august, 2011 · post-laser co2 laser, fraxel laser, etc. post-ipl post-mesotherapy, micro needle...

August, 2011

K O R E A N D E R M A T O L O G I S T ’ S C H O I C E - P O S T P R O C E D U R E S O L U T I O N

PPS (Post Procedure Solution)

Cosmetic surgery

Remove epidermis, collagen contraction

Cosmetic surgery (Laser, Peeling, Micro needle therapy, etc.)

Wound healing Process

Skin Regeneration (New collagen)

*Produce Partial thickness injury to the skin insult to the skin wound healing process

Injury

Inflammation

ECM accumulation

New tissue formation

Complete wound healing process

PPS (Post Procedure Solution)

Transition of post procedure product

1ST generation

(Antibiotics)

2nd generation

(Skin rejuvenation)

3rd generation

(PPS)

Action

Anti-microbial

& anti-inflammation

Stimulate tissue formation

Anti-microbial & anti-inflammation

+ Stimulate tissue formation

+ Skin protection

Indication

Wound infection, Infectious disease (folliculitis, etc.)

Cicatrize skin (ulcerous injury, traumatic injury, Etc.)

Post cosmetic procedure

PPS act on every step of wound healing process.

Because of the function of Centella asiatica, it greatly affect on new tissue formation.

PPS (Post Procedure Solution)

PPS & Wound Healing Process

Coagulation

& early inflammation

12-24 hour after

Late inflammation & Granulation tissue formation

3-7 day after

Proliferation & Remodeling

1-2 week after

[Wound healing process]

[PPS]

•Anti-microbial

•Anti-inflammation

•Prevent prolonging inflammatory phase

•Stimulate granulation tissue formation

•Promote angiogenesis

•Promote re-epithelialization

•Stimulate collagen type 1&3 synthesis

•Prevent hypertrophic & Keloid scar

•Stimulate ECM accumulation

PPS (Post Procedure Solution)

Centella asiatica

A plant which grows spontaneously

Wound healing by traditional medicine in asiatic countries.

Used for more than 3000years in traditional Chinese, Malaysian and Indian medicine for wound healing, as tonic or slimming agent.

Treatment of wounds and ulcers as drug in Europe

PPS (Post Procedure Solution)

Functions of Centella asiatica

Anti-microbial

Anti-inflammation

Anti-oxidant

Promote angiogenesis

Stimulate collagen type 1 & 3 synthesis

TGF β Receptor 1 kinase activation

Increase ECM production

PPS (Post Procedure Solution)

Silicone based Technology

•stabilization of active ingredient

•Skin protection from detrimental environment

•Inhibition of bacteria multiplication

•Safe on skin

•Non-comedogenic

PPS (Post Procedure Solution)

Comparison of two Ingredients for Skin protection

Petrolatum High molecular silicone

Action Mechanism Prevention of water loss by Making film on the surface of skin

Skin protection film

Very oily and glossy.

Block the pore (Obstruct skin breathing)

Comedogenic

Very smooth and silky

Unblock pore Skin breathing

Non-comedogenic

Anti-bacterial No reference Anti-bacterial

Physicochemical characteristic

Extraction and purification from petroleum

Unstable in high temperature

(Melt under 38 ℃)

Derived from silica

Stable for heat and oxidation

PPS (Post Procedure Solution)

Additional Ingredients

β-Glucan – Control immune system

Biosaccharide gum-1 – Anti-allergic function, Increase cutaneous elasticity

Sodium hyaluronate – Persistent moisturizing, stimulates the migration and mitosis of fibroblasts and epithelial cells.

Glycyrrhizin – Anti-inflammatory action

Betaine(Botanical glycine) -One of the most important component of collagen

ALOE vera gel - Support formation of granulation tissue

PPS (Post Procedure Solution)

Indications

Post-Laser

CO2 Laser, Fraxel Laser, etc.

Post-IPL

Post-Mesotherapy, Micro needle therapy

Post-Peeling

Micro dermabrasion

PPS (Post Procedure Solution)

PPS; Post Procedure Solution

Volume: 15ml, 50ml, 150ml

Active ingredients: Centella asiatica, Glycyrrhizin, Beta-Glucan, Arbutin, Aloe vera, Hyaluronic acid, Betaine, Biosaccharide gum-1

Action: Wound healing process promoting

Directions: Apply immediately post-procedure. Use liberally whenever treated area feels or looks dry. (every 2~3 hours for the first day and then every 4~6 hours for the next 7~10days)

Clinical photos

Case 1

Immediately after Phenol peel

1 day

2 days

3 days

8 days

Remove eschar

9 days

Case 2

0 day

After Phenol peel

1 day 2 days 9 days

Burn of third degree

12 days after

16DAYS AFTER

23DAYS AFTER

16DAYS AFTER

23DAYS AFTER

16DAYS AFTER

23DAYS AFTER

Clinical study of PPS as post-cosmetic

procedure care (wound dressing)

OhKim’s dermatology

M.D.Seung joon Jung

M.D., PhD. Hongjik Kim

Skin & Laser

Case 1.: 62Year’s old Nevus (Pigmentid), Seborrheic Keratosis, Lentigo ND YAG GREEN, IPL

0day (07.02.27) 1 day after (07.02.28) 3days after (07.03.02)

4days after (07.03.03) 11days after (07.03.10)

Case2. 62 years old, woman Nevus (Pigmentid), Co2 laser

0day 1 day after 4 days after

17 days after 14 days after

Case3. 40 years old, woman Nevus (Pigmentid), Erbium+Co2+IPL

0day 1 day after 4 days after

17 days after 14 days after

Case4. 40 years old, woman Nevus (Pigmentid) on the nose Erbium+Co2+IPL

1 day after 3 days after

6 days after

4 days after

Case5. 40 years old, woman Nevus (Pigmentid) on the nose Erbium+Co2+IPL

1 day after 3 days after

6 days after

4 days after

PPS (Post Procedure Solution)

Centella asiatica; Medical uses

•Treatment of wounds, burns, and ulcerous skin ailments,

and prevention of keloid and hypertrophic scars

•Extracts of the plant have been employed to treat

second- and third-degree burns.

•Extracts have been used topically to accelerate healing,

particularly in cases of chronic postsurgical and post-

trauma wounds.

Ref.) WHO monographs on selected medicinal plants, World Health Organization Geneva, 1999

References

PPS (Post Procedure Solution)

Drugs; TECA Ref.) Product information of Madecassol, International

development and manufacturing (Vidal 1995)

PROPERTIES:

Madecassol has a stimulating action on the biosynthesis of collagen by the fibroblasts of human venous walls and dermis in cellular culture.

It may therefore contribute to an improved trophicity of the connective tissue.

INDICATIONS:

Local treatment of leg ulcers from venous origin.

Fibrous or hypertrophic scars.

Surgical wounds and light burns.

DRUGS:

MDECASSOL® poudre et et onguent

MADECASSOL ® NEOMYCINE HYDROCORTISONE

MADECASSOL ® TULGRAS ®

References

PPS (Post Procedure Solution)

TECA and its separated components on the secretion of collagen

25㎍.㎖-1 for TECA

(AA) 7.5 ㎍.㎖-1 for asiatic acid

(MA) madecassic acid

(AO) 10 for asiaticoside

Ref.) Stimulation of collagen synthesis in fibroblast cultures by a triterpene extracted from centella asiatica. Connective Tissue Research, 1990. Vol. 24, pp. 107-120

References

PPS (Post Procedure Solution)

ECM accumulation

Ref.) Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat experimental wounds,

Eur J Dermatol; 1999; 9: 289-96

TECA-injected wound chambers were characterized by

increased dry weight, DNA, total protein, collagen and

uronic acid contents.

Peptidic hydroproline was also increased, showing an

increased remodeling of he collagen matrix in the wound.

In addition to collagen, it was also able to stimulate

glycosaminoglycan synthesis.

Hydroproline역시 증가되었으며 이는 상처부위에서 콜라겐 매트릭스의 재생이 증가되었음을 보여주는 것이다. 그것은 또한 GAGs의 생합성도 촉진하였다.

References

PPS (Post Procedure Solution)

Collagen 1 synthesis

Ref.) Influence of Asiatic Acid, Madecassic Acid, and Asiaticoside on Human Collagen 1 Synthesis. Planta Med. 60 (1994)

Asiatic acid, madecassic acid, and asiaticoside, terpenoids with an ursane skeletone, were tested separately and in combination on skin human fibroblast collagen 1 synthesis in vitro. In the absence of ascorbic acid, the mixture as well as each individual component stimulated collagen 1 synthesis to similar extent.

References

PPS (Post Procedure Solution)

Collagen type I & III collagen synthesis

Normal adult dermal fibroblast cultures were established using the explant method from a skin (lifting) sample obtained from a 50 year-old woman. Fibroblasts were grown to confluence in supplemented E 199 medium and after 24 hours of growth, products were added in serum free medium containing 0.15 mM sodium ascorbate. The media were then collected and type I and III collagen secretion levels determined. Kinetics of type I and III collagen secretion led to determine the effects to asiaticoside and madecassoside after 48 hours for collagen I secretion and 72 hours for collagen III. Asiaticoside and madecassoside, were shown to stimulate collagen secretion. Type I secreted collagen (for 10(4) fibroblasts per 48 hours) was increased for 25- 30% with asiaticoside and madecassoside. Interestingly, only Madecassoside was able to increase significantly collagen III secretion.

Ref.) Bonte F, Dumas M, Comparative activity of asiaticoside and madecassoside on type 1 and III collagen synthesis by cultured human fibroblast, Ann Pharm Fr. 1995; 53(1)38-42

References

PPS (Post Procedure Solution)

Anti-inflammation and Anti-microbial effect

Ref.) Loiseau A.*, Theron E.*, Buche P.*, Girard P** Evidencing the anti-microbial properties of Centella asiatica Laboratoires Roche Nicholas SA Division Serdex

More recent in vitro development revealed new properties for each

of these molecules and highlighted that Centella asiatica heterosides

possess an action of factors controlling the regulation of inflammation.

An aisaticoside extract significantly reduced IL 1 α liberation.

In the present study, the antibacterial activity of a purified triterpene

acid extract of Centella asiatica was demonstrated in vitro on

Staphylocuccus aureus, Streptococcus (group B) Propionbacterium

ACNES and Neisseria gonorrhoeae, confirming the specific properties

of another family of molecules (genins) from Centella asiatica.

References

PPS (Post Procedure Solution)

Antioxidant Effect in healing wounds

Asiaticoside derived from the plant Centella asiatica is known to possess good wound healing activity. Enhanced healing activity has been attributed to increased collagen formation and angiogenesis. Since antioxidants have been reported to play a significant role in the wound healing process we studied the effect of asiaticoside on the levels of certain antioxidants in the wound so as to explore the possible involvement of such a mechanism in the asiaticoside induced wound healing. Asiaticoside application (0.2%, topical) twice daily for 7 days to excision-type cutaneous wounds in rats led to increased enzymatic and non-enzymatic antioxidants, namely superoxide dismutase (35%), catalase (67%), glutathione peroxidase (49%), vitamin E (77%) and ascorbic acid (36%) in newly formed tissues. It also resulted in a several fold decrease in lipid peroxide levels (69%) as measured in terms of thiobarbituric acid reactive substance. However, continued application for 14 days showed no significant difference in these antioxidants compared with their values in vehicle treated wound tissue. It appears from the present study that asiaticosides enhanced induction of antioxidant levels at an initial stage of healing which may be an important contributory factor in the healing properties of this substance.

Ref.) Shukla A. Rasik AM, Asiaticoside-induced elevation of antioxidant levels in healing wounds. Pharmacology department, Central Drug Research Institute, Lucknow India.

References

PPS (Post Procedure Solution)

Promotes Angiogenesis

It promoted angiogenesis in the chick chorioallantoic membrane model at 40㎍/disk concentration.

Ref.) A. Shukla, A.M. Rasik, In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica, Journal of Ethnopharmacology, Volume 65, Issue 1: pp.1-11, April, 1999

40㎍/disk 농도에서 뺨의 융모막모델에서 신혈관생성을 개선한다.

References

PPS (Post Procedure Solution)

Wound healing effect in delayed healing models

Ref.) A. Shukla, A.M. Rasik, In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica, Journal of Ethnopharmacology, Volume 65, Issue 1: pp.1-11, April, 1999

These result indicate that asiaticoside exhibits significant wound healing activity in normal as well as delayed healing models and is the main active constituent of Centella asiatica.

References