a metabolomic approach to the identification of cancer biomarkers

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A Metabolomic Approach to the Identification of Cancer Biomarkers. Clive Baveghems Bioanalytical Chemistry Spring 2011. Outline. What is Metabolomics? Metabolomic screening for the identification of cancer biomarkers Metabolomic screening for the identification of prostate cancer biomarkers. - PowerPoint PPT Presentation

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A Metabolomic Approach to the Identification of Cancer

BiomarkersClive Baveghems

Bioanalytical ChemistrySpring 2011

Outline

• What is Metabolomics?• Metabolomic screening for the

identification of cancer biomarkers• Metabolomic screening for the

identification of prostate cancer biomarkers

Introduction to Metabolomics

Sawyer, J. Surg. Oncol. 2010; 21831: 2

Yearly increase in Metabolomics publications

Ong, Trends in Analytical Chemistry 2010; 29(3):270

Note: 2010 total ~ 750

Factors influencing the Metabolome

Sawyer, J. Surg. Oncol. 2010; 21831: 2

Outline

• What is Metabolomics?√• Metabolomic screening for the

identification of cancer biomarkers• Metabolomic screening for the

identification of prostate cancer biomarkers

Biomarker Identification

Sawyer, J. Surg. Oncol. 2010; 21831: 4

Cancer biomarkers identified so far

Sawyer, J. Surg. Oncol. 2010; 21831: 5

Key Metabolites implicated so far

Sawyer, J. Surg. Oncol. 2010; 21831: 6

Outline

• What is Metabolomics?√• Metabolomic screening for the

identification of cancer biomarkerså Metabolomic screening for the

identification of prostate cancer biomarkers

Prostate Cancer

• From 2003-2007 median age for diagnosis was 67

• The age-adjusted incidence rate was 156.9/100, 000 men per year

• The median age at death was 80• The age-adjusted death rate was

24.7/100, 000 men per year

http://seer.cancer.gov/statfacts/html/prost.html

Results from LC and GC-MS

Chinnaiyan, Nature 2009; 457(12): 911

Sarcosine identified as a biomarker for prostate cancer

Chinnaiyan, Nature 2009; 457(12): 913

Is sarcosine a biomarker for prostate cancer?

Jung, Journal of Urology 2011; 185: 708

GC-MS data

Summary

• Metabolomics has emerged as a fast and efficient method for the identification of a small set of candidate biomarkers from a large eclectic data set

• LC/MS, GC/MS, and NMR are important metabolomic technologies

• Future studies will further explain sarcosine’s role in Prostate Cancer

References• Metabolomics and Surgical Oncology: Potential Role for Small

Molecule Biomarkers Sawyer, J. Surg. Oncol. 2010; 21831: 1-9

• Metabolomic profiles delineate potential role for sarcosine in

prostate cancer progression Chinnaiyan, Nature 2009; 457(12): 910-915

• Sarcosine in Prostate Cancer Tissue is Not a Differential Metabolite for Prostate Cancer Progressiveness and Biochemical Progression Jung, Journal of Urology 2011; 185: 706-711

• Experiment-originated variations, and multi-peak and muti-origination phenomena in derivatization-based GC-MS metabolomics Ong, Trends in Analytical Chemistry 2010; 29(3):269-280

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