endocytosis - california institute of technologymbklab/courses/bi113/lecture 5.pdf · endocytosis...

Endocytosis

Three types of coated vesicles transport proteinsfrom organelle to organelle

Lodish et al, 2000

Lilly (1993) Pathophysiology of Heart Disease

A single layer of endothelial cells lines arteries

Lilly (1993) Pathophysiology of Heart Disease

Fatty streaks contain macrophage cells with lipid deposits

FibrousPlaques

Calcification

Lilly (1993) Pathophysiology of Heart Disease

Fibrous plaques damage the endothelial layer

Pathology Advances

Ulceration Thrombosis

Brown & Goldstein (1986) Science 232:34

Cholesterol is carried mostly aslow density lipoprotein (LDL) particles

HMGCoA controls the rate limiting stepin cholesterol synthesis

Zubay, 1998

Receptor-mediated endocytosisvia clathrin-coated pits and vesicles

Lodish et al, 2000

Brown & Goldstein (1986) Science 232:34

LDL receptor recycling pathway

EM image of clathrin-coated pitfrom cytosolic side

Lodish et al, 2000

Structure of a clathrin-coated vesicle

Lodish et al, 2000

Smith & Pearse. Trends in Cell Biology, 1999

movie by Allison Bruce

Assembly of clathrin coated vesicle

Assembly and vesiculation of clathrin-coated pits

Lodish et al, 2000

Dynamin is a GTPase required for pinching off ofclathrin-coated vesicles

Lodish et al, 2000

Components that participate inbudding of coated vesicles

Lodish et al, 2000