ambr® 15 fermentationNew Microbial Single-UseAdvanced Micro Bioreactor System

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A New Version of the Industry Standard ambr® 15 Micro Bioreactor, for Microbial Screening Applicationsambr 15 fermentation is an automated microscale bioreactor system that is based on the gold standard ambr 15 technology. It provides a consistent microscale model for early stage microbial screening experiments with fed-batch culture capability. This benchtop system comprises disposable micro bioreactor vessels, an automated workstation and user friendly software. It offers automated parallel processing and control of 24 bioreactor experiments by one operator.

Designed for installation in a standard laminar airflow biological safety cabinet for aseptic operation, ambr 15 fermentation provides efficient, consistent and predictive results compared to classical laboratory bioreactors.

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- Increases lab productivity through automation of experiment setup, sampling and reagent addition, for up to 24 experiments in parallel.

- Enhances scalability over shaken cultures

with control of DO & pH in fed-batch culture, impeller stirring, gas sparging and pumped additions of pH reagent and feed.

- Provides predictive screening results early in development

enabling the best early decisions and improving the overall efficiency and timeline of development process.

- Enables data rich culture profiles with automated sampling and enough

culture volume for many samples during a run.

- Reduces the cost per experiment through savings on facility space, capital, labour, media and consumables.

- Provides highly consistent data as proven by the industry standard

‘ambr 15 cell culture’ micro bioreactor platform, now implemented at 90% of the top 20 biopharma.

ambr 15 fermentation transforms the way scientists undertake microbial strain selection and early process development in the 21st century

Features

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Culture stationsEach culture station can run up to 12 micro bioreactor vessels and controls temperature and impeller speed. Gas mixture is delivered independently to each vessel.

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Pumped addition of feed and pH reagents Pump and tubing provide controlled delivery of feed and pH reagents, enabling high density fed-batch cultures with precise pH control.

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Image shows ambr 15 fermentation 24 vessel system

ambr 15 fermentation combines disposable micro bioreactor vessels, automated workstation and easy-to-use software

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Disposable tipsTwo sizes of high grade sterile disposable tips are available to transfer liquid volumes accurately and precisely.

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Individual vessel temperature control Sensors and heaters for each vessel provide a steady, uniform culture temperature, even with a range of growth rates and heat outputs in different vessels.

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Labware lid managementLids are automatically removed prior to liquid transfer and replaced afterwards.

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SoftwareSoftware enables easy experiment construction and controls and monitors all experiments, with full audit trail.

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Liquid handling unitFor media, feed and reagent addition plus sampling from the bioreactor vessels. Unit includes dual pipette heads, automated vessel decapper and plate delidder.

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Chilled plate position Chilled storage of media or culture samples provides flexibility in scheduling of user interactions. Chilled sample storage facilitates data rich culture profiles with multiple samples during a run.

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ambr 15 fermentation – systemFully automated parallel processing, control and evaluation of all bioreactorexperiments with:

– Online monitoring and closed-loop control of pH (6-8) and DO.

– Cascade DO control system for gas flow, stirrer speed and gas mixture.

– Independent control of N2, air and O2 for each vessel.

– Full control of impeller speed, gas flow rates and bioreactor temperature.

Micro bioreactor vesselThe core disposable micro bioreactor technology includes sensors, stirring impeller, gas sparging, fluid additions and sample port.

1. Gas sparge tube2. pH and feed tubes3. Sample port4. pH and DO sensor spots5. Impeller

ambr 15 fermentation – micro bioreactor vesselMimics the characteristics of a classical lab scale bioreactor to enable good early stage predictions of cell growth and productivity:

– pH and DO sensors for continuous monitoring and control (pH 6-8).

– Integral impeller for rapid, efficient mixing.

– Sparge tube delivers gas to the impeller mixing zone.

– 10 to 15 mL working volume (8 to 12 mL liquid plus gas bubbles) with sample port to allow addition of reagents & feeds or removal of samples.

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Functions

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ambr 15 fermentation automatically controls, feeds and samples 24 bioreactor vessels in parallel

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- Vector screening

- Strain selection

- Media development

ambr 15 fermentation can be used as a microscale model to enhance results in a range of development applications such as:

Applications

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Sales and Service ContactsFor further contacts, visit www.sartorius-stedim.com

www.sartorius-stedim.com

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