Hepabial +Add the missing piece to

the liver’s metabolism

32

Add the missing piece tothe liver’s metabolism

Hepabial+ ROLE

The liver is the metabolism central laboratory in animals. Its reserve capabilities are absolutely essential in animals with high production level.

To offset these different critical stages during the rearing process, it is necessary to provide support for the animal’s liver and kidney functions.

A unique combination of ingredients selected to stimulate energy metabolism and to optimize overall performances of animals.

Signs observed :

­ Reduced appetite Drop in laying rate Reduced immune defences Reduced ADG Stress sensitivity Increased mortality

CONSEQUENCES OF LIVER DYSFUNCTION

L-carnitine(10 g/L)

Transports long chain fatty acids

Allows storage / release of fats in case of high energy needs

Choline(150 g/L)

Allows bile to ensurefat digestion

Helps with fat digestion

Lipotropic factors**

An original targeted formula

Liver toxicity

Mobilisation of reservesmetabolised by the liver

Kidneytoxicity

Improved energy metabolism

Special features of Hepabial+ * Diuretic: substance which leads to increased urine secretion ** Lipotropic: substance which adheres to fat or facilitates its transformation*** Choleretic: substance which facilitates bile secretion, cholagogic: substance wich facilitates bile evacuation

Supplementing the diet with Hepabial+ makes it possible to overcome the energy deficiency linked to all liver dysfunctions.

Better performing animals Improved ADG and FCR Optimized reproductive performances Reduction of fatty liver syndrome

Carbohydrate metabolism(blood sugar regulation)

Iron and vitaminstorage

Fat metabolism (Fatty acid synthesis,fat storage control)

Bile secretion(helps with lipids and

carbohydrates absorption)

Protein metabolism(Protein and urea synthesis)

Cholagogic andcholeretic*** factors

Sorbitol (400 g/L)

Encourages bileproduction and secretion

Stimulates liver activity

Helps with fat digestion

Artichoke Black radish

FumitoryDandelion

Stimulates appetite Encourages bile secretion Stimulates toxins elimination

Diuretic* andstimulant factors

Hepabial+

=

The liver

Hepabial+ Hepabial+

TRIAL N°1 TRIAL N°2

PROTOCOL PROTOCOL

RESULTS RESULTS

CONCLUSION

CONCLUSION

2 buildings with 21300 one-day-old broiler chicks in each one Control group : no supplement Treated group : 1 ml of Hepabial+ / L of drinking water for 2 days in every feed change periods

4 buildings with a total of 99 500 one-day-old broiler chicks 2 control buildings : no supplement 2 treated building : 1 ml of Hepabial+ / L of drinking water for 2 days in every feed change

periods

The calcultation of breeder’s economic results for his two buildings shows a net margin improvement* of 6% with Hepabial+ at each feed change period.

On average, supplementing broilers with Hepabial+ at feed change periods allows to improve breeder’s margin of more than 28 % thus an economic gain of 1.40€ / m2.

* net margin = income - (feed cost + chick cost + Hepabial+ cost + other costs)

* net margin = income - (feed cost + chick cost + Hepabial+ cost)

+ 6 % net margin* for breeder

- 27 % mortality

+ 28 % margin* thus +1.4 € / m2

- 15 % mortality

Effect of Hepabial+ supplement on mortality, average daily gain and body weight (trial august 2008). Effect of Hepabial+ supplement on feed conversion ratio, mortality, average daily gain and body weight.(trial august 2008)

Control group

440

55.1

2.07

Control group

1.98

504

52.6

2.12

Mortality

ADG (g)

Body weight (kg)

FCR

Mortality

ADG (g)

Body weight (kg)

Treated group

320

57.1

2.15

Treated group

1.83

429

52.1

2.11

Evolution

-27%

+3.6%

+3.9%

Evolution

-7.3%

-14.88%

-0.94%

-0.47%

54 Hepabial+

Efficacy on broilers at feed change periods Efficacy on broilers at feed change periods

Hepabial+

Increased body weight gain : + 3,3% Decreased feed conversion ration : - 6,7% Improved liver function

Improved immune response

PROTOCOL

360 broilers divided into 6 groups

Dosage : 1ml of /L of drinking water for the first 3 days of life then 3 days at each feed change period (D1-D3, D15-D17, D29-D31)

Hepabial +

Group 1 Group 2 Group 3 Group 4 Group 5 Group 6

Hepabial+ - + - + - +Aflatoxine B1 - - + + - -Ochratoxine A - - + + + +

RESULTS

1) Zootechnical performances

2) Blood parameters (levels of liver enzymes and bile acids)

3) Immune response (Newcastle vaccine)

Vaccination at the hatchery and on day 9

Compensastes for loss of growth caused by the mycotoxins Reduces feed consumption even with uncontaminated feed Supplementing the diet with Hepabial+ improves the feed conversion ratio

Better liver function

Higher antibodies titres in group treated with Hepabial+ Aids of immune response

2600

2620

2640

2660

2680

2700

2720

2740

2760

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Body weight gain (g)

+ 0.64 %+ 3.26 %

+ 2.85 %

3750

3800

3850

3900

3950

4000

4050

4100

4150

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Feed consumption (g)

- 4.55 % - 3.87 %

- 4.18 %

1.3

1.35

1.4

1.45

1.5

1.55

1.6

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Feed conversion ratio

- 5.23 %- 6.69 % - 6.87 %

Control group Hepabial+ group

0

50

100

150

200

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Blood AST* concentration (U / L)

- 5.29 % - 11.85 % - 25.71 %

0

1

2

3

4

5

6

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Blood bile acids concentration(µmol/L)

+ 9.16 %- 41.25 %

- 48.5 %

0

0.5

1

1.5

2

2.5

3

3.5

4

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

Blood ALT** concentration (U / L)

- 6.1 % - 5.9 %

- 31.77 %

Control group Hepabial+ group

0

Antibodies titres ELISA** (S/P)Antibodies titres HI* (log2)

2

4

6

8

0

0.2

0.4

0.6

0.8

1.2

1

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

No mycotoxinsAflatoxin and

ochratoxin Ochratoxin

+ 29 %

+ 5 %

+ 14 %

+ 16 % + 45 %

+ 18 %

+ 44 %

+ 20 % + 9 % + 26 %

+ 2 %

+ 48 %

Control group D9 Hepabial+ group D9

Control group D42 Hepabial+ group D42

* AST : Aspartate Aminotransferasee** ALT : Alanine Aminotransferasee

Indicators showing hyper-solicitation of the liver

{

* HI : Haemagglutination inhibition test** Elisa : Enzyme linked immunosorbent assay

CONCLUSION

Hepabial+ supplement counteracts the negative effects of mycotoxins

76

Efficacy of Hepabial+ with broilers whose feed is contaminated with mycotoxins

Hepabial+ Hepabial+

Laying curve maintained above strain standard : + 5% egg production during treatment period

Reduction of feed consumption : -6% during the period

Appetite remains unchanged during product distribution

Improvement in liver function

Improved appetite

Improved digestibilityPROTOCOL

Evaluating the benefits of a Hepabial+ supplement in hens during laying 1 test building : 31 950 laying hens treated. 4 treatments (3 to 4 weeks apart) Dose : 1ml / l of drinking water for 5 days. Treatment 1 : W 58 / Treatment 2 : W 61 / Treatment 3 : W 64 / Treatment 4 : W 68

Hepabial +

95

90

85

% la

yin

g

80

75

70W40 W42 W44 W46 W49 W50 W52 W54 W56 W58 W60 W62 W64 W66 W68 W70

120

115

105

100

110

95

90

85

80

Batch treated Standard Feed ingested

ing

este

d(g

)

RESULTS

20

40

60

100

Blo

od

hep

atic

en

zym

eco

nce

ntr

atio

n (I

U /

L)

80

0Before treatment After treatment

-30%

13,27 6,14

81,43

56,6

ASAT

ALAT

-54%

Effectiveness of Hepabial+ at 70-80 days in beef calves during the slowdown in consumption

PROTOCOL

SYSTEMATIC TREATMENTS WITH HEPABIAL+

RESULTS

304 calves treated (2 batches)Distribution of 5ml of per feed per treated calf for between 5 and 70 days.The following were noted : Calves’ appetite Digestibility (consistency of feaces) Blood parameters (ASAT, ALAT)

Hepabial+

Start : added to milk to improve consumption• 5-7 day treatment

At the 70-80 day period : higher concentrate in feed : calves full up• 8-10 day treatment

Isolated treatments : unexpected drop in consumption (heat)• 2-3 day treatment

- 30 % on ASAT level (Aspartate Aminotransferase)

- 54 % on ALAT level (Alanine Aminotransferase)

Indicatorsshowing

liver pain

Effects of Hepabial+ on blood levels of hepatic enzymes

98

Effectiveness of Hepabial+ in laying hens

Hepabial+ Hepabial+

Effect of supplementing sows with Hepabial+ at birth

Effect of supplementing sows with L-carnitine during pregnancy and lactation

+ 6% body weight gain of piglets ( statistically significant difference)

Improved reproductive performancesFaster growth of piglets

PROTOCOL PROTOCOL

86 sows in trial, divided into 2 groups : Control group : no supplement (30 sows) Treated group : either 1 ml of Hepabial+ /L of drinking water during the 5 days after birth (37 sows)

or 1 ml of Hepabial+ /L of drinking water during 5 days at the 3rd week of lactation (19 sows)

The two treated groups are not differentiated because there is no statistically significant difference between them.

127 sows in trial of which 40 gilts. All the animals are divided into 2 groups : Control group : no supplement Treated group : 125 mg of L-carnitine / day / sow during pregnancy

250 mg of L-carnitine / day / sow during lactation

1

34

2

5

8

7

6

0Birth body weight Weaning body weight

7,13

1,77

6,85

1,91

Treated group Control group

RESULTSRESULTS

Evolution of piglets average body weight (trial 2008)

Performances evolution of treated group in comparison with control groupEvolution of treated group performances in

comparison with control group (trial 2008)

Control group

12.8

1.9

6.9

5

Number of piglets / litter

Body weight of piglets at birth

Body weight of piglers at weaning

Body weight gain of piglets during

lactation

Treated group

12.6

1.8

7.1

5.3

P

NS

NS

NS

*

* Represents statistically significants effects : p<0.05 NS : no significatif : p>0.05 +6%

Litter weight at birth

+8%

+7%

Gilts

Sows

Piglets weight at birth

+9%

+6%

Body weight gain during lactation

+12%

+4%

Litter weight at weaning

+14%

+10%

CONCLUSION

CONCLUSION

Supplementing with Hepabial+ after birth allows to support sows appetite, a better use of feed, leading to a faster growth of piglets.

A supplementation with L-carnitine during pregnancy and lactation periods improves reproductive performances of sows and allows a faster growth of piglets.

Differences between the control and the treated groups are more significant with gilts.

Source : Ramanau A, Kluge H, Eder K, J Anim Nutr 2001Source : Ramanau A, Kluge H, Spilke J, Eder K, J Nutr 2004

1110 Hepabial+ Hepabial+

Vers

ion

02/

2016

Biolink Limited,Halifax Way, Pocklington Ind. Est., Pocklington, York YO42 1NR. United Kingdom.Tel: +44 (0) 1759 303444 - Fax: +44 (0) 1759 303158Email: info@biolinklimited.co.uk - Web: www.biolinklimited.co.uk

Hepabial+ The personalized solution

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Broilers X X X X X X X

Turkeys X X X X

Pullets X X

Laying hens X X

Forced-feeding ducks X X X

Muscovy ducks X X X X X X

Lactationpreparation

(before giving birth)

Lactation peak(ingestionsupport)

Feed transition

Heat stroke

Breeders X X X

Fattening rabbits X X

Weaning AcetosisFeed

blockageHeat stroke

Hepatic overload

after treat-ment

Fattening calves X X X X

Cattle X X

WeaningLactationsupport

Feed blockage Heat stroke

Sows X X

Piglets X X

Fattening pigs X X

Instructions for use :Hepabial+ is used diluted in drinking water.Poultry : 1 ml / L of drinking water for 5 to 10 daysCalves during fattening : 1 ml / L of liquid feed for 5 to 10 daysOther species : 1 ml / L of drinking water for 5 to 10 days