Advisor: Wei-Shou Hu

Speaker: Justice Akhuamhenkhun Ace Lai

PRODUCTION OF 1,3-PROPANEDIOL THROUGH METABOLIC ENGINEERING

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Properties of 1,3-PD

• Molecular weight 76.09

• Viscous and colorless liquid

• Miscible with water

• Density = 1.0597 g/ cm

• MP = -28; BP, between 210 to 212 (celsius degree)

• Unsaturated compound with hydroxyl functional group

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Applications

• copolyesters, polymers, textile fibres, and carpets

• organic solvent ingredient of wood paints and anti-freeze

• chief ingredient manufacturing adhesives, laminates, terracota- as protective reagent

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Why we use bioengineering

• Cheaper in cost

• Easier in designing pathway

• Greener to environment

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Metabolic pathway

Metabolic engineering I

• nature of synthesizing of 1,3PD

• tpi gene

• dhaT and dhaB, gene encoding glycerol dehydratase and PDOR

• DAR1, GPP2 encoding 3-

phosphate dehydrogenase and glycerol 3-phosphate phosphatase

Metabolic engineering II

• Redesigning of yqhD

• Use of NADPH instead NADH

• knock down of tpi to manipulate pathway more efficiently

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Calculation analysis

• Yield, 1,3-propanediol at a rate of 3.5 g/L/h, a titer of 135 g/L, and a weight yield of 51% in D-glucosefed-batch 10 L fermentations.

9Conclusion• DAR1 and GPP2 cloned from

Saccharamyces cerevisiae encoding 3-phosphate dehydrogenase

• dhaB, dhaT gene from Klebsiella pneumoniae encoding PDOR and glycerol dehydratase

• yqhD from E. coli can produce PDOR as well

• tpi manipulated via knockdown to resolve carbon flux

• PEP and ATP dependent glucose pathway modified

• In all, contribute to higher yield synthesis