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If you have questions check out our facebook group!!
http://www.facebook.com/groups/350201601657281/
Jacob Matsonmatsonj@uci.edu
Energetics• Enzymes stabilize transition state, lower activation energyGibbs free energy not at equilibrium:• ∆G = ∆G° + RTlnQ R=8.314 J/(K*mol) Q = [product]/[reactant]At equilibrium:∆G = ∆G° + RTlnKeq ∆G = 0, easy to solve for Keq
Energy Coupling• Link an unfavorable reaction (+ ∆G)with a favorable one (- ∆G) • Ex: many unfavorable reactions are coupled with ATP hydrolysis
∆H: Enthalpy: heat given off∆S: Entropy: how disordered something is
∆G = ∆H - ∆TS• A reaction has a ∆H = 0.5 kcal/mol, ∆S of 0.001 kcal/mol at 27 C• What will ∆G be? Above what temperature will the reaction be
favorable?∆G = 0.5-300*.001=.20 = .5-T*.001 T=500K
Pyruvate dehydrogenasename product
E1 pyruvate dehydrogenase CO2
E2 dihydrolipoamide acetyltransferaseAcetyl CoAE3 dihydrolipoamide dehydrogenaseNADH
Prosthetic groups E1 TPP – thiamine pyrophosphate
E2 lipoic acid
E3 FAD
Note: overall reaction is Oxidative decarboxylation
Where is each vitamin found?
B1: thiamine -> TPP
B2: riboflavin -> FAD
B3: niacin -> NAD
B5: pantothenic acid -> coenzyme A
Which of these does not inhibit deactivation of pyruvate dehydrogenase (E1)?
A. Ca2+ calcuim activates the phosphatase, releasing inactivation
B. NADC. CoAD. ADPE. Pyruvate
What are the products of the TCA cycle (1 cycle)?
2CO2, 1 ATP or GTP 1QH2, 3NADH
How many ATP are made per NADH /QH2?
2.5 per NADH1.5 per QH2
How many ATP are made by oxidation of 1 glyceraldehyde-3-phosphate?
Glycolysis= 2 atp + 2.5 from nadh+2.5 from nadh pyruvate carboxylase+ 10 from TCA=17 ATP
What enzyme catalyzes formation of the following molecule?
Succinyl-coa synthetase
Succinyl-coA to succinate
Makes one GTP/ATP
Draw the product of the TCA enzyme linked to the electron transport chain.
Succinate to fumarateBySuccinate dehydrogenase
AkaComplex IIIn
Which enzymes (TCA Cycle) produce NADH + CO2 through oxidative
decarboxylation?
Isocitrate dehydrogenase(isocitrate to alphaketoglutarate)
Alphaketoglutarate dehydrogenase(alphaketoglutarate to succinyl coa)
Draw the product of the final oxidative step of the TCA cycle.
Malate dehydrogenaseMalate to oxaloacetateMakes nadh
Which of these does not inhibit citrate synthase?
A. ATPB. Succinyl-CoAC. NADHD. IsocitrateE. They all inhibit it
You discover a new species with 5 electron transport chain proteins. In what order will
electrons flow through these proteins? E0’
A 0 VB 1 VC -1 VD 3 VE -2 V
Start: E C A B D end
How many protons are pumped across each complex of the ETC?
I: 4 NADHII: 0 succinateIII: 4 IV: 2Where do NADH / Succinate drop off electrons?
How many hydrogens need to be transported across the inner membrane to make 1 ATP?
4
3 go through atp synthase1 needed for symporter of H and Pi
How many hydrogens are pumped by the ETC per oxidized glucose molecule?
10 hydrogens per nadh6 per qH2Glycolysis: 2nadhPyruvate dehydrogenase: 2 nadhTca: 6 nadh 2 QH2Total 10 nadh 2 qh2 100 + 12 = 112 hydrogens
FILL OUT YOUR EVALS PLEASE
GOOD LUCK WITH THE FINAL!!!!!!
Tired of studying? Watch these videos!!
Glycolysis regulationhttp://www.youtube.com/watch?v=LHMLHCx0ASM&t=2m20sHey There Pyruvatehttp://www.youtube.com/watch?v=6TOdcaNw3IAMaking ATPhttp://www.youtube.com/watch?v=YyN0wx2AHfE&feature=relatedOxidate it or Love ithttp://www.youtube.com/watch?v=VCpNk92uswY
If you want them, my answers will also be posted online at the link below within a day after this review. I recommend you try each problem first before seeing the answer to test how much you know.
https://webfiles.uci.edu/matsonj/Winter2012/98reviewanswers.ppt
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