biology
PP7
ernative medustainable de
kpal*, Sunil D
rushisankpal@crobiology, Fer
s of major colids generated
al nuisance. Cod incineration.from vegetablecrumbs are distry. All theseer. The comfornts. Hence, in tderma viride. s good plant grcted from Pu
ane, Pune. Thee collected fromurants. Spores septically, incuowth. The resthat these was
ng this simpls. Moreover, ton of Trichodeinable develop
Key wo
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Mi
Published on 25t
75
dia for Trichoevelopment
Deshmukh an
@gmail.com rgusson Colleg
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rting fact is thatthis study we uTrichoderma vrowth in many
une Municipal ese biosolids wm vegetable mof Trichodermubated at roomults showed vstes can be vele know-how this technique
erma viride. Biment.
ords
osolids, bread e
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
oderma: towa
nd Vinay Rale
ge, Pune 41100
veloped and dater treatment hey are disposeted potatoes aragricultural landn large quantito be disposedt these wastes aused these raw viride is a we
y ways. Waste Corporation’s
were sun-driedmarket while brma viride were m temperature very good groery good medi
we can brie is cheaper thobeneficiation
edges, potatoes
Conference on
1st Century February 2011 tract Book
Page 88
rds
04
developing plants are
ed by land re thrown ds. Bread, ities from d off in a are a good wastes as
ell known materials
s Sewage d for four ead edges sprinkled for 48-72
owth with ia for the ng about han other of wastes
Progressiv
Modeand CDepa
Char
*Departmen
PashRamnadi rivudrinking purpdrinking purbecause of hindustrial wecosystem isPashan lake wand to study of any aquatwill pave a wused again as
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
racterization
Rahul N
nt of Microbiol
han lake is a ulet of Mutha pose. At presenrpose because
human activitiewaste water frs under stress owater with respits microbial d
ic system and way to know ws a potable wat
Arts, Science 11005
biology
PP7
n and biodive
Nimbalkar and
ogy, Sinhgad CPune-
fresh water
river. In the pnt this water is of deteriorate
s like washingfrom nearby hof water pollutipect to its physdiversity. Physits biodiversitywhether in fututer resource or n
Mi
Published on 25t
76
srsity of Pash
d Varsha Kulk
College of Scien4 1
man made repast, water from
not used by Md water qualit of trucks and human settlemion. Present stusico-chemical asico-chemical ay are influenceure this fresh wnot?
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
han lake wat
karni
nce, Ambegaon
eservoir constrm this lake was
Municipal Corpoty. This deteridumping of se
ments. At preudy is to characand biological pand biological pd by each othewater resource
Conference on
1st Century February 2011 tract Book
Page 89
er
n Budruk,
ructed on s used for oration for ioration is ewage and esent this cterize the properties properties
er. Results e could be
Progressiv
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types of m
circulation.
sterile plast
overnight, t
mannitol sa
the basis
characteristi
found micro
species, kle
hemolytic s
ve Education society’s
ern College of ACommerce, Pune 4
artment of Microb
nfluence of
artment of Micr
present inve
microbial flora
Paper notes
tic bags, inoc
thereafter sub
alt agar, and m
of colony c
ics, Enzyme
oorganisms a
ebsiella spec
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Arts, Science 11005
biology
PP7f Microorga
Manjiri Mu
robiology, Prof. Science co
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a present on
were collecte
culated in pe
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mac-conkey ag
characteristics
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Mi
Published on 25t
77 anisms on I
ukund Deshpan
Ramakrishna M
ollege, Akurdi 44
as carried out
Indian curre
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eptone water
ifferent medi
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s, Gram’s s
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lla species,
ative staphylo
State Level
icrobiology in 2125th – 26th F
Abst
th February 2011
Indian Curr
de
More Arts, Comm4
t to observe
ency (paper n
rent places of
for 5 min, i
a such as blo
bated for 24 h
staining, Bio
ntify, Some co
cocci, E-coli,
α- hemolytic
ococci etc.
Conference on
1st Century February 2011 tract Book
Page 90
rency
merce and
different
notes) in
f Pune in
incubated
ood agar,
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ochemical
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Bacillus
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Author Index
Author Page No. Author Page No. A. H. Jobanputra 86,87 Deepali Giremar 64 A. J Joshi 74 Deepali Mane 21, 82 A. R. Desai 22 Dhutekar S.S 55 A.V. Pundle 39 Divya Nair 20 A.V.Bhosale 78 Diwan V.A 12, 30 Aasawari S. Pawar 01 G.K.Wagh 63
Abhay Raorane 02, 64 Gauri Devasthale 20 Abhijit More 09, 62 Gauri Jadhav 37 Aher N. S 77 Gauri Pawar 21, 82 Akshay Borkar 03 Ghule P.V 15 Alka Jagdale 04 Gore Suneeti 67 Alok Vyas 20 Hrishikesh D. Dhamal 05 Amar D. Jadhao 05 Hrishikesh Joshi 22 Amit Sinnarkar 63 Hussain Khokhawala 41 Anil M. Garode 06 Indulkar V.S. 23 Anita N. Katkade 11 Jadhav Sandip R 44 Anupama Sonawane 43 Jain Reena R 24 Anushka Devale 04 Jayesh A. Patil 25 Aparna Das 60 Jhilmil Ghoshal 20 Archana J. Marry 08 Jogi M.M. 25 Avinash D. Bholay 11, 13, 17 Joshi A. J 74 Avinash Sunder 39 Joshi S. A 72 Ayushi.A.Chaurasiya 07 Jyoti Mantri 08, 50 B. A. Karode 86 Kadam Shradhha D 24 B.A. Aglave 71 Kamble. A 19, 27 Balu A. Chopade 81 Kapadnis B.P 19, 27 Barbuddhe S.B. 02 Karishma R. Pardesi 40, 45, 75 Bedare Monika 10 Kaveri S. Palekar 11 Bedse,Tanuja D 44 Kavita Parekh 73 Bhadale S. V 15 Kawade S.S 30 Bhalerao M.N 15 Ketki Nalawade 28 Bhalerao V. B 77 Khadpekar A 29 Bhalkikar S.B 12 Khandelwal S.R 72 Bhavsar S. P 72 Kokani N. F. 72 Borkhataria B. V 77 Kulkarni 15 Borse Priti K 13 Kurup Sunila .B 17 Chavan K.J 16 Lalit Lande 31 D. D. Giramkar 80 Laxmi Nair 39 Dalvi, C. S 85 M. S. Vrushali 32
ii
De Souza N.A 19, 27 Madhuri A. Udata 74 Deep.V.Barot 18 Madhuri Amrutkar 10, 65, 84 Deepak R. Patil 34 Madhuri Londhe 33 Mahesh R. Ghule 34 Priyanka Mane 49 Maid Sneha A 13 Purnima Ranawat 50 Manasi Karmarkar 35 R.B.Vaidya 07, 66 Manoj Bhosale 03 Rahatekar Rohini .R 17 Meenakshi Dhar 36 Rahul Brid 51 Meghana Kukarni 35 Rahul Nimbalkar 89 Mehta Khyati 46 Rajendra Choure 01, 28, 42, 51 Milky Sahukar 73 Rajesh M. Chomal 34 Mokshada Godbole 38 Rakhi Tari 52 Monika More 37 Rakte Snehal B 13 More Bhagyashree V. 25 Ranjekar M.K 12, 16, 23, 30,
55, 74 N. B. Choudhari 86 Ratnakaran Neena 46 N.Rutika 32 Ravi V. Kale 26 Namra Ghansar 76 Ravindra Baviskar 53 Nasim Kherad 36 Rebecca.S.Thombre 29, 56, 68 Navale S. K 72 Reena A.Dighe 54 Neelima Kulkarni 38, 41 Repe A.B 55 Neha Naik 39 Riddhi J. Shah 40 Neha S. Vora 40 Roma A Chougale 57 Nikita L. Lad 42 Rushikesh Sankpal 88 Nilesh A. Sonune 63 Rutuja.R.Phatate 56 Nilesh P. Anandwani 25 S. A. Bhutada 14, 70, 85 Niruta Y. Killedar 43 S. B. Kasture 70 P. G. Shadija 79 S. J. Ghosh 79 P.S. Patil 44 S. M. Kulkarni 72 P.T. Patil 86 S.B.Dahikar 70 Pandurang Lahare 41 S.O. Gupta 71 Pankaj .S. Picha 45 S.P.Pawar 78 Parkar S.D 19, 27 Sabah Shaikh 58 Patil Jayesh A 25 Sagar Vandra 59 Patil N.N 15 Salokhe S.G 03 Patil S. S. 86 Samriddhi R. Sharma 60 Patil S.D 16 Sandeep Gajbhiye 61 Patil Sham A 44 Sangeeta Jangam 69 Peehu S. Pardeshi 81 Saral Ghosh 54 Poojari Pritika 46 Satbhai, M. A 85 Poorva Dabir 35 Sayali Mahajani 09, 62 Pradnya Kanekar 09, 62, 63 Seema S.Sarnaik 09, 62, 63
iii
Pradnya N. Nadivkar 47 Shahina Shaikh 64 Prajakta Bhagwat 48 Sharma L. P 72 Prakash R. Thorat 26 Sheetal .P. Pardeshi 45, 47 Pratibha A Patil 84 Shikha Malla 65 Priya Bendre 48 Shilpa Mujumdar 04, 21, 31, 33,
49, 53, 65, 82, 84
Sunny Gaikwad 53
Shilpa Sabnis 52, 58, 59 Supriya Bhopale 76 Shilpi.R.Chand 66 Supriya V. Suryawanshi 25 Shirish Shah 03 Tambekar D. H 14, 70 Shirke M. D 72 Tejlaxmi Inamdar 73 Shirode Abhinav 46 Udata M.A. 74 Shradha B. Nadhe 40 Urvi Doshi 75 Shradha Bashetti 04 Uzma T. Momin 83 Shreyas Kumbhare 41 V. R. Kothari 87 Shubhangi Puranik 68 V.A. Tile 77, 78 Siddiqui A.G 23 V.S.Vatkar 79 Singh Viveka 67 Vaidehi Dande 39 Smita A. Daule 80 Vaishali Prabhune 76 Smita S. Bhuyan 04, 21, 31, 33,
49, 53, 81, 82 Varsha Kulkarni 89
Snehal B. Bari 63 Varsha.Vaidya 18, 83 Sonal.P.Bathija 56 Vidhate Pushpa.S 17 Sonawane H.V 03 Vidya N. Udawant 80 Sourabh Mehta 68 Vijendra A. Kavatalkar 81 Srivastava Shreya 67 Vinay Rale 36, 88 Sulkshane H. C 72 Vivek N. Bobade 05 Sumedh S.Deshmukh 38, 43 Vrushali Patil 48
Sunakar 61 Vyankatesh M. Sanam 21, 82 Sunil Deshmukh 88 W.R.Sayyed 78 Sunil Tiwari 69 Yogesh S. Shouche 34
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