a form of energy that travels in waves these waves are a form of electromagnetic radiation. light...
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WHAT IS LIGHT?
A form of energy that travels in waves These waves are a form of
electromagnetic radiation. Light is not the only form of electromagnetic radiation.
HOW DOES LIGHT CHANGE AS IT MOVES THROUGH A SUBSTANCE?
1. Reflection (like the text above)2. Refraction3. Absorption4. Diffraction (you will not need to
remember this, but will see it in more advanced websites)
MATERIAL AND LIGHT
Opaque A substance that blocks all light from traveling
through Light is reflected or absorbed
Transparent Any substance that allows light to easily pass
through without being scattered. Transluscent
Any substance that scatters light as it passes through
THE COMPOUND MICROSCOPE
Eyepiece or OcularBody Tube
Arm
Coarse Adjustment
Fine Adjustment
Nosepiece
Objective Lens
Stage
Diaphragm
Base
Light Source
SIMPLE COMPOUND 1 lens Example:
Magnifying Glass Magnification
Just the one lens
2 lenses Example: Compound
Microscope Magnification
cular
bjective Multiple for total
magnification = 50x
10x
10x
5xX
WHAT YOU SHOULD NEVER DO WITH A MICROSCOPE
Carry it with one hand. Move the ocular to show someone
something. Move the microscope to show someone
something Use the coarse adjustment in high power (will
crack the slide)
STEPS TO USING A MICROSCOPE
1. Place the specimen on a slide.
2. Start with the low power objective (red)
3. Place the slide over the hole of the stage (where light comes through), raise the stage as high as it can go using the coarse adjustment (large knob)
4. Look into the ocular lens (eyepiece) as you move the coarse adjustment to focus the specimen.
5. Use the fine adjustment to focus more precisely on the specimen.
6. If using the higher power (yellow), just move the objective lens into place. Your specimen should be in view. Again use the fine adjustment knob to focus.
WET MOUNT
Place 1 drop of water on the slide Place your specimen on the drop of water Place another drop of water on top of the
specimen Slowly lower the coverslip over the
specimen on a 45 degree angle (to prevent air bubbles)
Do not bend the cover slip, or push down on it.
STAINING A SLIDE
1. Prepare a wet mount.2. Rip off a piece of small paper towel with a
flat edge (if it doesn’t have a flat edge, fold it over to create one)
3. Place a drop of stain along one edge of the cover slip (lets say the left edge)
4. Place the paper towel along the other edge (the right edge) so that it begins to draw the stain across the slide.
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