b iomaterials l ab i ntroduction and s afety wednesday, january 18 th bme 245/445 – lab 0

BIOMATERIALS LAB INTRODUCTION AND SAFETY

Wednesday, January 18th

BME 245/445 – Lab 0

PREFACE

The goal of this course is to provide students with hands on experience in…

Materials synthesis Materials characterization Materials modification Cell culture Histological Analysis Technical Writing & Data Analysis

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EXPECTATIONS

SAFETY! arrive prepared for lab work in TEAMS of 4-5 to complete lab write INDIVIDUAL lab report provide constructive feedback

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OVERVIEW

Safety Lab Outline Expectations Reports

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SAFETY

Environmental Health & Safety Training for Biological/Chemical Research Labs

labs are dangerous; so be safe if you have questions…ASK DON’T BE STUPID

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CHEMICAL HAZARDS

Flammable / Combustible / Toxic Respect all chemicals in lab

If you can smell it…it is bad for you! Use a hood if specified

LABEL EVERYTHING!

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BIOHAZARDS

Biosafety Level 1 – least hazardous Cell Culture Media Preparation

LABELS!

(krishgen.com)

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PROTECTIVE EQUIPMENT

Goggles – on at ALL times! Gloves – use them for everything Closed-toe shoes

Safety Quiz

(sentrysafetysupply.com)

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BIOMATERIALS LABS

1. Biomaterial Mechanics

2. Bulk vs. Surface Degradation

3. Cell-Material Interactions

4. Controlled Delivery and Release

5. Histological Analysis of Implanted Biomaterials

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Investigate how hydrogel precursors influence end-stage network mechanics

1. BIOMATERIAL MECHANICS

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Investigate different network chemistries and how these influence degradation

2. DEGRADATION MECHANISMS

(Wikipedia - Anhydride. 2011)

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3. CELL INTERACTIONS

Investigate how hydrogel incorporated “RGD” peptide influences cell adhesion

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(U. Michigan ME – Jianping Fu. 2011)

4. DELIVERY AND RELEASE Investigate how hydrogel properties can be

modified to control drug delivery

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Small Mesh Size Large Mesh Size

5. HISTOLOGICAL ANALYSIS OF

IMPLANTED BIOMATERIALS

Investigate how tissue responds to implanted biomaterials

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(Heungsoo Shin et al. Biomaterials. 24(19) 2011)

PRE-LAB

Literature reading relevant to lab Calculations Brief quiz at start of lab Introductory “lecture”

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POST-LAB REPORTS

Present findings in clear/concise fashion Make connections between lab experiments

and pre-lab literature reading List team members Error analysis REFERENCES! See Write-up Expectations on Blackboard

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For Lab #1

Register: Mon or Tues only Complete safety quiz; bring to lab (104)

Read paper on Blackboard Read lab handout Do pre-lab calculations