histocompatibility specialist curriculum 2018organizing committee chair jean heneghan, ms, chs...
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June 22-28, 2018Hyatt Regency Clearwater
Beach Resort & Spa CLEARWATER BEACH, FL
Registration will open in mid-JanuarySponsored by
American Foundation for Donation and Transplantation
American Foundation for Donation and TransplantationHistocompatibility Specialist Course Registration
Registration will open in mid-January.Please print or type.
Name: ___________________________________________________
Affiliation: ________________________________________________
Business Address: __________________________________________
_________________________________________________________
City, State, Zip: ____________________________________________
Phone: ___________________________________________________
Email: ___________________________________________________
Emergency Contact (Name & Phone): ________________________
_________________________________________________________
Check one: h Director h Laboratory Supervisorh New Laboratory Director h Technologisth Laboratory Manager h Other: _____________________
A check must accompany this form to ensure registration. Make checks payable to American Foundation for Donation and Transplantation. Credit cards are accepted.
Mail check & form to: AFDT, 8154 Forest Hill Avenue, Suite 3 Richmond, VA 23235-3255
Registration Fees (in US dollars)Before May 22 h $1,600.00After May 23 h $1,800.00Optional Review Session h $150.00(Mon., June 25, 12-2:30 pm)
Refunds will be made when requested in writing before April 30, 2018. A $100 fee will be charged on all refunds. There will be no refunds after May 15, 2018.
For information and registration, contact:Arlene M. Skinner, ManagerContinuing Education & Conference Planning8154 Forest Hill Avenue, Suite 3,Richmond, VA 23235-3255Tel: 804-323-9893; Fax: 804-323-1300; Email: skinner@amfdt.org
Note: Hotel and travel arrangements and their costs will be the responsibility of course participants. Registration will be Friday, June 22, 2018 and course will end on Thursday, June 28 at 12 noon. Breakfast, lunch and breaks will be provided each day except lunch on Monday, June 25, UNLESS you are participating in the optional review session. Box lunches will be provided for these participants. On Wednesday, June 27, dinner will be provided during a Farewell Gathering.
Reservations: Hyatt Regency Clearwater Beach Resort & Spa https://aws.passkey.com/go/HIST2018 Cutoff for group rate: June 1, 2018
Room rates: $199 single or double occupancy, plus 13% tax.
Highly recommended review:1. Reviews in Immunogenetics, Vol. 1 and Vol. 3, 1999, Munksgaard,
Copenhagen, Denmark.2. Handbook of Human Immunology. 1997 Leffell, Donnenberg and Rose,
CRC Press.3. Manual of Clinical Laboratory Immunology, Fifth Edition, 1997, eds:
Rose, deMacario, Fold, Lane and Nakamura, ASM Press, Section R: “Transplantation Immunology and Immunogenetics” and Zachary, AA and Steinberg, AG: Statistical Analysis and Applications of HLA Population Data, pp. 132-140.
4. The HLA Facts Book, 2000, Steven G.E. Marsh, Peter Parham and Linda D. Barber, Academic Press, San Diego, CA.
5. HIPAA (http://hhs.gov/news/press/2001/01privacy.html) pp. 1-50.
Recommended review:1. ASHI Laboratory Manual, 2001 4th Edition, Editors: Hahn, A., et al.,
(Pub. ASHI, 17000 Commerce Parkway, Suite C, Mount Laurel, NJ 08054).2. Flow Cytometry First Principles, 1992, Givan, Alice, (Pub. Wiley-Liss,
New York).3. Flow Cytometry Principles for Clinical Laboratory Practice: Quality
Assurance for Quantitative Immunophenotyping, 1995, Marilyn Owensand, Michael Loken (Pub.Wiley-Liss, New York).
4. “Flow Cytometric Crossmatching in Solid Organ Transplantation”, 1994, RA Bray, In Methods of Cell Biology: Flow Cytometry, Vol 41, Darzynkiewics, Z., Crissman, HZ, and Robinson, JP eds. Chapter 7, pp 103-119.
5. Bray R., Nickerson P., Kerman R., and Gebel H. Evolution of HLA Antibody Detection: Technology Emulating Biology. Immunologic Research 29:41-53, 2004.
6. Bray R.A. and Gebel H.M. Clinical Utility of Flow Cytometry in Allogeneic Transplantation. in; Flow Cytometry in Clinical Diagnosis, 4th Ed.; Carey J. McCoy J. and Keren D. eds. pgs. 275-294. ASCP Press, Chicago,IL. 2007
Reading for DNA & Stem Cell Lectures:1. Hurley, C. DNA methods for HLA typing. http://www.dodmarrow.org under
scientific services/educational material. 2008.2. Hurley, C. Acquisition and use of DNA-based HLA typing data in bone
marrow registries. Tissue Antigens 49:323, 1997.3. Holdsworth, R. et al. The HLA Dictionary 2008: a summary of HLA-A, -B,
-C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR and -DQ antigens. Tissue Antigens 73:95, 2009.
4. Marsh, S. et al. Nomenclature for factors of the HLA system, 2010. Tissue Antigens, in press.
5. Robinson, J. et al. IMGT/HLA and IMGT/MHC: sequence databases for the study of the major histocompatibility complex. Nucleic Acids Research 31:311, 2005.
6. ASHI Standards related to DNA-based HLA testing.7. Cutler, C. and J. Antin. An overview of hematopoietic stem cell transplanta-
tion. Clinics in Chest Medicine 26:517, 20058. Copelan, E. Hematopoietic stem-cell transplantation. New England Journal
Medicine 354:1813, 2006
Am
eric
an F
ound
atio
n fo
r D
onat
ion
and
Tra
nspl
anta
tion
8154
For
est
Hill
Ave
nue,
Sui
te 3
Ric
hmon
d, V
A 2
3235
-325
5
Pre
sort
edF
irst-
Cla
ss P
osta
ge
Pai
dR
ichm
ond,
VA
Per
mit
No.
681 Nineteenth
Histocompatibility Specialists Course
ABHI Approved
OR
GA
NIZ
ING
CO
MM
ITT
EE
Cha
irJe
an H
eneg
han,
MS,
CH
ST
elep
hone
: (50
4) 9
88-5
259
jhen
egh@
tula
ne.e
duT
ulan
e H
LA L
abor
ator
yN
ew O
rlea
ns, L
A
Inqu
irie
s ab
out
the
prog
ram
sho
uld
be a
ddre
ssed
to
Arl
ene
Skin
ner,
D
irec
tor
of O
pera
tion
s, 1
-800
-KID
NE
Y9,
ski
nner
@am
fdt.
org
PUR
POSE
: T
he c
urri
culu
m is
des
igne
d fo
r se
nior
tec
hnol
ogis
ts a
nd
new
labo
rato
ry d
irec
tors
invo
lved
in t
he d
ay t
o da
y m
anag
emen
t an
d de
velo
pmen
t of
the
his
toco
mpa
tibi
lity
labo
rato
ry. A
pplic
ants
sh
ould
hav
e a
back
grou
nd in
imm
unol
ogy,
imm
unog
enet
ics
and
hist
ocom
pati
bilit
y.
OB
JEC
TIV
E:
Pro
gram
obj
ecti
ve i
s to
est
abli
sh a
str
uct
ure
d
curr
icu
lum
lea
din
g to
:•
Rai
sin
g th
e st
anda
rds
of p
ract
ice
in c
lin
ical
h
isto
com
pati
bili
ty t
esti
ng.
• P
rovi
din
g a
fun
dam
enta
l ed
uca
tion
al p
rogr
am l
eadi
ng
to
AB
HI
His
toco
mpa
tibi
lity
Spe
cial
ist
Cer
tifi
cati
on (
CH
S).
• P
rom
otin
g ex
cell
ence
in
cli
nic
al l
abor
ator
y m
edic
ine.
SYL
LA
BU
S:
Eac
h s
tude
nt
wil
l re
ceiv
e a
com
preh
ensi
ve
cou
rse
syll
abu
s co
nta
inin
g:•
Lec
ture
su
mm
arie
s •
Ref
eren
ces
and
bibl
iogr
aph
y •
Pro
cedu
res
wit
h a
ppli
cati
ons
CU
RR
ICU
LU
M:
Cou
rse
pres
enta
tion
wil
l be
in
did
acti
c an
d
inte
ract
ive
disc
uss
ion
for
mat
. Th
e cu
rric
ulu
m f
ocu
s is
on
h
isto
com
pati
bili
ty t
esti
ng:
• T
heo
ry a
nd
prin
cipl
e •
App
lica
tion
•
Pro
cedu
re
• M
anag
emen
t A
PP
LIC
AN
T R
EQ
UIR
EM
EN
TS:
Tec
hn
olog
ist
in
His
toco
mpa
tibi
lity
Tes
tin
g, w
ith
a m
inim
um
of
3 ye
ars
wor
kin
g
expe
rien
ce a
nd/
or a
for
mal
cou
rse
in i
mm
un
olog
y or
gen
etic
s; o
r
a n
ew l
abor
ator
y di
rect
or; a
ctiv
e A
SHI
mem
bers
hip
(rec
omm
ende
d);
and
pre-
requ
isit
e re
adin
g co
mpl
eted
.
Thi
s co
urse
will
hav
e a
limit
ed n
umbe
r of
reg
istr
ants
; ear
ly r
egis
trat
ion
is r
ecom
men
ded.
HIST
OCOM
PATI
BILIT
Y SPE
CIAL
IST C
URRI
CULU
M 20
18
FRID
AY, JU
NE 22
Reg
istr
atio
n 5
:00
- 8:
00 p
m
SATU
RDAY
, JUNE
23B
asic
Con
cep
ts o
f
Tra
nsp
lan
tati
on I
mm
un
olog
y•
“Law
s” o
f T
ran
spla
nta
tion
• H
isto
ry &
Its
Im
pact
• A
dapt
ive
Imm
un
ity
& M
olec
ula
r B
asis
of
All
orec
ogn
itio
n•
Inn
ate
Imm
un
ity
Inn
ate
Imm
un
ity
• P
atte
rn R
ecog
nit
ion
Rec
epto
rs•
Infl
amm
atio
n•
Cyt
okin
es a
nd
Ch
emok
ines
• N
K C
ells
an
d N
K R
ecep
tors
Ad
apti
ve I
mm
un
ity
• Im
mu
nog
lobu
lin
& T
CR
Gen
e R
earr
ange
men
t•
TC
R, M
HC
& A
cces
sory
M
olec
ule
In
tera
ctio
ns
• T
Cel
l A
ctiv
atio
n &
Eff
ecto
r
T C
ells
• B
Cel
l A
ctiv
atio
n &
An
tibo
dy
Pro
duct
ion
Mol
ecu
lar
Bio
logy
Tec
hn
iqu
es –
H
LA
Typ
ing
• D
NA
Str
uct
ure
an
d P
repa
rati
on,
Den
atu
rati
on/A
nn
eali
ng,
• O
ligo
nu
cleo
tide
Pro
be
Hyb
ridi
zati
on•
Lab
elin
g/D
etec
tion
• D
NA
Syn
thes
is; P
CR
; DN
A
Sequ
enci
ng
DN
A-B
ased
HL
A T
ypin
g M
eth
ods
• SS
OP
H; S
SP; S
BT
• T
ech
niq
ues
to
Iden
tify
H
LA
All
eles
HL
A I
nfo
rmat
ics
• A
cces
sin
g th
e T
ools
on
th
e IM
GT
/HL
A a
nd
Oth
er W
ebsi
tes
• M
atch
ing
wit
hin
a F
amil
y•
Res
olu
tion
of
Typ
ing
for
Un
rela
ted
Sear
ch•
Res
ult
s of
Un
rela
ted
Don
or /
C
ord
Blo
od S
earc
h•
Mis
mat
ched
Don
or B
ased
on
D
P E
pito
pe M
atch
ing
• R
evie
w H
omew
ork
on W
eb
Too
ls
Qu
alit
y A
ssu
ran
ce a
nd
Qu
alit
y C
ontr
ol f
or D
NA
-Bas
ed T
esti
ng
• V
ario
us
Step
s in
th
e D
NA
T
ypin
g P
roce
ss•
Pro
blem
Sol
vin
g
Hem
atop
oiet
ic S
tem
Cel
l T
ran
spla
nta
tion
• F
atal
Blo
od D
isea
ses
Tre
ated
w
ith
Tra
nsp
lan
tati
on•
HSC
tra
nsp
lan
tati
on P
roce
dure
• So
urc
es o
f St
em C
ells
• D
onor
Sel
ecti
on C
rite
ria
• D
onor
Sea
rch
Pro
cess
• L
abor
ator
y T
ests
-Pat
ien
t
& D
onor
Typ
ing
Cas
e St
ud
ies
SUND
AY, JU
NE 24
Imm
un
oreg
ula
tion
& T
oler
ance
• T
hym
ic S
elec
tion
• A
ctiv
atio
n v
s. I
nh
ibit
ion
: Si
gnal
s &
Pat
hw
ays
• A
popt
osis
an
d A
ctiv
atio
n-
Indu
ced
Cel
l D
eath
• C
lon
al D
elet
ion
• R
egu
lato
ry C
ells
HL
A D
isea
se A
ssoc
iati
ons
• C
har
acte
rist
ics
of H
LA
A
ssoc
iate
d D
isea
ses
• M
olec
ula
r M
ech
anis
ms
• O
ther
su
scep
tibi
lity
gen
es –
cy
toki
ne,
KIR
• C
omm
only
ord
ered
tes
ts f
or
Dis
ease
Ass
ocia
tion
• H
LA
Ass
ocia
tion
wit
h D
rug
Hyp
erse
nsi
tivi
ty
Gen
etic
Con
cep
ts A
pp
lica
ble
to
Im
mu
nog
enet
ics
• G
ene
Reg
ula
tion
an
d E
xpre
ssio
n•
Gen
e T
ran
smis
sion
– M
ende
lian
G
enet
ics
• G
enet
ic C
han
ge•
Gen
omic
s an
d P
rote
omic
s
Pop
ula
tion
Gen
etic
s an
d S
tati
stic
s•
Cal
cula
tion
of
Gen
e (A
llel
e)
Fre
quen
cies
(G
F)
and
Ph
enot
ype
Fre
quen
cies
(P
F)
• H
ardy
-Wei
nbe
rg (
HW
) T
esti
ng
• H
aplo
type
Est
imat
ion
an
d L
inka
ge D
iseq
uil
ibri
um
• T
ests
of
Sele
ctio
n•
Gen
etic
Dis
tan
ce•
Con
tin
gen
cy t
able
tes
tin
g
and
rela
ted
stat
isti
cs•
Log
isti
c R
egre
ssio
n
• F
amil
y-ba
sed
asso
ciat
ion
tes
ts
MH
C G
enet
ics
• M
HC
Gen
e C
ompl
ex –
Loc
i, G
ene
Cla
sses
, Non
-MH
C G
enes
, C
lass
I a
nd
Cla
ss I
I G
enes
• O
rgan
izat
ion
, Pro
duct
ion
-St
ruct
ure
, Exp
ress
ion
, R
egu
lati
on•
HL
A P
olym
orph
ism
, Var
iabl
e Si
tes,
Gen
erat
ion
, Mai
nte
nan
ce,
Lin
kage
Dis
equ
ilib
riu
m,
Cyt
okin
e G
ene
Pol
ymor
phis
m
MOND
AY, JU
NE 25
Soli
d O
rgan
Tra
nsp
lan
t Im
mu
nol
ogy
• A
llog
raft
Rej
ecti
on: t
ypes
an
d di
agn
osis
• C
lin
ical
App
roac
hes
to
Tol
eran
ce I
ndu
ctio
n•
Imm
un
omod
ula
tion
• P
ath
olog
y•
All
ogra
ft p
ath
olog
y•
Met
hod
s of
ass
essi
ng
graf
t fu
nct
ion
Cli
nic
al I
nte
rven
tion
in
T
ran
spla
nta
tion
• C
ause
s of
ESR
D, i
ndi
cati
ons
and
con
trai
ndi
cati
ons
for
tran
spla
nta
tion
• Im
mu
nos
upp
ress
ion
Pro
toco
ls:
stan
dard
an
d n
ew d
rugs
, ste
roid
sp
arin
g•
Tar
gets
of
Imm
un
osu
ppre
ssio
n•
Rol
e of
th
e la
bora
tory
in
su
ppor
tin
g cl
inic
al p
ract
ice
KP
D P
rogr
ams
Fre
e A
fter
noo
n a
nd
Eve
nin
g
Op
tion
al T
uto
rial
for
D
iplo
mat
e E
xam
(12:
00 p
m –
2:3
0 pm
)
TUES
DAY,
JUNE
26N
ext
Gen
erat
ion
Seq
uen
cin
g
Bas
ic P
rin
cip
les
of F
low
C
ytom
etry
Bas
ic C
once
pts
of
the
Flo
w
Ad
van
ced
Flo
w C
ytom
etry
• A
ppli
cati
on o
f F
low
Cro
ssm
atch
P
re a
nd
Pos
t T
ran
spla
nt
• F
low
Spe
cifi
city
Bea
ds•
Tes
t In
terp
reta
tion
• Q
C i
n F
low
Cyt
omet
ry
Com
ple
men
t-D
epen
den
t A
ssay
s•
CD
C A
ssay
s fo
r C
ross
mat
ch•
Inte
rfer
ence
wit
h E
xtri
nsi
c F
acto
rs•
C’ Q
C
Soli
d P
has
e Im
mu
noa
ssay
s•
Inte
rpre
tati
on I
ssu
es•
Pro
blem
s an
d P
itfa
lls
• D
efin
ing
Un
acce
ptab
le A
nti
gen
s•
Vir
tual
Cro
ssm
atch
es
Pat
ien
t A
nti
bod
y Is
sues
• R
elev
ance
of
An
tibo
dies
an
d A
nti
body
Ch
arac
teri
stic
s•
Pu
blic
, CR
EG
, Epi
tope
s•
Au
to-A
nti
bodi
es•
Non
-HL
A A
bs•
Sign
ific
ance
of
C4d
dep
osit
ion
• C
4d a
nd
C1q
ass
ays
Ep
itop
e A
nal
ysis
An
tibo
dy
/ X
M C
ase
Stu
die
s
WED
NESD
AY, JU
NE 27
An
tibo
die
s: M
ech
anis
m o
f A
ctio
n
& T
ools
• A
nti
body
an
d C
ompl
emen
t•
C’ A
ctiv
atio
n a
nd
Acc
omm
odat
ion
• E
ndo
thel
ial
Cro
ssm
atch
• T
etra
mer
s fo
r Q
uan
tify
ing
B
Cel
ls
Tra
nsp
lan
tin
g th
e Se
nsi
tize
d
Pat
ien
t•
Des
ensi
tiza
tion
• P
osit
ive
Cro
ssm
atch
• P
lam
aph
eres
is, I
VIg
• A
BO
In
com
pati
ble
• R
isk
Fac
tors
for
An
tibo
dy
Med
iate
d R
ejec
tion
• D
onor
Kid
ney
Exc
han
ge
Ph
arm
acog
enet
ics
Ch
imer
ism
Tes
tin
g•
Met
hod
s u
sin
g ta
nde
m r
epea
ts
(ST
R/V
NT
R)
• D
eter
min
ing
chim
eris
m i
n c
ell
lin
eage
s•
Oth
er m
eth
ods
All
ocat
ion
of
Org
ans
from
D
ecea
sed
Don
ors
• N
ew /
Upd
ate
OP
TN
Pol
icie
s
for
HL
A T
ypin
g/K
PD
• R
ecen
t A
dvan
ces
in C
PR
A
and
HL
A T
ypin
g•
Impa
ct o
f N
ew K
idn
ey
All
ocat
ion
Sys
tem
Em
ergi
ng
Tec
hn
olog
ies
in t
he
HL
A L
abor
ator
y –
Spon
sor
Pre
sen
tati
ons
THUR
SDAY
, JUNE
28Im
ple
men
tin
g an
Eff
ecti
ve Q
A
Pro
gram
• D
esig
nin
g a
QA
Pro
gram
• Q
ual
ity
Indi
cato
rs a
nd
Mon
itor
ing
• R
oot
Cau
se A
nal
ysis
• Q
AP
I•
Com
pete
ncy
Ass
essm
ent
Lab
orat
ory
Acc
red
itat
ion
• In
spec
tion
Pro
cess
– A
SHI
an
d C
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ther
Reg
ula
tory
Age
nci
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CM
S, J
C, U
NO
S, N
MD
P,
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A, F
DA
Lab
orat
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Man
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Lab
orat
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Safe
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Ship
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Spec
imen
Han
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D.
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ecto
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Labo
rato
ry, I
nc.
Nas
hvill
e, T
N
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