deliverables: peptide mapping - acroscale · 2017. 12. 21. · deliverables: peptide mapping...

http://www.acro-scale.co.jp/Acroscale Inc. Sendai laboratory +81-22-752-2211 acro-docketing@acro-scale.co.jp

D E L I V E R A B L E S : P E P T I D E M A P P I N G

Enz ymatic d igest ion , LC/MS measurements and analys is per formed

Measurement ser v ice de l iverable

Chromatogram: T IC (U V )

(E�ciency and sample amount cannot be guaranteed)

CharacterizeLC / MS

P r e - p r o c e s s i n g s e r v i c e M e a s u r e m e n t A n a l y s i s s e r v i c e

Chromatogram / Spectra

F u l l c h r o m a t o g r a m

Our measurement se r v ice prov ides chromatograms and spectra as R AW data

EnzymatictreatmentDenature

ReportRaw dataGlycoproteinPeptide

Glycopeptide

Del iverables

A n a l y s i s s e r v i c eS equence ID Repor tR AW dataProcessed sample

(Chromatogram, Spectra, fragment labeling (sequence identification), coverage, etc analysis to identify the di�erent parts of the sequence)

A n a l y s i s s e r v i c eGlycosylation Site Report

Estimation of glycosylation sites(Inspection of di�erent locations on the sequence

and estimation of glycosylation site(s))

S p e c t r a

X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X

http://www.acro-scale.co.jp/Acroscale Inc. Sendai laboratory +81-22-752-2211 acro-docketing@acro-scale.co.jp

D E L I V E R A B L E S : P E P T I D E M A P P I N G

Analys is ser v ice de l iverable: Sequence identification

F u l l c h r o m a t o g r a m : T I C

Our analysis service includes chromatogram, spectra, fragment labeling (sequence identification), coverage, etc analysis to identify the di�erent parts of the sequence.

1

2

3

Peptide fragmentidentification

Analys is ser v ice de l iverable: Est imation of g lycosy lat ion pos it ion

Full chromatogram comparison: TIC

Amino acid sequence coverage:

Di�erent sites on the sequence inspected and glycosylation site(s) estimated

Non-g l ycos y lated p rote in

G l ycos y lated p rote in

Chromatogramcomparison

# # %

Fr a g m e n t l a b e l i n g

S p e c t r a