giving a multidisciplinary video conference presentation

Giving a Multidisciplinary Video Conference

Presentation

Challenges

• Speaking to a camera

• Time delay when switching views

• Language accessibility

• Resolution of transmitted material– font size – complexity of material– slide format

Font Size:

14 pt is typical

28 pt minimumIf using 28 pt, Bold Helps

32-36 pt preferred

32-36 pt preferred

Use Plain Bold Fonts

Other: Probes to bacterial transcripts (28pt Arial Bold)

Other: Probes to bacterial transcripts (28pt Arial)

Other: Probes to bacterial transcripts (28 pt Times New Roman Bold)

Probe Type

Discovery: Genes of interest for particular species

Positive: Probes to bacterial transcripts that are spiked into total RNA

Negative: Probes to bacterial transcripts that are not spiked into the total RNA and are used for negative control threshold calculation

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Fiducials: Controls that the software package uses to grid the array.

Quality Flags

Good: No flags associated with this particular spot.

Empty: Spots near a background level where the mean background plus one standard deviation is greater than the signal mean.

MSR: Data points not provided (blank cell in excel worksheet) based upon quality control of manufacturing processes. Each array is scanned post-dispense and issues such as misfiring are labeled as MSR.

Poor: Spots identified based upon characteristics such as spot morphology or large interference in signal (such as a speck of dust).

Negative: Spots with an signal mean less than the background; intensity value is a negative number.

Typical presentation: 14 pt

Problems with Previous Slide

• Text too small

• Too much information

• Margins too small

• Language too complicated

Probe Type

Discovery: Genes of interest for particular species

Positive: Probes to bacterial transcripts that are spiked into total RNA

Negative: Probes to bacterial transcripts that are not spiked into the total RNA and are used for negative control threshold calculation

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Much easier to read: 28 ptBut still too complicated

Discovery: Genes of interest

Positive: Probes spiked into RNA

Negative: Probes not spiked into RNA

Less complicated, Larger marginsMakes point more effectively

Use Plain Contrasting Backgrounds

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Use Plain Contrasting Backgrounds

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Use Plain Contrasting Backgrounds

Black text on a plain white background has so much contrast it does not

project well.

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Use Plain Contrasting Backgrounds

A light coloured background helps but still has a high contrast.

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Use Plain Contrasting Backgrounds

Standard light yellow on dark blue works well

-As part of a pharmacogenetics project, we screened exons and flanking intronic regions for variation in a set of 24 membrane transporter genes (96 kb; 57% coding) in 247 DNA samples from ethnically diverse populations.

-We identified 680 Single Nucleotide Polymorphisms (SNPs), of which 175 were synonymous and 155 caused amino acid changes, and 29 small insertions and deletions.

-Amino acid diversity (NS) in transmembrane domains (TMDs) was significantly lower than in loop domains, suggesting that TMDs have special functional constraints.

-underlying assumption was that alleles that are functionally deleterious will be selected against and thus under represented at high frequencies and over represented at low frequencies.

-Green People had an excess of high frequency alleles in comparison to Blue People, consistent with a historic bottleneck. In addition, Blue People exhibited a much higher frequency of population specific medium-frequency alleles than did GreenPeople.

Another Bad Slide

Limit Page Content

Increase font size, Allow margins

Reduce content

Use no more that 6-8 lines per page

Allow no more that 4-5 major points per page

Remove jargon

- screened for inherited mutations in 24 genes from ethnically diverse samples

-680 mutations were identified

-155 of these caused amino acid changes

-assumption was that alleles that are functionally bad will be selected against

-Green people had more mutations than Blue

Simplifying Content can make it easier to present

Avoid Animations

Other: Probes to bacterial transcripts that are neither part of the negative control threshold calculation nor the positive controls

Some Problematic Slides:

Published Work this Summer

• In Vitro growth regulationand identification of potentialsignaling pathways in t(4;11)pediatric pre-B lymphoblastic leukemia (ALL)

• Abstract Submitted to the

American Society for Hematology

Annual Meeting 2004

35 KBJPEG

Background

Leads to > cell proliferation by activation of receptor

Results in: - < survival - > tumorigenicity - > invasiveness - > metastatic potential - altered drug sensitvity 31 KB

JPEG

Data / Analysis

Introduction

Background

Objectives

Methods

Sample Preparation

Data / Analysis

Conclusion

Acknowledgements

The analysis was based on paired samplesThe analysis was based on paired samples7 test does samples7 test does samples6 high does samples6 high does samples

To date, 35 paired patients have been run To date, 35 paired patients have been run 28 of which were analyzed this summer28 of which were analyzed this summer

Totaling 560 samplesTotaling 560 samples

The following is an example of a LC, The following is an example of a LC, ChromatographChromatograph

105KBPNGImage

Sensitivity of ALL to 17-AAG

0

50

100

0.01 0.1 1 1017-AAG Concentration (μM)

% S

urv

ival

as

com

par

ed t

o D

MS

O c

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Cell line 1Cell line 2Cell line 3Cell line 4

17-AAG17-AAG

Moving Towards a New EraMolecularly targeted therapies

• Studies have shown that behind every type of cancer cells there is a unique set of abnormal molecules that help with their survival and growth.

• We are also studying ways to specifically inactivate these molecules.

Binding of the drug to its target

DrugUnique cancer helping target protein

No treatment 1μM 17-AAG

3

4

1

2

• Again…– Simplify content– Use large plain fonts– Avoid complicated diagrams

No treatment 1μM 17-AAG

1

2

Moving Towards a New Era Molecularly targeted therapies

• Studies have shown that behind every type of cancer cells there is a unique set of abnormal molecules that help with their survival and growth.

Graphs

0

0 1 2 3 4 5 6

25

50

75

Time (min)

Thy

mid

ine

Upt

ake

(pm

ol/1

06 c

ells

)

Total

Equilibrative

Diffusion

Concentrative

+1mM TdR

+1mM Inosine

+1mM Uridine

0

0 1 2 3 4 5 6

25

50

75

Time (min)

Th

ymid

ine

Up

take

TotalEquilbrative

ConcentrativeDiffusion

Material that Projects Poorly

• gel • Flourescent image

If you must include complicated figures

• Give a copy of the powerpoint file to Cay or Kathryn the day before

• We will make and distribute copies

• Does not have to be the entire presentation

Summary-1

• Simplify content

• Avoid jargon

• Use 28 pt Arial bold as a minimum

• 6-8 lines of text per page

• 4-5 points per page

• Use a plain contrasting background

Summary-2

• Avoid animation• Avoid detailed or complicated figures

or pictures

• Do not use scanned material• Bring presentation on a CD that is

finalized (closed)• RELAX