Active Solvent Modulation

2D-LC

2

GPC GPEC(gradient polymer elution chromatography)

GPC GPEC

GPC GPEC 2D-LC

GPC GPEC

1 GPEC 2 GPC 2D-LC1 GPC

2 GPEC GPC 2D-LC GPC

Active

Solvent Modulation ASM HRS

2D-LC

Key words 2D-LC GPC GPEC

Authors

Agilent 1290 Infinity 2D-LC Solution

1290 Inifinty G7104A

1290 Inifinty G7120A

1290 Inifinty G7167B

1290 Inifinty G7116B

1290 Inifinty G1170A

+ ASM G4243A

+ MHC G4242A

1290 Infinity (ELSD) G7102A

1D GPC

PLgel MiniMIX C, 4.6 250 mm PL1510-5500

THF

0.1 mL/min

40 ,

10 L

2D GPEC

YMC Triart C18 ExRS, 3.0×150 mm 3 m

A: B; THF

25 %B 0 min 25 %B 2 min

100 %B 12 min 100 %B 20 min

0.5 mL/min

40 L

25 6

ELSD, 50

80

1.3 SLM

PS Mw; 30,000 PL2013-2001

PMMA Mw; 30,000 PL2023-2001

Poly (styrene-block-methyl methacrylate)

average Mn 30,000, block ratio of 1:1 (Sigma-Aldrrich

749184

THF

Active Solvent Modulation

1D 2D

2D

Active solvent modulation ASM 1D

2D 2D

1D 2D

ASM

ASM ASM

MHC

HRS 2D-LC

1 ASM

MHC HRS 2D-LC

2 MHC 2D-LC

MHC 2D-LC 10 1D

1D

1D

2D-

LC

2D

3 HRS 2D-LC

2D

ASM

+1D

2D

1D

2D

1D GPC

4 PS PMMA PS-PMMA block co-plymer GPC

4 PS PMMA PS-PMMA block co-plymer GPC

5 3 GPC

21.5 min 25 sec

6 GPEC

5 GPC

2D GPEC

GPEC 6

6 GPEC

GPEC PMMA PS-PMMA block co-plymer PS

GPC

6 5

HRS 2D-LC

1

ASM 2D

7 ASM

ASM

7 ASM

Agilent 1290 Infinity 2D-LC Solution

2D-LC

ASM

GPEC

2D-LC

MHC

HRS

→ 2D

2D

2D 30

2D

D 2D

1D 2D

D

( µL)

2D

( 4 ~ 320 µL)

D

( µL)

1

1D

1

1D

1

1D

SW

1. Characterization of Styrene-Acrylonitrile Copolymers

Using Comprehensive 2D-LC Investigation of Two

Mutually Dependent Distributions”

5991-6699EN

2. ELSD

GPC GPEC

LC-201807HK-002

www.agilent.com/chem/jp

0120-477-111

email_japan@agilent.com

© Agilent Technologies, Inc. 2018

Printed in Japan, October 30, 2018

LC-201810KG-001