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  • Agenda 1-22-13 Bell work #30 Write the complementary DNA strand of the strand below. TTAAGCGCTAATCGTACT Notes: Translation and Transcription Practice
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Agenda 1-22-13 Bell work #30 Write the complementary DNA strand of the strand below. TTAAGCGCTAATCGTACT Notes: Translation and Transcription Practice : DNA, mRNA, tRNA ( it may be homework) Closure: How does DNA differ from RNA?

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  • Agenda 1-22-13Bell work #30Write the complementary DNA strand of the strand below.TTAAGCGCTAATCGTACTNotes: Translation and Transcription Practice : DNA, mRNA, tRNA ( it may be homework) Closure: How does DNA differ from RNA?

  • Transcription and TranslationChapter 12p. 287-312

  • Protein StructureMade up of amino acidsPolypeptide- string of amino acids20 amino acids are arranged in different orders to make a variety of proteinsAssembled on a ribosome

  • Questions to be answered todayHow do we get from the bases found in DNA to amino acids? How do we get from a bunch of amino acids to proteins?

  • ReplicationDNA double helix unwindsDNA now single-strandedNew DNA strand forms using complementary base pairing (A-T, C-G)Used to prepare DNA for cell divisionWhole genome copied/replicated

  • Transcription and Translation: An Overview (aka the Central Dogma)TranscriptionTranslation

  • RNA vs. DNADNADouble strandedDeoxyribose sugar Bases: C,G A,T

    RNASingle strandedRibose sugarBases: C,G,A,UBoth contain a sugar, phosphate, and base.

  • TranscriptionRNA forms base pairs with DNAC-GA-UPrimary transcript- length of RNA that results from the process of transcription

  • TRANSCRIPTIONACGATACCCTGACGAGCGTTAGCTATCGUGCUAUGGGACU

  • Major players in transcriptionmRNA- type of RNA that encodes information for the synthesis of proteins and carries it to a ribosome from the nucleus

  • Major players in transcriptionRNA polymerase- complex of enzymes with 2 functions:Unwind DNA sequence Produce primary transcript by stringing together the chain of RNA nucleotides

  • mRNA ProcessingPrimary transcript is not mature mRNADNA sequence has coding regions (exons) and non-coding regions (introns) Introns must be removed before primary transcript is mRNA and can leave nucleus

  • Transcription is donewhat now?Now we have mature mRNA transcribed from the cells DNA. It is leaving the nucleus through a nuclear pore. Once in the cytoplasm, it finds a ribosome so that translation can begin.

    We know how mRNA is made, but how do we read the code?

  • TranslationSecond stage of protein productionmRNA is on a ribosome

  • Ribosomes2 subunits, separate in cytoplasm until they join to begin translationLargeSmallContain 3 binding sitesEPA

  • TranslationSecond stage of protein productionmRNA is on a ribosometRNA brings amino acids to the ribosome

  • tRNATransfer RNABound to one amino acid on one endAnticodon on the other end complements mRNA codon

  • tRNA FunctionAmino acids must be in the correct order for the protein to function correctlytRNA lines up amino acids using mRNA code

  • Reading the DNA codeEvery 3 DNA bases pairs with 3 mRNA basesEvery group of 3 mRNA bases encodes a single amino acidCodon- coding triplet of mRNA bases

  • How many bases code for each amino acid?1 base = 1 amino acid41 =

    2 bases = 1 amino acid42 =

    3 bases = 1 amino acid43 =

  • The Genetic Code

  • ACGATACCCTGACGAGCGTTAGCTATCGUGCUAUGGGACUG

  • Which codons code for which amino acids? Genetic code- inventory of linkages between nucleotide triplets and the amino acids they code forA gene is a segment of RNA that brings about transcription of a segment of RNA

  • Transcription vs. Translation ReviewTranscriptionProcess by which genetic information encoded in DNA is copied onto messenger RNAOccurs in the nucleusDNA mRNATranslationProcess by which information encoded in mRNA is used to assemble a protein at a ribosomeOccurs on a RibosomemRNA protein

  • 1-23-13 Wednesday Why knock on the door of opportunity when you have the key to open it? Bell Work #31 ( I will collect yesterdays assignment) Review over how to use the codon chart/questions from yesterdays work.Independent Practice: Using what You know: DNA and Protein Synthesis ( I will do some and then you will do the rest by yourself!) Quick write: Explain how you synthesis ( make) proteins from a strand of DNA?

    Objective: demonstrate an understanding of DNA replication, RNA transcription and translation. Reminders: Study Daily ( especially content related vocab). Quiz this Friday!!! ( replication, transcription, and translation) Lab: Next Monday and Tuesday ( bring .50 by Friday. letter sent home Thursday to parents

  • 1-24-13 Thursday Why knock on the door of opportunity when you have the key to open it? Bell Work #32( I will collect yesterdays assignment if you took it home to finish) Review bell work The Codon well ( its just like the chart) Independent Practice: Transcription and Translation summary ( lets using the codon well) Closure: reminders Objective: demonstrate an understanding of DNA replication, RNA transcription and translation. Reminders: Study Daily ( especially content related vocab). Quiz this Friday!!! ( replication, transcription, and translation) Journals are going to be graded Friday! Have your bell work 30-32, and quick write ready!Lab: Next Monday and Tuesday ( bring .50 by Friday.

  • The Codon Wheel

  • Extrashttps://mail.chisd.net/owa/attachment.ashx?attach=1&id=RgAAAAAy8cCE7aZOSos7BsxUDG6wBwAMEE5srlznRp5qwja5UkCyAvJ5tEZzAAAzbTlPvYonRYPxLnVQdUvJAAAMOl5NAAAJ&attid0=BAAAAAAA&attcnt=1

    Video of the notes from Mrs. Tates board

    *


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