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Advisor : Cheng-Hsin Chuang

Advisee :Wan-Ting Su

Department of Mechanical Engineering & Institute of Nanotechnology,

Southern Taiwan University, Tainan, TAIWANDate 2012/04/06﹕

Paper Survey

A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce

Innovate Food Science and Emerging Technologies 12 (2011) 617-622

Sungkyun Kim ,Lin Lu, Jae-Hyun Chung ,Kyonghoon Lee ,Yong Li ,Soojin Jun

Outline

• Paper Survey Sensor Design Results and discussion

• Experimental Result

• Future work

Sensor Design

Fig .1.(a) Microwire sensing unit(b) Power supply and measurement equipments

Results and Discussion

DEP force and electroosmotic flow Capillary

Fig2.Regression curve for enumeration of E.coli K-12cells

Fig3.Stepwise produres for detection of E.coli K-12

Simulation Result

Fig 4. Sequential simulation of electric field strength

Experiment Results

Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire.(a)Without and (b) with AC field ﹒

Experiment Results

Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 106 CFU/ml

Fig .7. Fluorescence intensity of E.coli K-12 inPBS buffer when applied with 20VPP and 3MHz

Experiment Results

Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution:(a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations(b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface

Outline

• Paper Survey Sensor Design Results and discussion

• Experimental Result

• Future work

Experimental MethodExperimental

Experimental Results

Flow Rate ： 0.1 ml/hr

Al2O3 Concentration ： 2 wt%

BSA Concentration ： 1 μM

Applied Frequency ： 10 kHz

Apply Voltage ： 10 Vpp

Applied Time ： 0 min

Applied Time ： 2 min

Applied Time ： 16 min

Applied Time ： 30 min

Outline

• Paper Survey Sensor Design Results and discussion

• Experimental Result

• Future work

Future work

• Paper Survey

• Experiment by Different Parameter

• Experimental Sample Using Bladder Cancer Cell

• SEM Training

Thank you for your attention!!