amino transfer as e

8/17/2019 Amino Transfer as e

1/17

AMINOTRANSFERASE subkela

ENZIM ALANINTRANSFERASEKELOMPOK 2.2 :

FARIS HARDITYA PURNAMA 2100110!00"2

MUHAMMAD FAHRIL 210011#0!000

$ONITA MAHARDIKA PUTRI 210011#0!00#0

MUKHAROMAH EKA NIN%TYAS 210011#0!00


2/17

ENZIM

E&'() a*ala+ sua,u -/,e(& a& *a-a, be,(&*ak sebaa( ka,al(s -b(/k()(a( *( *ala) /a&(s)e +(*u- *a& )e)(l(k( s(3a,4s(3a, a&Sebaa( ka,al(s6 e&'() )e)-e7e-a, la8u eaks( se)ua )e,ab/&a)u& *(a ,(*ak beuba+ sela)a -/ses k()(a( bela&su&.


3/17

KLASIFIKASI ENZIM

E&'() *(klas(5kas(ka& /le+ Nomenclature Committee of the InUnion of Biochemistry and Molecular Biology 9N4IU;M;< be*aseaks(&a ke *ala) e&a) kelas6 a(,u :

1. Ta&s3ease

2. H(*/lase

. L(ase

#. Is/)ease

". L(ase

!. Oks(*/e*uk,ase


4/17

ENZIM TRANSFERASE

E&'() ,a&s3ease a*ala+ e&'() a& (ku, se,a *ala) eaks( -9,a&s3e< sua,u a*(kal a,au uus. E&'() (&( ,el(ba, *ala) )eu- 3u&s(/&al a&,aa */&/ *e&a& akse-,/. /&,/A)(&/,a&s3ease :

1. A)(&/,a&s3ease 9,a&sa)(&ase


5/17

SU;KELAS ENZIM TRANSFER

Ta&s3ease )e&ka,al(s(s -e)(&*a+a& uusa& )/lsua,u )/lekul ke )/lekul a& la(&. Sebaa( 7/&,/bebea-a e&'() sebaa( be(ku,:

1. A)(&/,a&s3ease9Ta&sa)(&ase


6/17

Su)be e&'() Ta&s3ease

Ta&s3ease *a-a, *(-e/le+ *a( +a,( )bak,e(6 *a& +ea&. 7/&,/+ a)(&/,a&s3ease *a-a, *(-e/le+ )elalu(

E.coli *e&a& -((*/ksal "> 3/s3a, sebaa( k/

Dala) (b/s/).DNA -/l()ease *a-a, *(,e)uka& -a*a )+(*u- -/ka(/, )au-u& euka(/,.


7/17

A-l(kas( e&'() Ta&s3ease

Ta&s3ease )e)(l(k( bebea-a a-l(kas( *ala) kese+a,a&AST 9as-a,a, ,a&sa)(&ase< a,au S%OT 9seu) /?al/ase,a, ,a&sa)(&ase< *a& ALT 9ala&(& ,a&s3eaS%PT 9seu) lu,a)a, -(u@a, ,a&sa)(&ase< k/&se&,as(&a *( *ala) *aa+ )e&(&ka, *a-a, )e&ba+a sese/a& )e&ala)( keusaka& +a,(.

DNA -/l()ease *a-a, *(u&aka& -a*a )e,/*e PR. S4,a&s3ease *a-a, *(u&aka& *ala) -/ses *e,se&aa elek,/5l(k ?e&/b(/,.


8/17

AMINOTRANSFERASE

A)(&/,a&s3ease 9,a&sa)(&ase< a*ala+ e&'() a& */le+ +a,( a& )e&ka,al(s(s ,a&s3e asa) a)(&/.

/&,/+ *a( sub kelas e&'() a)(&/,a&s3ease a(, Ta&sa)(&ase a,au Ala&(& A)(&/,a&s3ease a,au P(u@a, Ta&sa)(&ase.

Su)be&a beasal *a( /a&(s)e -/ka(/,(k

euka(/,(k6 ,e)asuk +ea&6 ,u)bu+a&6 *a& )a&us(a.


9/17

ALANINTRANSFERASE

Nama sistematik: L-Alanin:2-oxoglutarataminotransferase.

Ko-Enzim: Piridoksal posfat (Vitamin !"

#u$strat: L-alanin dan 2-oxoglutarat

Produk: Piru%at dan L-glutamat

&ekanisme kinetik: ping-pong bi bi mechanism

ALT 9Ala&(& Ta&sa)(&ase< )e&,a&s3e uus a)(& *a( ala&(& ke 4ke,/)e&8a*( lu,a)a,e6 ala&(& se&*(( beuba+ )e&8a*( -(u@a, *a& *e)(k(a&sebal(k&a. P/ses (&( *(-ea&,aa( /le+ a*a&a k/e&'() -((*/ksal 3/s3a,9@(,a)(& ;!< a& beuba+ )e&8a*( -((*/ksa)(&.


10/17

K/e&'() *a( A)(&/,a&s3ease Koenzim adalah senyawa-senyawa non-protein ya

terdialisa, termostabil dan terikat secara “longgar” dengaprotein dari enzim (apoenzim). Kae&a ,e(ka, se7aa l/

e@es(bel6 ka*a&4ka*a& k/e&'() *(sebu, 8ua k/4subs,a,. k/e&'() )eu-aka& @(,a)(& a,au ,uu&a&&a6 a&,aa la(& @9,(a)(&


11/17

e&(s e&'() *a(A)(&/,a&s3ease

erdasarkan letaknya terhadap sel

E&'() A)(&/,a&s3ease ,e)asuk e&'() (&,aselule kae&a )ba+a& selule *a& )e&ua(ka& &u,(e& u&,uk )e&e*(aka& e&*(bu,u+ka& sel. E&'() (&( ,e)asuk -las)a &/&3u&s(/&al (&,aseE&'() (&( *(+as(lka& /le+ sel4sel /a& ,e,e&,u *a& beke8a *( ,esebu, *ala) -/ses )e,ab/l(s)e6 se-e,( ala&(& ,a&sa)(&as,a&sa)(&ase6 a,au lak,a, *e+(*/e&ase.

erdasarkan si$at kimianya

E&'() A)(&/,a&s3ease ,e)asuk abu&a& a&,aa -/,e(& *e&ak()(a( la(&&a6 uus k()(a ,esebu, beu-a uus A)(&a 9NH2


12/17

Susu&a& E&'()A)(&/,a&s3ease

Ls4A)(&/ C P((*/ksal 3/s3a, 444A)(&/,a&s3ease


13/17

Susu&a& E&'()A)(&/,a&s3ease


14/17

S,uk,u M/lekul P/,e(& *a(A)(&/,a&s3ease

A)(&/,a&s3ease )eu-aka& e&'() a& )e)(l(k( s,uk,u ,es(e kae&a abu&a& a&,aa s,uk,u seku&*e a& )e)be&,uk (ka,a& la(.


15/17

Meka&(s)e Ke8a E&'()

R14H9NHC


16/17

Meka&(s)e Ke8a E&'()


17/17

&/0K01"

amino transfer as e

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