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Agilent 1100 Pump Module Maintenance

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Overall Routine Pump Maintenance

1. Remove the pump head

2. Replace the PTFE frit

3. Clean or replace the outlet ball valve

4. Replace the AIV cartridge (new model)

5. Disassemble the pump head

6. Exchange the piston seals

7. Clean and inspect pistons

8. Inspect the springs

9. Reassemble the pump head

10. Install all valves

11. Install the pump head

12. Flush with isopropanol (IPA)

13. Clean or replace solvent inlet filters

14. Clean the leak sensor

15. Make certain the waste tube is in place

16. Test (Pressure and Leak)

Procedure

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Pump Maintenance ConsumablesPurge valve frit (pack of 5) 01018-22707Cartridge AIV 5062-8562Gold seal AIV 5001-3708 Only old version Gold seal outlet valve 5001-3707Gold seal purge valve 5001-3707

Caps for purge valve/outlet valve 5062-2485Caps for AIV 5062-2485 Only for old versionSieves (pack of 10) 5063-6505 Only for binary pumpSapphire piston 5062-2441 old 5063-6586 NewSeals (RP) (pack of 2) 5062-8516 old 5063-6589 New Reversed PhaseSeals (NP) (pack of 2) 0905-1420 Normal Phase Wear Retainer 01018-22706 Not always necessary Seal Wash OptionSeals (pack of 1) 0905-1175Gasket (6 Stk.) 5062-2484Tools: G1311- 68705 Shipping Kit delivered with instrumentTweezers ------------wrench 14mm flat 8710-1924Hexagonal key ¼ - 5/16 inch 8710-0510Hexagonal key set 8710-0641Insert tool (Seal Wash Option) 01018-23702Lubricant 79846 - 65501

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Pump Head – Main Components

Plunger housing

Pump head

Outlet ball valve

Active inlet valve

Purge valve

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5

PistonsActive inlet valve

Seal

Support rings

Wear retainerPurge valveBall valve

Lock screw

Standard Pump Head – Exploded View

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Seal support assembly

Gasket

Seal

Optional for all Agilent 1100 pumps

Pump Head with Seal Wash Option

Gasket

Seals

Seal support assembly

Seal keeperTube

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Purge Valve – Exchanging the PTFE Frit

PTFE- frit

Gold Seal

Plastic cap

1. Unscrew the valve using a 14 mm wrench

2. Remove the plastic cap and the gold seal

3. Take out the frit (tweezers)

4. Install a new frit

5. Replace the gold seal and the plastic cap

6. Install the valve

Note: Realign the waste tube in the correct orientation during installation.

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Outlet Valve – Cleaning or Exchanging the Outlet Ball Valve

Valve housing

Gold Seal

Plastic cap

Video from 1100 Maintenance CD-ROM

1. Remove the capillary from the valve

2. Remove the valve using a wrench

3. Clean the ball valve in the ultrasonic bath or replace the ball valve

a. Take off the plastic cap and gold seal

b. Replace the ball valve

c. Replace the gold seal and cap

4. Reinstall

Note: The outlet ball valve of the binary pump has an additional sieve (5063-6505)

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Active Inlet Valve (AIV) – Old/New

Active Inlet Valve (AIV)

Cartridge8

New design

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Old design

1. Remove the AIV using a 14 mm wrench

2. Change the cartridge (new design)

3. Change the gold seals when necessary

4. Reinstall the AIV

Note: Properly position the AIV cable when you reinstall the valve

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Removing the Pump Head

1. Remove all capillaries 2. Disconnect the AIV supply cable3. Remove both pump head hexagonal

screws 4. Remove the pump

screw 4mm

screw 4mm

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Disassembling the Pump Head

The two parts of the pump head are disconnected by releasing thelock screw.

Video out of the maintenance CD-ROM

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Changing the Pump Seals

Video from the maintenance CD-ROM

1. Remove the old seals.

2. Remove the wear retainers, if present.

3. Clean the pump chambers.

4. Insert new seals.

5. Reassemble the pump head.

6. Perform seal wear-in procedure for standard seals (5063-6589).

Wear retainer

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Changing and Inspecting the Sapphire Pistons

1. Disassemble the pump head assembly

2. Check the plunger surface and remove any deposits or layers with alcohol or tooth paste

3. Replace the pistons if scratched

4. Reassemble the pump head

5. Check to make certain there are not any fractures in the springs

6. Put in the pistons

7. Put lubricant on the back of the pistons (79846-65501)

Video from the maintenance CD-ROM

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Installing the Seal Wash Option

Support KeeperWash Support ring

Plunger housing

Pistons

Video from the maintenance CD-ROM

1. Insert the seal wash option

2. Insert the mylar gaskets

3. Install the support keepers

4. Assemble the pump head (Lock screw)

5. Install the pistons

Gasket

Seal

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Is My Pump Well?

•Pump Ripple stable

•Pressure Test passes

•Retention Time Precision acceptable

•Area Precision acceptable

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Agilent 1100 Diode Array Detector

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Main DAD Components

Electronic boardOptical unit

Lamps

Detector cell Leak sensor

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DAD Routine Procedures and Maintenance

• Clean the leak sensors

• Check the waste tube

• Exchange the lamp

•Clean the flow cell

• Wavelength calibration

• Intensity test

• Detector cell test

• Dark current and filter test

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Exchanging the Detector Flow Cell Window I

Step 1: Remove the capillaries

Inlet

Outlet

Step 2: Take out the cell (Compress the spring bearing)

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Exchanging the Lamps

Step 1: Remove the lamp by unscrewing both screws and unplugging it.

Step 2: Install the lamp into the housing (auto-aligning) and screw snuggly.

Deuterium lamp 2140-0590

Tungsten lamp G1103-6001

Screwdriver

Required parts and tools

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Intensity Test

Limits:190-220nm > 2000220-350nm > 5000350-500nm > 2000500-950nm > 4000

Possible sources of errors:

- Absorbing solvent- Contaminated flow cell- Contaminated optical components (achromat, window)

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Holmium Oxide TestIn the holmium oxide test three characteristic wavelength maxima are compared.The slit setting is fixed at 1 nm.

A deviation of one or more wavelengths leads to a "Failed“Test.

Limits:360.8 nm (359.8-361.8418.5 nm (417.5-419.5)536.4 nm (535.5-537.4)

Possible sources of errors:- absorption of solvent- incorrect wavelength calibration- contaminated flow cell- solarized optical components ( achromat, grating , mirrors)

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DAD Test Functions in the Software

DAD Self-Test •Filter Test•Slit Test•Dark Current Test•Wavelength Calibration Test•Intensity Test•Holmium Test•Spectral Flatness Test•ASTM Noise Test (optional)

Maintenance Summary

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Summary of Agilent 1100 Series Maintenance

• Clean or Replace the solvent inlet filter

• Flush the degasser

• Perform pump maintenance

• Perform autosampler maintenance

• Perform column switching valve maintenance (if installed)

• Perform detector maintenance

The time needed is approximately 4 hours for a complete system

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Routine Maintenance - Overview

Solvent inlet Pump Autosampler Detector

Clean or Exchange

Solvent inlet filter

Exchange

PTFE frit

Pump seals

Ball Valve

AIV Valve cartridge

Piston wash seals

Clean

Pistons

Support ring

Leak sensor

Check:

Piston springs

Drain tube

Exchange:

*Needle

*Needle seat

*Rotor seal

Clean

Leak Sensor

Check

Drain tube

Exchange

*Lamp

*Cell window

Cleaning:

Cell

Check:

Leak sensor

* ... if necessary

Tests

Column Compartment

Replacing the rotor seal

(Column valve)

Check:

Leak sensor

Drain tube

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Recommended Tests to Conclude Maintenance

Pump Autosampler Detector

Pressure test

Leak test

Pressure test (mainpass, bypass)

Injection of a standard

Detector specific tests

Column compartment

Temperature stability

Pressure test

(Column valve)

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Maintenance Frequency

•The frequency of maintenance is dependent uponyour usage and applications.

•In general, some perform overall maintenance onceper year.

•depending on the lab situation and the usage it mightbe necessary to do it every 6 months.

•Use Early Maintenance Feedback once you determinethe necessary frequency.