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Page 1: Abstract Book - Home - Modern college of Arts, …moderncollegepune.edu.in/wp-content/uploads/2014/12/...Abstract Book State level Conference on ‘Microbiology in 21st Century’
Page 2: Abstract Book - Home - Modern college of Arts, …moderncollegepune.edu.in/wp-content/uploads/2014/12/...Abstract Book State level Conference on ‘Microbiology in 21st Century’

Abstract Book

State level Conference on ‘Microbiology in 21st Century’

25th – 26th February, 2011 

Dr. Gajanan R. Ekbote Chief Organizer and

Chairman, Business Council, Progressive Education Society, Pune 5.

Dr. Rajendra. S. Zunjarrao Convener and

Principal, Modern College of Arts, Science and Commerce, Pune 5.

Dr. (Mrs.) Shilpa S. Mujumdar Organizing Secretary and Head, Department of Microbiology

Organized By

Department of Microbiology

Modern College of Arts, Science and Commerce Shivajinagar, Pune 5.

Under Quality Improvement Programme of University of Pune

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State Level Conference on ‘Microbology in 21st Century’

  

II 

Mission statement

“To Create

Multidisciplinary Best Citizens To Suit

Local, National and International Needs Having

Scientific Temperament, Moral and Ethical Values

and Multifaceted, Proactive Personality

By Providing Excellent Education”

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1

State Level Conference on ‘Microbiology in 21st Century’

25th – 26th February, 2011 Organized by

Department of Microbiology

Modern College of Arts, Science & Commerce, Shivajinagar, Pune, 411005, Maharashtra, India.

Conference Schedule - Friday, 25th

February 2011

Inaugural Function

10.00 am-11.00 am Registration and Refreshment

11.00 am-12.00 pm Inauguration

• DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Pune

• DR. RAMESH S. PARANJAPE, Director, NARI, Pune

• PROF. MRS. JYOTSNA. G. EKBOTE, Chairman, LMC (Junior College), MCASC, Pune

• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Pune

• DR. ARVIND K. PANDE, Prof-In-charge, Department of Biotechnology and Microbiology.

12.00 pm-12.45 pm DR. RAMESH S. PARANJAPE, Key Note Speaker

Director,

National AIDS Research Institute, Pune

12.45 pm-01.30 pm Lunch

Session I

Lectures

01.30 pm-02.00 pm DR. PRASHANT K. DHAKEPHALKAR, Chairperson

Scientist ‘E’, Agharkar Research Institute, Pune

02.00 pm-02.45 pm

DR. S. MOHAN KARUPPAYIL, Invited Speaker

Topic:” Prokaryotes and Eukaryotes: Do they talk to each other?”

Professor and Director, School of Life Sciences, S.R.T.M.University, Nanded

02.45 pm-03.30 pm

DR. PRAKASH R. THORAT, Invited Speaker

Topic:”Decolourization and degradation of textile dyes by microorganisms”

Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.

Oral Presentations

03.30 pm-03.40 pm

OP 01

Candida glabrata infection of the nasal septum – an unusual case report.

Dr. Roma A. Chougale

MBBS, MD, Lecturer in Microbiology, D. Y. Patil College, Kolhapur.

03.40 pm-03.50 pm

OP 02

Prevalence of methicillin resistant Staphylococcus aureus in D.Y.Patil hospital and Research centre.

Dr. Reena A.Dighe

MBBS, DCP, MD, Department of Microbiology,D. Y. Patil Medical College, Kolhapur.

03.50 pm-04.00 pm

OP 03

Bacteriological quality of water, sanitation interventions and health with special reference to diarrhoea.

Mr. Anil M. Garode

Department of Microbiology, Shri. Shivaji Science and Arts College, Chikhli, Buldhana.

04.00 pm-04.10 pm

OP 04

Screening of fungal isolates for lignolytic enzyme, manganese peroxides

Madhuri A. Udata, Joshi A. J., Dr. Mahendra K. Ranjekar.

Department of Microbiology, Dayanand Science College, Latur.

04.10 pm-04.20 pm

OP 05

Biotransformation of Textile Azo Dyes by Aerobic Bacteria.

Ravi V. Kale and Prakash R. Thorat.

P.G.Department of Microbiology and Research Center, Shri Shivaji Mahavidyalaya, Barshi, Solapur.

04.20 pm-04.30 pm

OP 06

A cost-effective protocol for cultivation, microbial and biochemical analysis and high yielding nutritious variety of

mushrooms.

Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P. Anandwani, Jayesh A. Patil, Department of Microbiology,

PSGVPM’s, ASC College, Shahada, 425409, Nandurbar.

04.30 pm-04.40 pm

OP 07

Biosurfactant production by a marine strain of Pseudomonas.

Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Prof. Balu A. Chopade.

Department of Microbiology & Institute of Bioinformatics and Biotechnology, University of Pune, Pune.

04.40 pm-05.00 pm Tea

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2

Conference Schedule – Saturday, 26

th February 2011

Session II

Lectures

10.00 am-10.30 am Refreshment

10.30 am-11.00 am DR. DIGAMBAR V. GOKHALE, Chairperson

Scientist ‘F’, National Chemical Laboratory, Pune

11.00 am-11.45 am

PROF. ARVIND M. DESHMUKH, Invited Speaker

Topic:”Microbial solubilization of metals from waste printed circuit boards”

Head, Department of Microbiology, BAMU, Osmanabad.

11.45 am-12.30 pm

PROF. SUDHIR B. CHINCHOLKAR, Invited Speaker

Topic:”Recent Trends in Microbial Control of Phytopathogens”

Director, BCUD, North Maharashtra University, Jalgaon

Oral Presentations

12.30 pm-12.40 pm

OP 08

Antiplasmid activity of herbal extracts on Methicillin Resistant Staphylococcus aureus

Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar.

Department of Microbiology, K. T. H. M. College, Nashik

12.40 pm-12.50 pm

OP 09

Study of extended spectrum beta lactamase (ESBL) producing Gram negative bacilli in family Enterobacteriaceae.

Dr.Vishwashanti. S. Vatkar, Dr.P.G.Shadija, Dr.S.J.Ghosh

Department of Microbiology, Dr.D.Y.Patil Medical College, Kasaba Bavada, Kolhapur.

12.50 pm-01.00 pm

OP 10

Microbial Bioaugmentation for treatment of industrial effluents in Common Effluent Treatment Plant (CETP)

Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Dr.Seema S.Sarnaik, G.K.Wagh, Dr.Pradnya P.Kanekar

Microbial Sciences Division, MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune.

01.00 pm-01.10 pm

OP 11

Isolation of Rhizobium and Optimization of Growth Conditions for Development of Biofertilizer for Trigonella

foenumgraecum (Fenugreek).

Aasawari S. Pawar and Rajendra Choure.

Department of Microbiology, The Institute of Science, Mumbai.

01.10 pm-01.20 pm

OP 12

Synthesis and characterization of ZnO nanoparticles and their application on textiles as antimicrobial agents and UV

absorbers.

Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija.

Department of Biotechnology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.

1.20 pm-01.30 pm

OP 13

Comparative study on the production of Prodigiosin by Serratia marcescens using various crude fatty acid sources and its

applications.

Pankaj .S. Picha, Sheetal .P. Pardeshi

Department of Microbiology, Modern College of Arts, Science and Commerce, Shivajinagar, Pune.

01.30 pm-02.00 pm Lunch

Session III

02.00 pm-03.00 pm Poster Presentation

Session IV

03.00 pm-04.00 pm

Panel Discussion

• DR. BALU A. CHOPADE, Director, Institute of Bioinformatics and Biotechnology, Pune

• DR. RAJNEESH B. VAIDYA, HOD, Microbiology, The Institute of Science, Mumbai.

• DR. BALU P. KAPADNIS, HOD, Microbiology, University of Pune, Pune.

• DR. RENU BHARADWAJ, Dean, B.J.Medical College, Pune.

• DR. PRAKASH R. THORAT, Executive member, AMI; HOD, Microbiology, Shri Shivaji Mahavidyalaya, Barshi.

• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar, Pune.

• DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology

04.00 pm-04.10 pm Tea

04.10 pm-04.40 pm

Valedictory function

• DR. GAJANAN R. EKBOTE, Chairman, Business Council, P. E. Society, Shivajinagar, Pune.

• DR. RAMCHANDRA V. GADRE, Scientist, National Chemical Laboratory, Pune

• PROF. MRS. JYOSTNA. G. EKBOTE, Chairman, LMC Junior College, MCASC, Shivajinagar, Pune.

• DR. RAJENDRA S. ZUNJARRAO, Principal, MCASC, Shivajinagar , Pune

• DR. ARVIND K. PANDE, Prof. In Charge, Department of Microbiology and Biotechnology, MCASC, Shivajinagar , Pune

• DR. SHILPA S. MUJUMDAR, Head, Department of Microbiology, MCASC, Shivajinagar, Pune.

04.40 pm-05.10 pm Certificate Distribution

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Messag

Dr. ThoraExecutive Association Head, P.G. DeparShri. Shiva Member BFaculty of Solapur UnSolapur, M

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Messag

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State Level Conference on ‘Microbology in 21st Century’

  

V

Message from Head, Microbiology, University of Pune 

Professor B. P. Kapadnis Head, Department of Microbiology, University of Pune, Ganeshkhind, Pune 07

“I am happy to know that the Department of Microbiology, Modern College

of Arts, Science and Commerce, Pune 05, is organizing State Level Conference entitled “Microbiology in 21st Century” on 25th and 26th February 2011.

In today’s Modern world, Microbiology is considered a vibrant and exciting field with many applications and the new and exciting discoveries been made in microbial ecology, molecular genetics, space microbiology etc. With the changing faces of microbes, it is mandatory for us to refine our approaches towards treating the infectious diseases. Genetically tailored medicine is picking up pace and that really gives us a ray of hope in combating the problem of multidrug resistant organisms which is the need of the hour. This conference will present multitude of subjects with a unique blend of modern research in microbiology.

I am certain that this conference will prove to be the perfect platform for the professionals to greatly benefit from the exchange of ideas and also the debates and deliberations related to the explorations of an integrated approach to microbiology.

I wish all the best and success for this conference.” Professor B. P. Kapadnis Department of Microbiology, University of Pune, Ganeshkhind, Pune 07

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Chairma

Dr. G. R. EChairmanBusiness CProgressivShivajinag Chief Org‘State leveMicrobiol25th - 26th

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State Level Conference on ‘Microbology in 21st Century’

  

VII

noted the achievements and reputation of the college. The Govt. of Maharashtra and University of Pune has recently accorded the sanction to increase the intake capacity of the M.Sc. course. The young and highly qualified team of faculty members spares no efforts to keep the students well informed and in updating their knowledge. The students are also reciprocating by their high achievements in academic performance. A large number of students are getting excellent placements in different part of the country & also abroad. Every year several students are also selected in reputed foreign universities for higher studies. Concentrated research and development is required on various micro based technologies that can be utilized to increase crop production and utilize agro waste, manage abiotic stress drug design, for biocontrol of important insect pests and in post harvest technologies

I am certain that the conference will prove to the perfect platform for the professionals to greatly benefit from the exchange of ideas and also, the debates and deliberations related to explorations of integrated approach to Microbiology. I also hope that there will be sufficient knowledge and direction for the young and budding scientists also.

I congratulate the principal of modern college Dr. Rajendra S. Zunjarrao, Dr. Shilpa S. Mujumdar, HOD, Department of Microbiology and organizing team for organizing this conference and wish them a grand success.”

Dr. G. R. Ekbote Chairman, Business Council, Progressive Education Society Shivajinagr, Pune 5.

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Principa

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State Level Conference on ‘Microbology in 21st Century’

  

IX

science. With this notion in mind we had submitted proposal to the University of Pune to organize State level conference on ‘Microbiology in 21st Century’ under its Quality Improvement Programme. We are very glad to communicate you that, in response to our Proposal University of Pune has given approval and consent to organize the International Conference on the said topic.

As it is state level event we have invited and expecting the participation of Resource Persons, Subject Experts, Scientists, Eminent Personalities from the academic field and industry, Faculties and Students from the various districts in Maharashtra. On this occasion I convey my warm greetings and felicitations to all the delegates and wish them a scintillating conference.”

Dr. Rajendra S. Zunjarrao Principal, Progressive Education Society’s Modern College of Arts, Science and Commerce, Shivajinagar, Pune 5.

   

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State Level Conference on ‘Microbology in 21st Century’

  

X

Committees for State Level Conference “Microbiology in 21st Century”  25th and 26th February 2011 

 

Organizing committee Dr. G. R. Ekbote, Chief Organizer and Chairman, P.E. Society. 

Dr. R. S. Zunjarrao, Principal and Convener 

Prof. A. G. Gosavi, Visitor, MCASC, Pune 5 

Dr. A. K. Pande, Prof. In‐Charge, Microbiology  

Dr. (Mrs.) S. S. Mujumdar, Organizing Secretary and HOD, Microbiology  

Prof. A. M. Bhalerao, Professor, Dept. of Zoology 

Prof. S. S. Thengadi, Professor, Dept. of Physics. 

Prof. Y. Thipse, Asst. Prof. Dept. of Electronics 

Ms. S. S. Bhuyan, Asst. Prof, Microbiology 

Mr. V. N. Bobade, Asst. Prof., Microbiology 

Ms. S. P. Pardeshi, Asst. Prof., Microbiology 

Ms. S. P. Bashetti, Asst. Prof, Microbiology 

Mrs. A. A. Devale, Asst. Prof., Microbiology 

Ms. M. J. Amrutkar, Asst. Prof, Microbiology 

Mrs. R. S. Sawant, Asst. Prof, Microbiology 

 

 Advisory Committee   Dr. G. R. Ekbote, Chairman, P.E. Society. 

  Dr. P.S. Chirputkar, Chairman, LMC (Senior College), MCASC. 

  Dr. A. K. Pande, Prof.‐In‐Charge, Dept. of Biotechnology and Microbiology, MCASC, Pune 5. 

  Prof. Mrs. J. G. Ekbote, Chairperson, LMC (Junior College), MCASC. 

  Dr. R. S. Zunjarrao, Principal, MCASC, Pune 5. 

  Prof. A. G. Gosavi, Visitor, MCASC, Pune 5. 

  Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 5.   

  Prof. S. Choudhari, Vice‐Principal, MCASC, Pune 5 

   

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State Level Conference on ‘Microbology in 21st Century’

  

XI

  Scientific Advisory Committee    Prof. Dr. B. A. Chopade, Director, IBB, University of Pune  

  Prof. Dr. B. P. Kapdnis, Head, Dept. of Microbiology, University of Pune. 

  Prof. Dr. R. L. Deopurkar, Professor,   Dept. of Microbiology, University of Pune. 

   Dr. P. K. Dhakephalkar, Scientist ‘E’, ARI, Pune 

   Prof. Dr. Sankunny M. Karuppayil, Director, School of Life Sciences, S. R. T. M. 

University, Nanded 

   Dr. D. V. Gokhale, Scientist ‘F’, Scientist In charge, ICIM, NCL, Pune  

   Prof. A. M. Deshmukh, Head, Department of Microbiology, BAMU, Osmanabad 

  Prof. S. B. Chincholkar,  Director, BCUD, North Maharashtra University, Jalgaon 

  Dr. P. R. Thorat, Head, Dept. of Microbiology, Shri Shivaji Mahavidyalay,  Barshi 

  Dr. H. V. Ghate, Head, Dept. of Zoology, MCASC. 

 

Editorial Committee  Chief Editor:  Dr. R. S. Zunjarrao, Principal  

Advisor:    Dr. A. K. Pande 

     Prof. S. S. Deshmukh, Vice‐Principal, MCASC, Pune 05.  

Members:   Dr. (Mrs.) S. S. Mujumdar 

    Mr. V. N. Bobade 

      Ms. S. S. Bhuyan 

 Travel and Accommodation Committee  Advisor:   Prof. A. G. Gosavi, Visitor   

Members:   Dr. (Mrs.) S. S. Mujumdar 

     Mr. V. N. Bobade 

    Mr. P. S. Warhade 

    Mr. A. A. katkar 

    Mr. V. M. Daundkar 

    Ms M. J. Amrutkar 

    Mr. P. Sabale 

   

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State Level Conference on ‘Microbology in 21st Century’

  

XII

 Technical Assistance Committee  Advisor:   Prof. S S. Deshmukh 

       Prof. A. Sathe,  

Members:   Mr. V. N. Bobade 

    Ms. S. P. Pardeshi 

    Ms. Gitanjali Litake  

       Ms. A. V. Jagdale 

    Mr. A. A. Katkar 

    Mr. V. M. Daundkar 

    Mrs. J. J. Dunde 

     Mr. R. Shawalkar 

    Mr. Manik Jadhav 

 

Registration Committee Advisor:   Mr. R. P. Kale, Registrar   

Members:   Mrs. A. A. Devale  

Ms. S. P. Bashetti 

Mrs. R. S. Sawant 

Mr. R. Dabhade 

Ms. R. Thombare 

Mr. V. Lad 

Mrs. S. Patwardhan 

Mr. V. M. Daundkar 

Ms. A. V. Jagdale 

Mr. Sagar Kudale 

   

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State Level Conference on ‘Microbology in 21st Century’

  

XIII

Finance and Sponsorship Committee Advisor:   Prof. S. S. Thengdi  

Members:   Mrs. A. A. Devale  

    Ms. S. S. Bhuyan 

    Ms. M. J. Amrutkar 

    Mrs. S. Puranik 

    Mr. D. Shah 

       Mr. A. Kakad 

 

Certificate Preparation Committee  Advisor:   Prof. R. N. Ingole   

Members:   Mrs. A. A. Devale  

    Ms. S. P. Bashetti 

    Mr. R. Dhabade 

    Mrs. R. Thombare 

 

Hall Arrangement Committee Advisor:   Prof. S. S. Deshmukh 

Members:  Dr. (Mrs.) S. S. Mujumdar   Mrs. Ranjana Dhotre 

    Ms. S. S. Bhuyan      Mr. Tushar Bharekar 

    Ms. M. J. Amrutkar 

       Ms. S. P. Pardeshi 

       Mrs. A. Sardesai 

       Ms. A. V. Jagdale 

     Mr. A. A. Katkar 

       Mr. V. M. Daundkar 

       Mrs. J. J. Dunde 

       Mr. R. Shawalkar 

      Mr. Prasad Vanage 

      Mr. Rajendra Khapre 

      Mr. Sameer Dhavade 

   

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State Level Conference on ‘Microbology in 21st Century’

  

XIV

Hospitality and Food Arrangement Committee Advisor:        Prof. Y. Thipse 

Members:   Mrs. A. A. Devale  

    Ms. M. J. Amrutkar 

    Mrs. A. Sardesai 

    Mr. A. Kakad 

    Mrs. R. S. Sawant 

    Ms. S. P. Bashetti 

       Mr. P. Sabale 

 

Publicity Committee Advisor:   Dr. A. K. Pande  

Members:   Dr. (Mrs.) S. S. Mujumdar 

     Ms. S. P. Pardeshi 

     Ms. S. P. Bashetti 

 

Session In‐Charge Advisor:   Dr. (Mrs.) Shilpa S. Mujumdar  

Members:   Ms. S. P. Pardeshi 

 

   

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State Level Conference on ‘Microbology in 21st Century’

  

XV

          Volunteers

Aniket Hambir  Khude Rasika R. 

Anuradha Kulkarni  Konde Pallavi 

Baviskar Ravindra Dhondu  Lande lalit Satyawan 

Bedare Monika Kundalik  Londhe Madhuri Hanumant 

Bhong Yogita D.  Malla Shikha 

Bhoodatt Mrinmai R.  Mandal Mamta Rajendra 

Bidkar Monali A  Mane Deepali Sahebrao 

Chaudhari Saurabh Renukadas  Mane Priyanka Vasant  

Chhajed Khushbu V.  Mehendaley Saili Vishwas   

Chothe Nital Syam  Nadivkar Pradnya Nandkumar 

Churi Ruta K.  Naina Gaikwad 

Daithankar Bhagyashree D.  Mazhar Tahaseen 

Dasarwar Shriniwas Vijaykumar  Pallavi Konde 

Deore Poonam Vasant  Patil Datta V. 

Deshmukh Sujit M.  Patil Pratibha Abhimanyu 

Deshpande Manisha M  Pawar Gauri Manohar 

Dhamal Hrishikesh Dilip  Picha Pankaj Sudhirkumar 

Gaikwad Sarika  Punde Priya S. 

Gaikwad Sunny Tanaji   Raut Tushar C. 

Gavhane Supriya S.  Ravindra Baviskar 

Ghole Tushar S.  Rohani Neha Giridhar 

Inamdar Bhagyashree Anant  Sakhare Reshma Ramakant 

Jade Rupali S.  Sanam Vynkatesh Madhukar 

Jadhav Amar Dnyaneshwar  Sen Divya Deepakkumar 

Jadhav Kanchan Janardan  Sheetal Joshi 

Jadhav Shweta M.  Shelar Akshay B. 

Jere Saee S.  Shetty Rashmi Deepak 

Kadam Monika A.  Shrinivas Dasarwar 

Kale Trupti P.  Singh Ajay Pal 

Kalel Preeti V.  Sujata Shinde 

Kanchan Jadhav  Telore Snehal Vilas 

Kasyap Poonam R.  Thorave Varsha J. 

Kamelia Behroozi  Jagtap Bhagyashree 

Tejaswita Pawar  Phulphagar Akshata 

Yadav Priya  Manisha Shelar 

Shelar Prachi  Madhuri Wadkar 

Reshma Yeole   

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State Level Conference on ‘Microbology in 21st Century’

  

XVI

Contents – Oral Presentations Abstracts Title and Authors Page

OP1 Candida glabrata Infection of The Nasal Septum – An Unusual Case Report Roma A. Chougale

57

OP2 Prevalence of Methicillin Resistant Staphylococcus aureus in D. Y. Patil Hospital And Research Centre Reena Dighe and Saral Ghosh

54

OP3 Bacteriological Quality of Water, Sanitation Interventions And Health With Special Reference To Diarrhoea. Anil M. Garode

06

OP4 Screening of fungal isolates for lignolytic enzyme, manganese peroxides Madhuri A. Udata, Joshi A. J., Mahendra K. Ranjekar

74

OP5 Biotransformation of Textile Azo Dyes by Aerobic Bacteria. Ravi V. Kale and Prakash R. Thorat

26

OP6 A cost-effective protocol for cultivation, microbial and biochemical analysis and high yielding nutritious variety of mushrooms. Jogi. M. M., Supriya V. Suryawanshi, Bhagyashree V. More, Nilesh P. Anandwani, Jayesh A. Patil

25

OP7 Biosurfactant production by a marine strain of Pseudomonas. Vijendra A. Kavatalkar, Smita S. Bhuyan, Peehu S. Pardeshi, Balu A. Chopade

81

OP8 Antiplasmid activity of herbal extracts on Methicillin Resistant Staphylococcus aureus Avinash D. Bholay, Anita N. Katkade, Kaveri S. Palekar

11

OP9 Study of extended spectrum beta lactamase (ESBL) producing Gram negative bacilli in family Enterobacteriaceae. Vishwashanti. S. Vatkar, P.G.Shadija, S.J.Ghosh

79

OP10 Microbial Bioaugmentation for treatment of industrial effluents in Common Effluent Treatment Plant (CETP) Snehal B. Bari, Nilesh A. Sonune, Amit R. Sinnarkar, Seema S. Sarnaik, G. K. Wagh, Pradnya P. Kanekar

63

OP11 Isolation of Rhizobium and Optimization of Growth Conditions for Development of Biofertilizer for Trigonella foenumgraecum (Fenugreek) Aasawari S. Pawar and Rajendra Choure

01

OP12 Synthesis and characterization of ZnO nanoparticles and their application on textiles as antimicrobial agents and UV absorbers. Rutuja.R.Phatate, Rebecca.S.Thombre and Sonal.P.Bathija

56

OP13 Comparative study on the production of Prodigiosin by Serratia marcescens using various crude fatty acid sources and its applications Pankaj .S. Picha, Sheetal .P. Pardeshi

45

 

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State Level Conference on ‘Microbology in 21st Century’

  

XVII

Contents – Poster Presentations Abstracts Title and Authors Page PP1 Isolation and Characterization of Listeria species from

environmental and clinical sample Abhay Raorane & Barbuddhe S.B.

02

PP2 Antimicrobial activity of Vinca rosea (Catharanthus roseus L.) and Clematis gouriana against pathogenic bacteria Akshay Borkar, Manoj Bhosale, Shirish Shah, Salokhe S.G., Sonawane H.V

03

PP3 Isolation and Screening of plant growth promoting bacteria from rhizosphere Alka Jagdale,Shradha Bashetti, Smita Bhuyan, Shilpa Mujumdar, Anushka Devale

04

PP4 Isolation of Poly-β-Hydroxyalkanoate Producing Bacteria from waste water and optimization of its production parameters Amar D. Jadhao, Hrishikesh D. Dhamal, Vivek N. Bobade

05

PP5 Characterization of contaminants of cosmetics and their antibacterial sensitivity against synthetic and natural preservatives. Ayushi.A.Chaurasiya And R.B.Vaidya

07

PP6 Study of quorum sensing and quorum quenching in Vibrio fischeri and application in aquaculture Archana J. Marry & Jyoti Mantri

08

PP7 Biodegradation of Triaminotrinitrobenzene (TATB) Abhijit More, Sayali Mahajani, Seema Sarnaik & Pradnya Kanekar

09

PP8 Production of amylase by bacteria isolated from soil sample Bedare Monika & Madhuri Amrutkar

10

PP9 Characterization of thermo-tolerant and acid /alkali tolerant β glucosidase from bacterial isolate Bhalkikar S.B. , Diwan V.A., Ranjekar M.K

12

PP10 Detoxication of Azo Dyes by microbial pure cultures as well as microbial consortia. Bholay A.D., Maid Sneha A., Borse Priti k., Rakte Snehal B

13

PP11 Probiotic properties of Lactobacillus isolates from milk of domestic animals and Commercial available probiotic preparations against enteric bacterial pathogens Bhutada SA & Tambekar DH

14

PP12 Isolation , purification & application of lipase enzyme. Bhadale S. V, Bhalerao M.N, Patil N.N, Ghule P.V & Kulkarni

15

PP13 Antibiotic Production from Bacillus spp. Active against Staphylococcus aureus Chavan K.J, Patil S.D, Ranjekar M.K.

16

PP14 Antimicrobial activity of mangroves against multidrug-resistant (MDR) pathogens. Bholay.A.D, Kurup Sunila .B., Vidhate Pushpa.S., Rahatekar Rohini .R.

17

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XVIII

PP15 Biosorption of hexavalent chromium from aqueous solutions using dead fungal biomass of P. chrysogenum Deep.V.Barot & Varsha.Vaidya

18

PP16 Study of antibiotic resistance patterns in Campylobacter spp. isolated from Pune, Maharashtra De Souza NA, Kamble A, Parkar SD, Kapadnis BP

19

PP17 Antimicrobial Activity of new Sulphanylamide Analogues against Commonly occurring Multidrug Resistant Urinary Tract Pathogens Gauri Devasthale, Alok Vyas, Divya Nair & Jhilmil Ghoshal

20

PP18 Isolation, identification and characterization of bioluminescent bacteria from fish sample and its use as water pollution indicator Gauri Pawar, Deepali Mane, Vyankatesh M. Sanam, Shilpa Mujumdar & Smita Bhuyan

21

PP19 Assessment of genetic diversity among mango varieties by RAPD markers Hrishikesh Joshi, A. R. Desai

22

PP20 Development of microbial biostimulants to enhance fertility of saline soils for sustainable agriculture Indulkar V.S. Siddiqui A.G., Ranjekar M.K

23

PP21 Assessment of Water Purification Potential of Moringa Oleifera Coagulant Protein (MOCP) Jain Reena R & Kadam Shradhha D

24

PP22 Isolation of Bacteriophage against Campylobacter from Poultry in Pune Kamble A, De Souza N, Parkar S, Kapadnis BP

27

PP23 Isolation and Identification of Pathogens of Seafood Penaeus sp. Ketki Nalawade & Rajendra Choure

28

PP24 To study anti-bacterial activity of various medicinal plants against mixed dental flora Khadpekar A & Thombre R.

29

PP25 Screening & Production of Laccase from Fungi Kawade S.S, Diwan V.A., Ranjekar M.K

30

PP26 Studies on bioemulsifier production from Provdentia stuartii isolated from garden soil. Lalit Lande, Smita Bhuyan & Shilpa Mujumdar

31

PP27 Comparative study of bioinoculants prepared from rhizosphere soil and non-rhizosphere soil. M. S. Vrushali ,N.Rutika

32

PP28 Isolation of siderophore producing organism from rhizosphere soil of Adathoda vasica Madhuri Londhe, Smita Bhuyan,Shilpa Mujumdar

33

PP29 Comparative Analysis of Faecal Microbiota from Normal and Obese Wistar rats. Mahesh R Ghule,Rajesh M Chomal ,Deepak R Patil & Yogesh S Shouche

34

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State Level Conference on ‘Microbology in 21st Century’

  

XIX

PP30 Production of bacterial cellulose by Acetobacter xylinum (NCIM 2526) using fruit waste and checking for its application Manasi Karmarkar, Poorva Dabir & Meghana Kukarni

35

PP31 Aureobasidium Pullulans Grown On Various Feedstocks For Comparative Studies Meenakshi Dhar, Nasim Kherad, Vinay Rale

36

PP32 Antimicrobial effect of bombax ceiba Monika More, & Gauri Jadhav

37

PP33 Production and purification of extracellular RNases from Streptomyces venezualae (NCIM 2215). Mokshada Godbole, Neelima Kulkarni & S. S. Deshmukh

38

PP34 Isolation screening for penicillin- v acylase producing organism optimization of culture conditions, effect of media constituents on production of enzyme from B. cereus Neha Naik, Avinash Sunder, Laxmi Nair, Vaidehi Dande & A.V. Pundle

39

PP35 Characterization of plasmid encoded resistance to β-lactam antibiotics of Acinetobacter baumannii AIIMS7, a clinical isolate Neha S. Vora, Shradha B. Nadhe, Riddhi J. Shah & Karishma R. Pardesi

40

PP36 Optimization of growth parameters for ethanol production by yeasts Neelima Kulkarni, Shreyas Kumbhare, Pandurang Lahare, Hussain Khokhawala

41

PP37 Comparative In-vitro Antimicrobial activity of the extract of Gloriosa superba against laboratory and antibiotic resistant clinical isolates of common pathogens Nikita L. Lad and Rajendra G. Choure

42

PP38 Extracellular RNase production and purification from Streptomyces sp. (NCIM 2081) Niruta Y. Killedar, Anupama Sonawane and Sumedh S. Deshmukh

43

PP39 Isolation and characterization of oxalate degrading organism P.S, Patil , Patil Sham A , Bedse,Tanuja D, Jadhav Sandip R .

44

PP40 Oligotyping of antibiotic resistance genes from clinical isolates of Acinetobacter spp. Poojari Pritika, Ratnakaran Neena, Mehta Khyati, Shirode Abhinav, Pardesi Karishma

46

PP41 Optimization of Amylase, production by fungi isolated from rhizosphere soil. Pradnya N. Nadivkar & Sheetal. P. Pardeshi

47

PP42 Reduction of heavy metals from domestic sewage by using Microorganisms Prajakta Bhagwat, Priya Bendre and Vrushali Patil.

48

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State Level Conference on ‘Microbology in 21st Century’

  

XX

PP43 Isolation and characterization of indole acetic acid (iaa) producing organism from rhizosphere of gram (Cicer arientinum). Priyanka Mane, Smita Bhuyan & Dr. Shilpa Mujumdar

49

PP44 Wound Healing Properties of honey. Purnima Ranawat & Jyoti Mantri

50

PP45 Optimization and characterization of lipase production by a selected Bacillus sp. and its application Rahul Brid and Rajendra Choure

51

PP46 Laboratory Study on Bioremediation of Diesel Oil Contaminated Soil from a Garage Rakhi Tari & Shilpa Sabnis

52

PP47 Production of giberrelic acid by soil fungi Ravindra Baviskar, Sunny Gaikwad, Shilpa Mujumdar & Smita Bhuyan

53

PP48 Production of Xanthan gum by Xanthomonas campestris using Industrial and kitchen waste as a production media Repe A.B., Dhutekar S.S,Ranjekar M.K.

55

PP49 Biological synthesis of silver nanoparticles and evaluation of their antibacterial activity Sabah Shaikh & Shilpa Sabnis

58

PP50 Study of ocular microflora, antimicrobial and antiadhesive activity of MPS solutions on ocular isolates. Sagar Vandra & Shilpa Sabnis

59

PP51 Identification and extraction of tobacco mosaic virus (DNA) using molecular techniques Samriddhi R. Sharma & Aparna Das

60

PP52 Phylogenetic analysis of insulin (protein) using bioinformatisc tools Sandeep Gajbhiye, Mr. Sunakar

61

PP53 Microbial degradation of Diaminodinitroethene, a high energy nitroexplosive Sayali Mahajani,Abhijit More, Seema Sarnaik & Pradnya Kanekar

62

PP54 Determination Of Resistance Of Listeria monocytogens Towards Sanitizers And Antibiotics In Presence Of Tween 80 Shahina Shaikh, Abhay Raorane & Deepali Giremar

64

PP55 Antibacterial activity of crude extracts of spices against food borne pathogenic microorganisms. Shikha Malla; Shilpa Mujumdar; Madhuri Amrutkar

65

PP56 Evaluation of antibacterial activity of pigments produced by Pseudomonas aeruginosa Shilpi.R.Chand & R.B.Vaidya

66

PP57 Biodegradation of Hexavalent Chromium Using Microorganisms Singh Viveka , Srivastava Shreya and Gore Suneeti

67

PP58 Study of Synthesis of Silver nanoparticles. Sourabh Mehta, Rebecca Thombre, Shubhangi Puranik.

68

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State Level Conference on ‘Microbology in 21st Century’

  

XXI

PP59 Decolorization of textile dye direct lemon yellow by Burkholderia cepacia Sunil. S. Tiwari & Sangeeta. K. Jangam

69

PP60 Phytochemical and antibacterial screening of solvent extracts of leaves of Bauhinia racemosa Lam. (Caesalpiniaceae) against enteric bacterial pathogens S.B.Dahikar, S. A. Bhutada, D.H.Tambekar, and S. B. Kasture

70

PP61 Viral Diagnosis by using RT – PCR: A case study on Banana Chlorosis Disease S.O. Gupta & B.A. Aglave

71

PP62 Biopulp - an eco-friendly approach S. M. Kulkarni, Navale S. K.,Sulkshane H. C.,Sharma L. P., Khandelwal S.R., Shirke M. D., Kokani N. F., Bhavsar S. P., &. Joshi S. A.

72

PP63 Plasmid Profiling and Antibacterial Activity Of Garlic Extract And Clove Oil On Clinical Isolates Of Pseudomonas aeruginosa Tejlaxmi Inamdar, Milky Sahukar, Kavita Parekh.

73

PP64 Screening of Lipases as potential virulence factor in Acinetobacter species isolated from different hospitals in India. Urvi Doshi & Karishma Pardesi

75

PP65 In Vitro Studies of Antimicrobial Properties of Extracts from Unani Medicinal Plants Vaishali Prabhune, Namra Ghansar, Supriya Bhopale

76

PP66 Lignin degrading peroxidase in biobleaching of paper-pulp mill and textile dye-based effluents V. A. Tile, Borkhataria B. V, Bhalerao V. B, Aher N. S.

77

PP67 Bacterial synthesis of silver nanoparticles and its applications V.A.Tile., S.P.Pawar., W.R.Sayyed., A.V.Bhosale

78

PP68 Accumulation of polyhydroxyalkanoate(PHA) from styrene by Pseudomonas spp and effect of N2 concentraction on PHA accumulation. Vidya N. Udawant, Smita A. Daule & D. D. Giramkar

80

PP69 Utilization of molasses for biosurfactant production by Streptomyces S2 Vynkatesh. M. Sanam, Deepali S. Mane., Gauri M. Pawar, Shilpa. S. Mujumdar & Smita. S. Bhuyan

82

PP70 Isolation, Characterization and Application of Phenol Degraders Uzma T. Momin and Varsha K. Vaidya

83

PP71 Antibacterial Activity of Medicinal Plants against Gastrointestinal Pathogens Pratibha A Patil , Shilpa S Mujumdar, Madhuri Amrutkar.

84

PP72 Utilization of petroleum hydrocarbons by bacteria isolated from soil & water contaminated with engine oil Dalvi, C. S., Satbhai, M. A., Bhutada, S. A.

85

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XXII

PP73 Biodegradation of commercially used insecticides by soil isolates B. A. Karode, N. B. Choudhari, S. S. Patil, P.T. Patil & A. H. Jobanputra

86

PP74 Biodegradation of diesel oil by soil isolates V. R. Kothari & A. H. Jobanputra

87

PP75 Raw wastes as alternative media for Trichoderma: Towards sustainable development Rushikesh Sankpal, Sunil Deshmukh & Vinay Rale

88

PP76 Characterization and biodiversity of Pashan lake water Rahul Nimbalkar & Varsha Kulkarni

89

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XXIII

Extended Abstract 

Prokaryotes and Eukaryotes: Do They Talk to Each Other?

Prof. S. Mohan Karuppayil

School of Life Sciences, SRTM University, Nanded. 431606

A paradigm shift is happening in our understanding of the world of unicellular eukaryotes and prokaryotes. The microorganisms, like bacteria and yeasts are often considered as non cooperative individualistic organisms. In fact, they are very social organisms and in nature they tend to live as communities called as biofilms and often behave like multicellular organisms. Biofilms are multicellular assemblages of cells encased in extracellular matrixes. They are interactive and use small diffusible molecules for communication, through a process referred as quorum sensing. They release small diffusible molecules which parallel with cell density and bind to sensors when they reach a critical concentration. The bound proteins activate the transcription of various genes resulting in behavioral changes. Hundreds of genes are regulated by the quorum sensing or chemical signaling molecules. The genes regulated include those which govern luminance, pathogencity, virulence factors, symbiosis/predation to cite a few. The chemical language used by microorganisms may be species/ genera specific or general. In short, microorganisms can detect the presence and density of not only their own species, but also members of other genera or even kingdom. Most of the time, the biofilms are not homogenous assemblages but polymicrobial. For example, in cystic fibrosis patients Pseudomonas aeruginosa-Candida albicans biofilms are common. Various studies shown that bacteria and fungi are not silent partners in biofilms but are engaged in meaningful dialogues resulting in changes in gene expression and observable behavioral changes. Eukaryotic hosts may recognize the quorum sensing molecules produced by the pathogens and may adapt strategies to handle it. Quorum sensing molecules of the pathogens are shown to elicit responses in the host often in favor of them or vice versa. The prokaryote - eukaryote dialogue is not only of importance to pathogenecity or virulence but may also influence secondary metabolite production, symbiosis and/or predation. Understanding the prokaryote-eukaryote dialogue may open up new opportunities for drug development and also reveal the marvelous biology behind the interactions.

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State Level Conference on ‘Microbology in 21st Century’

  

XXIV

Extended Abstract 

Decolorization and Degradation of Textile Azo Dyes by Microorganisms

Dr. P. R. Thorat

[email protected] Department of Microbiology and Research Center, Shri. Shivaji Mahavidyalaya,

Barshi – 413411, Dist. Solapur, Maharashtra

Water is the most precious divine natural resource that exists on our planet. Environment and life are in very close relationship on earth. Humans must protect these natural resources to lead life happily, but rapid industrial development and population explosion has made the problem of environmental pollution more and more acute. In India maximum population inhabits in villages and slum areas, where there is no safe drinking water supply. Dyes and pigments are used in various industrial processes like, textile, pharmaceutical, cosmetics, food, printing, etc. The textile industry is the largest, indispensable and backbone industries of some under-developed and developing countries in the world. The water required for textile industry is very large. Azo dyes that are used in the textile industries are xenobiotic compounds which contain (-N=N-) linkage and which are mutagenic as well as toxic to living organisms and environment also. Textile industry is confronted with the problem of color removal and effluent salt content reduction. The most pressing environmental problem facing the textile industry is the dye containing waste-water. The dye containing effluent represents highly problematic wastewaters not only because of high COD, BOD suspended solids, toxic compounds, but also because of color (dyes) which make them easily recognized and poses esthetic problems. When dyes are released in the environment, they form toxic compounds which harm the living creatures. Also the dyes color the water reservoirs which reduce the penetration of sunlight in turn leading to eutrophication. Presently there is no economical and efficient means or process to achieve the reduction of these two parameters. Now-a-days dye wastewater is treated by physical and chemical procedures which have many shortcomings. These physico-chemical methods available for the treatment of these synthetic dye-stuff, like flocculation, sorption, electrochemical and oxidative degradation. These physico-chemical methods available for the treatment of these synthetic dye-stuff, like flocculation, sorption, electrochemical and oxidative degradation which have limitations like cost effectiveness sludge or residue formation which is difficult to dispose off and also limited in their applications. Many studies have been focused on the microorganisms that are able to degrade the dyes, suggesting the bioremediation as an environment friendly and cost competitive alternative for dyed wastewater treatment. Decolorization of the synthetic

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State Level Conference on ‘Microbology in 21st Century’

  

XXV

dye using different microorganisms appear to be effective, environment friendly, less expensive and with reproducible biotransformation data. Dye-stuffs are degraded by range of microorganisms in nature but studies are concentrated on bacterial and fungal decolorization of the dye. Release of azo dye in the environment is of great concern due to, colour, toxicity, mutagenicity and recalcitrant nature of the dye, considerable attention has been given in determining the ability of microorganism in decolorization and degradation of the azo dyes. In the present study, acclimatized microorganisms isolated from natural sources were used for the study of decolorization and degradation of the dyes viz. Acid Blue - 113, Acid Red - 1, Reactive Orange - 16, Direct Yellow - 50, Yellow - 4G, Orange - 2R, Methyl Red and Disperse Brown - 3REL. In all total 63 microorganisms were isolated, these isolates showed good decolorization pattern with average of 97.95% in nutrient broth condition. In Half Strength Nutrient Broth condition, the isolates showed 86.33% decolorization of the dyes. The cell free extract of the isolates showed 89.07% decolorization of the dyes. The percent COD reduction of the dyes was determined, the isolates showed upto 88.00% reduction in COD of the dyes. The degradation of the dyes was determined by GC-MS analysis technique. The microorganisms were identified by 16s rRNA analysis technique.

Key Words

Decolorization, Degradation, Aerobic, Bacteria, Azo Dyes, COD reduction

 

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State Level Conference on ‘Microbology in 21st Century’

  

XXVI

Extended Abstract 

Microbial Solubilization of Metals from Waste Printed Circuit Boards

A. M. Deshmukh

[email protected]

Department of Microbiology, B. A. M. U. Subcampus, Osmanabad 413501, Maharashtra

With the rapid development of technology, the problem of older electronic

equipments is increasing. The main reason behind such increase is low rate of recycling which, in turn, is due to the complex nature of such waste. Traditional ways for treating such waste are not economical and they contribute to pollution. The promising answer for the problem is bioleaching.

Studies were carried out to solubilize heavy metals from electronic waste. For this purpose, the consortium was obtained using a simple way, similar to that of ‘top down’ approach. Printed circuit boards (PCBs) were used as representative samples of e-waste. Various concentrations of PCB powder were used (1-5%) for bioleaching and their effects on metal solubilization, changes in pH and concentration of ferrous iron were accessed. Maximum level of metal solubilization was found to be 96.93% Cu, 93.33 % zinc for 10 g/litre of PCB powder and 10.26 % Ni for 30 g/litre of PCB powder. In case of lead, only 0.58% solubilization was achieved when 20 g/litre of PCB powder was used. The precipitate formed during bioleaching was analyzed by SEM EDAX which shows the presence of Tin (59.96%), Cu (23.97%), Pb (9.30%) and Fe(5.92%). The causes of copper precipitation were discussed.

Key Words

Microbial consortium, Printed circuit boards, Bioleaching    

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State Level Conference on ‘Microbology in 21st Century’

  

XXVII

Extended Abstract 

Recent Trends in Microbial Control of Phytopathogens

S. B. Chincholkar, B. L. Chaudhari, P. D. Sarode and M. R. Rane

[email protected] School of Life Sciences, North Maharashtra University, Jalgaon 425001

Progress in molecular biology and analytical methods has helped in understanding of biological control of soil borne phytopathogens. There exists a great diversity among pathogenic fungi and bacteria. Therefore, control of such pathogenic micro-organisms is a great challenge. Plant Growth Promoting Rhizobacteria (PGPR) and Bio-control Agents (BCA) having multiple activities is a proper antidote against such phytopathogens. Earlier practices like – fighting of phytopathogens with soil fumigants and using agricultural practices like – crop rotation, tillage is either harmful or inadequate. The recent progress in technology focuses on knowledge on mechanism of competition of food, space, iron, parasitism, induction of systemic resistance and antibiosis. Recent approaches eg. identification of plant genes which improve plant interaction with disease suppressive bacteria, identification of genetic factors of biocontrol strain which alter/diminish biocontrol feature eg. phase variation, identification of factors produced by phytopathogens which alter biocontrol mechanisms eg. fusaric acid reduce anti-fungal metabolite production and also interfere quorum sensing/cell signaling, manipulation of soil mineral properties to optimize biological control would strengthen the efforts of agriculturists in time and space. Out of available novel biocontrol agents, our laboratory has concentrated on phenazines. Phenazines are heterocyclic, nitrogen containing, brightly colored secondary metabolites produced by a variety of bacteria. They exhibit anti-fungal as well as anti-bacterial activity. We concentrated on Pseudomonas aeruginosa 4365 which secretes phenazine-1-carboxylic acid (PCA) and pyocyanin. Standard protocol for laboratory scale production of PCA and pyocyanin was developed. Both compounds were purified to obtain crystals. This purified material was characterized with the help of UV-Visible Spectroscopy, IR, NMR and HPLC. Anti-fungal activity was studied in vitro. Pyocyanin, though a weak antifungal showed pH dependent inhibition of Scelrotium rolfsii. PCA is comparatively strong antifungal compound and inhibited Fusarium oxysporum, Sclerotium rolfsii and Colletotrichum falcatum at very low concentrations. Scaled up production of PCA at 12 L and 125 L was carried out. An attempt was made to evaluate comparative activity of PCA as a foliar fungicide through field trial on chilli which demonstrated that PCA has, if not better, comparable activity with various commercial chemical fungicides. Process details and other technical information will be discussed in conference.  

 

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Extended Abstract 

 Abstract of the Presentation 

Dr. D.V. Gokhale

NCIM Resource Center, National Chemical Laboratory, Pune 411008, India

Microorganisms, their cells or their replicable parts are the tools for

biotechnology. These microbes are diverse and show numerous metabolic activities leading to products of immense industrial importance. The vast number of microorganisms has been isolated, identified conserved and utilized for benefit of mankind. However, 99% of the environmental microbes cannot be cultured under laboratory conditions. Thus the majority of the microbes thus remain hidden which need to be explored, identified and also conserved. These untapped microbial sources can be exploited using metagenomic approach to obtain novel microbial products. All these microbial strains need to be preserved in pure form without losing their genetic as well as phenotypic identity. Microbial Resource center (MRCs) act as custodians of such microbial diversity and play an important role in the storage and supply of authentic reference cultures for research and development. Microbial culture collections are established in many countries having variety of purposes. There are very small culture collections (200 microbial strains) and also large cultures collection possessing more than 50,000 microbial strains.

NCIM Resource Center is a pioneer microbial culture collection facility in India, which offers services to educational / research institutes and industries. NCIM is a unique resource dedicated to the isolation, collection, preservation and distribution of authentic cultures of industrially important microorganisms. NCIM holds about 3500 microbial strains of bacteria (1500), yeast (550), fungi (1300) and algae (15). The main objective of the facility is to supply authentic cultures to research institutes and industries. The catalogue of microbial strains can be accessed through http://www.ncl-india.org/ncim. Ours is small culture collection compared to ATCC and NCIMB. However, I will discuss some of the achievements emerging out of screening our own microbial sources.

 

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Progressiv

Modeand CDepa

 

Isolation oDevelo

Depa

Trigomedicinal proin its root nodchemical fertorganoleptic qof a Rhizobicultivation cocharacterized 2% NaCl, antemperature ancomposition approach. Tdifferential efseedlings of cdifferent soilstreated with thpots seeded wcontrols, thoug

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

of Rhizobiumopment of Bi

Aasawa

artment of Micro

onella foenumgroperties which ardules. These proptilizers. Biofertiqualities of the lium strain isolaonditions and by analysis of

nd symbiotic pend pH sensitive,for laboratory

These isolates gffect on germinacultivars S1 and, SL1 and SL2. Ghe Rhizobium c

with Rhizobium,gh there was con

Arts, Science 11005

biology

OP1

m and Optimiiofertilizer fo

(Fenugr

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obiology, The Ins

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State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

owth Conditifoenumgraec

houre

e, Mumbai 40003

or its rich dietargen fixation by

balanced nitrogenntain the nutri

udy describes theenugreek, optimiofertilizer. Isol0oC tolerance, tosolates were fo8.0, respectivelyd using Plackeum were testedth promotion of ates as well as oth the seedlingsere untreated. In

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Conference on

1st Century February 2011 tract Book

Page 1 

ions for cum

32

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n supply as itional and e screening

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in vivo in s S1 and S2,

n pot assay, ninoculated ants.

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Progressiv

Modeand CDepa

 

Isola

1Depar

Listehumans and apresence of Lclinical sampgenetical studmonocytogeneFurther seroty4b serotype aother ListeriaIsolates wereCiprofloxacinChlorampheni

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ation and ChEnviron

Abhay

rtment of Microb

eria spp. are ubianimals. 14 envListeria spp. Oules, 25 (18.79 dies confirmed es. CAMP and yping study of thand 6 were 1/2b

spp. No enviroe sensitive tow, Vancomycin icol, Penicillin, G

Listeria spp, A

Arts, Science 11005

biology

P

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abhay.micro@biology New Arts

Ahmednag2ICAR, Ela, Old

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PP01

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arbuddhe S.B

@gmail.com s Commerce andgar,

d Goa, Goa

ure. Out of 8 sp133 clinical sam

nmetal samples, ive for Listeria) were pathoge

were performed nic strain revealer species detectes contained path

biotics viz. Amintermediate r

ords MP test, Serotyp

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Species frommple

B.²

d Science Colleg

pp. 2 are pathomples were scre2 (14.28%) andspp. Biochem

enic strains of to confirm the ed that 5 isolatesed were L. ivanhogenic spp. of mpicillin, Doxyresistances tow

ping, AST.

Conference on

1st Century February 2011 tract Book

Page 2 

m

ge,

ogenic to eened for d of 133

mical and Listeria isolates.

s were of novii and Listeria.

ycycline, ward the

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Progressiv

Modeand CDepa

 

Antimicro

Aksha

1Deparm2Dept. o

historicaL. has endemicdermatopcardiac methanowater exconcentr50mg/mwere cheConcentcompoun

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

bial Activity Clematis Gou

ay Borkar, Ma

ment of Zoology, f Microbiology,

Vinca roseaally been used towound healing

c medicinal planpathy, blood disdisorders. Ethan

ol and water extrxtract of Catharation was studie

ml, 100mg/ml, 1ecked for Minimtration. Then thnds by Thin laye

Arts, Science 11005

biology

PP0of Vinca Ros

uriana agains

anoj Bhosale,Sonawan

AkshayBorkar1Annasaheb MagAnnasaheb Mag

a (Catharanthuso treat a wide as

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02 sea (Catharanst Pathogenic

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@gmail.com gar College, Hadgar College, Had

s roseus L.) ansortment of dise

abetic patients. ghats and India,

wound healing,water extract ogouriana was st

s L. of 50mg/ml and water extraentration were sConcentration are evaluated tophy.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

nthus Roseusc Bacteria

h, Salokhe S.G

dapsar, Pune- 41dapsar, Pune-411

nd Clematis goueases. Catharant

Clematis gouriused in the tre

viral fever, heaf Catharanthus tudied. Ethanol eml, 100mg/ml, act of Clematis gstudied. All plaand Minimum Bo find out phy

Conference on

1st Century February 2011 tract Book

Page 3 

s L.) and

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1028. 1028.

uriana has thus roseus iana is an eatment of adache and roseus L.,

extract and 150mg/ml

gouriana of ant extracts Bactericidal ytochemical

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Progressiv

Modeand CDepa

 

Isolation

Alka Jag

Department o

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Phospha

 

 

 

 

 

 

 

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

and Screenin

gdale, Sharad

of Microbiology,

t growth promotplant growth. Gd there increas

for the devellubilization activfarm soil locatedhe further studiof Staphylococctivity. Staphylocoroduction.

ate solubilization

Arts, Science 11005

biology

PP0ng of Plant G

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ha Basetti, SmAnushka

, Modern college

ting bacteria (PGGiven the negasing cost, the lopment of susvity and 6 straind near Saswad. Tes and identifie

cus hominis cuoccus hominis a

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03 Growth Promophere

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e of Art, Science

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stainable agricuns showing nitroThe isolate showed as Staphylocoulture with lignlso showed posi

ords

ion, plant growth

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

oting Bacteri

, Shilpa Muju

e and Commerce,

nd rhizosphere baental impact of

as natural feulture. 20 strainogen fixation actwing both the actoccus hominis bnite showed plaitive Siderophore

h promoting bac

Conference on

1st Century February 2011 tract Book

Page 4 

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, Pune 05.

acteria that f chemical rtilizers is n showing tivity were tivities was by API kit. ant growth e and Indol

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Progressiv

Modeand CDepa

 

Isolationwaste

Ama

Departme

Polyhsynthetic plastdegraded comactivities of numerous baclimited concestudy is to isoβ- hydroxyalkthe growth parwater containicollected. It iDetection of PThe media opone of the vaeither one of tammonium Ph7.0 and 8.0), t24hr, 48hr anddesignated as Among these growth parampromising PHindependent e48hr, temperaof PHA by Wabove concent

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

n of Poly-β-He water and o

ar D. Jadhao,

ent of Microbiol

hydroxyalkanoattics because of t

mpletely to CO2

microorganismscteria as an int

entrations of nutlate the bacteria

kanoates and therameters such asing mostly sewais screened for bPHA production ptimization was arious carbon sothe Nitrogen souhosphate and yetemperature (ambd 72hr) were opt

Wi1 to Wi30 (four cultures W

meters for maximHA accumulatinexperiments 1g %ature 32oC and pWi2, Wi4 and Wtrations except p

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Arts, Science 11005

biology

PP0Hydroxyalkan

ptimization o

Hrishikesh D

viveknbobade@logy, Modern Co

Shivajinagar

tes are considertheir similar phyand water und

s. Poly-β-hydroxtracellular carbotrients with excfrom waste wat

eir characterizatis pH, temperaturage sample from bacteria produciwas done by Sucarried out usin

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Wi2, Wi4, Wi8, mum PHA prodng bacteria. A% glucose, 0.1

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xyalkanoate, PHA

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Published on 25t

04 noate Producof its product

D. Dhamal, Viv

@gmail.com ollege of Arts, Scr, Pune 5.

ed good substituysical and chem

der natural envirxyalkanoates (Pon and energy cess carbon. Theter and that are con. The study are, time, nutrientSiddheshwar G

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o shows maximum production of P

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State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

cing Bacteriation paramet

vek N. Bobad

cience and Comm

utes for petroleuical properties aronment by the PHA) are synth

storage compoe objective of t

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Ghat, Mulla riverhydroxyalkanoa

ng and Spectropmedium contain

ose, Maltose, Sude, Ammonium

meters such as pHand 32oC) and tThe bacterial isore found to prodselected for ev

hese are found tameters evaluat

m phosphate, time for maximum um production PHA is 8.0

of growth param

Conference on

1st Century February 2011 tract Book

Page 5 

from ters

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merce,

um derived and can get

enzymatic hesized by ound under the present

ucing poly -to optimize etc. Waste

r, Pune was ate (PHA).

photometry. ning either

ucrose, and sulfate and

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me of about production of PHA at

meters

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Progressiv

Modeand CDepa

 

Bacterio

Department

The pprimarily chiland poor sanibenefit child quantity of wdisposal of thcontaminated bacteria at doof foods, at wdisposal of hudiarrhoea whithese agents, necessary to btransmission bwhereby childrinking wateimportant rolesupplies espec

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ological QuaHealth with

of Microbiology

primary justificadren, who suffeitation. In develhealth: an impr

water provided he human excret

with the infectises below those

which time the buman excreta shoich are eliminate

do not the tobreak the faecal by water and sadren’s health c

er support the stue in determinincially where the p

Arts, Science 11005

biology

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ation for the decer because of inaloping countriesovement in the and used and pta. Diarrhoea caious agent of th

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r, Sanitation Iference to Di

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on of these pathvement is probaed. The bacterisposal appears toealth in developiarrhoea is high.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Interventionsarrhoea.

College, Chikhl

prove the health ontaminated watypes of the servg water, an incrnitation facilitieing the ingestioater containing p

may be used for ptiply in the foodf major infectioun, the primary edisposal of thei

hogens. Interruptably the major miological examio consistently pping areas, than

Conference on

1st Century February 2011 tract Book

Page 6 

s and

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Progressiv

Modeand CDepa

 

CharaAntibacter

Dep Micr

production anthe cosmetic permanent, vadomestic envicause spoilagethe consumer the type of coload was founproducts and (2009). The coon the basis ofof isolates (20propyl parabeduring succesmineral salts mvarious concegrowth of foupresence of prestablished thThus, it was cosmetic produse of essentifor the growibecause of incthe antibacterithe contaminavariation in dHowever, limisolates. Concspectrum of ac

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

acterization orial Sensitivit

Ayushi.

apartment Of Mic

obial contaminand filling, and du

unit is openedariable and additironment and the of the productworldwide. The

ontaminants prend to be higher102cfu/g or ml fontaminants werf morphological

0) were studied inen as sole sourcsive enrichmentmedium by obseentrations (0.2-2ur isolates and ireservative as co

hat the propertynoted that the

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me, eucalyptus anclusively, it wasction.

Arts, Science 11005

biology

Pof Contamin

ty against Syn

A. Chaurasiy

ayushi.chaurasiyacrobiology, Instit

ants in cosmetiuring the use by d until the contive microbial cohe consumer's bts and when patherefore, the studysent in various

r than the permifor eye based prre isolated, mainand biochemica

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Published on 25t

PP05 ants of Cosmnthetic and N

ya and R. B. Va

[email protected] tute Of Science, M

ic products havthe customer. F

nsumer finishesontamination of tbody. Contaminahogenic represeny was conductedintact and in usissible limit of roducts as per B

ntained on tryptonal characteristicse preservatives s

added separatelyadation of preserhydrolysis for eiparaben was st

d that the growts absence. The pof the paraben

were not successm of antibacteris in cosmetics isngredients fromlly harmful syntoils was screenend to be antibaof them showedexhibited inhibiat the natural es

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

metics and ThNatural Prese

aidya

Mumbai 400032

ve two origins From the momens the product, the cosmetic cauating microorgannt a serious hea

d to determine thse products. The103cfu/g or ml

Bureau of Indianne soya agar and. The biodegrada

such as methyl py to mineral salrvatives was conight isolates. Thtudied colorimeth was slightly aplasmid curing eis chromosoma

sful in preservatial activity. Nows gaining momen

m natural sourcethetic additives.

ed against the 20acterial in actiod no antibacteriaitory action agaissential oils wer

Conference on

1st Century February 2011 tract Book

Page 7 

heir ervatives

2

i.e. during nt in which there is a

used by the nisms may

alth risk for he level and e microbial for topical

n Standards d identified able ability

paraben and ts medium nfirmed on he effect of etrically on affected in experiment

al encoded. tion of the w days, the ntum, both s and also Therefore, isolates of n showing al activity. inst all the re better in

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Modeand CDepa

 

Study of Q

Totaltons and aquaquaculture inprawn cultureculture subsecwhich are bioconventional limited succesdrugs has ledantimicrobial bacteria (probinhibit their prconfirmed as their morpholculture showe.When luminebroth +Vibrioindicating quoshrimp’s intesfree filtrate ofbroth luminescould inhibit assay, by welisolates again14mm, 16mmgrowing VibriVibrio decreasfound to be nenvironment. probiont to ov

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Quorum Sensand A

Archa

l fish productionuaculture accounndustry is beset e industry. Disector. Major aquoluminescent baapproaches likess in the prevend to emergenceresistance to hu

biotics), which roliferation. BioVibrio by platin

logy. They wereed maximum bioescence induced o culture it worum sensing dstine & identifiedf a cocktail of thscence was delathe Vibrio isola

ll-diffusion methnst Vibrio cultur

m. To check the aio and the Bacilsed after 6 hrs &non-hemolytic wThe Bacillus iso

vercome Vibrio in

Arts, Science 11005

biology

P

ing and QuoApplication i

ana J. Marry a

Sophia Colleg

n in the world mnts for 47%. Hby bacterial and

ease has becomeuaculture pathogacteria commone use of disinfetion or cure of ae of more viruuman pathogensare environmen

oluminescent bacng on Thiosulphe identified as Voluminescence in

cell free supernwas observed thdue to autoindud tentatively as b

hese 5 isolates wayed indicating ate when testedhod, using indivre showed zoneantagonistic potellus culture toge

& there was no grwhen grown on b

olates can thus nfection in shrim

Mi

Published on 25t

PP06

rum Quenchin Aquacultu

and Jyoti Man

ge, Mumbai

market is estimateHowever, in mod viral diseases, e a limiting fac

gens are Vibrio nly found in aquectants and antimaquatic diseases

ulent pathogens s. In this study, ntally safe to dicteria were isolahate citrate bile Vibrio fischeri bn 10 hours whennatant broth was hat luminescenc

uction,. Five isobelonging to Ba

was added to the quorum quench

d by cross-streakvidual cell free ees of inhibition ential, co-cultureether & it was forowth at 48 hrs. blood agar thus be used individ

mp aquaculture.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

hing in Vibrioure

ntri

ed to be above 1st of the Asianespecially the s

ctor in prawn aharveyi & Vibruatic environmemicrobial drugs

s. The use of anand eventual twe tried using,

isplace the pathated from squid .

sucrose agar anby biochemical n grown in sea wadded to a fresh

ce occurred witolates were obtaacillus species. WVibrio culture inhing. All these king method. Aextract of the fivof 12mm, 15m method was per

found that viableThe Bacillus isocan be safely u

dually or as a co

Conference on

1st Century February 2011 tract Book

Page 8 

fischeri

110 million n countries shrimp and and shrimp rio fischeri ent. So far s have had ntimicrobial transfer of , beneficial hogens and .They were

nd studying tests .This

water broth h sea water thin 6 hrs ained from When a cell n sea water

5 isolates Antagonism ve Bacillus

mm, 13mm, rformed by e counts of olates were used in the ocktail as a

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Modeand CDepa

 

Bi

Abhijit Mo

Micro Envirthe explosivesThe nitroexplshowing symbiodegradation1,3,5-triaminobenzene ring attached, alterby HEMRL, Pstarting matetrichlorotrinitramination stephigh nitrate, aTATB is knownitro group dioxygenase ethe present stufrom TATB pOut of these, TATB as solmedium at therange of 20-3Mingioli’s syn

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

odegradation

ore, Sayali Ma

bial Sciences Di

ronmental contas industry. Howlosive as well amptoms as kidn of recently d

o-2,4,6-trinitrobestructure with t

rnately to the rinPune is a two-serial. In firstriobenzene (TCp. Wastewaters gammoniacal nitrown as a recalciton the aromati

enzymes involveudy, total 18 baproduction site u

only two bactele source of nite concentration o30% and nitratenthetic medium s

Arts, Science 11005

biology

PP0n of Triamino

ahajani, Seem

ivision, Agharka

amination of nitrever, global proas their environdney trouble, developed insenenzene (TATB)three nitro groung. The current tage process usit nitration steTNB) which thgenerated duringogen and chloridtrant compound ic ring, an arr

ed in the microbacterial isolates wusing enrichmenerial isolates antrogen when incof 100 mg/l. Thee in the range supplemented w

Mi

Published on 25t

07 otrinitrobenz

ma Sarnaik an

ar Research Insti

rocompounds is oduction and expnmentally transfjaundice etc.

nsitive high ene). It is an aroups (NO2) and tprocess for the ing 1, 3, 5-trichep, trichlorobehen is further g their productiode content causibecause of the

rangement that ial metabolism owere obtained frnt, adaptation annd one isolate ocorporated in De isolates showeof 20-35% wh

with 0.05% pepto

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

zene (TATB)

nd Pradnya K

itute, Pune 411 0

associated princplosive use is unformed products

Present studieergy nitroexplosomatic explosivthree amino (NHproduction of T

hlorobenzene (TCnzene is conconverted into

on are highly aciing environmentsymmetric localimits attack

of aromatic comrom soil samplend soil baiting tof actinomycete Davis Mingioli’sed removal of TAhen incorporatedne.

Conference on

1st Century February 2011 tract Book

Page 9 

Kanekar

004

cipally with navoidable. s are toxic es include sive (IHE) ve, having H2) groups

TATB used CB) as the

nverted to TATB in

idic having tal hazards. ation of the

of classic mpounds. In es collected techniques.

could use s synthetic ATB in the d in Davis

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Progressiv

Modeand CDepa

 

Produ

Departme

Starcfermentation, various samplpresent investAgricultural Cmost promisinfurther study. The optimum observed at 1a8 for optimum

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ction of Am

Bedare

ent of Microbiol

ch degrading batextile and pap

les has manifoldtigation bacteria

College, Pune. Sng amylase produ

Growth patterntemperature for

and 2% starch com growth. Maxim

A

Arts, Science 11005

biology

PP0

mylase by BSamp

Monika and M

logy, Modern CoShivajinagar, P

acteria are mosper. Thus isolatd importance foal strains were i-5 & Si-6 bacteucing bacteria ams as well as optir these strains woncentration respmum enzyme acti

Key Wo

Amylase, soil, sta

Mi

Published on 25t

08

Bacteria Isople

Madhuri Amru

ollege of Arts, ScPune 411 005

t important forting and manipur various biotecisolated from soerial isolate frommong 15 isolatesimum growth cowas 37oC, wherepectively. The pHivity was observ

ords arch degrading

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

olated from

utkar

cience and Comm

r industries suchulating pure cu

chnology industroil sample colle

m soil were founs & these were sonditions were deas maximum gH range was fouved at this condit

Conference on

1st Century February 2011 tract Book

Page 10 

Soil

merce,

h as food, ulture from ries. In the ected from

nd to be the selected for determined. growth was und to be 7-tion.

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Progressiv

Modeand CDepa

 

Antiplas

The mproblem and mgenetic determbacteria. The and aqueous clinical isolatewere isolatedAzadirachtaincuring activitygrowth in the derivatives waaqueous and MRSA. Such making them may be a sodevelopment a

Antibacteria

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

mid Activity

A.D Bho

Department of

methicillin resistmajor challenge

minants that conpresent investigextracts of leaves. Active fracti

d from the orgndica, Annonasqy of organic ext presence of antas further confirmorganic extractplasmid loss r

sensitive to low ource to develoand spread of pla

al Activity, Antib

Arts, Science 11005

biology

OP0

of Herbal ExStaphylococc

lay *, Katkad

adbholay@gf Microbiology, K

tance Staphylocoe to the global dnfer resistance togation was underves of different ions demonstratganic and aquequamosa, Ocimtracts wasdetermtibiotics. The phmed by agarose s of leaves of eversed the mu concentrations

op antiplasmid asmid borne mul

Key Wobiotic Resistance

Plasmid C

Mi

Published on 25t

08

xtracts on Mcus aureus

de A.N,Paleka

gmail.com K. T. H. M. Colle

occus aureus (Mdrug discovery po antibiotics arertaken to investiplants to cure

ing antibacterialeous extracts omumsanctum, Pmined by evaluathysical loss of pgel electrophoretested plants cu

ultiple antibioticof conventionalagent of natu

ltiple antibiotic r

ords: e, Antiplasmid ACuring

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Methicillin Res

ar K.S.

ege, Nashik

MRSA) is a seriprogrammers. M

e located on R-pigate the ability R- plasmids fro

l andant plasmidof shade dried Pongamiapinnatating the ability oplasmid DNA inesis.The active fured the R-plas

resistance in cl antibiotics. Theral origin to cresistance.

Activity, Herbal

Conference on

1st Century February 2011 tract Book

Page 11 

sistant

ious health Most of the plasmids in

of organic om certain d activities

leaves of a. Plasmid of bacterial n the cured fractions of mids from uredstrains

ese extracts control the

Extracts,

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Progressiv

Modeand CDepa

 

Charact

1D 2Hea

Cellu

compounds in

promote solu

application of

vegetables to

removing bitte

study is to iso

water. These

parameters of

Cel

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

erization of Tβ-Gluc

Bhalkikar S

Department of Mad, Department o

ulose is most abu

n terrestrial bio

ubilization of c

f β glucosidase i

aglycone moie

er compounds fr

olate bacteria fro

isolate would

f these crude filtr

llulose, β-Gluco

Arts, Science 11005

biology

PP0

Thermo-Toleosidase from

S. B., Diwan V

Microbiology, Daof Microbiology

undant on earth c

omass. Endo and

crystalline cellu

include converti

eties, detoxificat

rom citrus fruit j

om raw material

be screened for

rate would be det

Key Wosidase , Acid /A

Mi

Published on 25t

09

erant and Acim Bacterial Iso

V. A.1, Ranjeka

ayanand Science , Dayanand Scie

constituting abou

d exo glucanas

ulose into solu

ing phytoestroge

tion of cassava,

uices or unripe

l that is rice stra

r β glucosidase

tected.

ords lkali Tolerant, T

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

id /Alkali Toolate

ar M. K..2

College, Latur ence College, La

ut half of the car

es act synergist

uble sugars. B

en glucosides in

, aroma enhanc

olives. The purp

aw and unai ther

e production and

Thermo-Tolerant

Conference on

1st Century February 2011 tract Book

Page 12 

lerant

atur

rbonaceous

tically and

iotechnical

n fruits and

ement and

pose of this

rmal spring

d different

t

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Progressiv

Modeand CDepa

 

Detoxica

Bhola

Azo as leather, texgroup of dyeshas been estimto the fibers astructural propmicroorganismfound to catamicrobial speselected for fufour species c(Reactive red-(Malachite grphysiological yellow- 145 woptimization decolorizationcultures showpure cultures tand detoxicatiseen within lesproduces varistudies. Detoxconsortia provfurther charac

Detoxic

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ation of Azo D

ay A. D., Maid

Department of

dyes are used wtile, plastics, pap

s used in industrmated that 10% and is therefore perties that are

ms such as aeroalyze dye decoloecies were isolaturther study out can decolorize -195, Reactive breen) and ‘simuand metabolic a

was selected as mof physicochem

n within three dws 80% of decolo

the bacterial andion. Promising rss period of timeious ‘aromatic xication studies ved to be significterized by activa

cation, Azo Dyes

Arts, Science 11005

biology

PP1

Dyes by MicrMicrobial C

d Sneha A., Bo

of Microbiology,

widely in the manper, hair care prory representing mof the dye stuff released in to thnot easily degra

obic and anaeroborization and dyted. After screeof them two ar

and detoxify ‘Ablack-5, Reactiv

ulated effluent’ (aspects of decolomodel azo dye umical paramete

days under statioorization withind actinomycetal results were obtae than that of puramines’ which were carried oucantly efficient tated sludge proce

Key wos, Reactive Yello

Mi

Published on 25t

10

robial Pure CConsortia

orse Priti k., R

K.T.H.M Colleg

nufacture of varoducts and cosmmore than half of during this dyinhe sewage or enadable under nabic bacteria andye detoxication.ening the most re bacteria and tAcid dyes’ (Metve yellow-145), (Mixture of 10

orization and detunder static and

ers bacterial puonary conditions

n 5 days under sconsortia are alsained about 95%re cultures. The b

were confirmeut in vivo by seethan the individuess for heterogen

ords ow, Consortia, F

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Cultures as w

Rakte Snehal

ge, Nashik.

rious consumer gmetics. Azo dyes of the annual prong processes dos

nvironment. Azoatural conditionsd actinomycetes

From textile efefficient four ctwo are actinomthyl red), ‘Reac‘Tryphenyl medyes). In orde

toxication procesd shaking conditure cultures shs and Actinomyshaking conditioso tested for dec

% to 96% of decbiodegradation oed by ‘TLC’ aed germination. ual isolates, whicneous dye efflue

FTIR, Decoloriza

Conference on

1st Century February 2011 tract Book

Page 13 

well as

B.

goods such are largest

oduction. It se not bind dyes have

s. Different have been

ffluents 25 ultures are

mycetes. All ctive dyes’ ethane dye’ er to study ss Reactive tions. After hows 90% ycetes pure ns. Instead

colorization colorization of azo dyes

and ‘FTIR’ The mixed ch could be ents.

ation

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Modeand CDepa

 

Probiotic PAnimals a

1Department o2Departm

Probi

resistance aga

120 milk sam

isolates were

recognized as

excellent pro

potential with

plus, P-Biotic

plantarum (M

exhibited exc

and standard p

from milk of

infections and

Probiotics, L

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Properties ofand Commer

En

Bhutad

of Microbiologyment of Microbio

iotic is the group

ainst intestinal p

mples (40 each

identified as Lac

s prominent pr

obiotics. These

h commercial pro

s kid, Gastroline

MTCC 2621) an

ellent probiotic

probiotic bacteri

f domestic anim

d contributes hea

Lactobacillus, A

Arts, Science 11005

biology

PP1

f Lactobacillucial Availabl

nteric Bacteri

da S. A.1 and

sabhutada13@, New Arts, Comlogy, S.G.B. Am

p of microbes th

pathogens and in

from buffalo, c

ctic Acid Bacter

robiotics, among

excellent prob

obiotic preparati

e, Pre-Pro kid an

nd L. rhamnos

characteristics

ial strains. Study

mals can be he

alth benefits to co

Key Wo

Antibacterial Acti

Preparat

Mi

Published on 25t

11

us Isolates frole Probiotic Pial Pathogens

Tambekar D.

@gmail.com mmerce and Sciemravati Universit

hat may be helpf

n the prevention

cow and goat)

ria (LAB). Out o

g them 3 isola

biotics were co

ons such as Spo

nd standard prob

us (MTCC 104

than commercia

y suggested that

lp to prevent o

onsumers.

ords

ivity, Bacterioci

tions

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

om Milk of DPreparations s

. H.2

ence College, Ahty, Amravati, 44

ful directly for e

n of diseases. A

were analyzed

of these 11 isola

ates were ident

ompared their p

orlac powder, La

biotic bacterial s

48). The isolat

al probiotic prep

use of probiotic

or control the i

in, Commercial P

Conference on

1st Century February 2011 tract Book

Page 14 

Domestic against

hmednagar, 44602,

enhanced

A total of

and 110

ates were

tified as

probiotic

actoBacil

strains L.

ed LAB

parations

c bacteria

intestinal

Probiotic

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Progressiv

Modeand CDepa

 

Isola

Bhadale

Departmen

Lipas

most widely u

current and p

industrial enz

pharmaceutica

(triacylglycero

or together wi

extracellular,

oil interface.E

In general, the

the use of wa

lipase produce

is an inexpe

tributyrin wer

and Rhodam

presence of u

wavelength o

precipitation m

enzyme was s

fungi and acti

various substr

producer by a

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ation, Purifica

S. V., Bhalera

b

nt of Microbiolog

ses are used in t

used as biocataly

potential applic

zyme. These ar

al preparations,

ol acylhydrolase

ith esterases (car

hydrolytic enzym

Even though Lipa

e use of water so

ater in-solublesub

ers is done by us

ensive substitute

re added to min

mine B agar te

uranyl ions, yie

of 350nm. Enz

method. Molecu

studied. Most co

inomycetes. The

rate selectivity i

actinomycetes f

Arts, Science 11005

biology

PP1

ation and Ap

ao M. N., Pati

bhadalesujeet123

gy, Annasaheb M

the food, dairy, d

yst in the field

ation lipases ar

re currently us

flavour compo

e; EC 3.1.1.3) ar

rboxylic-ester hy

me because they

ases and esterase

oluble substrates

bstrates is consi

sing tributyrin(9)

e for trioleoylg

nimal medium an

est was done fo

elding orange fl

zyme purificatio

ular weight was d

ommercially ava

e search for new

s still going on.

from hot water.

Mi

Published on 25t

12

pplication of L

il N. N., Ghule

[email protected]

Magar College, H

detergent, cosme

of organic chem

re considered t

ed in the prod

ounds and other

re produced by a

ydrolases; EC 3

y act on substrat

es differ in their

is considered di

idered diagnostic

) or Tween 80(1

glycerol. Then

nd activity were

or confirmation.

fluorencent comp

on was done

determined by S

ailable lipases ar

w lipases with g

Hence this wor

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Lipase Enzym

e P. V. and Ku

Hadapsar, Pune

etic, tanning ind

mistry. In the vie

to be promising

duction of fine

r food additativ

actinomycetes e

.1.1.1).Lipases a

tes providing an

r ability to act at

iagnostic for est

c for Lipases Sc

2) as a substrate

various concen

e studied . Tribu

Rhodamine B

mplexes with an

by Ammonium

SDS-PAGE. App

re synthesized b

greater thermost

rk is being done

Conference on

1st Century February 2011 tract Book

Page 15 

me

ulkarni

411028

dustries and

ew of their

g class of

chemical,

ves. Lipase

either alone

are unusual

n water and

Interfaces.

erases, and

creening of

e .Olive oil

ntration of

utyrin agar

is used in

excitation

m sulphate

plication of

by bacteria,

tability and

e for lipase

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Progressiv

Modeand CDepa

 

Ant

Post Gradua

The

meaning 'life'.

spp. Produce m

-methyllanth

antibiotics, an

peptide antibi

against Gram

methicillin-res

Staphylococcu

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

tibiotic Produ

Chavan

ate Department o

word antibiotic

. There are man

many type of an

hionine. It is cla

nd is produced b

otic isolate from

m-positive bac

sistant strains.Th

us spp.

Arts, Science 11005

biology

PP1

uction from B

Staphylococc

K. J., Patil S.

of Microbiology,

comes from th

ny Bacteria can

tibiotics. Mersac

assified as a mem

by a species of B

m fermentations

teria, particula

his Meracidin an

Key woMersacidin, Ba

Mi

Published on 25t

13

Bacillus spp. A

cus aureus

D., Ranjekar

, Dayanand Scie

he Greek anti m

produce antibio

cidin is a new pe

mber of the pro

Bacillus. It is pe

of a Bacillus sp

arly Staphyloco

ntibiotic is activ

ords acillus spp.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Active agains

r M. K.

ence College Lat

meaning 'against

otic. Among thes

eptide antibiotic

oposed lantibioti

eptide antibiotic

pecies. It is ma

occus aureus,

ve against many

Conference on

1st Century February 2011 tract Book

Page 16 

st

tur, India.

t' and bios

se Bacillus

containing

ic group of

. This new

ainly active

including

y strains of

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Progressiv

Modeand CDepa

 

Antimicr

Bholay A.

Depart

The marine tidal grasses whichthe most proddirectly or indMangroves arerosion and cylike tannin exuses and severunexplored. Tof India are Xylocarpus ggymnorrhiza, and mangrovantifungal comflavonoids, althe presence fungal strainsPseudomonas were resistanAmphicillin +different conc,become sensiare more potenpathogens is cof mangrove wresistance proloss of plasmsynergestic eantibiotics are

Mangroves, M

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

robial Activity

D., Kurup Sun

tment of Microb

term mangroveforests compris

h are associated iductive and diverdirectly dependere highly productyclonic conditioxtraction, paper ral other by-pro

The most domina:- Rhizophora

granatum, X.moB.parviflora, S

ve associates mpounds. They akaloids and tannof new antibac

s. The MDR p , Staphyllococcu

nt to drugs lik+Sulbactam,Spacentration in twoitive to the abovnt as compared t

carried out in Muwas obtained by

operties are assomid were confireffect of mixede under experime

Multidrug-Resist

Arts, Science 11005

biology

PP1

y of Mangrov(MDR) Pa

nila B., Vidha

adbholay@g

iology ,K.T.H.M

e is also used sing of trees, sin strands or grorse in the world ent on mangrovetive and econom

ons. This uniqueand pulp, firew

ducts.The antimant mangrove spmucronata, R.alluscensis, Avic

Salvadora persiccontain biologalso provide richnins. Therefore

cterial compoundpathogens like us aureus , Staphke Amicasin,

arfloxacin were o different solvene mentioned druto that prepared uller –Hington ay Sequential Soxciated with R –rmed by Agard extracts and entation.

Key Wotance, Unexplor

Mi

Published on 25t

14

ves against Mathogens

ate Pushpa S.,

gmail.com

M. College,Nashi

to designate Fshrubs,palms, epoves.These wetlaand more than

e and other coasmical which alsoe tree resource iswood, timber, ch

microbial activitypecies found aloapiculata, Sonnecennia officinalica, Sesuvium po

gically active ah source of steroit is worth to scds to combat th Streptococcushyllococcus xyluNetilmycin , Gtreated with t

nts (EA, MeOHugs.Extracts prepin Methanol (M

agar by Agar Gelxhlet Extraction

–plasmids.The phrose gel electro

the potentiatio

ords ed, Synergestic E

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Multidrug-Re

, Rahatekar R

ik.,Pune Univers

Facultative haloppiphytes,ground and ecosystems 80% of marine c

stal ecosystems w protect the shors usedfor variouharcoal ,fodder,y of mangroves

ong the east and eratia alba, S. is, A. marina, ortulacastrum. Mantiviral,antibacoids , triterpenescreen mangrovehe pathogenic bs , E.coli , Kleulose , XanthomoGentamycin, Cthe mangrove e

H) in Muller-Hinpared in Ethyl ac

MeOH). Sensitivitl Diffusion Methmethod. Most o

hysical partial oophoresis technon activity with

Effect, Potentiat

Conference on

1st Century February 2011 tract Book

Page 17 

esistant

Rohini R.

sity.

phytic and ferns and

are among catches are worldwide. reline from

us purposes medicinal are largely west coast caseolafis, Bruguiera

Mangroves cterial and s, saponins, e plants for bacterial & ebshiella , onas which

Cefotaxime, extracts at

ngton Broth cetate (EA) ty of MDR hod. Extact of the drug

or complete nique. The h standard

tion Effect

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Progressiv

Modeand CDepa

 

Biosorptio

Chro

bioaccumulati

microorganism

research empl

effects of pH

contact time w

Chromium w

treatment of b

The optimum

and 150 rpm r

the metal as w

initial contact

and Langmuir

10mM NaOH

carboxyl grou

from potentio

the fungal my

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

on of hexavadead fun

Dee

Departmen

15 Ma

omium presents

ion and bioma

ms for removal

loyed dead biom

, temperature, s

were examined.

with a decrease

biomass using 1

temperature and

respectively. Th

well as biomass.

exhibiting 1st o

r Isotherms. De

H at S/L of 2 f

ups of the fungal

metry and FT-IR

celium.

Arts, Science 11005

biology

PP1

lent chromiugal biomass o

ep.V.Barot & V

barot_deep@y

nt of Microbiolog

adam Cama Roa

s threat to th

agnifications he

of Chromium

mass of P. chry

haking speed, m

pH was the mo

in pH. Bios

1M phosphoric a

d shaking speed

he removal of Ch

The biosorption

order kinetics. S

esorption and re

for five biosorp

l cell wall contrib

R analysis. SEM

Mi

Published on 25t

15

um from aqueof P. chrysog

Varsha.Vaidy

yahoo.co.in

gy, Institute Of S

ad Mumbai-4000

he environmen

ence necessitati

by biosorption

ysogenum in bat

metal concentrat

st critical factor

sorption was en

acid coupled wi

for the biosorpt

hromium was af

n reached equilib

Sorption data we

e-usability studie

ption -desorptio

buted to the sorp

M indicated accu

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

eous solutiongenum

ya

Science,

032

nt due to its

ng its remova

is gaining atten

tch experiments

tion, mass of bi

r increasing the r

nhanced from 1

ith autoclaving t

tion were found

ffected by conce

brium after 165

ere described by

es indicated use

n cycles. The

ption process as

umulation of Ch

Conference on

1st Century February 2011 tract Book

Page 18 

ns using

s toxicity,

al. Use of

ntion. This

where the

iomass and

removal of

13.19% by

to 22.93%.

to be 25°C

entration of

min of the

freundlich

efulness of

amine and

confirmed

hromium in

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Modeand CDepa

 

Study o

De S

2Institute o

Campfor both humhuman CampCampylobacteproduction. Ccommonly assthan those causystemic casesas they are sechoice to treatantibiotics inCampylobacteantibiotics belaminoglycosidciprofloxacin,slaughter hou(MIC) as wellthe agar dilutaccording to Institute). Brevalues for azitis also necessplasmid or chaims at stu

Agar D

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

of Antibiotic RIsolat

ouza N. A.1, K

1Departmeof Bioinformatics

pylobacter are tan and animal dpylobacter infecer mainly as a cCampylobacter jsociated with baused by Salmons, most cases of lf-limiting. Mact such infections

ncluding trimether isolates werelonging to the cdes were selecte gentamicin and

uses around Punl as the minimumtion and broth m

breakpoints speakpoints for resthromycin and esary to determinhromosomally mudying the pre

Dilution, Antibiot

Arts, Science 11005

biology

PP1

Resistance Pated from Pun

Kamble A.2, P

bpkap@unipent of Microbiolos and Biotechno

thermophilic Gradisease. Poultryctions. Antimionsequence of tjejuni and Camacterial diarrhoenella and E.colif campylobactericrolides and fluos. Campylobactehoprim, vancom studied for theclasses macrolid

ed for the study. d tetracycline. Thne, Maharashtram bactericidal comicrodilution mepecified by CLsistance to ciproferythromycin arene whether the

mediated by studevalence of an

Ketic Resistance, B

Mi

Published on 25t

16

atterns in Cane, Maharash

arkar S. D.1,

pune.ac.in ogy, University ology, University

am negative enty is one of the mcrobial resistan

the unchecked umpylobacter coliea. C. jejuni acc

combined. Excosis do not requ

oroquinolones arr shows intrinsic

mycin and cefoeir antimicrobialdes, fluoroquino

They included he cultures used a. The minimumoncentration (Methods. The MICLSI (Clinical afloxacin are estae ≥8 µg/ml and resistance to th

dies involving pntibiotic resista

y Words Broth Microdilut

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ampylobacterhtra

Kapadnis B. P

of Pune of Pune, Pune 4

eric pathogens rmost important nce has emerguse of antibioticsi have been focounts for morecept for certain uire antimicrobiare regarded as thc resistance to a operazone. In tl susceptibility polones, cephaloserythromycin, cwere isolated fro

m inhibitory conBC) was determC values were d

and Laboratory ablished at ≥4 µ≥32 µg/ml resp

he antimicrobialplasmid curing. ance in Camp

tion, Campyloba

Conference on

1st Century February 2011 tract Book

Page 19 

r spp.

P.1

411 007

responsible sources of ed among

s in poultry ound to be e infections severe and

al treatment he drugs of number of

this study, pattern. 25 porins and

ceftriaxone, om poultry ncentration

mined using determined

Standards g/ml while

pectively. It l agents is This work

pylobacter.

acter

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Modeand CDepa

 

AntimicrCommonly

Gauri D

1Post gr

2Research Ce

Elimibacteria like Sthe field of mantibiotics speacquired infecsafe drugs toeffective antibpharmacologicsulphanylamidbiologically acto increase thactive groups.SDZ. Our chiagainst some pathological sidentified as Edevelop resistmost commonpump may beassayed by wboth clinical iexhibited a gtrimoxazole w

Multidrug R

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

obial Activityy Occurring M

evasthale1, A

raduate departmA

enter in Chemist

ination of the mStaphylococcus, medicine. These ecially sulphonactions. It is thus fight these nobacterial agentscal activities. de and structurctive Gallic acid

heir antimicrobia. The compoundief aim was to c

of the most csamples. . The E.coli, Staphylotance to sulphonn and basic mece present in the

well diffusion meisolates as well good antibacteri

was used as a stan

Resistant Pathoge

Arts, Science 11005

biology

PP1

y of New SulpMultidrug Re

Alok Vyas2, Di

dgauri1972@yent of Microbiol

Azam Campus, Ctry, Abeda Inamd

Pune-

most widespreadE.coli and Pseustrains have ac

amides. They ares the need of theosocomial pathos for many year

Seven new cally characteriz

d moiety were al spectrum. Th

ds were named acheck the activitommonly occurorganisms wer

ococcus and Psenamides rapidly achanisms of mu

resistant cells. ethod for antimas standard cul

ial activity. MIndard.

Keywoens, Antibacteria

Schiff B

Mi

Published on 25t

17

phanylamideesistant Urin

vya Nair1 and

yahoo.co.in logy, Abeda Inam

Camp, Pune- 1 dar Senior Colle- 1

d multidrug resisdomonas has be

cquired resistance also responsible hour to develogens. Sulphonamrs and are wellcompounds werzed. The sulfonincorporated int

he side chains was STZ, IND, THty of these newrring multidrugre isolated fromeudomonas. It seand efflux pump

ultidrug resistancThe newly syn

microbial activityltures. Among thIC and MBC w

rds: al Activity, STZ

Bases

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

e Analogues anary Tract Pa

d Jhilmil Gho

mdar Senior Col

ege, Azam Camp

stant strains of ecome a major chce to most of thle for many of thop new more effmides have beel known for there synthesiamide- Schiff bto these moleculwere substitutedHB, DHB, CHRly synthesized cresistant patho

m urine sample eems that these ps are considerece. One or mor

nthesized compoy against these he tested compowas also perfo

, Sulphanylamid

Conference on

1st Century February 2011 tract Book

Page 20 

against athogens

oshal1

llege,

us, Camp,

pathogenic hallenge in he existing he hospital fective and en used as eir diverse ized from bases with les in order d with new , SNL, and compounds ogens from

and were organisms

d to be the e than one

ounds were pathogens

ounds STZ rmed. Co-

de, UTI ,

 

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Progressiv

Modeand CDepa

 

Isolation

Bacteria fr

Gauri Paw

Departm

Biolu

specific wavel

Some marine

showing symb

bioluminescen

luminescent d

parameters su

Maximum lum

produced by d

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

n, Identificati

rom Fish Sam

war, Deepali M

ment of Microbiol

uminescence is

lengths are emitt

fishes have evo

biotic associatio

nt bacteria both

diplococci were

uch as temperatu

minescence was

diplococci are ca

Bio

Arts, Science 11005

biology

PP1ion and Char

mple and Its U

Mane, Vyanka

and Smita B

logy, Modern co

Shivajinagar Pu

the light emis

ted due to conve

lved the ability,

on. These anima

a safe place to

e isolated from

ure and pH were

s observed at 2

atalase, oxidase a

Key wo

oluminescence,

Mi

Published on 25t

18 racterization

Use as Water

atesh M. Sana

Bhuyan*

ollege of Arts, Sc

une 411 005

ssion phenomen

ersion of chemica

to harness these

als have special

live and a sour

marine squid.

e observed on pr

260 C and at p

and esterase.

ords

Squid, Esterase

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

of Biolumine

r Pollution In

am, Shilpa Mu

cience and Comm

non in which p

al energy into lig

e light-producing

l light organs th

rce of food. Gra

Effects of env

roduction of lum

ph 8. The enzy

Conference on

1st Century February 2011 tract Book

Page 21 

escent

ndicator

ujumdar

merce,

photons of

ght energy.

g microbes

hat provide

am Positive

vironmental

minescence.

ymes were

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Modeand CDepa

 

Assessmen

1Department o

Inforgermplasm is The genetic diinvestigated pcultivars usinprimers were similarity betwutilized to con(UPGMA). Tsegregation amgene pool orig

 

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

nt of Genetic

Hris

of Biochemistry,

rmation about vital for develo

iversity/relatednpreviously. We

ng randomly amsurveyed, out

ween genotypes nstruct a dendog

The genotypes wmong varieties fogin.

RAPD,

Arts, Science 11005

biology

PP1

c Diversity amMark

shikesh Joshi

New Arts Comm2ICAR, Ela, Old

the extent of oping coherent s

ness among manghave assessed

mplified polymorof which 15 wwas in the range

gram using the uwere grouped infound may be du

Key wo

Germplasm, UP

Mi

Published on 25t

19

mong Mango kers

¹, A. R. Desai

merce and Sciencd Goa, Goa.

genetic diversstrategies for fugo cultivars deve

the genetic divrphic DNA (RA

were selected foe of 0.10 to 0.66%unweighted pair nto two main clue to less geograp

ords

PGMA, Dendogr

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Varieties by

ce College, Ahm

sity/relatedness uture gains in preloped in Goa haversity among

APD). 25 randoor further studie%. These coefficgroup of arithmlusters. But no phic distance an

ram.

Conference on

1st Century February 2011 tract Book

Page 22 

y RAPD

mednagar

in mango roductivity. as not been 16 mango

om ten-mer es. Genetic cients were

metic means significant

nd common

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Progressiv

Modeand CDepa

 

Develop

1. D2. Head

Biostgrowth. Biostitexture,improveffect of pH fertility whimicroorganismbacteria livingmicroorganismuse as biostim

Soil Fertility

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ment of MicrSaline So

Indulkar V.

Department of Md, Department of

timulants is a claimulants enhancve plant ability and soil colloid

ich are nitroms, siderophoreg around root ms as well as sa

mulant to enhance

y, Saline Soil, Sa

Arts, Science 11005

biology

PP2

robial Biostimoils for Susta

.S.* Siddiqui A

Microbiology, Dayf Microbiology,

ass of product wces shoot and roo

to recover fromdal imbalance. Vgen fixing mproducing micr

system. Presentalt tolerant Azotoe fertility of salin

Key Woalt Tolerant Mic

Biostimu

Mi

Published on 25t

20

mulants to Enainable Agric

A.G.1, Ranjek

ayanand Science Dayanand Scien

which enhances sot development,m disease and inVarious microormicroorganisms,roorganisms, fut work is aimedobacter species ne soil.

ords croorganisms, Saulants

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

nhance Fertiulture

kar M.K.2

College, Latur. nce College, Latu

oil fertility as wimprove soil str

nsect damage, rrganisms contrib phosphate

ungi, nematodesd to isolates safrom saline soi

alt Tolerant, Azot

Conference on

1st Century February 2011 tract Book

Page 23 

lity of

ur.

well as plant ructure and reduces the bute to soil solublizing , protozoa, alt tolerant il and their

tobacter,

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Progressiv

Modeand CDepa

 

Assessm

Depa

Moriregions of noAmerica. Its pthe removal oorganic polympolymers and hazards to huma natural,cost water of our materials for dseed was extrMoringa Oleifsludge conditmicroorganism.MOCP was fto verify the cthe potential tcost.

Moringa ole

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ment of WaterCo

Jain Ree

artment of Micro

inga oleifera (Mrth-west India a

pods have been eof organics, andmer. Treatment inorganic chemi

man health. So,theffective,eco fricampus. Moring

drinking water trracted and thenifera coagulant tioning propertiems at room temfound to be stablcoagulation actito be used in th

eifera , Moringa

Arts, Science 11005

biology

PP2

r Purificationoagulant Prot

ena R. and Ka

reenajain512@obiology, Moolji

MO) is a multipuand indigenous temployed as an

d coagulant for plants general

icals which are nhe main objectiviendly adsorbentga oleifera seedreatment. The con purified on thprotein (MOCPes as alum. It amperature. It alle at pH 6.8.A smvity experiment

he wastewater tr

Key Wooleifera Coagula

Mi

Published on 25t

21

n Potential oftein (MOCP)

adam Shradh

@gmail.com i Jaitha College,

urpose, mediumto many parts oinexpensive andwater treatmently uses materianot that much cve of this work wt for the examinds were investigoagulant protein he lab scale by P) possessed conalso showed antso inhibits the mall volume coats. We concludeeatment in an e

ords ant Protein (MO

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

f Moringa Ole)

ha D.

, Jalgaon-42500

m or small-sized of Asia, Africa, d cost effective t. It is a non-toxals like synthetost worthy and a

was to use the Mning the quality ogated as alternatfrom the Moringusing dialysis

nsiderable coagutimicrobial effebacteriophage

agulation method that the MO bi

efficient way and

OCP), Coagulatio

Conference on

1st Century February 2011 tract Book

Page 24 

leifera

02

tree, from and South

sorbent for xic natural tic organic are causing

MO seeds as of drinking tive natural ga Oleifera technique.

ulation and cts against replication d was used iomass has d with low

on, Alum.

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Modeand CDepa

 

A Cost-Effe

Analysi

* Jogi

* Department

Mushproduced abovcultivated andpopular for thwas used anthroughout. Mobserved wasreported but thof the flower nwas 77 g and tflora of mushmainly includincludes K,Mfurther optiminflorescence

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

fective Protoc

is and High Y

i M. M., Surya

Anandwa

t of Microbiology

hroom is a flesve the ground ond analysed the

heir nutritional vd temperature

Mushrooms wers regarding the he count obtainanormally attainethe dry weight whroom sample. des proteins, car

Mn,Cu,Na,Zn,Ca.mization of cond

and weight of th

Mushroom, F

Arts, Science 11005

biology

OP0

col for Cultiv

Yielding Nutr

awanshi Supr

ni Nilesh P., a

mopa2005@red

gy, PSGVPM’s, A

shy, spore-bearin soil or on agrospecies of Mu

alue. For the cuof 18-320C anre harvested on

inflorescence. able in the preseed is within 60-1was 11 g which w

We have biochrbohydrates, Fat, ( AAS and Fl

ditions which ishe fruiting body.

Key woFungiculture, AA

Mi

Published on 25t

06

vation, Micro

ritious Variet

riya V., More B

and Patil Jaye

diffmail.com ASC College Sha

ing, fruiting boo industrial wastushrooms Pleur

ultivation purposnd humidity of n 21st days. AAn average of

ent study was up00 g. The wet w

was quite satisfachemically analy, amino acids anlame Photometes likely to give

ords AS and Flame Ph

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

bial and Bioc

ty of Mushro

Bhagyashree

esh A .

ahada, 425409, N

ody of a fungutes. In our projeotus Sajar-Kajue. Well sterilize80-85% was m

Another promisif 10-12 flowerspto 20 flowers. T

weight reached inctory. We have dsed the mushrond minerals wher ) .We intende better yield in

hotometer

Conference on

1st Century February 2011 tract Book

Page 25 

chemical

ooms.

e V.,

Nandurbar

s typically ct we have

u which is ed substrate maintained ng feature

s has been The weight n this study done micro oom which hich mainly d to go for n terms of

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Progressiv

Modeand CDepa

 

Biotra

P.G. Departm

Relea

toxicity, muta

given in deter

the azo dyes. A

the study of d

2R. In all 12 p

isolated which

medium and

showed decolo

dye was deter

reduced the C

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ansformation

Ka

ravimicro3@g

ment of Microbio

ase of azo dye

agenicity and rec

rmining the abili

Acclimatized mi

decolorization an

promising isolate

h could decolor

up to 68.00 %

orization up to 6

rmined by spect

OD by more tha

Arts, Science 11005

biology

OP0

n of Textile A

ale R. V. and T

gmail.com , prak

ology and ReseaBarshi – 413 4

in the environm

calcitrant nature

ity of microorga

icroorganisms is

nd degradation o

es of Reactive O

ize 1000 μg/ml

in half strength

67.00 % in less th

trophotometer at

an 85.00 %.

Mi

Published on 25t

05

Azo Dyes by A

Thorat, P. R.

kash_micro1@y

arch Center, Shr411, Solapur

ment is of grea

of the dye, cons

anism in decolor

solated from natu

f the dyes React

Orange 16 and 1

of dye to more

h nutrient medi

han 24 hours. Pe

t (λmax) 492 nm

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Aerobic Bacte

yahoo.co.in

i. Shivaji Mahav

at concern due

iderable attentio

rization and degr

ural sources, we

tive Orange 16 a

isolate of Orang

e than 70.00 %

ium. The cell f

ercent decoloriza

m and 484nm. S

Conference on

1st Century February 2011 tract Book

Page 26 

eria

vidyalaya,

to, colour,

on has been

radation of

ere used for

and Orange

ge 2R were

in nutrient

free extract

ation of the

Six isolates

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Progressiv

Modeand CDepa

 

Isolation

Ka

1Instit

Campcause of bacbacterium, copoultry, antibiin live poultrybacterium. PhBacteriophagehas been donepoultry in Punstudy 26 isolBiochemical tconfirm themcombat the prspecific.

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

of Bacteriop

amble A.1, De

tute of Bioinform2Department o

pylobacter jejuncterial enteritis ommonly found iotic resistance iy is difficult. An hage-therapy is es specific for the on it in India yene and their perclates of Campytests were carrie

m. Alongside 6 bresence of Camp

Campylob

Arts, Science 11005

biology

PP2phage against

Pun

Souza N.2, Pa

bpkap@unipmatics and Biotec

of Microbiology

ni is a Gram-negworldwide. It in poultry. Due

is rampant in Caincreasing trenda promising a

his bacterium haet. This study focentage reductioylobacter were ed out along wbacteriophages wpylobacter jejun

Key Wobacter, Bacteriop

Mi

Published on 25t

22

t Campylobacne

arkar S.2, Kap

pune.ac.in chnology, Univery, University of P

gative food-bornis a microaerope to the unrestrampylobacter. Bd in antibiotic realternative for Cave been isolatecuses on isolatio

on of target C. jeisolated from sith a latex agglwere isolated fr

ni which were fo

ords phage, Phage-th

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

cter from Pou

padnis B.P.2*

rsity of Pune. PuPune. Pune

ne pathogen that philic and thericted use of antiocontrol of thissistance is obser

Campylobacter ed previously buon of the phages ejuni strains. In tslaughter houseslutination test anrom Chicken Inound to be Cam

herapy.

Conference on

1st Century February 2011 tract Book

Page 27 

ultry in

une

is a major rmotolerant tibiotics in

s bacterium rved in this biocontrol. ut no work from retail the present s in Pune. nd PCR to ntestines to

mpylobacter

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Modeand CDepa

 

Isolation

D

FreshIndian subconmicrobiologic(AB), SalmoStaphylococcusamples colleindividual samfor future tesmicrobial constudies. Knowprogramme anare necessary,isolates were susceptibility to be resistantwere resistantemergence of measures. Theprovide an altPractices to cooperation iconsultations resistant patho

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

n and Identifi

*Ketki N

Department of M

h and frozen seantinent. Prawns cal contaminatioonella and Shus aureus, Vibrected from fish mples varied widsting of seafoodntamination of wledge of contamnd effective hyg, should contamicollected from ttest for 16 antibt to 50% of the t to at least one

f new pathogens,ese 12 drug resternative to antibensure pathoge

is essential, andto address the

ogens and the po

Arts, Science 11005

biology

PP2

fication of Pat

Nalawade and

Microbiology, The

afood products a(Penaeus mono

on. Total bacteriShigella spp., rio spp and Psemarkets across

dely. The method, and as a temseafood for comminations is ver

giene as well sanination by colifothese experimeniotics from varioantibiotics used

e drug. The inte, and the need fistant strains we

biotics. These stuen free sea food FAO, OIE a

issues related otential public he

Mi

Published on 25t

23

thogens of Se

d Rajendra Ch

e Institute of Scie

are in wide use odon) were examial counts of aeEnterobacteria

eudomonas spp Mumbai. The m

od used for analymplate for region

mparative epidery critical in thenitary proceduresorm and faecal conts which were tous classes. 12 od. It was found ensification of fifor sustainable trere treated with udies will help tod supply. Inteand WHO have

to antimicrobiaealth impact.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

eafood Penae

houre

ence, Mumbai.

and typical of mined for the perobic mesophil

aceae, Eschericwas done for

microbiological ysis could servenal testing scheemiological ande Good Hygienics in food procesoliform bacteria then subjected toof these isolates w

that 98.81% of fish culture has reatments and pr several herbal

to establish Goodernational interde organized a nal use, the eme

Conference on

1st Century February 2011 tract Book

Page 28 

eus sp.

the diet in presence of lic bacteria chia coli,

10 prawn quality of

e as a basis me on the

d statistical c Practices

ssing plants persist. 51

o antibiotic were found the strains caused the rophylactic extracts to d Hygienic

disciplinary number of ergence of

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Modeand CDepa

 

To Study A

Department of

The mliterature. The13 medicinal pand ethanolicmicrobial actidetect the zoGarcinia indicand many comcan prevent thprepared from

Herbal Extra

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Anti-Bacteria

Kh

of Biotechnology

medicinal propee purpose of thisplants and to pre

c extracts of diivity on Blood one of inhibitioca showed maxmmercial toothphe growth of de

m the most effecti

acts, Anti-Bacter

Arts, Science 11005

biology

PP2

al Activity of

Mixed Den

hadpekar A.*,

y, Modern Colleg

erties of several s study is to evalepare a poly herifferent medicinAgar Medium. on. Terminalia

ximum zone of inastes were testeental bacteria anive samples.

Key worial Activity, Ag

Flora

Mi

Published on 25t

24

Various Med

tal Flora

, Thombre R.

ge of Arts, Scienc

plants been docluate the anti-barbal product in thnal plants wereAgar well diffuchebula, Cary

nhibition. The med against mixednd can act as an

ords gar Well Diffusioa.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

dicinal Plants

ce and Commerc

cumented in ancacterial effect of he form of tablee screened for usion method wyophyllus aroma

mixture of effectid dental flora. Tn astringent. Ta

on Method, Mix

Conference on

1st Century February 2011 tract Book

Page 29 

s against

ce, Pune 5.

ient Indian extracts of t. Aqueous their anti-

was used to aticus and ive extracts he mixture

ablets were

ed Dental

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Modeand CDepa

 

S

1 D2Head

Laccfound in whitesource would wheat & maizwoody part ofmedia containthe effect of cfiltrate would

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Screening and

Kawade S

Department of Md, Department of

ase are multi ce rot fungi. Laccbe used in place

ze. A purpose of dead plants. T

ning guaiacol. Scculture filtrate ond be detected.

Lact

Arts, Science 11005

biology

PP2

d Production

S.S.*, Diwan V

Microbiology, Daof Microbiology,

opper oxidases case act on broae of glucose & gof this study is tThese isolate woucreened fungi wn dye decoloriza

Key Wotase, Fungi, Dye

Mi

Published on 25t

25

n of Laccase f

V.A.1, Ranjeka

ayanand ScienceDayanand Scien

having wide suad range of subsguaiacol for laccto isolate fungi uld be screenedould be tested fo

ation. Also chara

ords Decolourization

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

from Fungi

ar M.K.2

College, Latur nce College, Lat

ubstrate specifictrates. The prim

case production tfrom industrial

d for laccase profor production ofacterization of th

n.

Conference on

1st Century February 2011 tract Book

Page 30 

tur

city mainly mary carbon

that is rice, effluent &

oduction on f laccase & hese culture

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Progressiv

Modeand CDepa

 

Studies on

Departm

Provd

Shivajinagar, bioemulsifier broth. Provden

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Bioemulsifie

Lalit Lande, S

ment of Microbio

dentia stuartiiPune. It showeof 237 EU/ml antia stuartii also

Arts, Science 11005

biology

PP2r Production

from Gard

Smita Bhuyan

lalitlande5532@ology, Modern co

Shivajinagar

was isolated ed good emulsifat 300C, PH 7.5 wo showed produ

Mi

Published on 25t

26 n from Provdeden Soil

n and Shilpa

@gmail.com ollege of Arts, scr, Pune 05

from garden sfication activitywith (0.5%) sun

uction of urease

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

entia Stuartii

Mujumdar

cience and comm

soil of Moderny and produced nflower oil in Lu

and gelatinase.

Conference on

1st Century February 2011 tract Book

Page 31 

i Isolated

merce,

n College, maximum

uria Bertani

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Progressiv

Modeand CDepa

 

Compara

1. D

In thbioinoculants for nitrogen fserial dilutionconfirmation oby subjecting bioinoculants Organisms weof the rhizospThe root lengtwith bioinocuincreasing thein soil and alamino acids, sthe environmebe isolated fAlso, such bichemical fertil

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ative Study OSoil A

M

Department of b

his project a prepared from

fixing organism n using Ashby’sof organism. Plathe organism twere prepared o

ere counted by Nphere was more th and the shooulant preparede nitrogen contenso productivity;sugars used by pent giving highfrom the soil aniofertilizers woulizers.

Arts, Science 11005

biology

PP2Of Bioinoculan

And Non-Rhi

M. S. Vrushali2

saivrush.1989@biotechnology, Sp

2. Dr. D. Y. Pati

comparative rhizosphere soifrom rhizosphe agar. Gram sta

ant growth promto phosphate solon ashby’s brothNeubaur’s chamb

active physioloot length of the pd from rhizosphnt of the soil; ph Plant Growth P

plants. As the rhh efficiency in tnd custom-mad

uld not disturb t

Mi

Published on 25t

27 nts Preparedizosphere So

2, N. Rutika1

@yahoo.com picer Memorial il University

study was cil and non-rhizoere and non-rhizaining and Caps

moting rhizospherlubalition using h using suitable ber. Lab trials wogically than thaplants was foundhere. This projehosphate solubilPromoting Rhiz

hizosphere organterms of yield o

de biofertilizers the biological c

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

d From Rhizooil.

College, Pune.

arried out besphere soil. The

zosphere soil wasule staining ware properties wePikovskayas agstrain and kept

were done. The at of non-rhizosd to be more in sect will be applisation increase

zosphere organisnisms are more of crop such orga

can be given cycles as is don

Conference on

1st Century February 2011 tract Book

Page 32 

osphere

etween the e screening as done by as done for re detected gar. Liquid on shaker. microbiota

sphere soil. soil treated plicable in phosphate

sms release adapted to

anisms can to farmers.

ne by other

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Progressiv

Modeand CDepa

 

Isolation of

Mad

Depa

Micrwere isolated Screened for hydroxymate positive .Thissiderophore pbroth. It wasEnvironmentaoptimum for m

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

f Siderophor

dhuri Londhe,

artment of Micro

oorganisms fromon Luria agar

sederophore protype of siderophs organism takeproduction was s found that Kal factors pH 5.maximum sidero

Arts, Science 11005

biology

PP2

re Producing of Adathod

, Smita Bhuya

obiology, Moder

m rhizosphere s. Twelve strain

oduction by Arnhore respectivelye for further stcarried out withing’s B Media .6, Temperature

ophore productio

Mi

Published on 25t

28

Organism frda Vasica

an and Dr.Shi

rn College Shivaj

soil sample of Ans were isolatednow and Caskys y. Gram positivetudy ,Media oph Succinate bro

produces maxe 280C, aerationon.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

rom Rhizosph

ilpa Mujumda

ajinagar, Pune -5

Adathoda vasicad. One Gram po

assay for Catace rod showed Arptimization for

oth, Luria broth,ximum siderophn at 120 rpm w

Conference on

1st Century February 2011 tract Book

Page 33 

here Soil

ar

5.

a (Adulsa) ositive rod cholate and rnow assay maximum

, King’s B hore. Other were found

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Progressiv

Modeand CDepa

 

Comparati

Mahesh R

1Depart

Obesrisk. The synddeveloping coof abnormal areason could strong associperturbations hot topic of recommunity in16s rRNA geclones were sanalysis conficontrol rats th

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ive Analysis o

R Ghule1, Raje

tment of Microbi2National

sity is defined adrome is just no

ountries and Indiand visceral obenot explain thi

iation between were reported, a

esearch. To comn a rat model of ene based librarysequenced for e

firmed that the an diet induced

Arts, Science 11005

biology

PP2of Faecal Mic

Wistar R

esh M ChomaShouch

iology, Modern Centre for Cell

as abnormal fat ot limited to thea is no exception

esity was reporteis obesity epide

gut microbiotaa keen hunt for

mplement these efobesity. In this

y for control aneach of the fouratio of Bacteroobese rats.

Mi

Published on 25t

29 crobiota fromRats.

l1, Deepak R he2

College, ShivajinScience, Pune-4

accumulation the western countrn. Recently, an aed from one of temic. Recent disa and obesity.an exclusively

fforts, we initiatstudy, we have

nd obese wistar ur animals in eaoidetes to Firmi

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

m Normal an

Patil2 and Yog

nagar, Pune-411411007.

hat imposes a gries, it has sprawalarming rate of the metro cities.scoveries have

Although phyresponsible bact

ted screening guconstructed and

r rats. Approximach group. Bioiicutes is higher

Conference on

1st Century February 2011 tract Book

Page 34 

nd Obese

gesh S.

1005

great health wled many prevelance . A genetic revealed a

ylum level terium is a t microbial d screened,

mately, 200 informatics r in gut of

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Progressiv

Modeand CDepa

 

Producti2526)

Ma

The p

xylinum is oncellulose(BC)in release of hNowadays, bahealth care inwhich limits iBC using fruapplication fstandardized iindicate maxim(0.5%). Fruit medium as caCellulose obtamethod. Mix the banana peon the cotton against the con

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

on of BacteriUsing Fruit

nasi karmark

manasi.mkDepartment

population increne of the widely, Although planharmful byproduacterial cellulose

ndustries. Althouits application ouit waste insteafor medical puin synthetic medmum productionwastes (mashed

arbon and nitrogained was quantfruit waste was el. To prove its gauze and chec

ntrol.

Arts, Science 11005

biology

PP3

ial Cellulose Waste and C

kar, Poorva D

[email protected], of Microbiology

ease necessitatesy studied micront are major proucts such as lige finds wide ap

ugh production oon large scale. Inad of synthetic urpose. Carbondium (Hesrin &n of BC obtainedd banana peel angen source for mtified on dry wefound to be mo

application in thcked with respec

Mi

Published on 25t

30

by AcetobactChecking For

abir and Meg

poorva79@gmy, Fergusson coll

s search for newoorganism for thducers of cellulo

gnin which doespplications in paof BC by A. xyln present study media has bee

n and Nitrogen& schramm) for p

d using Fructosend mix fruit wamaximum produeight basis as weore suitable for the health industryct to water abso

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ter Xylinum (Nr Its Applicat

ghana kukarn

mail.com lege Pune

w biopolymers. Ahe production oose, its degradat

s not occur in caaper making, galinum is known

economical proen studied alonn sources werproduction of Be (0.5%) and Yeaste) was substituuction of biomasell as by specifithe production oy, obtained BC worption & vertic

Conference on

1st Century February 2011 tract Book

Page 35 

NCIM tion

i

Acetobacter of bacterial tion results ase of BC. arment and it is costly

oduction of ng with its re initially BC. Results east extract uted in the ss and BC. c chemical of BC than was coated

cal wicking

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Progressiv

Modeand CDepa

 

Aureoba

Dep Aure

explored for itorganism alsoand along wipigments callmausonii, has(Vinay Rale) hto study Aureformation on level to compmedium. The batch- scale parameters watemperature, pfrom shake concomitant rmolasses and spent wash is initial BOD a2100000 mg/interesting. Ouor no pigmenproduction asorganism unde

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

asidium PulluC

Meenakshi D

partment Of Micr

obasidium pulluts ability to prod

o produces differith the pullulanled melanin. Ths been studied inhas exhaustive r

eobasidium pullumolasses and sppare its performamount of biofermenter in c

as optimized acpH, etc. . Also, flask to ferme

reductions in BOspent wash fromobtained which

and COD for belit respectively.ur last objective nts which itselfs well as BOD er UV light for 2

Arts, Science 11005

biology

PP31ulans GrownComparative

Dhar, Nasim K

robiology, Fergu

ulans, a yeast-likduce pullulan, a rent types of enz

n which is extrahis organism ann our laboratoryresearch experienulans with the spent wash at shamance (growth mass as well as

compare with scording to the fthe morphology

enter. The secOD and COD v

m brewery industh is 24000 mg/lieet molasses is o The results ofwas to try obtain

f helps better rand COD redu

20 minutes.

Mi

Published on 25t

on Various FStudies

Kherad, Vinay

usson College, P

ke fungus has bpolysaccharide zymes which haacellular; it alsond its close rely for quite somnce with the orgsole objective oake flask and atand product fo

s pullulan produshake flask, whfavor of this orgy change of the condary objectivvalues of thesestry. The initial Bit and 215000 mobtained which f this study of ning an organismesult in extrace

uction. This is d

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Feedstocks fo

y Rale

Pune 411004

been well-studieof multiple uses

ave different acto produces the ative, Aureobas

me time and one ganism. We undef growth and prbatch-scale ferm

ormation) in stauction is quite hhen the fermenganism viz., agitorganism is obsve was to ma substrates, viz.

BOD and COD fmg/lit respectivel

is 280000 mg/lone so far are

m which produceellular polysacchdone by exposin

Conference on

1st Century February 2011 tract Book

Page 36 

or

ed and s. This tivities

black sidium

of us ertook roduct menter andard high in ntation tation, served anifest ., beet for the ly and lit and

quite es less haride ng the

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Modeand CDepa

 

BombIt is a large siuses. All partmedicinal purasthama, foodleucorrhoea, mdental caries, compounds frmethanol andorganisms liktyphi, and Caantimicrobial be studied furproduction of

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Antimic

*Mo

*Sinhgad Col

bax ceiba belongized, tall, decidus of the tree suc

rpose. It can be d poisoning, parmenorrhagia, ac

tooth ache etcrom stem and ld water. Effectske Proteus, Pseandida spp. Resactivity of plant

rther for drug debioactive compo

Arts, Science 11005

biology

PP3crobial Effect

onika More, *

llege of Science,

gs to the family uous tree, whichch as stem, leavused in treatmealysis, haemoptycne, bleeding pc. Present studyleaves using sols of these extraeudomonas, Stapsults will pave t extracts as welesigning and planounds.

Mi

Published on 25t

32 t of Bombax C

*Gauri Jadhav

Ambegaon (Bk)

Bombacaceae. h has been founves, root, flowerent of diabetes, kysis of pulmonapiles, diarrhoea,y is concentratelvents such as acts were furthphylococcus authe way towardll as its purificatnt tissue culture

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Ceiba

v

), Pune 41.

It found in tropind to have manyrs, and thorns arkidney stone, coary tuberculosis,, dysentery, skied on extractingdiethyl ether, c

her studied on ureus, E. coli, Sds better understion. Purified pr

e techniques for

Conference on

1st Century February 2011 tract Book

Page 37 

ical region. y medicinal re used for onstipation, , influenza, in disease, g bioactive chloroform, pathogenic Salmonella standing of roducts can large scale

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Progressiv

Modeand CDepa

 

Produ

Moksh

AmoIndustrial Micproduction inStreptomyces and was seledifferent mediwas modifiedEffect of diffestudied and PInorganic nitrRNase activityactivity increaselected for Ractivity was detected at 96to RNase actactivity does Purification opurification siwas the first spurification anenzyme was ethey bound to(Superose-12)increased 2.3 f

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

uction and Pu

Streptom

hada Godbole

1Modern Co2Nati

ng the five Stcro-organisms, Nn MGYP (Malvenezualae (NCcted for furtheria components o

d to PG (Peptonerent organic andPeptone was forogen sources rey increased by 4ased by 6.7 tim

RNase productiondetected at 72 hours. DNase a

tivity. Streptomynot require me

of the enzyme since it resulted intep of purificationd enzyme was leluted unbound ao the column. Th) and the final fold at this step o

Arts, Science 11005

biology

PP3urification of

myces venezua

e1, Neelima Ku

ollege, Ganeshkhional Chemical L

treptomyces culNational Chemiclt extract, Glu

CIM-2215) showr optimization son RNase produne, Glucose) med inorganic nitroound to be theesulted in very 4.8 times (270 U

mes (1986U/mg n. The maximum

hours and maactivity was also yces venezualaeetal ions whereshowed that hean loss of RNase aon. Ion exchangeloaded on DEAEand other impurihe enzyme was trecovery was 1of purification.

Mi

Published on 25t

33 f Extracellula

alae (NCIM 2

ulkarni1 and S

hind Road, PuneLaboratory, Pun

ltures from NCcal Laboratory,

ucose, Yeast exwed maximum Rstudies. In optimction was studiedium which is gen sources on R

e most suitable poor RNase ac

U/ml to 1302 Uto 13285U/mg)

m extracellular Raximum intracel

observed but it e produces non e as DNase actat treatment is nactivity. Ammone chromatographE cellulose (anioities were removthen loaded on F0%. The specif

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ar RNases fro

2215)

S. S. Deshmu

e 411053. ne

CIM (National Pune) screened xtract, PeptoneRNase activity (mization studiesed and the MGYsimple and costRNase productio

organic nitrogctivity. In PG m

U/ml) where as th. Hence this me

RNase activity anllular RNase acwas very less asspecific nuclea

tivity requires Mnot a suitable mnium sulphate prhy was used in non exchanger) coved from the enzFPLC gel filtratific activity of th

Conference on

1st Century February 2011 tract Book

Page 38 

om

ukh2

Centre for for RNase

e) medium (270 U/ml) s effect of

YP medium t effective. on was also gen source. medium the

he specific edium was nd specific ctivity was s compared ase. RNase Mn2+ ions. method for recipitation next step of olumn. The zyme since ion column he enzyme

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Modeand CDepa

 

Isolation S

Optimizat

Neha Nai

1

Penicacylases beloacylase proteiconnecting the( 6-APA) corpenicillin’s .PIsolation of 1Hindustan AmicroorganismPenicillin–V serratia marcphysiologicalATUAVP184FAME analysof ATUAVP bacillus spp.,method (CLUproduction ofCultural Conproduction wOptimum inoobserved at 3volume was (2.0%) max. glucose folloPVA producti

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Screening fo

tion of Cultu

on Produc

k1, Avinash S

1Modern college2Nati

cillin-V acylase ong to N-terminins , amidohydroe side chain to thrresponding sideenicillin acylase

10 morphologicAntibiotic pums was carried o

acylase activitcescens, compal characterizati46 was performesis, isolate was 1846 was comp, and a phylogUSTAL X) wif PVA by B.cernditions and Ewere studied inoculum size wa0°C and pH 7.075ml in 250 mPVA producti

owed by sucrosion is achieved

Arts, Science 11005

biology

PP3

or Penicillin-V

ure Condition

ction of Enzy

Sunder1, LaxPund

e of Arts, Scienceional Chemical L

(PVA) is a pharnal nucleophile olase enzymes their B-lactam nue chain. 6- APAe acts as scavengcally different oune, Screeningout by plate assayty. Activity waaratively resistaion of the Ped by a private iidentified as B

pared with all otgenetic tree waith the related reus. FermentatEffect of Medn batch fermes found to be 1

0, after an incubml Erlenmeyer fion is achievede, and Out of in the case of a

Mi

Published on 25t

34

VAcylase Pr

ns, Effect of

yme from B.

mi Nair1, Vaile2

e & Commerce PLaboratory, Pun

rmaceutically im(Ntn)-hydrolase

that cleave peniucleus, releasingA is the buildinger enzyme for porganisms was dg for penicily method. Out oas checked by nt to penicillin

PVA-producing institute, using

B. cereus. The 1ther known 16Sas constructed

taxa. This is tion parametersdia Supplemenentation under 10%. Maximumbation of 24 hrs,flask. Out of ad in the case all used ‘N’ so

ammonium phos

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

roducing Org

Media Cons

Cereus

idehi Dande1

Pune 411 007, ne

mportant enzymee super family,icillin’s at the a

g 6- aminopenicilg block of sem

phenylacylated cdone from soil lin acylase-

of which 1 isolasensitivity of

n. The morpholoGram positiv

16srRNA seque16S rRNA gene

S rRNA gene sequsing Neighbothe first repo

s such as Optimnts for Maxim

shake flask cm enzyme produ, and optimum d

all used ‘C’ souof glycerol folources used (0.sphate.

Conference on

1st Century February 2011 tract Book

Page 39 

ganism,

tituents

& A.V.

. Penicillin Penicillin

amide bond llianic acid

mi synthetic ompounds. sample of producing

ate showed 6-APA to ogical and ve isolate encing and e sequence quences of ur joining

ort on the mization of mum PVA conditions. uction was dispensing urces used llowed by .3%) max.

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Progressiv

Modeand CDepa

 

CharacAntibiotic

Neha S.

Acinepathogen. A cAIIMS7 is muconsists of fweight (20 kbwas phenotypresistance to harbouring thePhenotypic chmethods. In biby SDS PAGEdemonstrated Activity staintype isolate Aof the beta molecular chaweight plasmi

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

cterization of cs of Acinetob

Vora, Shradh

Departme

etobacter baumaclinical isolate fultidrug resistantfour plasmids, o) and two low mpic and biocheβ-lactam antibioe high molecula

haracterization oiochemical charE followed by acpresence of caring showed that

AIIMS 7 (19.31klactamase (39.4

aracterisation of id is under study

Acinetobacter,

Arts, Science 11005

biology

PP3

Plasmid Encbacter Bauma

ha B. Nadhe, RParde

karishma@unent of Microbiolo

annii is an oppfrom All India It showing high rone high molec

molecular weightemical characterotics. A transfoar weight plasmif β-lactamases wacterization molctivity staining wrbapenemase andt of the two typ

kD and 39.44kD44kD) which af the beta lactamy.

Key Wo

Multidrug Resis

Mi

Published on 25t

35

coded Resistaannii AIIMS

Riddhi J. Shaesi *

nipune.ac.in ogy, University o

portunistic nosocInstitute of Medresistance to β-lular weight (57t (<10 kb each).rization of the rmant Acinetobaid was obtained was performed blecular weight ofwith nitrocefin. Pd AmpC type ofpes of beta lacta

D), the transformappears to be p

mase gene/s prese

ords

stant, Plasmid, β

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ance To β-LaS7, A Clinical

h and Karishm

of Pune

comial multidrudical Sciences, Nlactam antibiotic7 kb), one mid The objective ofHMW plasmid

acter baylyi ADby natural trans

by using variousf β-lactamase wa

Phenotypic charaf β-lactamase inamases present imant T17 showeplasmid encodeent on this high

β-lactamase.

Conference on

1st Century February 2011 tract Book

Page 40 

actam l Isolate

ma R.

ug resistant New Delhi, cs. AIIMS7

molecular f this study d encoded

DP1 (T17), sformation. disc assay as detected acterization n AIIMS7. in the wild d only one

ed. Further h molecular

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Progressiv

Modeand CDepa

 

Optimiz

Neelim

Modern

34 yefruits, fermenextract Peptonand best ethansingle parametemperature aproduced maxnitrogen sourincubation for10.659 g/L reof optimized m10.659 g/L oEthanol was psource, 10 g/fermentation from 6.12 g/Las estimated bPG-2, SCJ-3 acharacterizatioTorulaspora d

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

zation of Gro

a Kulkarni*,

College of Arts,

east cultures wernted foods, sugane (GYP) mediunol producing cetric studies. Efand fermentationximally using 20ce at pH-4, temr BG-1 and 24 hospectively as estmedium for SC

of medium consproduced maxim/L urea as nitrtime. During o

L to 11.2 g/L whby Gas chromatand BG-1 were on and identifdelbreucki respec

Arts, Science 11005

biology

PP3

owth Paramet

Yeas

Shreyas KumHussain Kho

Science and Co

re isolated fromar syrups and um. All the isolacultures BG-1, Sffect of differenn time on ethan0 g/L sucrose asmperature 30 °Cours for SCJ-3 wtimated by Gas J-3 was estimat

sumed. This givmally for culture

ogen source, ptimization stud

hen estimated bytography. The thcharacterized by

fied as Kodamctively.

Mi

Published on 25t

36

ters for Etha

sts

mbhare, Panduokhawala

mmerce, Ganesh

m different sugar agricultural wasates were screenSCJ-3 and PG-2nt carbon sourcnol production s carbon source C, inoculum sizwith ethanol conchromatographyed and the yieldves 92.64 % efPG-2 using 20 gpH-5, temperatdies ethanol cony CAN method ahree best Ethanoy morphology, b

maea ohmeri,

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

anol Producti

urang Lahare

hkhind, Pune 41

containing foodstes using Glucned for ethanol 2 were selected ces, nitrogen sowas studied. Et, 10 g/L cocon

ze 5 % (v/v) inncentration of 7.y. The total carbd of ethanol profficiency of ethag/L fructose as tture 37 °C andncentration wasand 2.242 g/L tool producing yeabiochemical andCandida interm

Conference on

1st Century February 2011 tract Book

Page 41 

ion by

e and

1053.

d materials, cose Yeast production for further

ources, pH, thanol was

nut meal as n 58 hours 77 g/L and

bon content oduced was anol yield. the Carbon

d 48 hours s increased o 5.180 g/L ast isolates

d molecular media and

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Progressiv

Modeand CDepa

 

CompaGlorios

Dep

Glorithe alkaloids fof gout and rmicro organisThus, taking irespect, an atpotential of thmethanol and Escherichia cPseudomonas clinical isolatKlebsiella, Psactivity againformulate a nbased on syneMethanolic exand inhibited gThe highest screening of ecurrent investiis the first reporganism by t(Kalhari).

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

arative in-vitrsa Superba ag

Clinical

Nikita L

partment of Mic

iosa superba (fafrom the plant (Crheumatism. Thsms has stimulainto account thettempt was madhis plant. Extrac

their antimicrobcoli, Staphyloco aeruginosa, Prtes of Eschericseudomonas and

nst cephalosporinew, cost effectiergistic activity oxtract was foungrowth of the cesynergism rate extract showed igation leads to port in vitro whthe extracts of m

Arts, Science 11005

biology

PP3

ro Antimicrogainst LaboraIsolates of C

L. Lad and Ra

crobiology, Instit

amily: Liliaceae)Colchicine and Ge increasing preated the explorae medicinal impode in the currect of dried leavebial activity wasoccus aureus, Sroteus vulgaris, chia coli (3 std Acinetobactern resistant orgaive antimicrobiaof cefotaxime wid to act synergi

ephalosporin resiwas attained apresence of alkfresh sources ofhich revealed symedicinally imp

Mi

Published on 25t

37

obial Activityatory and Anommon Path

ajendra G. Ch

tute of Science, M

) is widely usedGloriosine) are bevalence of muation of alternatortance of Glorint study to inv

es and tubers was determined aSalmonella typhBacillus sp .andtrains), Proteusr. The extract aanisms. The objal combination ith methanolic existically with thistant organisms against Proteus kaloids, flavonoif new antimicrobynergistic inhibiportant ornament

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

y of the Extrantibiotic Resishogens

oure

Mumbai 400032

d as a medicinalbeing used in theltidrug resistantte antimicrobialiosa superba Linvestigate the anas extracted in eagainst standard hi, Klebsiella pd 8 cephalosporis mirabilis, Enalso showed anject of this stufor drug-resistanxtract of Glorios

he cephalosporinin presence of cmirabilis. Phy

ids and steroids.bial regimes in fition of antibiotital plant Glorios

Conference on

1st Century February 2011 tract Book

Page 42 

act of stant

l plant, and e treatment t strains of regimens. nn., in this

ntimicrobial ethanol and cultures of

pneumonia, in resistant terobacter,

ntimicrobial udy was to nt diseases sa superba. n antibiotic cefotaxime. ytochemical . Thus, the future. This ic resistant sa superba

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Progressiv

Modeand CDepa

 

Ext

Niruta Y. K

1Modern col AmoIndustrial Micactivity (257Uextract 0.3%,optimization sand Yeast extrwas useful in(Beef extract and inorganicRNase activitnitrogen sourcmaximum RNfold increase profile of Strpextracellular Rproduced in 92081) produccould be deteenzyme showsuitable methchromatographpH 7.0. Enzym0.5M salt resuloaded on FPL

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

tracellular RNStrep

Killedar1*, An

llege, Ganeshkhi

ng five Streptocroorganisms) S

U/ml) during scr Peptone 0.5%studies first efferact when omitte

n reducing cost o0.8%, Glucose 1

c nitrogen sourcty than inorganice for RNase pro

Nase activity at pin RNase activ

ptomyces sp.(NCRNase was prod

96 hrs. with 76%ced nonspecific ected in absencewed that heat trhods which rehy the enzyme cme bound to CMulting in 3 fold LC gel filtration

Arts, Science 11005

biology

PP3

Nase producteptomyces sp.

nupama Sonaw

ind, Pune 41105

omyces culture Streptomyces spreening in MGY) medium so sct of various meed from MGYP mof the medium. 1%) medium insces showed that ic nitrogen souroduction. Effect pH 6.0 in BG mity and 6.6 fold

CIM 2081) cultuduced in 72 hrs

% sugar utilized wnuclease produ

e of metal ions, reatment and Asulted in verycame unbound wM-cellulose colupurification andcolumn (Supero

Mi

Published on 25t

38

tion and puri(NCIM 2081

wane1 and Su

53. 2National Ch

from NCIM .(NCIM 2081)

YP(Malt extract selected for optedia componentsmedium higher aThe organism

stead of MGYP organic nitroge

rces. Beef extraof initial pH on

medium. Optimizd increase in spure showed that s. and maximumwithin 72-96 hrsucing DNase ati

but Mn+2 ion fAmmonium sulphy poor recoverywhen loaded on Dumn at pH 5.0 ad 16.6% recoveryose 12) resulting

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ification from1)

umedh S.Des

hemical Laborato

( National Colshowed maximu0.5%, Glucose

timization studies was studied. Mactivity was obtacould grow in smedium. Effect

en sources prodact was the moRNase producti

zation studies repecific activity.

in BG medium m intracellular Rs. Streptomyces ivity also. RNafor DNase. Purihate precipitatioy. During ion DEAE-celluloseand eluted with y. CM eluted enin 8.9% recover

Conference on

1st Century February 2011 tract Book

Page 43 

m

hmukh2

ory, Pune

llection of um RNase 1%, Yeast es. During

Malt extract ained. This simple BG of organic

duced more ost suitable ion showed esulted in 2 Production maximum

RNase was sp. (NCIM

ase activity ification of on are not

exchange e column at buffer and

nzyme was ry.

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Progressiv

Modeand CDepa

 

Isolati

P.S, Pa

Kidnworld. Most osalt found in containing elemetabolize diurine, formingby gastrointebacteria were of morphologbelongs to genof different oFTIR analysicoAtransferasTitrimetric anto be around 4

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ion and Ch

atil , Patil Sha

ney/ urinary tracof the stone are c

variety of foodevated amount oetary oxalate. O

g insoluble calciustinal tract micisolated from di

gical and biochemnus- Stomatococoxalate sources. s, which suggee by the organisalysis with KMn

40%.

Arts, Science 11005

biology

PP3aracterizat

Organ

am A , Bedse,

K.T. H. M. Coll

t stone disease composed of oxad. Increased absof oxalate is tak

Oxalate in humanum oxalate and ecroorganism etcfferent sources. mical observatioccus . This organ

Oxaltate degraest the productiosm. The amount nO4. The degrad

Mi

Published on 25t

39 tion of Oxalnism

,Tanuja D, Ja

lege, Nashik

is a major healtalate or calciumsorption of oxalken. Humans lans can be eliminexcretion in faecc. Oxalate degrOrganisms were

ons. Among thenism is further cadation product,on of oxalate dof oxalate degraation rate of oxa

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

late Degrad

adhav Sandip

th problem throm oxalate. Oxalic

late mayoccure ack the enzyme nated through exces and oxalate drading facultative characterized ose organism one

characterized forformate was d

degrading enzymadation was detealate by the isola

Conference on

1st Century February 2011 tract Book

Page 44 

ding

p R .

ughout the c acid or its

after food needed to

xcretion in degradation ve aerobic on the basis e organism r utilization detected by me oxalyl-ermined by ate is found

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Progressiv

Modeand CDepa

 

Comparamarces

Departme

Prodi

reported charaanticancer actmedium to simproduction waair and rhizoswas selected fSerratia marcenvironmentaland purificatiosupplemented pigment than medium whenused as colora

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ative Study oscens Using V

Pankaj .

ent of MicrobiolShivaji

igiosin producedacteristics of havtivity. From an imultaneously enas the aim of thiphere and screefor further studiecescens by API l parameters usion protocol was

with fatty aciddefined media

n compared withants in textile and

Prod

Arts, Science 11005

biology

OP1on The ProduVarious Crud

Applica

.S. Picha & Sh

pankaj.picha@logy, Modern Coinagar, Pune-411

d by Serratia maving antifungal, industrial point

nhance the growtis work. Pigmenned for their abies. It was biochekit. Production

ing crude and palso modified an

d containing soa. Crude carbonh pure carbon soud paint formulati

Key Wo

digiosin, Serratia

Mi

Published on 25t

13 uction of Prodde Fatty Acidations

heetal .P. Par

@gmail.com      ollege of Arts, Sc1005, Maharash

arcescens is a primmunosuppresof view the nec

th of Serratia mnt producing orgility to produce emically charact

n was optimizedpure carbon sournd developed to

ource were founn sources reducurce and the pigions and also for

ords a, Pigment, Pain

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

digiosin by Sed Sources and

rdeshi

cience and Commhtra.

romising drug ossive, antiprolifecessity to obtainarcescens and th

ganisms were isopigment. One is

terized and identd with respect trces. Extraction

o recover pigmennd to give bettece the cost of gment thus obtair therapeutic app

nt.

Conference on

1st Century February 2011 tract Book

Page 45 

erratia d Its

merce,

owing to its erative and

n a suitable he pigment olated from solate, PP1 tified to be to different procedure

nt. Medium er yield of production

ined can be plications.

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Progressiv

Modeand CDepa

 

O

Poojari P

D Acineserious therapof these organof these organdiversity of anfrom various assays and alschromosomal GenElute Bacprimers specifClass D), amishowed positifor Class C ßADC type of ßto be more preThe resistanceare highly usepresent on porganisms. Hview.

A

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Oligotyping oClinical

ritika, Ratnak

Department of Mi

etobacter spp. hpeutic failure. Ranisms have allownisms in the hospntibiotic resistanhospitals in In

so harbored multor plasmid enc

cterial Genomic fic for differentnoglycosides anve amplification

ß-lactamase folloß-lactamase wasevalent among ae of Acinetobacted as a treatmelasmids, it willence this study

Acinetobacter, ß-

Arts, Science 11005

biology

PP4of Antibiotic l Isolates of A

karan Neena,Pardesi Ka

karishma@un

icrobiology, Uni

has emerged as iapid genotypingwed a better apprpital environmennce in twenty-sindia, which shotiple plasmids. Tcoded. GenomicDNA kit. PCR

t classes of ß-lacnd quinolones. On with more thanowed by Class As found to be moaminoglycosides ter to quinolonent now-a-days. l lead to transwill prove to b

Key Wo-lactamases, Am

Mi

Published on 25t

40 Resistance G

Acinetobacter

, Mehta Khyatarishma*

nipune.ac.in iversity of Pune,

important nosocog methods for threciation of the

nt. In the presentix isolates of Ac

owed multidrug The genes for thec DNA was isol

amplification wctamases (Class

Out of twenty-sixn one primer. MaA, Class D andore prevalent. Siand gyrA, parCs is of prime imIf the genes fofer of these gebe important in

ords minoglycosides an

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Genes from r Spp.

ti, Shirode Ab

Pune 411 007

omial pathogense identification epidemiology ant study, we characinetobacter sppresistance by p

e drug resistancelated using Sigm

was done using 3s A, Class B, Cx isolates characaximum isolates

d Class B. The Aimilarly aph-A6

C, QnrA among qmportance since

r quinolone resenes to other

n epidemiologica

nd Quinolones

Conference on

1st Century February 2011 tract Book

Page 46 

bhinav,

s causing a and typing nd survival acterize the p. obtained phenotypic e are either ma-Aldrich 30 pairs of lass C and

cterized, all s had genes AmpC and was found

quinolones. quinolones istance are susceptible al point of

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Progressiv

Modeand CDepa

 

Optimi

Depart

Four agar from rhiNagar, Pune 0further study Three differenmedia 2 were these three mparameter likeand F2 showe320C respectivisolates.

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ization of Am

Pradnya N. tment of Microbi

different Amylaizosphere soil o05, after primary(F1 & F2) whicnt media viz. schecked for the

medium sabourae pH and tempered optimum provely. Time cours

Arts, Science 11005

biology

PP4mylase, Produ

Rhizosphe

Nadivkar andiology, Modern C

ase producing oof Botanical gary screening on stch were showinsabouraud, Amyeir ability to suppaud showed marature were optiduction of amylse of amylase pr

Mi

Published on 25t

41 uction by Funere Soil

d Sheetal. P. College, Shivajin

rganisms were irden of Moderntarch agar plate.

ng zone of clearylase specific mport maximum a

aximum productimized using sablase at pH 7 androduction was al

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ngi Isolated f

Pardeshi nagar, Pune 411

isolated on potatn College of AC

Two fungi werrance on starch

media 1, Amylaamylase producttion of amylasebouraud. Fungald 6, temperaturelso determined f

Conference on

1st Century February 2011 tract Book

Page 47 

from

1005

to dextrose CS, Shivaji re taken for agar plate. se specific tion, out of e. Physical l strains F1 e 280C and for both the

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Progressiv

Modeand CDepa

 

Redu

P

Rapid

wastewater inconsumers. Swastewater trpollutants, whcharacteristicsalso on the efhealth organizto heavy metareduces the edirectly or inddisorders suchheart disease, depletion of thheavy metals bioremediationsludge to redcontained 4.2obtained fromin 24 hours. Senvironmentalincubation timat ambient teconcentration copper from d

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

uction of heav

Prajakta Bhag

Department o

d population grn agriculture. Waludge is compo

reatment processhich usually conss of sludge depefficiency of its trzation (WHO) esal contaminationefficacy of meddirectly linked toh as arthritis, asarterial sclerosi

he body's immucan be reduce

n of domestic duce heavy met1- 6.43 mg/l co

m sludge, four isotudies on reductl factors such as

me. One of the isemperature (28±

and incubationdomestic sludge.

Arts, Science 11005

biology

PP4vy metals from

Microorg

gwat, Priya B

bendrepriya61@of Microbiology,

rowth, urbanizatastewater poses osed of by-prods. It contains bosist of heavy metend on the origireatment, followstimates that 80%n, as it produce

dical treatments o many complicasthma, chronic is, multiple scle

une system. It haed by using bacsludge to reduc

tals content wasopper and 6.0-8olates could remtion of copper frs temperature, polates, R, showe

±2oc), pH 7, 1%n for 28 hours,

Mi

Published on 25t

42 m domestic s

ganisms

Bendre and Vr

@gmail.com Fergusson Coll

tion has increascertain environ

ducts collected oth compounds tals, organic polinal pollution lo

wed by the treatm% of all chronic es free radicals.

by up to 60%ations such as hefatigue syndrom

erosis, Parkinsonas been reportedcteria and fungce its toxicity. s studied. Sludg.15mg/l zinc. A

move up to 54.76rom sludge were H, carbon sourced optimum redu% glucose conproving to be u

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

sewage by usi

rushali Patil

lege, Pune

sed the need fonmental and heal

at different staof agricultural

llutants and pathoad of the wastement of sludge. disorders can bePresence of hea. Heavy metal eadaches, anger

me, diabetes, fibn's disease, ulced that the conce

gi such as AspeHence, bioremege was neutral

Among the twen6% copper and 2 carried out with

ce and nitrogen uction of copper ncentration, 0.1%useful in the re

Conference on

1st Century February 2011 tract Book

Page 48 

ing

or reuse of lth risks to ges of the value and

ogens. The ewater and The World e attributed avy metals toxicity is etc. and to

bromyalgia, ers etc. and entration of ergillus for ediation of

in nature, nty isolates 2.11% zinc h respect to source and up to 71%

% peptone eduction of

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Progressiv

Modeand CDepa

 

IsolatProducing

Priya

Departm

1 g osterile saline. was spread oncolonies wermorphologicastaining and twithout tryptoproduction. AIAA productioThe effect of checked by Ptryptophan (1Absorbance wincubation byby using trypMaximum IApH 8. P2 wastest.

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ion and Charg Organism fr

anka Mane, S

ment of Microbio

of soil sample coSerial dilutions

n sterile Luria –Bre selected anl features ,Gramthen for IAA prophan. Red colo

All showed IAA on than others. Cf time course anP2 and P4 isolat

mg/ml). Uninowas checked at y replacing carboptone as nitrogeA production w

s identified as M

Arts, Science 11005

biology

PP4racterizationrom Rhizosp

Smita Bhuyan

ology, Modern C

ollected from rh of the sample

Bertanni agar pland screened fom character ,motiroduction by Salour formation wproduction. Out

Cultures were prnd carbon and ntes by Salkovasoculated LB bro540 nm. Maxim

on source (yeasten source. Effe

was seen at 37°CM. lacunata and

Mi

Published on 25t

43 n of Indole Acphere of Gram

n and Dr. Shil

College, Shivajina

hizosphere of grawere prepared aates. After incubor preliminary ility, oxidase teslkovaski’s test. M

was considered pt of 8, P2 and Preserved on LB nitrogen sourceski’s test. Mediaoth with tryptopmum IAA was t extract) from Lct of temperatu

C for P2 isolate P4 as unidentifi

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

cetic Acid (IAm (Cicer Arie

lpa Mujumda

agar, Pune – 05.

am was mixed iand 0.1 ml of thbation at 30⁰C fo

characteristics st, catalase test aMedia used waspositive evidencP4 isolates gave agar slants for fs on IAA produa used was LB phan was kept

produced at 72LB broth with sure and pH waand 32°C for P4

fied organism by

Conference on

1st Century February 2011 tract Book

Page 49 

AA) entinum)

r*

.

n 10 ml of his dilution or 24 hrs ,8

by their and capsule s LB broth ce for IAA

maximum further use. uction was broth with as control. 2 hours of ucrose and s checked. 4 isolate at y mini API

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Progressiv

Modeand CDepa

 

Heali

doctors; this pbacteria. Henevidence that an alternate fpotency of cohoney againstthe above honwas found thaspores. All the10% v/v concrest. Candida sugar solutionproperties of hfound that all sugar solutioncontent that aagainst the labof the same omethod. It waThe effect of hinfected burnscolorimetric mhoney was slFurthermore tallantoic memfound that hona negative conof the factors t 

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Wound

Purnim

Department o

ing of infected problem has recence new approahoney helps in a

form of treatmeommon honeys ft S. aureus, P. aneys was carried at Khadi honey we honeys were thcentration while persisted beyond

n with concentrathoney were solethe four organis

n indicating thaaccount for its loboratory strain oorganism were as observed thathoney and SSD s) on preformed measurement of lightly more efto test the effe

mbrane assay waney accelerated tntrol and a salinethat make honey

Arts, Science 11005

biology

PP4

d Healing Pro

ma Ranawat

of Microbiology,

and chronic wently escalated daches for treatinaccelerating wouent. A study wafound in the marerugenosa, Strepout using agar dwas most potenthe most effectivit took a conce

d 30% v/v concetion of sugars cloely due to the ossms mentioned sat honey has soower MIC’s. Sinf Pseudomonas tested for their

t honey inhibitedointment (a combiofilms of S. abiofilms using c

ffective than SSct of honey ons performed usinthe rate of angioe control. This sy more effective

Mi

Published on 25t

44

operties of ho

and Jyoti Ma

Sophia College

wounds is a comdue to the persistng wounds are und healing and tas conducted to rket namely- Daptococci and Cadilution method t and had the lea

ve against pseudoentration of at leentration of honose to that in honsmotic effect of survived beyondome properties nce honey was faeruginosa, four

r sensitivity to hd these strains a

mmon antibiotic cureus and P. aer

crystal violet. It SD on biofilms n wound healingng fertilized Legogenesis as compsuggests that angin wound healin

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

oney

ntri

e, Mumbai.

mmon challengetence of antibiot

required. Therthus should be loevaluate the an

abur, Pondaghat andida albicans.(range 5%-30% ast amount of coomonas, knockineast 25% to kno

ney. A control Mney was set up t

f the sugars presd 30% concentraother than its hfound to be mosr antibiotic resishoney using agat a concentratiocream used on wruginosa was evwas seen that 5of the above

g, angiogenesis ghorn chicken egpared to a positigiogenesis may ang.

Conference on

1st Century February 2011 tract Book

Page 50 

e faced by tic resistant re is some ooked at as ntibacterial and Khadi

. MIC with v/v) and it

ontaminant ng it out at ock out the

MIC using a to see if the sent. It was ation of the high sugar st effective tant strains

gar dilution on of 15%. wounds and valuated by 0% diluted organisms. by chorio

ggs. It was ive control, also be one

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Progressiv

Modeand CDepa

 

Optimi

D The

biotechnologicdiscover and dneed of time from stagnantisolate was isindicated by revealed glucsource and RTshaking condoptimized parrecovery of supernatant oprecipitation acharacterizatioThe enzyme wstimulated enzgood organic to be effectiveexhibited goodthe best subscommercial dImmobilizatioactivity was slipase was foudetergent. Alsdegraded the days.

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ization and CSelected

Rahu

Department of M

growing interecal applications develop new comthe present wo

t fish runoff wasolated on Victzone of hydrolose, ammoniumT (28⁰C ± 2⁰C)ition for favorarameters and feculture medium

obtained by cenand dialysis. Furon of lipase. Optwas found stablzyme activity upsolvent enhancine stimulator for d stability with bstrate, among tdetergent which

on of lipase was studied where 2und efficient in cso the enzyme fat completely.

Arts, Science 11005

biology

PP4Characterizat

Bacillus sp. a

ul Brid and Ra

Microbiology, The

est in lipase that the enzyme

mmercial microbrk deals with liater and its chatoria Blue Olivelysis of olive o

m chloride, olive) as optimum temable lipase prodermentation studm for extractionntrifugation wasrther, cell free sutimum enzyme ale in wide rangep to 493.33%. 1ng the lipase actlipase activity

bleach i.e. Sodiuthose tested. Th was even mcarried in differ% agarose had

complete removawas found to hThe lipase hydr

Mi

Published on 25t

45 tion of Lipaseand Its Appli

ajendra Chou

e Institute Of Sci

production is e present and sobial sources for liipase productionaracterization ane Oil Agar wheoil. Optimizatione oil, potassiummperature with

duction. Productdies revealed thn and purificats further purifieupernatant was uactivity was obsee of pH and tem1% iso- propyl ativity up to 166.6increasing it up

um HypochloriteThe lipase activmore enhancedrent carriers by ethe highest spec

al of olive oil frohave antagonistirolyzed castor o

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

e Production ication

ure

ience, Mumbai

related to theo there is immenipase productionn Bacillus specind application were lipase produn of medium c

m chloride as anoptimum pH of

tion was carriedhat 50 hours wation of lipase. ed by ammoniuused as enzyme erved at pH-7.0 mperature. Potaalcohol was fou66%. Tween-80 to 173.33%. Th

e. Olive oil was fvity was foundd in presence entrapment and cific activity. Thom cotton fabric ic effect on beeoil more than 89

Conference on

1st Century February 2011 tract Book

Page 51 

by a

e potential nse need to n. With this ies isolated wherein the uction was omponents n optimum f 7.0 under d out with as best for

Cell free um sulfate source for and RT0C. ssium ions

und to be a was found

he enzyme found to be

d stable in of CaCl2.

its specific he purified along with

ef fat as it 9.28% in 6

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Progressiv

Modeand CDepa

 

Laboratory

Depa

Polluthreatened hepollutants. BeSurfactants ihydrocarbons.show limitedbiodegradableTherefore in tand asses its contaminated isolate was semulsificationcarbon sourceThe selected iwith glucose. using Acetoncontent usingcomparative acarried out. 6comparison wobserved usinabout greater (Tween- 80). surfactant in b

B

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

y Study on Bi

Ra

artment of Micro

ution caused byalth and sustain

eing water insoluincrease the a. However chemd efficiency. Oe, less toxic, canthe present studyrole in the biowith crude oil aselected on then assay. It was ses (sucrose, glucsolate was foundThe biosurfact

ne precipitation g Folin Loweryanalysis of purifi61.53% biodegrawith 55% for g whole biosurfareduction in theThus, this study

bioremediation.

Biosurfactant, Di

Arts, Science 11005

biology

PP4ioremediatio

from a G

khi Tari and S

obiology, The Ins

y the release nability of the euble, diesel oil aaqueous solubil

mical surfactants On the whole n be prepared ey an attempt waoremediation of and it’s by produe basis of its screened for thecose and lactosed to be Pseudomtant was extractmethod and an

y and Phenol ied biosurfactantadtion was founTween- 80 wh

factant producer.e surface tension

y revealed the ef

Key Wo

iesel oil, Chemic

Mi

Published on 25t

46 n of Diesel O

Garage

Shilpa Sabnis

stitute of Science

of petroleum henvironment. Dalso shows low lity and consare relatively tox

biosurfactants easily and showas made to isolat

diesel oil. On ucts, six isolates emulsification

e better emulsifie) using the sammonas which gavted from cell frnalyzed for its Sulphuric acid

t with chemical sd in presence o

hereas only 42. The biosurfactan in comparisonfficacy of biosur

ords cal Surfactant, B

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Oil Contamin

s

e, Mumbai 40003

hydrocarbons hDiesel is one of

and slow biodegequently degraxic, less biodegr

are specific w widespread apte a biosurfactanscreening garagwere obtained aactivity measurcation activity i

me oil emulsificave maximum emuree broth of Pse

protein and camethod respe

surfactant (Tweeof purified biosu.85% biodegradant was also founn with chemicalrfactants over th

Bioremediation

Conference on

1st Century February 2011 tract Book

Page 52 

ated Soil

32

has always the major

gradability. adation of radable and in action,

pplicability. nt producer ge samples and the best red by oil in different ation assay. ulsification eudomonas arbohydrate ectively. A en- 80) was urfactant in dation was nd to bring l surfactant

he synthetic

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Progressiv

Modeand CDepa

 

Ravindr

Departm

Gibb various develproduction of prime importaPresent work paddy field saproducing cullike pH and te

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Production

ra Baviskar, S

ment of Microbio

berellin (GA) islopmental procef gibberellin andance and it mafocuses on prod

amples. Spectrostures in Czapek

emperature and id

Gi

Arts, Science 11005

biology

PP4n of Gibberell

Sunny GaikwaBhuya

*smitabhuyan@

logy Modern colShivajinagar Pu

a plant hormo

esses in plants. Cd its enhancemenay also meet theduction of gibberscopic analysis rDox broth at 29dentification of t

Keibberellin, hormo

Mi

Published on 25t

47 lic Acid by So

ad, Shilpa Muan*

@gmail.com llege of Arts, Sciune 411 005

one that regulateConsidering its wnt by sources like ever increasinrellin by four direvealed four pr90C for 7 days. Othe fungal cultur

y Words one, Fungi, Czap

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

oil Fungi

ujumdar and S

ience and Comm

es growth and wide array of apke fungi and bacng demand of gfferent fungi iso

romising fungal Optimization of res is in process.

pek Dox

Conference on

1st Century February 2011 tract Book

Page 53 

Smita

merce,

influences pplications, cteria is of gibberellin. olated from gibberellin parameters .

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Progressiv

Modeand CDepa

 

Prevalenc

Dep

Staphpart of humancommon causmortality rategolden periodstrains. Severaa semi synthStaphylococcunosocomial pareplaced by epidemiologicand thereforePrevalence ofand environmsurveillance iMicrobiology months were were also isolresistance. Ousamples 106 29.4%) were 2.8% were MstaphylococciCoNS.

MR

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ce of MethiciPatil Hospit

Reen

partment of Micr

hylococci are ubn flora. The mostse of bacterial e from staphylod was soon interal different classhetic penicillin us aureus was athogen worldw

a more stablcal pattern and ie very difficult f multidrug resi

ment can be a cis important as

laboratory of Dstudied for Me

lated from hospiut of 150 Meth(70.6%) were iMR-CoNS. Am

MR-CoNS. Fromwere isolated, o

RSA, Clinical sam

Arts, Science 11005

biology

OP0illin Resistantal and Resea

na A. Dighe an

drmrsdighe@redrobiology D. Y. P

biquitous in naturt virulent of the infection. Afterococcal infectiorrupted by the apses of antibiotics

was introducereported in 19

wide, popularly de drug Oxacilis well known to

to treat, especstant Staphylococause of outbres a preventive Dr. D.Y.Patil hethicillin resistanital personnel anhicillin resistantdentified as Sta

mongst the hospm the air samout of which, 21

Key Womples, Hospital p

Mi

Published on 25t

02 t Staphylococ

arch Centre, K

nd Saral Ghos

diffmail.com Patil Medical Co

re with about a genus, Staphylo

r the discovery ons dropped drppearance of pes were developeded in 1959. F61 in UK. Sin

designated as MRllin. Staphylocoo develop resistcially those conocci amongst peak of infection

measure. Clinhospital. Kolhapnt staphylococc

nd environment at staphylococciaphylococcus aupital personnel

mpling of the h1.66% were MR

ords personnel, Hospi

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ccus aureus inKolhapur

sh

ollege, Kolhapur

dozen species ocococcus aureus,

of penicillin inramatically. Howenicillin resistantd in response. MFirst Methicillinnce then it becaRSA. Methicillinocci spp. has tance to antibiotntracted in the atients, hospital

n in the hospitaical samples re

pur within a percus isolates. Stapand studied for Misolates from t

ureus i.e. MRSA13.33% were Mhospital environRSA and 6.94%

ital environment

Conference on

1st Century February 2011 tract Book

Page 54 

n D. Y.

r.

ccurring as is the most

n 1940 the wever, the t S. aureus

Methicillin , n resistant ame major n was later

a unique tics rapidly

hospitals. l personnel al. Regular eceived in riod of 12

aphylococci Methicillin he clinical

A and 44 ( MRSA and nment 204 were MR-

t.

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Progressiv

Modeand CDepa

 

ProductiInd

1. Lecturer in

2.Hea

Xanththickening ageis its ability toquantity of guXanthomonaswas able to prcontaining banother ingrediepolymer mat milled to give

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

on of Xanthadustrial and K

Repe A. B., 1

n Postgraduate D

ad, Department o

han gum is polyent and stabilizeo produce a largum. Xanthan gucompestris is th

roduce extracellunana peel extracents. After fermbe precipitated a powder that w

Arts, Science 11005

biology

PP4an Gum By XKitchen Was

1Dhutekar S.

Department of M

Latuof Microbiology

ysaccharide used er. One of the mge increase in vium helps to crehe pathogen isoular polysaccharict as sugar sourcmentation that c

from the mediuwill be readily so

Mi

Published on 25t

48 Xanthomonasste as a Produ

S., 2Dr. Ranje

Microbiology, Daur Dayanand Scien

as food additivemost remarkable iscosity of a liqueate pleasant texolated from the iides when inocuce, cabbage extrcan vary in timum by the addi

oluble in water.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Compestris uction Media

ekar M. K.

ayanand Science

nce College, Lat

e, commonaly usproperties of xa

uid by adding a xture in many iinfected leaves o

ulated in fermentract as nitrogen

me one to four tion of alcohol,

Conference on

1st Century February 2011 tract Book

Page 55 

Using a

e College,

tur

sed as food anthan gum very small ce creams. of cabbage tation broth source and days. The

, dried and

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Progressiv

Modeand CDepa

 

SynthesiApplicatio

Rutuja

Departme

The ato 100 nanomof nanoparticZnO nanopacharacterizatioray diffractionwell diffusiontransmission textiles. Appnanoparticles future aspect o

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

is and Characon on Textiles

a R. Phatate,

ent of Biotechnol

application of nameters is an eme

les is an area of articles were synon was done usinn. The antimicron technique. Aand personal p

plication of efto textiles for

of this project.

ZnO Nanopar

Arts, Science 11005

biology

OP1cterization ofs as Antimicr

Rebecca S. T

logy, Modern CoShivajinagar, P

anoscale materiarging area of naconstant interestnthesized usingng UV spectroscobial activity of An awareness protection has lffective antibacproducing antim

Key Worticles, Antimicr

Mi

Published on 25t

12 f ZnO Nanoprobial Agents

Thombre and

ollege of Arts, ScPune 411 005

als and structureanoscience and nt for its wide rang simple chemcopy, Scanning eZnO nanoparticof personal sa

led to the deveterial agent an

microbial and U

ords robial agent, UV

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

particles and s and UV Abs

Sonal P. Bath

cience and Comm

es, usually ranginanotechnology

nge of applicatiomical method electron micrococles was studied anitation, contaelopment of annd UV absorb

UV absorbing te

V absorbers

Conference on

1st Century February 2011 tract Book

Page 56 

Their sorbers.

hija

merce,

ing from 1 . Synthesis ns. and their

opy, and X-using agar

act disease ntimicrobial bers- ZnO extiles is a

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Progressiv

Modeand CDepa

 

Candida G

Lecturer in De

Candeukaryotic, bublastoconidia.pseudohyphaeof normal florHowever, folspectrum anticaused after cgroups over pathogen, seveknown to ctransplantationconcern for CFluconazole adifficult to trefrom a deep -Physicians woof these drugAmphoterecininfection. Heruncontrolled Dand histopathoGram stain anbudding yeastagar. The absappearance ofrapid and cosCandida specinfections.

Candi

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Glabrata Infec

epartment of Mi

dida glabrata, fudding yeast, usu It is the onl

e. Candida glabrra of healthy indlowing the widfungal therapy, colonization by the past few yeral other speciecause opportunn, meningitis, pCandida glabratand can developeat. For this rea- seated source (ould tend to use gs. Patients witn-B, since this re is presentedDiabetes mellituologically as “sund Lactophenol t cells. Yeast colence of pseudoh

f dark pink to vist- effective diacies should be

ida glabrata, CH

Arts, Science 11005

biology

OP0ction of the N

Repo

Roma A. C

neetiroma@gcrobiology., Dr

Institute, Kformerly called ually round to ovly species of rata was considdividuals and rardespread use of

the frequency oCandida glabrat

years. While Caes like Candida tnistic infectionspneumonia and ta is that it is p resistance to son, it is clinica(i.e fluid or tissuan Azole agent

th Candida glais the only eff

d the case repous, having an inuspected mucormcotton Blue moulonies were seenhyphae and chlaiolet colonies on

agnosis of Candperformed on a

Key WoHROMagar Cand

Mi

Published on 25t

01 Nasal Septumort

Chougale

gmail.com .D. Y. Patil Med

Kolhapur Torulopsis glab

val in shape. It mgenus Candidaered as relativelrely causing ser

f Immunosuppreof mucocutaneouta has significanandida albicans tropicalis, C.kruss in septicemihaematologic mless susceptibleother Azoles. H

ally important thue) be fully specfor treatment dubrata should b

ffective therapy ort of a 58 yeanfected nasal sepmycoses”. Micrount of colonies

n on Blood agar amydospores onn CHROMagar Cdida glabrata. Sall clinical spec

ords dida Medium, Da

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

m – An Unusu

dical College and

brata is small umultiplies by proa that does noly non-pathogenrious infections iessive therapy wus and systemicntly increased in

remains the msei and C.glabraia, urinary tra

malignancies. The to AmphotereHence these infehat a pure growciated. Without ue to the decreasbe immediately

for Azole-resisar old male paptum, diagnosed

oscopy of infecterevealed small 3and Sabouraud’

n Dalmau plate cCandida medium

Screening for nocimen suspectin

almau Plate cultu

Conference on

1st Century February 2011 tract Book

Page 57 

ual Case

d Research

unicellular, oduction of ot produce nic and part in humans. with broad c infections n older age major yeast ata are now act, organ he primary ecin B and ections are th of yeast speciation,

sed toxicity placed on

stant yeast atient with d clinically ed tissue by 3-5 µ, oval ’s Dextrose culture and m offered a on-albicans ng mycotic

ure

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Modeand CDepa

 

Biological

D

The ivarious areas effect on varionanoparticles be toxic, flamattempt was mgreen synthesAzadirachta inand their antibEscherichia caureus and SPrince Aly Kcharacterized impregnated ostudied. The eantibacterial psheet coated wbactericidal tomaximum ansynergistic babiologically nosocomial in

Silver napneumonia, P

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Synthesis of

*Sab

Department of M

integration of naof medical scien

ous microorgani(SNPs), when sy

mmable and not amade to formulasis of SNPs, wndica and Caricabacterial activitycoli, Klebsiella

Steptococcus pyoKhan Hospital. S

by uv-vis spectronto cotton clotheffect of consecproperty was alswith SNPs could o the isolates an

ntibacterial activactericidal effect synthesized SN

nfections.

anoparticles, AntPseudomonas ae

Allium cep

Arts, Science 11005

biology

PP4Silver NanopAntibacteria

ah Shaikh an

Microbiology, the

anomaterials witnce. Silver has bsms present in mynthesized by wat all environmete a cost effectiv

which were syna papaya with Ay was studied us pneumonia, Pogenes obtainedSNPs (1:2) showroscopy and scanh and their antibutive washing oo investigated. Tbe retained up t

nd of all differevity. These plan

with AgNO3. TNPs incorporate

Key Wotibacterial, Planteruginosa, Staphpa, Azadirachta

Mi

Published on 25t

49 particles andal Activity

nd Shilpa Sabn

e Institute of Scie

th biology is finbeen long recog

medical and induwet chemical reduent friendly. Theve and environm

nthesized using AgNO3 solutions sing disk diffusioPseudomonas ad from bed sheewing maximum nning electron mbacterial activityof the coated disThe antibacterialto 3 washings. Sient plant extractnt extracts wer

This work demoned in cotton b

ords: t extracts, Escherhlococcus aureusindica, Carica p

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

d Evaluation o

nis

ence, Mumbai.

nding wide applgnized as havingustrial processesuction method arerefore in presenment friendly tec

extracts of Allin varying ratio

on method on isaeruginosa, Stapets of casualty wantibacterial act

microscopy (SEMy on the same issks with distilledl activity of the ilver nitrate on itts, Carica papayre further foundnstrates the possbed sheets to

richia coli, Klebs, Steptococcus ppapaya.

Conference on

1st Century February 2011 tract Book

Page 58 

of Their

icability in g inhibitory . But silver re found to

nt study, an chnique for lium cepa, s (1:2, 1:4)

solates viz., phlococcus ward from tivity were

M) and then solates was d water on cotton bed ts own was ya showed d to show sible use of

minimize

bsiella pyogenes,

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Progressiv

Modeand CDepa

 

Study of O

Dep

Contalbumin preseby many ocuvarious MPS across MumbVarious isolatProteus vulgasamples. Variantimicrobial diffusion andstudied.Antiadcoverslip adhadherence of asolutions ReNWhere Flexileaffectivity of tcleaning devic

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

cular Microfof MPS

*Sag

partment Of Mic

act lenses have pent in tears are foular organisms. T

against these isbai and 50 samtes viz. Staphyloaris, Klebsiella ious multipurposactivity was studd Agar well dhesive activity

herence method all isolates wereNu and Compleens and ReNu sthese MPS was ce.

Arts, Science 11005

biology

PP5flora, AntimiS Solutions on

gar Vandra &

sagarvandra@crobiology, Instit

proved to be useound to provideTherefore attemolates. A survey

mples were colleococcus aureus. pneumoniae, P

se solution useddied against thesdiffusion methof these isolateand by scanninfound to be dec

ete were found showed maximafound to increas

Mi

Published on 25t

50 crobial and An Ocular Isol

& Shilpa Sabn

@gmail.com tute Of Science, M

eful for visual coa good environm

mpt was made toy was carried ouected from peopStreptococcus m

Pseudomonas sppd for lens cleaninse isolates using hod. Their pee was studied bng electron miccrease in presencto show maxim

al antiadhesive pse when used in

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Antiadhesivelates

is

Mumbai 400032

orrection but lysment for biofilmo study the micut at 17 differenple between 12mutans, Candidap. were detecteng were assessemodified ISO 1

ercentage inhibby contact lens croscopy. The sce of MPS. Out mum antibacteriaproperty. The anpresence of vib

Conference on

1st Century February 2011 tract Book

Page 59 

Activity

2

ozyme and m formation croflora on nt opticians 2-49 years. a albicans,

ed in these ed for their 4729, Disc

bition was adherence,

strength of of all MPS al activity. ntiadhesive

bration type

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Progressiv

Modeand CDepa

 

Identificat

1Dr

Tobaand healthy toPolyacrylamid(Enzyme Linkthe presence otransferred oninterest. LaterGel Electrophand thus creat

ToMV, SDS

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

tion and Extr

Samridd

r. D. Y. Patil Inst

acco mosaic viruobacco leaves ade Gel Electrophked Immunosorbof viral protein. nto Nitrocellulosr the genomic D

horesis. Certain ve a new hybrid p

S-PAGE, ELISA

Arts, Science 11005

biology

PP5raction of TobMolecular T

dhi R. Sharma

titute of Biotechn2INDAM, Ba

us is found in toare analysed by horesis). The totbant Assay), whe

The protein is se Membrane. T

DNA is extractedviruses such as Tplant with health

Key WoA, Genomic DNA

Mi

Published on 25t

51 bacco Mosaic

Techniques

a1 and Aparna

nology and Bioinangalore

obacco plants. ISDS-PAGE (S

tal viral protein ere the color chaelectrophoresed This gives the pd (Genomic DN

ToMV are very ehy factors.

ords A, Nitocellulose

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

c Virus (DNA

a Das2

nformatics, Pune

In this project thSodium Dodecyl

is separated usiange in ELISA p

and the proteinposition of prot

NA Extraction) beasily isolated an

Membrane, Aga

Conference on

1st Century February 2011 tract Book

Page 60 

A) Using

e

he infected l Sulphate-ing ELISA

plate shows n bands are tein of our by Agarose nd removed

arose Gel

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Progressiv

Modeand CDepa

 

Phyloge

1Dr

The (insulin) in siNCBI (NationMus musculCaenorhabditalignment waprotein sequenpfam, embossspecies relatiostudies of diff

Phylogenetics

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

enetic Analysi

Sand

r. D. Y. Patil Inst

project focusesix different specnal Centre of Biolus, Gallus gatis are sequencas done and phnce was constucs, cath, target p,on on the basis ferent species.

s - to show evolu- cattle, Gall

Arts, Science 11005

biology

PP5is of Insulin (

Too

eep Gajbhiye

titute of Biotechn2Hydera

s on the phylocies the query sotechnology Infoallus, Drosophced using the hylogenetic analcted. the tools u hyperchem, heof insulin struc

Key Woutionary relationslus gallus - roste

Mi

Published on 25t

52 (Protein) Usils

e1, Mr. Sunaka

nology and Bioinabad

ogenetic and stequence of thes

ormation). The sphila melanogas

tools of bioinlysis was perfo

used for the analx, signal p. are cture. It can be

ords ship, Mus muscur, Caenorhabdit

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ing Bioinform

ar2

nformatics, Pune

tructural study se species was tpecies such as Bster, Rattus nnformatics. Theormed dendrogrlysis are blast, wused and is comused for the ta

ulus - house rat Btis - worm

Conference on

1st Century February 2011 tract Book

Page 61 

matics

e

of protein taken from

Bos Taurus, norvegious, sequence

ram of the workbench, mpared the axonomical

Bos Taurus

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Modeand CDepa

 

Microbi

Sayali Ma

Microbial Nitro

highly energeEnvironmentaenvironment developed in(FOX-7). It iinsensitive exacetamidiniumwhich on nitrwith two nitWastewater gFOX-7 in highlike methaemenvironmentalobtained by sproduction sitisolate of Micwhen incorporconcentration from 20% to 4were also ablthus can be us

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

al Degradatio

ahajani, Abhij

l Sciences Divisi

oexplosives cometic materials. Ually transformedand human bei

nsensitive high is one of the imxplosive (IHE). m chloride with ration followed tro and two amgenerated duringh amounts and itmoglobnaemia. lly transformedsoil enrichment e. Out of these f

crococcus sp. corated in synthetiof FOX-7 as 40

40% after supplee to grow in th

sed for bioremed

Arts, Science 11005

biology

MPPon of Diamin

Nitroexp

it More, Seem

ion, MACS-Agha

mprise a major Use of nitroexpld products of ings. Present stu

energy nitroexmportant nitro eIt is synthesizediethylmalonateby hydrolysis gmino groups a

g the productiont appears to be aTherefore bio

d metabolites istechnique using

four bacterial isoould use FOX-7 ic medium at th0 mg/l in the synementation of me wastewater ge

diation purpose.

Mi

Published on 25t

P53 nodinitroetheplosive

ma Sarnaik an

arkar Research I

component of losives is impor

these nitroexpudies include bxplosives 1,1-dexplosive of clad by HEMRL Pe to obtain 2-megives FOX-7. It attached to carbn of FOX-7 is hallergic to humandegradation stus necessary. 13g the soil samplolates comprising

as the sole sourhe concentration nthetic medium,

medium with 0.01enerated during

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ene, a High E

nd Pradnya K

Institute, Pune 4

nitro compoundrtant for nation’plosives are h

biodegradation odiamino-2,2-dinitass called as hiPune, by the treethyl-pyrimidineis an aliphatic

bon-carbon douhighly acidic ann beings causingudy of FOX-73 bacterial isolles collected frog 3 Actinomycetrce of carbon anof 500 mg/l. Atthe removal wa

1% peptone. Theproduction of F

Conference on

1st Century February 2011 tract Book

Page 62 

Energy

Kanekar

11 004

ds and are ’s security. harmful to of recently troethylene igh energy eatment of e-4,6-dione compound

uble bond. nd contains g symptoms 7 and its lates were om FOX-7 tes and one nd nitrogen t the initial s enhanced ese isolates FOX-7 and

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Microbial

Snehal Bar

Microbial

Chemhowever, theydue to large vchemicals eithEffluent treatindustry. Sincsmall scale unfor cluster of in nature. It imicrobial cultchemical induMagenta, CryTurbuphos, Hhydrochloridewastewater is matter. It conextent of 800was developedbacterial consfrom an initialscale in the lafrom initial 68pilot plant of sSphingomonasFlavobacteriuBacteroides, Rin reducing thpurpose.

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

BioaugmentCommon E

ri, Nilesh Sonu

Sciences Divisio

mical industries y also contributevolume of efflueher in the form tment plant thuce it is difficult nit, existence of such small scales quite difficult ture. The wastewustries producinstal violet, Acid Hexaconazole) e, Cyclosporin),

highly acidic inntains organic lo0 mg/L and amd using the bactortium used at sl 8000 mg/l to 1

aboratory. The ex800 mg/l to 396size 25 m3. The s, Comamonas

um, Aeromonas,Ralstonia and Ache organic load

Arts, Science 11005

biology

OP1ation for Tre

Effluent Trea

une, Amit SinP. P. Kan

on, MACS - Agh

play an importe to environmenent generated duof unused raw

us becomes an to set up an effcommon effluen

e units. The wasto treat such ty

water under studng dyes and pyellow, Fast gar, pharmaceutica, petrochemicaln nature and darkoad in terms of

mmoniacal nitrogteria isolated frosite. The bacteria1500 mg/l at flasxperiments carri7 mg/l at 25 l scbacteria belonges, Cupriavidus, Bacteroides, chromobacter, a

d and thus indic

Mi

Published on 25t

10 eatment of Inatment Plant

nnarkar, S. S.nekar

harkar Research

tant role in devntal pollution p

uring the producmaterial or unreunavoidable c

fluent treatmentnt treatment planstewater in CETPype of mixed effdy is from a CETpigments (e.g. Drnet GBC); pestals (Bitamethazls (spray oil, lk in appearance Chemical Oxyg

gen up to 900 mom the soil in thal consortium desk level, and 550ied out at site recale and from 30ed to genera Ochs, Xanthomona

Ralstonia andall other genera cated their pote

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ndustrial Effl(CETP)

Sarnaik, G. K

Institute, Pune 4

velopment of anarticularly wate

ction and presenecovered finishe

component of aplant independ

nt (CETP) becoP is usually hete

ffluent using a sTP receiving efflDirect blue, Yticides (Temephzone valerate, liquid paraffin)with appreciable

gen Demand (COmg/L. Bacterial che premises of Ceveloped lowere00 mg/l to 1820 esulted in remov000 mg/l to 140hrobactrum, Pseas, Klebsiella, d Achromobacte

were found to bential for bioaug

Conference on

1st Century February 2011 tract Book

Page 63 

uents in

K. Wagh,

411004

ny country r pollution ce of toxic ed product. a chemical dently for a mes useful erogeneous ingle, pure luents from

Yellow FG, hos, Ethion,

Fluoxetine ) etc. The e settelable OD) to the consortium CETP, and d the COD mg/l at 5 l

val of COD 0 mg/l at a

eudomonas, Bacillus,

er. Except be efficient gmentation

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DetermSa

Sh

1Department

Listertype of sanitizmonolate ). Lwhich helps bGG3V & BHhypochloride tween80 and Ethidium bromfor Mdrl geGentamycin, R

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ination of Reanitizers and

hahina Shaikh

t of microbiology

ria spp are ubiqzers and antibioListeria spp. couacteria to increaH2V were scre, dettole and amremarkable shi

mide agar plate ene. Both the Roxythomycin, A

Tween80

Arts, Science 11005

biology

PP5esistance of Ld Antibiotics

h, Abhay Raor

y New Arts Com

quitous in nature tics in the preseuld secreat extr

ase the unsaturatieened for deter

mpicillin. MIC weft was observedassay. Further cisolates showi

Azithromycin.

Key wo, MIC, Efflux pu

Mi

Published on 25t

54 Listeria monocin Presence o

rane and Dee

mmerce and Scien

& express the rence of tween 8racellular enzymion to saturationrmination of reere determine bed. Efflux pumpchecking of effluing susceptibilit

ords ump, Mdrl gene,

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

cytogens Towof Tween 80

epali Giremar

nce College, Ahm

esistance toward0 (polyoxyethel

me i.e glycosyl n ratio at 370c. Twesistance towardefore and after tr activity was cux was confirmety towards Am

, AST.

Conference on

1st Century February 2011 tract Book

Page 64 

wards

mednagar

ds different lin sorbitan transferase wo isolates ds sodium reatment of checked by ed by PCR mpicillin ,

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Antibac

Shi

Department

Growantimicrobialscoriander, nucumin, turmeron black pep(Curcuma lonand phytochepepper (seeds)and DMSO. Sbacterial patho

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

cterial ActivitBorne

ikha Malla, Sh

t of Microbiology

wing concern abs. Spices are theutmeg, cinnamonric, ginger exhibpper (Piper nignga.). Crude extrmical screening) and turmeric (

Spice extracts inogens.

Antibacteria

Arts, Science 11005

biology

PP5ty of Crude E

e Pathogenic M

hilpa Mujumd

y Modern colleg

bout safety of fe most commonn, lemongrass,bit antimicrobialgrum L.) cuminracts were used

g. Solvent extra(rhizomes); solven methanol & eth

Key woal activity, Piper

Mi

Published on 25t

55 Extracts of SpMicroorgani

dar and Madh

ge of Arts, Scienc

foods led to thnly used antimic

cardamom, blal activity. Presenn (Cuminum cy

for determinatioction was doneents used were mhanol showed an

ords nigrum, Curcum

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

pices against sms

huri Amrutkar

ce & Commerce,

he development crobials. Spices ack pepper whint investigation yminum L), andon of antibacter for cumin (seemethanol, ethanntibacterial activ

ma longa.

Conference on

1st Century February 2011 tract Book

Page 65 

Food

r

Pune 05

of natural like clove, ite pepper, is focused

d turmeric rial activity eds), black ol, acetone

vity against

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Evalua

Dep

A hatheir microbiacomes in varithe distinctivelaboratory. Suuseful in foodartificial synthHence the prenatural pigmewere isolated maintained bytests: macroscLeifsons, Casetheir pigment nutrient brothbetter pigmenisolate no-26 biomass produ200rpm at thewas at 120th hpigment produpresence of giron.The pigmactivity of pigcosmetic prodagainst E.coliobserved agaiusing human Rto check the ntest. Since pigbe used to disin beverages, c

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ation of AntibP

Sh

partment Of Mic

allmark feature oal colonies whenety of hues and e colour of theuch pigmentatiod, cloth, paintinghetic pigments esent work mainents from natural

from soil, watey refrigeration atcopic and microein hydrolysis, Iproduction abil

h media by specnt in King’s A m

and isolate nouction of isolate

e interval of 24 hhour followed buction determine

glucose as a C-sment was extractgment extract waducts by well dii, K.pneumonaeinst S.pyogen anRBC’s to check

non-toxicity of thgment showed a scover new drugcakes or to deco

Arts, Science 11005

biology

PP5bacterial ActiPseudomonas

ilpi.R.Chand

shilpichand@crobiology, Instit

of several pathogn propagated in

has A hallmarkeir microbial co

on comes in varg, cosmetics, pha

which create senly focuses on tl resources. Theer and contamint 40c. The isolateoscopic morphondole and H2S plity in King’s Actrometric meth

medium compareo-15). The direce no-26 in Kinghours was studieby decrease afteed for isolate noource, ammonia

ted by Blackwooas tested against iffusion techniqu, P.vulgaris, S.fnd P.merabilis. Tthe suitability of

he pigment whicgood antibacteris with broad spe

orate and display

Mi

Published on 25t

56 ivity of Pigmeaeruginosa

& R.B.Vaidya

@yahoo.in   tute Of Science,

genic microbes the clinical labo

k feature of seveolonies when priety of hues anarmaceuticals anerious environmthe production ae pigment produnated egg sampes were identifieology motility, production. The iA, Demoss and od at 520nm. O

ed to Demoss &Fct co -relationshg’s A broth at 3d and the maximr 144 hours. Th

o-26 were pH-7.a as a N- sourceod and Neish methe common patue. The antibacflexnerri, S.aureThe hemolytic af pigment for hu

ch was confirmedial activity and nectrum or it can the food items.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ents Produce

a

Mumbai 400032

is the distinctiveoratory. Such pigeral pathogenic mpropagated in thnd has often prond plastics as anment and safety and evaluation o

ucing Pseudomonles on cetrimide

ed by performingCatalase, Oxidaisolates were comFranks, Minima

Only, two isolatFranks broth mehip between pig

350C on rotary smum pigment anhe optimum con0 and temperatue and Fecl3 as aethod and the anthogens of food,terial activity w

eus; while no acactivity test was uman consumptiod by negative reno blood hemolybe used as a foo

Conference on

1st Century February 2011 tract Book

Page 66 

ed by

2.

e colour of gmentation microbes is he clinical oven to be

n option for problems.

of safe and nas species e agar and g following ase, Hugh-mpared for al Salt and tes showed edium (i.e.: gment and

shaker with nd biomass nditions for ure-35oC in a source of ntibacterial , water and

was noticed ctivity was performed

on and also sults of the ysis, it may od colorant

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Modeand CDepa

 

Biodegra

S

Hexais produced aleather tannersources and exceeding theother organs. genome. It microorganismreduce it to tPseudomonas,identify microwater. The samost of the inmicrobes had and analysed ffound that onlthen subjecteMingioli Synteffect of pH checked to opsewage watersto a concentrappm in 2 and was found ouwas 37 ºC andremediating csuch high conorganism is m

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

adation of He

Singh Viveka ,

avalent Chromius a by-product ories etc. Due tocontaminate th EPA standards It can also lea

affects plant ams, a large numthe non toxic T, Arthrobacter

oorganisms that aamples taken wendustrial effluent

the highest degfurther. The conly one isolate waed to increasingthetic medium anand Temperatuptimize the pars. Results showeation of 50 ppm5 days respectiv

ut to be 6 to 8. Td degradation wacontaminated sitncentrations and mesophilic.

Arts, Science 11005

biology

PP5exavalent Ch

, Srivastava S

Fergusson Co

um is a toxic, muof many industr

o its high solubihem. Consumpti

can lead to sevead to the develalso. Although mber of them caTrivalent Chrom

etc. The aim oare able to degraere sewage watets are discharge iradation potenti

nsortium was plaas responsible fog concentrationsnd the reduction

ure on the degrarameters for raped that the isolat

m with complete vely. The optimuThe optimum teas faster at 55 ºCtes due to its abalso its removal

Mi

Published on 25t

57 romium Usin

Shreya and Go

llege, Pune

utagenic and carcries like metal pility, it can reacion of Hexavaere damage to thopment of canc

Hexavalent Can tolerate high

mium. These incof our research ade high levels oer so as to obtain sewage. The al for Hexavalen

ated to obtain theor the degradatios of Hexavalenn was checked eadation potentiapid remediation te could degrade

and rapid degraum pH range of mperature for th

C. This isolate cobility to reduce from sewage w

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ng Microorga

ore Suneeti

cinogenic compolating, dye manch the undergro

alent Chromiumhe kidneys, lungcers and mutati

Chromium is hh concentrationslude genera likis to isolate, anf Hexavalent Ch

ain an indigenousample whose cnt Chromium we individual isolaon process. This nt Chromium ineach day up to dal of the isolate

of contaminatee Hexavalent Chradation of 10 ppthe isolate for d

he growth of theould be extremel

Hexavalent Chater treatment pl

Conference on

1st Century February 2011 tract Book

Page 67 

anisms

ound which nufacturing, ound water

m at limits gs, skin and ons in the

harmful to s and even e Bacillus, nalyse and hromium in us strain as consortia of as selected ates. It was isolate was n Davis –

day 10. The e was also ed sites or romium up pm and 20

degradation e organism ly useful in

hromium at lants as the

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Soura

D

Silvereduction, andobserved usinanoparticles 420nm form (EDX), ScannThe nanopartbacteria and gwas studied on

Silver nano

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Study of S

bh Mehta*, R

Dept. of Biotechno

er nanoparticlesd chemical reduing UV-Visiblewas studed by the UV-Vis sp

ning Electron mticles of Silver dgram positive ban cancerous cell

oparticles, UV-V

Arts, Science 11005

biology

PP5Synthesis of S

Rebecca Thom

ology, Modern C

s were prepareuction method. Te absorption s

the typical surfpectrum. We haicroscopy (SEMdemonstrated anacteria . The antiline The Human

Key Wo

Vis spectrum, SE

Mi

Published on 25t

58 Silver Nanop

mbre and Shu

College, Shivajin

d by bacterial The formation ospectroscopy. Tface Plasmon abave used energy

M) to characteriznti bacterial activi cancerous activn Monocytic cell

ords EM, EDX, Antib

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

articles

ubhangi Puran

nagar, Pune 05

reduction, Bioof Silver nanopaThe formation bsorption maximy dispersive sp

ze the Silver nanvity against gramvity of Silver nal line (THP1).

acterial, Antican

Conference on

1st Century February 2011 tract Book

Page 68 

nik.

o physical articles was

of Silver ma at 418-pectroscopy noparticles. m negative

anoparticles

ncerous.

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Decoloriza

Dep

Disch

aquatic ecosyeffluent bio tdegradation odecolorizing bBurkholderia aeration, % icarbon and nconditions reqyellow, whichculture was oDecolorizationshowed high disappearancedecolorizationdecolorizationwell-documenlemon yellow extract. The idyes. Adsorpstudied. Cells(30%) as comon alginate bcomparable. Poriginal dye. PTriticum aestiheight of shoo

Direct l

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ation of Texti

*Sunil. S.

partment of Micr

harge of textile stems and humatreatment proce

of dyes to non tbacterium was i

cepacia by 16inoculum, initianitrogen sourcequired for maxih was used as a observed at pH n was confirmed

peak at the e of peak and an is due to dn took place strinted reports. Bur

in presence of solate had an ab

ption of Direct ls immobilised in

mpared to free celbeads (11%) thPhytotoxicity andPhytotoxic effecivum was studiedot and length of l

lemon yellow, D

Arts, Science 11005

biology

PP5

ile Dye DirecCepac

Tiwari and Sa

robiology, Instit

wastewater contan health. This esses to producoxic metabolitesisolated from dy6S rRNA sequal concentration s was studied imum decolourimodel dye. The7.4 and incuba

d by UV-VIS spwavelength of

appearance of pdye degradationictly under aerobrkholderia cepa1% (w/v) glucosbility to decolorlemon yellow onn 7.5% Na-algills (77%) and cohe efficiency ofd microbial toxicct of Direct lemod by calculating leaf.

KeywoDecolorization, B

Mi

Published on 25t

59

ct Lemon Yelcia

angeeta. K. J

tute of Science, M

taining toxic dyestudy is part of

ce reusable wats by bacteria. Inye contaminated

uencing. The efof dye, initial with an aim t

ization and dege optimum dye-dation temperaturpectrophotometef 410nm. The peak at 298nm, n by Burkholdbic conditions, w

acia showed 99%se and 98% in prize five other stn Na-alginate benate matrix sho

onsidering the dyf the two systecity studies wereon yellow on grofactors, such as

ords: Burkholderia cep

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

llow by Burkh

angam

Mumbai 400 032

es can adverselyf efforts to deveter by decolorizn the present stud sample and idffect of pH, te

concentration to determine th

gradation of Dirdecolorizing actire of 30o C after. The initial dydecolorized dywhich indicate

deria cepacia. which is contrar

% decolorizationpresence of 1% (tructurally differeads and degradowed lower decye adsorption phems was not foe also carried ouowth of Phasealpercentage of ge

pacia, Phytotoxic

Conference on

1st Century February 2011 tract Book

Page 69 

holderia

2

y affect the elop textile zation and udy, a dye

dentified as emperature, of various

he optimal rect lemon ivity of the ter 7 days. ye solution ye showed ed that the

Optimum ry to other n of Direct (w/v) yeast rent textile

dation were colorization henomenon ound to be ut using the lus mungo, ermination,

city.

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PhytocheLeaves of B

*S.B.Dah

1 Departmen

2 Departm

Phycarbohydrateschloroform exracemosa Linmethod againStaphylococcuaeruginosa, Sand Proteus vagainst enteriacetate extracAntibacterial attempt to deenteric infectio

Antib

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

emical and AnBauhinia Rac

hikar1, 2, S. A.

nt of PharmaceuEduc

ment of Microbio

ytochemical scs, alkaloids, flavxtract, ethyl acenn. were preparenst certain enus aureus, KlebsSalmonella typhvulgaris. The Mic bacterial pathct were slightlyactivity of variovelop a new phon.

bacterial activity

Arts, Science 11005

biology

PP6ntibacterial S

cemosa Lam. Bacterial P

Bhutada2, D.

sbdahikar10@tical Microbiolocation and Resealogy, Sant Gadg

reening of thevonoids, steroid

etate extract andd and antibacter

nteric bacterial siella pneumoni

himurium, SalmoMethanol extractshogens than they higher antibaous extract of leaharmaceutical dr

Key Woy, Bauhinia race

Mi

Published on 25t

60 Screening of (Caesalpiniaathogens

H.Tambekar2

@gmail.com  ogy, Sanjivani Coarch, Kopargaon

ge Baba Amravat

e plant leaves ds, and tannins.d methanol extrarial activity were

pathogens sucia, Enterobacteronella typhi, Sts had wide rang petroleum ethe

acterial activity aves of Bauhiniarug from natura

ords emosa, enteric ba

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Solvent Extraceae) against

2, and S. B. Ka

ollege of Pharman ti University, Am

reveals the pr. Petroleum ethacts of leaves oe studied by disch as Escheri

r aerogenes, Psetaphylococcus ege of antibacterer extract, wher

than chlorofora racemosa was

al origin for pre

acterial pathogen

Conference on

1st Century February 2011 tract Book

Page 70 

racts of t Enteric

asture1

aceutical

mravati

resence of her extract, f Bauhinia c diffusion ichia coli, eudomonas epidermidis ial activity re as ethyl rm extract. s carried in evention of

ns

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Modeand CDepa

 

Viral D

1 Department2 Departm

Banaplant has an hbanana contailack of viable is vulnerable tproduction. Bin India (Joshplant virus wiused to detectprimer specifiResearch, Batemperature, famplification successfully owhich did notused to screen

RT

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Diagnosis by U

S. O

t of Biotechnologment of Biotechno

ana is an importaherbaceous stem n large, hard seeseeds, propagat

to many diseasesanana Chlorosisi and Joshi, 197th a tripartite get the presence oic to coat proteinangalore. Diffeforward and reve

protocol. The obtained. The ret show any amp

n a number of co

- PCR, Banana

Arts, Science 11005

biology

PP6Using RT – P

Chlorosis

O. Gupta1 and

gy, Modern Collology, H.P.T. Ar

ant fruit crop of tand belongs to teds but the domtion is done veges amongst which

s disease was firs4). The causativ

enome. In the cuof the virus fromn gene were procerent parametererse primer conccDNA was am

esults were complification with rms prior to plan

KeChlorosis Disea

Mi

Published on 25t

61 PCR: A Case

Disease

B. A. Aglave2

lege of Arts, Scierts and R.Y.K. Sc

tropical and sub-the family Musa

mesticated varietietatively, by meh viral diseases ast reported in Au

ve organism, BSurrent investigatim the infected pcured from Indiars such as ancentration etc wemplified and th

mpared with thatthe primers. Th

ntation, thus avo

y Words se, Musaceae, R

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Study on Ba

2*

ence and Commecience College, N

-tropical countriaceae. The wild res are seedless. ans of corms. Bare the major conustralia (Magee,V is a positive son RT-PCR tech

plant samples. Tan Institute of Honnealing time, ere used to stand

he product of 7t of healthy planhe current protooiding heavy loss

RNA virus, CMV

Conference on

1st Century February 2011 tract Book

Page 71 

nana

erce, Pune Nashik

ies. Banana relatives of Due to the anana crop nstraints in 1940) and sense RNA hnique was

Two sets of orticultural

annealing dardize the 700bp was nt samples col can be ses.

V

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Modeand CDepa

 

S. M. KulkaS.R., S

Department O

Evenused as a rawtemperature anto extensive microbiology project, an atteBasidiomycetebiopulp from and 7 isolates bagasse and sbiopulp since content produclignin contenfermentation substrates. Thinoculated in After 30 daysActinomyceteproduced was making paper ecofriendly papowder. The strength and oPolysaccharid

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Biopulp

arni, Navale SShirke M. D., K

Of Microbiology

n in the world ofw material in thnd pressure. Thidestruction of techniques mayempt is made to es, Bacteria anlignocellulosic of Bacteria fromoybean industrythey are easily aces pitches in th

nt of the raw was performed

he individual typthese substrates incubation, lign

es showed decreathen used to mahelps in specific

aper; we have aecofriendly pap

odorless. Furtherde and Aspargina

Arts, Science 11005

biology

PP6p - An Eco-Fr

S. K., SulkshanKokani N. F.,

y, H. P. T Arts an

f paperless offiche conventionalis process requir

plants, there y be explored to

prepare the ecod Actinomycetematerial, we ham termite guts. Ay waste were theavailable and coe final product imaterials usedto get maximu

pes of Actinomto carry out sol

nin content was ase in lignin conake eco-friendly c texture and streadded binding aper made using r the isolates of Aase.

Mi

Published on 25t

62 riendly Appr

ne H. C., SharBhavsar S. P

nd R.Y.K Scienc

ce, we are forced pulping procesres many chemias been envirohelp in avoiding-friendly pulp. Bes has been repave screened 14 Agricultural byp

e two substratesst effective. The.e. paper. Thus i

d for preparatioum reduction o

mycetes, Bacterialid state fermentdetermined by ktent upto 0.1% apaper. The additength of the finagents’ viz. Pullupullulan & gela

Actinomycetes c

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

roach

rma L. P., Kha., and Joshi S

e College, Nash

d to use papers.ss by cooking uicals and high enonmental imbalg the deforestratBiodegradation oported. Hence tisolates of Acti

products such asused for the pro

e presence of ligit is necessary toon of paper. Sof lignin contena and also constation at room tekappa number. Tand 0.356%.The tion of binding aal product. Whileulan, gelatin & atin gave paper can be exploited

Conference on

1st Century February 2011 tract Book

Page 72 

andelwal S. A.

ik 422005

Plants are under high nergy. Due lance. The tion. In our of lignin by to produce inomycetes s sugarcane oduction of nin in high reduce the Solid state nt in these sortia were emperature. Two of the biopulp so

agent while e preparing Fenugreek with good to produce

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Plasmid PClov

Te

Modern C

Pseudnosocomial inPseudomonas factors and Rantibiotics duepumps and prResistance is (plasmid-encoclove oil agaidone. Garlic Gram-positiveantagonist effGarlic Extractby disc diffusextract and 8-isolates was dnot detected in

Pseudomo

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Profiling ande Oil on Clin

ejlaxmi Inam

College of Arts,

domonas aeruginfection. It exhi aeruginosa ma

Resistance Transe to low permearoduction of plaeither innate re

oded). Antibacteinst multidrug reis reported to ee and Gram-nefect against Gramt and Clove Oil wsion method. Inh-33 mm by clovedone by the midn the isolates tes

onas aeruginosa

Arts, Science 11005

biology

PP6

d Antibacterianical Isolates

dar, Milky Sa

Science and Com

inosa is an opporibits an innate raintains antibiotfer Factors. Thebility to outer m

asmid encoded eesistance (chromerial susceptibilitesistant clinical exhibit broad spegative bacteria.m-negative bactewere used to chehibition zones re oil were obserdi-prep and Kadted so far.

Key Wo

, Aqueous Garli

Mi

Published on 25t

63

al Activity ofof Pseudomo

ahukar and Ka

mmerce, Ganesh

rtunistic pathogeresistance to a tic resistance ple bacterium is n

membrane, constiextended-spectru

mosomally-encodty testing of aquisolates of Pseu

pectrum antibact. Clove oil is eria. Different ceck antibacterialranging from 8-rved. In additiono and Liu meth

ords c Extract, Clove

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

f Garlic Extraonas aerugino

avita Parekh

hkhind, Pune- 41

en, and a commowide range of lasmids, both R

naturally resistanitutive expressioum β-lactamasesded) or acquiredueous extract of udomonas aerugterial activity ag

also known toconcentrations ol activity on fifte-25 mm by aquen plasmid profilinhod. However pl

e oil, Plasmid pro

Conference on

1st Century February 2011 tract Book

Page 73 

act and osa

11053.

on cause of antibiotics. Resistance-nt to many on of efflux s (ESBLs).

d resistance f garlic and ginosa was gainst both o have an of Aqueous een isolates eous garlic ng of eight lasmid was

ofiling.

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Screenin

1D2Head

Lignispecifically wto fragment itlignin is carriperoxidase anforms family fungi for prodoxidize a varie

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ng of Fungal

Udata M. A.*

Department of Mid, Department of

in is an insolubwhite rot fungi det & diffuse to thed out by lignol

nd lignin peroxiof isoenzymes.

duction and assaety of phenols an

Lignolytic enz

Arts, Science 11005

biology

OP0Isolates for L

Peroxi

*, Joshi A. J.1

Microbiology, Dayf Microbiology, D

ble, high molecegrades it extrache hyphae & crolytic enzyme syidase. Mangane The aim of pr

ay of manganesend dyes.

Key Wozymes, Mangane

Mi

Published on 25t

04 Lignolytic Enides

1, and Ranjek

yanand Science Dayanand Scien

cular weight pocellularly. The fuoss the cell mem

ystem which incse peroxidase iresent work is se peroxidase usi

ords ese peroxidase, W

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

nzyme, Mang

kar M. K..2

College, Latur. nce College, Latu

olymer. So, varungi attack the lmbrane. Biodegrludes laccases, ms heme peroxidscreening of theing the property

Wood bark.

Conference on

1st Century February 2011 tract Book

Page 74 

ganese

ur.

rious fungi ignin so as radation of manganese

dase and it e lignolytic

that it can

 

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Screenings

D

Lipasmicroorganism3.1.1.1). The these lipases.important nosvirulence factAcinetobacterRhodamine Bisolates were esterase positperformed. OnB method theproduced by taccount of theB was sensitiinclude purifiproperties of A

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

g of Lipases aspecies Isolate

Urvi

Department of Mi

ses (triacylglycems either alone opresent study a The organism

socomial pathogtors it possessesr spp. for prod, Phenol red andshown positive

tive. For confirmn phenol red agae same 8 isolatethese isolates weese methods showve and efficient

fication, molecuAcinetobacter lip

Acinetobacter, L

Arts, Science 11005

biology

PP6as Potential Ved from Diffe

Doshi & Kari

karishma@unicrobiology, Uni

erol acylhydrolaor together with ims at understan

m chosen for thgen which has n. In the present

duction of lipasd Tributyrin Agar

for lipase prodmation, Phenol ar 8 isolates weres were screeneere further quantwed that Rhodamt in screening thlar characterizapases.

Key WoLipases, Virulen

Mi

Published on 25t

64 Virulence Facerent Hospita

ishma Pardes

nipune.ac.in iversity of Pune,

ases; EC 3.1.1.3)esterases (carbo

nding the possibhe study is Acinot been studiedstudy, we scree

ses by three dr Plate assay. By

duction some of red assay and

re shown to proded positive for ltified by titrimetrmine B plate asshe lipase producation & studyin

ords

nce, Rhodamine B

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ctor in Acinetals in India

si*

Pune 411 007

) are produced oxylic ester hydrble virulence prinetobacter, knod much for the ened 42 clinical different plate ay Tributyrin Agawhich also couRhodamine B

duce lipase. By Rlipase productioric method. A co

say at 0.01% of Rcers. Future studng the potential

B, Titrimetry

Conference on

1st Century February 2011 tract Book

Page 75 

tobacter

by various rolases; EC roperties of own as an

variety of isolates of

assays viz. ar assay, 15 uld be only

assay was Rhodamine on. Lipases omparative Rhodamine dies would l virulence

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In Vitro St

Vais

Natur

being used sidistinct from oinexpensive, Umedicines. Thliterature survprovide a scieresistant pathopathogens likeEscherichia cdiscover newmechanism opossessing anestimating theeither crude eethanol, and mwell diffusionPsoralea coryjatamansi, SmGymnema sylmethanol extractivity againwas observedformulating thinfections and

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

tudies of Ant

shali Prabhun

Abeda I

re has blessed Iince times immother fields of mUnani medicineshe prime reasonvey is either in Pentific base and ogens like MRSe Staphylococcusoli are responsib

w antimicrobial f action. Unanintimicrobial proe antimicrobial aextraction or Somethanol are emn assay. Screeninylifolia, Swertia milax glabra, Wrilvestre against Gracts of Psoralst Candida albid with Pseudohe extracts into

d prove to be a bo

Unani System

Arts, Science 11005

biology

PP6timicrobial Pr

Medicinal

ne, Namra Gh

Inamdar Senior C

India with immememorial to curemedicine as the ds provide an eff

n for these mediPersian, Urdu or documentation f

SA, skin infectios aureus, Candidble for severe skcompounds witi plants producioperties can be ctivity of these poxhlet apparatus

mployed for this png was carried o

chirata, Embelightia tinctoria,

Gram positive, Glea corylifolia acans and Staphyomonas aerugimedicines Una

oon for the socie

Key Wom, Drug resistan

Mi

Published on 25t

65 roperties of El Plants

ansar and Su

College, Camp, P

ense variety of me diseases. Unadrugs it uses are fective and safe icines not being Arabic language

for this system. ons are becominda albicans, Psekin infections. Tth diverse cheming wide range

harnessed to tplants, active pris. Different solvpurpose. The extut using methanlia ribes, RubiaPicrorhiza kurr

Gram negative aand of Embeliaylococcus aureunosa and Esc

ani system can pety.

ords nce, Antimicrobia

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Extracts from

upriya Bhopal

Pune

medicinal plantsani system of m

natural. Being nalternative to thwidely known

e. Hence there iDue to emergen

ng very commoneudomonas aerugThere is a growimical structures

of secondary mtreat skin pathoinciples are extravents such as ctracts are then su

nol extracts from a cordifolia, Naroa, Cyperus rotand fungal pathoa ribes showed us while minimucherichia coli. provide a cure t

al activity

Conference on

1st Century February 2011 tract Book

Page 76 

m Unani

le

s which are medicine is natural and he existing is that the s a need to

nce of drug n. The skin ginosa and ing need to and novel

metabolites ogens. For acted using chloroform, ubjected to

m plants like ardostachys tundus, and ogens. The maximum

um activity Thus, by

to the skin

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Lignin Deg

V. A

Depar Ligni

due to its inproperties, tencolored effluehazardous. Milignin and a wniche eight baout of which tof lignin. Crudof methylene mill effluent g50% to 58% peroxidases frpaper-pulp mibased effluentefficiently usindustries.

Lignin perox

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

grading PeroxTe

A. Tile *: Bor

rtment of Microb

in, the nature’s pntense unaesthends to be a recents due to presicrobial extracelwide range of c

acterial and two athree bacterial ande extracts of ligblue dye during

gave 60% to 75%decolourization

from mixed consill effluent and t which is signi

sed in biobleac

xidase, Biobleach

Arts, Science 11005

biology

PP6xidase in Bioxtile Dye-Bas

khataria B. V

vaishali_tile@y

biology, K.T.H.M

plastic is the majetic brown coloalcitrant composence of large allular lignin perocomplex aromatactinomycetes isnd one actinomygnin peroxidase enzyme assay.

% color reductionwas observed.

sortia gave 80%70% to 75% deificantly high. T

ching and biod

Key wohing, Paper-Pulp

Mi

Published on 25t

66 bleaching of sed Effluents

V, Bhalerao V.

yahoo.co.in M. College, Nash

ajor pollutant froor, hydrophobicound. Textile dyamount of mixtuoxidase enzyme ic dyestuffs. Fro

solates were screycetes were ableenzyme gave 60The studies on bn and in case ofThe heterogene

% to 85% color ecolourization inThis system of

degradation of

ords: p mill effluent, T

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Paper-Pulp s

B, Aher N. S.

ik, Pune Univers

om paper-pulp mcity and poor mye-based industrure of dyes whhas a potential

om various enveened for lignoly to decolorize 440% to 76% decobiobleaching of f textile dye- baseous combination

reduction in trn treatment of tlignin peroxidaeffluents from

Textile dye-based

Conference on

1st Century February 2011 tract Book

Page 77 

Mill and

.

sity

mill effluent mechanical ries release hich is also

to degrade vironmental ytic activity 4% to 49%

olourization paper-pulp

sed effluent n of lignin eatment of textile dye-ase may be

respective

d effluent

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Bacteria

V. A

Depar

The dis an importannanoparticles to obtain becacontrolled reasynthesis but great concern stabilized diresuch as bactemetals througinteresting as bacteria is invsilver resistantextracellular, t24 hours. AgNvessels that conanoparticles and UV-visibEscherichia crange of 12–2effect is dose Gram-positivepathogens of clinical isolatAspergillus fuanatomy and considerable applications.Inefficient remoand removal o

Silver-Nan

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

al Synthesis o

. Tile*., S. P.

tment of microbi

development of nt aspect of nanare available, buause it depends action environmthese methods ufor environmen

ectly in the proceria, yeast, and gh reduction of tnanofactories. In

vestigated by bot organisms werthe test strains wNO3 at concentrontained the supethus produced le spectroscopy

coli and Bacillu25 nm with incr

dependent and e organisms.

the skin weretes and ATCC umigatous The rspore germinatiantifungal ac

n the area of watoval of pollutantof chemical and b

notechnology, Ba

Arts, Science 11005

biology

PP6of Silver Nan

Pawar., W. R

vaishalitile@yiology, K. T. H. M

reliable processe

notechnology todut a narrow and on the adjusted

ment. Colloidal muse toxic chemicntal reasons. Theess by proteins.Afungi play an imthe metal ions, n this study, the th intracellularly

re grown in convwere cultivated iration of 10mMernatants of Eschwere characteri. The silver nan

us subtilis.The preased stability ais more pronouAntifungal eff

investigated. Nstrains of Tric

results showed non. Thus, the st

ctivity, deserviter purification, ns and germs. Thbiological substa

Key Woacteria-Nanopart

Antimicr

Mi

Published on 25t

67 oparticles an

R. Sayyed., an

yahoo.co.in   M. College, Nas

es for the syntheday. Many synthcontrolled size p

d concentration metal particles cacals in the synth nanoparticles syAlthough it is knmportant role inonly recently thproduction of siy and extracelluventional media cin their special c

M is separately aherichia coli, , Bized by transmisnoparticles wereprepared silver and enhanced an

unced against Grfects of silver Nano-Ag showechophyton rubrunano-Ag exertedtudy indicates thng further innanotechnology

he nanoparticles ances.

ords ticles, Extracellurobial

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

nd Its Applica

nd A.V. Bhosa

sik, Pune Univer

esis of silver nanhetic procedurespreparation seemof reacting chem

an be obtained bhesis protocol, wynthesized biolonown that micron the remediatiohis approach is lver nanoparticle

ularly. For intraccontaining AgNconventional conadded to the eacBacillus subtilis,.ssion electron m

e effectively pronanoparticles wnti-bacterial potram-negative bananoparticles

ed potent activium , Candida d activity on thhat the nano-Agnvestigation fo

offers the possibmay be used fo

ular, Intracellular

Conference on

1st Century February 2011 tract Book

Page 78 

ations

le

rsity

nomaterials s for silver ms difficult micals and y chemical

which raises ogically are oorganisms on of toxic considered es by some

cellular, the O3, and for

nditions for ch reaction . The silver microscopy oduced, by, were in the tency. This acteria than on certain ity against albicans ,

he mycelial g may have or clinical bility of an

or detection

r, TEM,

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Modeand CDepa

 

Study of Gr

Departmen

ESBLstudies have rproducing bacthe study wasCenter, Kadammultiple drug May 2009. A isolated from disc diffusionconfirmed by (42.13%) wersensitivity to Jarlier DDST were DDST psensitivity tesPiperacillin-ta(39.75%) & KMajority of thfollowed by su

Extended Sp

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

f Extended Spram Negative

V.S.Vat

nt of Microbiolog

L producing Grareported on the pcteria may have es carried out at Dmwadi, Kolhapuresistance in famtotal number ofvarious clinical

n test as per CLusing DDST (Dre potential ESthird generationfor these 83 iso

positive. All thst (AST). All thazobactum (87.5%Klebsiella pneumhe ESBL produurgical ward 16

pectrum beta-La

Arts, Science 11005

biology

OP0pectrum Betae Bacilli in Fa

tkar, P. G. Sh

vsatish999@red

gy, Dr. D. Y. Pa

am Negative Bacprevalence of Eevolved in severDept of Microbi

ur, to examine themily Enterobactf 197 GNB belosamples & stud

LSI (Clinical LabDouble Disc SynSBL producers n Cephalosporinolates. Seventy

he ESBL produchese 70 isolates%) and Amikaci

moniae 16 (37.20ucers were isola(22.85%) and fr

Key Woctamases (ESBL

Mi

Published on 25t

09 a-Lactamase amily Enterob

adija, S. J. Gh

diffmail.com   til Medical Colle

cilli is a worldwiESBL producers ral hospitals all oiology, D. Y. Pae incidence of Eteriaceae duringonging to familydied for ESBL prboratory Standaergy Test). Out on the basis os (3GC). ESBL (35.53%) isolatecers were testes were susceptibin (81.2%). Amo0%) were most ated from the mom ICU 12 (17.

ords L), 3GC, Resistan

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

(ESBL) Prodbacteriaceae.

hosh

ege, Kolhapur, 4

ide problem. Altin Indian hospit

over the countryatil Hospital and

ESBL producing g the period of Dy Enterobacteriaroduction by Ki

ards Institute) stof 197 isolates,

of resistance or production is d

es out of these d for MIC andble to Imipenemong these isolatecommon ESBL

medical ward 2014%).

nce, Enterobacte

Conference on

1st Century February 2011 tract Book

Page 79 

ducing

416006.

though few tals, ESBL . Therefore d Research strains and

Dec 2007 to aceae were irby- Bauer tandards & 83 isolates decreased

detected by 83 isolates

d antibiotic m (100%), es E.coli 33

producers. 0 (28.57%)

eriaceae.

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AccumuPseud

Vid

PHA

organisms. Buhydrocarbon SStyrene is releprocessing indrespiratory tracytoplasm of nutrient enviroPseudomonas and aliphatic thermoplastic disposable iteantibiotic. Ourcause environand can be usand also envproducts. It is derived from pof the industri

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

ulation of Polydomonas spp

dya N. UdawaNew Arts

A are polyester ofut PseudomonasStyrene, which iseased in milliondustries. Styrenact,and nervousmicroorganism

onment such as spp. Provides aPHA accumulatpolyester. It i

eams,food packar routinely used

nmental hazards.ed as alternativevironmentaly haimportant for thpetroleum oil suies and society.

Arts, Science 11005

biology

PP6yhydroxyalkand Effect of

Accumu

ant, Smita A. Comm. and Sci.

f various hydroxs can produce s also much resis

ns of kilograms ine is a suspectes system. PHA

m and it acts as nitrogen deficiena new link betwtion. PHA posses used in the aging, biodegradpetroleum based

. But the PHA ae to conventionaazardous styren

he global commuuch as plastic. PH

Mi

Published on 25t

68 kanoate (PHAf N2 Concent

ulation

Daule and D. College, Ahmed

xyalkanoate whicPHA efficientlystant to microbiain a year from pd carcinogen,teraccumulated asstorage source

ncy. The converween an aromatiess properties siproduction of pdable rubber,in d plastics are noare the bioplastil plastic for the

ne is convertedunity to have an HAs at least will

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

A) from Styreraction on PH

. D. Giramkardnagar

ch are synthesizey by using toxial degradation. petrochemical anratogen ,toxic ts discrete granu

of carbon in ursion of styrene ic environmentaimilar to variouplastic utensils cosmetic,medicn biodegradableics which is biobetterment of en

d into ecofrienalternative for thl be a solution fo

Conference on

1st Century February 2011 tract Book

Page 80 

ene by HA

r

ed by many c aromatic

nd polymer to kidneys, ules in the unbalanced to PHA by

al pollutant us synthetic

and other ine and as

e and hence degradable nvironment dly useful he products or the most

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Modeand CDepa

 

Biosurf

Vijendra

1D2Institute o

A main PG mediumand was partpreparative thpowder made lowered the smicelle concekerosene-wateagainst Staphyalso able to bioremediationkilling of HeLaeruginosa 0biosurfactant anumber of environmental

Biosurfac

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

factant Produ

A. Kavatalka

Department of Mof Bioinformatics

arine isolate of Pm at pH 7.2, 30oCtially purified

hin layer chromup of lipid and

surface tension entration of 25er interface. Partylococcus aureu

chelate chromn. The crude biLa cells. The pa1 and 74% Acalso had an intebeneficial traitl and biomedical

ctant, Pseudomo

Arts, Science 11005

biology

OP0uction by a M

r1, Smita S. BBalu A. Cho

directoribb@un

Microbiology, Uns and Biotechno

Pseudomonas aeC at 120 hours. Tby extraction w

matography. Thed polysaccharide(SFT) of distill5µg/L and redtially purified bious, Bacillus subtmate and ferrouiosurfactant at cartially purified bcinetobacter baunse antioxidant ts make this l applications.

Key Wonas aeruginosa,Antioxidant, Me

Mi

Published on 25t

07 Marine Strain

Bhuyan1, Peehopade1,2*

nipune.ac.in iversity of Pune,logy, University

eruginosa produThe biosurfactanwith chloroform biosurfactant ie with a yield oed water to 49.

duced interfaciaosurfactant exhibtilis, Bacillus puus ions indicat

concentration 50biosurfactant dis

umannii biofilmactivity of 83.55

biosurfactant

ords SFT, IFT, Antimetal chelation

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

n of Pseudom

hu S. Pardesh

, Pune 411 007 of Pune, Pune 4

uced a potent biont is a secondary m: methanol fois a brown, watof 1g/lit. The bio.377 mN/m, had

al tension (IFTbited antimicrobumilus and E. cting its potenti

00 µg/ml exhibitsrupted 97% Pses. Additionally, 5 % per 20 minuexcellent cand

microbial, Antic

Conference on

1st Century February 2011 tract Book

Page 81 

monas

hi1 and

411 007

osurfactant metabolite

ollowed by ter soluble osurfactant d a critical

T) between bial activity coli. It was ial use in ted 83.9 % eudomonas the crude utes. Large didate for

cancer,

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Modeand CDepa

 

Ut

Vynk

Departm

ActinBiosurfactant.biosurfactant containing moshown by Strnitrogen sourc(1g%) & production. Pu

B

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

tilization of M

katesh. M. SaShilpa. S.

ment of Microbio

nomycetes were. Total nineteen

production. Bolasses as the soreptomyces S2. Ece on biosurfactaNaNO3 (1g%)urification of bio

Biosurfactant, Bi

Arts, Science 11005

biology

PP6Molasses for B

by Streptom

anam, DeepalMujumdar an

smitabhuyan@

ology, Modern coShivajinagar Pu

e isolated fromn isolates were

Biosurfactant prole carbon sourceEffect of enviroant production w

supplementatioosurfactant is und

Key wo

ioemusification,

Mi

Published on 25t

69 Biosurfactantmyces S2

li S. Mane., Gand Smita. S. B

@gmail.com ollege of Arts, Scune 411 005

m soil and scrobtained, out

roduction was e. Maximum bio

onmental paramewere checked. pHon resulted in der process.

ords Actinomycetes,

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

t Production

auri M. PawaBhuyan*

cience & Comm

reened for prodof which, fivecarried out in

osurfactant prodeters such as pHH of 7.5 , Pb (0

maximum bio

Streptomyces

Conference on

1st Century February 2011 tract Book

Page 82 

ar,

merce,

duction of e exhibited n medium

duction was H, salt and 0.1g%), Mg osurfactant

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Modeand CDepa

 

Isolation

De

Indus

are dischargedTwo isolates wsupplemented guilliermondiiof phenol andmetals such aspotential in biheavy metals Plasmid was electrophoresilaboratory as to obtain highdegrade phenophenol concennitrogen sourcconsortia in im

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

n, Characteriz

Uzma T

epartment of Mic

strial wastewated into the naturawere obtained bwith phenol (1

i were selected od degradation es Ni+2 Cu+2, Pb+2

oremediation. Pland antibiotics w

isolated by is. Horizontal gwell as simulate

h degradation efol at different pHntration. Efficience (20 mg of N2/mmobilized form

Arts, Science 11005

biology

PP7zation and A

T. Momin and

crobiology, The

rs containing hial ecosystem repby selective enri00- 1000 µg/mlon the basis of grefficiency. Both2 and Cd+2 as welasmidial locatiowas detected in oalkaline lysis

gene transfer bed conditions. Thfficiency. The coH, temperature, nt biodegradation/mg of phenol) u

m also degraded p

Mi

Published on 25t

70 Application of

Varsha K. Va

institute of Scien

igh concentrationpresenting seriouichment techniqu). Both the isolarowth response t

h the strains shell as to various on of genes confeonly one isolate method and dy transformatiohe two isolates wonsortia were evorganic and inorn was observed a

urea, phenol concphenol.

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

f Phenol Degr

aidya

nce, Mumbai 32

ns of phenolic cus environmentaue using minimates, identified ato increasing conowed resistanceantibiotics indic

ferring resistanceby acridine oran

detected by agon was carried were used as a c

valuated for theirganic nitrogen at pH 7, temperacentration 1000 µ

Conference on

1st Century February 2011 tract Book

Page 83 

raders

compounds al problem.

mal medium as Candida ncentration e to heavy cating their e to phenol, nge curing. garose gel out under

consortium ir ability to source and ature 30°C, µg/ml. The

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Progressiv

Modeand CDepa

 

Antibacte

Prati

Depa

Media new anti-isanctum), gsativum), gantibacterial ain vitro antibacetone werehigh antibacte

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

erial Activity

ibha A Patil ,

artment of Micro

icinal plants areinfectious agentginger(Zingiber guava(Psidium activity .Out of tbacterial suscee used for extrerial activity aga

Solvent extrac

Arts, Science 11005

biology

PP7

of MedicinaPathog

, Shilpa S Muj

obiology, Moder

e important for ts. Plants like

officinale), lemguajava), le

these plants neeeptibility tests. raction .The ethainst Bacillus, S.

Key wocts, Medicinal pl

Mi

Published on 25t

71

l Plants againgens

jumdar, Madh

rn College, Shiva

the study of t Neem (Azard

mon(Citrus auremon grass(Cymem ,tulsi , ginge Solvents like

hanolic and ac aureus and Kle

ords lants, Antibacter

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

nst Gastroint

huri Amrutka

ajinagar, Pune 0

traditional uses dica indica ), turantifolia), gambopogon citraer ,lemon weree methanol, ethcetone extracts ebsiella.

rial activity

Conference on

1st Century February 2011 tract Book

Page 84 

testinal

ar.

05

and act as ulsi(Ocium

arlic(Allium ate) show taken for hanol and exhibited

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Progressiv

Modeand CDepa

 

Utilizatio

Envir

recognized aswhich affects soil by oil caupermeability acounter measueffective and mechanic worwere enrichedbacterial strain

Thesand biochemiOut of these 3Pseudomonasactivity had bsteel industry analyzed for Alkalinity, Chcomparison ofwhich are inibiodegrader.

Oil

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

on of PetroleuSoil and

Dalvi C. S

DNew Arts

ronmental pollus one of the momany species o

uses it to lose itsand binding capure to remediateefficient strategy

rkshops were seld using Bushnens capable of usie isolates were ical characteristi30 isolates, 7 sh, Serratia, Bacieen observed oneffluent from Aphysico-chemic

hloride and Phosf oil degrading citially present i

degradation, Lip

Arts, Science 11005

biology

PP7

um HydrocarContaminate

S., Satbhai M

Department of Ms, Commerce a

Ahmedn

ution with petrost serious problef plants and anim

s useful propertiepacity. Hence, the soil contaminay to speed up thlected where theell-Hass enrichming used engine identified on theics using Bergyhowed prominenillus, Micrococcn Egg yolk agar,Ahrnednagar MIcal characteristicsphate. Along wicapability of the in the effluent

Keywo

pase activity, Ph

Mi

Published on 25t

72

rbons by Baced With Engi

M. A., Bhutada

Microbiology, and Science Conagar

oleum and petrems. Oil is relemals as well as es such as fertilihe attempt of stuated with oil. Bhe cleanup procee soil had been cment medium fooil as a sole carbe basis of standa’s manual of Det oil degrading a

cus, Proteus and Phenol red agarIDC. (Indian Secs which includith these the furtisolates obtainedsample and pre

ords hvsico-Chemical

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

cteria Isolatedine Oil

a S. A.,

ollege,

oleum productsased into the enhumans. Contam

ity, water holdinudy was to inve

Bioremediation pess. For that purcontaminated. Soor a week. A tbon source were ard morphologiceterminative Baactivity. These id Alcaligens. Tr and oil powdereamless Metal Tde BOD, COD,ther study also ind from soil and teparation of car

characterization

Conference on

1st Century February 2011 tract Book

Page 85 

d from

s has been nvironment mination of ng capacity, estigate the provides an rpose three oil samples total of 30 isolated.

cal, cultural acteriology. isolates are

Their lipase r agar. The

Tubes) was , DO, pH, ncludes the the bacteria rrier based

n

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Modeand CDepa

 

Biodegrad

B. A. K

Insec

compounds anderived. The microorganismdegraded are persistent. Usethe entry of thsuch pesticidefrom such instheir eliminatconstructive abiodegradationpest controllerleaf-feeding pused for screesubjected to aliquid media awas investigaisolates. A comfurther intendelimination ofdegradation st

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

dation of Com

Karode, N. B.

DPSGVPM

cticide degradatind, in some cas

most commonms, especially t

called non-perse of persistent inhese insecticide

es is a major envsecticides so thetion or minimi

approach focusedn of a pesticide trs commonly us

pests on cotton, sning of organisma range of concand the colony fated to determinmparative accou

d to determine thf the supplied qtudies in the field

Arts, Science 11005

biology

PP7

mmercially U

Chaudhari, SJobanp

arpana_jl2@redDepartment of MM’s, ASC Colleg

ion is the breakises, down to then type of degrthe fungi and sistent while thnsecticides like s in the ecosyst

vironmental concre is a serious nization in the d towards envirothiamethoxam aned nowadays un

soybean etc. Serims which could dcentrations of peforming units anne the concentr

unt of the isolateshe number of daquantity of the d.

Mi

Published on 25t

73

Used Insectic

. S. Patil, P. Tputra

diffmai1.com Microbiology, ge, Shahada, 425

ing down of toxe original elemeradation is carbacteria. Insect

hose which resisMalathion, Carbtems. Contaminacern. There is a need to develop

environment. Tonmental managnd diafenthiruon

nder various tradial dilution and pdegrade these peesticides from 1

nd absorbance inrations which cs has been depicays which wouldpesticides and

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

ides by Soil I

T. Patil, and A

5409

xic pesticides intnts from which rried out in thticides which ast degradation abofuran etc, has ation of soil andpotential carcinoremediation pro

The present argement. It highlign. They are broade names for despour plate technesticides. The iso10-100 mgL’ inn liquid media recould be toleratted in the presend be required fouse the data fo

Conference on

1st Century February 2011 tract Book

Page 86 

Isolates

A. H.

to nontoxic they were

he soil by are rapidly are termed resulted in d water by ogenic risk ocesses for rticle is a ghts on the d spectrum struction of niques were olates were n solid and espectively ted by the ntation. We or complete or trails on

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Modeand CDepa

 

‘Department o

Due t

situ biodegrainevitably hasmeasured in microbial popdirect measurdegradation pamounts of thsuch methods means have fdiesel oil was isolates of soipolluted with with the unpodegradation bythus determinefrom the thirddegradation w

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

Biodegrada

V. R. Ko

of Microbiology,

to wide spread udation of pollus hazardous effe

laboratory testpulations, gravimre of bioremediaprocess aids the he oil can be clea

find limited usefound considerabmonitored over il identified as Pthe oil samples

olluted control say these isolates ed. There was ad day continuin

was investigated t

Diese

Arts, Science 11005

biology

PP7

ation of Diese

othari’ and A.

arpana_j12@red, PSGVPM’s, AS

Maharashtr

use of diesel oil,utants, has receects on human ts of bioremed

metrically measuation efficacy is

elimination of ared by various e under natural eble significance a twenty-seven Pseudomonas aeat a loading rateamples were seeat the interval o

a consistent decrng till the twento be approxima

Key Woel oil, Gravimetri

Mi

Published on 25t

74

el Oil by Soil

. H. Jobanput

diffmail.com SC College, Shahra, India

, the natural atteneived a large alife and safety.

diation efficacy uring the quantitys the monitorinspilled oil from

physical and chenvironmental cin the past few

day period usingeruginosa and P

e of 2% (v/w). Theded with the twof three days, upease in the quan

nty-seventh day. ately 54 % under

ords ic, Bioremediati

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

l Isolates

tra2*

hada, 425409, N

nuation, which ramount of atten The parameter

include enumy of residual oil

ng of diesel oil. m the environmemical methods

conditions. Microw decades. Degrg gravimetric mePseudomonas sphese soil sample

wo isolates. The p to twenty sevenntity of residual

The approximar un-optimized co

on

Conference on

1st Century February 2011 tract Book

Page 87 

Nandurbar,

relies on in ntion. This rs typically

meration of . The most Microbial

ment. Large . However, obiological radation of ethod .Two

pecies were es, together

rate of oil n days was oil starting ate rate of onditions.

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Progressiv

Modeand CDepa

 

Raw w

Ru

Dep

Wastcountries. Esbecoming anfilling, land directly into bread edgeshospitality aproper and scsource of migrowth medbiofungicide like biosolidTreatment Pdays. Waste were collecteon sterilized hrs, and obssporulation sTrichodermabiobeneficiatmethods for is most prom

Tri

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

wastes as altes

ushikesh Sank

partment of Mic

te disposal isspecially bioson environmenta

spreading, anwaste dumps f, and bread c

and hotel induscientific manneicrobial nutrienia for Trichod and promotesds were colle

Plant, Erandwapotatoes were

ed from restau raw wastes aserved for grosubstantiating a viride. Usintion of wastesmass productio

mising for susta

ichoderma viri

Arts, Science 11005

biology

PP7

ernative medustainable de

kpal*, Sunil D

rushisankpal@crobiology, Fer

s of major colids generated

al nuisance. Cod incineration.from vegetablecrumbs are distry. All theseer. The comfornts. Hence, in tderma viride. s good plant grcted from Pu

ane, Pune. Thee collected fromurants. Spores septically, incuowth. The resthat these was

ng this simpls. Moreover, ton of Trichodeinable develop

Key wo

ide, wastes, bio

Mi

Published on 25t

75

dia for Trichoevelopment

Deshmukh an

@gmail.com rgusson Colleg

oncern in devfrom waste wanventionally th. Lots of wast markets and aisposed off in wastes need

rting fact is thatthis study we uTrichoderma vrowth in many

une Municipal ese biosolids wm vegetable mof Trichodermubated at roomults showed vstes can be vele know-how this technique

erma viride. Biment.

ords

osolids, bread e

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

oderma: towa

nd Vinay Rale

ge, Pune 41100

veloped and dater treatment hey are disposeted potatoes aragricultural landn large quantito be disposedt these wastes aused these raw viride is a we

y ways. Waste Corporation’s

were sun-driedmarket while brma viride were m temperature very good groery good medi

we can brie is cheaper thobeneficiation

edges, potatoes

Conference on

1st Century February 2011 tract Book

Page 88 

rds

04

developing plants are

ed by land re thrown ds. Bread, ities from d off in a are a good wastes as

ell known materials

s Sewage d for four ead edges sprinkled for 48-72

owth with ia for the ng about han other of wastes

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Progressiv

Modeand CDepa

 

Char

*Departmen

PashRamnadi rivudrinking purpdrinking purbecause of hindustrial wecosystem isPashan lake wand to study of any aquatwill pave a wused again as

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

racterization

Rahul N

nt of Microbiol

han lake is a ulet of Mutha pose. At presenrpose because

human activitiewaste water frs under stress owater with respits microbial d

ic system and way to know ws a potable wat

Arts, Science 11005

biology

PP7

n and biodive

Nimbalkar and

ogy, Sinhgad CPune-

fresh water

river. In the pnt this water is of deteriorate

s like washingfrom nearby hof water pollutipect to its physdiversity. Physits biodiversitywhether in fututer resource or n

Mi

Published on 25t

76

srsity of Pash

d Varsha Kulk

College of Scien4 1

man made repast, water from

not used by Md water qualit of trucks and human settlemion. Present stusico-chemical asico-chemical ay are influenceure this fresh wnot?

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

han lake wat

karni

nce, Ambegaon

eservoir constrm this lake was

Municipal Corpoty. This deteridumping of se

ments. At preudy is to characand biological pand biological pd by each othewater resource

Conference on

1st Century February 2011 tract Book

Page 89 

er

n Budruk,

ructed on s used for oration for ioration is ewage and esent this cterize the properties properties

er. Results e could be

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Progressiv

Modeand CDepa

 

In

Depa

The

types of m

circulation.

sterile plast

overnight, t

mannitol sa

the basis

characteristi

found micro

species, kle

hemolytic s

ve Education society’s

ern College of ACommerce, Pune 4

artment of Microb

nfluence of

artment of Micr

present inve

microbial flora

Paper notes

tic bags, inoc

thereafter sub

alt agar, and m

of colony c

ics, Enzyme

oorganisms a

ebsiella spec

treptococci, c

Arts, Science 11005

biology

PP7f Microorga

Manjiri Mu

robiology, Prof. Science co

estigation wa

a present on

were collecte

culated in pe

bculture on di

mac-conkey ag

characteristics

reactions isol

are Staphyloc

ies, salmone

coagulase nega

Mi

Published on 25t

77 anisms on I

ukund Deshpan

Ramakrishna M

ollege, Akurdi 44

as carried out

Indian curre

ed from differ

eptone water

ifferent medi

gar and incub

s, Gram’s s

lates are iden

occi, streptoc

lla species,

ative staphylo

State Level

icrobiology in 2125th – 26th F

Abst

th February 2011 

Indian Curr

de

More Arts, Comm4

t to observe

ency (paper n

rent places of

for 5 min, i

a such as blo

bated for 24 h

staining, Bio

ntify, Some co

cocci, E-coli,

α- hemolytic

ococci etc.

Conference on

1st Century February 2011 tract Book

Page 90 

rency

merce and

different

notes) in

f Pune in

incubated

ood agar,

hours. On

ochemical

ommonly

Bacillus

c and β-

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i

Author Index 

Author Page No. Author Page No. A. H. Jobanputra 86,87 Deepali Giremar 64 A. J Joshi 74 Deepali Mane 21, 82 A. R. Desai 22 Dhutekar S.S 55 A.V. Pundle 39 Divya Nair 20 A.V.Bhosale 78 Diwan V.A 12, 30 Aasawari S. Pawar 01 G.K.Wagh 63

Abhay Raorane 02, 64 Gauri Devasthale 20 Abhijit More 09, 62 Gauri Jadhav 37 Aher N. S 77 Gauri Pawar 21, 82 Akshay Borkar 03 Ghule P.V 15 Alka Jagdale 04 Gore Suneeti 67 Alok Vyas 20 Hrishikesh D. Dhamal 05 Amar D. Jadhao 05 Hrishikesh Joshi 22 Amit Sinnarkar 63 Hussain Khokhawala 41 Anil M. Garode 06 Indulkar V.S. 23 Anita N. Katkade 11 Jadhav Sandip R 44 Anupama Sonawane 43 Jain Reena R 24 Anushka Devale 04 Jayesh A. Patil 25 Aparna Das 60 Jhilmil Ghoshal 20 Archana J. Marry 08 Jogi M.M. 25 Avinash D. Bholay 11, 13, 17 Joshi A. J 74 Avinash Sunder 39 Joshi S. A 72 Ayushi.A.Chaurasiya 07 Jyoti Mantri 08, 50 B. A. Karode 86 Kadam Shradhha D 24 B.A. Aglave 71 Kamble. A 19, 27 Balu A. Chopade 81 Kapadnis B.P 19, 27 Barbuddhe S.B. 02 Karishma R. Pardesi 40, 45, 75 Bedare Monika 10 Kaveri S. Palekar 11 Bedse,Tanuja D 44 Kavita Parekh 73 Bhadale S. V 15 Kawade S.S 30 Bhalerao M.N 15 Ketki Nalawade 28 Bhalerao V. B 77 Khadpekar A 29 Bhalkikar S.B 12 Khandelwal S.R 72 Bhavsar S. P 72 Kokani N. F. 72 Borkhataria B. V 77 Kulkarni 15 Borse Priti K 13 Kurup Sunila .B 17 Chavan K.J 16 Lalit Lande 31 D. D. Giramkar 80 Laxmi Nair 39 Dalvi, C. S 85 M. S. Vrushali 32

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ii

De Souza N.A 19, 27 Madhuri A. Udata 74 Deep.V.Barot 18 Madhuri Amrutkar 10, 65, 84 Deepak R. Patil 34 Madhuri Londhe 33 Mahesh R. Ghule 34 Priyanka Mane 49 Maid Sneha A 13 Purnima Ranawat 50 Manasi Karmarkar 35 R.B.Vaidya 07, 66 Manoj Bhosale 03 Rahatekar Rohini .R 17 Meenakshi Dhar 36 Rahul Brid 51 Meghana Kukarni 35 Rahul Nimbalkar 89 Mehta Khyati 46 Rajendra Choure 01, 28, 42, 51 Milky Sahukar 73 Rajesh M. Chomal 34 Mokshada Godbole 38 Rakhi Tari 52 Monika More 37 Rakte Snehal B 13 More Bhagyashree V. 25 Ranjekar M.K 12, 16, 23, 30,

55, 74 N. B. Choudhari 86 Ratnakaran Neena 46 N.Rutika 32 Ravi V. Kale 26 Namra Ghansar 76 Ravindra Baviskar 53 Nasim Kherad 36 Rebecca.S.Thombre 29, 56, 68 Navale S. K 72 Reena A.Dighe 54 Neelima Kulkarni 38, 41 Repe A.B 55 Neha Naik 39 Riddhi J. Shah 40 Neha S. Vora 40 Roma A Chougale 57 Nikita L. Lad 42 Rushikesh Sankpal 88 Nilesh A. Sonune 63 Rutuja.R.Phatate 56 Nilesh P. Anandwani 25 S. A. Bhutada 14, 70, 85 Niruta Y. Killedar 43 S. B. Kasture 70 P. G. Shadija 79 S. J. Ghosh 79 P.S. Patil 44 S. M. Kulkarni 72 P.T. Patil 86 S.B.Dahikar 70 Pandurang Lahare 41 S.O. Gupta 71 Pankaj .S. Picha 45 S.P.Pawar 78 Parkar S.D 19, 27 Sabah Shaikh 58 Patil Jayesh A 25 Sagar Vandra 59 Patil N.N 15 Salokhe S.G 03 Patil S. S. 86 Samriddhi R. Sharma 60 Patil S.D 16 Sandeep Gajbhiye 61 Patil Sham A 44 Sangeeta Jangam 69 Peehu S. Pardeshi 81 Saral Ghosh 54 Poojari Pritika 46 Satbhai, M. A 85 Poorva Dabir 35 Sayali Mahajani 09, 62 Pradnya Kanekar 09, 62, 63 Seema S.Sarnaik 09, 62, 63

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iii

Pradnya N. Nadivkar 47 Shahina Shaikh 64 Prajakta Bhagwat 48 Sharma L. P 72 Prakash R. Thorat 26 Sheetal .P. Pardeshi 45, 47 Pratibha A Patil 84 Shikha Malla 65 Priya Bendre 48 Shilpa Mujumdar 04, 21, 31, 33,

49, 53, 65, 82, 84

Sunny Gaikwad 53

Shilpa Sabnis 52, 58, 59 Supriya Bhopale 76 Shilpi.R.Chand 66 Supriya V. Suryawanshi 25 Shirish Shah 03 Tambekar D. H 14, 70 Shirke M. D 72 Tejlaxmi Inamdar 73 Shirode Abhinav 46 Udata M.A. 74 Shradha B. Nadhe 40 Urvi Doshi 75 Shradha Bashetti 04 Uzma T. Momin 83 Shreyas Kumbhare 41 V. R. Kothari 87 Shubhangi Puranik 68 V.A. Tile 77, 78 Siddiqui A.G 23 V.S.Vatkar 79 Singh Viveka 67 Vaidehi Dande 39 Smita A. Daule 80 Vaishali Prabhune 76 Smita S. Bhuyan 04, 21, 31, 33,

49, 53, 81, 82 Varsha Kulkarni 89

Snehal B. Bari 63 Varsha.Vaidya 18, 83 Sonal.P.Bathija 56 Vidhate Pushpa.S 17 Sonawane H.V 03 Vidya N. Udawant 80 Sourabh Mehta 68 Vijendra A. Kavatalkar 81 Srivastava Shreya 67 Vinay Rale 36, 88 Sulkshane H. C 72 Vivek N. Bobade 05 Sumedh S.Deshmukh 38, 43 Vrushali Patil 48

Sunakar 61 Vyankatesh M. Sanam 21, 82 Sunil Deshmukh 88 W.R.Sayyed 78 Sunil Tiwari 69 Yogesh S. Shouche 34

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With Be

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iv

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With Be

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plements

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With Be

vi

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plementss From 

 

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