classroom genetics with wisconsin fast plants® - carolina biological

Carolina Biological Supply Company

Classroom Genetics with Wisconsin Fast Plants®

To Know a Plant, Grow a Plant

Plant a seed

In the mind and with the hand

Use the five senses

To engage and understand.

To investigate: observe,

Measure, analyze, and describe,

Harvest a Fast Plant seed

And make science come alive.

www.fastplants.org

Fast Plants® Relatives

Family Brassicaceae

Wisconsin Fast Plants®

• An entire life cycle in about 40 days

• Ideal for inquiry-based science investigations and for plant biology studies

• Flower 14–18 daysafter planting the seeds

Flower Structure of Brassica rapa

Pollination with Bee-Stick

Seed to Seed in 45 Days!

Ecological Relationships

• Quad

• Place wicks in quad

• Lay down paper before filling quads with soil

• Pre-wet soil

• Pack soil “medium firm”

• Fill ¾ full

Planting Wisconsin Fast Plants® (part 1)

Wicks

• Add 2–3 fertilizer pellets per cell

• Add more soil, then . . .

• Press potting mix in each cell before adding seeds

• Add 2–3 seeds per cell

• Cover lightly with potting mix

Planting Wisconsin Fast Plants® (part 2)

Mendelian Genetics with Brassica rapa

• A genetically controlled mating systemprevents self-fertilization

• Ideal for studies on the nature and inheritance of variation

• Distinctive mutant phenotypes

Readily Observable Phenotypes

(left to right) Rosette, dwarf, elongated, internode

Anthocyaninless (anl)

Yellow-green leaf (ygr1)

Monohybrid Crosses

ANL/ANLanl/anl(hairless)

Purple pigment is dominant to nonpurplepigmentation

(ANL) (anl)

P1 Generation

F1 Generation

Parent and F1 Generation

Observe parent and F1-generation petri dishes and hypothesize what the F2-generation phenotypes will be.

F2 Generation

Count the phenotypes.# of Purple _______# of Green _______

Did you hypothesize correctly?

F2 Generation

NonPurple Purple

A Tool for Inquiry in Mendelian Genetics

Observe P1- and F1-generation petri dishes and hypothesize what the F2-generation phenotypes will be.

P1 F1

Phenotype? Phenotype?

Now observe phenotypes of the F2

plates.

Rosette-Dwarf x Non-Purple Stem

A Tool for Inquiry in Mendelian Genetics

F2 Phenotypes

9:3:3:1

Normal Height Purple StemNormal Height Green StemDwarf Purple StemDwarf Green Stem

ros/ros

Rosette-Dwarf (15 days old)

• Gibberellin-deficient mutant

• Compact due to shortened internodes

• Attains normal height after application of 100 ppm gibberellicacid

• Flowers in 18 days

Phenotype Due to a Recessive Gene

Rosette-Dwarf x Non-Purple Stem

• F1 has normal height and purple pigment

• Genotype is ros/ROSanl/ANL

• Vehicle to study dihybrid genetics

Rosette-Dwarf x Non-Purple Stem

Rosette-Dwarf x Non-Purple Stem

• Mendel’s law of segregation and law of independent assortment

• Inheritance of 2 traits

• How genotypes influence phenotypes

• Scientific inquiry, including interpretation of evidence

Objectives for Application

Resources from Carolina

Wisconsin Fast Plants®

72-Hour Genetics Kit

3, 2, 1—Countdown to

Genetics

Monohybrid Cross Dihybrid Cross

Resources from Carolina

More Wisconsin Fast Plants® genetics kits:

• AP® Biology Alternative Lab 7: Genetics of Plants Kit (catalog no. 746537)

• Wisconsin Fast Plants® Dihybrid Genetics Kit (catalog no. 158772)

• Wisconsin Fast Plants® Genetics of Hairy Plants Kit (catalog no. 158785)

Resources from Carolina

Wisconsin Fast Plants® videos from Carolina:Go to Carolina.comTeacher ResourcesEducational Videos

More online resources:www.fastplants.org

Carolina Free Resources

Carolina offers many free resources to help support teachers.

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Thank you for investing your time in our training program.

For all of your classroom needs, check out our Web site, www.carolina.com.

Enjoy the rest of the conference!

Carolina Biological Supply Company