hands-on approach to chromatography nataša gros 1 margareta vrtačnik 2, m. filomena camões 3 1...

HANDS-ON APPROACHHANDS-ON APPROACHTO CHROMATOGRAPHYTO CHROMATOGRAPHY

Nataša GrosNataša Gros11 Margareta Vrtačnik Margareta Vrtačnik22, M. Filomena , M. Filomena CamCamõõeses33

1 1 Faculty of Chemistry and Chemical Technology, Faculty of Chemistry and Chemical Technology, 22 Faculty of Natural Sciences and Engineering, Faculty of Natural Sciences and Engineering,

University of Ljubljana, SloveniaUniversity of Ljubljana, Slovenia3 3 University of Lisbon, PortugalUniversity of Lisbon, Portugal

Project No: SI/03/B/F/PP-176012© FKKT, Ljubljana, 2003-2005

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ExperimentExperiment

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LEGEND

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1. Introduction to chromatography

2. Gas chromatography

3. Liquid chromatography

4. Chromatography –fields of application

Examples of slides

A simplified gas chromatograph

Cylinder with carrier gas

Flow regulator

InjectorColumn with

stationaryphase

Detector

display

Record of

measurement values

-0.005

0

0.005

0.01

0.015

0.02

0.025

0.03

0.035

0 50 100 150 200 250 300 350 400 450 500

t /s

Odz

iv /

V

Chromatogram

Graphic presentation of measurement valuesvs time

Chromatographicpeak of the

component elutedfirst

Chromatographicpeak of the

component elutedsecond

RESPONSE

A simplified liquid chromatograph

Chromatographic system with vacuum water-jet pump

Containerwith

eluentPump

Sample injection

port

Columnwith

stationaryphase

Detector

display

Record of

measurement values

“Separating the dyes from mint aroma

Video: clickon the icon

“Separation of the dyes from mint aroma

Green component

blue component

AcknowledgementsThis Project has been carried out with the support of the

European Community. The content of this project does not necessarily reflect the

position of the European Community, or National Agency.

For this reason neither the National Agency nor the European Community take any responsibility regarding the content of the report.