allan green - csiro research · csiro business units and facilities. agriculture & food....
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Opening Comments: CSIRO introductionGene editing in the context of metabolic engineering
Allan GreenInnovation Leader – Biobased ProductsCSIRO Agriculture & Food
CSIRO – Australia’s innovation catalyst
5486talented staff
$1billion+ budget
Workingwith over2800+industry partners
55sites across
Australia
Top 1%of global research agencies
Each year6 CSIRO
technologies contribute
$5 billion tothe economy
CSIRO Business Units and FacilitiesAgriculture & Food
Energy
Health and Biosecurity
Land and Water
Manufacturing
Mineral Resources
Oceans & Atmosphere
Astronomy andSpace Science
Australian Animal Health Laboratory
Data61
Marine National Facility
National Computing Infrastructure
National Research Collections of Australia
National facilities and collections
We managestate-of-the art
facilities on behalf of Australia
Impact science
We solve the biggest
challenges facing our nation and the world
GLOBAL PRECINCTSNatural & environmental sciences | ACTNational resource sciences | WAEcoSciences | QLDHuman life sciences | VICManufacturing & materials innovation | VIC
MAJOR INFRASTRUCTUREASKAP radio telescope | WAThe Pawsey supercomputing centre | WAMarine research vessel | TAS
Strengthening our national connections
CSIRO Future Science Platforms
• Capacity development in areas of identified future importance for Australia
– Tied to Strategy 2020
• $75 M investment (multi-year)– Expected to build to 10-12% of strategic funds
• Build next generation of researchers• Interdisciplinary, cross-business unit programs
Frontier science to help reinvent and create new industries for Australia
Active Integrated Matter Big data, advanced autonomous systems and materials science
Probing BiosystemsReal-time interrogation of living biological systems
EnvironomicsReinvent how we measure and monitor ecosystems
Deep Earth ImagingPrecisely image subsurface
rock properties at depth
DigiscapeNext generation decision support
tools to transform agricultural
Synthetic BiologyBuild capability in synthetic biology
across CSIRO and Australia
Application Domains
Foundation TechnologiesBuild the tools required to deliver synbio solutions across a range of systems
Chemicals & FibresDeliver substantial, disruptive technologies for our chemical and fibre industries
Environment & BiocontrolRevolutionise our capacity to control and remediate our environment
HealthAdvance medical breakthroughs and deliver new health innovations
Underlying Technical/Science Domains
Integrative Biological ModellingPredictive modelling of biological systems
Maximising Impact Social, ethical, regulatory considerations to deliver SynBio solutions
Assembling Novel BiosystemsEngineered in vitro and organismal (eco)systems
Engineering Novel Biological ComponentsParts; non-model chasses; support capabilities
Accelerating challenges …
Integrated solutions are urgent
… enabling technology needs to keep pace
Accelerating challenges …
Integrated solutions are urgent
… 12 yr breeding cycle = 0.75B population !
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Phenotype
Random mutations
Gene discovery
Directed mutation
Metabolic engineering technology development
13 |
Science developments
Non-GM products
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Phenotype
Random mutations
Gene discovery
Directed mutation
Metabolic engineering technology development
14 |
Science developments
Non-GM products
GENE EDITING
Phenotype
Random mutations
Gene discovery
Directed mutation
Metabolic engineering technology development
15 |
Non-GM products
“Flying blind” …• Phenotype target• Pathway uncertain?• Genetics unknown?• 70,000+ plants screened• Off-target removal
Phenotype
Random mutations
Gene discovery
Directed mutation
Metabolic engineering technology development
16 |
Non-GM products
“Flying blind” …• Phenotype target• Pathway uncertain?• Genetics unknown?• 70,000+ plants screened• Off-target removal
Gene editing …• Known target gene• Precise change• Genotype sequencing• For global mutation or
tissue specific genes
?
+ 30 yrs
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
17 |
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
18 |Seed-specific silencing
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
19 |Seed-specific silencing Seed-specific silencing
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
20 |Seed-specific silencing Seed-specific silencing
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
21 |Seed-specific silencing Seed-specific silencing
Gene editing for pathway engineering?
Could we use precise editing of endogenous genes to achieve these products instead of introducing new functional transgenes …
Non-transgenic plants Non GMO products?
Genotype
Gene silencing
Gene addition
Pathway addition
Pathway controls
GM products
Metabolic engineering technology development
23 |Seed-specific silencing Seed-specific silencing