agilent new 6470 triple quadrupole lc/ms system€™s nothing... · introducing the 6470 triple...

Agilent New 6470 Triple Quadrupole LC/MS System

ROCK SOLID Performance For Confident Quantitation

Na Pi Parra, Ph.D

Product Manager for Triple Quadrupole LC/MS

6/23/2015

ASMS 2015 Breakfast Seminar

1

Overview of Topics

The New 6470 QQQ LC/MS System

• Technology innovations

• Quantitation performance

Key Applications

• Food safety

• Environmental

• Pharmaceutical

Summary

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Introducing the 6470 Triple Quadrupole LC/MS

• Rock Solid Performance

• Improved Sensitivity

• Lower IDL < 4.0 fg

• Smaller Footprint

• Upgradeable to 6495

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Higher Throughput

Higher Confidence

Higher Productivity

64606470

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6470 QQQ TechnologiesEnhanced Performance in a Smaller Space

Agilent Jet Stream Technology

• Thermal gradient focusing

• Efficient desolvation

• Creates an ion rich zone

• Up to 10x gains in sensitivity

• An Ion Detector with High Energy Conversion Dynode and Low Noise

• Improved ion detection3

• A Curved and Tapered Hexapole Collision Cell

• Effective ion collection2

• Enhanced Q1 Ion Optics

• Improved ion transmission1

Enhanced Q1 Ion Optics with Optimized PrefilterImproved transmission of precursor ions and additional system robustness

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1. Reduced Octopole length1

1. New prefilter2

1

2

• New optimized MS 1 prefilter geometry for improved precursor ion transmission

– Improved peak area response and peak area %RSD, more sensitive and precise

• New optical lens elements for reduced the probability of contamination

– More reliable and robust performance

Curved and Tapered Hexapole Collision Cell Effective collection and transmission of product ions and smaller footprint

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• Curved and Tapered Hexapole Assembly for efficient collection and transmission of

product ions

• Designed for consistent collision energies across all QQQ platforms

• A compact, smaller benchtop footprint

6460 QQQ LC/MS

- Linear Collision Cell

6470 QQQ LC/MS

- Curved Collision Cell

30% smaller

New Detector with High Energy Conversion DynodeMore efficient detection and quantitation of ions with low noise characteristics

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• Improved ion detection efficiency with High Energy Dynode (HED) voltage up to 20 kV

- Improved peak area response and peak area %RSD in positive & negative ion mode

- Improved sensitivity and precision for a wide mass range

• Low noise level at 20 kV

- Improved signal to noise

40 – 113% increase in

response to 20kV vs. 10kV

6470 QQQ – Rock Solid, Improved PerformanceRobust and Reliable, More Sensitive, More Compact, and Upgradeable

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Proven robustness and reliability in complex matrix

- Extensively tested in key applications

Improved sensitivity and lower LLOQs

– In S/N specifications and key applications

Improved precision and excellent accuracy

– In IDL specifications and key applications

Improved scan and MRM speed, and polarity switching

Proven 6 orders of linear dynamic range

Smaller (30%) footprint and upgradeable to the 6495

Per

form

ance

Robustness

Footprint

Unprecedented Robustness in Biological MatrixQuantitation of Drugs in Plasma using RapidFire 365 + 6470 QQQ

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• Stable peak area response over 10,000 injections of plasma samples and 5 days of

continuous operation

• Response %RSD = 3.7 – 8.6

Injection #1

Injection #10,000

Injection #1

Injection #10,000

Time (Sec)

Time (Sec)

Love et al.

WP #496

Improved Sensitivity and Precision – Lower IDLsReserpine (+) and Chloramphenicol (-), 6470 vs. 6460

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10 fg reserpine (+) on the 6470 10 fg chloramphenicol (-) on the 6470

IDL = 2.5 fg %RSD = 8.7

n = 10 injections

%RSD = 10.2

n = 10 injections

IDL = 2.9 fg

Models Reserpine (+) Chloramphenicol (-)

Measured at %RSD IDL Measured at %RSD IDL

6460 20 fg 9.6 5.4 fg 20 fg 11.9 6.7 fg

6470 10 fg 10.2 2.9 fg 10 fg 8.7 2.5 fg

6470 achieves 2-3x lower IDLs vs. the 64606470 IDLs are measured at a 2x lower level

Improved Sensitivity and Precision – Lower LLOQAcetaminophen LLOQ in Water, 6470 vs. 6460

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• Improved peak area (4x) and area %RSD (2x) were observed on the 6470 compared to the 6460

• Lower LLOQ (4x) was observed on the 6470 compared to the 6460

• Excellent precision (%RSD) and accuracy were achieved at the LLOQ level of 6470

LLOQ = 2 ng/L on the 6460

6470

Peak Area = 5863

S/N = 34.6

Area %RSD = 4.3

6460

Peak Area = 1490

S/N = 11.1

Area %RSD = 9.5

n = 8 injections

LLOQ = 0.5 ng/L on the 6470

Blank

6470

Peak Area = 1656

S/N = 10.6

Area %RSD = 8.3

%Accuracy = 104.8

6460

Peak Area = 423

S/N = 4.0

Area %RSD = 19.0

% Accuracy = 77.0

n = 8 injections

Excellent Precision at the Lowest Levels – Lower IDLAcetaminophen IDL measured at the LLOQ level, 6470 vs. 6460

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Models Amount measured - at LLOQ Replicates %RSD IDL

6460 2 ng/L n = 8 9.5 0.59 ng/L

6470 0.5 ng/L n = 8 8.3 0.12 ng/L

• Lower LLOQ (4x) and IDL (5x) were achieved on

the 6470 compared to the 6460

Inj # Peak Area S/N

1 1435 22.2

2 1419 10.6

3 1373 11.0

4 1282 10.6

5 1343 7.6

6 1431 15.5

7 1656 15.2

8 1553 10.6

%RSD 8.3 Ave 12.8

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

Acetaminophen

6470 IDL = 0.1 ng/L

6460 IDL = 0.5 ng/L

Blank

Attogram Sensitivity (+)500 attograms of Clenbuterol on-column

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LLOQ of Clenbuterol on the 6470

500 attogram on-column

Blank

Area %RSD = 12.4

n = 3 injections

%Accuracy = 107.2

S/N = 5.9

Excellent precision (%RSD) and accuracy are observed at the LLOQ level of 6470

Attogram Sensitivity (-)800 attograms of Chloramphenicol on-column

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Area %RSD = 18.1

n = 3 injections

%Accuracy = 98.2

S/N = 18.0

Excellent precision (%RSD) and accuracy are observed at the LLOQ level of 6470

LLOQ of Chloramphenicol on the 6470

800 attogram on-column

Blank

5.7 Orders of Linear Dynamic Range Clenbuterol (+), 0.1 pg/mL – 50 ng/mL

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• Excellent linearity, R2 = 0.9995

• Superb assay accuracy and precision are achieved at all

levels including the LLOQ level of 0.1 pg/mL

Levels%RSD

(n = 3)

%

Accuracy

0.1 pg/mL 12.4 107.2

0.5 pg/mL 9.4 100.2

1 pg/mL 4.4 81.0

5 pg/mL 1.7 81.1

50 pg/mL 0.36 83.0

500 pg/mL 2.0 88.2

5 ng/mL 0.60 105.6

50 ng/mL 0.20 99.6

Applications for 6470 QQQ LC/MS

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Food Safety

Analysis of multi-residue pesticides in food matrices with higher degrees of sample dilution

Environmental

Determination of PPCPs in surface water using direct injection

Pharmaceutical – High-Capacity Multisampler UHPLC

High-throughput analysis of drugs & metabolites in plasma with reliable 24/7 operation

Multi-Residue Pesticide Analysis in Food

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• Multi-residue pesticide analysis in food products – most demanding food safety applications

• Improved sensitivity and precision of the 6470 allows accurate quantitation of pesticides

<Maximum Residue Limits (MRLs) imposed by EU, with higher degrees of sample dilution

• Sample dilution reduces matrix effects, improves method robustness, allows more efficient

ionization and enables the use of solvent calibration with better accuracy

Injection volume = 2 µL

1:20 dilution of > 250 pesticides spiked into black tea at 10 ng/mL (ppb)

Sartain et al.

WP #059

Sensitivity Improvements for High Relevance Pesticides1:20 Dilution of 10 ng/mL of Pesticides in Black Tea, Peak Area 6470 vs. 6460

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• Improved sensitivity (average peak area gain of 3.0x) are observed on the new 6470 vs. 6460

6470 6460

Oxamyl

2.8x

Methomyl

3.9x

Area Gain

Thiamethoxam

2.5x

6470 6460 Area Gain

Acetamiprid

3.4x

Thiacloprid

2.7x

Ethion

3.2x

Improved Precision and Lower IDLsIDLs of 30 Highly Relevant Pesticides, 6470 vs. 6460

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• Improved precision (peak area %RSD) are observed, particularly at the lowest levels

• The enhanced peak area response and improved precision (%RSD) leads to lower

LLOQs and IDLs compared to the 6460

• Lower IDLs (average improvement of 3.6x) was observed across all > 250 pesticides

Pesticide IDL (ppt) Fold-

Improvement

Pesticide IDL (ppt) Fold-

Improvement 6470 6460 6470 6460

Acephate 7.1 10.4 1.5x Fipronil 74.5 339.7 4.6x

Acetamiprid 1.8 32.9 18.0x Flufenoxuron 58.0 521.6 9.0x

Azinphos-methyl 165.1 278.9 1.7x Imazalil 30.1 203.2 6.7x

Buprofezin 6.2 36.0 5.8x Imidacloprid 57.6 191.1 3.3x

Carbendazim 3.7 13.5 3.6x Isocarbophos 14.6 129.5 8.9x

Chloroxuron 7.4 98.4 13.3x Metamitron 172.6 558.4 3.2x

Cycluron 8.4 71.1 8.4x Methidathion 71.9 340.1 4.7x

Desmedipham 11.2 32.4 2.9x Methomyl 4.1 21.5 5.2x

Diethofencarb 11.1 59.0 5.3x Monocrotophos 10.8 52.3 4.8x

Dimethoate 2.8 32.8 11.8x Oxamyl 0.9 5.8 6.2x

Dimethomorph 59.0 310.7 5.3x Pirimicarb 0.8 2.8 3.6x

Dimoxystrobin 6.3 23.6 3.8x Pyridaben 0.8 0.6 0.8x

Diniconazole 30.9 237.4 7.7x Tebuconazole 45.8 41.9 0.9x

Dioxacarb 103.7 139.1 1.3x Teflubenzuron 291.6 2012.1 6.9x

Diuron 22.6 37.4 1.7x Triazophos 10.1 65.9 6.6x

Average Improvement 3.6X

Femtogram Sensitivity for Pesticides in Black TeaLLOQs and IDLs / MDLs at Low-pg/mL level on the 6470

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• LLOQs 100 pg/mL are required to achieve the MRL with 1:20 dilution of black tea

• Excellent peak area precision (%RSD) and accuracy are observed at the LLOQs in black tea

Pesticides

Oxamyl (+)

Pirimicarb (+)

Bentazone (-)

Flubendiamide (-)

Black Tea

LLOQ

(pg/mL)%Accuracy

%RSD

(n = 5)

MDL

(pg/mL)

10 108.9 9.5 3.6

10 103.7 10.4 3.9

10 98.2 19.2 7.2

20 105.4 17.8 13.3

Neat Solvent

LLOQ

(pg/mL)%Accuracy

%RSD

(n = 5)

IDL

(pg/mL)

2 114.0 12.6 0.94

2 117.2 10.3 0.77

5 81.8 16.3 3.1

10 86.0 12.4 4.7

Bentazone 10 pg/mL

S/N = 17.6

Oxamyl 10 pg/mL

S/N = 10.4

Flubendiamide 20 pg/mL

S/N = 195Pirimicarb 10 pg/mL

S/N = 12.7

LLOQs in Black Tea

Improved Sensitivity and Precision of the 6470 Allows for Higher Degree of Sample Dilution

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• Majority of the pesticides achieved an LLOQ of 5% of the MRL level in food matrices

• Pesticides can be confidently quantified in food matrices with up to 1:20 dilution

• Sample dilution leads to reduced matrix effects and improved method robustness

• Sample dilution allows for more efficient ionization and significantly better recoveries

Histograms of LLOQs for Evaluated Pesticides in Solvent and Food Matrices

≤ 100 ppt 100-200 ppt 200-500 ppt 500 ppt - 1 ppb 1-10 ppb >10 ppb

171

3226

10 9 11

163

3826

9 13 10

101

3446

27 3121

Tomato Orange Black Tea

Improved Recoveries at Higher Degree of Dilution

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0

10

20

30

40

50

60

70

80

90

100

<30% 30-50% 50-70% 70-120% >120%

Num

ber o

f Pes

ticid

es

Recoveries

undiluted

1 to 2

1 to 5

1 to 10

1 to 20

PassesSANCO

Requirements

• 116 pesticides showed strong ion suppression in undiluted black tea

• Most of pesticides achieved acceptable recoveries of 70-120% and less signal

suppression with 1:20 dilution, which are in full compliance with SANCO requirements

• Neat solvent calibration curve can be used for pesticide quantitation with better accuracies


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Food Safety


Environmental




Determination of PPCPs in Environmental Water

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• PPCPs in surface waters may have an adverse impact on wildlife and human even at very low

concentrations (ng/L)

• The improved sensitivity and precision of the 6470 allows accurate quantitation of PPCPs at

sub-ng/L level using direct injection without the need of time-consuming SPE enrichment

• Direct injection improves analysis efficiency, assay accuracy, and lab productivity

28 PPCPs spiked in water at 10 ng/L (ppt)

Injection volume = 40 µL

24X IN POS Mode Erythromycin (+)

Acetaminophen (+) Fluridone (+)

Atenolol (+) Gabapentin (+)

Atrazine (+) Lamotrigine (+)

Bupropion (+) Metoprolol (+)

Caffeine (+) Propranolol (+)

Carbamazepine (+) Sucralose (+)

Clarithromycin (+) Sulfamethoxazole (+)

Cotinine (+) Trimethoprim (+)

DEET (+) Venlafaxine (+)

Dextrorphan (+) 4x in NEG Mode

Diazinon (+) 2,4-D (-)

Diltiazem (+) Gemfibrozil (-)

Diphenhydramine (+) Triclopyr (-)

Diuron (+) Triclosan (-)

Li et al.

TP #305

Improved Sensitivity for PPCPs in Positive Mode10 ng/L of PPCPs in Water, Peak Area 6470 vs. 6460

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Acetaminophen 2.6x

Atenolol 2.3x

Atrazine 3.3x

Bupropion 3.5x

Caffeine 2.4x

Carbamazepine 2.2x

Clarithromycin 4.5x

Cotinine 2.9x

DEET 2.6x

Dextrorphan 1.8x

Diazinon 2.6x

Diltiazem 2.1x

• Improved sensitivity (average peak area gain of 2.9x) are observed across 24 PPCPs in POS mode

6470

6460

Improved Sensitivity for PPCPs in Negative Mode10 ng/L of PPCPs in Water, Peak Area 6470 vs. 6460

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2, 4-D 4.1x Triclopyr 3.8xGemfibrozil 2.3x Triclosan 2.7x

• Improved sensitivity (average peak area gain of 3.2x) are observed across 4 PPCPs in NEG mode

10 ppt on 6460

10 ppt on 6470

2.9x in POS compounds

3.2x for NEG compounds

Average improvement = 3.0x

Overlaid MRM chromatograms: 6470 vs. 6460

6470

6460

Improved Precision – Comparison of Area %RSD5 ng/L of PPCPs in Water, 6470 vs. 6460

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• Improved precision (peak area %RSD) are observed, particularly at the lowest levels

• Average improvement of 2.3x was observed across 28 PPCPs analyzed

• The enhanced peak area response and improved precision (%RSD) leads to lower IDLs and

LLOQs compared to the 6460

Compounds%RSD (n=8) Improvement

Compounds%RSD (n=8)Improvement

Acetaminophen 0.6x Erythromycin 1.3x

Atenolol 1.6x Fluridone 6.0x

Atrazine 2.0x Gabapentin 1.7x

Bupropion 2.7x Lamotrigine 3.3x

Caffeine 3.6x Metoprolol 1.3x

Carbamazepine 4.9x Propranolol 1.8x

Clarithromycin 1.3x Sucralose 1.2x

Cotinine 3.0x Sulfamethoxazole 1.6x

DEET 2.2x Trimethoprim 2.1x

Dextrorphan 1.8x Venlafaxine 2.0x

Diazinon 2.0x 2,4-D 2.1x

Diltiazem 1.4x Gemfibrozil 3.6x

Diphenhydramine 4.0x Triclopyr 2.4x

Diuron 1.0x Triclosan 1.2x

Average Improvement 2.3x

Lower IDLs at Sub-ng/L Level IDLs of PPCPs in water, 6470 vs. 6460

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• Lower IDLs (average improvement of 3.6x) were observed across all 28 PPCPs (POS and NEG)

PPCPs6460 IDL

(ng/L)

6470 IDL

(ng/L)Improve

mentPPCPs

6460 IDL

(ng/L)

6470 IDL

(ng/L)Improve

ment

Acetaminophen 0.59 0.12 4.9x Erythromycin 0.96 0.14 6.8x

Atenolol 0.64 0.45 1.4x Fluridone 0.053 0.012 4.5x

Atrazine 0.44 0.28 1.6x Gabapentin 1.89 1.05 1.8x

Bupropion 0.17 0.044 3.9x Lamotrigine 2.15 0.94 2.3x

Caffeine 1.20 0.25 4.8x Metoprolol 1.02 0.29 3.5x

Carbamazepine 0.32 0.082 3.9x Propranolol 0.75 0.091 8.2x

Clarithromycin 3.58 1.14 3.1x Sucralose 19.5 3.56 5.5x

Cotinine 0.57 0.068 8.0x Sulfamethoxazole 0.87 0.41 2.1x

DEET 0.067 0.022 3.1x Trimethoprim 0.64 0.39 1.6x

Dextrorphan 1.01 0.15 6.7x Venlafaxine 0.22 0.076 2.8x

Diazinon 0.17 0.071 2.4x 2,4-D 34.4 8.69 4.0x

Diltiazem 0.13 0.030 4.2x Gemfibrozil 22.1 11.5 1.9x

Diphenhydramine 0.13 0.052 2.4x Triclopyr 38.0 16.3 2.3x

Diuron 0.56 0.28 2.0x Triclosan 14.6 5.32 2.7x

Average Improvement 3.6x

IDLs of 24 PPCPs in Positive Mode

IDLs of 4 PPCPs in Negative Mode

6470 IDL 6460 IDL

6470 IDL 6460 IDL

Lower LLOQs – Femtogram Sensitivity for PPCPsLLOQs of PPCPs in water, 6470 vs. 6460

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• Lower LLOQs (average improvement of 2.7x) were achieved across all 28 PPCPs (POS and NEG)

• LLOQs for most of the PPCPs are much lower than the ng/L (ppt) level regulation limit

• Excellent peak area precision (%RSD) and accuracy are observed at the LLOQs

PPCPs 6470 LLOQ

(ng/L)

6460 LLOQ

(ng/L) PPCPs

6470 LLOQ

(ng/L)

6460 LLOQ

(ng/L)

Acetaminophen 0.5 2 Erythromycin 1.0 2

Atenolol 1.0 2 Fluridone < 0.1 0.5

Atrazine 0.5 2 Gabapentin 5.0 10

Bupropion 0.2 1 Lamotrigine 2.0 10

Caffeine 1.0 2 Metoprolol 1.0 2

Carbamazepine 0.5 2 Propranolol 1.0 2

Clarithromycin 5.0 10 Sucralose 20.0 50

Cotinine 0.5 2 Sulfamethoxazole 1.0 2

DEET 0.2 0.5 Trimethoprim 1.0 2

Dextrorphan 1.0 2 Venlafaxine 0.5 1

Diazinon 0.5 1 2,4-D 20.0 50

Diltiazem 0.2 0.5 Gemfibrozil 20.0 50

Diphenhydramine 0.2 0.5 Triclopyr 50.0 100

Diuron 1.0 2 Triclosan 20.0 50

Improvement 2 – 5 X

%RSD = 3.8 (n=8)%Accuracy = 102.3S/N = 66

%RSD = 8.3 (n=8)%Accuracy = 104.8S/N = 22

%RSD = 7.3 (n=8)%Accuracy = 99.0S/N = 35.1

%RSD = 8.9 (n=8)%Accuracy = 107.9S/N = 33.4

Acetaminophen 0.5 ng/L Fluridone 0.1 ng/L

Bupropion 0.2 ng/L Triclosan 20 ng/L


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Food Safety


Environmental




Analysis of Drugs and Metabolites in Human Plasma6470 QQQ with High-Capacity Multisampler UHPLC

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• PK analysis of drugs and metabolites is challenged with the need to perform large-scale-analyses

with high sensitivity (pM) and sufficient dynamic range (pM nM) in biological matrices

• The highly sensitive 6470 enables fast and accurate quantitation of drugs and metabolites at

low-pM level over a wide linear dynamic range in human plasma

• 6470’s robustness together with the high-capacity multisampler allows for high-throughput drug

analysis with 24/7 unattended operation and extremely reliable results

-Hydroxymidazolam

Midazolam

Alprazolam

- Hydroxyalprazolam

-OH MidazolamMidazolam

Alprazolam -OH Alprazolam

Benzodiazepines and metabolites spiked in human plasma at 5 pg/mL

Yang et al.

TP #297

High Sensitivity for Benzodiazepines in Plasma LLOQs and MDLs at Low-pg/mL level on the 6470

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• Excellent peak area precision (%RSD) and accuracy are observed at the LLOQ level

-Hydroxymidazolam

LLOQ = 5 pg/mL

S/N = 13.6

Midazolam

LLOQ = 5 pg/mL

S/N = 35.2

-Hydroxyalprazolam

LLOQ = 5 pg/mL

S/N = 5.4

Alprazolam

LLOQ = 5 pg/mL

S/N = 9.2

CompoundLLOQ

(pg/mL)%Accuracy

%RSD

(n = 7)

MDL

(pg/mL)

-Hydroxymidazolam 5 100.7 6.9 1.1

Midazolam 5 114.6 5.3 0.84

-Hydroxyalprazolam 5 99.4 9.9 1.6

Alprazolam 5 96.1 7.5 1.2

Confident Quantitation over 5 Orders of Dynamic Range

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• Excellent linearity (R2 > 0.995), accuracy and precision are achieved at all levels including LLOQ

• Drugs and metabolites at different levels can be quantified simultaneously and in one experiment

Levels %RSD (n = 7) %Accuracy

5 pg/mL 5.32 114.6

25 pg/mL 2.76 96.6

50 pg/mL 2.53 102.8

250 pg/mL 1.22 98.6

500 pg/mL 0.73 98.7

2.5 ng/mL 0.51 97.6

5 ng/mL 0.34 99.1

25 ng/mL 0.56 96.5

50 ng/mL 0.79 96.4

250 ng/mL 0.75 97.3

500 ng/mL 0.47 101.9

Midazolam, 5 pg/mL – 500 ng/mL

5 Orders of Linear Dynamic Range

R2 = 0.9994

Concentration (ng/ml)0.005 0.01 0.05 0.1 0.5 1 5 10 50 100 500 1000

Rela

tive

Res p

ons e

s 2x10

5E-06

1E-05

5E-05

0.0001

0.0005

0.001

0.005

0.01

0.05

0.1

0.5

Levels %RSD (n = 7) %Accuracy

5 pg/mL 7.54 96.1

25 pg/mL 2.77 96.9

50 pg/mL 2.95 101.9

250 pg/mL 1.62 101.3

500 pg/mL 1.20 101.8

2.5 ng/mL 0.42 99.8

5 ng/mL 1.24 102.3

25 ng/mL 2.15 100.1

50 ng/mL 0.77 99.7

250 ng/mL 2.91 100.0

500 ng/mL 0.62 86.6

Alprazolam, 5 pg/mL – 500 ng/mL

5 Orders of Linear Dynamic Range

R2 = 0.9970

Concentration (ng/ml)0.005 0.01 0.05 0.1 0.5 1 5 10 50 100 500 1000

Rela

tive

Res p

ons e

s 2x10

5E-06

1E-05

5E-05

0.0001

0.0005

0.001

0.005

0.01

0.05

0.1

0.5

Proven System Robustness in Human Plasma

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• Stable peak area response was observed over 1,300 injections of plasma extract and 1 week

of continuous operation

• Average area ratio %RSD = 1.8 was observed with use of internal standard (ISTD)

• Average peak area %RSD = 6.6 was observed for benzodiazepines and metabolites

0

0.2

0.4

0.6

0.8

1

1.2

0 200 400 600 800 1000 1200 1400

Peak A

rea R

ati

o

Injection number

-Hydroxymidazolam

0

0.2

0.4

0.6

0.8

1

1.2

0 200 400 600 800 1000 1200 1400

Peak A

rea R

ati

o

Injection number

Midazolam

0

0.2

0.4

0.6

0.8

1

1.2

0 200 400 600 800 1000 1200 1400

Peak A

rea R

ati

o

Injection number

-Hydroxyalprazolam

0

0.2

0.4

0.6

0.8

1

1.2

0 200 400 600 800 1000 1200 1400P

eak A

rea R

ati

oInjection number

Alprazolam

Area Ratio %RSD=1.3

Peak area %RSD=5.7

Area Ratio %RSD=3.3

Peak area %RSD=7.0

Area Ratio %RSD=1.4

Peak area %RSD=7.2

Area Ratio %RSD=1.3

Peak area %RSD=6.3

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Summary: 6470 QQQ LC/MSRock Solid Performance for Confident Quantitation and Highest Lab Productivity

Additional Robustness

Improved Sensitivity

Improved Precision

Faster Speed

Smaller Footprint

Upgradeable to the 6495

Ion Optics

20KV Detector

Curved Collision Cell

Less Maintenance Cleaning

Reliable Consistent Results

over Longer Time (24/7)

Streamlined Analytical

Workflow

Reproducible

High Quality Data

Higher Throughput

at UHPLC Speed

More Instruments

In Your Laboratory

Protection of

Your Investment


6/23/2015


36

ASMS 2015, St Louis6470 Marketing Launch

Breakfast Seminar

Technical VIP

Technical Posters

Brochure

App Note

Hospitality Suite

Agilent Booth

6/23/2015


37

agilent new 6470 triple quadrupole lc/ms system€™s nothing... · introducing the 6470 triple...

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