acid electroforesis

HbF HbC HbS Acid Hemoglobins Electrophoresis Kit The Microgel / INTERLAB G26 Acid Hemoglobins Electrophoresis method provides a new level of total automation on agarose gel. You simply pipette the hemolysates into the sample wells, put the gels in the holders, start the instrument and WALK AWAY! KIT REF. SRE605K (130 tests) HbA Synthesis of hemoglobin is under genetic control and the presence of abnormal hemoglobins is associated with functional, physical and morphological abnor- malities of the red cells. Hemoglobins are composed of polypeptide chains of globin and iron proto- porphyrin heme groups. Normal hemoglobins have similar structures with a molecular weight of about 67,000 daltons and consist of 2 pairs of globin chains each associated with one molecule of heme. Hemoglobin A polypeptide chains 2 a and 2 b Hemoglobin A 2 polypeptide chains 2 a and 2 d Hemoglobin F polypeptide chains 2 a and 2 g Normal adult hemoglobins are HbA (98% of total hemoglobin) and small amounts of HbA 2 and HbF. Substitution of aminoacids in the polypeptide chains sequence leads to the formation of abnormal hemoglobin variants. At present over 600 structural hemoglobins variants are known: HbS, HbC, HbE, HbD, HbG, HbH, HbI, Hb Lepore, etc. Hemoglobinopathies are a group of diseases caused by the presence of abnormal hemoglobins. About 60% of abnormal hemoglobins have a sufficiently altered charge distri- bution to be identified by electrophoresis. The variants most frequently observed are HbS and HbC. Concomitant presence of HbA and another variant (HbS, HbC, HbE, etc.) is referred to as heterozygous hemoglobinopathy AS, AC and AE respectively. The presence of only one single type of hemoglobin variant is called homozygous hemoglobinopathy, like HbS or HbC. These abnormal variants are genetically transmissible. Homozygous hemoglobinopathies may cause serious clinical effects. Acid hemoglobin electrophoresis on agarose allows confirmation of the presence of HbS or HbC already detect- ed by Alkaline Hemoglobins electrophoresis.

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HbF

HbC

HbS

Aci

d H

emo

glo

bin

s E

lect

rop

resi

s K

The Microgel /INTERLAB G26 Acid HemoglobinsElectrophoresis method provides a new level of total automation on agarose gel.

You simply pipette the hemolysates into the samplewells, put the gels in the holders, start the instrumentand WALK AWAY!

KIT REF.SRE605K (130 tests)

HbA

Synthesis of hemoglobin is under genetic control and the presence of abnormalhemoglobins is associated with functional, physical and morphological abnor-malities of the red cells.Hemoglobins are composed of polypeptide chains of globin and iron proto-porphyrin heme groups.Normal hemoglobins have similar structures with a molecular weight of about67,000 daltons and consist of 2 pairs of globin chains each associated with onemolecule of heme.

Hemoglobin A polypeptide chains 2 a and 2 bHemoglobin A2 polypeptide chains 2 a and 2 dHemoglobin F polypeptide chains 2 a and 2 g

Normal adult hemoglobins are HbA (98% of total hemoglobin) and smallamounts of HbA2 and HbF. Substitution of aminoacids in the polypeptidechains sequence leads to the formation of abnormal hemoglobin variants. Atpresent over 600 structural hemoglobins variants are known: HbS, HbC, HbE,HbD, HbG, HbH, HbI, Hb Lepore, etc.Hemoglobinopathies are a group of diseases caused by the presence of abnor-mal hemoglobins.About 60% of abnormal hemoglobins have a sufficiently altered charge distri-bution to be identified by electrophoresis. The variants most frequentlyobserved are HbS and HbC. Concomitant presence of HbA and another variant (HbS, HbC, HbE, etc.) isreferred to as heterozygous hemoglobinopathy AS, AC and AE respectively. The presence of only one single type of hemoglobin variant is called homozy-gous hemoglobinopathy, like HbS or HbC. These abnormal variants are genetically transmissible. Homozygous hemoglo-binopathies may cause serious clinical effects. Acid hemoglobin electrophoresison agarose allows confirmation of the presence of HbS or HbC already detect-ed by Alkaline Hemoglobins electrophoresis.

www.interlab-srl.com

KIT CONTENT

Gel Plates 10Blotting Paper 10Buffered Sponges 20Acid Blue Stain 1 Applicator Washing Sol. 1Lysing Solution 1Disposable Sample Plates 10

REAGENT PREPARATION

Reagents are ready to use, only the Stain has to be reconstituted with900 ml of distilled water. All may be stored at room temperature.

SAMPLE PREPARATION

After the red blood cells (RBC) are washed they are lysed as follows: 50 ml of packed washed RBC + 450 ml of lysing solution.

SAMPLE STORAGE and STABILITY

WWhhoollee bblloooodd:: 1 week at 2 to 8°C.HHeemmoollyyssaattee:: 12 hours at 2 to 8°C.

PERFORMANCE CHARACTERISTICS

AccuracyA total of 31 normal and abnormal specimens were tested with Interlab systems versus a commercial agarosesystem.The Interlab Acid Hemoglobin kits demonstrated equivalent band patterns with no false negative or false posi-tive bands. This study yielded a 100% agreement with the reference method for the observed bands.

Within Run Precision3 samples were run on 3 different gels. Each sample was run 13 times on the same gel.In each lane the bands were correctly identified.

Between Run Precision4 samples were run on 10 different gels.In each lane the bands were correctly identified.

Via Rina Monti n. 26, 00155 Rome, ItalyTel: +39-06-22754350 Fax: +39-06-22754534

E.mail: [email protected] Web Site: www.interlab-srl.com

Position of hemoglobin bandson Acid Hemoglobin gel

HbCHbS

HbA

HbF

Anode

Normal

HbCHbSHbAHbF

HbSHbAHbF

Normal HbAHbF