MOLECULAR DIAGNOSTICS

AIT Austrian Institute of Technology GmbHCenter for Health amp Bioresources

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY

OWNERSHIP STRUCTURE

REPUBLIC OF AUSTRIA (through the Federal Ministry for Transport Innovation and Technology)

FEDERATION OF AUSTRIAN INDUSTRIES

5046

4954

EMPLOYEES

1300

77 m EUR Contract research revenues (incl Grants)45 m EUR bmvit funding21 m EUR Other operating income

incl Nuclear Engineering Seibersdorf

TOTAL REVENUES

143 m EUR

NuclearEngineering Seibersdorf

GmbH

SeibersdorfLabor GmbH

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY

AIT Austrian Institute of Technology

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

AIT Austrian Institute of Technology

Mobility Systems Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

FOCUS OF THE CENTERS

bull Sustainable Thermal Energy Systems

bull Electric Energy Systemsbull Sustainable Buildings and

Citiesbull Photovoltaic Systemsbull Environmental Resources

amp Technologies

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

Mobility Systems

Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

bull Bioresourcesbull Molecular Diagnosticsbull Biomedical Systemsbull Digital Health Information

Systems

bull Security amp Communication Technologies

bull Visual Surveillance and Insight

bull Smart Sensor Solutionsbull Dependable Systems

Engineeringbull Information Management

bull High-Performance Image Processing

bull Autonomous Systemsbull Complex Dynamical

Systems

bull Transportation Infrastructure Technologies

bull Dynamic Transportation Systems

bull Electric Drive Technologies

bull Light Metals Technologies Ranshofen

bull Capturing Measuring Experience

bull Future Interface Paradigms

bull Experience Orientated Thinking

bull Digital Innovationbull Foresight amp Institutional

Changebull Policies for Change

MOLECULAR DIAGNOSTICS ndash WHO WE ARE

We are a team of 33 scientists and ca 25 PhD and diploma students and our labs are located in Vienna

We have expertise and background in the areas of biology biochemistry bioinformatics physics chemistry electrical engineeringhellip

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY

OWNERSHIP STRUCTURE

REPUBLIC OF AUSTRIA (through the Federal Ministry for Transport Innovation and Technology)

FEDERATION OF AUSTRIAN INDUSTRIES

5046

4954

EMPLOYEES

1300

77 m EUR Contract research revenues (incl Grants)45 m EUR bmvit funding21 m EUR Other operating income

incl Nuclear Engineering Seibersdorf

TOTAL REVENUES

143 m EUR

NuclearEngineering Seibersdorf

GmbH

SeibersdorfLabor GmbH

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY

AIT Austrian Institute of Technology

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

AIT Austrian Institute of Technology

Mobility Systems Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

FOCUS OF THE CENTERS

bull Sustainable Thermal Energy Systems

bull Electric Energy Systemsbull Sustainable Buildings and

Citiesbull Photovoltaic Systemsbull Environmental Resources

amp Technologies

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

Mobility Systems

Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

bull Bioresourcesbull Molecular Diagnosticsbull Biomedical Systemsbull Digital Health Information

Systems

bull Security amp Communication Technologies

bull Visual Surveillance and Insight

bull Smart Sensor Solutionsbull Dependable Systems

Engineeringbull Information Management

bull High-Performance Image Processing

bull Autonomous Systemsbull Complex Dynamical

Systems

bull Transportation Infrastructure Technologies

bull Dynamic Transportation Systems

bull Electric Drive Technologies

bull Light Metals Technologies Ranshofen

bull Capturing Measuring Experience

bull Future Interface Paradigms

bull Experience Orientated Thinking

bull Digital Innovationbull Foresight amp Institutional

Changebull Policies for Change

MOLECULAR DIAGNOSTICS ndash WHO WE ARE

We are a team of 33 scientists and ca 25 PhD and diploma students and our labs are located in Vienna

We have expertise and background in the areas of biology biochemistry bioinformatics physics chemistry electrical engineeringhellip

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

NuclearEngineering Seibersdorf

GmbH

SeibersdorfLabor GmbH

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY

AIT Austrian Institute of Technology

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

AIT Austrian Institute of Technology

Mobility Systems Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

FOCUS OF THE CENTERS

bull Sustainable Thermal Energy Systems

bull Electric Energy Systemsbull Sustainable Buildings and

Citiesbull Photovoltaic Systemsbull Environmental Resources

amp Technologies

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

Mobility Systems

Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

bull Bioresourcesbull Molecular Diagnosticsbull Biomedical Systemsbull Digital Health Information

Systems

bull Security amp Communication Technologies

bull Visual Surveillance and Insight

bull Smart Sensor Solutionsbull Dependable Systems

Engineeringbull Information Management

bull High-Performance Image Processing

bull Autonomous Systemsbull Complex Dynamical

Systems

bull Transportation Infrastructure Technologies

bull Dynamic Transportation Systems

bull Electric Drive Technologies

bull Light Metals Technologies Ranshofen

bull Capturing Measuring Experience

bull Future Interface Paradigms

bull Experience Orientated Thinking

bull Digital Innovationbull Foresight amp Institutional

Changebull Policies for Change

MOLECULAR DIAGNOSTICS ndash WHO WE ARE

We are a team of 33 scientists and ca 25 PhD and diploma students and our labs are located in Vienna

We have expertise and background in the areas of biology biochemistry bioinformatics physics chemistry electrical engineeringhellip

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

FOCUS OF THE CENTERS

bull Sustainable Thermal Energy Systems

bull Electric Energy Systemsbull Sustainable Buildings and

Citiesbull Photovoltaic Systemsbull Environmental Resources

amp Technologies

Energy Health ampBioresources

Digital Safety amp Security

Vision Automation amp Control

Mobility Systems

Low-EmissionTransport

Technology Experience

Innovation Systems amp Policy

bull Bioresourcesbull Molecular Diagnosticsbull Biomedical Systemsbull Digital Health Information

Systems

bull Security amp Communication Technologies

bull Visual Surveillance and Insight

bull Smart Sensor Solutionsbull Dependable Systems

Engineeringbull Information Management

bull High-Performance Image Processing

bull Autonomous Systemsbull Complex Dynamical

Systems

bull Transportation Infrastructure Technologies

bull Dynamic Transportation Systems

bull Electric Drive Technologies

bull Light Metals Technologies Ranshofen

bull Capturing Measuring Experience

bull Future Interface Paradigms

bull Experience Orientated Thinking

bull Digital Innovationbull Foresight amp Institutional

Changebull Policies for Change

MOLECULAR DIAGNOSTICS ndash WHO WE ARE

We are a team of 33 scientists and ca 25 PhD and diploma students and our labs are located in Vienna

We have expertise and background in the areas of biology biochemistry bioinformatics physics chemistry electrical engineeringhellip

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

MOLECULAR DIAGNOSTICS ndash WHO WE ARE

We are a team of 33 scientists and ca 25 PhD and diploma students and our labs are located in Vienna

We have expertise and background in the areas of biology biochemistry bioinformatics physics chemistry electrical engineeringhellip

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

MOLECULAR DIAGNOSTICS ndash WHAT WE DO

We identify and validate useful biomarkers in minimally or non-invasive body fluids We create point-of care solutions to measure biomarkers in those fluids

Together with our clinical and industrial partners we create technical solutions for applied questions

We have a special focus on saliva as an absolutely non-invasive sample type that is ideally suited for point-of care measurements

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

Non-invasiveDiagnostics

Biomarker Development

Assay Development

DiagnosticBiosensors

Systems Integration Point-of-Care

Bioinformatics

OUR TECHNICAL CORE COMPETENCIES

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

bull DNAbull Genotypingbull DNA-methylationbull Genomic Aberrations

bull RNAbull Gene Expressionbull miRNAbull ncRNA

bull Proteinbull auto antibody profiling

bull DNA Microarraybull whole genomebull targeted

bull Next Generation Sequencingbull qPCRbull Microfluidic qPCRbull Protein Arraysbull Peptide Arraysbull Biostatisticsbull Bioinformaticsbull Software Development

Technologies

BIOMARKER DEVELOPMENT - EXPERTISE

Thyroid CancerBreast Cancer Lung Cancer LeukemiasInfectiousDisease

Fibroprolif Diseases

Indications

Thyroid CaBreast Cancer Lung Cancer LeukemiasColon Cancer Fibroprolif Diseases

Aim Improving minimal invasive diagnostics

Prostate Ca

DNA Methylation

Auto Antibody Profiling

Biomolecules

BIOINFORMATICS ndash EXPERTISE AND OVERVIEW

Molecular Data

Biostatistics Diagnostics

bull Clinical Needbull Experimental Designbull Operating Conditions

Assay Design Validation Studies Software Design

Clinical Data Study Design

Domain specific knowledgebull NGSbull Chipsbull HT-qPCRbull Luminexbull hellip

Disease model characterisation

bull Feature Selection andbull Classification under bull Operating Conditions (cost structures)bull On longitudinal databull Meta-analysis

bull Whole genome analysisbull Data integrationbull Meta-analysisbull Gene Set Enrichmentbull Regulatory networksbull Hypothesis generation

bull Genomicsbull Epigenomicsbull Transcriptomicsbull Immunomics

Proteomics

ASSAY DEVELOPMENT - SERVICES FORIMMUNOLOGICAL AND BIOCHEMICAL ASSAYS

Assay Development amp Validationbull QCbull Performance improvementbull POC adaptionbull Saliva adaptionbull Total assay automation

Routine Sample Measurement Available Assay Technologies

bull FIbull UVbull FRETbull HTRFbull Lancebull Delfiabull Luminescence (Flash amp Glow)bull Alpha ScreenLisabull Enzyme kinetics (Seconds to minutes to hours)

BIOSENSOR DEVELOPMENT ndash OVERVIEW

Peptides(eg hormones)

Proteins(eg antibodies

enzymes hormones)

Nucleic acids(DNA RNA

mRNA miRNA)

Viruses cells

Waveguides

Surface plasmonresonance (SPR)

Fluorescence

Chemo-luminescenceWhite light interferometry

Magneticnanoparticles

Magneto-resistance

Micro-nuclear magneticresonance

Colorimetric(lateral flow devices)

Quartz crystal microbalance

Surfaceacoustic waves

Amperometry

Voltammetry

Impedancespectroscopy Cantilevers

Optical Magnetic Electro-chemical

Massbased

Waveguides Magneticnanoparticles

Biological targets

Sample liquid

Signal readoutamp processing

Transducer

Receptor surface

Biosensor

Display of results

Greensense IMPETUS NUMBAT PathoPOC PIONIER

COHESION COLODOR OCTCHIP PASSION PLASMOfab POCbosens

LAMPION

Current national and international projects

SYSTEMS INTEGRATION POINT OF CARE DEVICES -EXPERTISE AND OVERVIEW

Major requirements Rapid analysis lt30min Compact systems Automation Cost-effective analysis

3 topics Sample preparation with

electrical fields Miniaturised immunoassays Veterinary diagnostics

Total Analysis Systems

Biomarker

Biosensors

Fluidic handling amp

storage

Processcontrol amp

Signal acquisition

Externaltechnology

Sample pre-paration

Bio-informatics

Data processing

AssayDevelop

MAJOR RESEARCH PROGRAMMES - OVERVIEW

BiosensorsPoint-of-Care

Biomarker andAssay Development

Bioinformatics

bull DNA-methylation miRNA and autoantibody biomarkers in blood and saliva (eg cancer diabetes)

bull New technical platforms for peptide based immunomics

bull Pre-analytics of body fluidsbull Blood and saliva based

detection of pathogens

Funding (eg)Ultraplacad (H2020 PHC10)FAPIC (H2020 PHC10)MARA (H2020 Fet-Open)

bull Optical magnetical and electrochemical technologies for biosensors

bull Printing technologies for electrode systems in the POC

bull Integration of samples prep and detection technologies into POC solutions for non-invasive diagnostics

Funding (eg)Diagoras (H2020 PHC10)OCTchip (H2020 ICT27)PlasmoFAB (H2020 ICT27)

bull bdquoBioinformatic partldquo of many projects in the biomarker area

bull New workflows for data handling from different NGS-platforms with a specific focus on RNAseq

bull Statistic interpretation and evaluation for specific molecular diagnostic questions

Funding (eg)Libida (FFG Bridge)Druid (ZIT)CBMed (FFG K1 Center)

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

ASSAY DEVELOPMENT - SERVICES FORIMMUNOLOGICAL AND BIOCHEMICAL ASSAYS

Assay Development amp Validationbull QCbull Performance improvementbull POC adaptionbull Saliva adaptionbull Total assay automation

Routine Sample Measurement Available Assay Technologies

bull FIbull UVbull FRETbull HTRFbull Lancebull Delfiabull Luminescence (Flash amp Glow)bull Alpha ScreenLisabull Enzyme kinetics (Seconds to minutes to hours)

BIOSENSOR DEVELOPMENT ndash OVERVIEW

Peptides(eg hormones)

Proteins(eg antibodies

enzymes hormones)

Nucleic acids(DNA RNA

mRNA miRNA)

Viruses cells

Waveguides

Surface plasmonresonance (SPR)

Fluorescence

Chemo-luminescenceWhite light interferometry

Magneticnanoparticles

Magneto-resistance

Micro-nuclear magneticresonance

Colorimetric(lateral flow devices)

Quartz crystal microbalance

Surfaceacoustic waves

Amperometry

Voltammetry

Impedancespectroscopy Cantilevers

Optical Magnetic Electro-chemical

Massbased

Waveguides Magneticnanoparticles

Biological targets

Sample liquid

Signal readoutamp processing

Transducer

Receptor surface

Biosensor

Display of results

Greensense IMPETUS NUMBAT PathoPOC PIONIER

COHESION COLODOR OCTCHIP PASSION PLASMOfab POCbosens

LAMPION

Current national and international projects

SYSTEMS INTEGRATION POINT OF CARE DEVICES -EXPERTISE AND OVERVIEW

Major requirements Rapid analysis lt30min Compact systems Automation Cost-effective analysis

3 topics Sample preparation with

electrical fields Miniaturised immunoassays Veterinary diagnostics

Total Analysis Systems

Biomarker

Biosensors

Fluidic handling amp

storage

Processcontrol amp

Signal acquisition

Externaltechnology

Sample pre-paration

Bio-informatics

Data processing

AssayDevelop

MAJOR RESEARCH PROGRAMMES - OVERVIEW

BiosensorsPoint-of-Care

Biomarker andAssay Development

Bioinformatics

bull DNA-methylation miRNA and autoantibody biomarkers in blood and saliva (eg cancer diabetes)

bull New technical platforms for peptide based immunomics

bull Pre-analytics of body fluidsbull Blood and saliva based

detection of pathogens

Funding (eg)Ultraplacad (H2020 PHC10)FAPIC (H2020 PHC10)MARA (H2020 Fet-Open)

bull Optical magnetical and electrochemical technologies for biosensors

bull Printing technologies for electrode systems in the POC

bull Integration of samples prep and detection technologies into POC solutions for non-invasive diagnostics

Funding (eg)Diagoras (H2020 PHC10)OCTchip (H2020 ICT27)PlasmoFAB (H2020 ICT27)

bull bdquoBioinformatic partldquo of many projects in the biomarker area

bull New workflows for data handling from different NGS-platforms with a specific focus on RNAseq

bull Statistic interpretation and evaluation for specific molecular diagnostic questions

Funding (eg)Libida (FFG Bridge)Druid (ZIT)CBMed (FFG K1 Center)

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

BIOSENSOR DEVELOPMENT ndash OVERVIEW

Peptides(eg hormones)

Proteins(eg antibodies

enzymes hormones)

Nucleic acids(DNA RNA

mRNA miRNA)

Viruses cells

Waveguides

Surface plasmonresonance (SPR)

Fluorescence

Chemo-luminescenceWhite light interferometry

Magneticnanoparticles

Magneto-resistance

Micro-nuclear magneticresonance

Colorimetric(lateral flow devices)

Quartz crystal microbalance

Surfaceacoustic waves

Amperometry

Voltammetry

Impedancespectroscopy Cantilevers

Optical Magnetic Electro-chemical

Massbased

Waveguides Magneticnanoparticles

Biological targets

Sample liquid

Signal readoutamp processing

Transducer

Receptor surface

Biosensor

Display of results

Greensense IMPETUS NUMBAT PathoPOC PIONIER

COHESION COLODOR OCTCHIP PASSION PLASMOfab POCbosens

LAMPION

Current national and international projects

SYSTEMS INTEGRATION POINT OF CARE DEVICES -EXPERTISE AND OVERVIEW

Major requirements Rapid analysis lt30min Compact systems Automation Cost-effective analysis

3 topics Sample preparation with

electrical fields Miniaturised immunoassays Veterinary diagnostics

Total Analysis Systems

Biomarker

Biosensors

Fluidic handling amp

storage

Processcontrol amp

Signal acquisition

Externaltechnology

Sample pre-paration

Bio-informatics

Data processing

AssayDevelop

MAJOR RESEARCH PROGRAMMES - OVERVIEW

BiosensorsPoint-of-Care

Biomarker andAssay Development

Bioinformatics

bull DNA-methylation miRNA and autoantibody biomarkers in blood and saliva (eg cancer diabetes)

bull New technical platforms for peptide based immunomics

bull Pre-analytics of body fluidsbull Blood and saliva based

detection of pathogens

Funding (eg)Ultraplacad (H2020 PHC10)FAPIC (H2020 PHC10)MARA (H2020 Fet-Open)

bull Optical magnetical and electrochemical technologies for biosensors

bull Printing technologies for electrode systems in the POC

bull Integration of samples prep and detection technologies into POC solutions for non-invasive diagnostics

Funding (eg)Diagoras (H2020 PHC10)OCTchip (H2020 ICT27)PlasmoFAB (H2020 ICT27)

bull bdquoBioinformatic partldquo of many projects in the biomarker area

bull New workflows for data handling from different NGS-platforms with a specific focus on RNAseq

bull Statistic interpretation and evaluation for specific molecular diagnostic questions

Funding (eg)Libida (FFG Bridge)Druid (ZIT)CBMed (FFG K1 Center)

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

SYSTEMS INTEGRATION POINT OF CARE DEVICES -EXPERTISE AND OVERVIEW

Major requirements Rapid analysis lt30min Compact systems Automation Cost-effective analysis

3 topics Sample preparation with

electrical fields Miniaturised immunoassays Veterinary diagnostics

Total Analysis Systems

Biomarker

Biosensors

Fluidic handling amp

storage

Processcontrol amp

Signal acquisition

Externaltechnology

Sample pre-paration

Bio-informatics

Data processing

AssayDevelop

MAJOR RESEARCH PROGRAMMES - OVERVIEW

BiosensorsPoint-of-Care

Biomarker andAssay Development

Bioinformatics

bull DNA-methylation miRNA and autoantibody biomarkers in blood and saliva (eg cancer diabetes)

bull New technical platforms for peptide based immunomics

bull Pre-analytics of body fluidsbull Blood and saliva based

detection of pathogens

Funding (eg)Ultraplacad (H2020 PHC10)FAPIC (H2020 PHC10)MARA (H2020 Fet-Open)

bull Optical magnetical and electrochemical technologies for biosensors

bull Printing technologies for electrode systems in the POC

bull Integration of samples prep and detection technologies into POC solutions for non-invasive diagnostics

Funding (eg)Diagoras (H2020 PHC10)OCTchip (H2020 ICT27)PlasmoFAB (H2020 ICT27)

bull bdquoBioinformatic partldquo of many projects in the biomarker area

bull New workflows for data handling from different NGS-platforms with a specific focus on RNAseq

bull Statistic interpretation and evaluation for specific molecular diagnostic questions

Funding (eg)Libida (FFG Bridge)Druid (ZIT)CBMed (FFG K1 Center)

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

MAJOR RESEARCH PROGRAMMES - OVERVIEW

BiosensorsPoint-of-Care

Biomarker andAssay Development

Bioinformatics

bull DNA-methylation miRNA and autoantibody biomarkers in blood and saliva (eg cancer diabetes)

bull New technical platforms for peptide based immunomics

bull Pre-analytics of body fluidsbull Blood and saliva based

detection of pathogens

Funding (eg)Ultraplacad (H2020 PHC10)FAPIC (H2020 PHC10)MARA (H2020 Fet-Open)

bull Optical magnetical and electrochemical technologies for biosensors

bull Printing technologies for electrode systems in the POC

bull Integration of samples prep and detection technologies into POC solutions for non-invasive diagnostics

Funding (eg)Diagoras (H2020 PHC10)OCTchip (H2020 ICT27)PlasmoFAB (H2020 ICT27)

bull bdquoBioinformatic partldquo of many projects in the biomarker area

bull New workflows for data handling from different NGS-platforms with a specific focus on RNAseq

bull Statistic interpretation and evaluation for specific molecular diagnostic questions

Funding (eg)Libida (FFG Bridge)Druid (ZIT)CBMed (FFG K1 Center)

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

OUR PATENT PORTFOLIO

Currently we actively pursue and market the following 17 patent families

Breast cancer autoantibody biomarker Colon cancer autoantibody biomarker Lung cancer autoantibody biomarker Prostate cancer autoantibody biomarker Lung cancer DNA-methylation biomarker Breast cancer DNA-methylation biomarker Colon cancer DNA-methylation biomarker Thyroid cancer DNA-methylation biomarker Thyroid cancer m-RNA expression biomarker Rotating nanowires surface plasmon detection technology Cellmonitor wireless signal transfer technology New DNA probe assay technology Microfluid detection system for bed-side molecular diagnosis Selective electrical cell lysis systems New molecular diagnostic technologies (MARA 3 patent families)

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

OUR TECHNOLOGY MODULE APPROACH

In our cooperative as well as in our industry projectswe usually act as a provider of technology modulesby eg

bull Integrating customers biomarker content onto our own POC platformsbull Contributing our own content to existing platforms of customers bull Identifying and developing the ideal sensor solution for partnersbull Contributing our bioinformatics expertise for evaluation of large datasets bull Integrating all components into complete POC systems

In selected projects we are also developing completely new and revolutionary diagnostic technology modules (eg H2020 FET-Open project MARA)

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

OUR LABORATORIES ndash INFRASTRUCTURE

Molecular Biology Laboratories Modern Molecular Biology Laboratories S2 Laboratory PCR Laboratory Microarray Printing High Throughput Systems (eg NGS)

Chemistry Laboratory

Physics Laboratory

Clean Room and CTFT (Center of Thin Film Technology) Magnetron Sputtering Materials Inkjet Printer Optical lithography Atomic Force Microscopy

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

OUR PARTNERS

Some Major Academic Partners Some Major Industry Partners

Medical University Vienna

Vetmed University Vienna

UCLA

Queensland University of Technology

Abcodia

Infineon

Boehringer Ingelheim

ams

NXT-Dx

PlatomicsHahn SchickardGesellschaft (HSG)

Joanneum Research Weiz

AIT AUSTRIAN INSTITUTE OF TECHNOLOGYyour ingenious partner

Dr Martin JungHead of Competence UnitMolecular DiagnosticsCenter for Health amp Bioresources

mjungaitacat

• MoleculaR dIAGNOSTICS
• AIT Austrian institute of technology
• AIT Austrian institute of technology
• Focus of the centers
• Molecular Diagnostics ndash Who we are
• Molecular Diagnostics ndash What we do
• Our Technical Core Competencies
• Foliennummer 8
• Bioinformatics ndash Expertise and overview
• Foliennummer 10
• Biosensor development ndash overview
• Foliennummer 12
• Major Research Programmes - Overview
• Our Patent Portfolio
• Our Technology Module Approach
• Our Laboratories ndash Infrastructure
• Our Partners
• AIT Austrian Institute of Technology