Analysis of chemical composition of volatile substances from two

tobacco leaf species by headspace solid-phase microextraction (HS-

SPME) coupled with gas chromatography-mass spectrometry (GC-

MS) techniques was explored. The optimum conditions were the

extraction of tobacco leaf (1.000 gram) with DVB / CAR / PDMS for 30

minutes at a 50 degree Celsius. Thirty-six compounds from Burley

tobacco was found of which 22 signals were identified representing

peak for 90.31 %. The results showed that pyridine was the main

component representing 70.98 %. Chemical composition from

Virginia tobacco was found with 20 signals. Fifteen signals

representing 90.63 % were identified. The results showed that 6-

methyl-5-hepten-2-one was the main component Virginia tobacco

representing 27.84 %.

The cigarette manufacturing industry in Thailand, divided into 3

types, which are Virginia, Burley and Turkish leaves.

When the tobacco burned, will cause different chemical composition

and other properties such as smell and taste, which vary according

to the type of tobacco.

The industry Producing cigarettes is necessary to mix different

types of tobacco leaves together proportionally in order to get the

flavors satisfied by the smoker.

Analysis of chemical composition of volatile substances from two tobacco leaf species (Nicotiana

tabacum L.) by headspace solid-phase microextraction coupled with gas chromatography-mass

spectrometry techniques

ABSTRACT

INTRODUCTION

METHODOLOGY

Headspace vial1.000 g tobaccoWater bath

In 50°C for 60 mins

Saturate

SPME

AbsorbDesorb

For 30 minsEvaluate by

chromatography

GC-MS

Find the optimal condition for extraction of Burley and Virginia tobacco

RESULT AND DISCUSSION

Neophytadiene

Figure 1 The chromatogram show the ratio of

Neophytadiene

Pyridine

PyrazineSabinene

Neophytadiene

Figure 2 Chromatogram show volatile substances

from Burley tobacco

Figure 3 The chromatogram show volatile

substances from Virginia tobacco

Neophytadiene

6-Methyl-5-hepten-2-oneAcetic acid

Benzyl alcoholPhenylethyl alcohol

The analysis result of volatile substances from Burley tobacco showed that pyridine was the main

component representing 70.98 % and Virginia tobacco showed that 6-methyl-5-hepten-2-one was

the main component representing 27.84 %.The optimal condition for extract the volatile substances

from Burley and Virginia tobacco by HS-SPME coupled with GC-MS techniques, the temperature is

50 oC and the Absorption time is 30 minutes.

CONCLUSION

References1. Clark, T. J., & Bunch, J. E. (1997). Qualitative and Quantitative Analysis of Flavor Additives on Tobacco Products Using SPME−GC−Mass Spectroscopy. Journal of Agricultural and Food Chemistry, 45(3), 844–849.

2. Xiang, Z., Cai, K., et. al. (2013). Simultaneous Extraction and Derivatization of Volatile Organic Acids in Flue-Cured Tobacco with Headspace Solid-Phase Microextraction Followed by GC-MS. Analytical Letters, 46(4), 640–650.

We would like to thank Asst. Prof. Dr. Aphiwat Teerawutgulrag, Mr.Theerawat Sankumlor and

Ms. Chanidapha Thiraphatchotphum at Laboratory of chemistry building.

Department of chemistry,Faculty of science,Chiang Mai University

Acknowledgements

Table 1 % Area of Neophytadiene with the

temperature was 30, 40, 50 ˚C, respectively.

Graph 1 the graph show %Area of Neophytadiene

with the temperature was 30, 40, 50 ˚C,

respectively.

Table 2 % Area of Neophytadiene with the

adsorption time was 10, 20, 30 minutes,

respectively.

Graph 2 Show the graph %area of Neophytadiene

with the adsorption time was 10, 20, 30 minutes

Author : Ms. Thunyarat Boonapibal Student ID 590510045

Asst. Prof. Dr. Aphiwat Teerawutgulrag

Institution : Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200,Thailand

Neophytadiene

Neophytadiene

Neophytadiene

Neophytadiene

Analysis of chemical composition of volatile substances from Burley and Virginia

tobacco by HS-SPME coupled with GC-MS techniques

Thirty-six compounds from Burley tobacco was found

of which 22 signals were identified representing 90.31

% peak are. The results showed that pyridine was the

main component representing 70.98 %. Chemical

composition from Virginia tobacco was found with 20

signals.

Fifteen signals representing 90.63 % were identified.

The results showed that 6-methyl-5-hepten-2-one was

the main component Virginia tobacco representing

27.84 %

1. To Analysis of chemical composition of volatile substances from Burley

and Virginia tobacco by HS-SPME coupled with GC-MS techniques

2. To find The optimal condition to extract Burley and Virginia tobacco

OBJECTIVE

Table 3 the optimal condition