bio 328 immunology immunoglobulins: structure and function
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Bio 328 Immunology
Immunoglobulins: structure and Function
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Albumin
globulin
globulin
globulin
Origin
Inflammation Normal Normal Myeloma
Arne Tiselius and Elvin Kabat 1939.
2014)
1972 Noble Prize in Medicine.
Porter et al.
Nisonoff et al.
Edelman et al.
Goat anti-Human Fab binds to both L- and H-chain; goat antiHuman Fc binds
only to H-chain.
Angiotensin ll
Van der Waals surface contact between angiotensin ll and Fab fragment.
Flu virus antigen and antibody
Fab and Influenza virus antigen
Anti-HIV protease Fab
+ peptide
Fab different anti-hen egg lysozyme
Fab different small epitopes
Unbound Ag-bound
Induced-fit
Unbound Ag-bound
Anti-hemagglutinin Fab and peptide.
Antibody –Mediated Effector Functions
1.Ab-medicated clearance of pathogensa) Neutralizationb) Opsonizationc) Complement activationd) ADCC
2.Ab isotypes or classes mediate different functions3.Fc Receptors
How do Antibodies kill Cancer Cells?
1.Binding to cell surface receptors and preventing their activation.2.Interference with vascularization.3.Induction of apoptosis.4.Induction of ADCC.5.Induction of Complement fixation.6.Delivery of toxins.
IgG, IgA, IgD IgM, IgE
CH1/CH1CH1/CH1
Hinge regionCH2/CH2
CH2/CH2CH3/CH3
CH3/CH3CH4/CH4
1921 K. Prausnitz and H. Kustner1966 K and Y Ishizaka
Immunoreceptor Tyrosine Activation Motifs
B-cell Linker Protein
Bruton’s tyrosine kinase
X-linked agammaglobulinemia
The End
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