apoptosis. necrosis and apoptosis apoptosis morphological
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Apoptosis
Necrosis and Apoptosis
Apoptosis Morphological
Morphological
Necrosis Biochemical
Apoptosis Biochemical
Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin D (Lane 2). DNA Markers (M) were electrophoresed as a base pair reference.
DNA ladder
nucleosome
FACS DNA
Anexin V
Anexin V
Necrosis physical
Apoptosis physical
Physical significance
3. Detection of Apoptosis
• Morphological
• DNA electrophoresis
• DNA 断裂的原位末端标记 TUNEL 测定法， Terminal deoxynucleotid
yl transferase(TdT)-mediated dUTP end labeling ，末端脱氧核苷酸移换酶介导的 dUTP缺口末端标记测定法
• Flowmetery
3.Regulation of Apoptosis
⑴ Genes for proliferation
c-myc
ras
⑵ For survival
bcl-2
c-kit
⑶ proliferation inhibitors
p53
Rb
⑷ death promotors
Bax
ICE
4.p53 and apoptosis
2002 Press Release
Laureates
Ap-overview
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