effects of per on longevity and fecundity in drosophila james lester dr. jadwiga giebultowicz
Post on 21-Dec-2015
216 Views
Preview:
TRANSCRIPT
Effects of per on longevity and fecundity in Drosophila
James Lester
Dr. Jadwiga Giebultowicz
Dr. Giebultowicz’s research
Circadian Rhythms Clock Genes Tim Per Effects of expression
Increased longevitySurvival Curves
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
Days
Female Survival (percent)
wild-type
per01
per04
perR
UAS Gal4
UAS per
Gal4 x UAS per
Increased fecundity
Average Life Progeny
0.05,000.0
10,000.015,000.020,000.025,000.030,000.035,000.040,000.0
per01per04#58 #34 #33 #45 perR
Cs
#45 x #34#58 x #34#45 x #33#58 x #33Genotype
Eggs Laid (Thousands)
Copulating Drosophila
My research
Verify UAS Gal4 x UAS per results
Eliminate cross breeding as variable
Determine how per increases longevity and fecundity
Survival Curves
0
10
20
30
40
50
60
70
80
90
100
0 10 20 30 40 50 60 70 80 90 100
Days
Female Survival (percent)
wild-type
per01
per04
perR
Tim: Gal4
UAS per
Gal4 x UAS per
Cross-breeding
–UAS Gal4 x UAS per (over expressed)
–UAS per x White
–UAS Gal4 x White
–White x White
–Wper01 x Wper01 (no expression)
Possible Longevity Factors
Diet: How much food is eaten?How much is stored?
Ovaries: Do the ovaries develop at different rates?
Stress resistance:-Heat-Oxidative stress-Metabolic stress
Preliminary Metabolic Stress Results
Percentage of Drosophila alive after starvation
0
20
40
60
80
100
120
8 20 32 44 56 68 80 92 100 110
Hours starving
Percentage alive
CS 1
CS 2
Per01 1
Per01 2
Results
Aging of cross-bred and over expressed is ongoing…
Metabolic and oxidative tests are about to occur…
Aging of virgins
The fun has just begun!!!
top related