epigenetic modifications...epigenetic modifications copyright © 2018 abcam, all rights reserved....

Epigenetic modifications

Copyright © 2018 Abcam, All rights reserved.

Lpcat1

BBAP/DTX3L

HAT4

ARDT1

me

16

K

3

R1

S5

K

P mecit

o-p

bromo

ac

8

K

ac

12

K

ac

ac

ac

ac

20

K

47

S

91

K

me

MBT

tudor

PWWP bromo

ac

H4

adp

ub

SIRT2spSir2

PRMT1PRMT5PRMT6

CKIIScCK1 PADI4

hKAT1 (HAT1)scKAT1 (Hat1)spKAT1 (Hat1/Hag603)dKAT1 (CG2051)hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)hKAT5 (TIP60/PLIP)scKAT5 (Esa1)spKAT5 (Mst1)dKAT5 (dTIP60)hKAT7 (HBO1/MYST2)spKAT7 (Mst2)dKAT7 (CHM)

hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)hKAT5 (TIP60/PLIP)scKAT5 (Esa1)spKAT5 (Mst1)dKAT5 (dTIP60)hKAT7 (HBO1/MYST2)spKAT7 (Mst2)dKAT7 (CHM)

hKAT1 (HAT1)scKAT1 (Hat1)spKAT1 (Hat1/Hag603)dKAT1 (CG2051)hKAT5 (TIP60/PLIP)scKAT5 (Esa1)spKAT5 (Mst1)dKAT5 (dTIP60)hKAT7 (HBO1/MYST2)spKAT7 (Mst2)dKAT7 (CHM)

hKAT5 (TIP60/PLIP)scKAT5 (Esa1)spKAT5 (Mst1)dKAT5 (dTIP60)hKAT8 (hMOF/MYST1)scKAT8 (Sas2)spKAT8 (Mst2)dKAT8 (dMOF/CG1894)

hKMT5A (Pr-SET7/8)hKMT5B (SUV4-20H1)hKMT5C (SUV4-20H2)spKMT5 (Set9)dKMT5A (PR-set7)dKMT5B (Su(var)4-20)hKMT3B (NSD1)

RNF20/RNF40UBCH6ScRad6MST1

hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)

hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)

hKAT3AhKAT5B

ARDT1

5

K

ac 12

K

ac14

K15

K

ac

20

K

ac

30

K120

K

ubP adp

H2B

hKAT3B

NHK-1

ARTD1

1

S

H2A

P

120

T

P119

K

ub29

R

me13

K

adp11

R

me

3

R

me

5

K

ac

cit

PRMT1PRMT6

PRMT1PRMT6

Msk1PKC

PRMT6

PADI4

Bmi/Ring1A

hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)hKAT1hKAT5ScKAT5

hKMT6B (EZH1)hKMT6 (EZH2)hKMT3G (NSD)dKMT6 (E/(Z))

PRMT6

spMst1spSir2

hKAT3AhKAT5B

hPRMT4

hPRMT4

PRMT5

PKC beta

PADI4

PADI4

PADI4

PADI4

ARTD1

hKAT3B (p300)hKAT2A (hGCN5)hKAT2B (PCAF)scKAT2 (Gcn5)spKAT2 (Gcn5)dKAT2 (dGCN5/PCAF)

hJAK2

ARTD1

hKMT4 (DOT1L)scKMT4 (Dot1)

ac

P

10

S

14

K

28

S

27

K

26

R

38

P37

K

79

K56

K

45

T

P

41

Y

P

6

T

P

P

bromo

PHD

MBT

tudor

chromo

ZF - CW

PHD

MBT

chromo

BIR

ANKYRIN

PWWP

14-3-3

P14-3-3

H3

P

me

me

chromo

me

chromo

me

cit

cit

ac

adp

adp

iso

30

P

iso

meac

me

ac

36

K

me

me23

K

ac18

R

ac

ac

17

R

me

8

R

me

P

9

K

11

T

P

3

T

P

ac me

4

K

ac me

cit

2

R

me cit

MSK1MSK2

hHDAC1hHDAC2

hPKC-delta

42

R

Rv1988

hKAT3B (P300)scKAT11 (Rtt109)spKAT11 (Rtt109)hKAT2A (GCN5)

DLK/ZIPPRK1

HaspinVrk1

SETMARhKMT2A (MLL1)hKMT2B (MLL2)hKMT2C (MLL3)hKMT2D (MLL4)hKMT2E (MLL5)hKMT2F (hSET1A)hKMT2G (hSET1B)hKMT2H (ASH1)hKMT3C (SMYD2)hKMT3G (NSD3)hKMT7 (SETD7)scKMT2 (Set1)spKMT2 (Set1)dKMT2A (Trx)

hKMT3A (SET2)hKMT3B (NSD1)hKMT3C (SMYD2)NSD2scKMT3 (Set2)spKMT3 (Set2)Zfh Setd3

scFPR4scFPR4

KDM6A (UTX)KDM6B (JMJD3)

PP1

SIRT6

Aurora BPim1MSK1MSK2IKK alpha

hKAT2A (hGCN5)hKAT2B (PCAF)scKAT2 (Gcn5)spKAT2 (Gcn5)dKAT2 (dGCN5/PCAF)hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)hKAT6A (MOZ/MYST3)hKAT6B (MORF/MYST4)scKAT6 (Sas3)spKAT6 (Mst2)dKAT6 (CG1894)hKAT10 scKAT10 (Hap2)hKAT12 (TFIIIC90)

hKMT1A (SUV39H1)hKMT1B (SUV39H2)hKMT1C (G9a)hKMT1D (EuHMTase/GLP)hKMT1E (ESET/SETDB1)hKMT1F (CLL8)hKMT8 (RIZ1)spKMT1 (Clr4)dKMT1 (Su(var)3-9)

hKAT2A (hGCN5)hKAT2B (PCAF)scKAT2 (Gcn5)spKAT2 (Gcn5)dKAT2 (dGCN5/PCAF)hKAT12 (TFIIIC90)

hKDM1 (LSD1/BHC110)hKDM3A (JHDM2a)hKDM3B (JHDM2b)hKDM4A (JMJD2A/JHDM3A)hKDM4B (JMJD2B)hKDM4C (JMJD2C/GASC1)hKDM4D (JMJD2D)scKDM4 (Rph1)PHF8

hKAT2A (hGCN5)hKAT2B (PCAF)scKAT2 (Gcn5)spKAT2 (Gcn5)dKAT2 (dGCN5/PCAF)hKAT3A (CBP)hKAT3B (p300)dKAT3 (dCBP/NEJ)hKAT12 (TFIIIC90)

hKDM1 (LSD1/BHC110)spKDM1 (Swm1/Saf110)dKDM1 (Su(var)3-3)hKDM2B (JHDM1b/FBXL10)hKDM5A (JARID1A/RBP2)hKDM5B (JARID1B/PLU1)hKDM5C (JARID1C/SMCX)hKDM5D (JARID1D/SMCY)scKDM5 (Jhd2)spKDM5 (Jmj2)dKDM5 (Lid)

me

WSTF

K5

K119

ub

139

S

P

P

Y142

P

BRCT

ac

H2AXMst1ATMATR

PP4

EYA1/3

hKDM2A (JHDM1a/FBXL11)hKDM2B (JHDM1b/FBXL10)scKDM2 (Jhd1)hKDM4A (JMJD2A/JHDM3A)hKDM4B (JMJD2B)hKDM4C (JMJD2C/GASC1)scKDM4 (Rph1)dKDM2

NH2

O

C

N

N

NH2

O

OH

O

5caC

N

N

NH2

O

OH

5hmC

N

N

NH2

O

5mC

N

N

DNMT

TET

TET

TET

TDG,BER

AM-AR

AM-PD

TDG,BER

Rep

lica

tion Rep

lication

Replication

NH2

O

O

5fC

N

N

Epigenetic modificationsHistone modifications and DNA methylation are epigenetic marks that affect gene expression. This poster highlights the different types of epigenetic modifications and their writers, readers, and erasers.

Epigenetic modifications at AbcamTo help support your research, we have a range of resources, including sample preparation kits, detection assays for epigenetic modifications, recombinant active proteins, inhibitors of epigenetic pathways, and peptide array-tested monoclonal antibodies to histone modifications.

To find out more, check out our epigenetics content library where you can find more posters, guides, protocols, and webinars.

www.abcam.com/epigenetics

Histone modifications

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Acetylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Deimination

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

O-palmitoylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac Deacetylation P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Phosphorylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Ubiquitination

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Methylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Dephosphorylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

ADPRibosylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Demethylation

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Isomerization

Histone modifications binding proteins

Chromodomain proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

CHD1 H3K4me2/3

HP1/spSwi6 H3K9me2/3

spChp1 H3K9me2/3

CDY1 H3K9me2/3

PC1/PC2/PC/LHP1 H3K27me3, H3K9me3

MSL3 H3K36me3

hMRG15 H3K36me, H3K4me3

scEaf3 H3K36me, H3K4me3

CBX1,3,5 H3K9me3/2

MPP8 H3K9me3/2

Tip60 H3K9me3/2

CBX2,4,6,7,8 H3K27me3/2, H3K9me3/2

MBT proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac PHF20L1 H3K4me1, H4K20me1

SFMBT H3K9me1/2, H4K20me1/2

L3MBTL1 H4K20me1/2, H1bK26me1/2

L3MBTL1 H3K20me

L3MBTL1/2 H3K9me

MBTD1 H3K20me

PHD proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

scYng1 H3K4me2/3

ING1,2,3,4,5 H3K4me2/3

BPTF/dmNURF301 H3K4me2/3

scSpp1 H3K4me2/3

scSet3 H3K4me2/3

scJhd1 H3K4me3

RAG2 H3K4me3

TAF3 H3K4me3

ICBP90 (Np95) H3K9me2/3

JARID1C H3K9me3

JARID1A H3K4me2/3

KIAA1718 H3K4me2/3

Lid H3K4me2/3

MLL1 H3K4me2/3

PHF2,8 H3K4me2/3

PHO23 H3K4me2/3

Pygo H3K4me2/3

CHD4 H3K9me

ICBP90 H3K9me

SMCX H3K9me

DPF3 H3K14ac

14-3-3 proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

14-3-3 H3S10p, H3S28p

Tudor proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

JMJD2A H3K4me3, H4K20me3

53BP1 H4K20me1/2

spCrb2 H4K20me2

PHF20 H4K20me2

TDRD3 H3R17me2a, H4R3me2a

scSgf29 H3K4me2/3

PHF19 H3K36me3

PHF1 H3K36me3

Bromodomain proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Polybromo/BAF180 H3ac

scSnf2 H3ac, H4ac

Brd2,3,4,7 H3ac, H4ac

TAF1 H3ac, H4ac

P/CAF H3ac, H4ac, H4K16ac

CBP/p300 H3ac, H4ac

scBdf1 H4ac

hBRG1 H3K14ac

scRsc1,2,4 H3K14ac (Rsc4)

scGcn5 H4ac, H4K16ac

ATAD2 H3K14ac

BRDT H4K5ac, H4K8ac, H3K18ac

CBP/p300 H4K20ac, H3K36ac

GCN5 H4K16ac

PB-2 H3K14ac

TRIM24 H3K23ac

ZF-CW proteinsP

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

ZCWPW1 H3K4me1/2

ANKYRIN proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

G9a/GLP H3K9me2/1

PWWP proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

BRPF1 H3K36me3

DNMT3A H3K36me3

PDP1 H4K20me1

HDGF2 H3K36me3

BRCT proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

MDC1 H2AXS139p (γH2AX)

BIR proteins

P

P

14-3-3

ub

me

me

iso

cit

o-p

adp

PHD

bromo

MBT

tudor

chromo

ZF - CW

BIR

ANKYRIN

PWWP

BRCT

ac

ac

Survivin H3T3pCreated by Abcam in collaboration with Tony Kouzarides and Andy Bannister (Gurdon Institute, Cambridge).