diaphanosoma celebensis and its feeding to marine fish ... · mass culture of euryhaline cladoceran...
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Mass Culture of Euryhaline Cladoceran Diaphanosoma celebensis and its
feeding to marine fish larvae
A H i T N k t Y I dA. Hagiwara, T. Nakamoto, Y. Inada, I. Maruyama, and Y. Sakakura
Nagasaki Univesity, Japan g y p
ObjectiveObjective
O i l i t bilit i A t i t• Occasional instability in Artemia cyst harvests, prices and quality
• To seek for other crustacean plankton pas an alternative to Artemia.
• To develop intensive mass cultureTo develop intensive mass culture method.
Cladoceran species for intensive mass culture
Moina macrocopa (Nakamoto et al.,2007,2008)
Di h l b i( , , )
Diaphanosoma celebensis (Segawa & Yang, 1987; de la Pena et al., 2001;
Marcial & Hagi ara 2007)Marcial & Hagiwara, 2007)
Moina mongolica (He et al., 2001; Wang & Lee, 2004)
Total length ± range (um) of Artemia and cladoceran, and swimming speed after they are transferred to larval rearing tank
10
swimming speed after they are transferred to larval rearing tank (33 ppt in salinity)
68
10
peed
46 Artemia
DiaphanosomaFreshwater Moinam
ing
sp/s
ec)
02
200 400 600 800 1000 1200 1400
Freshwater Moina
Sw
imm
(mm
/
200 400 600 800 1000 1200 1400Size (um)
L i i ti f lt d l d ill i b tt f dLarger size variation of cultured cladoceran will give better food environment for fish larvae by enhancing food selectivity of larvae (Tanaka et al., 2005)
Effect of temperature and salinity on the population growth of D celebensis (n=6)growth of D. celebensis (n=6)
Effect of aeration rate on the dissolved oxygen concentration and population growth of D. celebensis
100
)
15 Den
0 mL/min DO Density450 mL/min 100 15
DO Density
50
DO
(%
5
10
nsity (Ind./ml)
50
DO
(%
)
5
10
Density (Ind./m
l)
100 15
00 2 4 6 8 10 12 14 16 18 20
0
30 mL/min
00 2 4 6 8 10 12 14 16 18 20
0
50
100
DO
(%
)
5
10
15 Density (Ind.
00 2 4 6 8 10 12 14 16 18 20
Days
0
5 ./ml)
0, 30 mL/L450 mL/LDays
Swimming pattern
Effect of the addition of chicken manure extract on the population growth of D. celebensis (mean+SD, n=3)
Chicken manure extract
Reproduction on D. celebensis exposed to 17β-estradiol (ug/l) (Marcial and Hagiwara, 2007)
Parent8 60
*
* *F
Dunnett’s test p<0.05n = 8
*
4
6
8
30
45
60* *
* **on
(day
)
emal
e)
*
4
6
8
20
30
40* *
***
F1
0
2
0
15
prod
uctio
fspr
ing/
fe
0
2
0
10
t firs
t rep
ndity
(off
84050
** *
F2
6
82025F3
Age
at
Fecu
n
2
4
6
10203040* *
* * * 2
4
6
51015
0
Treatment
0 0
Treatment
0
S h i hi k t t (CME)Sex hormones in chicken manure extract (CME) and D. celebensis
CME-1 CME-2 CME-3 D. celebensis17alpha estradiol 0 13 ND 0 19 ND17alpha-estradiol 0.13 ND 0.19 ND17beta-estradiol 0.62 0.22 0.91 NDEstron 0.71 2.2 4 NDEstriol ND ND ND NDProgesterone ND ND ND NDTestosteron ND ND ND NDTestosteron ND ND ND NDMethyltestosterone ND ND ND ND
M. macrocopa Mass Culture System using triple pipe aeration equipment (Nakamoto et al., 2007)
Condensed Chlorella
M. macrocopa
M. macrocopa culture system Improved system for Diaphanosoma using oxygen supply
Highest density 62.4 ind./ml (100L tank)g y ( )
1950 g wet weight
D celebensis Mass Culture SystemsD. celebensis Mass Culture Systems
Batch culture system using 100 L t k
Closed recirculating system100 L tank
Enrichment by Schizochytrium for 6 h
1800
2000
Artemia
1200
1400
1600
00gD
W) Artemia
D. celebensis
800
1000
1200
ent (
mg/
1
200
400
600
Con
te
0
200
c 14:0
14:1
c 16:0
16:1
16:2
c 18:0
18:1
18:2
18:3
c 20:0
20:1
20:4
20:5
c 22:0
22:1
22:5
22:6
c 24:0
24:1
Fatty acids
Population growth of D celebensis
(×104)
Population growth of D. celebensis in 30 L culture for feeding fish larvae
100120
80
100(×10 )
406080
40
60
Num
ber C
ulture v
02040
0
20
0 10 20 30 40 50 60 70 80
volume (L
Total number Culture volume
L)
Days
Trial with Japanese flounderday 3 (mouth opening) day 24 (beginning of metamorphosis) day 47 (beginning of settlement)
RotiferArtemia
D. celebensis
100 L (44 cm diameter, 70 cm hight; Ruttanapornvareesakul et al. 2007 Aquacult. Res.)18 ºC, 10L:14D, aeration rate 50 mL/min
Japanese flounder on day 47
A t i D l b iArtemia D. celebensis
Survival (%) 71.4±9.5 67.1±11.7
Total length (mm) 15.8±0.9 16.0±1.6
S ttl t (%) 21 3 19 5Settlement (%) 21.3 19.5
ConclusionConclusion• Highest density of D. celebensis in 100L culture was 62 g y
ind./ml at 25C, 10ppt Sal., 0L:24D by feeding condensed freshwater Chlorella.
• Water flow management and chicken manure treatment was effective for successful mass culture.
• Larviculture of Japanese flounder was successfully conducted by feeding D. celebensis.y g
• Production cost was 11.3 Euro/ kg wet weight.
Thank you !
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