agenda who done it workshop continental breakfast welcome and introductions who done it lab overview...
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AGENDAWho Done It Workshop
•Continental Breakfast•Welcome and Introductions•Who Done It Lab•Overview of SCBC•Resources on MATSC
• Reserving Your Kit on LARS
•Feedback
Agenda
• Forensics Overview• Pipetting: video, steps in procedure, practice, and
assessment• Gel Electrophoresis Part 1: video, card sort, and
prepare agarose gels (follow the SOP)• Practice pipetting and loading gel wells• Prepare your Samples• Gel Electrophoresis Part 2: load samples and run
gels• Analyze results and determine identity of criminal• Performance Assessment: Creating a Standard
Curve to identify an unknown
Who Done It?
Before you can put your Crime Scene Investigator hat and determine WHO committed the crime, you must first lean need to how to pipette and load dyes into a gel.
Part I - An Introduction to Pipetting
http://www.teachersdomain.org/resource/biot11.sci.life.gen.usingmicro/
When using a Pipette raise the tube to eye level and remember:
• Number in red represents the decimal place for the P-20
• Use the knob on the handle to change the volume
• Always use the pipette with a tip• Feel the pressure difference between first
stop and second stop• First stop aspirates (draws up the solution)• Second stop dispenses (pushes out) the
solution
Setting the Volume on a Micropipette: Think (Assessment)
1) The smallest amount of volume that I can measure with a P-20 pipette is…
2) When I use a P-20 pipette the maximum volume I can measure is…
Making an Agarose Gel
http://ca.pbslearningmedia.org/resource/biot11.sci.life.gen.agarosemake/making-an-agarose-gel//
Electrophoresis and Gel Analysis
http://ca.pbslearningmedia.org/resource/biot11.sci.life.gen.isolatedna/electrophoresis-and-gel-analysis/
Follow the SOP to Melt and Pour the Agarose Gel
CAUTION!!!! THE SOLUTION WILL BE EXTREMELY HOT AFTER MELTING- please use
provided mitt
1. Obtain one bottle of agarose solution as directed by your teacher.
2. LOOSEN the CAP and place in microwave for approximately 2 minutes or until agarose gel
solution is completely melted and clear.
3. Use mitt to remove melted solution from the microwave and place on the lab bench.
4. Remove the cap from the bottle and let the solution cool down to ~ 50 degrees Celsius
Place the gel tray in the chamber with the wells closest to the negative end
Pour buffer into the chamber so that it covers the gel completely
Place the hood on top of the chamber and press run button on the carriage
Let it run for 10-15 minutes