a metabolomic approach to the identification of cancer biomarkers
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A Metabolomic Approach to the Identification of Cancer
BiomarkersClive Baveghems
Bioanalytical ChemistrySpring 2011
Outline
• What is Metabolomics?• Metabolomic screening for the
identification of cancer biomarkers• Metabolomic screening for the
identification of prostate cancer biomarkers
Introduction to Metabolomics
Sawyer, J. Surg. Oncol. 2010; 21831: 2
Yearly increase in Metabolomics publications
Ong, Trends in Analytical Chemistry 2010; 29(3):270
Note: 2010 total ~ 750
Factors influencing the Metabolome
Sawyer, J. Surg. Oncol. 2010; 21831: 2
Outline
• What is Metabolomics?√• Metabolomic screening for the
identification of cancer biomarkers• Metabolomic screening for the
identification of prostate cancer biomarkers
Biomarker Identification
Sawyer, J. Surg. Oncol. 2010; 21831: 4
Cancer biomarkers identified so far
Sawyer, J. Surg. Oncol. 2010; 21831: 5
Key Metabolites implicated so far
Sawyer, J. Surg. Oncol. 2010; 21831: 6
Outline
• What is Metabolomics?√• Metabolomic screening for the
identification of cancer biomarkerså Metabolomic screening for the
identification of prostate cancer biomarkers
Prostate Cancer
• From 2003-2007 median age for diagnosis was 67
• The age-adjusted incidence rate was 156.9/100, 000 men per year
• The median age at death was 80• The age-adjusted death rate was
24.7/100, 000 men per year
http://seer.cancer.gov/statfacts/html/prost.html
Results from LC and GC-MS
Chinnaiyan, Nature 2009; 457(12): 911
Sarcosine identified as a biomarker for prostate cancer
Chinnaiyan, Nature 2009; 457(12): 913
Is sarcosine a biomarker for prostate cancer?
Jung, Journal of Urology 2011; 185: 708
GC-MS data
Summary
• Metabolomics has emerged as a fast and efficient method for the identification of a small set of candidate biomarkers from a large eclectic data set
• LC/MS, GC/MS, and NMR are important metabolomic technologies
• Future studies will further explain sarcosine’s role in Prostate Cancer
References• Metabolomics and Surgical Oncology: Potential Role for Small
Molecule Biomarkers Sawyer, J. Surg. Oncol. 2010; 21831: 1-9
• Metabolomic profiles delineate potential role for sarcosine in
prostate cancer progression Chinnaiyan, Nature 2009; 457(12): 910-915
• Sarcosine in Prostate Cancer Tissue is Not a Differential Metabolite for Prostate Cancer Progressiveness and Biochemical Progression Jung, Journal of Urology 2011; 185: 706-711
• Experiment-originated variations, and multi-peak and muti-origination phenomena in derivatization-based GC-MS metabolomics Ong, Trends in Analytical Chemistry 2010; 29(3):269-280
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