SENSITIVITAS DAN SPESIFITAS PROTEIN URIN METODE CARIK CELUP DAN ASAMACETAT 6% PADA IBU HAMIL

Siti Bandiyah1, Budi Santosa2, Tulus Ariyadi2

1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan UniversitasMuhammadiyah Semarang.

2. Laboratorium Patologi Klinik Fakultas Ilmu Keperawatan dan Kesehatan Universitas MuhammadiyahSemarang.

ABSTRAK

Metodecarikcelupdigunakankarenapraktisdancepat,meskiseringkaliadaketidaksesuaianhasilantaracarikcelupdanmetodeasamasetat 6%. Carikceluphanyasensitive terhadap albumin saja,globulin-globulin termasuk protein Bence Jonestidakdapatdinyatakan.Selainitupengaruhkonsentrasigaram-garamdalamurindankeasamanurinberbedauntukteskekeruhandantesmemakaicarikcelup.Pemeriksaanprotein urindenganasamAsetat 6% cukuppeka, karena 0,004% protein dinyatakandengantesini.Tesinilebih sensitive jikauntukmemeriksa albumin, peptondan protein Bence Jones. Pemeriksaanprotein urin di PuskesmasNgesrepmenggunakanmetodecarikcelup,tetapiseringkaliklinisimemintauntukkonfirmasidenganmetode standard, antara lainmetodedenganpemanasanasamasetat 6%.Tujuanpenelitianuntukmengetahuisensitifitasdanspesifitasprotein urinmetodecarikcelupdanasamasetat6% padaibuhamil.Jenispenelitiananalitikpendekatan cross sectional. Hasilpenelitianterhadap 33sampelpenelitian protein urincarikceluphasilnegatif 17 sampel, positif 1 sebanyak 10 sampel, positif 2sebanyak 4 sampel, danpositif 3 sebanyak 2 sampel.Protein urinasamasetat 6%, hasilnegatif 20sampel, positif 1 sebanyak 9 sampel, danpositif 2 sebanyak 4sampel.Ujisensitivitascarikcelupmempunyaisensitifitascukupbaik (85%) danspesivitas yang tinggi(100%).

Kata kunci : protein urin, carikcelup, asamasetat, sensitifitas, spesivitas

http://repository.unimus.ac.id

THE SENSITIVITY AND SPECIFICITY OF URINE PROTEIN WITH DYE-DIP AND6%ACETAT ACID METHOD ON PREGNANT WOMEN

Siti Bandiyah1, Budi Santosa2, Tulus Ariyadi2

1.Study Program of D IV Health Analyst Faculty of Nursing and Health Sciences University ofMuhammadiyah Semarang.

2. Laboratory of Clinical Pathology Faculty of Nursing and Health Sciences University ofMuhammadiyah Semarang.

ABSTRACT

The dye-dip method is used because it is practical and fast, although there is often a mismatch betweenthe dye-dip and the 6% acetic acid method. Dye-dip is only sensitive towards albumin alone, globulin-globulin including Bence Jones protein can not be expressed. In addition, the effect of urine saltconcentration and urine acidity is different for turbidity test and dye-dip test. Examination of urine proteinwith 6%acetic acid is quite sensitive, since 0.004% protein is expressed by this test. This test is moresensitive in case to check albumin, pepton and BenceJonesprotein. Examination of urine protein onPuskesmasNgesrep is using dye-dip method, but often the clinician ask for confirmation by standardmethod, for example method with 6% of acetic acid heating. The research goal is to know the sensitivityand specificity of urine protein with dye-dip and 6%acetic acid method on pregnant women. Theresearchtype is analytical with cross sectional approach. The researchresult towards 33 research samplesof dye-dip urine Protein was 17 samples of negative result, positive 1 as much as 10 samples, positive 2as much as 4 samples, and positive 3 as much as 2 samples. Acetic acid urine protein was 6%, 20 samplesof negative result, positive 1 as much as 9 samples, and positive 2 as much as 4 samples. The dye-dipsensitivity test has good enough sensitivity (85%) and high specificity (100%).

Keywords: urine protein, dye-dip, acetic acid, sensitivity, specificity

http://repository.unimus.ac.id