advances in understanding ambrosia beetle chemical ecology
TRANSCRIPT
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Jason Oliver1, Chris Ranger2, Mike Reding2, and Pete Schultz3
1 Tennessee State University, School of Agriculture and Consumer Sciences,Otis L. Floyd Nursery Research Center, McMinnville, TN
2 USDA-ARS, Horticultural Insects Research Laboratory, Wooster, OH3 Virginia Tech, Hampton Roads Center, Virginia Beach, VA
Advances in Understanding Ambrosia Beetle Chemical Ecology and Utilizing the Findings to Improve Insecticide Management
2.0 mmUSDA-ARS, Wooster
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Outline
1) General Biology and Information
2) Research
3) Management Issues / Options
TrappingInducing Tree AttacksInsecticide StudiesPlant Stress Studies
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Subfamily Scolytinae
Ambrosia, Bark Beetles, and Engravers
~ 5,812 species worldwide
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- Many are attracted to volatiles probably related to plant stress (e.g., ethanol)
Ethanol Lure Pack
Theysohn (“slot”)LindgrenFunnel
Vane Panel
- Dark silhouettes
Ambrosia Beetles
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24 Species Trapped at Tennessee Nursery Center (1998 / 1999)
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Granulated (Asian) Ambrosia Beetle(Xylosandrus crassiusculus [Motschulsky])
Actual Size
Lesser Shothole Borer, Fruit-Tree Pinhole Borer(Xyleborinus saxeseni Ratzeburg)
Black Stem Borer, Smaller Alnus Beetle, Tea Root Borer(Xylosandrus germanus Blandford)
Species Attacking Nursery Stock in Tennessee
Thysanoes fimbricornis LeConte
2.0 mmUSDA-ARS, Wooster
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- Numerous reports of damage across eastern U.S.
- Introduced to South Carolina in 1974
- Now widely distributed
Granulated Ambrosia Beetle (“Asian AB”)
5 – 10 GAB will kill most trees under 3 inch caliper (Mizell and Riddle 2004)
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- Seems to be more problematic in northern states
- Introduced at Long Island New York about 1932
- Now widely distributed
Black Stem Borer
- Multiple reports of being problematic in walnut production
2.0 mmUSDA-ARS, Wooster
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CherryChestnutElmMapleOakPersimmonSweetgumBuckeyeCrape myrtleDogwoodFig
Golden raintreeMagnoliaPeachPearPecanPersimmonPlumRedbudStyraxSweetgum
Major HostsCherryChestnutElmMapleOakPersimmonSweetgumAlderAppleBeechCypress
GrapeHickoryHornbeamMulberryPineRhododendronSpruceTulip poplarTupeloWalnut
Major Hosts
Over 100 known hosts Over 34 plant genera hosts
Walnut???
2.0 mmUSDA-ARS, Wooster
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Lesser Shothole Borer, Fruit-Tree Pinhole Borer(Xyleborinus saxeseni Ratzeburg)
Black Stem Borer, Smaller Alnus Beetle, Tea Root Borer(Xylosandrus germanus Blandford)
Ambrosia Beetles Reported as Problematic on Walnut
Pear Blight Beetle, European Shot-Hole Borer(Xyleborus dispar [Fab.])
Cosmopolitan Ambrosia Beetle, Black Twig Borer(Xyleborus ferrugineus [Fab.])
Oak Timber Beetle(Xyleborus xylographus [Say])
J.R. Baker & S.B. Bambara,NCSU, Bugwood.org
Pest & Disease Image Library, Australia, Bugwood.org
Pest & Disease Image Library, Australia, Bugwood.org
(Solomon 1995)
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Importance: Most have broad host ranges, primarily preferring hardwoods
Range: Most introduced from Asia or Europe and occur over most of U.S.
Habits: Adults usually over-winter in galleriesFemales mate before leaving galleries. Males do not fly.Bore into sapwood, usually not the heartwood.Galleries consist of branches or enlarged brood chambersLarvae and adults feed on symbiotic ambrosia fungusAdults “culture” fungi along gallery wallsSome continue to extend galleries over timeSome begin galleries and are then joined by others of their speciesSome have one generation per year and others have multiple
Peak Activity: Emerge as early as February (X. saxeseni), but usually peak emergence is March through May
Host Preference: Unthrifty, injured, and dying trees. Moisture contentimportant. Attracted to stressed trees.
Generalities Among Walnut Attacking Ambrosia Beetles
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Damage: Prefer to attack before “bud-break”Wilting, flagging, branch dieback, basal sprouts, tree deathLumber staining and reduced quality from galleriesPossible vectors of other pathogenic fungi
Generalities Among Walnut Attacking Ambrosia Beetles
Control: Natural enemies (predaceous beetles / true bugs)Cultural practices promoting healthy treesPrompt harvest and use of timberSeed sources listed as important for X. germanus (Weber)Plantings near water sources may be more susceptibleTraps to time judicious insecticide treatments
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Shot holes
- Adult boring produces “shot holes”
- Boring activities can result in “sawdust” or “toothpick” like particles
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- Tunnels (galleries) generally made deep into sapwood
1 inch
Granulated Ambrosia Beetle Gallery
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- Adults carry species-specific symbiotic “ambrosia” fungus in mycangia
Doug Stone, Miss. St. Univ.,Bugwood.org
Jack C. Nord, USDA For.Serv.www.forestryimages.org
- Larvae and adults eat ambrosia fungus
Doug Stone, Miss. St. Univ., Bugwood.org
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- Female progeny emerge from host trees and fly to new host trees
- Males do not fly, but will exit trees
- Ambrosia beetles are more serious nursery and landscape peststhan other scolytids
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- Attack living, recently killed trees, or dead trees (high moisture content)
Dead tree in river under attack
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ResearchTrap Studies
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1) Used to direct scouting activities (esp. when sprays not made)
2) Used to direct timing of spray treatments
Value of Traps
Ambrosia beetles:Over-winter as adultsNo temperature dependent larval development period in springRemain inactive until conditions favorableEmerge suddenly in large numbersGenerally attack before bud breakHowever, emergence can vary widely between years and locations
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Bee
tles
Cap
ture
d P
er D
ay
0
10
20
30
40
50
60
70
80
60 80 100 120 140 160 180 200
Site-1
Site-2
2007
0
1
2
3
4
60 80 100 120 140 160 180 200
Site-1
Site-2
2006
Apr May Jun JulMar
0
0.2
0.4
0.6
0.8
1
1.2
1.4
60 70 80 90 100 110 120 130 140 150 160 170 180
Site-1
Site-2
0
2
4
6
8
10
12
60 70 80 90 100 110 120 130 140 150 160 170 180
Site-1
Site-2
2007
2006
Apr May JunMar
2006 2006
2007 2007
Site 1Site 2
- Peaks at different times in different years- Within years, similar between sites and X. crass and X. germ
Xylosandruscrassiusculus
Xylosandrusgermanus
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3) Extension assistanceWidely-spaced Contiguous
Procoxae
Xylosandrus Other
Identifying the Granulated (Asian) Ambrosia Beetle
1) Color – Reddish-brown to orange-brown(may appear two toned)
2) Non-shiny patch on abdomen tip(visible with 10x hand lens)
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Lindgren Trap
Chestnut Trees with Gallery Cages
Trapping / Tree Attack Study (1999)
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Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Jan
Tota
l Num
ber
0
25
50
75
100
500
550
600
Tree AttacksTrap Collections
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Other Findings During Study- More galleries on southwest side of tree
X. germanus – 195.2 + 13.1X. crassiusculus – 238.6 + 22.0
- Females vs. Males emergingX. germanus – 16 : 1X. crassiusculus – 15 : 1
Males did emerge
- Mixed species emergence from same gallery
- Emergence occurred in spurtsX. germanus – Up to 7 events from 54 to 89 daysX. crassiusculus – Up to 4 events from 55 to 94 days
- Greater progeny production by X. crassiusculusX. germanus – 4.4 + 0.5 per galleryX. crassiusculus – 9.9 + 4.0 per gallery
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Trap Features Evaluated:
Trap typeLure typeKilling agentsCostEase of use
Ambrosia Beetle Trap Comparisons (2003)
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X.crass
X.germ
X.sax
Species
Traps With Greater Collections Than a Non-Baited Lindgren Trap
All baited with pill bottle ethanol lure
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- Traps with highestcapture rates
- Bottle trap was best all around trap- Cheapest to make- Easiest to operate- Effective on problem ambrosia beetles
Improved- Best lure (pill bottle with wick)had highest ethanol release rate)
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Trap Height Study – Bottle Trap (2006 - 2008)
3 m (9.8 ft)
1.7 m (5.6 ft)
0.5 m (1.6 ft)
TN
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0 100 200 300 400 500
A
B
C
OHH
eigh
t
Average Cumulative Trap Captures (+ SE)
Xylosandrusgermanus
F = 544.64, df = 2, 14, P < 0.0001
0 5 10 15 20 25
A
B
BTN
Hei
ght (
m)
Average Cumulative Trap Captures (+ SE)
F = 21.57, df = 2, 14, P = 0.0001
0 10 20 30 40 50 60 70
A
B
BVA
Hei
ght (
m)
Average Cumulative Trap Captures (+ SE)
F = 50.48, df = 2, 46, P < 0.0001
2.0 mmUSDA-ARS, Wooster
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0 10 20 30 40
A
B
AB
Hei
ght
0 20 40 60 80
A
B
A
Hei
ght
VA (2006)
VA (2007)
0 2 4 6 8 10 12 14
A
B
C
Hei
ght
TN (2006)
0 40 80 120 160
A
B
A
Hei
ght
TN (2007)
Average Cumulative Trap Captures (+ SE)Average Cumulative Trap Captures (+ SE)
Xylosandruscrassiusculus
F = 34.47, df = 2, 14, P < 0.0001
F = 21.36, df = 2, 14, P = 0.0001
F = 8.61, df = 2, 14, P = 0.004
F = 3.93, df = 2, 14, P = 0.044
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Ohio Virginia Tennessee
Tota
l
0
1000
2000
3000
4000
Ohio Virginia Tennessee0
1000
2000
3000
4000
Total Captures in Three States During 2007
2.0 mmUSDA-ARS, Wooster
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ResearchInducing Tree Attacks
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Injecting Trees With Ethanol
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After Ethanol Injection
Attacks Induced on Previously Healthy Trees (Trunk Size ~ 4 Inches)
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Attacks on Adjacent Tree Shoots Attacks High in Tree
After Ethanol Injection
Injection Point
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After Ethanol Injection
Beetle Attempting to Enter TreeCheckered Beetle Attacking
Ambrosia Beetle Boring Into the Tree
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Ethanol-Baited Trap
Versus
Trap with Ethanol-Injected Bolt
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ResearchInsecticide Studies
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Insecticide / Biopesticide Sprays
Injecting Trees With Ethanol Using an Arbojet
Ambrosia Beetle Attacks Triggered
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Insecticide Test – April 24 to May 22, 2009
I. Trees Treated:A. Systemic Soil Drenches Rate / 100 gallons
- Acelepryn 32 floz- Safari 24 oz
B. Systemic Trunk Sprays- Safari 8 oz- Safari + PentraBark (wetting agent) 8 oz
C. Contact Pyrethroid Trunk Sprays- Onyx Pro Insecticide 32 floz- Perm-Up 3.2 EC 160 floz - Scimitar CS 5 floz
II. Trees Injected With Ethanol(75 ml of 50% ETOH)
- 34 days after systemic drenches- 1 day after trunk sprays
III. Trees Monitored for New Attacks (~ 1 month)
IV. Attacked Trees Then Held in Lab to Rear Beetles
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Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Acelepryn Drench
Total hits = 75
Apr24 Apr27 Apr30 May5 May11 May15 May22
0
3
6
9
12Safari Drench
*Total hits = 94
Mea
n N
ew A
mbr
osia
B
eetle
Atta
cks
(+ S
EM
)
Systemic Soil Drenches
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Control (Ethanol Injected)
Total hits = 70
Indicates significant difference from control (P < 0.05)*
(chlorantraniliprole)
(dinotefuran)
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Mea
n N
ew A
mbr
osia
B
eetle
Atta
cks
(+ S
EM
)
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Control (Ethanol Injected)
Total hits = 70
Systemic Trunk Sprays
Indicates significant difference from control (P < 0.05)*
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Safari Trunk Spray + Pentra Bark
Total hits = 49(dinotefuran)
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Safari Trunk Spray
*Total hits = 59
(dinotefuran)
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Mea
n N
ew A
mbr
osia
B
eetle
Atta
cks
(+ S
EM
)
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Control (Ethanol Injected)
Total hits = 70
Contact PyrethroidTrunk Sprays
Indicates significant difference from control (P < 0.05)*
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Perm Up Trunk Spray
* *
Total hits = 5(permethrin)
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Onyx Trunk Spray
*
Total hits = 42(bifenthrin)
Apr24 Apr27 Apr30 May5 May11 May15 May220
3
6
9
12Scimitar Trunk Spray
Total hits = 59(lambda-cyhalothrin)
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Biopesticide / Insecticide Test – June 24 to July 16, 2009
I. Trees Treated:A. Contact Pyrethroid Trunk Sprays (160 ml / 100 gal)
- Perm-Up 3.2 EC - Perm-Up 3.2 EC + PentraBark
B. Biopesticides (421 ml / gallon ~ 10% )- Cinnacure (30% cinnamaldehyde)- Cinnacure + PentraBark- EcoTrol (10% rosemary oil; 2% peppermint oil)
II. Trees Injected With Ethanol(75 ml of 10% ETOH)
- 1 day after trunk sprays
III. Trees Monitored for New Attacks (~ 1 month)
IV. Attacked Trees Then Held in Lab to Rear Beetles
Most antennallyactive on X. germanus
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Mea
n N
ew A
mbr
osia
Bee
tle A
ttack
s (+
SE
M)
Biopesticide Trunk Sprays
Indicates significant difference from control (P < 0.05)*
Cinnacure
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Total hits = 11
EcoTrol
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Total hits = 29
Cinnacure + PentraBark
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Total hits = 14
Control (No Injection)
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Control (Ethanol Injected)
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul16
0
2
4
6
Total hits = 34
Total hits = 13
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Pyrethroid Trunk Sprays
Control (No Injection)
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Control (Ethanol Injected)
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul16
0
2
4
6
Total hits = 34
Total hits = 13
Permethrin
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Total hits = 9
Permethrin + PentraBark
Jun24 Jun25 Jun26 Jun29 Jun30 Jul1 Jul2 Jul9 Jul160
2
4
6
Total hits = 18
Mea
n N
ew A
mbr
osia
Bee
tle A
ttack
s (+
SE
M)
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Biopesticide ambrosia beetle test - 2009Total Average Attacks
Permeth
rin
Cinn. +
Pen
tra
Cinnac
ureW
ater
Permeth
rin +
Pentra
Ethano
l Injec
ted
Ecotro
l
Mea
n +
SE
M
0
2
4
6
8
10
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ResearchPlant Stress Studies
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Ambrosia Beetle Tree Stress Test
Stress Treatments:1) Planting to deep2) Root drowning3) Delayed dormancy break4) Defoliation5) Drought stress
Data Collected:1) Number of new galleries
(3 times / week)2) Sticky trap catches3) Species reared from trees
Drought stress treatment
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Average Attacks + Standard Error
0 4 8 12 16 20
Delay May
Drought
Delay April
Defoliation
Plant Depth
Root Drowning
Control
Average Attacks + Standard Error
0 4 8 12 16 20
Delay May
Plant Depth
Drought
Delay April
Root Drowning
Defoliation
Control
Redbud Tulip Poplar
Stress Treatments – Average Total Attacks (April 24 to May 15, 2009)
No significant differences between treatments (P < 0.05)
Thysanoes fimbricornis
Asian or Granulated Ambrosia Beetle
Average Attacks (+ SEM) Average Attacks (+ SEM)
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Average Sticky Trap Captures (+ SEM)
0 20 40 60 80
Defoliation
Delay April
Plant Depth
Delay May
Root Drowning
Drought
Control
Average Attacks (+ SEM)
0 4 8 12 16 20
Delay May
Drought
Delay April
Defoliation
Plant Depth
Root Drowning
Control
Stress Treatments – Redbud Trees (Apr. 24 to May 15, 2009)
No significant differences between treatments (P < 0.05)
Tree Attacks(Thysanoes fimbricornis)
Sticky Trap Captures(all Scolytids)
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ManagementPlan
Preventing Damage
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Factors Increasing Attack Risk
1) Plants still dormant, but beetles being trapped
7) Planting near water sites (high humidity)
6) Time of year (March to May)
5) Fertilization terminated after July
4) Last pyrethroid spray over 4 weeks ago
3) Stressed crop (New transplant, drought, disease, freeze, poor drainage, trunk injury, etc.)
2) Susceptible species (Cherry, chestnut, dogwood, golden rain tree, lilac, maple, hydrangea, redbud,weeping mulberry, yellowwood, or others with past problems)
Ethanol release
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Use Traps for Early Detection of Activity
Look for Key Indicator Species
2.0 mmUSDA-ARS, Wooster
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- Thorough spray coverage
- Use effective insecticides:
Permethrin: Perm-Up 3.2 ECBiopesticides ???? (Future)
Insecticide Management
Management too lateif toothpick strands are present
Not labeled for ambrosia beetles
in walnutLabel Site: http://www.cdms.net/manuf/default.asp
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Some Conclusions- 3 species problematic in Tennessee nurseries (Xcrass, Xgerm, Xsax)
- Ethanol-baited traps catch large variety of ambrosia beetles- Different species have different activity periods- Within species peak captures varies by year (value of traps)- Trapping Xcrass, Xgerm, and Xsax coincides with tree attacks- Bottle trap was cheap and effective- Low height settings are best
- Ethanol injections induce Xylosandrus attacks - Value:- Trap trees ????- Timing insecticide sprays- Facilitate insecticide studies
- Attacks were induced in summer with ethanol
- Most induced attacks occurred during first week and then declined:- Change in host suitability ?????- Drop in ethanol release ????
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Some Conclusions
- Need more work to understand relationship between:- Types of plant damage and associated stress signals- Ambrosia beetle host selection factors- Host quality factors for the ambrosia fungus
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Acknowledgments
USDA-ARS, Wooster, OHJames Moyseenko Alane RobinsonBetsy Anderson Abby HartGerald Hammel Leona Horst
Multiple Commercial Nurseries
FundingUSDA-ARS Floriculture and Nursery Research InitiativeUSDA-ARS Specialty Crop Block GrantHorticultural Research Institute and American Nursery & Landscape Association USDA-APHIS Middle Tennessee Nursery Association
Virginia TechCarmella Whitaker
Tennessee State UniversityNadeer YoussefJoshua BashamJoshua MedleySamuel Patton