•what is it? - ethanol lopez.pdfcellulosic ethanol production • great fiber source already on...

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• What is it?

–Ear of Teosinite

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The amazing corn kernel just became more amazing!

Introducing Enogen Corn®

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Corn has Come a Long

Way Over the Course of

the Last 7000 Years

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That Little Kernel – is full of little Goodies!

Great Energy Source ~7500 btu’s/lb

3 Major Components

• Pericarp ~ 6% of the Kernel – But 50% of Fiber in the Kernel

• Endosperm ~ 80% of Kernel– Starch

– Source of energy for the germinating seed

– 2 Components

• Floury Endosprem: is the softer more accessible starch

• Horneous Endosperm: harder to access starch as granules are embedded in a dense protein matrix

• Germ 12% of the Kernel – Comprised 25% fat (corn oil)

– Essential Amino Acids

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The Amazing Kernel

Is a great feedstock full of all those goodies, and it is;

• Energy Dense

• Easy to ship

• Stores Well

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Amazing Kernel Continues to Evolve

• Over the Course of the last 30 years Corn Yields have almost doubled

• Improved Genetics

• Molecular Breeding

• Biotechnology (Traits)

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Next Step in the Evolution of that Amazing Kernel

Enogen Corn

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Robust, optimized alpha-amylase enzyme delivered directly in the corn grain required by every ethanol plantC

What is Enogen “corn-expressed amylase”?

Broad Temperature Range and pH Tolerance

alpha-amylase

Features

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Enogen is a Thermostable Alpha

• Optimal Operating Temp 194 F or 90 C

• Denaturing Begins to Occur at 238 F or 115 C after 8 minutes

• No issues with Jet Cooker

• No Need for Secondary Alpha Dose

Syngenta Public Information

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• +90% Activity from 4.2 – 6.0 pH

• No pH Adjustment Required in Cook

• Eliminating NH3 addition to Cook

• Reducing Sulfuric Additions for pH Adjustment

• Broad pH Tolerance

Enogen Corn has Broad Range pH Tolerance

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Enzyme’s Unique Mode of Action

• Enogen’s Mode of Action: exhibits a random attack which quickly reduces chain length.

• This rapid reduction in the amylose chain length leads to a dramatic break in viscosity.

• Conventional alphas exhibit a multiple attack which generates smaller reducing sugars without significantly reducing chain length.

• The difference in enzyme mechanics

cannot be overcome by increased

dosage.

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• Improved Starch Hydrolysis

What does the Unique Mode of Action Mean?

0.0

1.0

2.0

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5.0

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

% R

esi

du

al

Sta

rch

Residual Starch

Res Starch with Glucose

Res Starch w/o Sugars

Enogen Trial

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What does the Unique Mode of Action Mean?

Increase in Soluble Sugars

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5

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/14

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/14

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UNIT 2 CONV. II

4.2

Total Sugars Liquefaction

DP4+

DP3

DP2

DP1

Enogen Trial

Competitors Liq Alpha

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Enogen Corn

Reduced Starch + Increase Soluble Sugars = YIELD

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Enogen Viscosity Break

Viscosity Break is Significant

~ 50% Decrease in Mash Viscosity in Cook

~ 50% Decrease in variability range in Mash viscosity

Increased Thermal Conductivity of Mash

Efficiency Gains in Heat Exchangers

Reduction in Pumping amps are immediate

Processing FlexibilityDe-water PlantIncreased throughput Increased YieldOr Both

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Slurry 36.5% Solids

As of July 25, 2013

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Fermentation with Enogen Corn• DE’s coming out of Liq will

increase to ~20

• Need to Remember we are creating new/additional fermentable sugars: 10% increase on average

• These Sugars are Fermentable

• GA Dosing and Dosing Scheme has to be Correct in order to convert additional sugars into glucose

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Fermentation with Enogen Corn

• Amazing Kernel is full of Goodies

• Utilizing Protein in the Kernel as a portion of our N source for Fermentation

• Displacing Urea with Protease on a Dollar to Dollar Basis resulting in improved yeast health and more consistent fermentations

• Impact to Yeast

– 20% Increase in Viability

– 20% Increase in Budding

– Healthy and Robust

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Reference Data:

+100 Ferms at 35% Solids

35% Solids Solids % DP-4 DP-3 DP-2 Glucose Lactic Glycerol Acetic Etahnol % TRS

Average 34.93 0.44 0.19 0.65 0.06 0.10 1.31 0.06 15.01 1.35

Std Dev 0.21 0.05 0.02 0.04 0.06 0.02 0.05 0.01 0.17 0.10

Min 34.06 0.39 0.16 0.48 0.02 0.07 1.22 0.05 14.53 1.2

Max 35.42 0.63 0.28 0.75 0.31 0.21 1.41 0.08 15.39 1.72

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Viscosity Reduction and New Technologies

• Particle Size Reduction Technologies– First Trials at Facilities with New Technologies – Appears to be some Synergies between Enogen and These

technologies– Further Reduction In Residual Starch – Improved Yield

• Oil Separation Technologies– Initial Experiments and Trials show Enogen appears to release more

Oil for Extraction– Initial Trial Results combining New Particle Size Reduction

Technologies and Enogen further increase oil extraction

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That Little Kernel – is full of little Goodies!

Cellulosic Ethanol Production

• Great Fiber Source already on hand

• ~ 10% of the Kernel is Fiber

• Syngenta believes Enogen Corn will help advance the next steps in the Renewable Fuels Industry

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Summary

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Enogen Corn Goes Beyond Liquid Alpha

Amylase Enzyme Replacement

•Processing flexibility

•Reduced energy and water use

•Reduced chemical usage

•Increased throughput and yield potential

•Intangibles

Value drivers at ethanol plant:

Business Confidential - Do Not Distribute

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Enables reduced inputs and increased outputs –increasing profitability

Local growers receive an additional 40¢ per bushel (on average)

≥ 10% reduction in carbon footprint and GDP impact (≈$2.2M in premiums¹)

Enogen: WIN – WIN – WIN

®As of July 25, 2013

Meet your New Enzyme Rep! (Enogen Corn Grower)