bioengineering: making life from scratch
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Bioengineering: making life from scratch
Melanie SwanMS Futures Group+1-650-681-9482
m@melanieswan.comhttp://www.melanieswan.com
Slides: http://slideshare.net/LaBlogga/slideshowsNIH, Bethesda MD
September 25, 2008
Must build it to understand it
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Synthetic biology
Novel biological system
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The new biology: virtuous feedback loop
Model and simulate
Investigate and
experiment
Build
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Bio-SPICE
Biological Simulation Program for Intra- and Inter-Cellular Evaluation
http://biospice.org
Model and
simulate
5
Registry of Standard Biological Parts Build
http://partsregistry.org
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Parts Registry: select measurement device
http://partsregistry.org
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Parts Registry: obtain part sequence
DNA Synthesizer
http://partsregistry.org
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The story of biocomputing
Mapping genes to proteins -> life can be engineered Genes can be turned on and off -> gene as binary switch Systemic understanding of DNA -> genes as circuits Cell noisiness -> need for stable and predictable circuits
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Repressilator schema“Noisy” cells
Michael Elowitz
Stanislas Leibler
Repressilator
A synthetic oscillatory network of transcriptional regulatorsMichael B. Elowitz & Stanislas Leibler
Nature. 2000 Jan 20;403(6767):335-38.http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
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Construction of a genetic toggle switch in Escherichia coliGardner TS, Cantor CR, Collins JJ
Nature. 2000 Jan 20;403(6767):339-42.http://www.ncbi.nlm.nih.gov/pubmed/10659857
Toggle switch induction threshold
Toggle switch plasmid
Gardner Cantor Collins
Genetic toggle switch
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Open wetware
Drew Endy
Parts Registry BioBricks Foundation iGEM competition
http://openwetware.org/http://bbf.openwetware.org/http://www.igem.org/http://partsregistry.org
12Genome
Bacterial image Genetic map
Craig Venter
Synthetic life
Synthetic life Fourth-generation biofuels
Mycoplasma genitalium
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Further applications of bioengineering
Coli-roid microbial camera
Landmine sensing plant: Arabidopsis thaliana
Amorphous computing
Natural genetic engineering
Ciliate
Cellular colonies
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Summary
Model and simulate
Investigate and
experiment
Build
Thank you
Melanie SwanMS Futures Group+1-650-681-9482
m@melanieswan.comhttp://www.melanieswan.com
Slides: http://slideshare.net/LaBlogga/slideshowsNIH, Bethesda MD
September 25, 2008
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