algal control by phosphate restriction
DESCRIPTION
Algal Control By Phosphate Restriction. Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate. Newport Back Bay is nearby and may also be contaminated with the toxic algae. Eastlake (Yorba Linda) is the control lake and is not being treated. - PowerPoint PPT PresentationTRANSCRIPT
Algal Control By Phosphate Restriction
Mason Lake (Irvine) contains toxic algae. They will install a water treatment plant to remove phosphate.
Newport Back Bay is nearby and may also be contaminated with the toxic algae.
Eastlake (Yorba Linda) is the control lake and is not being treated.
Mason Lake
Sampling Site
Toxic Algae in Mason Lake
Visibility: 4cm
High phosphate levels are from many water fowl
Toxin-Producing Microcystis sp.
Mason Lake: One Mile From Newport Back Bay Estuary
Newport Back Bay
Urban runoff into the bay
Bay
Storm drains with Phosphate-rich water from urban run-off
Phosphate-rich water dumping into the Bay
Upper Newport Bay
Top birding site Home to 200 species of birds 30,000 migrating birds per day Audubon Society/F&G conducts a
monthly bird count
Endangered Species at Newport Back Bay: California Least Tern
Light-Footed Clapper Rail
Brown Pelican
Peregrine Falcon
Belding’s Savannah Sparrow
Coastal California Gnatcatcher
East Lake (Yorba Linda)Sampling Site
East Lake: Yorba Linda, CA
Algae is creating a foamy surface scum
A Diatom called Navicula is causing the foam
Sample Analysis
Algal Assay Species ID Cell count
Chlorophyll Assay Flow Cytometry
Species populations Phosphate Assay
Algal Assay
Eastlake: Diatoms
Mason Lake: Cyanobacteria
Flow Cytometry is used to count algal cells and separate them by species
Flow Cytometry is used to count algal cells and separate them by species
Chlorophyll Assay
Filter the water to capture the algae on the filter paper
Mason Lake Eastlake
Grind up filter paper with acetone to release chlorophyll
After Centrifuge
Filter algae out of water, add a reagent that turns blue when it contacts phosphate
Phosphate Assay
Mason Lake Eastlake
Filters with algae left on them
The deeper the blue color, the
more phosphate is present.
A dilution series with phosphate standard