comparative diagnosis of canine parvovirus with … · 2017. 2. 2. · comparative diagnosis of...

COMPARATIVE DIAGNOSIS OF CANINE PARVOVIRUS WITH METHODS OF RAPID TEST

AND ELISA

Irmak DIK and Atilla SIMSEK

Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey

“5th Animal Health and Veterinary Medicine Congress” September 26-27, 2016 Valencia, Spain.

• Canine parvoviral enteritis is an acute highly contagiouslife-threatening infection and therefore, laboratorialdiagnosis is essential for screening diarrheic puppies inorder to prevent infection of susceptible contact animals.

INTRODUCTION

CPV

• Canine parvovirus 2 (CPV-2) is the causative agent of acutehemorrhagic enteritis and myocarditis in dogs and it is oneof the most important pathogenic viruses.

• It is very contagious and has a high morbidity and mortalityrate in domestic and wild canines.

• It especially affects youngdogs that are notprotected by maternalantibodies or vaccination.

• The disease is characterized by two prominent clinicalforms ;

� enteritis with vomiting anddiarrhea in dogs of all ages

� myocarditis and subsequentheart failure in pups of less than3 months of age

• Virus isolation (VI) and Electronmicroscopy (EM) are more sensitive,but they are too labor-intensive andtime-consuming for routinediagnostic testing.

• Routinely, faeces from diarrhoeicdogs are screened using ELISA andImmunochromatographic test (IC)(Rapid Test)

CPV

IC ELISA

• It has been hypothesized that was to compare the IC orELISA currently used for CPV diagnosis.

MATERIAL AND METHODS

• In the present study, a total of 100 fecal samples fromunvaccinated diarrheic puppies of less than 1 years,collected by;

� Veterinary Medicine Hospital of Selcuk University,

� Konya Municipality Dog Shelter

• They were analyzed for CPV antigens by IC test and ELISA.

• IC test was carried out with acommercial kit (Agrolabo, İtaly) following themanufacturer’s instructions.

• The IC test were performedimmediately after fecal samplecollection.

• The feces samples were tested for presence of CPVantigens using ELISA (Agrolabo, İtaly)

• The tests were performed as per the manufacturer’sinstructions.

• The plates were then read on an automatic micro platereader (Rayto RT 2100C, China).

RESULT

� In total, 24 feces samples were determined to be CPVantigen positive.

� 16 feces samples were identified as positive for CPVantigens by both ELISA and IC test and 8 feces sampleswere positive only in the IC test.

� ELISA 16%� IC Test 24%

• The all data were are not statistically significance. Theresults were analyzed using analysis by SPSS 19.0.

• In all cases, P < 0.05 was the criterion for statisticalsignificance.

DISCUSSION

• Parvoviral infections in dogs have become an importantproblem globally.

• The clinical signs resembleother enteric diseases andhence rapid and earlydiagnosis of the condition hasbecome ever more urgent.

• In-clinic tests are rapid assayssuitable for the diagnosis of CPV inthe veterinary practice, thusrepresenting the only tests availablefor a rapid diagnosis under fieldconditions.

• A rapid diagnosis of CPV infection is especially important inkennels and shelters in order to isolate infected dogs andprevent secondary infections of susceptible contactanimals.

• Clinical diagnosis is indecisive and several other viralpathogens may cause diarrhea in dogs, such ascoronaviruses, adenoviruses, morbilliviruses, rotavirusesand reoviruses (Decaro and Buonavoglia 2011).

• But some laboratory diagnostic methods are time-consuming and expensive.

• The IC test is most common rapid field diagnostic methodused in clinical practice.

• ELISA for detection of CPV in dog faecal samples hasshowed that the test is highly sensitive and specific and isamenable to field use (Esfandiari and Klingeborn 2000).

• ELISA and IC test are based on antigen and antibodyreactions.

• Some literatures; IC test and ELISA results are not reportedsignificant as statistically for a few infections (Reithinger et al. 2002).

However; the IC test are declerated to has very lowspecificity in some researches (Esfandıarı and Klıngeborn 2000).

• The use of the IC parvovirus test has several advantagesagainst ELISA, which can be exploited.

1. this test is a IC test and the test procedure is veryrapid and simple. Sample preparation and testperformance require no extra equipment. The testprocedure for ELISA requires at least 2–3 h.

2. the IC test can be undertaken in a large laboratory,at the veterinary practice or by the side of theanimals. Thus, the test can easily be performed in thesmall veterinary clinic as well as by the owner himself.The ELISA will only be undertaken in the laboratory bya laboratory technician.

3. the IC test can be stored at 4°C–25°C for 15months. This storage condition makes it anappropriate test method for ambulatory practice. TheELISA has to be stored at 2°C–8°C for 12 months (Esfandıarı

and Klıngeborn 2000.

• We concluded that the rapid test might be used instead ofELISA, as the rapid test is more determinative andeffective compared to ELISA for CPV infections.

• Therefore, the results of the two different test methodsare not statistically different by chi-square test in thecurrent research.