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Cylindrocladium buxicola Research 2014: effective strategy

9 October 2014

© DLV Plant

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Index

• Introduction DLV Plant• Research location(s)• Background Cylindrocladium research• (Temporary) results.

© DLV Plant

DLV Plant

• Advice to primary enterprises in the garden andagricultural sector.

• Mainly company assistance.– Current developments at cultivation level– Developments at management level (strategy).

+ Mutual knowledge exchange between the consultants + Large number of companies assisted. + Active in almost all garden and agriculture sectors.

© DLV Plant

© DLV Plant

DLV Plant / arboriculture team

DLV Plant / research

© DLV Plant

DLV Plant / Research centre arboriculture

• Start 2007• Independent research in a neutral location.• Practice-orientated and possibility to

carry out risk free tests in:

– Greenhouse– Container field– Outdoors

» Crop protection » Fertilisation » Authorisation research » Innovations

© DLV Plant

Cylindrocladium in Buxus: Background

© DLV Plant

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Cylindrocladium in Buxus: Background

• Young fungus• Very infectious• Aggressive / infection after 5 hours• Development at 5°C to 30°C• Preference for humid to very wet conditions• Way of life and distribution unknown• Limitation crop protection package to combat the

illness

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Cylindrocladium: Test 2014 / Introduction

Aim: development of a Cylindrocladium buxicola prevention strategy.

Method: provide measures in the shape of remedies or otherwise.

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Cylindrocladium: Test 2014 / Participants

• Manufacturers crop protection• Manufacturers plant boosters / fertilisers• Growers

© DLV Plant

© DLV Plant

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Cylindrocladium: Test 2014 / Strategy

1. Untreated: untreated2. Reference: chemical with spray sulphur3. Manufacturer 1: crop protection agents4. Manufacturer 2: crop protection agents5. Manufacturer 3: crop protection agents6. Grower X: product Y and product Z7. Top buxus: Product A and product B

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Cylindrocladium: Test 2014 / Strategy

• Product A: Straw 10 ton / ha (1 kg/m²).

• Product B: Code HM

© DLV Plant

© DLV Plant

Cylindrocladium: Test 2014 / Test plan

© DLV Plant

Cylindrocladium: Test 2014 / Test plan

© DLV Plant

Cylindrocladium: Test 2014 / Test plan

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Cylindrocladium: Test 2014 / Treatments

Date 15-5-2014 30-5-2014 19-6-2014 7-7-2014 22-7-2014 31-7-2014 12-8-2014 29-8-2014 17-9-2014 Time 14:00 9:00 20:00 13:00 9:00 20:00 14:00 10:00 10:00

Object 2 Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Object 3 Merpan (*1)

Merpan

(*1)

Spirit (*1) Spirit (*1) Spirit (*1) Spirit (*1) Merpan

(*1)

Merpan

(*1)

Merpan

(*1)

Object 4 Flint/Spray sulphur

A13703

Flint/Spray

sulphur

A13703

Flint/Spray

sulphur

A13703

CODE P

(*1)

A9219 /

CODE P

(*1)

A9219 /

Folicur /

Silwet

A9219 /

Folicur /

Silwet

A9219 /

CODE P

(*1)

A13703

CODE P

(*1)

A13703

Object 5

(*1)

(*1)

(*1)

Silwet

Gold (*2)

Silwet

Gold (*2)

Silwet

Gold (*2)

Agral Gold

(*2)

(*1)

(*1)

Object 6 CODE HM

(*3)

x CODE HM

(*3)

x CODE HM

(*3)

CODE HM

(*1)

CODE HM

(*1)

x CODE HM

(*3)

Object 7 CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

© DLV Plant

Cylindrocladium: Test 2014 / Treatments

Date 15-5-2014 30-5-2014 19-6-2014 7-7-2014 22-7-2014 31-7-2014 12-8-2014 29-8-2014 17-9-2014 Time 14:00 9:00 20:00 13:00 9:00 20:00 14:00 10:00 10:00

Object 2 Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Object 3 Merpan (*1)

Merpan

(*1)

Spirit (*1) Spirit (*1) Spirit (*1) Spirit (*1) Merpan

(*1)

Merpan

(*1)

Merpan

(*1)

Object 4 Flint/Spray sulphur

A13703

Flint/Spra

y sulphur

A13703

Flint/Spra

y sulphur

A13703

CODE P

(*1)

A9219 /

CODE P

(*1)

A9219 /

Folicur /

Silwet

A9219 /

Folicur /

Silwet

A9219 /

CODE P

(*1)

A13703

CODE P

(*1)

A13703

Object 5

(*1)

(*1)

(*1)

Silwet

Gold (*2)

Silwet

Gold (*2)

Silwet

Gold (*2)

Agral Gold

(*2)

(*1)

(*1)

Object 6 CODE HM

(*3)

x CODE HM

(*3)

x CODE HM

(*3)

CODE HM

(*1)

CODE HM

(*1)

x CODE HM

(*3)

Object 7 CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

9 Treatments

© DLV Plant

Cylindrocladium: Test 2014 / Treatments

Date 15-5-2014 30-5-2014 19-6-2014 7-7-2014 22-7-2014 31-7-2014 12-8-2014 29-8-2014 17-9-2014 Time 14:00 9:00 20:00 13:00 9:00 20:00 14:00 10:00 10:00

Object 2 Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Spray sul-

phur (*1)

Object 3 Merpan (*1)

Merpan

(*1)

Spirit (*1) Spirit (*1) Spirit (*1) Spirit (*1) Merpan

(*1)

Merpan

(*1)

Merpan

(*1)

Object 4 Flint/Spraysulphur

A13703

Flint/Spra

y sulphur

A13703

Flint/Spra

y sulphur

A13703

CODE P

(*1)

A9219 /

CODE P

(*1)

A9219 /

Folicur /

Silwet

A9219 /

Folicur /

Silwet

A9219 /

CODE P

(*1)

A13703

CODE P

(*1)

A13703

Object 5 (*1) (*1) (*1) Silwet

Gold (*2)

Silwet

Gold (*2)

Silwet

Gold (*2)

Agral Gold

(*2)

(*1) (*1)

Object 6 CODE HM

(*3)

x CODE HM

(*3)

x CODE HM

(*3)

CODE HM

(*1)

CODE HM

(*1)

x CODE HM

(*3)

Object 7 CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

CODE Y /

CODE Z

6 Treatments! © DLV Plant

Cylindrocladium: Test 2014 / observations

• Daily: QMS® check infection pressure forecast.

• Weekly: observation round / scouting.

• Monthly / when high infection pressure: counts.

© DLV Plant

Cylindrocladium: Test 2014 / observations

Class Description Information 0 Healthy crop Not a single contaminated leaf visible 1 Very lightly contaminated Clearly visible contamination, but not more than five

leaves per plant. 2

Lightly contaminated

3 Moderate contamination

4

Clearly visible contamination and easy to count. 5 to 10 leaves are clearly contaminated. Clearly visible contamination and easy to count. Number of contaminated leaves between 10 and 15. Counting leaves becomes more difficult and it is estimated that no more than 20 leaves are contaminated.

5 Heavy contamination More than 20 leaves are contaminated. This is hard to count and is an estimate.

6 Counting is impossible. It is estimated that 5 to 10% of the crop is ill or loses leaves.

7 Very heavy contamination. 10 – 30 % contamination or lost leaves per plant.

8 30 – 50% contamination or lost leaves per plant. 9 Extremely heavy contamination. 50 – 100 % contamination or lost leaves per plant.

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© DLV Plant

Cylindrocladium: Test 2014 / observations

12345678910 11 12

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Cylindrocladium: Test 2014 / observations

Frequency 12345678910 11 12

25%

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7

Cylindrocladium: Test 2014 / observations

Frequency 12

Intensity 345

3*12 leaves = 3% 6

3* 40% = 10% 8910 11 12

25%

© DLV Plant

Cylindrocladium: Test 2014 / Result

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© DLV Plant

Cylindrocladium: Test 2014 / Result

Extra treatment carried on 31-7

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Cylindrocladium: Test 2014 / Result

Regular treatment on 12-8

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Cylindrocladium: Test 2014 / Result

Contamination < 10%

Cylindrocladium: Test 2014 / Conclusion

• 30% less spraying.• Better than most chemical

treatments.

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Cylindrocladium: Test 2014 / Conclusion

Thank you for your attention.

© DLV Plant

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