aerobic gram-positive bacilli part ii genital culture unit
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Please click audio icon to hear Carol’s narration. Aerobic Gram-Positive Bacilli Part II Genital Culture Unit. Division of Medical Technology Carol Larson MSEd, MT(ASCP). Click icon for audio. Differentiation of Major GPR Genera. Gram stain Morphology Arrangement Formation of spores - PowerPoint PPT PresentationTRANSCRIPT
Aerobic Gram-Positive BacilliPart II
Genital Culture Unit
Division of Medical Technology
Carol Larson MSEd, MT(ASCP)
Please click audio iconto hear Carol’s narration
• Gram stain– Morphology– Arrangement
• Formation of spores
• Catalase reaction
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Differentiation ofMajor GPR Genera
• Spore-forming GPR, catalase positive– Bacillus species– Bacillus anthracis– Bacillus cereus
• Nonspore-forming GPR, catalase positive– Listeria monocytogenes– Corynebacterium species (diphtheroids)– Corynebacterium diphtheriae– Corynebacterium jekeium
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GPR Discussed in Student Lab
• Nonspore-forming GPR, catalase negative– Gardnerella species– Erysipelothrix species– Lactobacillus species
• Branching Nocardioform GPR– Nocardia species– Streptomyces species
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GPR Discussed in This Lecture
• Pleomorphic gram-variable bacilli
• Non-sporulating
• Specimen gram stain:– Clue cells
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Gardnerella vaginalis
Gram Stain
GPR, pleomorphic, Non-spore forming, Catalase negative
• Does not grow on sheep blood agar
• Human Blood Agar (V-agar, HBT-agar)– Small colonies with diffuse zone of
beta-hemolysis
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Gardnerella vaginalis
Colony Morphology
GPR, pleomorphic, Non-spore forming, Catalase negative
• Catalase negative
• Oxidase negative
• Sodium hippurate usually positive
• SPS sensitive
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Gardnerella vaginalis
Identification
GPR, pleomorphic, Non-spore forming, Catalase negative
• Normal vaginal flora
• Bacterial vaginosis– Polymicrobic infection with Mobiluncus and
Bacteroides– Diagnosis
• Homogeneous, gray discharge• Clue cells• Amine or fishy odor when add 10% KOH• Culture not necessary for diagnosis
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Gardnerella vaginalis
Clinical Significance
GPR, pleomorphic, Non-spore forming, Catalase negative
What are the key identification characteristics to determine if Gardnerella vaginalis is the cause of bacterial vaginosis?
Examine vaginal specimen for presence of a homogeneous, gray discharge, clue cells on the Gram stain, and an amine or fishy odor when adding 10% KOH. A culture is not recommended, but if done, G. vaginalis grows on V-agar/HBT-agar and it beta hemolytic.
• Two types– Long slender GPR in chains– Short GPCB
• Non-sporulating
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Lactobacillus species
Gram Stain
GPR, pleomorphic, Non-spore forming, Catalase negative
• Grows on BAP– Multiple colony morphologies– Nonhemolytic or alpha hemolytic
• Microaerophilic
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Lactobacillus species
Colony Morphology
GPR, pleomorphic, Non-spore forming, Catalase negative
• Catalase negative
• Sucrose “F”
• Vancomycin “R”
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Lactobacillus species
Identification
GPR, pleomorphic, Non-spore forming, Catalase negative
Lactobacillus species
Clinical Significance• Normal flora
– Mouth– GI tract– Female vaginal tract
• Rarely pathogenic– Endocarditis– Meningitis
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GPR, pleomorphic, Non-spore forming, Catalase negative
What is the clinical significance of Lactobacillus species?
It is considered normal flora of the mouth, gastrointestinal tract, and female genital tract
• Nonspore-forming GPR, catalase negative– Gardnerella species– Erysipelothrix species– Lactobacillus species
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Review
• Nonspore-forming GPR, catalase negative– Gardnerella species– Erysipelothrix species– Lactobacillus species
• Branching Nocardioform GPR– Nocardia species– Streptomyces species
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Aerobic GPR
Summary
Who am I?
HBT media shows beta-hemolysis
GramStain
Gardnerella vaginalis
Causes Bacterial Vaginitis