fish heath management lab 5: histology (microanatomy) tarah johnson march 2 nd, 2009

Fish Heath Management Lab 5: Histology (microanatomy)

Tarah JohnsonMarch 2nd, 2009

Objectives

• Be able to microscopically identify cells and structures in the liver, kidney, and gills

• Have the opportunity to microscopically examine histological sections of other fish organs (eyes, head, muscle, etc.)

Liver

• Hepatocytes• Hepatic Sinusoid• Central Vein and or Hepatic Portal Vein• Bile Duct• Arteriole• Glycogen Vacuole

Central Vein and Hepatic Sinusoids

Erythrocytes visible within the central vein

Hepatic sinusoids

400x

Bile Duct Arteriole

A. Glycogen Vacuole

A

400x

Erythrocytes visible within arteriole

400x

Head Kidney

• Interrenal gland• Chromaffin cell• Postcardinal vein• Hematopoietic tissue

A

B

C

A. Interrenal Gland

B. Chromaffin Cell

C. Postcardinal vein

400x

A. Postcardinal vein

B. Hematopoietic tissue

40x

A B

Trunk Kidney

• Nephron (may not observe a complete nephron): Renal corpuscle, proximal tube, distal tubule, and collecting duct

• Renal Corpuscle:Glomerulus, Bowman’s capsule, basement membrane of glomerulus

• Melanocytes• Collecting duct and/or Mesonephric duct• Connective tissue

AB

C

A. Renal Corpuscle

B. Proximal Tubule

C. Distal Tubule

400x

400xA. Glomerulus

B. Basement membrane of glomerulus

C. Bowman’s capsule

A

C

B

A

B

A. Mesonephric Duct

B. Melanocytes

C. Connective tissue

400x

C

Gills

• Filament• Lamella• Goblet (or mucous) cell• Chloride cell• Pillar (or pilaster) cell• Gill filament cartilage

Gill filaments and lamellae

Lamella

A

A. Filament cartilage

B. Pillar cell

C. Chloride cell

B

40x

400x

C

Mucous or Goblet Cells

400x

Laboratory Steps

• Use proper microscope handling procedures (distributed in Lab 2)

• Examine histology slides of liver, kidney, and gills

• Examine additional histology slides of other organs

Be Sure to…

• Clean oil immersion lenses• Clean slides and return to proper case (the

same one you took it from)• Turn off microscopes and leave on low power

objective when done