00 00 2013 07 29 keiser 200944 sdc2
Post on 31-Dec-2015
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0 10 20 300
5
10
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MPP+ (µmol/l)
MP
P+
(pm
ol/m
g
sec)
VC OCT3
70 kDa
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50
100
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verapamil (µmol/l)
% u
pta
ke o
fM
PP
+
OCT3
A.
C.
B.
D.
VC OCT3
Figure S1. Characterization of stably transfected HEK-OCT3 cells. (A) Western blot of OCT3 in HEK-OCT3 and HEK-VC cells. At the molecular mass of approximately 70 kDa, specific signals were detected in HEK-OCT3 cells, which were not detectable in HEK-VC cells. (B) Immunofluorescence analysis of HEK-OCT3- and HEK-VC cells using immunofluorescence microscopy. OCT3 was localized in the plasma membrane of stably transfected HEK-OCT3 cells, whereas no staining was detectable in the HEK-VC cells, scale bar = 20 µm (C) Uptake of MPP+ (mean±SD) and (D) competition of verapamil (0-1000 µmol/l) with the uptake of MPP+ into HEK-OCT3.
Characterization of stably transfected HEK-OCT3 cells. (A) Western blot of OCT3 in HEK-OCT3 and HEK-VC cells. At the molecular mass of approximately 70 kDa, specific signals were detected in HEK-OCT3 cells, which were not detectable in HEK-VC cells. (B) Immunofluorescence analysis of HEK-OCT3- and HEK-VC cells using immunofluorescence microscopy. OCT3 was localized in the plasma membrane of stably transfected HEK-OCT3 cells, whereas no staining was detectable in the HEK-VC cells, scale bar = 20 µm (C) Uptake of MPP+ (mean±SD) and (D) competition of verapamil (0-1000 µmol/l) with the uptake of MPP+ into HEK-OCT3.
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