alliance hplc high throughput system€¦ · demand means every second in your system’s analysis...
TRANSCRIPT
P roduct iv it y. P recision. reliabil it y.
Today’s pharmaceutical laboratories are charged with moving thousands of samples through drug
discovery screening processes, all while maximizing return on investment in both time and resources.
You depend on automation to increase sample throughput and handle any sample format – tubes,
vials, and/or well plates – all while meeting the need to produce reliable results and identify leads.
Waters understands that when your lab is operating around the clock, keeping up with throughput
demand means every second in your system’s analysis time counts.
The Waters® Alliance® HPLC High Throughput (HT) System with integrated and automated sample
and solvent management is optimized for high efficiency separations, allowing valuable seconds to
be saved from each step of the chromatography process. The Alliance HPLC HT System is specifically
designed to improve the productivity of labs that require the selectivity and sensitivity of LC/MS
to produce high quality data.
The Alliance HPLC HT System features:
n Flexible modes of LC operation (sequential and parallel)
n Sample format versatility – plates, tubes, or vials
n Automated SystemPREP routine for rapid unattended system equilibration
n Purge-wash-purge capability for minimal sample carryover
alliance HPlc HiGH tHrouGHPut syst eM
tHe alliance Ht systeM can be conFiGured WitH:n e2795 separations Module
n optional alliance column
Heater or column
Heater/cooler
n a choice of high performance
optical and/or lc/Ms
detection offerings
n Masslynx™ or empower™
software
n a variety of leading HPlc consumables
The highly reproducible flow rates generated by the
Alliance HPLC HT System make it ideally suited
for coupling with mass spectrometers, such as the
Waters 3100 Mass Detector.
desiGned Wit H eFF ic iency in Mind
From sample prep, analysis, sensitivity, and quantitation hurdles, to uptime, compliance maintenance, and support concerns,
your Alliance HPLC HT System will allow you to meet these challenges and achieve the best performance and productivity for
your laboratory’s requirements.
choice of sample processing modes
Its integrated software and hardware design offers you the choice
of traditional sequential mode or parallel processing. Parallel
sample processing can reduce cycle time by performing multiple
chromatographic and system operations simultaneously. While
one sample is running, the next sample aspirates and loads; it is
immediately injected once the previous sample run is complete.
This accelerated injection-to-injection process greatly reduces cycle
time, and allows more samples to be analyzed in less time.
environmental control and sample stability
Controlling the environmental conditions of samples is
accomplished with a Peltier-based heating and cooling module,
which promotes sample stability, minimizes evaporation, and
stabilizes labile samples. The factory-installed sample heater/
cooler option maintains temperatures from 4 to 40 °C.
auto-process up to 1536 samples
The robotics-based three-axis (X, Y, Z) sample manager provides expanded
sample capacity for processing up to 1536 samples configured in four plates.
The instrument stores pre-programmed coordinates for standard microtiter plates,
or lets you input custom coordinates. To prevent needle damage and allow for
minimal sample volumes, a sensing routine determines the well or vial bottom.
And with rapid, automated initialization, system start-up is quick and easy.
dual solvent wash
The Alliance HPLC HT System utilizes two solvents to purge,
wash, and re-purge the needle assembly, loop, and injection
port. This reduces the risk of sample carryover and assures
assay integrity – critical for ADME and metabolite research –
making it ideal for high-sensitivity LC/MS/MS.
no tools are necessary
Primary components, such
as seals and pistons, can be
maintained by hand.
robust solvent management
The solvent management system blends up to four
chromatographic solvents in precise proportions for smooth,
pulse-free solvent flow delivery. This ensures that over
multiple runs, your results are precise time after time. The
PerformancePLUS™ In-line
Degasser efficiently removes
gases from the chromatographic
solvents. A fifth chamber in
the degasser handles purge
solvent used in the sample
management system.
automated valve switching
Change column methods, run multiple methods, or perform
unattended methods development with automated valve
switching, for up to three or six columns. Columns can be heated
or cooled in a Peltier-based forced air recirculation oven.
MaKinG ev ery second count
From sample processing to solvent management, the Alliance HPLC HT System has been designed to provide significant time savings by
enabling greater throughput along each step in your separation cycle. Key to this feature is being able to choose how you run your samples.
You can maximize throughput while maintaining control of your chromatographic separations. You can select among three processing options:
n sequential – Same as a traditional
chromatography separation cycle – each
function occurs in a step-by-step sequence.
n Parallel – Parallel processing offers an
advantage over traditional (sequential)
chromatography cycle times by performing
chromatographic operations in parallel.
Parallel processing maximizes throughput
by minimizing cycle time. While one sample
is running, a second sample can be drawn
and injected immediately after the preceding
injection is complete.
n Parallel with rapid equilibration – The system
provides for rapid equilibration by switching the
column off-line, purging the system at a high
flow rate, and then loading the next sample
during column re-equilibration. By using
parallel processing with rapid equilibration,
you can significantly increase throughput for
some applications.
Further processing improvements can be achieved by alternating the offline column regeneration. the use of two columns with the
system and a special regeneration valve, while incorporating parallel sampling allows for continuous data collection with no need
for additional re-equilibration time.
inJe
ct
seQuential
inJe
ctDELAY GRADIENT
needle wash
EQUILIBRATION
system and column sample load and draw
inJe
ct
Parallel
inJe
ctDELAY GRADIENT
needle wash
EQUILIBRATION
system and column sample load and draw
40% moresamples tested
inJe
ct
Parallel WitH raPid eQuilibration
inJe
ctGRADIENT
needle wash sample draw
RAPID EQUILIBRATION
sample load equilibrate column delay
Cycle Time = 5.1 min5 minutes run time188 runs in 24 hours
Cycle Time = 6.2 min5 minutes run time154 runs in 24 hours
Cycle Time = 4.4 min3.2 minutes run time218 runs in 24 hours
0 21 3 4 5 6 7 8 9 10 11
Minutes0 21 3 4 5 6 7 8 9 10 11
Minutes
0 21 3 4 5 6 7 8 9 10 11Minutes
Independently driven pistons achieve smooth solvent flow – without pulse dampeners – reducing gradient volume
Dual pressure transducers enable true pulse-free solvent flow
High-efficiency vacuum degassing technology
Precise solvent blending delivers highly reproducible gradients
Tool-free routine maintenance on plungers/seals/seal-wash seals
Simplified flow path with only two inlet check valves
Sample format versatilitySamples can be processed from standard
96 shallow- and deep-well plates, 384-well microtiter plates and custom plates,
2-mL vials, or 0.5- and 1.5-mL microcentrifuge tubes.
int eGrat ed Fluidics For consist ent PerForManc e and enHanc ed reP roducibil it y
The Waters e2795 Separations Module is the core of the system. Its integrated
solvent management and sample management provides consistent performance
and enhanced reproducibility for high-quality, high-throughput analysis for HPLC,
LC/MS, or flow injection-mass spectrometry applications.
solvent management
The simplified design of the e2795 Solvent Manager enables the blending and
delivering of solvents in a pulse-free, low-dispersion flow path.
In addition, Synchronized Composition Control™ (SCC™) of the gradient propor-
tioning valve (GPV) ensures that the e2795 Separations Module delivers excellent
gradient composition accuracy and precision.
The overall design results in reduced complexity and enhanced overall ruggedness
across a flow range that covers two orders of magnitude.
sample management
The design of the e2795 Sample Manager ensures precise sampling – delivering
predictable peak areas, and thus more consistent results. Complimenting the
simplified design of the solvent flow path, a user programmable needle wash, and
selectable “purge-wash-purge” cycle, ensures that any part of the system exposed
to sample is fully washed, virtually eliminating the risk of sample carryover.
The inherent versatility of the three-axis (X, Y, Z) needle assembly of the e2795
Sample Manager allows for greater applications flexibility. The sample manager
has a wide range of sample loops, holding loops, and syringe sizes available and
can process samples from a variety of plates, vials, and/or tubes. This variety
allows you to customize the configuration of the sample manager according to the
needs of your applications.
automated system start-up
The Alliance HPLC HT System features SystemPREP – a programmable, auto-
mated system preparation routine for fast, one-touch, walk-up operation. The
SystemPREP function performs a series of automated steps, putting the separa-
tions module in a condition of complete system readiness for running samples.
SystemPREP is useful when you are changing solvents or after extended periods
of system inactivity and is a simple-to-use alternative to running the individual
functions of Wet Prime, Purge Injector, Equilibrate, and Condition Column.
do More Wit H your results
Waters software solutions enable you to capitalize on the results
generated by your system. The Waters Laboratory Informatics Suite
includes proven information management solutions that integrate
all your lab resources in a consistent, scalable, and seamless
application environment.
Empower Software provides control of the system, including data
acquisition, management, processing, and reporting, all in the market-
leading chromatography data software (CDS) platform. Empower
Software is scalable from a single workstation to an enterprise-wide
CDS solution, and offers advanced features for method development,
reporting, and custom calculations, as well as Enterprise Options
for advanced data management and automated method validation
management.
MassLynx Software, for advanced mass spectrometry applications,
enables researchers to acquire, analyze, manage, and distribute
information. MassLynx Software offers flexible control over complex
instrument configurations, and Application Managers that are tailored
for specific LC/MS analyses.
NuGenesis® SDMS is a customizable scientific information
management platform that automatically captures and stores data in
a centralized data warehouse. SDMS makes information easier to access,
facilitates regulatory compliance, and aids in the administration of
intellectual property. SDMS converts scientific results into accessible
and valuable corporate knowledge.
be assured. cHoose Wat ers Global serv ic es.
With 94 offices in more than 50 countries, only the Waters Global
Services team has the most in-depth and up-to-date knowledge of the
advanced science and technologies that provide the foundation for
Alliance HPLC Systems. Maximize system uptime, increase laboratory
productivity, and meet stringent compliance requirements with the
expertise and responsiveness you need.
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Waters, Alliance, Symmetry, XTerra, and NuGenesis are registered trademarks of Waters Corporation. The Science of What’s Possible, MassLynx, Empower, PerformancePLUS, Synchronized Composition Control, and SCC are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2008 Waters Corporation. Produced in the U.S.A.October 2008 720000781EN LB-PDF