have adipose derived stem cells potential to ...smartauci.com/pdf/praga.pdf · cells. 2. aim: the...
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1. INTRODUCTION: Construction of urinary bladder wall de novo for cancer patients with tissue engineering approach disqualify the use of autologous urothelial cells. 2. AIM: The goal of this study was to analyze if adipose derived stem cells (ADSCs) can transdifferentiate into urothelial- like cells (U-LCs) and serve potentially to construct urinary bladder wall in vitro.
The present work was supported by the National Center for Research and Development in Poland under Agreement no STRATEGMED1/235368/8/NCBR/2014 (Smart AUCI Project) within the Strategic Programme STRATEGMED “Prevention practices and treatment of civilization diseases”.
ADSCs+U-CM
NC+SM
NC+U-FM
PC+U-FM
Before the differen�a�on A�er 7 days A�er 14 days
Adipose derived stem cells (ADSCs)
+ a standard medium used for ADSCs culture (SM)
with the addi�on of urothelium derived condi�oned medium (U-CM)
= the differen�a�on (ADSCs + U-CM)
+ SM = the 1st nega�ve control (NC + SM)
+ SM with the addi�on of fresh medium used for UCs culture (U-FM)
= the 2nd NC (NC + U-FM)
HAVE ADIPOSE DERIVED STEM CELLS POTENTIAL TO TRANSDIFFERENTIATE INTO UROTHELIAL CELLS?
Buhl M, Rasmus M, Jundzill A, Balcerczyk D, Kloskowski T, Szeliski K, Dąbrowski P, Drewa T, Pokrywczynska M Department of Regenerative Medicine, Nicolaus Copernicus University in Torun, Ludwik Rydygier Medical College in Bydgoszcz, Poland
DIFFERENTIATION
7 OR 14 DAYS
ADSCs U-LCs?
3. MATERIALS AND METHODS:
DIFFERENTIATION: + a standard medium used for ADSCs culture (SM) with the addition of urothelium derived conditioned medium (U-CM) =ADSCs + U-CM
1st NEGATIVE CONTROL (NC): + SM =NC + SM
2nd NEGATIVE CONTROL (NC): SM with the addition of fresh medium used for urothelial cells (UCs) culture (U-FM) =NC + U-FM
ADSCs
UCs
4. RESULTS - CELL MORPHOLOGY:
0 7 14
AD
SCs
+ U
-CM
N
C +
SM
N
C +
U-F
M
PC
+ U
-FM
4. REAL-TIME PCR:
POSITIVE CONTROL (PC): + the standard, fresh medium used for UCs culture (U-FM) =PC + U-FM
ADSCs+
U-C
M
NC+S
M
NC+S
M
NC+U
-FM
PC+U
-FM
0
20
40
60
80
100
ADSCs+
U-C
M
NC+U
-FM
PC+U
-FM
14 days CK8
CK19
CK18
UP1b
CD44
CD105
Th
e r
ela
tive e
xp
ressio
n
0
20
40
60
80
100 7 days5. CONCLUSIONS: The study showed that ADSCs have no potential to transdifferentiate into urothelial- like cells and therefore there is a need to improve this method of transdifferentiation or search another type of stem cells to create urothelial cells for construction of urinary bladder wall de novo.
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