crimean congo hemorrhagic fever- pakistan perspective national institute of health,...
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Crimean Congo Hemorrhagic Fever- Pakistan Perspective
National Institute of Health, Islamabad-Pakistan
Lab Methodologies used at NIHRoutine/Current Diagnosis:• ELISA
Kits used: BDSL IgM Capture ELISA• RT-PCR
Reference protocol: “Polymerase Chain Reaction for Diagnosis and Identification of Distinct Variants of Crimean-Congo Hemorrhagic Fever Virus in the United Arab Emirates. American Journal of Tropical Medicien and Hygiene, 55(2), 1996, pp. 190-196”
• Genetic Sequencing:National Institute of Health, Pakistan holds the thorough capability including infrastructure and trained personnel but deficit of resources
Discontinued methodologies:• Electron Microscopy• Inoculation in suckling mice• Fluorescent Microscopy
Phylogenetic relationship of CCHF viruses detection during 2008-Quetta-Pakistan
Outbreak
2008 PAK viruses (denoted by●) with Closest homology to: Asia Type-I
Pakistan 1976, 2001 and 2002 viruses (denoted by ) as well as with Iran, Afghanistan
Under Publication
Future Needs for CCHF Lab Diagnosis
at HIH
• No recent changes to the lab diagnostic protocols
• Need to establish a separate Core laboratory facility early service provision under improved standards
• Establishment of standard guidelines for lab diagnosis
Safety Precautions being used at NIH
• BSL-II+ Laboratory with enhanced PPE
• Separate Designated areas for sample receipt, processing and testing
• Restricted personnel access• Waste management through
Autoclaving and incineration
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