clonal propagation of bambusa vulgaris schrad ex wendl by leafy branch cuttings

Clonal Propagation of Bambusa vulgaris Schrad ex wendl by Leafy Branch Cuttings

M. A. Hossain

University of Chittagong, Bangladesh Korea University, Korea

Bamboos in BangladeshBamboos in Bangladesh

33 Species: 7 wild species in forests 26 domesticated

Bambusa vulgaris (Baijja Bansh): most widely used bamboo species in rural Bangladesh

Widely used in Building construction House making Agricultural implements Fishing traps Improvised bridge on small canel Making household products Bambusa vulgaris groove

Propagation methods..Propagation methods..

Do not set seed after flowering, no seedling progeny

Propagate only through Vegetative methods

Low survival rate

Bulkiness of (20-30Kg) of rhizomes

Expensive option

1. Rhizome cutting

Rhizome cutting

Easiest method

Limited only to wet seasons

Very few branches develop root at their base

2. Pre-rooted branch cutting

(Banik,1997 )

Propagation methods..Propagation methods..

3. Secondary branch cutting

Hossain et al. J. Bamboo and Rattan, 2005, 4 (3), 231–241

Propagation methods..Propagation methods..

4. Primary and Secondary branch cutting

Hossain et al., J. Bamboo and Rattan, 2006, 5 (3&4),117-126

Propagation methods..Propagation methods..

Convenient The numbers of suitable branches (>5mm dia.) to produced root are

inadequate. Limited only to rainy season during June-July.

Propagation by Leafy Branch Cuttings Propagation by Leafy Branch Cuttings

Regeneration from leafy brunch cuttings

- Nodal leafy cuttings

- Tip cuttings

Effect of IBA on rooting ability of leafy branch cuttings

- 0%, 0.1%, 0.4% and 0.8% IBA Solution

Nodal leafy cuttings Tip cuttings Non-mist propagator 4-6 weeks

Rooted cuttingsCutlings in polybag (2 months)

Propagules in nursery bed (6 months old )

Propagule ready for planting

Experimental procedureExperimental procedure

Results..Results..

0

10

20

30

40

50

60

70

0% 0.1% 0.4% 0.8%

IBA concentrations

Ro

oti

ng

per

cen

tag

eNodal leafy cuttings

Tip cuttingsa

cd c

b b

c

d

e

Rooting percentage

0

2

4

6

8

10

12

0% 0.1% 0.4% 0.8%

IBA concentration

Ro

ot

nu

mb

er

Nodal leafy cuttings

Tip cuttings a

b

c cb bc

e

d

Root number

Nodal leafy cuttings

Tip cuttings

Results..Results..

Cutting types IBA concentration p

0% 0.1% 0.4% 0.8%

Nodal leafy cuttings

10.1±0.41 a 13.9±0.95 a 14.0±0.74 a 12.8±2.03 a NS

Tip cuttings 10.2±0.70 a 12.1±2.09 a 12.0±1.68 a 9.9±0.44 a NS

Root length

Results..Results..

50

60

70

80

90

100

0% 0.1% 0.4% 0.8%

IBA concentration

Su

rviv

al p

erc

ent

age

Nodal Leafy cuttings

Tip cuttingsa a

bcd

abcab

bcd

d

cd

Survival percentage

Nodal leafy cuttings

Tip cuttings

Results..Results..

Growth performance

Nodal leafy cuttings Tip cuttings

Results..Results..

Nodal leafy cutting and tip cuttings, both produced roots, survived and formed rhizome.

The highest rooting percentage and root number per cutting was in 0.8% IBA treated cuttings.

IBA treated cuttings showed better survival capacity in the nursery condition over controlled one.

The result presented here could help in opening a new avenue for the mass propagation of this variety bamboo species.

Summary of findingsSummary of findings

Further aspectsFurther aspects

The rooting percentage of leafy branch cuttings of B. vulgaris in this study was 56. There is scope to increase the rooting ability of cuttings of the species.

Furthermore, the performance of planting stock developed through the methods was not assessed in field condition. This might be one of the important aspects for future study.

Acknowledgement

International organizing committee of WBC

University of Chittagong, Bangladesh

Bangladesh Forest Research Institute

Korea University, Korea

Thank You