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Guide for Bio-Rad Products in Food Testing
July 2012
Food Safety
Bio-Rad Laboratories • Food Science
PART 1 – Routine Testing
Preparation of dilutions 3
Total flora 3
Escherichia coli – ISO standards 4
Escherichia coli – Alternative method: RAPID’E.coli 2 Agar 5
3
Coliforms – ISO standards 6
Coliforms – Alternative method: RAPID’E.coli 2 Agar 7
Enterobacteriaceae 8
Mesophilic lactic acid bacteria 9
ContentsGuide for Bio-Rad Products in Food Testing
Food Safety
This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.
It is not a contractual document.
PART 2 – Pathogen Testing
Salmonella – ISO standard 10
Salmonella – Alternative method: RAPID’Salmonella Agar 12
Salmonella – Alternative method:iQ-Check™ Salmonella II real-time PCR Kit 13
Cronobacter spp. – ISO standard 14
Cronobacter spp. – Alternative method: RAPID’Sakazakii Agar 15
Cronobacter spp. – Alternative method:iQ-Check™ Cronobacter spp. real-time PCR Kit 15
Listeria monocytogenes – ISO standards 16
Listeria monocytogenes – Alternative method:RAPID’L.mono Agar 18
Listeria monocytogenes – Alternative method:iQ-Check™ Listeria monocytogenes II real-time PCR Kit 19
Listeria spp. – Alternative method:RAPID’Listeria spp. Agar 20
10
Listeria spp. – Alternative method: RAPID’L.mono Agar 21
Listeria spp. – Alternative method:iQ-Check™ Listeria spp. real-time PCR Kit 21
Escherichia coli O157:H7 – Alternative method:RAPID’E.coli O157:H7 Agar 22
Escherichia coli O157:H7 – Alternative method:iQ-Check™ E.coli O157:H7 real-time PCR Kit 23
Campylobacter spp. – Alternative method:iQ-Check™ Campylobacter real-time PCR Kit 23
Staphylococcus aureus – ISO standards 24
Staphylococcus aureus – Alternative method:RAPID’Staph Agar 25
Sulfite-Reducing bacteria 26
Clostridium perfringens 26
Bacillus cereus 27
Shigella 27
Routine Testing
Preparation of dilutions Total flora
NF EN ISO 6887-1 (09/1999)
Preparation of test samples, initial
suspension and decimal dilutions
for microbiological examination
Part 1: General rules for preparationof dilutions for microbiological
examination
NF EN ISO 4833 (05/2003)
General guidance for the
enumeration of micro-organisms
Colony-count technique at 30°C
Dilution 10-1
(and following dilutions)
m g or v ml of sample
+
9 x m g or 9 x v ml of
Tryptone Salt or Buffered Peptone Water
(and following dilutions)
Enumeration
PCA
Initial suspension
and/or
decimal dilutionsOrdering Information
Catalog # Description
PCA (Plate Count Agar)/Agar
Ready to use
355-4459 100 ml x 6 bottles355-4457 200 ml x 6 bottles356-3989 90 mm x 20 dishesDehydrated
356-4475 500 g
Tryptone-Salt/Diluent
Ready to use
355-5754 9 ml x 25 tubes355-5756 90 ml x 6 bottles355-5791 2.3 L x 5 bagsDehydrated
356-4544 500 g
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
30°C72 hrs
Guide for Bio-Rad Products in Food Testing | | 3
ISO 16649-1 (04/2001)
Horizontal method for the enumeration
of -glucuronidase-positive E. coli
Part 1: Colony-count technique at 44°C using membranes and5-bromo-4-chloro-3-indolyl-
-D-glucuronide
TBX
Direct enumeration
Sample/
initial suspension
MMGA with membrane
ISO 16649-2 (04/2001)
Horizontal method for the enumeration
of -glucuronidase-positive E. coli
Part 2: Colony-count technique at44°C using 5-bromo-4-chloro-
3-indolyl- -D-glucuronide
Escherichia coli - ISO standards
Dilutions
Resuscitation
Direct enumeration
Sample/
initial suspension
Dilutions
TBX
Ordering Information
Catalog # Description
TBX/Agar
Ready to use
355-5309 100 ml x 6 bottlesDehydrated
356-4035 500 g
Peptone Water/Broth
Ready to use
355-4180 9 ml x 25 tubesDehydrated
356-4334 500 g
Tryptose Lauryl Sulfate/Broth
Dehydrated
356-4774 500 g
EC Medium/Broth
Dehydrated
356-4764 500 g
RAPID’E.coli 2/Agar
Ready to use
355-5297 200 ml x 6 bottles355-5299 100 ml x 6 bottlesDehydrated
356-4024 500 g
44°C18-24 hrs
Room t° 15 minand 37°C 4 hrs ± 1 hr
44°C18-24 hrs
* Please refer to ISO 7218: 2007
(2 X)*
4 | | Guide for Bio-Rad Products in Food Testing
ISO 7251 (07/2005)
General guidance for detection and
enumeration
of presumptive Escherichia coli
Most probable number technique
ISO/TS 16649-3 (09/2005)
Horizontal method for the enumeration
of -glucuronidase-positive E. coli
Part 3: Most probable numbertechnique using 5-bromo-4-chloro-3-
indolyl- -D-glucuronide
Enumeration of Escherichia coli
NF VALIDATION according to ISO 16140N° BRD 07/1-07/93 and BRD 07/7-12/04
AOAC-RI approved N° 050601 NordVal approved
All food stuffs
Sample/
initial suspension
RAPID’E.coli 2
Dilutions
Tryptose LaurylSulfate
EC Medium
Peptone Water
Indole test reading and calculation
Sample/
initial suspension
Single
concentration
Double
concentration
Routine Testing
37°C24-48 hrs ± 2 hrs
37°C24-48 hrs ± 2 hrs
44°C24-48 hrs± 2 hrs
44°C/37°C18-24 hrs
Escherichia coli Alternative method: RAPID’E.coli 2 Agar
Direct enumeration
of typical colonies*
* Enumeration of typical colonies
for E. coli at 44°C; for E. coli and
coliforms at 37°C
Sample/initial suspension
* For detection method only
(or)*
44°C48 hrs± 2 hrs
Each tube showing the presence of blue
colonies on TBX
should be considered positive
Calculate the most probable number
from the number of positive tubes
at each dilution
44°C20-24 hrs
37°C24 hrs ± 2 hrs
Add 10 ml
in 3 tubes of double
strength MMG
And subsequent dilution
Add 1 ml in 3 tubes
of single strength MMG
Subculture each tube presenting
yellow coloration on a dish of TBX
Guide for Bio-Rad Products in Food Testing | | 5
ISO 4831 (08/2006)
Microbiology of food and animal feeding stuffs
Horizontal method for the detection
and enumeration of coliforms
MPN technique
Sample
Tryptose Lauryl Sulfate
Coliforms - ISO standards
Dilutions
Ordering Information
Catalog # Description
BGBLB (Brilliant Green BileLactose Broth)/Broth
Ready to use
357-8024 10 ml x 25 tubes
VRBL (Violet Red BileLactose)/Agar
Ready to use
355-4799 100 ml x 6 bottlesDehydrated
356-4594 500 g
Tryptose Lauryl Sulfate/Broth
Dehydrated
356-4774 500 g
RAPID’E.coli 2/Agar
Ready to use
355-5297 200 ml x 6 bottles355-5299 100 ml x 6 bottlesDehydrated
356-4024 500 g
30°C or 37°C24-48 hrs ± 2 hrs
30°C or 37°C24 hrs ± 2 hrs
30-37°C24 hrs (+24 hrs)
Single
concentration
Double
concentration
Reading (gas +)
BGBLB
(or)*
* For detection method only
6 | | Guide for Bio-Rad Products in Food Testing
Routine Testing
ISO 4832 (02/2006)
Microbiology of food and animal
feeding stuffs
Horizontal method
for the enumeration of coliforms
Colony-count technique
ColiformsAlternative method: RAPID’E.coli 2 Agar
Enumeration of coliforms
NF VALIDATION according to ISO 16140N° BRD 07/8-12/04
AOAC-RI approved N° 050601NordVal approved
All food stuffs
Direct enumeration
of typical colonies*
Sample/
initial suspension
RAPID’E.coli 2
Dilutions
Enumeration
of characteristic colonies
and confirmation
for atypical colonies
Sample/
initial suspension
Dilutions
* Enumeration of typical colonies
for E. coli at 44°C; for E. coli and
coliforms at 37°C
VRBL
30-37°C24 hr ± 2 hrs
37°C18-24 hrs
Coliforms
• Diameter ≥ 0.5 mm
• Purple with or without
a red zone of precipitated bile
* Please refer to ISO 7218: 2007
(2 X)*
Guide for Bio-Rad Products in Food Testing | | 7
ISO 21528-1 (08/2004)
Microbiology of food and animal feeding stuffs
Horizontal methods for the detection
and enumeration of Enterobacteriaceae
Part 1: Detection and enumeration by MPN technique with pre-enrichment
VRBG
Oxidase Dextrose BCP
Yellow colour (+)
EE
Biochemical confirmation
Confirmation
Nutrient (2.8%)
Sample
Non selective
Pre-enrichment
Selective
enrichment
Buffered Peptone Water
Enterobacteriaceae
37°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
37°C18 hrs ± 2 hrs
Ordering Information
Catalog # Description
EE/Broth
Dehydrated
356-4794 500 g
VRBG (Violet Red Bile Glucose)/Agar
Ready to use
355-4239 100 ml x 6 bottlesDehydrated
356-4584 500 g
Nutrient (2.8%)/Agar
Ready to use
355-4478 8 ml x 25 tubesDehydrated
356-4484 500 g
Oxidase Test
355-3834 2 x vials of 50 oxidase disks
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
8 | | Guide for Bio-Rad Products in Food Testing
ISO 21528-2 (08/2004)
Microbiology of food and animal feeding
stuffs
Horizontal methods for the detection
and enumeration of Enterobacteriaceae
Part 2: Colony-count technique
Routine Testing
Reading
Dilutions
Oxidase (-) Dextrose BCP
yellow colour (+)
Biochemical confirmation
Confirmation
Nutrient (2.8%)
Sample/initial suspension
VRBG
37°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
(2 X)*
* Please refer to ISO 7218: 2007
ISO 15214 (08/1998)
Microbiology of food and animal feeding
stuffs
Horizontal method for enumeration
of Mesophilic Lactic Acid Bacteria
Colony-count technique at 30°C
Sample/initial suspension
Mesophilic Lactic Acid Bacteria
30°C72 hrs
Enumeration
MRS
(2 X)*
* Please refer to ISO 7218: 2007
Ordering Information
Catalog # Description
MRS (Man Rogosa and Sharp)/Agar
Dehydrated
356-4244 500 g
Guide for Bio-Rad Products in Food Testing | | 9
EN ISO 6579/A1 (02/2006)
Microbiology of food and animal feeding stuffs
Horizontal method for the detection of Salmonella spp.
Amendment 1 Appendix D: Detection of Salmonella spp. in animal faeces and in samplesfrom primary production stage
Salmonella - ISO standard
37°C ± 1°C24 hrs ± 3 hrs
37°C ± 1°C24 hrs ± 3 hrs
37°C ± 1°C24 hrs ± 3 hrs
41.5°C ± 1°C24 hrs ± 3 hrs
37°C ± 1°C18 hrs ± 2 hrs
RappaportVassiliadis Soya
Buffered Peptone Water
MKTTn
Appendix D Protocol
Biochemical and serological confirmation
TSI Agar and other test
Plating-out
Confirmation
Nutrient 2.1%
Sample
Non selective
pre-enrichment
Selective
enrichment
Confirmation
Selective
isolation
XLD RAPID’Salmonellaor XLT 4
or Kristensenor Hektoen
MSRV
41.5°C ± 1.5°C24 hrs ± 3 hrs(+24 hrs for negative)
10 | | Guide for Bio-Rad Products in Food Testing
Pathogen Testing
Ordering Information
Catalog # Description
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
RVS (Rappaport Vassiliadis Soya)/Broth
Ready to use
355-5773 10 ml x 25 tubesDehydrated
356-4324 500 g
MKTTN (Muller Kauffmann Tetrathionate Novobiocin)/Broth
Ready to use
355-6140 10 ml x 50 tubesDehydrated Base
356-4714 500 gNovobiocin supplement
356-4610 1 vial TSI (Triple Sugar Iron)/Agar
Dehydrated
356-4384 500 g
Nutrient (2.1%)/Agar
Dehydrated
356-4485 500 g
XLD (Xylose Lysine Desoxycholate)/Agar
Ready to use
354-1751 90 mm x 20 dishesDehydrated356-9124 500 g
Selenite-Cystine/Broth
Complete medium
355-5744 20 ml x 25 tubes
MSRV (Semi-Solid Rappaport Vassiliadis Medium)
Ready to use
355-6139 200 ml x 6 bottlesDehydrated
356-4325 500 g
XLT 4 (Xylose Lysine Tergitol 4)/Agar
Ready to use
356-3654 90 mm x 20 dishes
Brilliant Green (Kristensen)/Agar
Dehydrated
356-4464 500 g
Hektoen/Agar
Ready to use
355-4386 100 ml x 6 bottles356-3894 90 mm x 20 dishesDehydrated356-4284 500 g
RAPID’Salmonella/Agar
Ready to use
356-3961 90 mm x 20 dishes356-3962 90 mm x 100 dishesDehydrated
356-4705 500 g
Oxidase Test
355-3834 2 x vials of 50 oxidase disks
Antiserum SalmonellaOmnivalent OMNI-O (A60)
356-0781 3 ml dropper bottle
Salmonella Latex Kit
Detection of groups B to E and G
355-6710 75 tests
Salmonella spp. Latex Kit
355-6711 50 tests
Guide for Bio-Rad Products in Food Testing | | 11
Ordering Information
Catalog # Description
Antiserum SalmonellaOmnivalent OMNI-O (A60)
356-0781 3 ml dropper bottle
iQ-CheckTM Salmonella II Kit
357-8123 96 tests
ONPG Test
355-3822 Set of 50 disks
Oxidase Test
355-3834 2 x vials of 50 oxidase disks
RAPID’Salmonella/Agar
Ready to use
356-3961 90 mm x 20 dishes356-3962 90 mm x 100 dishesDehydrated
356-4705 500 g
RVS/Broth (Rappaport Vassiliadis Soy)
Ready to use
355-5773 10 ml x 25 tubesDehydrated
356-4324 500 g
Salmonella Latex Kit
Detection of groups B to E and G
355-6710 75 tests
Salmonella spp. Latex Kit
355-6711 50 tests
RAPID´Salmonella Capsules /Supplement
356-4709 100 x Quantity for 2.5 l 356-4710 100 x Quantity for 250 ml356-4712 1 x Quantity for 100 analyses
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
Salmonella - Alternative method: RAPID’Salmonella Agardouble enrichment protocol
37°C18 hrs± 2 hrs
41.5°C6-26 hrs
37°C24 hrs± 2 hrs
BufferedPeptone Water
RappaportVassiliadis Soya
RAPID’Salmonella
Sample
Plating-out
Reading & confirmation
Standard tests or Oxidase/OMNI-O/ONPG
Non selective
pre-enrichment
Selective
enrichment
Selective
isolation
Detection of Salmonella spp.
NF VALIDATION according to ISO 16140N° BRD 07/11-12/05
AOAC-RI approved N° 0507011
All food stuffs and feed
12 | | Guide for Bio-Rad Products in Food Testing
For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.1 - For AOAC-RI protocol, please contact your local Bio-Rad sales representative2 - 8-hr enrichment protocol available for raw meat (AOAC and AFNOR validated)
Pathogen Testing
Salmonella - Alternative method: RAPID’Salmonella Agarshort protocol
Salmonella - Alternative method: iQ-Check™ Salmonella II real-time PCR Kit
37°CFrom 10 hrs ± 2 hrsto 21 hrs ± 1 hr
41.5°C ± 1°C18 hrs± 2 hrs
37°C ± 1°C24 hrs± 2 hrs
Buffered Peptone Water+
Salmonella Capsule Supplement
RAPID’Salmonella
Sample
Plating-out
Reading & confirmation
Salmonella Latex or Oxidase/OMNI-O/ONPGor iQ-Check™ Salmonella
Buffered Peptone Water
Sample
Selective
enrichment
Selective
isolation
Detection of Salmonella spp.
Certification by NF VALIDATION according to ISO 16140 N° 07/11-12/05
Detection of Salmonella spp.
NF VALIDATION according to ISO 16140N° BRD 07/06-07/04
AOAC-RI approved N° 010803NordVal approved N° 038
All foods stuffs1,2, feed, and environment including
primary production
Bacterial Lysis & DNA Extraction
(Standard or Easy)
Real-time PCR amplification and detection
with iQ-Check™ Salmonella II
Automatic result analysis and interpretation
Confirmation of positive with RAPID’Salmonella
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchasedsolely in food testing, environmental testing, industrial microbiology and also for the purchaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
ater
e
Guide for Bio-Rad Products in Food Testing | | 13
�+/-
ISO/TS 22964:2006 Milk and milk products -
Detection of Enterobacter sakazakii
Cronobacter spp. - ISO standard
37°C18 hrs ± 2 hrs
44°C24 hrs ± 2 hrs
44°C24 hrs ± 2 hrs
25°C48 hrs
Streak onto RAPID’Sakazakii
Perform identification
tests on yellow colonies
Sample preparationWeigh z g of the test portion
in 9 x z ml BPW
Selective enrichment
Transfer 0.1 ml in 10 ml
of mLST/vancomycin medium
Transfer 1 to 5 colonies on
TSA
Ordering Information
Catalog # Description
RAPID’Sakazakii/Agar
Dehydrated
356-4976 500 g
Trypto-Casein-Soy (TSA)/Agar
Ready to use
356-3884 90 mm x 20 dishes 355-5947 200 ml x 6 bottles 355-5944 8 ml x 25 inclined tubes Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g iQ-CheckTM Cronobacter spp.
357-8137 96 tests
14 | | Guide for Bio-Rad Products in Food Testing
Pathogen Testing
RAPID’Sakazakii short protocol
NF VALIDATION according to ISO 16140N° BRD 07/22-05/12
Detection of Cronobacter spp. Infant Formulas and environment
NF VALIDATION pending
Cronobacter spp. - Alternative method: RAPID’Sakazakii Agarshort protocol
Cronobacter spp. - Alternative method:iQ-Check™ Cronobacter spp. real-time PCR Kit
37°C18 hrs ± 2 hrs
44°C24 hrs ± 2 hrs
25°C48-72 hrs
Streak onto RAPID’Sakazakii
Perform identification
tests on yellow colonies
Sample preparationWeigh z g of the test portion
in 9 x z ml BPW
Transfer 1 to 5 colonies on
TSA
Guide for Bio-Rad Products in Food Testing | | 15
Bacterial Lysis & DNA Extraction
(Standard or Easy)
Real-time PCR amplification and detection
with iQ-Check™ Cronobacter spp.
Automatic result analysis and interpretation
Confirmation of positive with RAPID’Sakazakii
37°C18 hrs ± 2 hrs
Buffered Peptone Water
Environmental
samples
37°C4 hrs ± 1 hr
37°C20 hrs ± 2 hrs
Buffered Peptone Water
Suppl. Buffered Peptone Water
Infant formulas
�+/-
EN ISO 11290-1/A1 (02/2005)
Horizontal method for the detection
and enumeration of Listeria monocytogenes
Part 1: Detection method
Reading andconfirmation
Isolation
Primary enrichment
Isolation Secondary enrichment
Sample
Fraser ½
Fraser 1(0.1 ml culture/10 ml)
Listeria spp.
- TSYEA agar
- Catalase +
- Gram
- Mobility
Listeriamonocytogenes
- TSYEB
- Hemolysis
- Carbohydrates
- Camp test
Agar Listeria accordingto Ottaviani and Agosti
Other solid selective medium
Listeria monocytogenes - ISO standards
30°C24 hrs ± 2 hrs
35°C or 37°C48 hrs ± 2 hrs
37°C ± 1°C24 hrs ± 3 hrs(+24 hrs ± 3 hrs)
30°C-35°C-37°C(dependingof the media)24-48 hrs ± 2 hrs
Reading andconfirmation
37°C ± 1°C24 hrs ± 3 hrs(+24 hrs ± 3 hrs)
30°C-35°C-37°C(dependingof the media)24-48 hrs ± 2 hrs
Agar Listeria according
to Ottaviani and Agosti
Other solid selective medium
16 | | Guide for Bio-Rad Products in Food Testing
EN ISO 11290-2/A1 (02/2005)
Horizontal method for the detection
and enumeration of Listeria monocytogenes
Part 2: Enumeration method
Listeria spp.
- TSYEA agar
- Catalase +
- Gram
- Mobility
Listeriamonocytogenes
- TSYEB
- Hemolysis
- Carbohydrates
- Camp test
Pathogen Testing
Enumeration and confirmation tests
Sample
Fraser 1/2 without LiCl
and selective supplement
or Buffered Peptone Water
Agar Listeria accordingto Ottaviani and Agosti
Dilution
20°C ± 2°C1 hr ± 5 min
37°C24 hrs ± 3 hrs(+ 24 hrs ± 3 hrs)
Ordering Information
Catalog # Description
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
Fraser ½/Broth
Complete medium
355-5797 225 ml x 6 bottles355-5788 2.3 L x 5 bagsBase
356-4604 500 gSupplement
356-4616 10 vials
Fraser 1/Broth
Complete medium
355-4569 10 ml x 25 tubesBase
356-4604 500 gSupplement
356-4615 10 vials
AL (Agar Listeria accordingto Ottaviani and Agosti)/Agar
Ready to use
356-3695 90 mm x 20 dishes356-3696 90 mm x 100 dishesBase
356-4043 500 gBase in vials (to be supplemented)
355-5200 237.5 ml x 6 bottlesSupplement 1
356-4201 100 capsules (1 capsule qsp 250 ml)356-4041 10 vialsSupplement 2
356-4042 10 vials
Palcam/Agar
Ready to use
356-3674 90 mm x 20 dishesBase
356-4754 500 gSupplement
356-4752 10 vials
Oxford/Agar
Ready to use
356-3664 90 mm x 20 dishes
Guide for Bio-Rad Products in Food Testing | | 17
Detection of Listeria monocytogenes
NF VALIDATION according to ISO 16140N° BRD 07/4-09/98
AOAC-RI approved N° 030406* NordVal approved
All food stuffs and environment
Listeria monocytogenes - Alternative method: RAPID’L.mono Agar
30°C24 hrs± 2 hrs
37°C24 hrs± 2 hrs
Isolation on
RAPID’L.mono
Reading and
confirmation tests
Fraser ½ Broth
Sample
Standard Tests
- TSYEB
- Hemolysis
- Carbohydrates
- Camp test
iQ-Check™
Listeria monocytogenes II
Spot on
Agar Listeria
according to
Ottaviani and
Agosti
Rhamnose Testor oror
Ordering Information
Catalog # Description
RAPID’L.mono/Agar
Ready to use
356-3693 90 mm x 100 dishes356-3694 90 mm x 20 dishes355-5294 Kit: 190 ml agar + 2 suppl.Base
356-4293 500 gSupplement 1
356-4294 10 vialsSupplement 2
356-4746 10 vials
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 gFraser ½/Broth
Complete medium
355-5797 225 ml x 6 bottles355-5788 2.3 L x 5 bagsBase
356-4604 500 gSupplement
356-4616 10 vialsAL (Agar Listeria accordingto Ottaviani and Agosti)/Agar
Ready to use
356-3695 90 mm x 20 dishes356-3696 90 mm x 100 dishesBase
356-4043 500 gBase in vials (to be supplemented)
355-5200 237.5 ml x 6 bottlesSupplement 1
356-4041 10 vials356-4201 100 capsules (1 capsule qsp 250 ml)Supplement 2
356-4042 10 vialsLSB (Listeria Special Broth)
Ready to use
355-5703 225 ml x 6 bottles355-5793 5 L x 2 bagsDehydrated
356-4703 500 g356-4753 5 kgiQ-CheckTM Listeria monocytogenes II Kit
357-8124 96 tests
Rhamnose Test
355-3669 28 tests
For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.*For AOAC-RI protocol, please contact your local Bio-Rad sales representative
18 | | Guide for Bio-Rad Products in Food Testing
Pathogen Testing
37°C24 hrs± 2 hrs
Isolation on
RAPID’L.mono
Direct
enumeration
Buffered Peptone Water
Sample
Enumeration of Listeria monocytogenes
NF VALIDATION according to ISO 16140N° BRD 07/05-09/01
All food stuffs and environment
20°C1 hr
Resuscitation
For a NF VALIDATION validated
enumeration method, in the event
of a positive result using
RAPID’L.mono for enumeration,
confirmation is not necessary when
the presence of Listeria monocytogenes
has been confirmed during detection.
Listeria monocytogenes - Alternative method: iQ-Check™ Listeria monocytogenes II real-time PCR Kit
30°C23 hrs ± 1 hr (1)
or 25 hrs ± 1 hr (2)
(1) Listeria Special Broth (LSB) or (2) Fraser ½ Broth
Sample
Detection of Listeria monocytogenes
NF VALIDATION according to ISO 16140N° BRD 07/10-04/05
AOAC-RI approved N° 010802NordVal approved N° 037
All food stuffs and environment
Bacterial Lysis & DNA Extraction
(Standard or Easy)
Real-time PCR amplification and detection with
iQ-Check™ Listeria monocytogenes II
Automatic result analysis and interpretation
Confirmation of positive with RAPID’L.mono
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchased solely in food testing, environmental testing, industrial microbiology and also for the pur-chaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contac-ting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Guide for Bio-Rad Products in Food Testing | | 19
�+/-
Reading and confirmation tests
Isolation on RAPID’Listeria spp.
Sample
Fraser ½ Broth
Standard
Tests
- Gram
- Catalase
Spot on
RAPID’L.mono+ Gram test
+ Catalase
Spot
on PalcamiQ-Check™
Listeria spp.
30°C24 hrs ± 2 hrs
37°C24 hrs ± 2 hrs
Ordering Information
Catalog # Description
RAPID’Listeria spp./Agar
Ready to use
356-3950 20 x 90 mm dishesBase
356-4744 500 gSupplement 1
356-4745 10 vials Supplement 2
356-4746 10 vials
Fraser ½/Broth
Ready to use
355-5797 225 ml x 6 bottles355-5788 2.3 L x 5 bagsBase
356-4604 500 gSupplement
356-4616 10 vials
Palcam/Agar
Ready to use
356-3674 90 mm x 20 dishesBase
356-4754 500 gSupplement
356-4752 10 vials
LSB (Listeria Special Broth)
Ready to use
355-5703 225 ml x 6 bottles355-5793 5 L x 2 bagsDehydrated
356-4703 500 g356-4753 5 kg
iQ-CheckTM Listeria spp. Kit
357-8113 96 tests
For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.
*For AOAC-RI protocol, please contact your local Bio-Rad sales representative
Listeria spp. - Alternative method:RAPID’Listeria spp. Agar
Detection of Listeria spp.
NF VALIDATION according to ISO 16140N° BRD 07/12-12/06
AOAC-RI approved N° 080701*All food stuffs and environment
or or or
20 | | Guide for Bio-Rad Products in Food Testing
Pathogen Testing
Listeria spp. - Alternative method:iQ-Check™ Listeria spp. real-time PCR Kit
Detection of Listeria spp.
NF VALIDATION according to ISO 16140N° BRD 07/13-05/07
AOAC-RI approved N° 090701*Health Canada Validation -MFLP-39
All food stuffs and environment
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchasedsolely in food testing, environmental testing, industrial microbiology and also for the purchaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
or
Detection of Listeria spp.
NF VALIDATION according to ISO 16140N° BRD 07/4-09/98
All food stuffs and environment
30°C24 hrs± 2 hrs
37°C24-48 hrs
Isolation on
RAPID’L.mono
Reading and confirmation tests
Fraser ½ Broth
Sample
Standard Tests
- TSYEA
- Gram
- Catalase
iQ-Check™
Listeria spp.
Listeria spp. - Alternative method: RAPID’L.mono Agar
Guide for Bio-Rad Products in Food Testing | | 21
30°C23 hrs ± 1 hr (1)
or 25 hrs ± 1 hr (2)
Listeria Special Broth (LSB)
Sample
Bacterial Lysis & DNA Extraction ((1) Standard or (2) Easy)
Real-time PCR amplification and detection with
iQ-Check™ Listeria spp.
Automatic result analysis and interpretation
Confirmation of positive with RAPID’Listeria spp. or RAPID’L.mono
�+/-
Ordering Information
Catalog # Description
RAPID’E.coli O157:H7/Agar
Base
356-4748 100 g
mTSB/Broth
Ready to use
(containing novobiocin)
355-5426 225 ml x 6 bottlesBase
356-4426 500 g
Novobiocin
Dehydrated
356-4610 1 g
Buffered Peptone Water/Broth
Ready to use
355-4179 225 ml x 6 bottles355-5789 2.3 L x 5 bags355-5790 5 L x 2 bagsDehydrated
356-4684 500 g
iQ-CheckTM E.coli O157:H7 Kit
357-8114 96 tests
iQ-CheckTM Campylobacter Kit
357-8135 96 tests
For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.
*For AOAC-RI protocol, please contact your local Bio-Rad sales representative
Escherichia coli O157:H7 - Alternative method: RAPID’E.coli O157:H7 Agar
41.5°C16-24 hrs
mTSB + Novobiocin
Immuno-magnetic-separation
Sample
37°C24 hrs ± 2 hrs
Reading and confirmation tests
Standard tests or Latex or
iQ-Check™ E.coli O157:H7
Isolation on RAPID’E.coli O157:H7
Detection of Escherichia coli O157:H7
NF VALIDATION according to ISO 16140N° BRD 07/14-09/07
AOAC-RI approved N° 060701*All food stuffs and environment
RAPID’E.coli O157:H7 can also be used as a second medium
in the ISO 16654 standard
22 | | Guide for Bio-Rad Products in Food Testing
Pathogen Testing
Escherichia coli O157:H7 - Alternative method: iQ-Check™ E.coli O157:H7 real-time PCR Kit
Campylobacter spp. - Alternative method: iQ-Check™ Campylobacter real-time PCR Kit
41.5°C8-24 hrs
41.5°C20 hrs
37°C 4 hrs
42°C 24 hrs
Buffered Peptone Water
Suppl. Bolton Broth 2X BF-BEB
Sample Food Sample Carcass rinse or sponge
Detection of Escherichia coli O157:H7
NF VALIDATION according to ISO 16140 N° BRD 07/15-06/08 Raw beef
AOAC-RI approved N° 020801*Raw ground beef, fresh spinash, apple cider
Detection of Campylobacter jejuni, Campylobacter coli
and Campylobacter lari
AOAC-RI approved N° 031209*
Food samples, carcass rinses or sponges and feces samples
Bacterial Lysis &
Easy DNA Extraction
Bacterial Lysis and DNA Extraction
(Easy)
Real-time PCR amplification and detection
with iQ-Check™ E.coli O157:H7
Real-time PCR amplification and detection
with iQ-Check™ Campylobacter
Result analysis and interpretation Result analysis and interpretation
Confirmation of posiitve with RAPID’E.coli O157:H7
Purchase of iQ-Check Kits includes an immunity from suit under patents specified in the product insert to use only the amount purchasedsolely in food testing, environmental testing, industrial microbiology and also for the purchaser’s own internal research. No other patent rights (such as 5’ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Guide for Bio-Rad Products in Food Testing | | 23
+
�+/- �+/-
NF EN ISO 6888-1/A1 (01/2004)
Horizontal method for the enumeration of
coagulase-positive Staphylococci
(Staphylococcus aureus and other species)
Technique including confirmation of colonies
Staphylococcus aureus - ISO standards
35°C or 37°C24 hrs ± 2 hrs(+ 24 hrs ± 2 hrs)
35°C or 37°C24 hrs ± 2 hrs
35°C or 37°C4-6 hrs or 24 hrs
Confirmation
Brain-Heart Infusion
Rabbit Plasma
Coagulation reading
Sample/initial suspensionand following dilution
Enumeration
Typical Staphylococci
Grey to black colonies with a clear halo around
and a white opaque zone
Ordering Information
Catalog # Description
Baird-Parker/Agar
Complete medium
356-3991 90 mm x 20 dishesBase
356-4814 500 g
Baird-Parker for RPF/Agar
Complete medium
357-8618 90 ml x 6 bottles + 6 vials of suppl.356-3996 90 mm x 20 dishesBase
356-4814 500 g
Additives
Egg yolk with potassium tellurite
355-4201 5 ml x 1 vial355-4205 25 ml x 1 bottleSulfamethazine solution (0.2%)
356-2682 2.5 ml x 1 vial
PastorexTM Staph Plus Latex
355-6356 50 tests355-6353 5 x 50 tests
RPF Supplement
356-4618 10 vials
BHI (Brain-Heart Infusion)/Broth
Ready to use
355-3664 10 ml x 25 tubesDehydrated
356-4014 500 g
Rabbit Plasma
Freeze-dried
355-6352 Pack for 20 reactionsSpecial broth
355-3544 9 ml x 25 tubes
RAPID’Staph/Agar
Ready to use
356-3960 90 mm x 20 dishesBase
356-4704 500 g
Baird-Parker
(2 X)*
* Please refer to ISO 7218: 2007
24 | | Guide for Bio-Rad Products in Food Testing
NF EN ISO 6888-2/A1 (12/2003)
Horizontal method for the enumeration
of coagulase-positive Staphylococci
(Staphylococcus aureus and species)
Technique using Rabbit plasma Fibrinogen agar medium
NF EN ISO 6888-3 (06/2003)
Horizontal method for the enumeration
of coagulase-positive Staphylococci
(Staphylococcus aureus and species)
Detection and MPN techniquefor low numbers
35°C or 37°C18-24 hrsand 48 hrs
37°C24 hrs ± 2 hrs (+24 hrs ± 2 hrs)
37°C24-48 hrs
37°C4-6 hrsor 24 hrs
37°C24 hrs
37°C24-48 hrs
Direct enumeration of typical
coagulase-positive staphylococci
Sample/initial suspensionand following dilution
Rabbit Plasma
Confirmation of
typical colonies
on BHI
Reading
Direct
enumeration
of typical
coagulase-
positive colonies
Sample/initial suspension
Giolitti and CantoniBroth
Simple concentration
Baird-Parker Baird-Parkerfor RPF
+ RPF sup.
37°C24 hrs ± 2 hrs
RAPID’Staph
Sample/initial suspension
Enumeration and Confirmation
Staphylococcus aureusAlternative method:RAPID’Staph Agar
Baird-Parkerfor RPF
+ RPF sup.
or
Gioletti and CantoniBroth
Double concentration(or)*
* For detection method only
Enumeration of Coagulase-positive
Staphylococci
NF VALIDATION according to ISO 16140N° BRD 07/9-02/05
AOAC-RI approved N° 080602*All food stuffs, feed and environment
Pathogen Testing
(2 X)*
* Please refer to ISO 7218: 2007
PastorexTM
Staph Plus
Spot on
Baird-Parker
for RPFor
* For AOAC-RI protocol, please contact your local Bio-Rad sales representative
Guide for Bio-Rad Products in Food Testing | | 25
ISO 15213 (05/2003)
Microbiology of food and
animal feeding stuffs
Horizontal method for
the enumeration of sulfite-reducing
bacteria growing
under anaerobic conditions
Reading
Food sample/initial suspension
Dilution
Confirmation
NF EN ISO 7937 (02/2005)
Horizontal method for the enumeration
of Clostridium perfringens
Colony-count technique
Sulfite-reducing bacteria Clostridium perfringens
Thermophilous
bacteria
suspicion
50°C 24-48 hrs
Anaerobes
condition
LactoseSulfite
Ordering Information
Catalog # Description
TSC without D-Cycloserine/Agar - Base Medium
Ready to use
355-4419 100 ml x 6 bottlesBase
356-9644 500 g
Lactose Sulfite/Broth
Base
356-4914 100 g
Thioglycolate + Resazurin/Broth
Ready to use
356-3613 10 ml x 25 tubesDehydrated
356-4084 500 g
Sheep Blood/Agar
Base
356-4674 500 gReady to use
356-3784 90 mm x 20 dishesSheep Blood Supplement
355-6652 50 ml vial
37°C24-48 hrsanaerobescondition
Heat treatment 20 min 75°C
37°C20 hrs ± 2 hrs
37°C18-24 hrs
37°C18-24 hrs
46°C24 hrs
Enumeration
of typical black colonies
Confirmation
of 5 isolated colonies with
Thioglycolate + Resazurin
Food sample/initial suspension
MobilityNitrate
LactoseGelatine
Clostridiumperfringens
(yellow/gas+)
Clostridiumperfringens
(red coloration/
gas+)
Clostridiumperfringens
(black precipi-
tate/gas+)
Anaerobes
condition
TSC
TSC (add D-Cycloserine)
(2 X)*
* Please refer to ISO 7218: 2007
(2 X)*
* Please refer to ISO 7218: 2007
26 | | Guide for Bio-Rad Products in Food Testing
Bacillus cereus
NF EN ISO 7932 (07/2005)
General guidance for the enumeration
of Bacillus cereus
Colony-count technique at 30°C
Confirmation test
on 5 well isolated colonies
Food sample/initial suspension
Pathogen Testing
30°C24 hrs ± 2 hrs
30°C18-24 hrs(+24 hrs)
Sheep Blood/Agar
Reading and interpretation
Ordering Information
Catalog # Description
Mossel/Agar (Mannitol Egg Yolk Polymixin)
Dehydrated
356-9604 500 g
XLD (Xylose Lysine Desoxycholate)/Agar
Ready to use
354-1751 90 mm x 20 dishesDehydrated
356-9124 500 g
Mac Conkey + Crystal Violet/Agar
Ready to use
356-3617 90 mm x 20 dishesDehydrated
356-9084 500 g
Hektoen/Agar
Ready to use
355-4386 100 ml x 6 bottles356-3894 90 mm x 20 dishesDehydrated
356-4284 500 g
Nutrient (2.1%)/Agar
Dehydrated
356-4485 500 g
Novobiocin
Dehydrated
356-4610 1 g
Mossel (MYP)
Shigella
NF EN ISO 21567 (03/2005)
Horizontal method
for the detection of Shigella spp.
37°C20-24 hrs(+48 hrs)
37°C20-24 hrs
41°C16-20 hrs
Plating-out
Shigella Broth + NovobiocinPH 7 ± 0.2
Spot on typical colonies on
Nutrient (2.1%)
Food sample/initial suspension
Biochemical and serologicalconfirmation
MacConkey
XLD Hektoen
(2 X)*
* Please refer to ISO 7218: 2007
Guide for Bio-Rad Products in Food Testing | | 27
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Website www.bio-rad.com/diagnostics U.S. 1-800-2BIO-RAD Australia 61-2-9914-2800 Austria 43-1-877-8901 Belgium 32-9-385-5511 Brazil 5521-3237-9400
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