aflatoxin contamination of red and black beans in costa rica · the mycotoxin laboratory at cigras...

• Aspergillus flavus had the ability to produce high aflatoxin levels in beans under the conditions tested in this experiment. Aflatoxin contamination has not been considered to be a problem in beans, however, these his results suggest that beans should be included in local monitoring programs. • There was no evidence of a relationship between bean seed coat color and aflatoxin contamination in the varieties examined (Guaymi, Matambú, Cabecar, Tayni and Surú). Future research should consider the examination of more bean varieties. • Based on these results, further research is necessary to determine how and when beans can become contaminated with aflatoxins in order to determine if bean type can be considered an important risk factor. Results showed that the A. flavus isolate used in this experiment could produce aflatoxins in concentrations greater than 150 μg kg -1 (Table 1), however, the ability of A. flavus to produce toxins was independent of both the bean variety and the presence or absence of the seed coat. The Mycotoxin Laboratory at CIGRAS collaborates with the Costa Rican State Phytosanitary Service (SFE) to monitor aflatoxin levels in imported agricultural commodities and with industry partners in order to assure quality control. Based on results for aflatoxin analyses conducted from 2003 to 2015, it was observed that red beans (Phaseolus vulgaris L.) had higher aflatoxin levels than black beans. Beans are a staple food in Costa Rica. Nonetheless, there is scarce information about aflatoxin contamination in beans, as well as the role of seed coat color and how it relates with mycotoxin contamination. Introduction Aflatoxin Contamination of Red and Black Beans in Costa Rica The objective of this study was to determine the level of aflatoxin contamination of two black bean varieties, two red bean varieties, and a white bean variety inoculated with Aspergillus flavus, in order to better understand the role of bean type in the risk of mycotoxin contamination. Objective Materials and Methods The experiment was conducted in the Mycotoxin Laboratory at CIGRAS, University of Costa Rica. Treatments consisted of 5 bean varieties: Guaymi and Matambú (black beans), Cabécar and Tayni (red beans), and Surú, a white bean variety. All varieties were subjected to seed coat removal (e.g., with and without the seed coat). Twenty-five grams of seeds of each treatment were placed in 125 ml Erlenmeyer flasks, autoclaved, and inoculated with 2 ml of a 1x10 7 spore solution of a toxigenic Aspergillus flavus isolate, which was previously isolated from bean seeds. Flasks were placed in an incubator at 28 o C, with a 12-h photoperiod for 22 days. Three flasks per treatment were used to make a composite sample to determine aflatoxin levels. Total aflatoxin contamination was measured using the AOAC method 991.31 (A- G). The experiment was replicated two times. Results Seed Coat Color Bean Variety Total aflatoxins (μg kg -1 ) With seed coat Without seed coat Black Guaymi 120.5 ± 24.1 115 ± 23 Matambú 39 ± 7.8 104 ± 20.8 Red Cabécar 90.5 ± 18.1 28.5 ± 5.7 Tayni 144.8 ± 29 57 ± 11.4 White Surú 107 ± 21.4 152 ± 30.4 Conclusions Table 1. Total aflatoxin (B 1 +B 2 +G 1 +G 2 ) concentration in different seed color bean varieties inoculated with Aspergillus flavus.

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Page 1: Aflatoxin Contamination of Red and Black Beans in Costa Rica · The Mycotoxin Laboratory at CIGRAS collaborates with the Costa Rican State Phytosanitary Service (SFE) to monitor aflatoxin

• There was no evidence of a relationship between bean seed coatcolor and aflatoxin contamination in the varieties examined(Guaymi, Matambú, Cabecar, Tayni and Surú). Future researchshould consider the examination of more bean varieties.

• Based on these results, further research is necessary to determinehow and when beans can become contaminated with aflatoxins inorder to determine if bean type can be considered an importantrisk factor.

Results showed that the A. flavus isolate used in thisexperiment could produce aflatoxins in concentrations greater than150 μg kg-1 (Table 1), however, the ability of A. flavus to producetoxins was independent of both the bean variety and the presenceor absence of the seed coat.

The Mycotoxin Laboratory at CIGRAS collaborates with the CostaRican State Phytosanitary Service (SFE) to monitor aflatoxin levelsin imported agricultural commodities and with industry partners inorder to assure quality control. Based on results for aflatoxinanalyses conducted from 2003 to 2015, it was observed that redbeans (Phaseolus vulgaris L.) had higher aflatoxin levels thanblack beans. Beans are a staple food in Costa Rica. Nonetheless,there is scarce information about aflatoxin contamination in beans,as well as the role of seed coat color and how it relates withmycotoxin contamination.

Introduction

Aflatoxin Contamination of Red and Black Beans in Costa Rica

The objective of this study was to determine the level of aflatoxincontamination of two black bean varieties, two red bean varieties,and a white bean variety inoculated with Aspergillus flavus, in orderto better understand the role of bean type in the risk of mycotoxincontamination.

Objective

Materials and Methods

The experiment was conducted in the Mycotoxin Laboratory atCIGRAS, University of Costa Rica. Treatments consisted of 5 beanvarieties: Guaymi and Matambú (black beans), Cabécar and Tayni(red beans), and Surú, a white bean variety. All varieties weresubjected to seed coat removal (e.g., with and without the seedcoat). Twenty-five grams of seeds of each treatment were placed in125 ml Erlenmeyer flasks, autoclaved, and inoculated with 2 ml of a1x107 spore solution of a toxigenic Aspergillus flavus isolate, which

was previously isolated from bean seeds. Flasks were placed inan incubator at 28oC, with a 12-h photoperiod for 22days. Three flasks per treatment were used to make acomposite sample to determine aflatoxin levels. Total aflatoxincontamination was measured using the AOAC method 991.31 (A-G). The experiment was replicated two times.

Results

Seed Coat

ColorBean Variety Total aflatoxins (μg kg-1)

With seed coat Without seed coat

Black Guaymi 120.5 ± 24.1 115 ± 23

Matambú 39 ± 7.8 104 ± 20.8

Red Cabécar 90.5 ± 18.1 28.5 ± 5.7

Tayni 144.8 ± 29 57 ± 11.4

White Surú 107 ± 21.4 152 ± 30.4

Conclusions

Table 1. Total aflatoxin (B1+B2+G1+G2) concentration in different seedcolor bean varieties inoculated with Aspergillus flavus.

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