acquity uplc systems with 2d technology solves key challenges
TRANSCRIPT
©2011 Waters Corporation 1
ACQUITY UPLC Systems with 2D Technology Solves Key Challenges
Increase Selectivity & Sensitivity
Eliminate Unwanted
Interferences
Mobile Phase Flexibility
Characterize the Most Complex Samples
©2011 Waters Corporation 2
Complicated and Difficult to Operate Customer Enabled 2D System
©2011 Waters Corporation 3
Simple to Use and Easy to Implement, Robust ACQUITY UPLC with 2D Technology
©2011 Waters Corporation 4
All ACQUITY UPLC Systems 2D Capable New Systems and Upgrades Shipping Now
ACQUITY UPLC H-Class & Bio System with 2D Technology
ACQUITY UPLC I-Class System with 2D Technology
ACQUITY UPLC System with 2D Technology
©2011 Waters Corporation 5
Trapping — Increase Sensitivity by Loading
More Sample
Heart Cutting — Increase Resolution by
Incorporating Orthogonal Chemistries
At-Column Dilution (ACD) — Enables Large Volume Injections
of Samples in Strong Solvent
Parallel Column Regeneration — Increase Throughput
Designed for Four Functions
©2011 Waters Corporation 6
Filter Trapping of Phospholipids
desired peptides (co-eluting)
phospholipids interference
Typical LC Separation ACQUITY UPLC with 2D Technology
desired peptides
no phospholipids
Data Generated by PPD Inc.
©2011 Waters Corporation 7
Customer Success Utilizing 2D LC
PPD in Richmond, VA has over 50 LC/MS/MS Instruments all Performing 2D LC
80% of the 2D LC Analysis are “Filter” Trapping for Phospholipid removal
—Provided 25-50% Increase in Column Lifetime
“The ability to perform two dimensional UPLC separations using Waters technology has enabled some of our most challenging assays that require ultra-high sensitivity while maintaining excellent ruggedness and instrument up-time.“
- Rand Jenkins, PPD
©2011 Waters Corporation 8
2D for Heart Cutting
Blank 100pg/mL
©2011 Waters Corporation 9
2 Dimensional Dried Blood Spot Analysis of Rosuvastatin by At Column Dilution
No “At Column Dilution” With “At Column Dilution”
• Direct injection of methanol extract from DBS sample • 80uL injection of methanol • At column dilution
©2011 Waters Corporation 10
Application Information
Waters Posters — Biopharma
o Quantitation and MS Analysis of Monoclonal Antibodies in a Complex Mixture
— Bioanalysis
o Eliminate Dry Down and Reconstitution, Increase Sensitivity and Elimination of Interferences with Heart Cutting and At-Column Dilution Analysis of Dried Blood Spot Samples
Customer Generated Material
©2011 Waters Corporation 11
Trapping Installation Test Created for Installation and Support
2D UPLC System Installation Test — Perform Standard System Installation Test
— Perform 2D System Installation Test for Trapping and Elution
o Analyze % Recovery 2D Compared to 1D
2D Training Classes Available on Portal
©2011 Waters Corporation 12
Software compatibility
For the moment only supported in Mass Lynx
©2011 Waters Corporation 13
2D UPLC Systems and Upgrades are Shipping Now
MassLynx Software version 4.1
I-Class 2D Part Numbers
ACQUITY UPLC 2D Upgrade Part Numbers
P/N Description Components
176015076 I-Class FTN with 2x BSM FTN, BSM, BSM and CM-A
176015077 I-Class FTN with QSM/BSM FTN, QSM, BSM and CM-A
P/N Description Components
176002491 ACQUITY UPLC 2D Upgrade BSM and CM-A